CN103314857A - Domestication cultivation method of dendrobium candidum tissue culture seedling - Google Patents
Domestication cultivation method of dendrobium candidum tissue culture seedling Download PDFInfo
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- CN103314857A CN103314857A CN 201310282955 CN201310282955A CN103314857A CN 103314857 A CN103314857 A CN 103314857A CN 201310282955 CN201310282955 CN 201310282955 CN 201310282955 A CN201310282955 A CN 201310282955A CN 103314857 A CN103314857 A CN 103314857A
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Abstract
The invention relates to a domestication cultivation method of a dendrobium candidum tissue culture seedling and belongs to the technical field of planting of traditional Chinese medicinal materials. The domestication cultivation method of the dendrobium candidum tissue culture seedling comprises the following steps of: preparing a matrix, exercising seedling, taking seedlings out bottles, carrying out domestication cultivation, and carrying out domestication management. The domestication cultivation method of the dendrobium candidum tissue culture seedling has the beneficial effects that an appropriate transition method from experiment bottles to nature is provided for the dendrobium candidum tissue culture seedling, the tissue culture seedling can rapidly improve an absorption function of root hairs, a dredging transportation function of stems and a photosynthesis function of blades and can adapt to the natural environment more rapidly, the transplanting survival rate of the tissue culture seedling can be improved, and domestication seedling transplanting survival rate can be more than 95% by applying the domestication cultivation method of the dendrobium candidum tissue culture seedling.
Description
Technical field
The invention belongs to the domesticating cultivation method in traditional Chinese medicine planting technology field, particularly a kind of candidum tissue culturing seedling.
Background technology
The stem of noble dendrobium is China's tradition rare traditional Chinese medicine, and it is all on the books that edition pharmacopeia is gone through by China.The former plant of the Chinese medicine stem of noble dendrobium be the orchid family (Orchidaceae) Dendrobium (
DendrobiumSw.) herbaceos perennial.Dendrobium is one of larger genus of the orchid family, and the whole world approximately has more than 1000 to plant, and China's Dendrobium Sw has 74 kind of 2 mutation, and wherein accounting for of circulation of commodities more than 50 planted.Wherein precious with dendrobium candidum, be the superfine product of all stems of noble dendrobium.Dendrobium candidum is endangered species, belongs to national secondary and lays special stress on protecting plant, and be famous and precious rare traditional Chinese medicine.Ancient times, the noble claimed that dendrobium candidum is " purple principal columns of a hall celestial being is pretty ", " Qian Nianrun ", claim again " the celestial grass of help " among the people.Authentic dendrobium candidum is the iron green, and gas is little, and that chews has a stickiness, distinguish the flavor of sweet, and few slag eats without any side effects for a long time.Be twisted into helical form and the rear title of oven dry while frying with dendrobium candidum
Tiepi Fengdou.
For many years, the commodity stem of noble dendrobium mainly relies on the Wild Medicinal Dendrobium Plants, and along with deepening continuously and the year by year increase of demand both at home and abroad of medicinal dendrobium development and use, China's Wild Medicinal Dendrobium Plants has suffered heavy damage, some area even face exhaustion.In order to protect Wild Orchids Resources; each state has all put into effect many statutes and regulations of forbidding excavating or selling Wild Orchids; in " the Conservation of resource management rules " of State Council's appearance in 1987, dendrobium loddigesii Rolfe, HERBA DENDROBII, HERBA DENDROBII, stem of Eyeshaped Dendrobium and dendrobium candidum are listed in three grades of Precious, Rare, Endangered protective plants of country.People begin gradually wild plant to dendrobium candidum and carry out house and plant cultivation, high-yield culture technique etc. and put into practice thus.The propagation technique of dendrobium candidum is mainly seminal propagation, division propagation and cottage propagation dual mode at present.
Collect because Seeds of Dendrobium Candidum is difficult, percentage of seedgermination is low, and seminal propagation, and seedling raise period is long, has greatly increased the cultivation cost of dendrobium candidum, is unfavorable for the artificial establishing in large scale of dendrobium candidum.So in current artificial cultivation, breeding is main to rely on division propagation and cottage propagation, but the method reproduction coefficient is low, both uneconomical, limited again the yield potentiality of dendrobium candidum, inconvenient Planting management is promoted, thereby the expansion cultivated area is suppressed.People have begun artificial propagation, and many places have adopted tissue culture propagation method with a large amount of seedling of quick acquisition; Because tissue culture propagation carries out in artificial environment, its oneself supports poor ability, particularly the photosynthesis function of dredging transportation function, blade of the absorption function of its coring, cane can not be gathered the right extraneous environment that adapts to, can't bring into play its due function fully, cause like this transplanting survival rate very low, therefore need to tame and to transplant smoothly, to improve its survival rate.
Summary of the invention
Technical problem to be solved by this invention is: a kind of domesticating method that increases candidum tissue culturing seedling transplanting success rate, better reform of nature circle environment makes the institutional framework of plant more substantial, with the success of assurance group training seedling replanting.
For solving the problems of the technologies described above, the technical solution adopted in the present invention is a kind of domesticating cultivation method of candidum tissue culturing seedling, comprises substrate preparation, and hardening, bottle outlet, domesticating and cultivating, domestication management five large steps is characterized in that concrete steps are as follows:
⑴ substrate preparation: with wood fragments bits straight the solarization until water content 4%-5% under the sun, then soak to do in 12 hours with 800 times in 1000 times+dichlorvos of thiophanate methyl and train in groups seedling to tame matrix for subsequent use.
⑵ hardening: will organize the training seedling and move to the hardening room together with the seedling bottle and carry out hardening, accept natural daylight by sunshade net, the first week, the intensity of illumination with natural daylight was controlled between the 2000lx-3000lx, second week is controlled at the intensity of illumination of natural daylight between the 3000lx-4000lx, the 3rd week, the intensity of illumination with natural daylight was controlled between the 4000lx-5000lx, temperature in the hardening room is controlled between 18 ℃-28 ℃, and relative air humidity is controlled between the 50%-60%, hardening 20-22 days.
⑶ after bottle outlet: hardening 20-22 days, bottle cap is opened, allowed group training seedling be in direct contact with outdoor environment 2-3 days, then choose more than the long 3cm of seedling, more than the thick 2mm of stem, more than stipes 3 joints, it is green that 4 above mounted blades of blade are, coring is more than 3, the group training seedling that the long 3cm of root is above injects a small amount of running water in the seedling bottle, take out gently together with medium with tweezers, put into the clear water basin and clean medium, then wash gently with running water.
⑷ domesticating and cultivating: the group training transplantation of seedlings that will rinse well to the special use in the special-purpose domestication of the candidum tissue culturing seedling canopy is tamed on the implant bed, at first in the thick group training seedling domestication matrix of implant bed tiling 4-6cm, and spray 3 ‰ liquor potassic permanganate and carry out disinfection, then will organize the training transplantation of seedlings on group training seedling domestication matrix, every pocket 2-3 strain, the distance between rows and hills of every pocket is 7cm * 9cm, dig the alveole of 2-3cm with finger, to organize the training shoot root must put into and build with matrix afterwards, the coring of 2-3mm length is exposed to outside the matrix, without compacting.
⑸ tame management: will tame the interior temperature of canopy and be controlled between 16 ℃-30 ℃, spray 4-6 every day, each 3-5min, and the first week was controlled at water content of substrate in the scope of 65%-70%, air humidity is controlled in the 88%-92% scope, and illuminance is controlled in the scope of 5000lx-6000lx; Later water content of substrate is controlled in the scope of 60%-65%, air humidity is controlled in the 70%-80% scope, and illuminance is controlled in the scope of 6000lx-8000lx; Transplant and sprayed 1 ‰ kalium nitrate fertilizers in rear 10 days, spray one time 5 ‰ Nitrogen, Phosphorus and Potassium every two weeks later; Tame after 45-55 days, newly grown 3-4 sheet young leaves, coring reaches more than 5, the domestication seedling of ground plant height more than 5cm.
The N-P-K content of described Nitrogen, Phosphorus and Potassium is N:P:K=10:10:10.
The special-purpose domestication of the described candidum tissue culturing seedling canopy defending party to the application is applied for a patent, and number of patent application is: 2013203813828.
The special-purpose domestication of the described candidum tissue culturing seedling implant bed defending party to the application is applied for a patent, and number of patent application is: 2013203813832.
The invention has the beneficial effects as follows as candidum tissue culturing seedling a suitable transition method from the test bottle to the Nature is provided, make the very enough absorption function of its coring, the photosynthesis functions of dredging transportation function, blade of cane improved very soon of group training seedling, reform of nature environment quickly, the transplanting survival rate of raising group training seedling, use the method, domestication transplantation of seedlings survival rate is reached more than 95%.
Embodiment
The invention will be further described below in conjunction with embodiment, and following examples are intended to illustrate the present invention rather than limitation of the invention further, should not limit protection scope of the present invention with this.
Embodiment 1.
With wood fragments bits straight solarization until then water content 4% is soaked with 800 times in 1000 times+dichlorvos of thiophanate methyl and made domestication seedling group training matrix in 12 hours under the sun.
On March 20th, 2012, Testing Team of our company will organize the training seedling and move to the hardening room together with 100 bottles in seedling bottle and carry out hardening, accept natural daylight by sunshade net, the first week, the intensity of illumination with natural daylight was controlled between the 2000lx-3000lx, second week is controlled at the intensity of illumination of natural daylight between the 3000lx-4000lx, the 3rd week, the intensity of illumination with natural daylight was controlled between the 4000lx-5000lx, and temperature in the hardening room is controlled between 18 ℃-28 ℃, and relative air humidity is controlled between the 50%-60%; April 10, bottle cap is opened, allow group training seedling be in direct contact with outdoor environment 2 days, April 12, choose more than the long 3cm of seedling, more than the thick 2mm of stem, more than stipes 3 joints, it is green that 4 above mounted blades of blade are, and coring is more than 3, the above group training seedling of the long 3cm of root is tamed, at first in the seedling bottle, inject a small amount of running water, take out gently together with medium with tweezers, put into the clear water basin and clean medium, then wash gently with running water, wash out 4593 strains of qualified clusters training seedling; Then the group training transplantation of seedlings that will rinse well to the special use in the special-purpose domestication of the candidum tissue culturing seedling canopy is tamed on the implant bed, the long 500cm of this implant bed, wide 120cm, at first in the thick domestication seedling group training matrix of kind of planting-line tiling 4-6cm, and spray 3 ‰ liquor potassic permanganate and carry out disinfection, then will organize the training transplantation of seedlings on matrix, every pocket 2-3 strain, the distance between rows and hills of every pocket is 7cm * 9cm, dig the alveole of 2-3cm with finger, to organize the training shoot root must put into and build with matrix afterwards, the coring of 2-3mm length is exposed to outside the matrix, without compacting, cultivates altogether two furrow, 1760 pockets; Then will tame the interior temperature of canopy is controlled between 16 ℃-30 ℃, spray 4-6 every day, each 3-5min, and the first week was controlled at water content of substrate in the scope of 65%-70%, air humidity is controlled in the 88%-92% scope, and illuminance is controlled in the scope of 5000lx-6000lx; Later water content of substrate is controlled in the scope of 60%-65%, air humidity is controlled in the 70%-80% scope, and illuminance is controlled in the scope of 6000lx-8000lx; April 23 sprayed 1 ‰ kalium nitrate fertilizers, had then respectively sprayed one time 5 ‰ Nitrogen, Phosphorus and Potassium in May 8, May 23; May 26, to observe statistics and newly grown 3-4 sheet young leaves, coring reaches more than 5, qualified domestication seedling 4377 strains of ground plant height more than 5cm, survival rate reaches 95.3%.
Embodiment 2.
With wood fragments bits straight solarization until then water content 4.5% is soaked with 800 times in 1000 times+dichlorvos of thiophanate methyl and made domestication seedling group training matrix in 12 hours under the sun.
On April 10th, 2012, Testing Team of our company will organize the training seedling and move to the hardening room together with 150 bottles in seedling bottle and carry out hardening, accept natural daylight by sunshade net, the first week, the intensity of illumination with natural daylight was controlled between the 2000lx-3000lx, second week is controlled at the intensity of illumination of natural daylight between the 3000lx-4000lx, the 3rd week, the intensity of illumination with natural daylight was controlled between the 4000lx-5000lx, and temperature in the hardening room is controlled between 18 ℃-28 ℃, and relative air humidity is controlled between the 50%-60%; May 1, bottle cap is opened, allow group training seedling be in direct contact with outdoor environment 3 days, then choose more than the long 3cm of seedling, more than the thick 2mm of stem, more than stipes 3 joints, it is green that 4 above mounted blades of blade are, coring is more than 3, and the above group training seedling of the long 3cm of root is tamed, and at first injects a small amount of running water in the seedling bottle, take out gently together with medium with tweezers, put into the clear water basin and clean medium, then wash gently with running water, wash out 6899 strains of qualified clusters training seedling; Then the long 500cm of this implant bed, wide 120cm are tamed on the implant bed in the group training transplantation of seedlings that will rinse well to the special use in the special-purpose domestication of the candidum tissue culturing seedling canopy; Group training transplantation of seedlings to the special use domestication in the special-purpose domestication of the candidum tissue culturing seedling canopy of rinsing well is planted on the planting-line, at first in kind of the thick matrix of planting-line tiling 4-6cm, and spray 3 ‰ liquor potassic permanganate and carry out disinfection, then will organize the training transplantation of seedlings on matrix, every pocket 2-3 strain, the distance between rows and hills of every pocket is 7cm * 9cm, dig the alveole of 2-3cm with finger, to organize the training shoot root must put into and build with matrix afterwards, the coring of 2-3mm length is exposed, without compacting, three domestications kind of planting-line 2640 pockets have been cultivated altogether; Then will tame the interior temperature of canopy is controlled between 16 ℃-30 ℃, spray 4-6 every day, each 3-5min, and the first week was controlled at water content of substrate in the scope of 65%-70%, air humidity is controlled in the 88%-92% scope, and illuminance is controlled in the scope of 5000lx-6000lx; Later water content of substrate is controlled in the scope of 60%-65%, air humidity is controlled in the 70%-80% scope, and illuminance is controlled in the scope of 6000lx-8000lx; May 15 sprayed 1 ‰ kalium nitrate fertilizers, had respectively sprayed one time 5 ‰ Nitrogen, Phosphorus and Potassium on May 30, June 14; June 20, statistics was newly grown 3-4 sheet young leaves, and coring reaches more than 5, domestication seedling 6669 strains of ground plant height more than 5cm, and survival rate reaches 96.67%.
Embodiment 3.
With wood fragments bits straight solarization until then water content 5% is soaked with 800 times in 1000 times+dichlorvos of thiophanate methyl and made domestication seedling group training matrix in 12 hours under the sun.
On April 20th, 2012, Testing Team of our company will organize the training seedling and move to the hardening room together with 100 bottles in seedling bottle and carry out hardening, accept natural daylight by sunshade net, the first week, the intensity of illumination with natural daylight was controlled between the 2000lx-3000lx, second week is controlled at the intensity of illumination of natural daylight between the 3000lx-4000lx, the 3rd week, the intensity of illumination with natural daylight was controlled between the 4000lx-5000lx, and temperature in the hardening room is controlled between 18 ℃-28 ℃, and relative air humidity is controlled between the 50%-60%; May 12, bottle cap is opened, allow group training seedling be in direct contact with outdoor environment 3 days, then choose more than the long 3cm of seedling, more than the thick 2mm of stem, more than stipes 3 joints, it is green that 4 above mounted blades of blade are, coring is more than 3, and the group training seedling that the long 3cm of root is above injects a small amount of running water in the seedling bottle, take out gently together with medium with tweezers, put into the clear water basin and clean medium, then wash gently with running water, wash out 4581 strains of qualified clusters training seedling; Then the group training transplantation of seedlings that will rinse well to the special use in the special-purpose domestication of the candidum tissue culturing seedling canopy is tamed on the implant bed, the long 500cm of this implant bed, wide 120cm, at first in kind of the thick matrix of planting-line tiling 4-6cm, and spray 3 ‰ liquor potassic permanganate and carry out disinfection, then will organize the training transplantation of seedlings on matrix, every pocket 2-3 strain, the distance between rows and hills of every pocket is 7cm * 9cm, dig the alveole of 2-3cm with finger, to organize the training shoot root must put into and build with matrix afterwards, the coring of 2-3mm length is exposed, without compacting; Then will tame the interior temperature of canopy is controlled between 16 ℃-30 ℃, spray 4-6 every day, each 3-5min, and the first week was controlled at water content of substrate in the scope of 65%-70%, air humidity is controlled in the 88%-92% scope, and illuminance is controlled in the scope of 5000lx-6000lx; Later water content of substrate is controlled in the scope of 60%-65%, air humidity is controlled in the 70%-80% scope, and illuminance is controlled in the scope of 6000lx-8000lx; Spray 1 ‰ kalium nitrate fertilizers on May 25, have respectively sprayed one time 5 ‰ Nitrogen, Phosphorus and Potassium on June 9, June 23; June 26, statistics is newly grown 3-4 sheet young leaves, and coring reaches more than 5, qualified domestication seedling 4369 strains of ground plant height more than 5cm, and survival rate reaches 95.37%.
Claims (1)
1. the domesticating cultivation method of candidum tissue culturing seedling comprises substrate preparation, and hardening, bottle outlet, domesticating and cultivating, domestication management five large steps is characterized in that concrete steps are as follows:
⑴ substrate preparation: with wood fragments bits straight the solarization until water content 4%-5% under the sun, then soak to do in 12 hours with 800 times in 1000 times+dichlorvos of thiophanate methyl and train in groups seedling to tame matrix for subsequent use;
⑵ hardening: will organize the training seedling and move to the hardening room together with the seedling bottle and carry out hardening, accept natural daylight by sunshade net, the first week, the intensity of illumination with natural daylight was controlled between the 2000lx-3000lx, second week is controlled at the intensity of illumination of natural daylight between the 3000lx-4000lx, the 3rd week, the intensity of illumination with natural daylight was controlled between the 4000lx-5000lx, temperature in the hardening room is controlled between 18 ℃-28 ℃, and relative air humidity is controlled between the 50%-60%, hardening 20-22 days;
⑶ after bottle outlet: hardening 20-22 days, bottle cap is opened, allowed group training seedling be in direct contact with outdoor environment 2-3 days, then choose more than the long 3cm of seedling, more than the thick 2mm of stem, more than stipes 3 joints, it is green that 4 above mounted blades of blade are, coring is more than 3, the group training seedling that the long 3cm of root is above injects a small amount of running water in the seedling bottle, take out gently together with medium with tweezers, put into the clear water basin and clean medium, then wash gently with running water;
⑷ domesticating and cultivating: the group training transplantation of seedlings that will rinse well to the special use in the special-purpose domestication of the candidum tissue culturing seedling canopy is tamed on the implant bed, at first in the thick group training seedling domestication matrix of implant bed tiling 4-6cm, and spray 3 ‰ liquor potassic permanganate and carry out disinfection, then will organize the training transplantation of seedlings on group training seedling domestication matrix, every pocket 2-3 strain, the distance between rows and hills of every pocket is 7cm * 9cm, dig the alveole of 2-3cm with finger, to organize the training shoot root must put into and build with matrix afterwards, the coring of 2-3mm length is exposed to outside the matrix, without compacting;
⑸ tame management: will tame the interior temperature of canopy and be controlled between 16 ℃-30 ℃, spray 4-6 every day, each 3-5min, and the first week was controlled at water content of substrate in the scope of 65%-70%, air humidity is controlled in the 88%-92% scope, and illuminance is controlled in the scope of 5000lx-6000lx; Later water content of substrate is controlled in the scope of 60%-65%, air humidity is controlled in the 70%-80% scope, and illuminance is controlled in the scope of 6000lx-8000lx; Transplant and sprayed 1 ‰ kalium nitrate fertilizers in rear 10 days, spray one time 5 ‰ Nitrogen, Phosphorus and Potassium every two weeks later; Tame after 45-55 days, newly grown 3-4 sheet young leaves, coring reaches more than 5, the domestication seedling of ground plant height more than 5cm;
The N-P-K content of described Nitrogen, Phosphorus and Potassium is N:P:K=10:10:10.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103918558A (en) * | 2014-05-04 | 2014-07-16 | 中国热带农业科学院橡胶研究所 | Method for domesticating dendrobium officinale kimuraet migo tissue culture seedling by using glass bottle |
| CN106718712A (en) * | 2016-11-10 | 2017-05-31 | 四川省农业科学院经济作物育种栽培研究所 | A kind of method that candidum tissue culturing seedling transplants domestication |
| CN106937593A (en) * | 2017-02-05 | 2017-07-11 | 安徽中升生物科技有限公司 | A kind of tissue culture method of the stem of noble dendrobium |
| CN107027606A (en) * | 2017-05-17 | 2017-08-11 | 贵州济生农业科技有限公司 | A kind of cultural method of the high dendrobium candidum of stem of noble dendrobium alkali content |
| CN107548903A (en) * | 2017-08-22 | 2018-01-09 | 苏州寿丰农业科技有限公司 | A kind of seedling cultivation method of dendrobium candidum |
| CN110771489A (en) * | 2019-11-26 | 2020-02-11 | 浙江省亚热带作物研究所 | Dendrobium officinale cultivation device and cultivation method |
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2013
- 2013-07-08 CN CN 201310282955 patent/CN103314857A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103918558A (en) * | 2014-05-04 | 2014-07-16 | 中国热带农业科学院橡胶研究所 | Method for domesticating dendrobium officinale kimuraet migo tissue culture seedling by using glass bottle |
| CN103918558B (en) * | 2014-05-04 | 2016-03-23 | 中国热带农业科学院橡胶研究所 | A kind of method of candidum tissue culturing seedling glass bottle domestication |
| CN106718712A (en) * | 2016-11-10 | 2017-05-31 | 四川省农业科学院经济作物育种栽培研究所 | A kind of method that candidum tissue culturing seedling transplants domestication |
| CN106718712B (en) * | 2016-11-10 | 2020-11-24 | 四川省农业科学院经济作物育种栽培研究所 | Method for transplanting and domesticating dendrobium officinale tissue culture seedlings |
| CN106937593A (en) * | 2017-02-05 | 2017-07-11 | 安徽中升生物科技有限公司 | A kind of tissue culture method of the stem of noble dendrobium |
| CN107027606A (en) * | 2017-05-17 | 2017-08-11 | 贵州济生农业科技有限公司 | A kind of cultural method of the high dendrobium candidum of stem of noble dendrobium alkali content |
| CN107548903A (en) * | 2017-08-22 | 2018-01-09 | 苏州寿丰农业科技有限公司 | A kind of seedling cultivation method of dendrobium candidum |
| CN110771489A (en) * | 2019-11-26 | 2020-02-11 | 浙江省亚热带作物研究所 | Dendrobium officinale cultivation device and cultivation method |
| CN110771489B (en) * | 2019-11-26 | 2022-03-25 | 浙江省亚热带作物研究所 | Dendrobium officinale cultivation device and cultivation method |
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Application publication date: 20130925 |