CN108157098A - A kind of acclimatization and transplants method of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling - Google Patents
A kind of acclimatization and transplants method of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling Download PDFInfo
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- CN108157098A CN108157098A CN201810059164.XA CN201810059164A CN108157098A CN 108157098 A CN108157098 A CN 108157098A CN 201810059164 A CN201810059164 A CN 201810059164A CN 108157098 A CN108157098 A CN 108157098A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
Abstract
The invention discloses a kind of acclimatization and transplants methods of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling, include the following steps：1) in-bottle seeding；2) cleaning and dipping；3) the outer hardening of bottle：A, first time thermophilic hardening；B, low temperature hardening；C, second of thermophilic hardening；D, room temperature hardening.The present invention greatly reduces miscellaneous bacteria and tissue-cultured seedling is infected, and improves survival rate, is conducive to cultivate health seedling, significant to improving field planting survival rate and subsequent growth, so as to greatly improve product quality and yield lays the first stone.Hardening stage by stage of the invention, has the more scientific laundering period, greatly improves the survival rate of transplanting, shortens transplanting seedling time；The hardening survival rate of the present invention can reach more than 99%.
Description
Technical field
The invention belongs to bletilla technical field of cultivation, and in particular to a kind of hardening of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling
Method for transplanting.
Background technology
Pale reddish brown trident Herba Phaii tankervilliaes are one of orchid bletilla Bletilla striata (Thunb.) Reichb.f.
Kind, it is other to also have chrysanthemum, white flower, light red flower etc. not of the same race, also distinguished with the small bletilla of pale reddish brown trident in Yunnan.Bletilla is sent out at present
Existing has 6 kinds, and China has 4 kinds, and application that can be different degrees of is in medicine.But medicinal material of the highest excellence belongs to pale reddish brown three
Bletilla (containing pale reddish brown trident Herba Phaii tankervilliaes and the small bletilla of pale reddish brown trident) is pitched, is loaded into the Bletilla striata medicinal materials kind of version Chinese Pharmacopoeia in 2015 just
Two conditions are described in detail, when purple flower, second is that medicinal material stem tuber will have 2~3 claw-like branches, long 1.5~5cm, thickness 0.5
~1.5cm.Either pattern is not purple or flower for purple and does not have claw-like branch most bletilla kinds.Therefore into pedestrian
When work is cultivated, the requirement for selecting pale reddish brown trident bletilla that could meet pharmacopeia is had to.
Bletilla striata medicinal materials are always by excavation wild resource, and with the exhaustion of resource, it is wild to be included in focused protection by country
Plant.People begin attempt to artificial growth bletilla, it is existing have planted using stem tuber and using Plant Tissue Breeding
Technology is planted to breed bletilla seedling, but training tissue culture seedling cultivation technique is also immature, and hardening is bletilla tissue-cultured seedling input
To the premise of field production, different hardening off method has large effect to the survival rate of hardening, the robustness of seedling, existing
Hardening off method not only transplanting survival rate is low and the period is long, it has also become the bottleneck of bletilla industrialization.Tissue-cultured seedling from sterile heterotrophism to
Outer this section of transplanting domestication for having bacterium autotrophy of bottle, is very easy to a large amount of dead seedlings；Or the seedling brought out is of low quality, field planting effect
The problems such as fruit difference etc..During conventional hardening, often using modes such as strong light hardening, Small plastic shed hardenings, acclimatization and transplants survival rate is about
60% or so, the seedling-slowing stage time is long, high by root rot, stem rot infection probability.
Therefore, it is necessary to invent a kind of acclimatization and transplants method of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling.
Invention content
The purpose of the present invention is to provide a kind of acclimatization and transplants methods of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling.
The object of the present invention is achieved like this, includes the following steps：
1) in-bottle seeding：Select start hardening to March September to October or 2 months, first carry out disinfection to culturing room sterilization processing, so
The culture bottle of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling in culturing room is gradually taken off into lid afterwards, lid is taken off within 2~3 days and finishes, during which ring
Border condition during tissue culture with being consistent；Culture bottle is then transferred to the position of hardening outside bottle, keeps ventilation, controls shading 85%
More than, while it is 20~22 DEG C to keep temperature, air humidity is more than 85%, transition culture 5~7 days；
2) cleaning and dipping：It will be taken out from culture bottle by the pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling of in-bottle seeding, be divided into list
Strain is placed in the culture dish of the room temperature clear water equipped with 0.5~2cm depth, 1~3h of immersion, then repeats to change water and soak 3~6 times, most
After once changing water afterwards, 1~2 day growth-promoting root is persistently impregnated, seedling is then impregnated 10 using 800~1000 times of carbendazols~
20min；
3) the outer hardening of bottle：
A, first time thermophilic hardening：Bletilla tissue culture rooted seedling Jing Guo cleaning and dipping is colonized in the matrix of the first hardening bed,
The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of coconut palm chaff again, and cladding thickness is 1~3cm, is kept
Ventilation controls more than 80% shading, while it is 20~22 DEG C to keep temperature, and air humidity is 80~85%, and water content of substrate is protected
More than 70% is held, hardening 15~30 days；
B, low temperature hardening：Ventilation is kept, controls more than 75% shading, while it is 17~19 DEG C to keep temperature, air humidity is
80%~82%, water content of substrate keeps more than 65%, carries out first low temperature hardening 10~15 days；Ventilation is kept, controls shading
More than 75%, while it is 14~16 DEG C to keep temperature, air humidity is 80%~82%, and water content of substrate keeps more than 65%,
Carry out low temperature hardening 10~15 days again；
C, second of thermophilic hardening：Bletilla tissue culture Jing Guo low temperature hardening is taken root into transplantation of seedlings in the matrix of the second hardening bed,
The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of crop field original soil again, and cladding thickness is 2~4cm,
Ventilation is kept, controls more than 70% shading, while it is 20~22 DEG C to keep temperature, air humidity is 70~80%, and matrix is aqueous
Amount is gradually down to more than 60%, hardening 15~30 days；
D, room temperature hardening：Ventilation is kept, controls more than 70% shading, while temperature is controlled to be consistent with crop field temperature, air
More than 70% humidity, more than 60% water content of substrate, hardening 20~30 days, you can into field planting.
Preferably, the disinfection processing described in step (1) is purple using 1000~1200 μ W/cm2 in culturing room
Outer smooth 60~120min of illumination-based disinfection.
Preferably, 800~1000 times of carbendazols described in step (2) can be substituted using plant extraction liquid.
Preferably, then the preparation method of the plant extraction liquid first adds for aloe, garlic, mulberries, giant knotweed is taken to smash to pieces
Enter hot ethanol reflux 15~30min extractions of 6~8 times of weight, the boiling boiling of 8~12 times of weight is then added in into extraction vessel
Complete to ethyl alcohol volatilization, filtering obtains the dregs of a decoction and filtrate, filtrate is placed to room temperature to obtain the final product.
Preferably, the matrix described in step (3) is to make red soil or containing the mixed-matrix for making red soil.
The red soil made red soil and refer to be less than without clay particle or clay particle content 1%.
Preferably, the matrix of the first hardening bed described in step (3) includes the ingredient of following parts by weight：Coconut palm chaff 15~20
Part makes 8~12 parts of red soil, 6~10 parts of sawdust, 6~10 parts of vermiculite, 6~10 parts of fertile soil.
Preferably, the matrix of the second hardening bed described in step (3) includes the ingredient of following parts by weight：Field original soil 15
~20 parts, 6~10 parts of coconut palm chaff, 3~5 parts of the dregs of a decoction；The dregs of a decoction are the extraction dregs of a decoction of aloe, garlic, mulberries and giant knotweed.
Preferably, the prevention and control of plant diseases, pest control is further included in step (3)：Outside bottle during hardening, every 10~20 days, alternately
Spray the solution 1 time of 0.1% concentration of carbendazim or thiophanate methyl.
Preferably, top dressing is further included in step (3)：During second of thermophilic hardening and room temperature hardening, every 20
It is~30 days, primary using potassium dihydrogen phosphate aqueous solution foliage-spray.
More than 70% shading is since bletilla belongs to half shade plant, and shading is had in the training tissue culture seedling stage
More than 70% enters field planting until going out canopy.
Compared with prior art, beneficial effects of the present invention：
1st, the present invention greatly reduces miscellaneous bacteria and tissue-cultured seedling is infected, and improves the survival rate of tissue-cultured seedling, is conducive to cultivate health
Seedling, it is significant to improving field planting survival rate and subsequent growth, so as to greatly improve product quality and yield is beaten
Lower basis.
2nd, hardening stage by stage of the invention, has the more scientific laundering period, greatly improves the survival rate of transplanting, shortens
Transplanting seedling time；The hardening survival rate of the present invention can reach more than 99%, and field-transplanting survival rate can reach more than 98%.
3rd, the present invention passes through the temperature change of thermophilic-low temperature-thermophilic-room temperature during hardening, first passes through preference temperature
Hardening so that just contact external environment seedling can be adapted to smoothly under preference temperature, then using low temperature hardening, be improved kind
Seedling then proceedes to carry out hardening using preference temperature, will grow bad seedling at low temperature to the resistivity of adverse environment
Again strong sprout is developed into, it is consistent with transplanting ground temperature so as to fulfill hardening temperature finally using crop field temperature hardening, it greatly shortens
Raising survival rate.
4th, hardening selection of time science of the invention, no sprouting sprouts, therefore effectively reduces the feelings for hindering bud when washing seedling
Condition.Present invention selection carries out hardening in the rest period of bletilla, improves transplanting survival rate.The present invention is by the prevention and control of plant diseases, pest control and chases after
Fertilizer promotes bletilla strong sprout, further improves the survival rate of field-transplanting.
5th, the present invention is similar to environmental condition during culture at hardening initial stage by rationally carrying out hardening, avoids seedling first
Adverse conditions is grown into the environment to differ greatly, then carries out low temperature hardening, finally in the hardening later stage, with field-transplanting
Environmental condition it is similar, so as to achieve the purpose that gradually to adapt to.Present invention selection autumn and spring carry out hardening, avoid high temperature
The situation that seedling caused by high humidity weather is wilted；By the water supply in high humidity guarantee early period seedling, the later stage is gradually close to naturally wet
Degree so that seedling can adapt to and well-grown, improves survival rate.
6th, the present invention is by first to indoor carry out ultraviolet disinfection, eliminating miscellaneous bacteria that may be present in culturing room, avoiding group
Training seedling start contact external environment resistance it is not strong and may caused by disease, for continue hardening lay the first stone.Again by bottle
Transition culture certain time in bottle in the environment of outer culture, by optimum humiture, effectively prevent bletilla tissue-cultured seedling pair
Environment is not suitable for, so as to which it be helped smoothly to adapt to new environmental condition.The present invention is had safely using physical disinfection method
Effect, avoids the environmental problem that chemical disinfection method is brought.
7th, on the one hand the present invention changes water by tissue culture rooted seedling is impregnated directly in clear water by repeatedly impregnating, effectively clear
The culture medium in tissue-cultured seedling has been managed, has avoided a large amount of clear water of culture medium consuming for removing root in a short time, and easily cause
The problem of damage of cauline leaf and root, prior one side are can to effectively facilitate kind of a seedling rooting by repeatedly impregnating, and
A great number of elements, trace element in early period culture medium can be used as nutrient solution in clear water, provide nutrition progress for seedling is of short duration
Transition.
8th, the present invention using the coconut palm chaff of more water-permeable and air permeable, makes red soil, saw in first time thermophilic hardening (transplanting initial stage)
End etc., anti-ponding, loose ventilative, fertility is high, prevents rotten, the raising survival rate of root and pseudobulb.Coconut palm chaff unit weight is small, water-retaining property
It is good, while adequate water supply is ensured, and it can breathe freely.It makes containing large amount of organic matter in red soil, sawdust etc., is bletilla
Growth provides suitable growing environment and sufficient nutrition；The present invention is using coconut palm chaff in second of thermophilic hardening (transplanting later stage)
On the basis of largely using field original soil, crop field environment is conveniently adapted to, with the use of the dregs of a decoction, on the one hand using preparing plant extract
Liquid generates waste dregs generated as raw material, and waste utilization, the another aspect dregs of a decoction can effectively play bactericidal in matrix
Effect, the hardening later stage need not can according to circumstances spray fungicide.
9th, the present invention can be using plant extraction liquid replacement carbendazim, in the aloe-emodin, garlic wherein in aloe
The ingredients such as the resveratrol in allicin and mulberries, giant knotweed, effective antibacterial bacteriostatic, by using plant extraction liquid, not only effectively
Original chemical pesticide is avoided to seriously endanger caused by water resource and soil, and have effects that good.
Specific embodiment
With reference to case study on implementation, the present invention is further illustrated, but the present invention is not limited in any way
System, based on present invention teach that any transformation or replacement made, all belong to the scope of protection of the present invention.
Embodiment 1
A kind of acclimatization and transplants method of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling, includes the following steps：
1) in-bottle seeding：Selection September starts hardening, and first carry out disinfection to culturing room sterilization processing, then by pale reddish brown trident great Bai
And the culture bottle of tissue culture rooted seedling gradually takes off lid in culturing room, takes off lid within 2 days and finishes, during which environmental condition during tissue culture with keeping one
It causes；Culture bottle is then transferred to the position of hardening outside bottle, keeps ventilation, controls more than 85% shading, while keeps the temperature to be
20 DEG C, air humidity is more than 85%, transition culture 5 days；
2) cleaning and dipping：It will be taken out from culture bottle by the pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling of in-bottle seeding, be divided into list
Strain is placed in the culture dish of the room temperature clear water equipped with 0.5cm depths, is impregnated 1h, is then repeated to change water and soak 3 times, change water for the last time
Afterwards, 1 day growth-promoting root is persistently impregnated, seedling is then impregnated into 10min using 800 times of carbendazols；
3) the outer hardening of bottle：
A, first time thermophilic hardening：Bletilla tissue culture rooted seedling Jing Guo cleaning and dipping is colonized in the matrix of the first hardening bed,
The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of coconut palm chaff again, and cladding thickness 1cm keeps logical
Wind controls more than 80% shading, while it is 20 DEG C to keep temperature, and air humidity 80%, water content of substrate keeps more than 70%,
Hardening 15 days；
B, low temperature hardening：Ventilation is kept, controls more than 75% shading, while it is 17 DEG C to keep temperature, air humidity 80%, base
Matter water content keeps more than 65%, carries out first low temperature hardening 10 days；Ventilation is kept, more than 75% shading is controlled, keeps simultaneously
Temperature is 14 DEG C, and air humidity 80%, water content of substrate keeps more than 65%, carries out low temperature hardening 10 days again；
C, second of thermophilic hardening：Bletilla tissue culture Jing Guo low temperature hardening is taken root into transplantation of seedlings in the matrix of the second hardening bed,
The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of crop field original soil again, and cladding thickness 2cm is kept
Ventilation controls more than 70% shading, while it is 20 DEG C to keep temperature, and air humidity 70%, water content of substrate is gradually down to
More than 60%, hardening 15 days；
D, room temperature hardening：Ventilation is kept, controls more than 70% shading, while temperature is controlled to be consistent with crop field temperature, air
More than 70% humidity, more than 60% water content of substrate, hardening 20 days, you can into field planting.
Embodiment 2
A kind of acclimatization and transplants method of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling, includes the following steps：
1) in-bottle seeding：Select start hardening October, first carry out disinfection to culturing room sterilization processing, then by pale reddish brown trident great Bai
And the culture bottle of tissue culture rooted seedling gradually takes off lid in culturing room, takes off lid within 3 days and finishes, during which environmental condition during tissue culture with keeping one
It causes；Culture bottle is then transferred to the position of hardening outside bottle, keeps ventilation, controls more than 85% shading, while keeps the temperature to be
22 DEG C, air humidity is more than 85%, transition culture 7 days；
2) cleaning and dipping：It will be taken out from culture bottle by the pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling of in-bottle seeding, be divided into list
Strain is placed in the culture dish of the room temperature clear water equipped with 2cm depths, is impregnated 3h, is then repeated to change water and soak 6 times, change water for the last time
Afterwards, 2 days growth-promoting roots are persistently impregnated, seedling is then impregnated into 20min using 1000 times of carbendazols；
3) the outer hardening of bottle：
A, first time thermophilic hardening：Bletilla tissue culture rooted seedling Jing Guo cleaning and dipping is colonized in the matrix of the first hardening bed,
The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of coconut palm chaff again, and cladding thickness 3cm keeps logical
Wind controls more than 80% shading, while it is 22 DEG C to keep temperature, and air humidity 85%, water content of substrate keeps more than 70%,
Hardening 30 days；
B, low temperature hardening：Ventilation is kept, controls more than 75% shading, while it is 19 DEG C to keep temperature, air humidity 82%, base
Matter water content keeps more than 65%, carries out first low temperature hardening 15 days；Ventilation is kept, more than 75% shading is controlled, keeps simultaneously
Temperature is 16 DEG C, and air humidity 82%, water content of substrate keeps more than 65%, carries out low temperature hardening 15 days again；
C, second of thermophilic hardening：Bletilla tissue culture Jing Guo low temperature hardening is taken root into transplantation of seedlings in the matrix of the second hardening bed,
The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of crop field original soil again, and cladding thickness 4cm is kept
Ventilation controls more than 70% shading, while it is 22 DEG C to keep temperature, and air humidity 80%, water content of substrate is gradually down to
More than 60%, hardening 30 days；
D, room temperature hardening：Ventilation is kept, controls more than 70% shading, while temperature is controlled to be consistent with crop field temperature, air
More than 70% humidity, more than 60% water content of substrate, hardening 30 days, you can into field planting.
Embodiment 3
A kind of acclimatization and transplants method of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling, includes the following steps：
1) in-bottle seeding：Selection starts hardening in 2 months, and first carry out disinfection to culturing room sterilization processing, then by pale reddish brown trident great Bai
And the culture bottle of tissue culture rooted seedling gradually takes off lid in culturing room, takes off lid within 2 days and finishes, during which environmental condition during tissue culture with keeping one
It causes；Culture bottle is then transferred to the position of hardening outside bottle, keeps ventilation, controls more than 90% shading, while keeps the temperature to be
21 DEG C, air humidity is more than 90%, transition culture 6 days；The disinfection processing is that 1100 μ W/ are used in culturing room
Cm2 ultraviolet lights sterilize 90min；
2) cleaning and dipping：It will be taken out from culture bottle by the pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling of in-bottle seeding, be divided into list
Strain is placed in the culture dish of the room temperature clear water equipped with 1cm depths, is impregnated 2h, is then repeated to change water and soak 4 times, change water for the last time
Afterwards, 1.5 days growth-promoting roots are persistently impregnated, seedling is then impregnated into 15min using plant extraction liquid；The system of the plant extraction liquid
Then Preparation Method first adds in the hot ethanol reflux 15min extractions of 6 times of weight, then for aloe, garlic, mulberries, giant knotweed is taken to smash to pieces
The boiling that 8 times of weight are added in into extraction vessel is boiled to ethyl alcohol volatilization completely, and filtering obtains the dregs of a decoction and filtrate, filtrate is placed
To room temperature to obtain the final product；
3) the outer hardening of bottle：
A, first time thermophilic hardening：Bletilla tissue culture rooted seedling Jing Guo cleaning and dipping is colonized in the matrix of the first hardening bed,
The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of coconut palm chaff again, and cladding thickness 2cm keeps logical
Wind controls more than 85% shading, while it is 21 DEG C to keep temperature, and air humidity 82%, water content of substrate keeps more than 75%,
Hardening 20 days；
B, low temperature hardening：Ventilation is kept, controls more than 76% shading, while it is 18 DEG C to keep temperature, air humidity 81%, base
Matter water content keeps more than 66%, carries out first low temperature hardening 11 days；Ventilation is kept, more than 75% shading is controlled, keeps simultaneously
Temperature is 15 DEG C, and air humidity 81%, water content of substrate keeps more than 65%, carries out low temperature hardening 11 days again；
C, second of thermophilic hardening：Bletilla tissue culture Jing Guo low temperature hardening is taken root into transplantation of seedlings in the matrix of the second hardening bed,
The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of crop field original soil again, and cladding thickness 3cm is kept
Ventilation controls more than 71% shading, while it is 21 DEG C to keep temperature, and air humidity 72%, water content of substrate is gradually down to
More than 62%, hardening 20 days；
D, room temperature hardening：Ventilation is kept, controls more than 70% shading, while temperature is controlled to be consistent with crop field temperature, air
More than 71% humidity, more than 61% water content of substrate, hardening 22 days, you can into field planting.
Matrix described in step (3) is to make red soil or containing the mixed-matrix for making red soil；The red soil of making is
Refer to without the red soil of clay particle or clay particle content less than 1%.
Step further includes the prevention and control of plant diseases, pest control in (3)：Outside bottle during hardening, every 10 days, alternating sprayed carbendazim
Or the solution of 0.1% concentration of thiophanate methyl 1 time.
Step further includes top dressing in (3)：During second of thermophilic hardening and room temperature hardening, every 20 days, use
Potassium dihydrogen phosphate aqueous solution foliage-spray is primary.
Embodiment 4
A kind of acclimatization and transplants method of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling, includes the following steps：
1) in-bottle seeding：Select start hardening March, first carry out disinfection to culturing room sterilization processing, then by pale reddish brown trident great Bai
And the culture bottle of tissue culture rooted seedling gradually takes off lid in culturing room, takes off lid within 3 days and finishes, during which environmental condition during tissue culture with keeping one
It causes；Culture bottle is then transferred to the position of hardening outside bottle, keeps ventilation, controls more than 86% shading, while keeps the temperature to be
20 DEG C, air humidity is more than 86%, transition culture 5 days；The disinfection processing is that 1050 μ W/ are used in culturing room
Cm2 ultraviolet lights sterilize 70min；
2) cleaning and dipping：It will be taken out from culture bottle by the pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling of in-bottle seeding, be divided into list
Strain is placed in the culture dish of the room temperature clear water equipped with 1.2cm depths, is impregnated 1.5h, is then repeated to change water and soak 4 times, change for the last time
After water, 1 day growth-promoting root is persistently impregnated, seedling is then impregnated into 20min using plant extraction liquid；The system of the plant extraction liquid
Then Preparation Method first adds in the hot ethanol reflux 30min extractions of 8 times of weight, then for aloe, garlic, mulberries, giant knotweed is taken to smash to pieces
, filtering complete to ethyl alcohol volatilization is boiled in the boiling that 12 times of weight are added in into extraction vessel, obtains the dregs of a decoction (for the of the outer hardening of bottle
In the matrix of two hardening beds) and filtrate, filtrate is placed to room temperature to obtain the final product；
3) the outer hardening of bottle：
A, first time thermophilic hardening：Bletilla tissue culture rooted seedling Jing Guo cleaning and dipping is colonized in the matrix of the first hardening bed,
The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of coconut palm chaff again, and cladding thickness 3cm keeps logical
Wind controls more than 80% shading, while it is 22 DEG C to keep temperature, and air humidity 82%, water content of substrate keeps more than 72%,
Hardening 25 days；
B, low temperature hardening：Ventilation is kept, controls more than 77% shading, while it is 19 DEG C to keep temperature, air humidity 81%, base
Matter water content keeps more than 65%, carries out first low temperature hardening 14 days；Ventilation is kept, more than 75% shading is controlled, keeps simultaneously
Temperature is 16 DEG C, and air humidity 82%, water content of substrate keeps more than 65%, carries out low temperature hardening 13 days again；
C, second of thermophilic hardening：Bletilla tissue culture Jing Guo low temperature hardening is taken root into transplantation of seedlings in the matrix of the second hardening bed,
The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of crop field original soil again, and cladding thickness 2cm is kept
Ventilation controls more than 70% shading, while it is 20 DEG C to keep temperature, and air humidity 74%, water content of substrate is gradually down to
More than 62%, hardening 25 days；
D, room temperature hardening：Ventilation is kept, controls more than 70% shading, while temperature is controlled to be consistent with crop field temperature, air
More than 70% humidity, more than 60% water content of substrate, hardening 25 days, you can into field planting.
The matrix of the first hardening bed described in step (3) includes the ingredient of following parts by weight：15 parts of coconut palm chaff makes red soil
8 parts, 6 parts of sawdust, 6 parts of vermiculite, 6 parts of fertile soil.
The matrix of the second hardening bed described in step (3) includes the ingredient of following parts by weight：15 parts of field original soil, coconut palm chaff
6 parts, 3 parts of the dregs of a decoction；The dregs of a decoction are the extraction dregs of a decoction of aloe, garlic, mulberries and giant knotweed.
Step further includes the prevention and control of plant diseases, pest control in (3)：Outside bottle during hardening, every 20 days, alternating sprayed carbendazim
Or the solution of 0.1% concentration of thiophanate methyl 1 time.
Step further includes top dressing in (3)：During second of thermophilic hardening and room temperature hardening, every 30 days, use
Potassium dihydrogen phosphate aqueous solution foliage-spray is primary.
Embodiment 5
A kind of acclimatization and transplants method of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling, includes the following steps：
1) in-bottle seeding：Select start hardening mid-September, first carry out disinfection to culturing room sterilization processing, then by pale reddish brown trident
The culture bottle of Herba Phaii tankervilliaes tissue culture rooted seedling gradually takes off lid in culturing room, takes off lid within 3 days and finishes, during which environmental condition during tissue culture with protecting
It holds consistent；Culture bottle is then transferred to the position of hardening outside bottle, keeps ventilation, controls more than 88% shading, while keeps temperature
It is 20 DEG C to spend, and air humidity is more than 88%, transition culture 6 days；The disinfection processing is uses in culturing room
1150 μ W/cm2 ultraviolet lights sterilize 100min；
2) cleaning and dipping：It will be taken out from culture bottle by the pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling of in-bottle seeding, be divided into list
Strain is placed in the culture dish of the room temperature clear water equipped with 1.5cm depths, is impregnated 2.5h, is then repeated to change water and soak 5 times, change for the last time
After water, 2 days growth-promoting roots are persistently impregnated, seedling is then impregnated into 15min using plant extraction liquid；The system of the plant extraction liquid
Then Preparation Method first adds in the hot ethanol reflux 18min extractions of 8 times of weight, then for aloe, garlic, mulberries, giant knotweed is taken to smash to pieces
The boiling that 10 times of weight are added in into extraction vessel is boiled to ethyl alcohol volatilization completely, and filtering obtains the dregs of a decoction and filtrate, filtrate is placed
To room temperature to obtain the final product；
3) the outer hardening of bottle：
A, first time thermophilic hardening：Bletilla tissue culture rooted seedling Jing Guo cleaning and dipping is colonized in the matrix of the first hardening bed,
The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of coconut palm chaff again, and cladding thickness 1.5cm keeps logical
Wind controls more than 80% shading, while it is 20 DEG C to keep temperature, and air humidity 83%, water content of substrate keeps more than 78%,
Hardening 18 days；
B, low temperature hardening：Ventilation is kept, controls more than 75% shading, while it is 19 DEG C to keep temperature, air humidity 82%, base
Matter water content keeps more than 65%, carries out first low temperature hardening 12 days；Ventilation is kept, more than 75% shading is controlled, keeps simultaneously
Temperature is 15 DEG C, and air humidity 81%, water content of substrate keeps more than 65%, carries out low temperature hardening 13 days again；
C, second of thermophilic hardening：Bletilla tissue culture Jing Guo low temperature hardening is taken root into transplantation of seedlings in the matrix of the second hardening bed,
The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of crop field original soil again, and cladding thickness is 2~4cm,
Ventilation is kept, controls more than 70% shading, while it is 22 DEG C to keep temperature, air humidity 75%, water content of substrate gradually drops
To more than 64%, hardening 26 days；
D, room temperature hardening：Ventilation is kept, controls more than 70% shading, while temperature is controlled to be consistent with crop field temperature, air
More than 70% humidity, more than 60% water content of substrate, hardening 25 days, you can into field planting.
The matrix of the first hardening bed described in step (3) includes the ingredient of following parts by weight：20 parts of coconut palm chaff makes red soil
12 parts, 10 parts of sawdust, 10 parts of vermiculite, 10 parts of fertile soil.
The matrix of the second hardening bed described in step (3) includes the ingredient of following parts by weight：20 parts of field original soil, coconut palm chaff
10 parts, 5 parts of the dregs of a decoction；The dregs of a decoction are the extraction dregs of a decoction of aloe, garlic, mulberries and giant knotweed.
Embodiment 6
A kind of acclimatization and transplants method of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling, includes the following steps：
1) in-bottle seeding：Selection late October starts hardening, and first carry out disinfection to culturing room sterilization processing, then by pale reddish brown trident
The culture bottle of Herba Phaii tankervilliaes tissue culture rooted seedling gradually takes off lid in culturing room, takes off lid within 3 days and finishes, during which environmental condition during tissue culture with protecting
It holds consistent；Culture bottle is then transferred to the position of hardening outside bottle, keeps ventilation, controls more than 85% shading, while keeps temperature
It is 22 DEG C to spend, and air humidity is more than 89%, transition culture 5 days；The disinfection processing is uses in culturing room
1000 μ W/cm2 ultraviolet lights sterilize 110min；
2) cleaning and dipping：It will be taken out from culture bottle by the pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling of in-bottle seeding, be divided into list
Strain is placed in the culture dish of the room temperature clear water equipped with 1.6cm depths, is impregnated 2h, is then repeated to change water and soak 4 times, change water for the last time
Afterwards, 1 day growth-promoting root is persistently impregnated, seedling is then impregnated into 18min using plant extraction liquid；The preparation of the plant extraction liquid
Then method first adds in the hot ethanol reflux 28min extractions of 6 times of weight for aloe, garlic, mulberries, giant knotweed is taken to smash to pieces, then past
The boiling that 11 times of weight are added in extraction vessel is boiled complete to ethyl alcohol volatilization, and filtering obtains the dregs of a decoction and filtrate, by filtrate place to
Room temperature to obtain the final product；
3) the outer hardening of bottle：
A, first time thermophilic hardening：Bletilla tissue culture rooted seedling Jing Guo cleaning and dipping is colonized in the matrix of the first hardening bed,
The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of coconut palm chaff again, and cladding thickness 1cm keeps logical
Wind controls more than 80% shading, while it is 20 DEG C to keep temperature, and air humidity 80%, water content of substrate keeps more than 70%,
Hardening 22 days；
B, low temperature hardening：Ventilation is kept, controls more than 75% shading, while it is 18 DEG C to keep temperature, air humidity 80%, base
Matter water content keeps more than 65%, carries out first low temperature hardening 13 days；Ventilation is kept, more than 75% shading is controlled, keeps simultaneously
Temperature is 15 DEG C, and air humidity 80%, water content of substrate keeps more than 65%, carries out low temperature hardening 13 days again；
C, second of thermophilic hardening：Bletilla tissue culture Jing Guo low temperature hardening is taken root into transplantation of seedlings in the matrix of the second hardening bed,
The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of crop field original soil again, and cladding thickness 3cm is kept
Ventilation controls more than 70% shading, while it is 20 DEG C to keep temperature, and air humidity 76%, water content of substrate is gradually down to
More than 60%, hardening 22 days；
D, room temperature hardening：Ventilation is kept, controls more than 70% shading, while temperature is controlled to be consistent with crop field temperature, air
More than 70% humidity, more than 60% water content of substrate, hardening 28 days, you can into field planting.
The matrix of the first hardening bed includes the ingredient of following parts by weight：18 parts of coconut palm chaff makes 10 parts of red soil, sawdust
8 parts, 8 parts of vermiculite, 8 parts of fertile soil.
The matrix of the second hardening bed includes the ingredient of following parts by weight：18 parts of field original soil, 8 parts of coconut palm chaff, the dregs of a decoction 4
Part；The dregs of a decoction are the extraction dregs of a decoction of aloe, garlic, mulberries and giant knotweed.
Claims (10)
- A kind of 1. acclimatization and transplants method of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling, it is characterised in that include the following steps：1) in-bottle seeding：Select start hardening to March September to October or 2 months, first carry out disinfection to culturing room sterilization processing, so The culture bottle of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling in culturing room is gradually taken off into lid afterwards, lid is taken off within 2~3 days and finishes, during which ring Border condition during tissue culture with being consistent；Culture bottle is then transferred to the position of hardening outside bottle, keeps ventilation, controls shading 85% More than, while it is 20~22 DEG C to keep temperature, air humidity is more than 85%, transition culture 5~7 days；2) cleaning and dipping：It will be taken out from culture bottle by the pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling of in-bottle seeding, be divided into list Strain is placed in the culture dish of the room temperature clear water equipped with 0.5~2cm depth, 1~3h of immersion, then repeats to change water and soak 3~6 times, most After once changing water afterwards, 1~2 day growth-promoting root is persistently impregnated, seedling is then impregnated 10 using 800~1000 times of carbendazols~ 20min；3) the outer hardening of bottle：A, first time thermophilic hardening：Bletilla tissue culture rooted seedling Jing Guo cleaning and dipping is colonized in the matrix of the first hardening bed, The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of coconut palm chaff again, and cladding thickness is 1~3cm, is kept Ventilation controls more than 80% shading, while it is 20~22 DEG C to keep temperature, and air humidity is 80~85%, and water content of substrate is protected More than 70% is held, hardening 15~30 days；B, low temperature hardening：Ventilation is kept, controls more than 75% shading, while it is 17~19 DEG C to keep temperature, air humidity is 80%~82%, water content of substrate keeps more than 65%, carries out first low temperature hardening 10~15 days；Ventilation is kept, controls shading More than 75%, while it is 14~16 DEG C to keep temperature, air humidity is 80%~82%, and water content of substrate keeps more than 65%, Carry out low temperature hardening 10~15 days again；C, second of thermophilic hardening：Bletilla tissue culture Jing Guo low temperature hardening is taken root into transplantation of seedlings in the matrix of the second hardening bed, The depth of planting irrigates root water using not crab rooting as degree after cultivation, then cover one layer of crop field original soil again, and cladding thickness is 2~4cm, Ventilation is kept, controls more than 70% shading, while it is 20~22 DEG C to keep temperature, air humidity is 70~80%, and matrix is aqueous Amount is gradually down to more than 60%, hardening 15~30 days；D, room temperature hardening：Ventilation is kept, controls more than 70% shading, while temperature is controlled to be consistent with crop field temperature, air More than 70% humidity, more than 60% water content of substrate, hardening 20~30 days, you can into field planting.
- 2. the acclimatization and transplants method of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling according to claim 1, it is characterised in that step Suddenly the disinfection processing described in (1) is that 1000~1200 μ W/cm are used in culturing room2Ultraviolet light disinfection 60~ 120min。
- 3. the acclimatization and transplants method of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling according to claim 1, it is characterised in that step Suddenly 800~1000 times of carbendazols described in (2) can be substituted using plant extraction liquid.
- 4. the acclimatization and transplants method of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling according to claim 3, it is characterised in that institute Then the preparation method for the plant extraction liquid stated first adds in the heat of 6~8 times of weight for aloe, garlic, mulberries, giant knotweed is taken to smash to pieces 15~30min of alcohol reflux is extracted, and the boiling that 8~12 times of weight are then added in into extraction vessel is boiled to ethyl alcohol volatilization completely, Filtering, obtains the dregs of a decoction and filtrate, filtrate is placed to room temperature to obtain the final product.
- 5. the acclimatization and transplants method of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling according to claim 1, it is characterised in that step Suddenly the matrix described in (3) is to make red soil or containing the mixed-matrix for making red soil.
- 6. the acclimatization and transplants method of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling according to claim 5, it is characterised in that institute The red soil made red soil and refer to be less than without clay particle or clay particle content 1% stated.
- 7. the acclimatization and transplants method of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling according to claim 1, it is characterised in that step Suddenly the matrix of the first hardening bed described in (3) includes the ingredient of following parts by weight：15~20 parts of coconut palm chaff makes red soil 8~12 Part, 6~10 parts of sawdust, 6~10 parts of vermiculite, 6~10 parts of fertile soil.
- 8. the acclimatization and transplants method of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling according to claim 1, it is characterised in that step Suddenly the matrix of the second hardening bed described in (3) includes the ingredient of following parts by weight：15~20 parts of field original soil, coconut palm chaff 6~10 Part, 3~5 parts of the dregs of a decoction；The dregs of a decoction are the extraction dregs of a decoction of aloe, garlic, mulberries and giant knotweed.
- 9. the acclimatization and transplants method of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling according to claim 1, it is characterised in that step Suddenly the prevention and control of plant diseases, pest control is further included in (3)：Outside bottle during hardening, every 10~20 days, alternating sprayed carbendazim or methyl The solution of 0.1% concentration of thiophanate 1 time.
- 10. the acclimatization and transplants method of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling according to claim 1, it is characterised in that step Suddenly top dressing is further included in (3)：During second of thermophilic hardening and room temperature hardening, every 20~30 days, di(2-ethylhexyl)phosphate is used Hydrogen aqueous solutions of potassium foliage-spray is primary.
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| CN115191310A (en) * | 2022-07-14 | 2022-10-18 | 西藏自治区高原生物研究所 | Seedling group hardening method for orchids |
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