CN108184589A - A kind of field production method of pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling - Google Patents
A kind of field production method of pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling Download PDFInfo
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- CN108184589A CN108184589A CN201810060343.5A CN201810060343A CN108184589A CN 108184589 A CN108184589 A CN 108184589A CN 201810060343 A CN201810060343 A CN 201810060343A CN 108184589 A CN108184589 A CN 108184589A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
- A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
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Abstract
The invention discloses a kind of field production methods of pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling, include the following steps：1), selection is suitble to the plot of plantation bletilla；2) selected plot is ploughed deeply and hack；3) shading and erection spray irrigation system are carried out to entirely planting plot；4) hardening；5) it transplants；6) field management.The planting technology of the pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling of the present invention only needs can harvest within 2 years, and per mu yield can be up to two and half left and right.The present invention selects suitable plot according to bletilla suitable environment condition, the good growth behind crop field is transplanted for the bletilla striata, environmental condition is provided, further, by site preparation, improve soil hardening, carrying out moise-heat sterilization auxiliary covering with ground sheeting, worm's ovum and grass-seed etc. are killed, realizes that transplanting initial stage without weeds, effectively prevents the pressure seedling phenomenon that Weeds at seedling excessively occurs, solve because the slow-growing plant of bletilla tissue-cultured seedling is short and small, and caused by uprooting weed to the adverse effect of bletilla seedling root.
Description
Technical field
The invention belongs to bletilla technical field of cultivation, and in particular to a kind of field production of pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling
Method.
Background technology
Pale reddish brown trident Herba Phaii tankervilliaes are one of orchid bletilla Bletilla striata (Thunb.) Reichb.f.
Kind, it is other to also have chrysanthemum, white flower, light red flower etc. not of the same race, also distinguished with the small bletilla of pale reddish brown trident in Yunnan.Bletilla is sent out at present
Existing has 6 kinds, and China has 4 kinds, and application that can be different degrees of is in medicine.But medicinal material of the highest excellence belongs to pale reddish brown three
Bletilla (containing pale reddish brown trident Herba Phaii tankervilliaes and the small bletilla of pale reddish brown trident) is pitched, is loaded into the Bletilla striata medicinal materials kind of version Chinese Pharmacopoeia in 2015 just
Two conditions are described in detail, when purple flower, second is that medicinal material stem tuber will have 2~3 claw-like branches, long 1.5~5cm, thickness 0.5
~1.5cm.Either pattern is not purple or flower for purple and does not have claw-like branch most bletilla kinds.Therefore into pedestrian
When work is cultivated, the requirement for selecting pale reddish brown trident bletilla that could meet pharmacopeia is had to.
Bletilla striata medicinal materials are always by excavation wild resource, and with the exhaustion of resource, it is wild to be included in focused protection by country
Plant.People begin attempt to artificial growth bletilla, it is existing have planted using stem tuber and using Plant Tissue Breeding
Technology is planted to breed bletilla seedling, but the field production method of existing bletilla tissue-cultured seedling is also there are many deficiencies,
Wherein training tissue culture seedling cultivation technique is also immature, and hardening is the premise that bletilla tissue-cultured seedling puts into field production, different refinings
Seedling-growing method has the survival rate of hardening, the robustness of seedling a large effect, existing hardening off method not only transplanting survival rate
The low and period is long, oneself becomes the bottleneck of bletilla industrialization.During conventional hardening, often using the side such as strong light hardening, Small plastic shed hardening
Formula, for acclimatization and transplants survival rate about 60% or so, the seedling-slowing stage time is long, high by root rot, stem rot infection probability.Tissue-cultured seedling
There is this section of transplanting domestication of bacterium autotrophy outside sterile heterotrophism to bottle, be very easy to a large amount of dead seedlings；Or bring out the seedling quality come not
The problems such as height, field planting effect difference etc..
Therefore, to solve the above problems, it is necessary to invent a kind of field production method of pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling.
Invention content
The purpose of the present invention is to provide a kind of field production methods of pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling.
The object of the present invention is achieved like this, includes the following steps：
1) selection of land：Select warm moist, soil layer it is deep, it is fertile it is loose, draining is good, the red soil rich in organic matter, height above sea level
1600~2500m；
2) site preparation：30~50cm first is ploughed deeply into the plot chosen, then hack, then exposure 5~8 days, then apply decomposed farmers'
Fertilizer, not less than 3%, finally covers solar heat protection mulch to soil organic matter content to soil, carries out moise-heat sterilization, and retain mulch and cover
Lid removes mulch to before being colonized 2~5 days, you can；
3) 30~50% shading nets that height is 2~3m are built on plantation plot, and from 1.6~1.8m of earth's surface under shading net
Height erection can cover the spray irrigation system in entire plantation plot；
4) hardening：Select start hardening to March September to October or 2 months, including hardening outside in-bottle seeding, cleaning and dipping, bottle；5)
Transplanting：It first applies fertilizer to the subsoil, then builds out high 15~25cm, wide 100~120cm, moisture surface of the ditch heart away from 140~160cm, then will pass through
The pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling of hardening is according in the seeding row spacing field planting to the moisture surface of 20cm × 20cm；
6) field management：Periodically plot is irrigated using spray irrigation system, keeps soil moisture content 40%~55%；After transplanting
First Year every 2~3 months, is applied 100~200kg/ mus of well-rotted farmyard manure, after transplanting 1 year, every 3~4 months, is applied decomposed
100~200kg/ mus of farm manure simultaneously pours and applies 80~100kg of biogas slurry.
Preferably, the hack described in step (2) be by soil be broken to 5~2mm20% of soil particle, 2~
0.2mm50%, 0.2~0.02mm30%.
Preferably, the in-bottle seeding described in step (4) is the sterilization processing that first carries out disinfection to culturing room, then will be pale reddish brown
The culture bottle of trident Herba Phaii tankervilliaes tissue culture rooted seedling gradually takes off lid in culturing room, takes off lid within 2~3 days and finishes, during which environmental condition with
It is consistent during tissue culture；Culture bottle is then transferred to the position of hardening outside bottle, keeps ventilation, controls more than 85% shading, together
When keep temperature be 20~22 DEG C, air humidity be more than 85%, transition culture 5~7 days；Described disinfection processing be
In culturing room 60~120min is sterilized using 1000~1200 μ W/cm2 ultraviolet lights.
Preferably, the cleaning and dipping described in step (4) be by pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling from culture bottle
It takes out, is divided into single plant and is placed in the culture dish of the room temperature clear water equipped with 0.5~2cm depths, impregnate 1~3h, it is then repeated as above to change
Water impregnates 3~6 times, after changing water for the last time, persistently impregnates 1~2 day growth-promoting root, then impregnates seedling using plant extraction liquid
10~20min.
Preferably, then the preparation method of the plant extraction liquid first adds for aloe, garlic, mulberries, giant knotweed is taken to smash to pieces
Enter hot ethanol reflux 15~30min extractions of 6~8 times of weight, the boiling boiling of 8~12 times of weight is then added in into extraction vessel
Complete to ethyl alcohol volatilization, filtering obtains the dregs of a decoction and filtrate, filtrate is placed to room temperature, you can.
Preferably, the outer hardening of the bottle described in step (4) includes the following steps：
A, first time thermophilic hardening：Bletilla tissue culture rooted seedling Jing Guo cleaning and dipping is colonized in the matrix of the first hardening bed,
The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of coconut palm chaff again, and cladding thickness is 1~3cm, is kept
Ventilation controls more than 80% shading, while it is 20~22 DEG C to keep temperature, and air humidity is 80~85%, and water content of substrate is protected
More than 70% is held, hardening 15~30 days；
B, low temperature hardening：Ventilation is kept, controls more than 75% shading, while it is 17~19 DEG C to keep temperature, air humidity is
80%~82%, water content of substrate keeps more than 65%, carries out first low temperature hardening 10~15 days；Ventilation is kept, controls shading
More than 75%, while it is 14~16 DEG C to keep temperature, air humidity is 80%~82%, and water content of substrate keeps more than 65%,
Carry out low temperature hardening 10~15 days again；
C, second of thermophilic hardening：Bletilla tissue culture Jing Guo low temperature hardening is taken root into transplantation of seedlings in the matrix of the second hardening bed,
The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of crop field original soil again, and cladding thickness is 2~4cm,
Ventilation is kept, controls more than 70% shading, while it is 20~22 DEG C to keep temperature, air humidity is 70~80%, and matrix is aqueous
Amount is gradually down to more than 60%, hardening 15~30 days, you can；
D, room temperature hardening：Ventilation is kept, controls more than 70% shading, while temperature is controlled to be consistent with crop field temperature, air
More than 70% humidity, more than 60% water content of substrate, hardening 20~30 days, you can into field planting.
Preferably, the matrix is to make red soil or containing the mixed-matrix for making red soil；The red soil of making refers to
It is less than 1% red soil without clay particle or clay particle content.
Preferably, the matrix of the first hardening bed includes the ingredient of following parts by weight：15~20 parts of coconut palm chaff makes red soil
8~12 parts of soil, 6~10 parts of sawdust, 6~10 parts of vermiculite, 6~10 parts of fertile soil.
Preferably, the matrix of the second hardening bed includes the ingredient of following parts by weight：15~20 parts of field original soil, coconut palm
6~10 parts of chaff, 3~5 parts of the dregs of a decoction；The dregs of a decoction are the extraction dregs of a decoction of aloe, garlic, mulberries and giant knotweed.
Preferably, applying fertilizer to the subsoil as 100~150kg calcium magnesium phosphate to be uniformly spread to plantation plot described in step (5)
Surface.
Compared with prior art, beneficial effects of the present invention：
1st, the cultivation technique of pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling of the invention only needs can harvest within 2 years, and per mu yield can reach
Two and half left and right, effectively shorten cultivation period.
2nd, the present invention selects suitable plot according to bletilla suitable environment condition, and the good life behind crop field is transplanted for bletilla
It is long that environmental condition is provided, it further, by site preparation, is first ploughed deeply, improves soil hardening, pass through the further vegetable soil of exposure
Earth is carrying out moise-heat sterilization auxiliary covering with ground sheeting, is killing worm's ovum and grass-seed etc., realizing that transplanting initial stage without weeds, effectively prevents
The pressure seedling phenomenon that Weeds at seedling excessively occurs, solves because bletilla tissue-cultured seedling is slow-growing, and plant is short and small, and caused by uprooting weed pair
The adverse effect of bletilla seedling root.
3rd, hardening stage by stage of the invention, has the more scientific laundering period, greatly improves the survival rate of transplanting, shortens
Transplanting seedling time；The hardening survival rate of the present invention can reach more than 99%, and field-transplanting survival rate can reach more than 98%.
4th, acclimatization and transplants of the invention greatly reduce miscellaneous bacteria and tissue-cultured seedling are infected, and improve the survival rate of tissue-cultured seedling,
Be conducive to cultivate health seedling, it is significant to improving field planting survival rate and subsequent growth, so as to greatly improve production
Quality and yield lay the first stone.
5th, the present invention passes through the temperature change of thermophilic-low temperature-thermophilic-room temperature during hardening, first passes through preference temperature
Hardening so that just contact external environment seedling can be adapted to smoothly under preference temperature, then using low temperature hardening, be improved kind
Seedling then proceedes to carry out hardening using preference temperature, will grow bad seedling at low temperature to the resistivity of adverse environment
Again strong sprout is developed into, it is consistent with transplanting ground temperature so as to fulfill hardening temperature finally using crop field temperature hardening, it greatly shortens
Raising survival rate.
6th, hardening selection of time science of the invention, no sprouting sprouts, therefore effectively reduces the feelings for hindering bud when washing seedling
Condition.Present invention selection carries out hardening in the rest period of bletilla, improves transplanting survival rate.The present invention is by the prevention and control of plant diseases, pest control and chases after
Fertilizer promotes bletilla strong sprout, further improves the survival rate of field-transplanting.
7th, the present invention is similar to environmental condition during culture at hardening initial stage by rationally carrying out hardening, avoids seedling first
Adverse conditions is grown into the environment to differ greatly, then carries out low temperature hardening, finally in the hardening later stage, with field-transplanting
Environmental condition it is similar, so as to achieve the purpose that gradually to adapt to.Present invention selection autumn and spring carry out hardening, avoid high temperature
The situation that seedling caused by high humidity weather is wilted；By the water supply in high humidity guarantee early period seedling, the later stage is gradually close to naturally wet
Degree so that seedling can adapt to and well-grown, improves survival rate.
8th, the present invention is by first to indoor carry out ultraviolet disinfection, eliminating miscellaneous bacteria that may be present in culturing room, avoiding group
Training seedling start contact external environment resistance it is not strong and may caused by disease, for continue hardening lay the first stone.Again by bottle
Transition culture certain time in bottle in the environment of outer culture, by optimum humiture, effectively prevent bletilla tissue-cultured seedling pair
Environment is not suitable for, so as to which it be helped smoothly to adapt to new environmental condition.The present invention uses the physical disinfection of ultraviolet disinfection
Method safely and effectively, avoids the environmental problem that chemical disinfection method is brought.
9th, on the one hand the present invention changes water by tissue culture rooted seedling is impregnated directly in clear water by repeatedly impregnating, effectively clear
The culture medium in tissue-cultured seedling has been managed, has avoided a large amount of clear water of culture medium consuming for removing root in a short time, and easily cause
The problem of damage of cauline leaf and root, prior one side are can to effectively facilitate kind of a seedling rooting by repeatedly impregnating, and
A great number of elements, trace element in early period culture medium can be used as nutrient solution in clear water, provide nutrition progress for seedling is of short duration
Transition.
10th, the present invention is in first time thermophilic hardening (transplanting initial stage), using the coconut palm chaff of more water-permeable and air permeable, make red soil,
Sawdust etc., anti-ponding, loose ventilative, fertility is high, prevents rotten, the raising survival rate of root and pseudobulb.Coconut palm chaff unit weight is small, water conservation
Property it is good, while adequate water supply is ensured, and can breathe freely.It makes containing large amount of organic matter in red soil, sawdust etc., is white
And growth provides suitable growing environment and sufficient nutrition；The present invention is using coconut palm in second of thermophilic hardening (transplanting later stage)
Largely using field original soil on the basis of chaff, crop field environment is conveniently adapted to, with the use of the dregs of a decoction, is on the one hand carried using preparing plant
Liquid is taken to generate waste dregs generated as raw material, waste utilization, the another aspect dregs of a decoction can effectively play sterilization in matrix to be pressed down
The effect of bacterium, hardening later stage need not can according to circumstances spray fungicide.
11st, the present invention can be using plant extraction liquid replacement carbendazim, in the aloe-emodin, garlic wherein in aloe
The ingredients such as the resveratrol in allicin and mulberries, giant knotweed, effective antibacterial bacteriostatic, by using plant extraction liquid, not only effectively
Original chemical pesticide is avoided to seriously endanger caused by water resource and soil, and have effects that good.
Specific embodiment
With reference to embodiment, the present invention is further illustrated, but the present invention is not limited in any way,
Based on present invention teach that any transformation or replacement made, all belong to the scope of protection of the present invention.
Embodiment 1
A kind of field production method of pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling, it is characterised in that include the following steps：
1) selection of land：Select warm moist, soil layer it is deep, it is fertile it is loose, draining is good, the red soil rich in organic matter, height above sea level
1600~2500m；
2) site preparation：30cm first is ploughed deeply into the plot chosen, then hack, then exposure 5 days, then to apply farm manure organic to soil
Matter content is not less than 3%, finally covers solar heat protection mulch to soil, carries out moise-heat sterilization, and retains covering with ground sheeting to before being colonized 2
My god, remove mulch, you can；
3) 30% shading net that height is 2m is built on plantation plot, and the height under shading net from earth's surface 1.6m sets up energy
The spray irrigation system in enough entire plantation plot of covering；
4) hardening：Selection September starts hardening, including hardening outside in-bottle seeding, cleaning and dipping, bottle；
5) it transplants：It first applies fertilizer to the subsoil, then builds out high 15cm, wide 100cm, moisture surface of the ditch heart away from 140cm, then by the purple Jing Guo hardening
Flower trident Herba Phaii tankervilliaes tissue-cultured seedling is according in the seeding row spacing field planting to the moisture surface of 20cm × 20cm；
6) field management：Periodically plot is irrigated using spray irrigation system, keeps soil moisture content 40%；First after transplanting
Year, every 2 months, 100kg/ mus of farm manure is applied, after transplanting 1 year, at quarterly intervals, 100kg/ mus of farm manure is applied and pours and apply biogas slurry
80kg。
Embodiment 2
A kind of field production method of pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling, it is characterised in that include the following steps：
1) selection of land：Select warm moist, soil layer it is deep, it is fertile it is loose, draining is good, the red soil rich in organic matter, height above sea level
2500m；
2) site preparation：50cm first is ploughed deeply into the plot chosen, then hack, then exposure 8 days, then to apply farm manure organic to soil
Matter content be not less than 3%, finally to soil cover solar heat protection mulch, carry out moise-heat sterilization, and retain covering with ground sheeting to be colonized before 2~
5 days, remove mulch, you can；
3) 50% shading net that height is 3m is built on plantation plot, and the height under shading net from earth's surface 1.8m sets up energy
The spray irrigation system in enough entire plantation plot of covering；
4) hardening：Select start hardening October, including hardening outside in-bottle seeding, cleaning and dipping, bottle；
5) it transplants：It first applies fertilizer to the subsoil, then builds out high 25cm, wide 120cm, moisture surface of the ditch heart away from 160cm, then by the purple Jing Guo hardening
Flower trident Herba Phaii tankervilliaes tissue-cultured seedling is according in the seeding row spacing field planting to the moisture surface of 20cm × 20cm；
6) field management：Periodically plot is irrigated using spray irrigation system, keeps soil moisture content 55%；First after transplanting
Year, at quarterly intervals, 200kg/ mus of farm manure is applied, after transplanting 1 year, every 4 months, apply 200kg/ mus of farm manure and pour and apply biogas slurry
100kg。
Embodiment 3
A kind of field production method of pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling, it is characterised in that include the following steps：
1) selection of land：Select warm moist, soil layer it is deep, it is fertile it is loose, draining is good, the red soil rich in organic matter, height above sea level
1600~1800m；
2) site preparation：35cm first is ploughed deeply into the plot chosen, then hack, then exposure 6 days, then apply well-rotted farmyard manure to soil
The content of organic matter is not less than 3%, finally covers solar heat protection mulch to soil, carries out moise-heat sterilization, and retains covering with ground sheeting to field planting
First 3 days, remove mulch, you can；The hack is that soil is broken to 5~2mm20% of soil particle, 2~0.2mm50%, 0.2
~0.02mm30%.
3) 40% shading net that height is 2.5m, and the height under shading net from earth's surface 1.7m are built on plantation plot
The spray irrigation system in entire plantation plot can be covered by setting up；
4) hardening：Selection starts hardening in 2 months, including hardening outside in-bottle seeding, cleaning and dipping, bottle；
The in-bottle seeding is the sterilization processing that first carries out disinfection to culturing room, then by pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling
Culture bottle lid is gradually taken off in culturing room, take off lid within 3 days and finish, during which environmental condition during tissue culture with being consistent；It then will training
Foster bottle is transferred to the position of the outer hardening of bottle, keeps ventilation, controls more than 85% shading, while it is 21 DEG C to keep temperature, and air is wet
It is more than 86% to spend, transition culture 6 days；The disinfection processing is that 1100 μ W/cm2 ultraviolet lightings are used in culturing room
Penetrate disinfection 70min.
The cleaning and dipping is divided into single plant and is placed on for pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling is taken out from culture bottle
In the culture dish of room temperature clear water equipped with 1cm depths, 2h is impregnated, it is then repeated as above to change water and soak 4 times, after changing water for the last time,
1.5 days growth-promoting roots are persistently impregnated, seedling is then impregnated into 15min using plant extraction liquid.
Then the preparation method of the plant extraction liquid first adds in 6 times of weights for aloe, garlic, mulberries, giant knotweed is taken to smash to pieces
, mistake complete to ethyl alcohol volatilization is boiled in the hot ethanol reflux 15min extractions of amount, the boiling that 8 times of weight are then added in into extraction vessel
Filter, obtains the dregs of a decoction and filtrate, filtrate is placed to room temperature, you can.
The outer hardening of the bottle includes the following steps：
A, first time thermophilic hardening：Bletilla tissue culture rooted seedling Jing Guo cleaning and dipping is colonized in the matrix of the first hardening bed,
The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of coconut palm chaff again, and cladding thickness 1cm keeps logical
Wind controls more than 80% shading, while it is 20 DEG C to keep temperature, and air humidity 80%, water content of substrate keeps more than 70%,
Hardening 15 days；
B, low temperature hardening：Ventilation is kept, controls more than 75% shading, while it is 17 DEG C to keep temperature, air humidity 80%, base
Matter water content keeps more than 65%, carries out first low temperature hardening 10 days；Ventilation is kept, more than 75% shading is controlled, keeps simultaneously
Temperature is 14 DEG C, and air humidity 80%, water content of substrate keeps more than 65%, carries out low temperature hardening 10 days again；
C, second of thermophilic hardening：Bletilla tissue culture Jing Guo low temperature hardening is taken root into transplantation of seedlings in the matrix of the second hardening bed,
The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of crop field original soil again, and cladding thickness 3cm is kept
Ventilation controls more than 70% shading, while it is 21 DEG C to keep temperature, and air humidity 72%, water content of substrate is gradually down to
More than 60%, hardening 18 days, you can；
D, room temperature hardening：Ventilation is kept, controls more than 70% shading, while temperature is controlled to be consistent with crop field temperature, air
More than 70% humidity, more than 60% water content of substrate, hardening 22 days, you can into field planting.
The matrix is to make red soil or containing the mixed-matrix for making red soil；The red soil of making refers to not argillaceous
Grain or clay particle content are less than 1% red soil.
5) it transplants：It first applies fertilizer to the subsoil, then builds out high 18cm, wide 105cm, moisture surface of the ditch heart away from 145cm, then hardening will be passed through
Pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling according to the seeding row spacing field planting of 20cm × 20cm in the moisture surface；It is described apply fertilizer to the subsoil for
110kg calcium magnesium phosphate is uniformly spread to plantation plot surface.
6) field management：Periodically plot is irrigated using spray irrigation system, keeps soil moisture content 45%；After transplanting
1 year, every 2.5 months, 160kg/ mus of farm manure is applied, after transplanting 1 year, at quarterly intervals, 110kg/ mus of farm manure is applied and pours and apply
Biogas slurry 90kg.
Embodiment 4
A kind of field production method of pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling, it is characterised in that include the following steps：
1) selection of land：Select warm moist, soil layer it is deep, it is fertile it is loose, draining is good, the red soil rich in organic matter, height above sea level
2000~2200m；
2) site preparation：40cm first is ploughed deeply into the plot chosen, then hack, then exposure 7 days, then to apply farm manure organic to soil
Matter content is not less than 3%, finally covers solar heat protection mulch to soil, carries out moise-heat sterilization, and retains covering with ground sheeting to before being colonized 2
My god, remove mulch, you can；The hack be by soil be broken to 5~2mm20% of soil particle, 2~0.2mm50%, 0.2~
0.02mm30%.
3) 40% shading net that height is 2.8m, and the height under shading net from earth's surface 1.8m are built on plantation plot
The spray irrigation system in entire plantation plot can be covered by setting up；
4) hardening：Select start hardening March, including hardening outside in-bottle seeding, cleaning and dipping, bottle；
The in-bottle seeding is the sterilization processing that first carries out disinfection to culturing room, then by pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling
Culture bottle lid is gradually taken off in culturing room, take off lid within 3 days and finish, during which environmental condition during tissue culture with being consistent；It then will training
Foster bottle is transferred to the position of the outer hardening of bottle, keeps ventilation, controls more than 85% shading, while it is 22 DEG C to keep temperature, and air is wet
It is more than 85% to spend, transition culture 7 days；The disinfection processing is that 1200 μ W/cm2 ultraviolet lightings are used in culturing room
Penetrate disinfection 120min.
The cleaning and dipping is divided into single plant and is placed on for pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling is taken out from culture bottle
In the culture dish of room temperature clear water equipped with 2cm depths, 3h is impregnated, it is then repeated as above to change water and soak 6 times, after changing water for the last time,
2 days growth-promoting roots are persistently impregnated, seedling is then impregnated into 20min using plant extraction liquid.
Then the preparation method of the plant extraction liquid first adds in 8 times of weights for aloe, garlic, mulberries, giant knotweed is taken to smash to pieces
, mistake complete to ethyl alcohol volatilization is boiled in the hot ethanol reflux 30min extractions of amount, the boiling that 12 times of weight are then added in into extraction vessel
Filter, obtains the dregs of a decoction and filtrate, filtrate is placed to room temperature, you can.
The outer hardening of the bottle includes the following steps：
A, first time thermophilic hardening：Bletilla tissue culture rooted seedling Jing Guo cleaning and dipping is colonized in the matrix of the first hardening bed,
The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of coconut palm chaff again, and cladding thickness 3cm keeps logical
Wind controls more than 80% shading, while it is 22 DEG C to keep temperature, and air humidity 85%, water content of substrate keeps more than 70%,
Hardening 30 days；
B, low temperature hardening：Ventilation is kept, controls more than 75% shading, while it is 19 DEG C to keep temperature, air humidity 82%, base
Matter water content keeps more than 65%, carries out first low temperature hardening 15 days；Ventilation is kept, more than 75% shading is controlled, keeps simultaneously
Temperature is 16 DEG C, and air humidity 82%, water content of substrate keeps more than 65%, carries out low temperature hardening 14 days again；
C, second of thermophilic hardening：Bletilla tissue culture Jing Guo low temperature hardening is taken root into transplantation of seedlings in the matrix of the second hardening bed,
The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of crop field original soil again, and cladding thickness 4cm is kept
Ventilation controls more than 70% shading, while it is 22 DEG C to keep temperature, and air humidity 78%, water content of substrate is gradually down to
More than 61%, hardening 20 days, you can；
D, room temperature hardening：Ventilation is kept, controls more than 71% shading, while temperature is controlled to be consistent with crop field temperature, air
More than 71% humidity, more than 61% water content of substrate, hardening 24 days, you can into field planting.
The matrix of the first hardening bed includes the ingredient of following parts by weight：15 parts of coconut palm chaff makes 8 parts of red soil, sawdust 6
Part, 6 parts of vermiculite, 6 parts of fertile soil.
The matrix of the second hardening bed includes the ingredient of following parts by weight：20 parts of field original soil, 10 parts of coconut palm chaff, the dregs of a decoction
5 parts；The dregs of a decoction are the extraction dregs of a decoction of aloe, garlic, mulberries and giant knotweed.
5) it transplants：It first applies fertilizer to the subsoil, then builds out high 20cm, wide 110cm, moisture surface of the ditch heart away from 150cm, then hardening will be passed through
Pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling according to the seeding row spacing field planting of 20cm × 20cm in the moisture surface；It is described apply fertilizer to the subsoil for
150kg calcium magnesium phosphate is uniformly spread to plantation plot surface.
6) field management：Periodically plot is irrigated using spray irrigation system, keeps soil moisture content 50%；After transplanting
1 year, every 2 months, 180kg/ mus of farm manure is applied, after transplanting 1 year, every 4 months, 150kg/ mus of farm manure is applied and pours and apply natural pond
Liquid 80kg.
Embodiment 5
A kind of field production method of pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling, it is characterised in that include the following steps：
1) selection of land：Select warm moist, soil layer it is deep, it is fertile it is loose, draining is good, the red soil rich in organic matter, height above sea level
2200~2400m；
2) site preparation：45cm first is ploughed deeply into the plot chosen, then hack, then exposure 8 days, then apply well-rotted farmyard manure to soil
The content of organic matter is not less than 3%, finally covers solar heat protection mulch to soil, carries out moise-heat sterilization, and retains covering with ground sheeting to field planting
First 4 days, remove mulch, you can；The hack is that soil is broken to 5~2mm20% of soil particle, 2~0.2mm50%, 0.2
~0.02mm30%.
3) 45% shading net that height is 2.7m, and the height under shading net from earth's surface 1.8m are built on plantation plot
The spray irrigation system in entire plantation plot can be covered by setting up；
4) hardening：Selection early September starts hardening, including hardening outside in-bottle seeding, cleaning and dipping, bottle；
The in-bottle seeding is the sterilization processing that first carries out disinfection to culturing room, then by pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling
Culture bottle lid is gradually taken off in culturing room, take off lid within 2.5 days and finish, during which environmental condition during tissue culture with being consistent；Then will
Culture bottle is transferred to the position of the outer hardening of bottle, keeps ventilation, controls more than 86% shading, while it is 20 DEG C to keep temperature, air
Humidity is more than 86%, transition culture 6 days；The disinfection processing is that 1050 μ W/cm2 ultraviolet lights are used in culturing room
Illumination-based disinfection 90min.
The cleaning and dipping is divided into single plant and is placed on for pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling is taken out from culture bottle
In the culture dish of room temperature clear water equipped with 1.5cm depths, 2.5h is impregnated, it is then repeated as above to change water and soak 5 times, water is changed for the last time
Afterwards, 2 days growth-promoting roots are persistently impregnated, seedling is then impregnated into 18min using plant extraction liquid.
Then the preparation method of the plant extraction liquid first adds in 7 times of weights for aloe, garlic, mulberries, giant knotweed is taken to smash to pieces
, mistake complete to ethyl alcohol volatilization is boiled in the hot ethanol reflux 28min extractions of amount, the boiling that 11 times of weight are then added in into extraction vessel
Filter, obtains the dregs of a decoction and filtrate, filtrate is placed to room temperature, you can.
The outer hardening of the bottle includes the following steps：
A, first time thermophilic hardening：Bletilla tissue culture rooted seedling Jing Guo cleaning and dipping is colonized in the matrix of the first hardening bed,
The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of coconut palm chaff again, and cladding thickness 2.5cm keeps logical
Wind controls more than 82% shading, while it is 21 DEG C to keep temperature, and air humidity 84%, water content of substrate keeps more than 72%,
Hardening 25 days；
B, low temperature hardening：Ventilation is kept, controls more than 77% shading, while it is 19 DEG C to keep temperature, air humidity 82%, base
Matter water content keeps more than 67%, carries out first low temperature hardening 14 days；Ventilation is kept, more than 77% shading is controlled, keeps simultaneously
Temperature is 16 DEG C, and air humidity 80%, water content of substrate keeps more than 67%, carries out low temperature hardening 13 days again；
C, second of thermophilic hardening：Bletilla tissue culture Jing Guo low temperature hardening is taken root into transplantation of seedlings in the matrix of the second hardening bed,
The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of crop field original soil again, and cladding thickness 4cm is kept
Ventilation controls more than 77% shading, while it is 20 DEG C to keep temperature, and air humidity 76%, water content of substrate is gradually down to
More than 63%, hardening 25 days, you can；
D, room temperature hardening：Ventilation is kept, controls more than 73% shading, while temperature is controlled to be consistent with crop field temperature, air
More than 73% humidity, more than 63% water content of substrate, hardening 24 days, you can into field planting.
The matrix of the first hardening bed includes the ingredient of following parts by weight：20 parts of coconut palm chaff makes 12 parts of red soil, sawdust
10 parts, 10 parts of vermiculite, 10 parts of fertile soil.
The matrix of the second hardening bed includes the ingredient of following parts by weight：20 parts of field original soil, 10 parts of coconut palm chaff, the dregs of a decoction
5 parts；The dregs of a decoction are the extraction dregs of a decoction of aloe, garlic, mulberries and giant knotweed.
5) it transplants：It first applies fertilizer to the subsoil, then builds out high 23cm, wide 115cm, moisture surface of the ditch heart away from 145cm, then hardening will be passed through
Pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling according to the seeding row spacing field planting of 20cm × 20cm in the moisture surface；It is described apply fertilizer to the subsoil for
145kg calcium magnesium phosphate is uniformly spread to plantation plot surface.
6) field management：Periodically plot is irrigated using spray irrigation system, keeps soil moisture content 50%；After transplanting
1 year, at quarterly intervals, 150kg/ mus of farm manure is applied, after transplanting 1 year, every 4 months, apply 120kg/ mus of farm manure and pour and apply natural pond
Liquid 90kg.
Embodiment 6
A kind of field production method of pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling, it is characterised in that include the following steps：
1) selection of land：Select warm moist, soil layer it is deep, it is fertile it is loose, draining is good, the red soil rich in organic matter, height above sea level
1700~1900m；
2) site preparation：48cm first is ploughed deeply into the plot chosen, then hack, then exposure 5~8 days, then apply farm manure to soil
The content of organic matter is not less than 3%, finally covers solar heat protection mulch to soil, carries out moise-heat sterilization, and retains covering with ground sheeting to field planting
First 5 days, remove mulch, you can；The hack is that soil is broken to 5~2mm20% of soil particle, 2~0.2mm50%, 0.2
~0.02mm30%.
3) 30% shading net that height is 3m, and the height frame under shading net from earth's surface 1.6m are built on plantation plot
If the spray irrigation system in entire plantation plot can be covered；
4) hardening：Selection late October starts hardening, including hardening outside in-bottle seeding, cleaning and dipping, bottle；
The in-bottle seeding is the sterilization processing that first carries out disinfection to culturing room, then by pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling
Culture bottle lid is gradually taken off in culturing room, take off lid within 2 days and finish, during which environmental condition during tissue culture with being consistent；It then will training
Foster bottle is transferred to the position of the outer hardening of bottle, keeps ventilation, controls more than 85% shading, while it is 22 DEG C to keep temperature, and air is wet
It is more than 85% to spend, transition culture 5 days；The disinfection processing is that 1200 μ W/cm2 ultraviolet lightings are used in culturing room
Penetrate disinfection 85min.
The cleaning and dipping is divided into single plant and is placed on for pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling is taken out from culture bottle
In the culture dish of room temperature clear water equipped with 1.2cm depths, 2h is impregnated, it is then repeated as above to change water and soak 5 times, water is changed for the last time
Afterwards, 1 day growth-promoting root is persistently impregnated, seedling is then impregnated into 16min using plant extraction liquid.
Then the preparation method of the plant extraction liquid first adds in 7 times of weights for aloe, garlic, mulberries, giant knotweed is taken to smash to pieces
, mistake complete to ethyl alcohol volatilization is boiled in the hot ethanol reflux 22min extractions of amount, the boiling that 11 times of weight are then added in into extraction vessel
Filter, obtains the dregs of a decoction and filtrate, filtrate is placed to room temperature, you can.
The outer hardening of the bottle includes the following steps：
A, first time thermophilic hardening：Bletilla tissue culture rooted seedling Jing Guo cleaning and dipping is colonized in the matrix of the first hardening bed,
The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of coconut palm chaff again, and cladding thickness 2cm keeps logical
Wind controls more than 80% shading, while it is 21 DEG C to keep temperature, and air humidity 82%, water content of substrate keeps more than 70%,
Hardening 15~30 days；
B, low temperature hardening：Ventilation is kept, controls more than 75% shading, while it is 17~19 DEG C to keep temperature, air humidity is
80%, water content of substrate keeps more than 65%, carries out first low temperature hardening 11 days；Ventilation is kept, controls more than 79% shading, together
When to keep temperature be 15 DEG C, air humidity 82%, water content of substrate keeps more than 69%, carries out low temperature hardening 15 days again；
C, second of thermophilic hardening：Bletilla tissue culture Jing Guo low temperature hardening is taken root into transplantation of seedlings in the matrix of the second hardening bed,
The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of crop field original soil again, and cladding thickness 3cm is kept
Ventilation controls more than 76% shading, while it is 20 DEG C to keep temperature, and air humidity 79%, water content of substrate is gradually down to
More than 64%, hardening 15~30 days, you can；
D, room temperature hardening：Ventilation is kept, controls more than 74% shading, while temperature is controlled to be consistent with crop field temperature, air
More than 74% humidity, more than 64% water content of substrate, hardening 21 days, you can into field planting.
The matrix of the first hardening bed includes the ingredient of following parts by weight：18 parts of coconut palm chaff makes 10 parts of red soil, sawdust
8 parts, 8 parts of vermiculite, 8 parts of fertile soil.
The matrix of the second hardening bed includes the ingredient of following parts by weight：19 parts of field original soil, 9 parts of coconut palm chaff, the dregs of a decoction 4
Part；The dregs of a decoction are the extraction dregs of a decoction of aloe, garlic, mulberries and giant knotweed.
5) it transplants：It first applies fertilizer to the subsoil, then builds out high 20cm, wide 110cm, moisture surface of the ditch heart away from 150cm, then hardening will be passed through
Pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling according to the seeding row spacing field planting of 20cm × 20cm in the moisture surface；It is described apply fertilizer to the subsoil for
140kg calcium magnesium phosphate is uniformly spread to plantation plot surface.
6) field management：Periodically plot is irrigated using spray irrigation system, keeps soil moisture content 45%；After transplanting
1 year, at quarterly intervals, 120kg/ mus of farm manure is applied, after transplanting 1 year, at quarterly intervals, apply 180kg/ mus of farm manure and pour and apply natural pond
Liquid 85kg.
Claims (10)
1. a kind of field production method of pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling, it is characterised in that include the following steps：
1) selection of land：Select warm moist, soil layer it is deep, it is fertile it is loose, draining is good, the red soil rich in organic matter, height above sea level
1600~2500m；
2) site preparation：30~50cm first is ploughed deeply into the plot chosen, then hack, then exposure 5~8 days, then apply decomposed farmers'
Fertilizer, not less than 3%, finally covers solar heat protection mulch to soil organic matter content to soil, carries out moise-heat sterilization, and retain mulch and cover
Lid removes mulch to before being colonized 2~5 days, you can；
3) 30~50% shading nets that height is 2~3m are built on plantation plot, and from 1.6~1.8m of earth's surface under shading net
Height erection can cover the spray irrigation system in entire plantation plot；
4) hardening：Select start hardening to March September to October or 2 months, including hardening outside in-bottle seeding, cleaning and dipping, bottle；
5) it transplants：It first applies fertilizer to the subsoil, then builds out high 15~25cm, wide 100~120cm, moisture surface of the ditch heart away from 140~160cm, then
By the pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling Jing Guo hardening according in the seeding row spacing field planting to the moisture surface of 20cm × 20cm；
6) field management：Periodically plot is irrigated using spray irrigation system, keeps soil moisture content 40%~55%；After transplanting
First Year every 2~3 months, is applied 100~200kg/ mus of well-rotted farmyard manure, after transplanting 1 year, every 3~4 months, is applied decomposed
100~200kg/ mus of farm manure simultaneously pours and applies 80~100kg of biogas slurry.
2. the field production method of pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling according to claim 1, it is characterised in that step (2)
Described in hack be that soil is broken to 5~2mm20% of soil particle, 2~0.2mm50%, 0.2~0.02mm30%.
3. the field production method of pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling according to claim 1, it is characterised in that step (4)
Described in in-bottle seeding first to carry out disinfection to culturing room sterilization processing, then by pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling
Culture bottle gradually takes off lid in culturing room, takes off lid within 2~3 days and finishes, during which environmental condition during tissue culture with being consistent；It then will training
Foster bottle is transferred to the position of the outer hardening of bottle, keeps ventilation, controls more than 85% shading, while it is 20~22 DEG C to keep temperature, empty
Air humidity degree is more than 85%, transition culture 5~7 days；The disinfection processing is that 1000~1200 μ are used in culturing room
W/cm2Ultraviolet light sterilizes 60~120min.
4. the field production method of pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling according to claim 1, it is characterised in that step (4)
Described in cleaning and dipping for pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling is taken out from culture bottle, be divided into single plant and be placed on and be equipped with
In the culture dish of the room temperature clear water of 0.5~2cm depths, 1~3h is impregnated, then repeated as above to change water and soak 3~6 times, last time
After changing water, 1~2 day growth-promoting root is persistently impregnated, seedling is then impregnated into 10~20min using plant extraction liquid.
5. the field production method of pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling according to claim 4, it is characterised in that described
Then the preparation method of plant extraction liquid first adds in the hot ethanol of 6~8 times of weight for aloe, garlic, mulberries, giant knotweed is taken to smash to pieces
Flow back 15~30min extractions, and, filtering complete to ethyl alcohol volatilization is boiled in the boiling that 8~12 times of weight are then added in into extraction vessel,
The dregs of a decoction and filtrate are obtained, filtrate is placed to room temperature, you can.
6. the field production method of pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling according to claim 1, it is characterised in that step (4)
Described in the outer hardening of bottle include the following steps：
A, first time thermophilic hardening：Bletilla tissue culture rooted seedling Jing Guo cleaning and dipping is colonized in the matrix of the first hardening bed,
The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of coconut palm chaff again, and cladding thickness is 1~3cm, is kept
Ventilation controls more than 80% shading, while it is 20~22 DEG C to keep temperature, and air humidity is 80~85%, and water content of substrate is protected
More than 70% is held, hardening 15~30 days；
B, low temperature hardening：Ventilation is kept, controls more than 75% shading, while it is 17~19 DEG C to keep temperature, air humidity is
80%~82%, water content of substrate keeps more than 65%, carries out first low temperature hardening 10~15 days；Ventilation is kept, controls shading
More than 75%, while it is 14~16 DEG C to keep temperature, air humidity is 80%~82%, and water content of substrate keeps more than 65%,
Carry out low temperature hardening 10~15 days again；
C, second of thermophilic hardening：Bletilla tissue culture Jing Guo low temperature hardening is taken root into transplantation of seedlings in the matrix of the second hardening bed,
The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of crop field original soil again, and cladding thickness is 2~4cm,
Ventilation is kept, controls more than 70% shading, while it is 20~22 DEG C to keep temperature, air humidity is 70~80%, and matrix is aqueous
Amount is gradually down to more than 60%, hardening 15~30 days, you can；
D, room temperature hardening：Ventilation is kept, controls more than 70% shading, while temperature is controlled to be consistent with crop field temperature, air
More than 70% humidity, more than 60% water content of substrate, hardening 20~30 days, you can into field planting.
7. the field production method of pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling according to claim 6, it is characterised in that described
Matrix is to make red soil or containing the mixed-matrix for making red soil；The red soil of making refers to without clay particle or clay particle content
Red soil less than 1%.
8. the acclimatization and transplants method of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling according to claim 6, it is characterised in that institute
The matrix of the first hardening bed stated includes the ingredient of following parts by weight：15~20 parts of coconut palm chaff, make 8~12 parts of red soil, sawdust 6~
10 parts, 6~10 parts of vermiculite, 6~10 parts of fertile soil.
9. the acclimatization and transplants method of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling according to claim 6, it is characterised in that institute
The matrix of the second hardening bed stated includes the ingredient of following parts by weight：15~20 parts of field original soil, 6~10 parts of coconut palm chaff, the dregs of a decoction 3~
5 parts；The dregs of a decoction are the extraction dregs of a decoction of aloe, garlic, mulberries and giant knotweed.
10. the field production method of pale reddish brown trident Herba Phaii tankervilliaes tissue-cultured seedling according to claim 1, it is characterised in that step
(5) applying fertilizer to the subsoil as 100~150kg calcium magnesium phosphate to be uniformly spread to plantation plot surface described in.
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| CN109169132A (en) * | 2018-09-27 | 2019-01-11 | 岭南生态文旅股份有限公司 | A kind of Hipeastrum vittalum (L Her.) Herb.- Amaryllisvittata Ait tissue-cultured seedling crop field efficient cultivation method |
| CN112997623A (en) * | 2021-01-26 | 2021-06-22 | 贵州丰萃农业发展有限公司 | High-density vegetable planting pre-culture weeding technology |
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