CN108029555A - A kind of bletilla striata method for culturing seedlings - Google Patents
A kind of bletilla striata method for culturing seedlings Download PDFInfo
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- CN108029555A CN108029555A CN201711285164.3A CN201711285164A CN108029555A CN 108029555 A CN108029555 A CN 108029555A CN 201711285164 A CN201711285164 A CN 201711285164A CN 108029555 A CN108029555 A CN 108029555A
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- 238000000034 method Methods 0.000 title claims abstract description 27
- 241001313857 Bletilla striata Species 0.000 title claims abstract description 12
- 238000012258 culturing Methods 0.000 title claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000011159 matrix material Substances 0.000 claims abstract description 19
- 239000001963 growth medium Substances 0.000 claims abstract description 17
- 239000003337 fertilizer Substances 0.000 claims abstract description 13
- 201000010099 disease Diseases 0.000 claims abstract description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 7
- 235000015097 nutrients Nutrition 0.000 claims abstract description 6
- 239000007921 spray Substances 0.000 claims description 13
- TWFZGCMQGLPBSX-UHFFFAOYSA-N carbendazim Chemical compound C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 12
- 238000005286 illumination Methods 0.000 claims description 11
- 230000003442 weekly effect Effects 0.000 claims description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims description 7
- 229920001817 Agar Polymers 0.000 claims description 5
- 238000011534 incubation Methods 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 239000008272 agar Substances 0.000 claims description 4
- 239000006013 carbendazim Substances 0.000 claims description 4
- 235000013399 edible fruits Nutrition 0.000 claims description 4
- 230000000855 fungicidal effect Effects 0.000 claims description 4
- 239000000417 fungicide Substances 0.000 claims description 4
- 239000004576 sand Substances 0.000 claims description 4
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims description 3
- 241000196324 Embryophyta Species 0.000 claims description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- 239000005784 Fluoxastrobin Substances 0.000 claims description 3
- 235000018290 Musa x paradisiaca Nutrition 0.000 claims description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- 229930006000 Sucrose Natural products 0.000 claims description 3
- 150000001413 amino acids Chemical class 0.000 claims description 3
- 239000003599 detergent Substances 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- UFEODZBUAFNAEU-NLRVBDNBSA-N fluoxastrobin Chemical compound C=1C=CC=C(OC=2C(=C(OC=3C(=CC=CC=3)Cl)N=CN=2)F)C=1C(=N/OC)\C1=NOCCO1 UFEODZBUAFNAEU-NLRVBDNBSA-N 0.000 claims description 3
- 239000004021 humic acid Substances 0.000 claims description 3
- 229960002523 mercuric chloride Drugs 0.000 claims description 3
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 claims description 3
- 239000003415 peat Substances 0.000 claims description 3
- 238000005507 spraying Methods 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 239000005720 sucrose Substances 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 239000000499 gel Substances 0.000 claims description 2
- 240000005561 Musa balbisiana Species 0.000 claims 1
- 239000002917 insecticide Substances 0.000 claims 1
- 230000035784 germination Effects 0.000 abstract description 5
- 239000002609 medium Substances 0.000 abstract description 3
- 239000012499 inoculation medium Substances 0.000 abstract 1
- 230000003020 moisturizing effect Effects 0.000 abstract 1
- 241001313855 Bletilla Species 0.000 description 5
- 238000009331 sowing Methods 0.000 description 4
- 240000008790 Musa x paradisiaca Species 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000002775 capsule Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000012869 germination medium Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000012090 tissue culture technique Methods 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 244000082204 Phyllostachys viridis Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000009406 nutrient management Methods 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 150000003217 pyrazoles Chemical class 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Abstract
The present invention provides a kind of bletilla striata method for culturing seedlings, described method includes following steps：Whole seedling raising process is divided into two stages.First stage, by seed uniform broadcasting to culture medium top layer, carries out sprouting culture in tissue culture room；Enter second stage culture after seed is sprouted and to form protocorm, protocorm is peeled off from culture medium and into the water, make uniformly to be spread out on seedbed after it is scattered, make protocorm exposed on matrix, periodically seedbed is sprayed with nutrient solution and pay attention to seedbed moisturizing, temperature control and shading, prevent direct sunlight, and carry out water and fertilizer management and disease control management.Germination efficiency of the present invention is high and sprout time is short, management is easy, seedling cost is low.It can complete within 10 15 days to sprout, the culture of tissue culture room is completed after 30 to 35 days；The present invention only needs an inoculation medium, is disseminated to after protocorm to be formed on seedling medium and carries out nursery.
Description
Basic area
The invention belongs to seedling-raising technique field, and in particular to a kind of bletilla striata method for culturing seedlings.
Background technology
The bletilla striata is a kind of traditional Chinese medicine in China, and its seed is because no endosperm causes in nature under germination rate,
Existing wild resource is increasingly reduced, and in order to meet the needs of people are to the medicinal material, people begin attempt to artificial breeding and cultivation.Now
Common bletilla striata method for culturing seedlings is broadly divided into two classes：The fast numerous and direct sowing and seedling of plant tissue culture.
In the prior art, traditional tissue culture technique nursery is needed by once sowing and culture medium of transferring twice, people
Work cost is very high；And direct sowing and seedling germination rate is too low, less than 5%, thus it is especially big to seed demand, and protocorm after sprouting
Growth is very slow, causes the seedling cycle very long, more than 1 year, nursery increased risk.
Although some scholars and production unit have carried out this good try, as CN201410364786,
CN201610065099、CN201410364717、《Wild bletilla striata artificial breeding technique》With《White loft tissue culture technique system is ground
Study carefully》.But, the seedling cost in these researchs is excessive, is unfavorable for the cultivation of the bletilla striata.
The content of the invention
In view of the shortcomings of the prior art, it is an object of the invention to provide a kind of bletilla striata method for culturing seedlings, it is characterised in that institute
The method of stating includes the following steps：
(1) fruit pod after disinfection is cut, seed is shaken off to culture medium top layer, cultivated into the first stage, i.e.,：It is advanced
Row sprouts culture, is sprouted to seed, then carries out Protocorm；
The culture medium is semigel shape culture medium, its formula is：
3.5g/L high intensity agar, sucrose 30g/L, NAA 0.5mg/L, banana 20g/L, 1/2MS, pH is 5.85~5.9；
It is described sprout cultivation stage when condition of culture be：
Temperature is 20~25 DEG C, and intensity of illumination is 1500~2200Lux, when light application time is 10 small/day；
Condition of culture during the Protocorm stage is：
Temperature is 20~25 DEG C, and intensity of illumination is 2500~3000Lux, when light application time is 12 small/day；
(2) protocorm stem length is to 2~3mm after step (1) culture, into second stage culture, i.e.,：Take out protocorm simultaneously
Spread out in water, then protocorm is uniformly spread out in the matrix in seedbed, make protocorm exposed on matrix, located
Reason process tries not to injure young shoot；
(3) protocorm in step (2) is sprayed 1~2 time weekly with nutrient solution, and sprays carbendazol；Keep temperature
In 20~25 DEG C and appropriate illumination；
(4) water and fertilizer management and disease control management are carried out；
Wherein, the matrix presses 1 by Denmark's Pin Shi Tops peat and river sand:1 volume ratio mixes；
The formula of the nutrient solution is：1/5MS+0.1mg/LNAA.
Preferably, in step (1), the method for the disinfection is：In order, first rinsed with flowing water, then 0.1% liquid detergent is molten
Liquid rinses, then is cleaned with distilled water, is then soaked 30 seconds with 75% alcohol, then is soaked 8~12 minutes with 0.1% mercuric chloride solution,
Finally use aseptic water washing 5~8 times, be put into superclean bench and dry.
Preferably, the gel strength of high intensity agar described in step (1) is 1400g/cm2。
The incubation time of the first stage is 8~12 days；And/or the incubation time of the second stage is 30~35
My god.
Preferably, the seedbed is paved into by the matrix by the specification of wide 1m, thickness 0.2m.
Preferably, in step (2), it is 70~80% to keep seedbed humidity, and temperature is 20~25 DEG C, and sprays appropriate desinsection
Agent.
Preferably, in step (3), in step (3), the concentration of the carbendazol is 0.5 ‰.
Preferably, in step (2), when disseminating protocorm, the density of the bulb is 10~150,000 plants/acre.
Preferably, the water and fertilizer management is：Keep water supply in media；Using amino acid, humic acid fertilizer, early period sprays dense
Spend for one thousandth, weekly spray 1 time；After 1 month, concentration increases to 81 percent, is still to spray 1 time weekly；From second
Start the moon monthly to spray 1 secondary and micro-element fertilizers.
Preferably, the disease control management is：1 fungicide is sprayed weekly, is alternately sprayed using Fluoxastrobin and carbendazim
Apply.
Beneficial effects of the present invention：
(1) present invention can save that seed, germination rate are high and sprout time is short, can complete within 10-15 days to sprout, 30 to
The culture of tissue culture room is completed after 35 days；
(2) type of rearing of the invention only needs to use a kind of culture medium, eliminates the propagation training in traditional incubation
Foster and process of rooting culture, it is not necessary to seedling transit working is manually largely engaged in, is compared with tradition tissue culture, seedling cost
Reduce by 70%；
(3) present invention management is easy, and seedling cost is low.
Brief description of the drawings
Design sketch when Fig. 1 is bletilla striata seedling culture.
Embodiment
The present invention is specifically described below by embodiment, it is necessary to which indicated herein is that following embodiments are simply used
It is further detailed in the present invention, it is impossible to be interpreted as limiting the scope of the invention, which is skilled in technique
Personnel still fall within protection scope of the present invention according to some nonessential modifications and adaptations that foregoing invention content is made.
Embodiment 1
1st, seed is sprouted：
1) capsule sterilizes：Capsule, the rinsing of 0.1% liquid detergent solution first are rinsed with flowing water, distilled water is cleaned, the leaching of 75% alcohol
Bubble 30 seconds, then soaked 10 minutes with 0.1% mercuric chloride solution, last aseptic water washing 5~8 times, is put into superclean bench and dries.
2) germination medium is prepared：Using semigel shape culture medium, it is formulated and is：2.0g/L high intensity agar (gel strengths
1400g/cm2), sucrose 30g/L, NAA 0.5g/L, banana 20g/L, 1/2MS.
3) sow：The fruit pod disinfected with tweezers gripping, with blade cut fruit pod, will shake off the table in germination medium
Layer, is careful not to rock culture medium, in case culture medium rupture causes seed to sink.Condition of culture is：20~25 DEG C of temperature,
4) bulb is induced：Temperature control is in 20~25 DEG C, 1500~2200Lux of intensity of illumination, light application time during beginning
10 it is small when/day.After planting 10 days or so, bletilla seed started to sprout, and increases illumination at this time, illumination is brought up to 2500~
3000Lux, at the same extend light application time to 12 it is small when/day；Culture 30~35 days, bulb can be developed to 2mm or so and loosely divide
Cloth is on culture medium.
2nd, prepared by matrix
Matrix selects high-temperature sterilization Denmark Pin Shi Tops peat and river sand 1：1 ratio mixes, and organic matter enriches in turf
And river sand can increase density of matrix, be conducive to seedling and take root.Proportioned matrix is paved into the seedbed of wide 1m, thickness 20cm.
3rd, bulb is live
The bulb of culture medium to be poured into clear water and is gently agitated for, they can be loosed off completely, be not required to artificial external force and separated,
In order to avoid causing mechanical damage, then bulb is uniformly spread out on seedbed, makes bulb exposed on matrix.Prepare 1/2MS+
The nutrient solution of 0.1mg/LNAA sprays seedbed, on every Mondays to twice, keeps seedbed humidity 70~80%, 20~25 DEG C of temperature.Make
Prevent mould from growing with fungicide (carbendazim).
4th, bulb is transplanted
(1) site preparation：The greenhouse that selection is irrigated, ventilation equipment is complete carries out nursery.It is first that the progress of greenhouse soil is smooth, then divide
Into wide 1.0 meters of seedling bed.Seedling bed first spreads one layer of woollen blanket, the seedling medium of 8~10 cm thicks is repaved, before seedling medium use
High-temperature sterilization is had to pass through, kills grass seeds and the microorganism of the inside.After completing matrix, matrix is irrigated.
Winter is not less than 5 degree, summer cooling, not higher than 25 degree.
(2) bulb disperses：The bulb grown up (2~3mm) is poured into clear water together with culture medium, is gently agitated for, makes bletilla
Bulb scatter, and washes away culture medium, is pulled out bletilla bulb with sieve, then with Bravo medicining liquid dipping 3~5 minutes, pulls out
Draining 5-10 minutes.
(3) bulb is transplanted：The bletilla bulb that Bravo soaked uniformly is sprinkling upon on the seedbed of preparation, per acre probably
Spread 100,000 plants or so.One layer 0.2 centimetre of matrix is spread on surface afterwards, covers bletilla bulb, and is watered again, moistens matrix.
(4) humidity, illumination：After transplanting, pay attention to keeping appropriateness, and reduce intensity of illumination, using sunshade net, with bamboo chip branch
Rise, can shading be retentive of moisture again.
(5) water and fertilizer management：After transplanting, matrix moistening degree is observed daily, pays attention to keeping water supply in media.After transplanting 10 days,
Bulb progressively grows seedling, should strengthen nutrient management.Using amino acid, humic acid fertilizer, early period spraying concentration for thousand/
One, spray 1 time weekly.After 1 month, concentration increases to 1/800th, is still to spray 1 time weekly.Monthly sprayed since second month
1 secondary and micro-element fertilizers is applied, is sprayed according to purchased Fertilizer application explanation.
(6) disease control：Disease control is paid attention to during nursery, after transplanting 2 weeks, ventilation is paid attention to, prevents seedling from falling ill.Weekly
Spray 1 fungicide, alternately sprayed using pyrazoles Fluoxastrobin and carbendazim, to prevent developing immunity to drugs, spraying concentration early period according to
Specification halves use, is sprayed to specifications after 2 months.
In the present embodiment, up to more than 96%, sowing can just sprout gained germination rate after 10 days.
After 5 months, bletilla striata seedling can grow to 15-20 centimetres of height, and 2-3 centimetres of bulb, reaches transplanting standard.
Claims (10)
1. a kind of bletilla striata method for culturing seedlings, it is characterised in that described method includes following steps：
(1) fruit pod after disinfection is cut, seed is shaken off to culture medium top layer, cultivated into the first stage, i.e.,：First sprouted
Hair culture, sprouts to seed, then carries out Protocorm；
The culture medium is semigel shape culture medium, its formula is：
3.5g/L high intensity agar, sucrose 30g/L, NAA 0.5mg/L, banana 20g/L, 1/2MS, pH is 5.85~5.9；
It is described sprout cultivation stage when condition of culture be：
Temperature is 20~25 DEG C, and intensity of illumination is 1500~2200Lux, when light application time is 10 small/day；
Condition of culture during the Protocorm stage is：
Temperature is 20~25 DEG C, and intensity of illumination is 2500~3000Lux, when light application time is 12 small/day；
(2) protocorm stem length is to 2~3mm after step (1) culture, into second stage culture, i.e.,：Take out protocorm and in water
It is middle to be spread out, then protocorm is uniformly spread out in the matrix in seedbed, make protocorm exposed on matrix, treat
Journey tries not to injure young shoot；
(3) protocorm in step (2) is sprayed 1~2 time weekly with nutrient solution, and sprays carbendazol；Maintain the temperature at 20
~25 DEG C and appropriate illumination；
(4) water and fertilizer management and disease control management are carried out；
Wherein, the matrix presses 1 by Denmark's Pin Shi Tops peat and river sand:1 volume ratio mixes；
The formula of the nutrient solution is：1/5MS+0.1mg/LNAA.
2. according to the method described in claim 1, it is characterized in that, in step (1), the method for the disinfection is：In order, first
Rinsed with flowing water, then the rinsing of 0.1% liquid detergent solution, then cleaned with distilled water, then soaked 30 seconds with 75% alcohol, then use
0.1% mercuric chloride solution soaks 8~12 minutes, finally with aseptic water washing 5~8 times, is put into superclean bench and dries.
3. according to the method described in claim 1, it is characterized in that, the gel strength of high intensity agar is described in step (1)
1400g/cm2。
4. according to the method described in claim 1, it is characterized in that, the incubation time of the first stage is 8~12 days；With/
Or, the incubation time of the second stage is 30~35 days.
5. according to the method described in claim 1, it is characterized in that, rule of the seedbed by the matrix by width 1m, thickness 0.2m
Lattice are paved into.
6. according to the method described in claim 1, it is characterized in that, in step (2), it is 70~80% to keep seedbed humidity, temperature
Spend for 20~25 DEG C, and spray appropriate fungicide and insecticide.
7. according to the method described in claim 1, it is characterized in that, in step (3), the concentration of the carbendazol is 0.5 ‰.
8. according to the method described in claim 1, it is characterized in that, in step (2), when disseminating protocorm, the ball
The density of stem is 10~150,000 plants/acre.
9. according to the method described in claim 1, it is characterized in that, the water and fertilizer management is：Keep water supply in media；Use amino
Acid, humic acid fertilizer, early period, spraying concentration was 1 ‰, was sprayed 1 time weekly；After 1 month, concentration increases to 1.5 ‰, is still every
Week spray 1 time；1 secondary and micro-element fertilizers is monthly sprayed since second month.
10. according to the method described in claim 1, it is characterized in that, the disease control management is：1 sterilization is sprayed weekly
Agent, is alternately sprayed using Fluoxastrobin and carbendazim.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711285164.3A CN108029555B (en) | 2017-12-07 | 2017-12-07 | A kind of bletilla striata method for culturing seedlings |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711285164.3A CN108029555B (en) | 2017-12-07 | 2017-12-07 | A kind of bletilla striata method for culturing seedlings |
Publications (2)
| Publication Number | Publication Date |
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| CN108029555A true CN108029555A (en) | 2018-05-15 |
| CN108029555B CN108029555B (en) | 2019-11-29 |
Family
ID=62096240
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110100677A (en) * | 2019-04-25 | 2019-08-09 | 浙江省农业科学院 | A kind of method of bletilla kind nursery |
| CN111296261A (en) * | 2020-03-02 | 2020-06-19 | 内蒙古自治区生物技术研究院 | Astragalus membranaceus seedling culture method with high content of astragaloside |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105493861A (en) * | 2015-12-24 | 2016-04-20 | 陕西师范大学 | Method for directly seeding bletilla striata protocorms |
| CN107896851A (en) * | 2017-12-12 | 2018-04-13 | 遵义医学院 | A kind of live fast numerous method of bletilla earth culture |
-
2017
- 2017-12-07 CN CN201711285164.3A patent/CN108029555B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105493861A (en) * | 2015-12-24 | 2016-04-20 | 陕西师范大学 | Method for directly seeding bletilla striata protocorms |
| CN107896851A (en) * | 2017-12-12 | 2018-04-13 | 遵义医学院 | A kind of live fast numerous method of bletilla earth culture |
Non-Patent Citations (1)
| Title |
|---|
| 汤兴利等: "白及种子液体悬浮培养原球茎的研究", 《安徽农业科学》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110100677A (en) * | 2019-04-25 | 2019-08-09 | 浙江省农业科学院 | A kind of method of bletilla kind nursery |
| CN111296261A (en) * | 2020-03-02 | 2020-06-19 | 内蒙古自治区生物技术研究院 | Astragalus membranaceus seedling culture method with high content of astragaloside |
| CN111296261B (en) * | 2020-03-02 | 2021-09-21 | 内蒙古自治区生物技术研究院 | Astragalus membranaceus seedling culture method with high content of astragaloside |
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| Publication number | Publication date |
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| CN108029555B (en) | 2019-11-29 |
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