CN106342527A - Greenhouse cultivation method for tissue cultured seedling of bletilla striata - Google Patents
Greenhouse cultivation method for tissue cultured seedling of bletilla striata Download PDFInfo
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- CN106342527A CN106342527A CN201610750180.4A CN201610750180A CN106342527A CN 106342527 A CN106342527 A CN 106342527A CN 201610750180 A CN201610750180 A CN 201610750180A CN 106342527 A CN106342527 A CN 106342527A
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Abstract
The invention relates to the field of agricultural product cultivation technology and specifically discloses a greenhouse cultivation method for tissue cultured seedling of bletilla striata. The method comprises the steps of land selection, soil preparation, transplanting, management and field planting. The greenhouse cultivation method adopts the 'double shed+double net' greenhouse domestication mode, which not only controls the temperature and humidity inside the greenhouse more effectively than the traditional 'single shed+single net' mode, but also maintains the temperature inside the greenhouse effectively even if the temperature outside is extremely low. In addition, the use of base material and mineral fertilizer provides a perfect soil environment for the tissue cultured seedling of bletilla striata in the process of domestication, not only effectively enhancing the survival rate of bletilla striata tissue cultured seedlingin the whole transplanting process but also shorteningthe domestication period greatly.
Description
Technical field
The present invention relates to agricultural cultivation technical field, particularly a kind of booth breeding method of Pseudobulbus Bletillae (Rhizoma Bletillae) tissue cultured seedling.
Background technology
Pseudobulbus Bletillae (Rhizoma Bletillae) is orchid in imminent danger, is traditional parts of generic medicinal plants, is Chinese Second Class Key Protected Plant kind.Due to Pseudobulbus Bletillae (Rhizoma Bletillae)
Medical value is high, consumption is big, ornamental value is high in Medicated cigarette, develop the reasons such as many in cosmetics and health product, Pseudobulbus Bletillae (Rhizoma Bletillae) needs
The amount of asking increases year by year, along with artificial xcessive digging, makes the matter worse with regard to the Pseudobulbus Bletillae (Rhizoma Bletillae) of low yield energy on original market, existing market
Upper Pseudobulbus Bletillae (Rhizoma Bletillae) supply mostlys come from the breeding of Pseudobulbus Bletillae (Rhizoma Bletillae) tuber, but because traditional tuber reproductive-cost is high, introduces a collection is few, growth cycle needs
3-4 is it is difficult to form scale, industrialization Pseudobulbus Bletillae (Rhizoma Bletillae) market.Therefore Pseudobulbus Bletillae (Rhizoma Bletillae) kind source problem has become restriction Pseudobulbus Bletillae (Rhizoma Bletillae) resource critical shortage
Bottleneck.But in recent years, manually carry out on the tissue cultured seedling surface that tissue culture obtains being to solve Pseudobulbus Bletillae (Rhizoma Bletillae) introduces a collection using Pseudobulbus Bletillae (Rhizoma Bletillae) seed
Problem, but actually the transplantation of seedlings of Pseudobulbus Bletillae (Rhizoma Bletillae) tissue culture is relatively low to survival rate in booth, and Major Difficulties are Pseudobulbus Bletillae (Rhizoma Bletillae) tissue cultured seedling in booth
Domestication in compared with unmanageable, be embodied in the control of the humidity in booth and temperature, the factor such as selection of substrate, and it tames and dociles
The change phase is also longer.Therefore need a kind of booth acclimation method of efficient Pseudobulbus Bletillae (Rhizoma Bletillae) tissue cultured seedling badly and survive come the domestication to improve Pseudobulbus Bletillae (Rhizoma Bletillae) tissue cultured seedling
Rate, shortens the domestication time.
Content of the invention
The technical problem to be solved is: for the domestic Pseudobulbus Bletillae (Rhizoma Bletillae) tissue cultured seedling booth domestication situation in Hubei, provides
A kind of booth breeding method of Pseudobulbus Bletillae (Rhizoma Bletillae) tissue cultured seedling, to improve the problem running in management during Pseudobulbus Bletillae (Rhizoma Bletillae) tissue cultured seedling is tamed in booth,
Subject matter includes the factors such as temperature in canopy, air humidity, soil moisture, the composition of substrate and proportioning, to improve Pseudobulbus Bletillae (Rhizoma Bletillae) group
The survival rate of seedlings cultivating domestication and shortening domestication time.
Above-mentioned technical purpose, the present invention is achieved in that
A kind of booth breeding method of Pseudobulbus Bletillae (Rhizoma Bletillae) tissue cultured seedling, following rapid including step:
(1) selection of land: choose the plot of loosing soil；
(2) site preparation scaffolding: carry out the previous moon turning over for the first time transplanting, after having turned over for the first time, build tunnel greenhouse,
Canopy high 3.2-3.8 rice, installs automatic water jetting pipe in booth, is turned over for the second time within first 8 days in transplanting, be furrow, furrow width 2.3-2.8
Rice, then uniformly spreads the thick substrate of one layer of 2cm on furrow, and matrix components are Masson Pine wood sawdust, sand, Vermiculitum and crushed stalk
Thing, its weight, than for 2:1:3:1, is sprayed water in stromal surface, soil moisture is maintained at 35%-45%.
(3) transplant: tissue culture interior was divided the Pseudobulbus Bletillae (Rhizoma Bletillae) transplantation of seedlings of a Seedling in seedbed, during transplanting, Pseudobulbus Bletillae (Rhizoma Bletillae) Seedling average height is
8cm, tuber average length is 1cm, builds hut, hut high 0.4-0.6 rice, width is identical with furrow width after transplanting on furrow；
(4) booth management: spray mineral micro powder fertilizer after transplanting, once a month, once spray 100g/ mu, according to transplanting
The concrete weather conditions on ground, the temperature in adjustment booth and hut and humidity in time:
A, when canopy temperature >=35 DEG C, unlimited booth and open wide hut, spread one layer of sunshade net outside booth, and little
One layer of sunshade net is spread, rate of sheltering from heat or light is 85-90% outside canopy；
B, when 35 DEG C of ＞ canopy temperature >=32 DEG C, unlimited booth and open wide hut, outside booth spread one layer of sunshade net,
Rate of sheltering from heat or light is 70-75%；
C, when 32 DEG C of ＞ canopy temperature >=25 DEG C, open wide booth but open wide hut；
D, as 25 DEG C of canopy temperature ＜, do not open wide booth and hut；
Wherein open wide the canopy film that booth/hut packs up booth/hut side wall along its length, spread outside booth/hut
If sunshade net is i.e. in the frame upper berth sunshade net of booth/hut side wall along its length；
Keep soil moisture in 30%-50%, keep when hut does not open wide air humidity in hut to be more than 85%.
(5) it is colonized: tame 3 months in booth, choose height of seedling and be more than 13cm, the transplantation of seedlings more than 1.7cm for the Tuber length
To land for growing field crops.
Compared with prior art, the invention has the advantage that
Improve the domestication survival rate of Pseudobulbus Bletillae (Rhizoma Bletillae) tissue cultured seedling, shorten the domestication time, transplant to land for growing field crops 3-4 and grow up to finished product Seedling,
Per mu yield is up to 1000kg-1300kg.
Specific embodiment
Mineral micro powder fertilizer selected by following examples is bought from Ningbo star Yao Hebairun agricultural science and technology company limited, should
Mineral micro powder trade name standing grain hundred moistens, and particle diameter is 5～25 microns.
Embodiment 1:
A kind of booth breeding method of Pseudobulbus Bletillae (Rhizoma Bletillae) tissue cultured seedling, comprises the steps:
1) selection of land: choose one block of loosing soil soil in Jingmen City, Hubei Province Jingshan County Yongxing Zhen Ting river village；
2) scaffolding site preparation: carry out the previous moon turning over for the first time transplanting, build a long 50 meters, width after turning over for the first time
12 meters, high 3.5 meters of single circular booth, install automatic water jetting pipe in booth, are turned over for the second time within first 8 days in transplanting, with
2.5 meters of a width of standards do four furrow, and then in the substrate that one layer of furrow upper berth 2cm is thick, the composition of substrate is Masson Pine wood sawdust, sand
Son, Vermiculitum and powder of straw mince, and for 2:1:3:1, the length that powder of straw minces is 1-30mm to its weight ratio, sprays water in substrate table
Face, soil moisture is maintained at 35%-45%, pinches with handss that block soil is frangible to be advisable；
(3) transplant: the Pseudobulbus Bletillae (Rhizoma Bletillae) transplantation of seedlings of a Seedling was divided in seedbed in tissue culture interior by April, 2015, Pseudobulbus Bletillae (Rhizoma Bletillae) Seedling during transplanting
Average height is 8cm, and tuber average length is 1cm, builds hut after transplanting on furrow, and the height of hut is 0.5 meter, wide by 2.5
Rice；
(4) booth management: spray mineral micro powder fertilizer after transplanting, once a month, once spray 100g/ mu, according to transplanting
The concrete weather conditions on ground, the temperature in adjustment booth and hut and humidity in time:
A, when canopy temperature >=35 DEG C, unlimited booth and open wide hut, spread one layer of sunshade net outside booth, and little
One layer of sunshade net of paving in canopy, rate of sheltering from heat or light is 90%；
B, when 35 DEG C of ＞ canopy temperature >=32 DEG C, unlimited booth both sides and open wide hut, outside booth paving one layer screening
Screened postive, rate of sheltering from heat or light is 70%；
C, when 32 DEG C of ＞ canopy temperature >=25 DEG C, open wide outside booth but open wide hut；
D, as 25 DEG C of canopy temperature ＜, do not open wide booth and hut；
Wherein open wide the canopy film that booth/hut packs up booth/hut side wall along its length, spread outside booth/hut
If sunshade net is i.e. in the frame upper berth sunshade net of booth/hut side wall along its length；
Keep soil moisture in 30%-50%, with soil handss pinch frangible and tack-free be advisable, keep when hut does not open wide
In hut, air humidity is more than 85%.
(5) it is colonized: the survival rate of in July, 2015 Pseudobulbus Bletillae (Rhizoma Bletillae) tissue cultured seedling is 83.14%, Pseudobulbus Bletillae (Rhizoma Bletillae) tissue cultured seedling average height is
15.30cm, tuber average length is 2.04cm, chooses that height of seedling is more than 13cm, Tuber length is more than the transplantation of seedlings of 1.7cm to big
Field, meet transplanting standard seedling accounting be 70.32%, transplant one month to land for growing field crops survival rate be 92.78%, transplant to
Grow up to finished product Seedling within 3 years behind land for growing field crops, per mu yield reaches 1300kg.
Control experiment
Two blocks of loosing soil soils are chosen in Jingmen City, Hubei Province Jingshan County Yongxing Zhen Ting river village in address, and the time is 2015
In July, 2015 in April.In matched group 1 and 2 not using hut, not using the substrate in embodiment 1, not using in embodiment 1
Mineral micro powder fertilizer, other domestication control measures are the same, and after matched group 1 tissue cultured seedling is tamed 3 months, survival rate is 67.22%,
Pseudobulbus Bletillae (Rhizoma Bletillae) tissue cultured seedling average height is 13.57cm, and tuber average length is 1.82cm, and the seedling accounting meeting transplanting standard is
43.47%, the survival rate transplanted month to land for growing field crops is 81.84%, control experiment 2 tame 4 months after Pseudobulbus Bletillae (Rhizoma Bletillae) tissue cultured seedling
Survival rate is 65.40%, and Pseudobulbus Bletillae (Rhizoma Bletillae) tissue cultured seedling average height is 15.11cm, and tuber average length is 1.98cm, meets transplanting standard
Seedling accounting be 67.71%, transplant one month to land for growing field crops survival rate be 83.23%.
Interpretation of result
Using " the double net of double canopies ", using unique substrate, (wood sawdust, sand, Vermiculitum and powder of straw mince what mixture formed
Substrate, its weight proportion be 2:1:3:1), spray mineral solution way to manage, Pseudobulbus Bletillae (Rhizoma Bletillae) tissue cultured seedling booth tame 3 months
Afterwards, survival rate 83.14%, survives Seedling average height 15.30cm, tuber average length 2.04cm, meets transplanting standard seedling
Accounting is 70.32%, and the survival rate to land for growing field crops one month is 92.78%.It is provided without " the double net of double canopies ", " nutrient matrix ", " ore deposit
The common management mode of thing micropowder fertilizer ", matched group 1 Pseudobulbus Bletillae (Rhizoma Bletillae) tissue cultured seedling booth tame 3 months after, survival rate 67.22%,
Survive Seedling average height up to 13.57cm, tuber average length 1.82cm, the seedling accounting meeting transplanting standard is 43.47%,
The survival rate transplanted one month to land for growing field crops is 81.84%, and matched group 2 Pseudobulbus Bletillae (Rhizoma Bletillae) tissue cultured seedling is tamed to surviving Seedling mean height in booth
Degree 15.11cm, tuber average length 1.98cm, last 4 months, and survival rate is 65.40%, meets the seedling accounting of transplanting standard
For 67.71%, the survival rate transplanted month to land for growing field crops is 83.23%.
Claims (2)
1. a kind of booth breeding method of Pseudobulbus Bletillae (Rhizoma Bletillae) tissue cultured seedling it is characterised in that: comprise the following steps:
(1) selection of land: choose the plot of loosing soil；
(2) site preparation scaffolding: carry out the previous moon turning over for the first time transplanting, build tunnel greenhouse after having turned over for the first time, canopy is high
3.2-3.8 rice, installs automatic water jetting pipe in booth, is turned over for the second time within first 8 days in transplanting, does furrow, furrow width 2.3-2.8 rice,
Then the thick substrate of one layer of 2cm is uniformly spread on furrow, matrix components are that Masson Pine wood sawdust, sand, Vermiculitum and powder of straw mince, institute
State the weight that Masson Pine wood sawdust, sand, Vermiculitum and powder of straw mince ratio for 2:1:3:1, spray water in stromal surface, soil moisture is protected
Hold in 35%-45%；
(3) transplant: the Pseudobulbus Bletillae (Rhizoma Bletillae) transplantation of seedlings of a Seedling was divided in seedbed in tissue culture interior, during transplanting, Pseudobulbus Bletillae (Rhizoma Bletillae) Seedling average height is 8cm,
Tuber average length is 1cm, builds hut, hut high 0.4-0.6 rice, width are identical with furrow width after transplanting on furrow；
(4) booth management: spray mineral micro powder fertilizer after transplanting, once a month, once spray 100g/ mu, according to transplanting ground
Concrete weather conditions, the temperature in adjustment booth and hut and humidity in time, keep soil moisture in 30%-50%, when hut not
Air humidity in hut is kept to be more than 85% when unlimited；
(5) it is colonized: tame 3 months in booth, choose height of seedling and be more than 13cm, the domestication transplantation of seedlings more than 1.7cm for the Tuber length
To land for growing field crops.
2. Pseudobulbus Bletillae (Rhizoma Bletillae) tissue cultured seedling according to claim 1 booth breeding method it is characterised in that in described step (4) and
When adjustment booth and hut in temperature and the method for humidity include:
A, when canopy temperature >=35 DEG C, unlimited booth and open wide hut, spread one layer of sunshade net outside booth, and outside hut
Side spreads one layer of sunshade net, and rate of sheltering from heat or light is 85-90%；
B, when 35 DEG C of ＞ canopy temperature >=32 DEG C, unlimited booth and open wide hut, outside booth spread one layer of sunshade net, shelter from heat or light
Rate is 70-75%；
C, when 32 DEG C of ＞ canopy temperature >=25 DEG C, open wide booth but open wide hut；
D, as 25 DEG C of canopy temperature ＜, do not open wide booth and hut.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106922370A (en) * | 2017-04-25 | 2017-07-07 | 江苏农林职业技术学院 | A kind of cultural method for promoting bletilla striata pseudobulb to expand |
| CN114051868A (en) * | 2021-12-21 | 2022-02-18 | 上海辰山植物园 | Rapid cultivation method of rhizoma bletillae tissue culture seedlings |
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| CN103314854A (en) * | 2013-07-08 | 2013-09-25 | 重庆市秀山红星中药材开发有限公司 | Domestication cultivation method of bletilla tissue culture seedlings |
| CN103766115A (en) * | 2014-01-07 | 2014-05-07 | 陕西师范大学 | Method for transplanting bletilla striata seedlings |
| CN104604497A (en) * | 2015-01-28 | 2015-05-13 | 李泽诚 | Bletilla striata tissue culture seedling exercising and cultivation method |
| CN104782375A (en) * | 2015-04-23 | 2015-07-22 | 陕西师范大学 | Germination accelerating method for Bletilla ochracea corm in winter |
| CN205431151U (en) * | 2016-02-02 | 2016-08-10 | 云南万盛食用菌种植有限公司 | Greenhouse is cultivateed to domestic fungus |
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2016
- 2016-08-27 CN CN201610750180.4A patent/CN106342527A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103314854A (en) * | 2013-07-08 | 2013-09-25 | 重庆市秀山红星中药材开发有限公司 | Domestication cultivation method of bletilla tissue culture seedlings |
| CN103766115A (en) * | 2014-01-07 | 2014-05-07 | 陕西师范大学 | Method for transplanting bletilla striata seedlings |
| CN104604497A (en) * | 2015-01-28 | 2015-05-13 | 李泽诚 | Bletilla striata tissue culture seedling exercising and cultivation method |
| CN104782375A (en) * | 2015-04-23 | 2015-07-22 | 陕西师范大学 | Germination accelerating method for Bletilla ochracea corm in winter |
| CN205431151U (en) * | 2016-02-02 | 2016-08-10 | 云南万盛食用菌种植有限公司 | Greenhouse is cultivateed to domestic fungus |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106922370A (en) * | 2017-04-25 | 2017-07-07 | 江苏农林职业技术学院 | A kind of cultural method for promoting bletilla striata pseudobulb to expand |
| CN114051868A (en) * | 2021-12-21 | 2022-02-18 | 上海辰山植物园 | Rapid cultivation method of rhizoma bletillae tissue culture seedlings |
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| TA01 | Transfer of patent application right |
Effective date of registration: 20190516 Address after: 430051 Medical Health Park, Hanyang District, Wuhan City, Hubei Province Applicant after: Kyushu Tianrun (Wuhan) Research Institute of Chinese medicine limited company Address before: 442101 Qingfeng Village Group 13, Fangxian Qingfeng Town, Shiyan City, Hubei Province Applicant before: Fangxian Jiuzhou medicinal plant science and Technology Co Ltd |
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Application publication date: 20170125 |
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