CN107466654A - A kind of efficiently method of reproducing bletilla striata tissue-cultured seedling and the growth belt method of the bletilla striata - Google Patents

A kind of efficiently method of reproducing bletilla striata tissue-cultured seedling and the growth belt method of the bletilla striata Download PDF

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Publication number
CN107466654A
CN107466654A CN201710817885.8A CN201710817885A CN107466654A CN 107466654 A CN107466654 A CN 107466654A CN 201710817885 A CN201710817885 A CN 201710817885A CN 107466654 A CN107466654 A CN 107466654A
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Prior art keywords
bletilla striata
soil
bletilla
tissue
seedling
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Inventor
周明辉
曹强
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Liupanshui Qianfeng Agricultural Development Co Ltd
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Liupanshui Qianfeng Agricultural Development Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01BSOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
    • A01B79/00Methods for working soil
    • A01B79/02Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008Methods for regeneration to complete plants

Abstract

The present invention proposes a kind of efficiently method of reproducing bletilla striata tissue-cultured seedling and the growth belt method of the bletilla striata, comprises the following steps:Bletilla tissue culture seedlings method, initial point selection, between annual 10 November, and before capsule prematurity cracking, select by the use of the seed in the fresh capsule of the bletilla striata and be used as tissue culture explant, seed disinfection, bletilla striata seeds immersion certain time is carried out disinfection with thimerosal, then pulls out, dry and preserve in an aseptic environment, culturing room sterilizes, culturing room is subjected to sterilizing, ensures that culturing room is in gnotobasis, bletilla striata seeds culture.The method of the efficient reproducing bletilla striata tissue-cultured seedling and the growth belt method of the bletilla striata, the culture of bletilla tissue culture seedlings can be carried out by preferable bletilla striata seeds, and the soil by preferably applying fertilizer is planted to bletilla tissue culture seedlings, the quality and yield of the bletilla striata can be effectively improved, make benefit, and farm manure can rationally be utilized, reduced environmental pollution.

Description

A kind of efficiently method of reproducing bletilla striata tissue-cultured seedling and the growth belt method of the bletilla striata
Technical field
The present invention relates to bletilla striata planting technology field, specially a kind of efficiently method of reproducing bletilla striata tissue-cultured seedling and the bletilla striata Growth belt method.
Background technology
The bletilla striata, also known as Lian Jicao, Gan Gen, give free of charge, indocalamus orchid, Zhu Lan, purple blue, purple a species of orchid, hundred large bamboo hats with a conical crown and broad brim, perennial herb napiform root plants Thing, plant is high 18-60 centimetres, is mainly distributed on China, Japan and Upper Myanmar.The main florescence in spring, but according to each ground vapour The difference of time, late Winter Solstice early summer may all bloom, and the bletilla striata has extensive medical value and Ornamental value, is mainly used in convergence and stops Blood, detumescence and promoting granulation, flower have the colors such as purplish red, white, blue, yellow and powder, can potted plant indoor appreciation, can also intersperse in the flower more covered One jiao of platform, flower border or garden, existing bletilla striata implantation methods typically use bletilla striata tissue and carry out bletilla striata seedling training by nutrient solution Support, then bletilla striata seedling is planted, but use the bletilla striata tissue carry out bletilla striata seedling incubation in, the bletilla striata is not organized into Row is preferred, easily lowers the overall yield of the bletilla striata, and excessively rough in terms of soil selection, easily influences bletilla striata growth.
The content of the invention
The technical problem to be solved in the present invention is to overcome the defects of existing, there is provided a kind of side of efficiently reproducing bletilla striata tissue-cultured seedling Method and the growth belt method of the bletilla striata, the culture of bletilla tissue culture seedlings can be carried out by preferable bletilla striata seeds, and by preferably applying The soil of fertilizer is planted to bletilla tissue culture seedlings, can be effectively improved the quality and yield of the bletilla striata, be made benefit, and can Rationally to be utilized to farm manure, reduce environmental pollution, effectively can solve the problems, such as in background technology.
To achieve the above object, the present invention proposes:A kind of efficiently growth belt of the method for reproducing bletilla striata tissue-cultured seedling and the bletilla striata Method, comprise the following steps:
Bletilla tissue culture seedlings method;
1)Initial point selection:Between annual 10-11 months, and before capsule prematurity cracking, select with the fresh capsule of the bletilla striata Seed as tissue culture explant;
2)Seed disinfection:Bletilla striata seeds immersion certain time is carried out disinfection with thimerosal, then pulls out, dry and in asepsis ring Preserved under border;
3)Culturing room sterilizes:Culturing room is subjected to sterilizing, ensures that culturing room is in gnotobasis;
4)Bletilla striata seeds culture:The bletilla striata seeds sterilized after drying are placed in seed culture medium and cultivated, then will culture Base is placed in culturing room, until seed grows up to seedling;
5)Bletilla striata seedling is cultivated:Transplant seedlings in seedling culture medium and cultivate 3-4 months, obtain bletilla tissue culture seedlings;
Bletilla striata implantation methods:
1)Select soil:Loose fertile sandy loam and humus loam are selected, makes the environment partial temperature in soil warm and slightly cloudy It is wet;
2)Ploughing soil:Using turning over car by ploughing soil, and ploughing soil depth is at 20-30 centimetres;
3)Soil fertilizing:Every mu of ground applies 500 kilograms of farm manure, and applies 30 kilograms of chemical fertilizer;
4)It is secondary to turn over:Reuse and turn over car and turned over the soil after fertilising;And soil block revolved by rotary cultivator it is small, and Soil is raked whole;
5)Ditch in soil:The soil raked is ditched using agricultural ditching machine, and makes ditch equidistantly distributed;
6)Transplanting plantation:The bletilla striata is trained to the bletilla striata seedling turned out in seedling-growing method and carries out transplanting plantation;And make adjacent two bletilla striata seedlings Between spacing be 9-12 centimetres;
7)Final-period management:Tillage and weeding, real-time top dressing, irrigation and drainage, 7-9 months pour the bletilla striata water twice daily, and have heavy rain When, draining is carried out in time.
As a preferred technical solution of the present invention, in step 2, thimerosal uses 0.1% or so HgCl, during sterilization Between be 20-25 minutes, then using aseptic water washing 3-4 times, each 2-3 minutes.
As a preferred technical solution of the present invention, in step 7, during tillage and weeding, 5-6 months plant ground to bletilla striata seed Band carries out weeding, and the depth of weeding uses 70% thiophanate methyl WP, 1000 times of dilution dialogues at 5-15 centimetres Splendid achnatherum seedling is sprayed.
As a preferred technical solution of the present invention, in step 7, in the real-time top dressing of final-period management, every month applies Fertilizer, and farm manure or phosphate fertilizer, the mixture of nitrogenous fertilizer and potash fertilizer can be selected in fertilizer.
As a preferred technical solution of the present invention, in the step 5 of bletilla striata implantation methods, when soil is ditched, adjacent two Spacing between bar ditch is 20-25 centimetres, and the depth of ditch is 5-8 centimetres.
Compared with prior art, the beneficial effects of the invention are as follows:Bletilla striata tissue culture can be carried out by preferable bletilla striata seeds The culture of seedling, and the soil by preferably applying fertilizer is planted to bletilla tissue culture seedlings, can effectively improve quality and the production of the bletilla striata Amount, makes benefit, and farm manure rationally can be utilized, and reduces environmental pollution.
Brief description of the drawings
Fig. 1 is a kind of efficiently flow chart of the method for reproducing bletilla striata tissue-cultured seedling and the growth belt method of the bletilla striata of the present invention.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.It is based on Embodiment in the present invention, those of ordinary skill in the art are obtained every other under the premise of creative work is not made Embodiment, belong to the scope of protection of the invention.
Referring to Fig. 1, the present invention provides following technical scheme:
Embodiment one:A kind of efficiently method of reproducing bletilla striata tissue-cultured seedling and the growth belt method of the bletilla striata, comprise the following steps:
Bletilla tissue culture seedlings method;
1)Initial point selection:Between annual 10-11 months, and before capsule prematurity cracking, select with the fresh capsule of the bletilla striata Seed as tissue culture explant;
2)Seed disinfection:Bletilla striata seeds immersion certain time is carried out disinfection with thimerosal, then pulls out, dry and in asepsis ring Preserved under border, thimerosal uses 0.1% or so HgCl, and disinfecting time is 20 minutes, then using aseptic water washing 3 times, every time 2 minutes;
3)Culturing room sterilizes:Culturing room is subjected to sterilizing, ensures that culturing room is in gnotobasis;
4)Bletilla striata seeds culture:The bletilla striata seeds sterilized after drying are placed in seed culture medium and cultivated, then will culture Base is placed in culturing room, until seed grows up to seedling;
5)Bletilla striata seedling is cultivated:Transplant seedlings in seedling culture medium and cultivate 3-4 months, obtain bletilla tissue culture seedlings;
Bletilla striata implantation methods:
1)Select soil:Loose fertile sandy loam and humus loam are selected, makes the environment partial temperature in soil warm and slightly cloudy It is wet;
2)Ploughing soil:Using turning over car by ploughing soil, and ploughing soil depth is at 20 centimetres;
3)Soil fertilizing:Every mu of ground applies 500 kilograms of farm manure, and applies 30 kilograms of chemical fertilizer;
4)It is secondary to turn over:Reuse and turn over car and turned over the soil after fertilising;And soil block revolved by rotary cultivator it is small, and Soil is raked whole;
5)Ditch in soil:The soil raked is ditched using agricultural ditching machine, and makes ditch equidistantly distributed, soil trench digging When, the spacing between adjacent two bar ditch is 20 centimetres, and the depth of ditch is 5 centimetres;
6)Transplanting plantation:The bletilla striata is trained to the bletilla striata seedling turned out in seedling-growing method and carries out transplanting plantation;And make adjacent two bletilla striata seedlings Between spacing be 9-12 centimetres;
7)Final-period management:Tillage and weeding, during tillage and weeding, 5-6 months area is planted to bletilla striata seed and carry out weeding, the depth of weeding Bletilla striata seedling is sprayed at 5 centimetres, and using 70% thiophanate methyl WP, 1000 times of dilutions, real-time top dressing, During the real-time top dressing of final-period management, apply a fertilizer every month, and farm manure or phosphate fertilizer, nitrogenous fertilizer and potash fertilizer can be selected in fertilizer Mixture, irrigation and drainage, 7-9 months pour the bletilla striata water twice daily, and when having heavy rain, to carry out draining in time.
Embodiment two:A kind of efficiently method of reproducing bletilla striata tissue-cultured seedling and the growth belt method of the bletilla striata, comprise the following steps:
Bletilla tissue culture seedlings method;
1)Initial point selection:Between annual 10-11 months, and before capsule prematurity cracking, select with the fresh capsule of the bletilla striata Seed as tissue culture explant;
2)Seed disinfection:Bletilla striata seeds immersion certain time is carried out disinfection with thimerosal, then pulls out, dry and in asepsis ring Preserved under border, thimerosal uses 0.1% or so HgCl, and disinfecting time is 22 minutes, then using aseptic water washing 3 times, every time 2 minutes;
3)Culturing room sterilizes:Culturing room is subjected to sterilizing, ensures that culturing room is in gnotobasis;
4)Bletilla striata seeds culture:The bletilla striata seeds sterilized after drying are placed in seed culture medium and cultivated, then will culture Base is placed in culturing room, until seed grows up to seedling;
5)Bletilla striata seedling is cultivated:Transplant seedlings in seedling culture medium and cultivate 3-4 months, obtain bletilla tissue culture seedlings;
Bletilla striata implantation methods:
1)Select soil:Loose fertile sandy loam and humus loam are selected, makes the environment partial temperature in soil warm and slightly cloudy It is wet;
2)Ploughing soil:Using turning over car by ploughing soil, and ploughing soil depth is at 23 centimetres;
3)Soil fertilizing:Every mu of ground applies 500 kilograms of farm manure, and applies 30 kilograms of chemical fertilizer;
4)It is secondary to turn over:Reuse and turn over car and turned over the soil after fertilising;And soil block revolved by rotary cultivator it is small, and Soil is raked whole;
5)Ditch in soil:The soil raked is ditched using agricultural ditching machine, and makes ditch equidistantly distributed, soil trench digging When, the spacing between adjacent two bar ditch is 21 centimetres, and the depth of ditch is 6 centimetres;
6)Transplanting plantation:The bletilla striata is trained to the bletilla striata seedling turned out in seedling-growing method and carries out transplanting plantation;And make adjacent two bletilla striata seedlings Between spacing be 10 centimetres;
7)Final-period management:Tillage and weeding, during tillage and weeding, 5-6 months area is planted to bletilla striata seed and carry out weeding, the depth of weeding Bletilla striata seedling is sprayed at 5 centimetres, and using 70% thiophanate methyl WP, 1000 times of dilutions, real-time top dressing, During the real-time top dressing of final-period management, apply a fertilizer every month, and farm manure or phosphate fertilizer, nitrogenous fertilizer and potash fertilizer can be selected in fertilizer Mixture, irrigation and drainage, 7-9 months pour the bletilla striata water twice daily, and when having heavy rain, to carry out draining in time.
Embodiment three:A kind of efficiently method of reproducing bletilla striata tissue-cultured seedling and the growth belt method of the bletilla striata, comprise the following steps:
Bletilla tissue culture seedlings method;
1)Initial point selection:Between annual 10-11 months, and before capsule prematurity cracking, select with the fresh capsule of the bletilla striata Seed as tissue culture explant;
2)Seed disinfection:Bletilla striata seeds immersion certain time is carried out disinfection with thimerosal, then pulls out, dry and in asepsis ring Preserved under border, thimerosal uses 0.1% or so HgCl, and disinfecting time is 24 minutes, then using aseptic water washing 4 times, every time 3 minutes;
3)Culturing room sterilizes:Culturing room is subjected to sterilizing, ensures that culturing room is in gnotobasis;
4)Bletilla striata seeds culture:The bletilla striata seeds sterilized after drying are placed in seed culture medium and cultivated, then will culture Base is placed in culturing room, until seed grows up to seedling;
5)Bletilla striata seedling is cultivated:Transplant seedlings in seedling culture medium and cultivate 3-4 months, obtain bletilla tissue culture seedlings;
Bletilla striata implantation methods:
1)Select soil:Loose fertile sandy loam and humus loam are selected, makes the environment partial temperature in soil warm and slightly cloudy It is wet;
2)Ploughing soil:Using turning over car by ploughing soil, and ploughing soil depth is at 27 centimetres;
3)Soil fertilizing:Every mu of ground applies 500 kilograms of farm manure, and applies 30 kilograms of chemical fertilizer;
4)It is secondary to turn over:Reuse and turn over car and turned over the soil after fertilising;And soil block revolved by rotary cultivator it is small, and Soil is raked whole;
5)Ditch in soil:The soil raked is ditched using agricultural ditching machine, and makes ditch equidistantly distributed, soil trench digging When, the spacing between adjacent two bar ditch is 24 centimetres, and the depth of ditch is 7 centimetres;
6)Transplanting plantation:The bletilla striata is trained to the bletilla striata seedling turned out in seedling-growing method and carries out transplanting plantation;And make adjacent two bletilla striata seedlings Between spacing be 11 centimetres;
7)Final-period management:Tillage and weeding, during tillage and weeding, 5-6 months area is planted to bletilla striata seed and carry out weeding, the depth of weeding Bletilla striata seedling is sprayed at 5 centimetres, and using 70% thiophanate methyl WP, 1000 times of dilutions, real-time top dressing, During the real-time top dressing of final-period management, apply a fertilizer every month, and farm manure or phosphate fertilizer, nitrogenous fertilizer and potash fertilizer can be selected in fertilizer Mixture, irrigation and drainage, 7-9 months pour the bletilla striata water twice daily, and when having heavy rain, to carry out draining in time.
Example IV:A kind of efficiently method of reproducing bletilla striata tissue-cultured seedling and the growth belt method of the bletilla striata, comprise the following steps:
Bletilla tissue culture seedlings method;
1)Initial point selection:Between annual 10-11 months, and before capsule prematurity cracking, select with the fresh capsule of the bletilla striata Seed as tissue culture explant;
2)Seed disinfection:Bletilla striata seeds immersion certain time is carried out disinfection with thimerosal, then pulls out, dry and in asepsis ring Preserved under border, thimerosal uses 0.1% or so HgCl, and disinfecting time is 25 minutes, then using aseptic water washing 4 times, every time 3 minutes;
3)Culturing room sterilizes:Culturing room is subjected to sterilizing, ensures that culturing room is in gnotobasis;
4)Bletilla striata seeds culture:The bletilla striata seeds sterilized after drying are placed in seed culture medium and cultivated, then will culture Base is placed in culturing room, until seed grows up to seedling;
5)Bletilla striata seedling is cultivated:Transplant seedlings in seedling culture medium and cultivate 3-4 months, obtain bletilla tissue culture seedlings;
Bletilla striata implantation methods:
1)Select soil:Loose fertile sandy loam and humus loam are selected, makes the environment partial temperature in soil warm and slightly cloudy It is wet;
2)Ploughing soil:Using turning over car by ploughing soil, and ploughing soil depth is at 30 centimetres;
3)Soil fertilizing:Every mu of ground applies 500 kilograms of farm manure, and applies 30 kilograms of chemical fertilizer;
4)It is secondary to turn over:Reuse and turn over car and turned over the soil after fertilising;And soil block revolved by rotary cultivator it is small, and Soil is raked whole;
5)Ditch in soil:The soil raked is ditched using agricultural ditching machine, and makes ditch equidistantly distributed, soil trench digging When, the spacing between adjacent two bar ditch is 25 centimetres, and the depth of ditch is 8 centimetres;
6)Transplanting plantation:The bletilla striata is trained to the bletilla striata seedling turned out in seedling-growing method and carries out transplanting plantation;And make adjacent two bletilla striata seedlings Between spacing be 12 centimetres;
7)Final-period management:Tillage and weeding, during tillage and weeding, 5-6 months area is planted to bletilla striata seed and carry out weeding, the depth of weeding Bletilla striata seedling is sprayed at 5 centimetres, and using 70% thiophanate methyl WP, 1000 times of dilutions, real-time top dressing, During the real-time top dressing of final-period management, apply a fertilizer every month, and farm manure or phosphate fertilizer, nitrogenous fertilizer and potash fertilizer can be selected in fertilizer Mixture, irrigation and drainage, 7-9 months pour the bletilla striata water twice daily, and when having heavy rain, to carry out draining in time.
Benefit of the present invention:The culture of bletilla tissue culture seedlings can be carried out by preferable bletilla striata seeds, and by preferably applying fertilizer Soil bletilla tissue culture seedlings are planted, the quality and yield of the bletilla striata can be effectively improved, make benefit, and can be with Farm manure is rationally utilized, reduced environmental pollution.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with A variety of changes, modification can be carried out to these embodiments, replace without departing from the principles and spirit of the present invention by understanding And modification, the scope of the present invention is defined by the appended.

Claims (5)

1. a kind of efficiently method of reproducing bletilla striata tissue-cultured seedling and the growth belt method of the bletilla striata, it is characterised in that:Comprise the following steps:
Bletilla tissue culture seedlings method;
1)Initial point selection:Between annual 10-11 months, and before capsule prematurity cracking, select with the fresh capsule of the bletilla striata Seed as tissue culture explant;
2)Seed disinfection:Bletilla striata seeds immersion certain time is carried out disinfection with thimerosal, then pulls out, dry and in asepsis ring Preserved under border;
3)Culturing room sterilizes:Culturing room is subjected to sterilizing, ensures that culturing room is in gnotobasis;
4)Bletilla striata seeds culture:The bletilla striata seeds sterilized after drying are placed in seed culture medium and cultivated, then will culture Base is placed in culturing room, until seed grows up to seedling;
5)Bletilla striata seedling is cultivated:Transplant seedlings in seedling culture medium and cultivate 3-4 months, obtain bletilla tissue culture seedlings;
Bletilla striata implantation methods:
1)Select soil:Loose fertile sandy loam and humus loam are selected, makes the environment partial temperature in soil warm and slightly cloudy It is wet;
2)Ploughing soil:Using turning over car by ploughing soil, and ploughing soil depth is at 20-30 centimetres;
3)Soil fertilizing:Every mu of ground applies 500 kilograms of farm manure, and applies 30 kilograms of chemical fertilizer;
4)It is secondary to turn over:Reuse and turn over car and turned over the soil after fertilising;And soil block revolved by rotary cultivator it is small, and Soil is raked whole;
5)Ditch in soil:The soil raked is ditched using agricultural ditching machine, and makes ditch equidistantly distributed;
6)Transplanting plantation:The bletilla striata is trained to the bletilla striata seedling turned out in seedling-growing method and carries out transplanting plantation;And make adjacent two bletilla striata seedlings Between spacing be 9-12 centimetres;
7)Final-period management:Tillage and weeding, real-time top dressing, irrigation and drainage, 7-9 months pour the bletilla striata water twice daily, and have heavy rain When, draining is carried out in time.
2. a kind of efficiently method of reproducing bletilla striata tissue-cultured seedling according to claim 1 and the growth belt method of the bletilla striata, it is special Sign is:In step 2, thimerosal uses 0.1% or so HgCl, and disinfecting time is 20-25 minutes, is then rushed using sterilized water Wash 3-4 times, each 2-3 minutes.
3. a kind of efficiently method of reproducing bletilla striata tissue-cultured seedling according to claim 1 and the growth belt method of the bletilla striata, it is special Sign is:In step 7, during tillage and weeding, 5-6 months area is planted to bletilla striata seed and carry out weeding, the depth of weeding is at 5-15 lis Rice, and bletilla striata seedling is sprayed using 70% thiophanate methyl WP, 1000 times of dilutions.
4. a kind of efficiently method of reproducing bletilla striata tissue-cultured seedling according to claim 1 and the growth belt method of the bletilla striata, it is special Sign is:In step 7, in the real-time top dressing of final-period management, every month apply a fertilizer, and fertilizer can be selected farm manure or The mixture of phosphate fertilizer, nitrogenous fertilizer and potash fertilizer.
5. a kind of efficiently method of reproducing bletilla striata tissue-cultured seedling according to claim 1 and the growth belt method of the bletilla striata, it is special Sign is:In the step 5 of bletilla striata implantation methods, when soil is ditched, the spacing between adjacent two bar ditch is 20-25 centimetres, and ditch Depth be 5-8 centimetres.
CN201710817885.8A 2017-09-12 2017-09-12 A kind of efficiently method of reproducing bletilla striata tissue-cultured seedling and the growth belt method of the bletilla striata Pending CN107466654A (en)

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CN106258955A (en) * 2016-08-08 2017-01-04 贵州大学 A kind of method inducing Fructus Momordicae albuminous cell seedling
CN106417011A (en) * 2016-08-30 2017-02-22 云南金碧制药有限公司 Wild bletilla striata tissue culture rapid propagation method
CN106718906A (en) * 2016-12-16 2017-05-31 河南红枫种苗股份有限公司 The culture medium and method of a kind of silver-colored side winter creeper tissue cultures
CN106613256A (en) * 2016-12-27 2017-05-10 昆明洪茂种植有限公司 Bletilla striata planting method
CN106717997A (en) * 2017-01-24 2017-05-31 贵州省武陵山药用植物白芨开发有限公司 Promote the bletilla striata stem tuber cultural method for expanding and the bletilla striata stem tuber cultivated by it

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108184589A (en) * 2018-01-22 2018-06-22 云南岐瑞生物科技有限公司 A kind of field production method of pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling
CN108184589B (en) * 2018-01-22 2020-09-15 剑川禹丰种植发展有限公司 Large-field cultivation method for bletilla striata and tissue culture seedlings

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