CN105830884A - Ecological-simulated water-surface cultivation method for Anoectochilus roxburghii - Google Patents
Ecological-simulated water-surface cultivation method for Anoectochilus roxburghii Download PDFInfo
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- CN105830884A CN105830884A CN201610186069.7A CN201610186069A CN105830884A CN 105830884 A CN105830884 A CN 105830884A CN 201610186069 A CN201610186069 A CN 201610186069A CN 105830884 A CN105830884 A CN 105830884A
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- herba anoectochili
- anoectochili roxburghii
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- 241000934230 Anoectochilus roxburghii Species 0.000 title abstract description 12
- 238000012364 cultivation method Methods 0.000 title abstract 3
- 239000003337 fertilizer Substances 0.000 claims abstract description 33
- 238000000034 method Methods 0.000 claims abstract description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 69
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 12
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 10
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 10
- 150000002333 glycines Chemical class 0.000 claims description 10
- 210000000582 semen Anatomy 0.000 claims description 10
- 230000004720 fertilization Effects 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 claims description 6
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims description 6
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 6
- 239000004327 boric acid Substances 0.000 claims description 6
- 229910000365 copper sulfate Inorganic materials 0.000 claims description 6
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 6
- 238000003306 harvesting Methods 0.000 claims description 6
- 229940099596 manganese sulfate Drugs 0.000 claims description 6
- 239000011702 manganese sulphate Substances 0.000 claims description 6
- 235000007079 manganese sulphate Nutrition 0.000 claims description 6
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 6
- 239000004323 potassium nitrate Substances 0.000 claims description 6
- 235000010333 potassium nitrate Nutrition 0.000 claims description 6
- 239000011684 sodium molybdate Substances 0.000 claims description 6
- 235000015393 sodium molybdate Nutrition 0.000 claims description 6
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims description 6
- 239000000243 solution Substances 0.000 claims description 6
- 239000004471 Glycine Substances 0.000 claims description 5
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 claims description 5
- 238000004090 dissolution Methods 0.000 claims description 5
- 239000000706 filtrate Substances 0.000 claims description 5
- 229960000367 inositol Drugs 0.000 claims description 5
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 claims description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 5
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 5
- 235000011837 pasties Nutrition 0.000 claims description 5
- ZUFQODAHGAHPFQ-UHFFFAOYSA-N pyridoxine hydrochloride Chemical compound Cl.CC1=NC=C(CO)C(CO)=C1O ZUFQODAHGAHPFQ-UHFFFAOYSA-N 0.000 claims description 5
- 229960004172 pyridoxine hydrochloride Drugs 0.000 claims description 5
- 235000019171 pyridoxine hydrochloride Nutrition 0.000 claims description 5
- 239000011764 pyridoxine hydrochloride Substances 0.000 claims description 5
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 5
- 230000008859 change Effects 0.000 claims description 4
- 239000011790 ferrous sulphate Substances 0.000 claims description 4
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 4
- 238000005286 illumination Methods 0.000 claims description 4
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 4
- 229960000344 thiamine hydrochloride Drugs 0.000 claims description 4
- 235000019190 thiamine hydrochloride Nutrition 0.000 claims description 4
- 239000011747 thiamine hydrochloride Substances 0.000 claims description 4
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 4
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 4
- 229960001763 zinc sulfate Drugs 0.000 claims description 4
- 238000011144 upstream manufacturing Methods 0.000 claims description 3
- 229940079232 zinc sulfate 15 mg Drugs 0.000 claims description 2
- 239000007921 spray Substances 0.000 claims 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims 2
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 claims 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 claims 1
- 238000010790 dilution Methods 0.000 claims 1
- 239000012895 dilution Substances 0.000 claims 1
- 229940124274 edetate disodium Drugs 0.000 claims 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims 1
- 239000001117 sulphuric acid Substances 0.000 claims 1
- 235000011149 sulphuric acid Nutrition 0.000 claims 1
- 229960003495 thiamine Drugs 0.000 claims 1
- 235000019157 thiamine Nutrition 0.000 claims 1
- 239000011721 thiamine Substances 0.000 claims 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 abstract description 5
- 230000012010 growth Effects 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 230000004083 survival effect Effects 0.000 description 12
- 238000002054 transplantation Methods 0.000 description 6
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 description 4
- 241000592344 Spermatophyta Species 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 241000719837 Anoectochilus Species 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 206010016807 Fluid retention Diseases 0.000 description 2
- 230000003698 anagen phase Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 206010012601 diabetes mellitus Diseases 0.000 description 2
- 239000006260 foam Substances 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 150000002505 iron Chemical class 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000012266 salt solution Substances 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 206010008909 Chronic Hepatitis Diseases 0.000 description 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical group [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000009305 arable farming Methods 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Inorganic materials [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 description 1
- 229940097267 cobaltous chloride Drugs 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000008175 fetal development Effects 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000003914 insulin secretion Effects 0.000 description 1
- 210000004153 islets of langerhan Anatomy 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 1
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 201000008383 nephritis Diseases 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000009923 sugaring Methods 0.000 description 1
- 231100000033 toxigenic Toxicity 0.000 description 1
- 230000001551 toxigenic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Fertilizers (AREA)
Abstract
The invention relates to an ecological-simulated water-surface cultivation method for Anoectochilus roxburghii. The method comprises the steps of selecting an ecological-simulated environment, constructing ecological-simulated facilities, preparing a foliar fertilizer for the Anoectochilus roxburghii and carrying out a fertilizing method. According to the method, the aim of ecological-simulated Anoectochilus roxburghii cultivation is achieved through selecting the ecological environment suitable for the growth of the Anoectochilus roxburghii and adding some simple cultivation facilities; and the special foliar fertilizer for the Anoectochilus roxburghii is prepared, and the fertilizing method is explored, so that the cost is reduced, and the maximization of benefit is achieved. According to the ecological-simulated water-surface cultivation method for the Anoectochilus roxburghii, disclosed by the invention, the cultivation cost of the Anoectochilus roxburghii is lower, nursery-grown plants are more robust, and the quality is higher. The weight of plants and planted pots thereof is undertaken by floating bodies, and other ancillary facilities are not required, so that the cost is low, the mounting is convenient, and the management is facilitated. A very critical practical technique is provided for the production of the Anoectochilus roxburghii.
Description
Technical field
The present invention relates to a kind of arable farming method, a kind of method being specifically related to Ecology water-surface cultivation Herba Anoectochili roxburghii, belong to technical field of crop cultivation.
Background technology
Herba Anoectochili roxburghii Anoectochilus roxburghii has another name called Anoectochilus nefiliforme (Nakai) hara, Herba Melicae Scabrosae, for the perennial rare Chinese medicine of the orchid family Anoectochilus Blume, main product in Fujian, Zhejiang, TaiWan, China, Jiangxi, the ground such as Guizhou, there is the effect such as clearing away heat and cooling blood, removing dampness and detoxicating, for treating diabetes, nephritis, acute, chronic hepatitis etc., in the laudatory title enjoying " king of medicine " among the people.Modern study shows in Herba Anoectochili roxburghii containing anoectochilus roxburghii glycosides isoreactivity composition, there is reparation damaged islet cells, recover the pharmacologically active of normal insulin secretion, the Chinese patent medicine with Herba Anoectochili roxburghii as primary raw material, such as compound Jin Anoectochilus roxburghii capsule, have been used for the treatment of diabetes clinically.Owing to Herba Anoectochili roxburghii seed is small, fetal development is incomplete, and under natural conditions, breeding potential is extremely low, develops through for many years, and current wild resource has been on the verge of exhaustion.Protection and the effective way of its wild resource of rational exploitation and utilization is just become with tissue-culturing rapid propagation and transplantation technique large scale cultivating Herba Anoectochili roxburghii.
The quick breeding technology of artificial organ cultivation at present is gradually improved, substantial amounts of Herba Anoectochili roxburghii test tube Seedling can have been cultivated under laboratory artificial condition, therefore on the premise of solving source of seedling, practice in transplantation of seedlings technology just becomes a period of time from now on the emphasis studied, and large-scale production at present there is also the problems such as test tube transplantation of seedlings survival rate is the highest, upgrowth situation is undesirable, high expensive.
Summary of the invention
A kind of method that it is an object of the invention to provide Ecology water-surface cultivation Herba Anoectochili roxburghii.
It is an object of the invention to be realized by the following method.
The method of a kind of Ecology water-surface cultivation Herba Anoectochili roxburghii of the present invention, it is characterised in that step is as follows:
1. select Ecology environment: select height above sea level to be not less than 400 meters, January temperature on average >=10 DEG C, July temperature on average≤27 DEG C, air humidity >=70%, water surface planting under the small stream limit tree shade that illumination straight fluidic time is short, or the vacant lot utilizing the drop of physical features to introduce the water into periphery carries out facility cultivation；
2. Ecology facility cultivation:
Selecting the water surface under the tree shade of small stream limit is Ecology environment, carries out facility cultivation；Or on the vacant lot on small stream limit, put up hot house, big ceiling adds shading screen；Building pond in canopy, the stream of upstream enters pond water-retention from water inlet, and unnecessary water flows into the stream in downstream from outlet；Utilize the artificial Ecology environment arranged, carry out facility cultivation；Described facility cultivation: connecting into furrow with buoyancy aid by rope on the water surface, the two ends of furrow are articulated on fixing mark post, and buoyancy aid fluctuates along with the change of water level；Having multiple plantation aperture on buoyancy aid, hole diameter is 1cm；During plantation Herba Anoectochili roxburghii, including in aperture after being encased with pasture and water by the root of its bottle of Seedling, planting density is 4cm × 4cm；
3. Herba Anoectochili roxburghii foliage fertilizer preparation:
A, after claiming the Semen Glycines of 100-110g to be soaked in water overnight, takes out the water that Semen Glycines adds 2-3ml, broken into pasty state with pulverizer, load container and add water to 900ml, sealing, standing no less than 1 month, then by two layers of filtered through gauze, taking filtrate water, to be settled to 1000ml standby；
B, claims potassium nitrate 440-460mg, ammonium nitrate 340-360mg, potassium dihydrogen phosphate 49-51mg, magnesium sulfate 79-81mg, boric acid 14-16mg, manganese sulfate 28-32mg, zinc sulfate 14-16mg, sodium molybdate 2.3-2.6mg, copper sulfate 0.23-0.26mg, disodiumedetate 37.2-37.4mg ferrous sulfate 27.6-27.9mg inositol 210-230mg, glycine 2-2.2mg, thiamine hydrochloride 0.1-0.12mg, mix after pyridoxine hydrochloride 0.4-0.6mg water dissolution and be settled to 10L；
C. before using, A, B solution are mixed by weight the ratio for 1:10, be Herba Anoectochili roxburghii foliage fertilizer；
4. fertilizing method:
Herba Anoectochili roxburghii bottle seed starts fertilization of leaves after planting 15 days, is sprayed on blade face, sprayed once every 10-15 days again after being diluted with the water of 4 times of weight by Herba Anoectochili roxburghii foliage fertilizer；
After Herba Anoectochili roxburghii bottle seed plants 45 days, it is sprayed on blade face after Herba Anoectochili roxburghii foliage fertilizer is diluted with the water of 2 times of weight, afterwards, sprayed once every 10-15 days, until harvest.
The application effect of the present invention:
1. the different cultivation condition impacts on Herba Anoectochili roxburghii bottle transplantation of seedlings survival rate
Herba Anoectochili roxburghii is middle heliophobous plant, likes the low light level, high humidity, nice and cool transplanting environment.The growth preference temperature of Herba Anoectochili roxburghii is at 20～24 DEG C, and high temperature is easily caused death.Utilizing air-flow method to measure, Herba Anoectochili roxburghii light saturation point is 5000Lx, and light compensation point is 400Lx, and maximum light requirement is less than 6000Lx.According to these conditions, selecting in Fuzhou City, Fujian Province Min Hou Xian Qishan tourist district, height above sea level 400 meters, forest carries out lid rate height, and summer temperature is felt nice and cool, and there is wild Herba Anoectochili roxburghii locality, and ecological environment is well suited for the growth of Herba Anoectochili roxburghii.Water surface planting under the small stream limit tree shade that illumination straight fluidic time is short.
Being buoyancy aid with foam plastics, connect into furrow with rope between buoyancy aid, an iron ring is installed at the two ends of furrow, is enclosed within fixing mark post, and buoyancy aid can change along with the change of water level.Foam plastics is provided with multiple implantation hole, and aperture is 1cm.The root of Herba Anoectochili roxburghii bottle Seedling is included in aperture after encasing with pasture and water.Planting density is 4cm × 4cm.
In order to relatively different cultivation conditions are on Herba Anoectochili roxburghii bottle transplantation of seedlings survival rate and the impact of increment.We devise three kinds of different cultivation conditions, and comparative result is as shown in table 1.Wherein, the substrate of substrate culture is peat soil: husky 1:1 (weight ratio)；Water circulating cultivation is to utilize water pump to carry out water circulation in booth.Survival rate and yield is added up after planting 6 months.
Result of the test shows, the Ecology water-surface cultivation of the present invention and the survival rate of water circulating cultivation are substantially high than substrate culture, so yield is the highest much.
The different cultivation condition impact on Herba Anoectochili roxburghii bottle transplantation of seedlings survival rate of table 1
The variable concentrations of table 2. foliage fertilizer and the impact on Herba Anoectochili roxburghii different growth phases of the Different Fertilization mode
2. the variable concentrations of foliage fertilizer and the impact on Herba Anoectochili roxburghii different growth phases of the Different Fertilization mode.
In order to find suitable foliage fertilizer concentration, fertilizing method and interval time, do three factors, the orthogonal test of three levels by L9 (3) 4 orthogonal table.Each process combines kind of 200 strains, adds up gross weight after 6 months.Result of the test is as shown in table 2, and the concentration of foliage fertilizer is on survival rate impact maximum, and concentration more high-survival rate is the lowest.Due to nursery stock death the most in early days, so, the foliage fertilizer using low concentration in early days is preferable.In terms of the interval time of fertilising, preferably 10 days and 15 days, survival rate is all more than 94%.From the point of view of ultimate output, preferably the concentration of 1/2, average product is at 260.7 grams.Fertilising interval time be also preferably 10 days and 15 days. consider each factor, bottle seed plant 15 days after with 1/4 foliage fertilizer, every 15 days sprinkling once, continuous 2 times.Then with 1/2 nutritional solution, sprayed once every 15 days, until harvest.1/2,1/4 concentration described in table 2, refers to dilute foliage fertilizer with the water of 2 times, 4 times weight.
3. use different foliage fertilizer to Herba Anoectochili roxburghii survival rate and the impact of yield
Formula and the classical improvement Huo Gelande formula of selecting MS culture medium do individual comparison with inventive formulation, after bottle seed plants 15 days, every fertilising in 15 days once, and continuous 10 times.Result of the test is as shown in table 3, and concentration and survival rate have obvious negative correlation.From the point of view of foliage fertilizer kind, or it is best with inventive formulation, either survival rate, or yield is all apparently higher than other two kinds of formula.
The different foliage fertilizer of table 3 is on Herba Anoectochili roxburghii survival rate and the impact of yield
* MS is 1962, MS culture medium disclosed in Murashige and Skoog, not sugaring and agar powder.
* improves Huo Gelan formula: four water-calcium nitrate 945mg/L, potassium nitrate 506mg/L, ammonium nitrate 80mg/L, potassium dihydrogen phosphate 136mg/L, Magnesium sulfate heptahydrate 493mg/L, iron salt solutions 2.5ml, liquid microelement 5ml, pH=6.0.
Iron salt solutions: ferrous sulfate heptahydrate 2.78g, disodiumedetate 3.73g, distilled water 500ml, pH=5.5.
Liquid microelement: potassium iodide 0.83mg/l, boric acid 6.2mg/L, manganese sulfate 22.3mg/L, zinc sulfate 8.6mg/L, sodium molybdate 0.25mg/L, copper sulfate 0.025mg/L, cobaltous chloride 0.025mg/L.
There is advantages that
1. the method using a kind of Ecology water-surface cultivation Herba Anoectochili roxburghii of the present invention, can be with simplified cultivation link, compared with water planting, saves water pump and the power consumption of water circulation, thus reduces cultivation cost.
2. the present invention mainly utilizes Herba Anoectochili roxburghii Root Absorption to utilize nitrogen, P elements to carry out metabolic activity, can remove Water, phosphorus nutrition material, reach the effect of purifying water body.
3. the present invention is simple to operate, practical, small investment, is suitable for there is being the area popularization of wild Herba Anoectochili roxburghii.
The present invention, by selecting to be suitable for the ecological environment of Herba Anoectochili roxburghii growth, adds some simple cultivating facilities, reaches the purpose of Emulational culture Herba Anoectochili roxburghii, by preparing Herba Anoectochili roxburghii special-purpose foliage fertilizer and exploring fertilizing method, to reduce cost, reaches the maximization of benefit.The method of the Ecology water-surface cultivation Herba Anoectochili roxburghii of the present invention, the toxigenic capacity making Herba Anoectochili roxburghii is lower, and nursery stock is more strong, and quality is more preferable.Due to plant and plant plant basin body weight all undertaken by buoyancy aid self, without other affiliated facility, its low cost, easy for installation and be easy to management.Produce for Herba Anoectochili roxburghii and provide the most crucial practical technique.
Detailed description of the invention
In order to be further elucidated with the present invention rather than limit the present invention, it is illustrated below in conjunction with example.
Embodiment one: a kind of method of Ecology water-surface cultivation Herba Anoectochili roxburghii, comprises the following steps:
1, Ecology environment is selected:
Select height above sea level be not less than 400 meters, January temperature on average >=10 DEG C, July temperature on average≤27 DEG C, air humidity >=70%, the water surface planting under the small stream limit tree shade that illumination straight fluidic time is short；
2. Ecology facility cultivation:
Selecting the water surface under the tree shade of small stream limit is Ecology environment, carries out facility cultivation；
3. Herba Anoectochili roxburghii foliage fertilizer preparation:
A, after claiming the Semen Glycines of 100g to be soaked in water overnight, taking-up 2g Semen Glycines adds the water pulverizer of 2g and is broken into pasty state, load container and add water to 900ml, sealing, stand no less than 1 month, then by two layers of filtered through gauze, taking filtrate, to be settled to 1000ml with tap water standby；
B, claims potassium nitrate 450mg, ammonium nitrate 350mg, potassium dihydrogen phosphate 50mg, magnesium sulfate 80mg, boric acid 15mg, manganese sulfate 30mg, zinc sulfate 15mg, sodium molybdate 2.5mg, copper sulfate 0.25mg, disodiumedetate 37.3mg ferrous sulfate 27.8mg inositol 200mg, glycine 2mg, thiamine hydrochloride 0.1mg, mix after pyridoxine hydrochloride 0.5mg water dissolution and be settled to 10L；
C. before using, A, B solution are mixed by weight the ratio for 1:10, be Herba Anoectochili roxburghii foliage fertilizer；
4. fertilizing method:
Herba Anoectochili roxburghii bottle seed starts fertilization of leaves after planting 15 days, is sprayed on blade face, sprayed once every 10 days again after being diluted with the water of 4 times of weight by Herba Anoectochili roxburghii foliage fertilizer；
After planting 45 days, it is sprayed on blade face after Herba Anoectochili roxburghii foliage fertilizer is diluted with the water of 2 times of weight, afterwards, sprayed once every 10 days, until harvest.
By the method for the present embodiment, transplant Herba Anoectochili roxburghii bottle Seedling 1000 strain, after 6 months, results Herba Anoectochili roxburghii fresh weight 1.428 kilograms altogether.
Embodiment two: a kind of method of Ecology water-surface cultivation Herba Anoectochili roxburghii, comprises the following steps:
1, select Ecology environment: select height above sea level be not less than 400 meters, January temperature on average >=10 DEG C, July temperature on average≤27 DEG C, air humidity >=70%, the vacant lot utilizing the drop of physical features to introduce the water into periphery carries out facility cultivation；
2. Ecology facility cultivation:
Select on the vacant lot on small stream limit, put up hot house, big ceiling adds shading screen；Building pond in canopy, the stream of upstream enters pond water-retention from water inlet, and unnecessary water flows into the stream in downstream from outlet；Utilize the artificial Ecology environment arranged, carry out facility cultivation；
3. Herba Anoectochili roxburghii foliage fertilizer preparation:
A, after claiming the Semen Glycines of 110g to be soaked in water overnight, taking-up 2g Semen Glycines adds the water pulverizer of 2g and is broken into pasty state, load container and add water to 900ml, sealing, stand no less than 1 month, then by two layers of filtered through gauze, taking filtrate, to be settled to 1000ml with tap water standby；
B, claims potassium nitrate 460mg, ammonium nitrate 360mg, potassium dihydrogen phosphate 51mg, magnesium sulfate 81mg, boric acid 16mg, manganese sulfate 31mg, zinc sulfate 16mg, sodium molybdate 2.6mg, copper sulfate 0.26mg, disodiumedetate 37.4mg ferrous sulfate 27.9mg inositol 230mg, glycine 2.1mg, thiamine hydrochloride 0.12mg, mix after pyridoxine hydrochloride 0.6mg water dissolution and be settled to 10L；
C. before using, A, B solution are mixed by weight the ratio for 1:10, be Herba Anoectochili roxburghii foliage fertilizer；
4. fertilizing method:
Herba Anoectochili roxburghii bottle seed starts fertilization of leaves after planting 15 days, is sprayed on blade face, sprayed once every 15 days again after being diluted with the water of 4 times of weight by Herba Anoectochili roxburghii foliage fertilizer；
After planting 45 days, it is sprayed on blade face after Herba Anoectochili roxburghii foliage fertilizer is diluted with the water of 2 times of weight, afterwards, sprayed once every 15 days, until harvest.
By the method for the present embodiment, transplant Herba Anoectochili roxburghii bottle Seedling 1000 strain, after 6 months, results fresh weight 1.643 kilograms altogether.
Claims (3)
1. the method for an Ecology water-surface cultivation Herba Anoectochili roxburghii, it is characterised in that step is as follows:
(1) selecting Ecology environment: select height above sea level to be not less than 400 meters, January, temperature on average >=10 DEG C, put down July
All temperature≤27 DEG C, air humidity >=70%, water surface plantings under the small stream limit tree shade that illumination straight fluidic time is short, or utilize physical features
Drop introduce the water into the vacant lot of periphery and carry out facility cultivation；
(2) Ecology facility cultivation: selecting the water surface under the tree shade of small stream limit is Ecology environment, carries out facility cultivation；Or at small stream
Put up hot house on the vacant lot on limit, big ceiling adds shading screen；Building pond in canopy, the stream of upstream enters pond from water inlet and stores
Water, unnecessary water flows into the stream in downstream from outlet；Utilize the artificial Ecology environment arranged, carry out facility cultivation；Institute
Stating facility cultivation: connect into furrow with buoyancy aid by rope on the water surface, the two ends of furrow are articulated on fixing mark post, buoyancy aid along with
The change of water level and fluctuate；Having multiple plantation aperture on buoyancy aid, hole diameter is 1cm；During plantation Herba Anoectochili roxburghii, by it
The root of bottle Seedling is included in aperture after encasing with pasture and water, and planting density is 4cm × 4cm；
(3) Herba Anoectochili roxburghii foliage fertilizer preparation:
A, after claiming the Semen Glycines of 100-110g to be soaked in water overnight, takes out the water that Semen Glycines adds 2-3ml, is broken into pulverizer
Pasty state, loads container and adds water to 900ml, and sealing stands no less than 1 month, then by two layers of filtered through gauze, takes filtrate
It is settled to 1000ml standby with water；
B, claims potassium nitrate 440-460mg, ammonium nitrate 340-360mg, potassium dihydrogen phosphate 49-51mg, magnesium sulfate 79-81mg,
Boric acid 14-16mg, manganese sulfate 28-32mg, zinc sulfate 14-16mg, sodium molybdate 2.3-2.6mg, copper sulfate 0.23-0.26mg, second
Edetate disodium 37.2-37.4mg ferrous sulfate 27.6-27.9mg inositol 210-230mg, glycine 2-2.2mg, hydrochloric acid
Thiamine 0.1-0.12mg, mixes after pyridoxine hydrochloride 0.4-0.6mg water dissolution and is settled to 10L；
C. before using, A, B solution are mixed by weight the ratio for 1:10, be Herba Anoectochili roxburghii foliage fertilizer；
(4) fertilizing method:
Herba Anoectochili roxburghii bottle seed starts fertilization of leaves after planting 15 days, is sprayed at leaf after being diluted with the water of 4 times of weight by Herba Anoectochili roxburghii foliage fertilizer
On face, sprayed again once every 10-15 days；
After planting 45 days, it is sprayed on blade face, afterwards, every 10-15 after Herba Anoectochili roxburghii foliage fertilizer is diluted with the water of 2 times of weight
It sprays once, until harvest.
The method of a kind of Ecology water-surface cultivation Herba Anoectochili roxburghii the most according to claim 1, it is characterised in that described Herba Anoectochili roxburghii
Foliage fertilizer is prepared:
A, after claiming the Semen Glycines of 100g to be soaked in water overnight, taking-up Semen Glycines adds 2ml water pulverizer and is broken into pasty state, dress
Enter container and add water to 900ml, sealing, stand no less than 1 month, then by two layers of filtered through gauze, take filtrate water constant volume
Standby to 1000ml；
B, claims potassium nitrate 450mg, ammonium nitrate 350mg, potassium dihydrogen phosphate 50mg, magnesium sulfate 80mg, boric acid 15mg,
Manganese sulfate 30mg, zinc sulfate 15mg, sodium molybdate 2.5mg, copper sulfate 0.25mg, disodiumedetate 37.3mg sulphuric acid
Ferrous 27.8mg inositol 200mg, glycine 2mg, thiamine hydrochloride 0.1mg, after pyridoxine hydrochloride 0.5mg water dissolution
Mix and be settled to 10L；
C. before using, A, B solution are mixed by weight the ratio for 1:10, be Herba Anoectochili roxburghii foliage fertilizer.
The method of a kind of Ecology water-surface cultivation Herba Anoectochili roxburghii the most according to claim 1, it is characterised in that described fertilizing method:
A, Herba Anoectochili roxburghii bottle seed start fertilization of leaves after planting 15 days, spray after being diluted with the water of 4 times of weight by Herba Anoectochili roxburghii foliage fertilizer
On blade face, sprayed again once every 15 days；
After B, plantation 45 days, Herba Anoectochili roxburghii foliage fertilizer is sprayed on blade face after the dilution of the water of 2 times of weight, afterwards, every
Within 15 days, spray once, until harvest.
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