CN106069780A - A kind of technology using Pseudobulbus Bletillae (Rhizoma Bletillae) tuber to carry out group cultivation kind - Google Patents
A kind of technology using Pseudobulbus Bletillae (Rhizoma Bletillae) tuber to carry out group cultivation kind Download PDFInfo
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- CN106069780A CN106069780A CN201610617266.XA CN201610617266A CN106069780A CN 106069780 A CN106069780 A CN 106069780A CN 201610617266 A CN201610617266 A CN 201610617266A CN 106069780 A CN106069780 A CN 106069780A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The present invention discloses a kind of technology using Pseudobulbus Bletillae (Rhizoma Bletillae) tuber to carry out group cultivation kind, including environment disinfected, substrate preparation, in-bottle seeding, bottle outlet, sow and cultivate be cultivate, substrate is prepared by sandy soil or folder sandy soil, fertile soil and wood sawdust mix homogeneously, and clean tissue cultured seedling is one clump with 3 by the transplanting method of Pseudobulbus Bletillae (Rhizoma Bletillae) tissue cultured seedling, by the mode surface planting of row Herba Alii fistulosi to seedbed；This employing Pseudobulbus Bletillae (Rhizoma Bletillae) tuber carries out the technology that group cultivates kind can be effectively increased Pseudobulbus Bletillae (Rhizoma Bletillae) tissue cultured seedling to the survival rate in land for growing field crops and yield, makes transplantation of seedlings survival rate to more than 95%；There is the advantage that cost of investment is low simultaneously.
Description
Technical field
Present invention relates particularly to a kind of technology using Pseudobulbus Bletillae (Rhizoma Bletillae) tuber to carry out group cultivation kind.
Background technology
Pseudobulbus Bletillae (Rhizoma Bletillae) is perennial herb.Pseudobulb tuber shape, white, plump, there is finger-like difference.Spend beautiful greatly, aubergine,
It is available for cultivation to view and admire.Pseudobulb, containing compositions such as Rhizoma Bletillae gel, starch, volatile oil, has the effects such as convergence, tonifying the lung hemostasis, detumescence, can be interior
Take external application, be applied to the diseases such as hemoptysis haematemesis, traumatic hemorrhage, sore swollen toxin, chapped skin, pulmonary tuberculosis hemoptysis, ulcer haemorrhage.In vain
And be a kind of wild plant the most in imminent danger, the condition of seed germination is harsh, needs and mycosymbiosis, adds that Seedling Stage is longer, right
Environment sensitive, is therefore difficult to effectively be bred by seed under natural environment situation.Along with the exhaustion of wild resource, in vain
And medical material market price rising all the way, break through 400 yuan/kg high point.Under the stimulation of so high price, the wild white of last remaining
And resource is anticipated will exhaust in the near future.Under the ordering about of interests, plantation family is sought for Pseudobulbus Bletillae (Rhizoma Bletillae) artificial culture, but due to
Seedling is the deficientest, is difficult to scale development, even can not start to walk.
Summary of the invention
The technical problem to be solved in the present invention is to provide and a kind of can be effectively increased the survival to land for growing field crops of the Pseudobulbus Bletillae (Rhizoma Bletillae) tissue cultured seedling
Rate and yield, make transplantation of seedlings survival rate to more than 95%；The employing Pseudobulbus Bletillae (Rhizoma Bletillae) tuber simultaneously having cost of investment low carries out group
Cultivate the technology planted.
For solving the problems referred to above, the present invention adopts the following technical scheme that
A kind of technology using Pseudobulbus Bletillae (Rhizoma Bletillae) tuber to carry out group cultivation kind, including environment disinfected, substrate preparation, in-bottle seeding, goes out
Bottle, sow and cultivate be cultivate.
The described technology using Pseudobulbus Bletillae (Rhizoma Bletillae) tuber to carry out group cultivation kind, comprises the following steps:
Environment disinfected: by group training operating room, culturing room's sterilization, then HD-850 type table above formula horizontal blast cleaning is worked
Platform carries out disinfection in being placed on group training operating room, it is ensured that workbench is in gnotobasis in working area；
Substrate preparation: sandy soil or folder sandy soil, fertile soil and wood sawdust mix homogeneously are made tissue cultured seedling substrate, standby
With；
In-bottle seeding: take Pseudobulbus Bletillae (Rhizoma Bletillae) tuber, through sterilization treatment, as the outer implant of detoxication and tissue culture, by be cultivated white
And tissue cultured seedling move to booth together with Seedling bottle in carry out seedling exercising, place 10-15 days under indoor natural light, then bottle cap opened, put
Put 1 day；
Bottle outlet: have the tweezers of Cotton Gossypii take out tissue cultured seedling and the culture medium of its root cleaned by head bundle, makes tissue cultured seedling to the greatest extent
Individual plant may be divided into；Cleaned tissue cultured seedling is put into ventilating and cooling place and places a few hours, then cover tissue cultured seedling with wet towel
Blade, and only root is exposed, finally root is placed in carbendazim aqueous solution immersion several minutes, transfers to root-growing agent water-soluble
Liquid soaks several minutes；
Booth seedling exercising is trained: is taken out from culture bottle by seedling, with 800-1000 times of carbendazol soaking and washing 2-3 time, general
The culture medium of root is washed off, in then compost Seedling dish is arrived in plantation, the Seedling dish having planted Pseudobulbus Bletillae (Rhizoma Bletillae) Seedling is put into experienced Seedling booth and cultivates
2-3 month, use tidal irrigation, and periodically spray foliage fertilizer；
Seedling is practiced in sylvan life: removes after cultivating 2-3 month in booth in booth takes out of the woods and cultivates two months；
Transplant: be implanted into soil after two months through sylvan life cultivation and naturally grow.
The invention has the beneficial effects as follows: owing to the component of tissue cultured seedling substrate is by sandy soil or folder sandy soil, fertile soil and saw
Wood flour forms so that soil permeability is good, the most beneficially the growth of Pseudobulbus Bletillae (Rhizoma Bletillae) tissue cultured seedling；Transplanting method by clean tissue cultured seedling with 3
It it is one clump, by the mode surface planting of row Herba Alii fistulosi to seedbed, it is possible to be effectively increased tissue cultured seedling survival rate；There is provided simultaneously one applicable
Transition method from test bottle to the Nature, make tissue cultured seedling the most enough quickly improve the absorption function of its root hair, cane dredge fortune
Transmission function, the photosynthesis function of blade, can reform of nature environment quickly, improve the transplanting survival rate of tissue cultured seedling.
Detailed description of the invention
Embodiment one:
A kind of technology using Pseudobulbus Bletillae (Rhizoma Bletillae) tuber to carry out group cultivation kind, including environment disinfected, substrate preparation, in-bottle seeding, goes out
Bottle, sow and cultivate be cultivate.
The described technology using Pseudobulbus Bletillae (Rhizoma Bletillae) tuber to carry out group cultivation kind, comprises the following steps:
Environment disinfected: by group training operating room, culturing room's sterilization, then HD-850 type table above formula horizontal blast cleaning is worked
Platform carries out disinfection in being placed on group training operating room, it is ensured that workbench is in gnotobasis in working area；
Substrate preparation: sandy soil or folder sandy soil, fertile soil and wood sawdust are done by proportion 3:2:1 mix homogeneously and trains in groups
Seedling substrate, standby；
In-bottle seeding: take Pseudobulbus Bletillae (Rhizoma Bletillae) tuber, through sterilization treatment, as the outer implant of detoxication and tissue culture, by be cultivated white
And tissue cultured seedling move to booth together with Seedling bottle in carry out seedling exercising, place 10-15 days under indoor natural light, then bottle cap opened, put
Put 1 day；
Bottle outlet: have the tweezers of Cotton Gossypii take out tissue cultured seedling and the culture medium of its root cleaned by head bundle, makes tissue cultured seedling to the greatest extent
Individual plant may be divided into；Cleaned tissue cultured seedling is put into ventilating and cooling place and places a few hours, then cover tissue cultured seedling with wet towel
Blade, and only root is exposed, finally root is placed in carbendazim aqueous solution immersion several minutes, transfers to root-growing agent water-soluble
Liquid soaks several minutes；
Booth seedling exercising is trained: is taken out from culture bottle by seedling, with 800-1000 times of carbendazol soaking and washing 2-3 time, general
The culture medium of root is washed off, in then compost Seedling dish is arrived in plantation, the Seedling dish having planted Pseudobulbus Bletillae (Rhizoma Bletillae) Seedling is put into experienced Seedling booth and cultivates
2-3 month, use tidal irrigation, and periodically spray foliage fertilizer；
Seedling is practiced in sylvan life: removes after cultivating 2-3 month in booth in booth takes out of the woods and cultivates two months；
Transplant: be implanted into soil after two months through sylvan life cultivation and naturally grow.
Embodiment two:
A kind of technology using Pseudobulbus Bletillae (Rhizoma Bletillae) tuber to carry out group cultivation kind, including environment disinfected, substrate preparation, in-bottle seeding, goes out
Bottle, sow and cultivate be cultivate.
The described technology using Pseudobulbus Bletillae (Rhizoma Bletillae) tuber to carry out group cultivation kind, comprises the following steps:
Environment disinfected: by group training operating room, culturing room's sterilization, then HD-850 type table above formula horizontal blast cleaning is worked
Platform carries out disinfection in being placed on group training operating room, it is ensured that workbench is in gnotobasis in working area；
Substrate preparation: sandy soil or folder sandy soil, fertile soil and wood sawdust are done by proportion 1:1:1 mix homogeneously and trains in groups
Seedling substrate, standby；
In-bottle seeding: take Pseudobulbus Bletillae (Rhizoma Bletillae) tuber, through sterilization treatment, as the outer implant of detoxication and tissue culture, by be cultivated white
And tissue cultured seedling move to booth together with Seedling bottle in carry out seedling exercising, place 10-15 days under indoor natural light, then bottle cap opened, put
Put 1 day；
Bottle outlet: have the tweezers of Cotton Gossypii take out tissue cultured seedling and the culture medium of its root cleaned by head bundle, makes tissue cultured seedling to the greatest extent
Individual plant may be divided into；Cleaned tissue cultured seedling is put into ventilating and cooling place and places a few hours, then cover tissue cultured seedling with wet towel
Blade, and only root is exposed, finally root is placed in carbendazim aqueous solution immersion several minutes, transfers to root-growing agent water-soluble
Liquid soaks several minutes；
Booth seedling exercising is trained: is taken out from culture bottle by seedling, with 800-1000 times of carbendazol soaking and washing 2-3 time, general
The culture medium of root is washed off, in then compost Seedling dish is arrived in plantation, the Seedling dish having planted Pseudobulbus Bletillae (Rhizoma Bletillae) Seedling is put into experienced Seedling booth and cultivates
2-3 month, use tidal irrigation, and periodically spray foliage fertilizer；
Seedling is practiced in sylvan life: removes after cultivating 2-3 month in booth in booth takes out of the woods and cultivates two months；
Transplant: be implanted into soil after two months through sylvan life cultivation and naturally grow.
Embodiment three:
A kind of technology using Pseudobulbus Bletillae (Rhizoma Bletillae) tuber to carry out group cultivation kind, including environment disinfected, substrate preparation, in-bottle seeding, goes out
Bottle, sow and cultivate be cultivate.
The described technology using Pseudobulbus Bletillae (Rhizoma Bletillae) tuber to carry out group cultivation kind, comprises the following steps:
Environment disinfected: by group training operating room, culturing room's sterilization, then HD-850 type table above formula horizontal blast cleaning is worked
Platform carries out disinfection in being placed on group training operating room, it is ensured that workbench is in gnotobasis in working area；
Substrate preparation: sandy soil or folder sandy soil, fertile soil and wood sawdust are done by proportion 2:2:1 mix homogeneously and trains in groups
Seedling substrate, standby；
In-bottle seeding: take Pseudobulbus Bletillae (Rhizoma Bletillae) tuber, through sterilization treatment, as the outer implant of detoxication and tissue culture, by be cultivated white
And tissue cultured seedling move to booth together with Seedling bottle in carry out seedling exercising, place 10-15 days under indoor natural light, then bottle cap opened, put
Put 1 day；
Bottle outlet: have the tweezers of Cotton Gossypii take out tissue cultured seedling and the culture medium of its root cleaned by head bundle, makes tissue cultured seedling to the greatest extent
Individual plant may be divided into；Cleaned tissue cultured seedling is put into ventilating and cooling place and places a few hours, then cover tissue cultured seedling with wet towel
Blade, and only root is exposed, finally root is placed in carbendazim aqueous solution immersion several minutes, transfers to root-growing agent water-soluble
Liquid soaks several minutes；
Booth seedling exercising is trained: is taken out from culture bottle by seedling, with 800-1000 times of carbendazol soaking and washing 2-3 time, general
The culture medium of root is washed off, in then compost Seedling dish is arrived in plantation, the Seedling dish having planted Pseudobulbus Bletillae (Rhizoma Bletillae) Seedling is put into experienced Seedling booth and cultivates
2-3 month, use tidal irrigation, and periodically spray foliage fertilizer；
Seedling is practiced in sylvan life: removes after cultivating 2-3 month in booth in booth takes out of the woods and cultivates two months；
Transplant: be implanted into soil after two months through sylvan life cultivation and naturally grow.
In the present embodiment, transplanting tissue cultured seedling 300, finally survival 292, survival rate reaches 97.4%.
The invention has the beneficial effects as follows: owing to the component of tissue cultured seedling substrate is by sandy soil or folder sandy soil, fertile soil and saw
Wood flour forms so that soil permeability is good, the most beneficially the growth of Pseudobulbus Bletillae (Rhizoma Bletillae) tissue cultured seedling；Transplanting method by clean tissue cultured seedling with 3
It it is one clump, by the mode surface planting of row Herba Alii fistulosi to seedbed, it is possible to be effectively increased tissue cultured seedling survival rate；There is provided simultaneously one applicable
Transition method from test bottle to the Nature, make tissue cultured seedling the most enough quickly improve the absorption function of its root hair, cane dredge fortune
Transmission function, the photosynthesis function of blade, can reform of nature environment quickly, improve the transplanting survival rate of tissue cultured seedling.
The above, the only detailed description of the invention of the present invention, but protection scope of the present invention is not limited thereto, and any
The change expected without creative work or replacement, all should contain and be as the criterion at the new protection domain of this practicality.
Claims (2)
1. one kind uses the technology that Pseudobulbus Bletillae (Rhizoma Bletillae) tuber carries out group cultivation kind, it is characterised in that: include environment disinfected, substrate preparation, bottle
Interior seedling exercising, bottle outlet, sow and cultivate be cultivate.
2. the technology using Pseudobulbus Bletillae (Rhizoma Bletillae) tuber to carry out group cultivation kind as claimed in claim 1, it is characterised in that: include following step
Rapid:
1) environment disinfected: by group training operating room, culturing room's sterilization, then by HD-850 type table above formula horizontal blast clean bench
Carry out disinfection in being placed on group training operating room, it is ensured that workbench is in gnotobasis in working area；
2) substrate preparation: sandy soil or folder sandy soil, fertile soil and wood sawdust mix homogeneously are made tissue cultured seedling substrate, standby；
3) in-bottle seeding: take Pseudobulbus Bletillae (Rhizoma Bletillae) tuber, through sterilization treatment, as the outer implant of detoxication and tissue culture, by Pseudobulbus Bletillae (Rhizoma Bletillae) to be cultivated
Tissue cultured seedling carries out seedling exercising in moving to booth together with Seedling bottle, transfers 10-15 days at indoor natural light, then is opened by bottle cap, places 1
My god；
4) bottle outlet: have the tweezers of Cotton Gossypii take out tissue cultured seedling and the culture medium of its root cleaned by head bundle, make tissue cultured seedling to the greatest extent may be used
Individual plant can be divided into；Cleaned tissue cultured seedling is put into ventilating and cooling place and places a few hours, then cover tissue cultured seedling with wet towel
Blade, and only root is exposed, finally root is placed in carbendazim aqueous solution immersion several minutes, transfers to root-growing agent aqueous solution
Middle immersion several minutes；
5) booth seedling exercising training: taken out from culture bottle by seedling, by 800-1000 times of carbendazol soaking and washing 2-3 time, by root
The culture medium in portion is washed off, in then compost Seedling dish is arrived in plantation, the Seedling dish having planted Pseudobulbus Bletillae (Rhizoma Bletillae) Seedling is put into experienced Seedling booth and cultivates 2-3
Individual month, use tidal irrigation, and periodically spray foliage fertilizer；
6) Seedling is practiced in sylvan life: removes after cultivating 2-3 month in booth in booth takes out of the woods and cultivates two months；
7) transplant: be implanted into soil after two months through sylvan life cultivation and naturally grow.
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| CN201610617266.XA CN106069780B (en) | 2016-07-29 | 2016-07-29 | A technique for tissue culture breeding is carried out using bletilla stem tuber |
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| CN201610617266.XA CN106069780B (en) | 2016-07-29 | 2016-07-29 | A technique for tissue culture breeding is carried out using bletilla stem tuber |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107466654A (en) * | 2017-09-12 | 2017-12-15 | 六盘水黔丰农业发展有限公司 | A kind of efficiently method of reproducing bletilla striata tissue-cultured seedling and the growth belt method of the bletilla striata |
| CN114982617A (en) * | 2022-06-27 | 2022-09-02 | 武汉兰多生物科技有限公司 | Water culture seedling hardening method for improving transplanting survival rate of Arundo donax tissue culture seedlings |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103314854A (en) * | 2013-07-08 | 2013-09-25 | 重庆市秀山红星中药材开发有限公司 | Domestication cultivation method of bletilla tissue culture seedlings |
-
2016
- 2016-07-29 CN CN201610617266.XA patent/CN106069780B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103314854A (en) * | 2013-07-08 | 2013-09-25 | 重庆市秀山红星中药材开发有限公司 | Domestication cultivation method of bletilla tissue culture seedlings |
Non-Patent Citations (1)
| Title |
|---|
| 郭乔仪等: ""白芨组培苗春季剪除茎叶炼苗技术"", 《农村实用技术》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107466654A (en) * | 2017-09-12 | 2017-12-15 | 六盘水黔丰农业发展有限公司 | A kind of efficiently method of reproducing bletilla striata tissue-cultured seedling and the growth belt method of the bletilla striata |
| CN114982617A (en) * | 2022-06-27 | 2022-09-02 | 武汉兰多生物科技有限公司 | Water culture seedling hardening method for improving transplanting survival rate of Arundo donax tissue culture seedlings |
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| CN106069780B (en) | 2018-07-20 |
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