CN103314854A - Domestication cultivation method of bletilla tissue culture seedlings - Google Patents
Domestication cultivation method of bletilla tissue culture seedlings Download PDFInfo
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Abstract
The invention discloses a domestication cultivation method of bletilla tissue culture seedlings, which belongs to the technical field of Chinese herbal medicine planting. The method comprises the following five steps: matrix preparation, hardening-seedling, bottling-out, domestication cultivation, and domestication management. The method has the beneficial effects that a method for transiting bletilla tissue culture seedlings from an experiment bottle to the nature is provided, so that the absorption function of root hairs, the guided transport function of stems and the photosynthesis function of leaves of tissue culture seedlings can be quickly improved, and therefore, the tissue culture seedlings can adapt to the natural environment quickly, the transplanting survival rate of tissue culture seedlings is improved, and by using the method, the transplanting survival rate of domestication seedlings reaches over 92%.
Description
Technical field
The invention belongs to traditional Chinese medicine planting technology field, particularly a kind of domesticating cultivation method of bletilla striata group training seedling.
Background technology
The bletilla striata is that the orchid family bletilla striata belongs to herbaceos perennial, calls white root, Pseudobulbus Bletillae (Rhizoma Bletillae), Cold boiled chicken baby, YANGJIAOQI, purple orchid, triflorous ainsliaea leaf, company and grass etc.With the stem tuber hyoscine, energy astringing to arrest bleeding, detumescence and promoting granulation; Control the pulmonary tuberculosis hemoptysis, bronchiectasis hemoptysis, stomach ulcer haematemesis, hematuria, the disease such as have blood in stool.The diseases such as traumatism and bleeding, burn and scald, rhagadia manus et pedis are controlled in external application.There is huge commercial exploitation to be worth at aspects such as biological medicine, health food, textile printing and dyeing, sapecial coating and daily-use chemical industries.Along with the demand of the development and use of bletilla striata food and dyeing industry, beauty culture, bletilla striata consumption increases increasingly.
The seed nature germination rate of the bletilla striata is extremely low, the breeding difficulty, and wild resource is rare, because demand is large, wild resource frequently faces exhaustion, now lists " animals and plants kind international trade pact in imminent danger " in and is protected, and therefore only takes artificial breeding.
Present conventional method is main mainly with plant division greatly, is about to the stem tuber plantation that is cut into small pieces, and reproduction coefficient is low, can't the satisfying the market needs.People have begun artificial propagation, and many places have adopted tissue culture propagation method with a large amount of seedling of quick acquisition; Because tissue culture propagation carries out in artificial environment, its oneself supports poor ability, particularly the photosynthesis function of dredging transportation function, blade of the absorption function of its coring, cane can not be gathered the right extraneous environment that adapts to, can't bring into play its due function fully, cause like this transplanting survival rate very low, therefore need to tame and to transplant smoothly, to improve its survival rate.
Summary of the invention
Technical problem to be solved by this invention is: a kind of domesticating method that increases bletilla striata group training transplantation of seedlings success rate is provided, and better reform of nature circle environment makes the institutional framework of plant more substantial, with the success of assurance group training seedling replanting.
For solving the problems of the technologies described above, the technical solution adopted in the present invention is a kind of domesticating cultivation method of bletilla striata group training seedling, comprises substrate preparation, and hardening, bottle outlet, domesticating and cultivating, domestication management five large steps is characterized in that concrete steps are as follows.
⑴ substrate preparation: mix with 0.5 part of Nitrogen, Phosphorus and Potassium after with 1 part of perlite, 3 parts of peat soils, 1 part of pulverizing of silica according to weight portion and to do that to train in groups seedling domestication matrix for subsequent use; The N-P-K content of described Nitrogen, Phosphorus and Potassium is N:P:K=10:10:10.
⑵ hardening: plant height 3-4cm, the coring group training seedling more than 5 is moved to the hardening room together with the seedling bottle carry out hardening, under indoor natural light, placed 4-5 days, again bottle cap is opened, placed 2-3 days, allow air free come in and go out.
⑶ bottle outlet: in the seedling bottle, inject a small amount of running water, take out gently together with medium with tweezers, put into the clear water basin and clean medium, then wash gently with running water.
⑷ domesticating and cultivating: the group training transplantation of seedlings that will rinse well to the bletilla striata group in the booth is trained on the special-purpose rooting culture bed of seedling, at first in the thick group training seedling domestication matrix of husky bed tiling 4-6cm, and spray 3 ‰ liquor potassic permanganate and carry out disinfection, then will organize the training transplantation of seedlings on matrix, every pocket 3 strains, its distance between rows and hills is 12cm * 15cm, dig the alveole of 5-6cm with finger, to organize the training shoot root must put into and build with matrix afterwards, without compacting, then spray clear water, keep water content of substrate in the scope of 65%-70%.
⑸ tame management: will tame the interior temperature of canopy and be controlled between 18 ℃-28 ℃, spray 4-6 every day, and each 3-5min is controlled in the scope of 65%-70% water content of substrate, air humidity is controlled in the 88%-92% scope, and illuminance is controlled in the scope of 3000lx-3500lx; Transplant and sprayed 1 ‰ kalium nitrate fertilizers in rear 10 days, spray one time 5 ‰ Nitrogen, Phosphorus and Potassium every two weeks later; After 80-90 days, obtain coring and reach 7-10cm, the domestication seedling of ground plant height more than 7-10cm.
The special-purpose rooting culture bed of the described bletilla striata group training seedling defending party to the application is applied for a patent, and number of patent application is: 2013203813870
The invention has the beneficial effects as follows that training seedling for bletilla striata group provides a suitable transition method from the test bottle to the Nature, make the very enough absorption function of its coring, the photosynthesis functions of dredging transportation function, blade of cane improved very soon of group training seedling, reform of nature environment quickly, the transplanting survival rate of raising group training seedling, use the method, domestication transplantation of seedlings survival rate is reached more than 92%.
Embodiment
The invention will be further described below in conjunction with embodiment, and following examples are intended to illustrate the present invention rather than limitation of the invention further, should not limit protection scope of the present invention with this.
Embodiment 1.
Preparation domestication matrix at first with 1 ton of perlite, 3 tons of peat soils, 1 ton of particle that is ground into respectively below the particle diameter 2mm of silica, is that 0.5 ton of the Nitrogen, Phosphorus and Potassium of 10:10:10 mixes with N-P-K content then, namely does and trains in groups seedling domestication matrix.
Plant height 3-4cm, the coring group training seedling more than 5 is moved to the hardening room together with the seedling bottle carry out hardening, under indoor natural light, place after 4 days, again bottle cap is opened, placed 2 days, allow air free come in and go out; Then in the seedling bottle, inject a small amount of running water, take out gently together with medium with tweezers, put into the clear water basin and clean medium, then wash gently with running water; The group training transplantation of seedlings that to rinse well again to the bletilla striata group in the booth is trained on the special-purpose rooting culture bed of seedling, at first in the thick group training seedling domestication matrix of husky bed tiling 4cm, and spray 3 ‰ liquor potassic permanganate and carry out disinfection, then will organize the training transplantation of seedlings on matrix, every pocket 3 strains, its distance between rows and hills is 12cm * 15cm, dig the alveole of 5-6cm with finger, 1000 pockets have been cultivated altogether, to organize the training shoot root during cultivation must put into and build with matrix afterwards, without compacting, then spray clear water, keep water content of substrate in the scope of 65%-70%; To tame at ordinary times the interior temperature of canopy and be controlled between 18 ℃-28 ℃, when temperature is 18 ℃, spray every day 4 times, each 3min, when temperature is 23 ℃, spray each 4min every day 5 times, when temperature is 28 ℃, spray every day 6 times, each 5min is controlled in the scope of 65%-70% water content of substrate, air humidity is controlled in the 88%-92% scope, and illuminance is controlled in the scope of 3000lx-3500lx; Transplant and sprayed 1 ‰ kalium nitrate fertilizers in rear 10 days, spray one time 5 ‰ Nitrogen, Phosphorus and Potassium every two weeks later; After 80 days, obtain coring and reach 7cm, the domestication seedling of ground plant height more than 7cm, 1000 pockets all survive, and wherein have 68 pockets only to survive 1 strain, and 101 pockets have survived 2 strains, and remaining every pocket 3 strain all survives, and survival rate reaches 92.1%.
Embodiment 2.
Preparation domestication matrix at first with 1 ton of perlite, 3 tons of peat soils, 1 ton of particle that is ground into respectively below the particle diameter 2mm of silica, is that 0.5 ton of the Nitrogen, Phosphorus and Potassium of 10:10:10 mixes with N-P-K content then, namely does and trains in groups seedling domestication matrix.
Plant height 3-4cm, the coring group training seedling more than 5 is moved to the hardening room together with the seedling bottle carry out hardening, under indoor natural light, place after 4.5 days, again bottle cap is opened, placed 2 days half, allow air free come in and go out; Then in the seedling bottle, inject a small amount of running water, take out gently together with medium with tweezers, put into the clear water basin and clean medium, then wash gently with running water; The group training transplantation of seedlings that to rinse well again to the bletilla striata group in the booth is trained on the special-purpose rooting culture bed of seedling, at first in the thick group training seedling domestication matrix of husky bed tiling 5cm, and spray 3 ‰ liquor potassic permanganate and carry out disinfection, then will organize the training transplantation of seedlings on matrix, every pocket 3 strains, its distance between rows and hills is 12cm * 15cm, dig the alveole of 5-6cm with finger, 1000 pockets have been cultivated altogether, to organize the training shoot root during cultivation must put into and build with matrix afterwards, without compacting, then spray clear water, keep water content of substrate in the scope of 65%-70%; To tame at ordinary times the interior temperature of canopy and be controlled between 18 ℃-28 ℃, when temperature is 18 ℃, spray every day 4 times, each 3min, when temperature is 23 ℃, spray each 4min every day 5 times, when temperature is 28 ℃, spray every day 6 times, each 5min is controlled in the scope of 65%-70% water content of substrate, air humidity is controlled in the 88%-92% scope, and illuminance is controlled in the scope of 3000lx-3500lx; Transplant and sprayed 1 ‰ kalium nitrate fertilizers in rear 10 days, spray one time 5 ‰ Nitrogen, Phosphorus and Potassium every two weeks later; After 85 days, obtain coring and reach 8cm, the domestication seedling of ground plant height more than 8cm, 1000 pockets all survive, and wherein have 51 pockets only to survive 1 strain, and 89 pockets have survived 2 strains, and remaining 3 strain all survives, and survival rate reaches 93.7%.
Embodiment 3.
Preparation domestication matrix at first with 1 ton of perlite, 3 tons of peat soils, 1 ton of particle that is ground into respectively below the particle diameter 2mm of silica, is that 0.5 ton of the Nitrogen, Phosphorus and Potassium of 10:10:10 mixes with N-P-K content then, namely does and trains in groups seedling domestication matrix.
Plant height 3-4cm, the coring group training seedling more than 5 is moved to the hardening room together with the seedling bottle carry out hardening, under indoor natural light, place after 5 days, again bottle cap is opened, placed 3 days, allow air free come in and go out; Then in the seedling bottle, inject a small amount of running water, take out gently together with medium with tweezers, put into the clear water basin and clean medium, then wash gently with running water; The group training transplantation of seedlings that to rinse well again to the bletilla striata group in the booth is trained on the special-purpose rooting culture bed of seedling, at first in the thick group training seedling domestication matrix of husky bed tiling 6cm, and spray 3 ‰ liquor potassic permanganate and carry out disinfection, then will organize the training transplantation of seedlings on matrix, every pocket 3 strains, its distance between rows and hills is 12cm * 15cm, dig the alveole of 5-6cm with finger, 1000 pockets have been cultivated altogether, to organize the training shoot root during cultivation must put into and build with matrix afterwards, without compacting, then spray clear water, keep water content of substrate in the scope of 65%-70%; To tame at ordinary times the interior temperature of canopy and be controlled between 18 ℃-28 ℃, when temperature is 18 ℃, spray every day 4 times, each 3min, when temperature is 23 ℃, spray each 4min every day 5 times, when temperature is 28 ℃, spray every day 6 times, each 5min is controlled in the scope of 65%-70% water content of substrate, air humidity is controlled in the 88%-92% scope, and illuminance is controlled in the scope of 3000lx-3500lx; Transplant and sprayed 1 ‰ kalium nitrate fertilizers in rear 10 days, spray one time 5 ‰ Nitrogen, Phosphorus and Potassium every two weeks later; After 90 days, obtain coring and reach 10cm, the domestication seedling of ground plant height more than 10cm, 1000 pockets all survive, and wherein have 50 pockets only to survive 1 strain, and 113 pockets have survived 2 strains, and remaining 3 strain all survives, and survival rate reaches 92.9%.
Claims (1)
1. the domesticating cultivation method of bletilla striata group training seedling comprises substrate preparation, and hardening, bottle outlet, domesticating and cultivating, domestication management five large steps is characterized in that concrete steps are as follows:
⑴ substrate preparation: mix with 0.5 part of Nitrogen, Phosphorus and Potassium after with 1 part of perlite, 3 parts of peat soils, 1 part of pulverizing of silica according to weight portion and to do that to train in groups seedling domestication matrix for subsequent use; The N-P-K content of described Nitrogen, Phosphorus and Potassium is N:P:K=10:10:10;
⑵ hardening: plant height 3-4cm, the coring group training seedling more than 5 is moved to the hardening room together with the seedling bottle carry out hardening, under indoor natural light, placed 4-5 days, again bottle cap is opened, placed 2-3 days;
⑶ bottle outlet: in the seedling bottle, inject a small amount of running water, take out gently together with medium with tweezers, put into the clear water basin and clean medium, then wash gently with running water;
⑷ domesticating and cultivating: the group training transplantation of seedlings that will rinse well to the bletilla striata group in the booth is trained on the special-purpose rooting culture bed of seedling, at first in the thick group training seedling domestication matrix of husky bed tiling 4-6cm, and spray 3 ‰ liquor potassic permanganate and carry out disinfection, then will organize the training transplantation of seedlings on matrix, every pocket 3 strains, its distance between rows and hills is 12cm * 15cm, dig the alveole of 5-6cm with finger, to organize the training shoot root must put into and build with matrix afterwards, without compacting, then spray clear water, keep water content of substrate in the scope of 65%-70%;
⑸ tame management: will tame the interior temperature of canopy and be controlled between 18 ℃-28 ℃, spray 4-6 every day, and each 3-5min is controlled in the scope of 65%-70% water content of substrate, air humidity is controlled in the 88%-92% scope, and illuminance is controlled in the scope of 3000lx-3500lx; Transplant and sprayed 1 ‰ kalium nitrate fertilizers in rear 10 days, spray one time 5 ‰ Nitrogen, Phosphorus and Potassium every two weeks later; After 80-90 days, obtain coring and reach 7-10cm, the ground plant height is at the domestication seedling of 7-10cm.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104604497A (en) * | 2015-01-28 | 2015-05-13 | 李泽诚 | Bletilla striata tissue culture seedling exercising and cultivation method |
| CN104719152A (en) * | 2015-02-16 | 2015-06-24 | 贵州省农作物品种资源研究所 | Rhizoma bletillae industrialized seedling method |
| CN104938170A (en) * | 2015-05-27 | 2015-09-30 | 贵州省农作物品种资源研究所 | Greenhouse cultivation method for rhizoma bletillae seed stems |
| CN104957019A (en) * | 2015-07-29 | 2015-10-07 | 四川农业大学 | Seedling strengthening nutrient solution for bletilla tissue culture seedling, nutrient composition and seedling strengthening method |
| CN105103928A (en) * | 2015-09-29 | 2015-12-02 | 江苏农林职业技术学院 | Method for acclimatizing and transplanting bletilla striata tissue culture seedlings |
| CN105165360A (en) * | 2015-09-29 | 2015-12-23 | 江苏农林职业技术学院 | Bletilla striata cultivation method |
| CN106069780A (en) * | 2016-07-29 | 2016-11-09 | 衢州康源生物科技有限公司 | A kind of technology using Pseudobulbus Bletillae (Rhizoma Bletillae) tuber to carry out group cultivation kind |
| CN106305055A (en) * | 2016-08-18 | 2017-01-11 | 安徽省彩枫农林科技有限公司 | Domestication method for rhizoma bletillae seedlings |
| CN106305056A (en) * | 2016-08-18 | 2017-01-11 | 安徽省彩枫农林科技有限公司 | Method for accelerating growth of rhizoma bletillae domestication seedlings to form adult seedlings |
| CN106342527A (en) * | 2016-08-27 | 2017-01-25 | 房县九州通药用植物科技有限公司 | Greenhouse cultivation method for tissue cultured seedling of bletilla striata |
| CN106417027A (en) * | 2016-10-14 | 2017-02-22 | 贵州万重山生态农业开发有限公司 | Low-temperature seedling hardening method for bletilla striata tissue culture seedlings |
| CN106577002A (en) * | 2016-12-23 | 2017-04-26 | 江苏农林职业技术学院 | Bletilla striata culture medium, and preparation method and application thereof |
| CN106688848A (en) * | 2016-11-29 | 2017-05-24 | 云南省农业科学院药用植物研究所 | Efficient exercising technique for Bletilla striata tissue culture seedlings |
| CN106718721A (en) * | 2016-11-24 | 2017-05-31 | 宣威市农硕农特产品开发有限公司 | A kind of domesticating cultivation method of bletilla tissue culture seedlings |
| CN107548914A (en) * | 2017-09-29 | 2018-01-09 | 道真自治县百草谷旅游和农业开发有限责任公司 | A kind of implantation methods of the bletilla striata |
| CN107864865A (en) * | 2017-12-26 | 2018-04-03 | 丽江市古城区秋成种养殖有限公司 | A kind of efficient hardening technology and domesticating cultivation method of haw ginseng tissue-cultured seedling |
| CN108770623A (en) * | 2018-06-23 | 2018-11-09 | 云南省农业科学院农业环境资源研究所 | The overwintering no suspend mode hardening off method of bletilla tissue culture seedlings duplicature |
| CN108901720A (en) * | 2018-06-23 | 2018-11-30 | 云南省农业科学院农业环境资源研究所 | Bletilla tissue culture seedlings waveform seedling bed hardening off method |
| CN109349077A (en) * | 2018-09-26 | 2019-02-19 | 河南云帮农业科技有限公司 | A kind of bletilla striata tissue-culture container seedling domestication transplantation method |
| CN114667857A (en) * | 2022-02-08 | 2022-06-28 | 贵州省农作物品种资源研究所 | Bletilla striata seedling domestication optimization method and domesticated bletilla striata seedlings |
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2013
- 2013-07-08 CN CN 201310282938 patent/CN103314854A/en active Pending
Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104604497A (en) * | 2015-01-28 | 2015-05-13 | 李泽诚 | Bletilla striata tissue culture seedling exercising and cultivation method |
| CN104719152A (en) * | 2015-02-16 | 2015-06-24 | 贵州省农作物品种资源研究所 | Rhizoma bletillae industrialized seedling method |
| CN104938170A (en) * | 2015-05-27 | 2015-09-30 | 贵州省农作物品种资源研究所 | Greenhouse cultivation method for rhizoma bletillae seed stems |
| CN104957019A (en) * | 2015-07-29 | 2015-10-07 | 四川农业大学 | Seedling strengthening nutrient solution for bletilla tissue culture seedling, nutrient composition and seedling strengthening method |
| CN105103928A (en) * | 2015-09-29 | 2015-12-02 | 江苏农林职业技术学院 | Method for acclimatizing and transplanting bletilla striata tissue culture seedlings |
| CN105165360A (en) * | 2015-09-29 | 2015-12-23 | 江苏农林职业技术学院 | Bletilla striata cultivation method |
| CN106069780B (en) * | 2016-07-29 | 2018-07-20 | 衢州康源生物科技有限公司 | A technique for tissue culture breeding is carried out using bletilla stem tuber |
| CN106069780A (en) * | 2016-07-29 | 2016-11-09 | 衢州康源生物科技有限公司 | A kind of technology using Pseudobulbus Bletillae (Rhizoma Bletillae) tuber to carry out group cultivation kind |
| CN106305055A (en) * | 2016-08-18 | 2017-01-11 | 安徽省彩枫农林科技有限公司 | Domestication method for rhizoma bletillae seedlings |
| CN106305056A (en) * | 2016-08-18 | 2017-01-11 | 安徽省彩枫农林科技有限公司 | Method for accelerating growth of rhizoma bletillae domestication seedlings to form adult seedlings |
| CN106342527A (en) * | 2016-08-27 | 2017-01-25 | 房县九州通药用植物科技有限公司 | Greenhouse cultivation method for tissue cultured seedling of bletilla striata |
| CN106417027A (en) * | 2016-10-14 | 2017-02-22 | 贵州万重山生态农业开发有限公司 | Low-temperature seedling hardening method for bletilla striata tissue culture seedlings |
| CN106718721A (en) * | 2016-11-24 | 2017-05-31 | 宣威市农硕农特产品开发有限公司 | A kind of domesticating cultivation method of bletilla tissue culture seedlings |
| CN106688848A (en) * | 2016-11-29 | 2017-05-24 | 云南省农业科学院药用植物研究所 | Efficient exercising technique for Bletilla striata tissue culture seedlings |
| CN106688848B (en) * | 2016-11-29 | 2019-11-26 | 云南省农业科学院药用植物研究所 | A kind of efficient hardening technology of bletilla tissue-cultured seedling |
| CN106577002A (en) * | 2016-12-23 | 2017-04-26 | 江苏农林职业技术学院 | Bletilla striata culture medium, and preparation method and application thereof |
| CN107548914A (en) * | 2017-09-29 | 2018-01-09 | 道真自治县百草谷旅游和农业开发有限责任公司 | A kind of implantation methods of the bletilla striata |
| CN107864865A (en) * | 2017-12-26 | 2018-04-03 | 丽江市古城区秋成种养殖有限公司 | A kind of efficient hardening technology and domesticating cultivation method of haw ginseng tissue-cultured seedling |
| CN108770623A (en) * | 2018-06-23 | 2018-11-09 | 云南省农业科学院农业环境资源研究所 | The overwintering no suspend mode hardening off method of bletilla tissue culture seedlings duplicature |
| CN108901720A (en) * | 2018-06-23 | 2018-11-30 | 云南省农业科学院农业环境资源研究所 | Bletilla tissue culture seedlings waveform seedling bed hardening off method |
| CN109349077A (en) * | 2018-09-26 | 2019-02-19 | 河南云帮农业科技有限公司 | A kind of bletilla striata tissue-culture container seedling domestication transplantation method |
| CN114667857A (en) * | 2022-02-08 | 2022-06-28 | 贵州省农作物品种资源研究所 | Bletilla striata seedling domestication optimization method and domesticated bletilla striata seedlings |
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Application publication date: 20130925 |