CN107027606A - A kind of cultural method of the high dendrobium candidum of stem of noble dendrobium alkali content - Google Patents
A kind of cultural method of the high dendrobium candidum of stem of noble dendrobium alkali content Download PDFInfo
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Abstract
The invention discloses a kind of cultural method of the high dendrobium candidum of stem of noble dendrobium alkali content, comprise the following steps：（1）Select seedling；（2）Seedling is handled；（3）Greenhouse is built；（4）Transplant；（5）Temperature and Humidity Control；（6）Rich water quality management；（7）The prevention and control of plant diseases, pest control；（8）Harvesting.During the inventive method culture dendrobium candidum, the dendrobium candidum quality better of final output, active constituent content is high, particularly the height of the content of dendrobine.
Description
Technical field
The present invention relates to a kind of cultural method of dendrobium candidum, the cultivation of the high dendrobium candidum of particularly a kind of stem of noble dendrobium alkali content
Culture method.
Background technology
Dendrobium candidum nickname iron sheet orchid, bracketplant, Yunnan iron sheet, iron sheet bracketplant, are orchid family Dendrobium herbaceos perennials,
Dendrobium candidum is the top grade of Shihu " medicinal materials, have the laudatory title of " gold in Chinese celestial grass-medicine ", is used as medicine with stem, contains polysaccharide, stone
The active ingredients such as dry measure used in former times alkali, alkaloid, with nourishing Yin and clearing heat, the beneficial stomach that promotes the production of body fluid, wet one's whistle improving eyesight the effects such as, it is antitumor, improve human body
In terms of immunity, treatment enterogastric diseases, anti-aging, anti-oxidant, platelet aggregation-against, reduction blood glucose, treatment cataract
There is good efficacy.
Modern pharmacology research shows that dendrobium candidum has humidification to non-specific and specific immunity, anti-swollen
In terms of the treatment of knurl, anti-oxidant, reduction blood glucose and cataract, pharmacological action is notable.Its chemical composition is more complicated, it has been determined that
Type of compounds mainly have：Alkaloids, polysaccharide, luxuriant and rich with fragrance class, bibenzyl parent nucleus class, wherein alkaloids, polysaccharide pharmacology are lived
Property is more notable.Stem of noble dendrobium alkaline sesquiterpenoids alkaloid is that Dendrobium Sw is peculiar, and wherein dendrobine is earliest discovery, and research is most
Many alkaloids；HERBA DENDROBII alkali is similar to non-steroid anti-inflammatory drug pharmacological action, with immunological regulation, anti-inflammatory, expansion blood vessel
Deng effect.
As the health-care efficacy of dendrobium candidum is widely recognized and received by people, dendrobium officinale far can not expire
The demand of sufficient people.People have begun to use in artificial cultivation dendrobium candidum, the cultural method of dendrobium candidum, and culture medium is iron
Skin stem of noble dendrobium conditions on which persons or things depend for existence, different culture raw material and with can be to the growth of dendrobium candidum, yield and active component etc.
Produce important influence.The culture medium of dendrobium candidum and existing cultural method, are unfavorable for the iron sheet stone of output in the prior art
The quality guarantee of dry measure used in former times.Particularly it cannot be guaranteed that stem of noble dendrobium alkali content in dendrobium candidum so that the stem of noble dendrobium in the dendrobium candidum finally given
Alkali content is low.
The content of the invention
The purpose of the present invention, is to provide a kind of cultural method of the high dendrobium candidum of stem of noble dendrobium alkali content, the inventive method training
When supporting dendrobium candidum, the dendrobium candidum quality better of final output, active constituent content is high, particularly the height of the content of dendrobine.
What the present invention was realized in.
A kind of cultural method of the high dendrobium candidum of stem of noble dendrobium alkali content, comprises the following steps：
(1) seedling is selected：Selection fleshy stem has 3~4 internodes, with 4~5 leaves, and long more than the 3cm of root has 4-5 bar roots, root
With green, no black root during color of the leather is white, no deformity, the dendrobium candidum seedling of no variation；
(2) seedling is handled：Dendrobium candidum seedling root is carried out after cleaning 3-4 times, seedling root is placed in 1000mg/L
Heteroauxin in immersion 20-30min after, the dendrobium candidum seedling after must handling；
(3) greenhouse construction：Build wide 6-8 meters, long 28-32 meters, 110-120 meters of canopy shoulder height, the high 3-4min of ceiling plantation
Greenhouse, the shade net of the good 70-80% shade densities of ceiling plastic covering fogging-resistance film installs running water pipe in canopy, greenhouse surrounding and
Entrance is equipped with the fly net of 30-40 mesh；Overhead kind of furrow bottom planting-line is built in canopy, with framework of the yoke as kind of planting-line, then
The plastic board that laying aperture is 0.3-0.5cm is as matrix support face, and furrow bottom overhead height is 30-50cm, then in furrow face
Upper laying 8-12cm matrix；
(4) transplant：Transplanting time is March, and the deep duck eyes of 2-3cm are dug in the matrix of kind of planting-line, take dendrobium candidum to transplant
Seedling, is diluted after the sterilization of immersion root with 0.3-0.5% potassium permanganate, seedling root is put into duck eye, matrix lid is then used
Good, planting density is 100-200 clump/square metre, every clump 2-5 plants, and clump is away from for 5-10cm；
(5) Temperature and Humidity Control：Ensure that canopy temperature is maintained at 18-22 DEG C daytime, at 15-20 DEG C of night, humid control exists
80%-90%, 0.4-0.6% aqueous solution of urea is sprayed for 10-15 days after being transplanted after transplanting；
(6) rich water quality management：Control matrix normal moisture content in 30-40%, air humidity keeps 60%-70%, per 10-15
Its 1 water of spray, can spray 1 0.5%~0.8% potassium dihydrogen phosphate aqueous solution per 15-20 days；
(7) prevention and control of plant diseases, pest control：It is monthly water-soluble with 1000 times of 40% dimethoate emulsifiable concentrate, 1000 times of aqueous solution or 50% malathion
Liquid sprays carry out foliar control, and insect prevention uses fly net；
(8) harvest：Harvested between April November to next year, cut and stay small greatly, cut and stay tender always.
In the cultural method of the high dendrobium candidum of foregoing stem of noble dendrobium alkali content, the step (3), the matrix it is main by by
1-5 parts of 1-5 parts of 1-5 parts of 10-20 parts of mashed potatoes, urea, phosphorus pentoxide, diammonium hydrogen phosphate, the sodium tetraborate of listed as parts by weight
1-3 parts, 10-20 parts of Chinese cassia tree, 10-20 parts of Morinda officinalis, 20-30 parts of Chinese lobelia, 15-25 parts of Jiang Xia, 15-25 parts of the coptis, tuber of dwarf lilyturf 10-
20 parts, 10-20 parts of the dried rhizome of rehmannia, 15-25 parts of Fructus Corni, 15-25 parts of the red sage root, 10-20 parts of American Ginseng, 20-30 parts of peach kernel, moutan bark
20-30 parts prepare as follows with 20-30 parts of the root of kudzu vine：Take Chinese cassia tree, Morinda officinalis, Chinese lobelia, Jiang Xia, the coptis, the tuber of dwarf lilyturf, the dried rhizome of rehmannia,
Fructus Corni, the red sage root, American Ginseng, peach kernel, moutan bark and the root of kudzu vine, plus the 8-12 times of decocting measured are boiled after 3-5h, filtering, take filter residue, are done
After dry, coarse granule is ground into, coarse granule is mixed with mashed potatoes, urea, phosphorus pentoxide, diammonium hydrogen phosphate and sodium tetraborate, i.e.,
.
The cultural method of the high dendrobium candidum of foregoing stem of noble dendrobium alkali content, the main soil by calculating by weight of the matrix
12-18 parts of beans mud, 2-4 parts of urea, 2-4 parts of phosphorus pentoxide, 2-4 parts of diammonium hydrogen phosphate, 2-3 parts of sodium tetraborate, Chinese cassia tree 12-18
Part, 12-18 parts of Morinda officinalis, 22-28 parts of Chinese lobelia, 17-23 parts of Jiang Xia, 17-23 parts of the coptis, tuber of dwarf lilyturf 12-18 part, dried rhizome of rehmannia 12-18
Part, 17-23 parts of Fructus Corni, 17-23 parts of the red sage root, 12-18 parts of American Ginseng, 23-27 parts of peach kernel, 23-27 parts of moutan bark and root of kudzu vine 23-
27 parts prepare as follows：Take Chinese cassia tree, Morinda officinalis, Chinese lobelia, Jiang Xia, the coptis, the tuber of dwarf lilyturf, the dried rhizome of rehmannia, Fructus Corni, the red sage root, the West
Ginseng, peach kernel, moutan bark and the root of kudzu vine, plus the 8-12 times of decocting measured are boiled after 3-5h, filtering, take filter residue, after drying, are ground into thick
Grain, coarse granule is mixed with mashed potatoes, urea, phosphorus pentoxide, diammonium hydrogen phosphate and sodium tetraborate, is produced.
The cultural method of the high dendrobium candidum of foregoing stem of noble dendrobium alkali content, the main soil by calculating by weight of the matrix
15 parts of beans mud, 3 parts of urea, 3 parts of phosphorus pentoxide, 3 parts of diammonium hydrogen phosphate, 2.5 parts of sodium tetraborate, 15 parts of Chinese cassia tree, Morinda officinalis 15
Part, 25 parts of Chinese lobelia, 20 parts of Jiang Xia, 20 parts of the coptis, 15 parts of the tuber of dwarf lilyturf, 15 parts of the dried rhizome of rehmannia, 20 parts of Fructus Corni, 20 parts of the red sage root, American Ginseng
25 parts of 15 parts, 25 parts of peach kernel, 25 parts of moutan bark and the root of kudzu vine are prepared as follows：Take Chinese cassia tree, Morinda officinalis, Chinese lobelia, Jiang Xia, Huang
Company, the tuber of dwarf lilyturf, the dried rhizome of rehmannia, Fructus Corni, the red sage root, American Ginseng, peach kernel, moutan bark and the root of kudzu vine, plus the 8-12 times of decocting measured are boiled after 3-5h, mistake
Filter, takes filter residue, after drying, and is ground into coarse granule, coarse granule and mashed potatoes, urea, phosphorus pentoxide, diammonium hydrogen phosphate and four boron
Sour sodium is mixed, and is produced.
Applicant has carried out substantial amounts of experimental study to the present invention, and part Experiment is as follows：
The stem of noble dendrobium alkali content of experimental example 1 investigates
1 materials and methods
1.1 material
1.1.1 dendrobium candidum 1：Cultivated by embodiment 1, harvesting obtains dendrobium candidum 1.
1.1.2 dendrobium candidum 2：During cultivation, Chinese cassia tree, Morinda officinalis, Chinese lobelia, Jiang Xia, the coptis, the tuber of dwarf lilyturf, life are free of in matrix
Ground, Fructus Corni, the red sage root, American Ginseng, peach kernel, moutan bark and the root of kudzu vine, after remaining method be the same as Example 1 is cultivated, harvesting is obtained
Dendrobium candidum 2.
1.2 instruments and reagent
Dendrobine standard items：Chengdu Man Site Bioisystech Co., Ltd, MUST-12080608.AE240 electronic balances：
Switzerland MEITLER；HH-s digital control constant temperature water-baths：Jinhua Wenhua science and education laboratory apparatus factory；The Rotary Evaporators of BM 510：Japan
YAMATO companies；6890N gas chromatographs：Agilent company of the U.S.；UV-2450 ultraviolet specrophotometers：Japanese Shimadzu Corporation；
Methanol (chromatographically pure)：Tianjin Kermel Chemical Reagent Co., Ltd.；Remaining reagent is that analysis is pure；Phenol：Insult peaking in Shanghai
Learn reagent Co., Ltd；Sulfuric acid：Shanghai Ling Feng chemical reagent Co., Ltd.
2 methods
The preparation of 2.1 need testing solutions
2.1.1 the preparation of need testing solution 1：
Dendrobium candidum of the present invention (dendrobium candidum 1) sample is taken, 50 DEG C is put in baking oven, dry, pulverize, No. three sieves is crossed, took
Medicinal powder 3.34g after filter, plus the ethanol of 200mL 90%, flow back 1 hour, filtering, pour out supernatant, then add 100mL90% second
Alcohol, is flowed back 1 hour, pours out supernatant, merges above-mentioned 2 supernatants, puts Rotary Evaporators concentration, and 50 DEG C of water-bath is evaporated, and is done
Dry product add methanol to dissolve, and are settled to 25m L, produce need testing solution 1.
2.1.2 the preparation of need testing solution 2
The sample of dendrobium candidum 2 is taken, 50 DEG C is put in baking oven, dry, pulverize, No. three sieves is crossed, takes medicinal powder after filtering
3.34g, plus the ethanol of 200mL 90%, flow back 1 hour, filtering, pour out supernatant, then add 100mL90% ethanol, flow back 1 hour,
Supernatant is poured out, merges above-mentioned 2 supernatants, Rotary Evaporators concentration is put, 50 DEG C of water-bath is evaporated, and dry product adds methanol molten
Solution, is settled to 25m L, produces need testing solution 2.
The preparation of 2.2 reference substance solutions
Precision weighs dendrobine reference substance 50.01lmg into 100mL measuring bottles, plus methanol constant volume is to scale, shakes up, and obtains
500.1m g/L dendrobine reference substance mother liquors.50.01m g/L solution, it is standby as reference substance solution.
2.3 chromatographic condition
Chromatographic column：D B-1 capillary columns (30mm × 0.25mm, 0.25um)；Temperature programming：Initial temperature is 80 DEG C, with
10 DEG C/min speed is warming up to 250 DEG C, is kept for 5 minutes；Injector temperature is 250 DEG C, and detector temperature is 250 DEG C, sample introduction
10uL is measured, theoretical cam curve is not less than 10000 based on dendrobine.
It is prepared by 2.4 standard curves
Precision measure reference substance solution 0.2,0.5,0.8,1.0,2.0,4.0m L be respectively placed in 10m L measuring bottles, plus first
Alcohol shakes up to scale.Determined by above-mentioned chromatographic condition.Using integrating peak areas as ordinate, reference substance sample size enters for abscissa
Row linear regression, regression equation is A=0.7522C-0.0252, r=0.9997, shows dendrobine in 0.10-2.00ug scopes
It is interior that there is good linear relationship.
2.5 precision test
Precision draws dendrobine reference substance solution l0ul, and continuous 6 sample introductions record peak area, the RSD of dendrobine peak area
For 1.58%, show that instrument precision is good.
2.6 replica test
6 parts of need testing solutions 1 and need testing solution 2 are prepared as stated above, are measured by above-mentioned chromatographic condition.The stem of noble dendrobium
The RSD of alkali peak area is respectively 2.05% and 2.06%.
2.7 stability test
Take same need testing solution 1, place at room temperature, respectively at 0,2,4,6,8, l2 hours sample introductions, record dendrobine
Peak area, RSD is 2.88%.As a result show, need testing solution 1 is stable in 12 hours.
Take same need testing solution 2, place at room temperature, respectively at 0,2,4,6,8, l2 hours sample introductions, record dendrobine
Peak area, RSD is 2.89%.As a result show, need testing solution 2 is stable in 12 hours.
2.8 recovery test
Take known content 0.46mg/g) 6 parts of dendrobium candidum powder, every part of about 1.65g is accurately weighed.It is accurate to add stone
Dry measure used in former times alkali reference substance mother liquor 2mL, prepares need testing solution 1 and need testing solution 2 as stated above, is carried out under above-mentioned chromatographic condition
Determine, calculate the rate of recovery.As a result average recovery rate is 97.1% and 96.8%, RSD are respectively 2.5% and 2.6%.
2.9 sample sizes are determined
By the preparation method and condition determination of above-mentioned need testing solution 1 and need testing solution 2, it is divided into 6 parts, determines 6 parts of iron
The content of dendrobine in the sample of the skin stem of noble dendrobium 1 and 6 parts of samples of dendrobium candidum 2, records result, and results averaged the results are shown in Table 1.
The dendrobine assay result of table 1
As seen from table, dendrobine contains in the dendrobium candidum that the inventive method cultivation is obtained, the dendrobium candidum finally harvested
Amount is higher.The quality better of dendrobium candidum.
Compared with prior art, during the inventive method culture dendrobium candidum, the dendrobium candidum quality better of final output, effectively
Component content is high, particularly the height of the content of dendrobine.
Embodiment
Embodiment 1.
A kind of cultural method of the high dendrobium candidum of stem of noble dendrobium alkali content, comprises the following steps：
(1) seedling is selected：Selection fleshy stem has 3~4 internodes, with 4~5 leaves, and long more than the 3cm of root has 4-5 bar roots, root
With green, no black root during color of the leather is white, no deformity, the dendrobium candidum seedling of no variation；
(2) seedling is handled：Dendrobium candidum seedling root is carried out after cleaning 3 times, seedling root is placed in 1000mg/L's
Soaked in heteroauxin after 25min, the dendrobium candidum seedling after must handling；
(3) greenhouse construction：Build wide 6-8 meters, long 30 meters, 115 meters of canopy shoulder height, the high 3.5min of ceiling planting greenhouse, canopy
Push up and running water pipe is installed in the shade net of the shade density of plastic covering fogging-resistance film 75%, canopy, greenhouse surrounding and entrance are equipped with 40
Purpose fly net；Overhead kind of furrow bottom planting-line is built in canopy, with framework of the yoke as kind of planting-line, it is 0.3- then to lay aperture
0.5cm plastic board is as matrix support face, and furrow bottom overhead height is 30-50cm, then lays 8-12cm's in furrow face
Matrix；The matrix by mashed potatoes 15kg, urea 3kg, phosphorus pentoxide 3kg, diammonium hydrogen phosphate 3kg, sodium tetraborate 2.5kg,
Chinese cassia tree 15kg, Morinda officinalis 15kg, Chinese lobelia 25kg, Jiang Xia 20kg, coptis 20kg, tuber of dwarf lilyturf 15kg, dried rhizome of rehmannia 15kg, Fructus Corni
20kg, red sage root 20kg, American Ginseng 15kg, peach kernel 25kg, moutan bark 25kg and root of kudzu vine 25kg are prepared as follows：Take Chinese cassia tree,
Morinda officinalis, Chinese lobelia, Jiang Xia, the coptis, the tuber of dwarf lilyturf, the dried rhizome of rehmannia, Fructus Corni, the red sage root, American Ginseng, peach kernel, moutan bark and the root of kudzu vine, plus 10 times
The decocting of amount is boiled after 4h, filtering, takes filter residue, after drying, and is ground into coarse granule, coarse granule and mashed potatoes, urea, five oxidations two
Phosphorus, diammonium hydrogen phosphate and sodium tetraborate are mixed, and are produced；
(4) transplant：Transplanting time is March, and the deep duck eyes of 2-3cm are dug in the matrix of kind of planting-line, take dendrobium candidum to transplant
Seedling, is diluted after the sterilization of immersion root with 0.4% potassium permanganate, seedling root is put into duck eye, then covered with matrix, planted
Training density is 100-200 clumps/square metre, and every clump 2-5 plants, clump is away from for 5-10cm；
(5) Temperature and Humidity Control：Ensure that canopy temperature is maintained at 18-22 DEG C daytime, at 15-20 DEG C of night, humid control exists
80%-90%, 0.4-0.6% aqueous solution of urea is sprayed in 13 days after being transplanted after transplanting；
(6) rich water quality management：Control matrix normal moisture content in 30-40%, air humidity keeps 60%-70%, per 10-15
Its 1 water of spray, can spray 1 0.5%~0.8% potassium dihydrogen phosphate aqueous solution per 15-20 days；
(7) prevention and control of plant diseases, pest control：Monthly sprayed with 40% dimethoate emulsifiable concentrate, 1000 times of aqueous solution and carry out foliar control, insect prevention is used
Fly net；
(8) harvest：Harvested between April November to next year, cut and stay small greatly, cut and stay tender always.
Embodiment 2.
A kind of cultural method of the high dendrobium candidum of stem of noble dendrobium alkali content, comprises the following steps：
(1) seedling is selected：Selection fleshy stem has 3~4 internodes, with 4~5 leaves, and long more than the 3cm of root has 4-5 bar roots, root
With green, no black root during color of the leather is white, no deformity, the dendrobium candidum seedling of no variation；
(2) seedling is handled：Dendrobium candidum seedling root is carried out after cleaning 4 times, seedling root is placed in 1000mg/L's
Soaked in heteroauxin after 30min, the dendrobium candidum seedling after must handling；
(3) greenhouse construction：Wide 8 meters is built, long 32 meters, 120 meters of canopy shoulder height, the high 4min of ceiling planting greenhouse, ceiling covers
Running water pipe is installed, greenhouse surrounding and entrance are equipped with 40 purposes in the shade net of the shade density of lid plastics fogging-resistance film 80%, canopy
Fly net；Overhead kind of furrow bottom planting-line is built in canopy, with framework of the yoke as kind of planting-line, it is 0.3- then to lay aperture
0.5cm plastic board is as matrix support face, and furrow bottom overhead height is 30-50cm, then lays 8-12cm's in furrow face
Matrix；The matrix is by mashed potatoes 18kg, urea 4kg, phosphorus pentoxide 4kg, diammonium hydrogen phosphate 4kg, sodium tetraborate 3kg, meat
Osmanthus 18kg, Morinda officinalis 18kg, Chinese lobelia 28kg, Jiang Xia 23kg, coptis 23kg, tuber of dwarf lilyturf 18kg, dried rhizome of rehmannia 18kg, Fructus Corni 23kg,
Red sage root 23kg, American Ginseng 18kg, peach kernel 27kg, moutan bark 27kg and root of kudzu vine 27kg are prepared as follows：Take Chinese cassia tree, Morinda offcinalis How
My god, Chinese lobelia, Jiang Xia, the coptis, the tuber of dwarf lilyturf, the dried rhizome of rehmannia, Fructus Corni, the red sage root, American Ginseng, peach kernel, moutan bark and the root of kudzu vine, plus 8-12 times measured
Decocting boil after 3-5h, filter, take filter residue, after drying, be ground into coarse granule, coarse granule and mashed potatoes, urea, five oxidations two
Phosphorus, diammonium hydrogen phosphate and sodium tetraborate are mixed, and are produced；
(4) transplant：Transplanting time is March, and the deep duck eyes of 2-3cm are dug in the matrix of kind of planting-line, take dendrobium candidum to transplant
Seedling, is diluted after the sterilization of immersion root with 0.5% potassium permanganate, seedling root is put into duck eye, then covered with matrix, planted
Training density is 100-200 clumps/square metre, and every clump 2-5 plants, clump is away from for 5-10cm；
(5) Temperature and Humidity Control：Ensure that canopy temperature is maintained at 18-22 DEG C daytime, at 15-20 DEG C of night, humid control exists
80%-90%, 0.6% aqueous solution of urea is sprayed in 15 days after being transplanted after transplanting；
(6) rich water quality management：Control matrix normal moisture content in 30-40%, air humidity keeps 60%-70%, per 10-15
Its 1 water of spray, can spray 1 0.8% potassium dihydrogen phosphate aqueous solution in every 20 days；
(7) prevention and control of plant diseases, pest control：Monthly 1000 times of the 50% malathion aqueous solution sprays carry out foliar control, and insect prevention is using anti-
Worm net；
(8) harvest：Harvested between April November to next year, cut and stay small greatly, cut and stay tender always.
Embodiment 3.
A kind of cultural method of the high dendrobium candidum of stem of noble dendrobium alkali content, comprises the following steps：
(1) seedling is selected：Selection fleshy stem has 3~4 internodes, with 4~5 leaves, and long more than the 3cm of root has 4-5 bar roots, root
With green, no black root during color of the leather is white, no deformity, the dendrobium candidum seedling of no variation；
(2) seedling is handled：Dendrobium candidum seedling root is carried out after cleaning 3 times, seedling root is placed in 1000mg/L's
Soaked in heteroauxin after 20min, the dendrobium candidum seedling after must handling；
(3) greenhouse construction：Wide 6 meters, 28 meters of length is built, 110 meters of canopy shoulder height, the high 3min of ceiling planting greenhouse, ceiling covers
Running water pipe is installed, greenhouse surrounding and entrance are equipped with 30 purposes in the shade net of the shade density of lid plastics fogging-resistance film 70%, canopy
Fly net；Overhead kind of furrow bottom planting-line is built in canopy, with framework of the yoke as kind of planting-line, it is 0.3- then to lay aperture
0.5cm plastic board is as matrix support face, and furrow bottom overhead height is 30-50cm, then lays 8-12cm's in furrow face
Matrix；The matrix is by mashed potatoes 12kg, urea 2kg, phosphorus pentoxide 2kg, diammonium hydrogen phosphate 2kg, sodium tetraborate 2kg, meat
Osmanthus 12kg, Morinda officinalis 12kg, Chinese lobelia 22kg, Jiang Xia 17kg, coptis 17kg, tuber of dwarf lilyturf 12kg, dried rhizome of rehmannia 12kg, Fructus Corni 17kg,
Red sage root 17kg, American Ginseng 12kg, peach kernel 23kg, moutan bark 23kg and root of kudzu vine 23kg are prepared as follows：Take Chinese cassia tree, Morinda offcinalis How
My god, Chinese lobelia, Jiang Xia, the coptis, the tuber of dwarf lilyturf, the dried rhizome of rehmannia, Fructus Corni, the red sage root, American Ginseng, peach kernel, moutan bark and the root of kudzu vine, plus 8 times of amounts
Decocting is boiled after 3h, filtering, takes filter residue, after drying, and is ground into coarse granule, coarse granule and mashed potatoes, urea, phosphorus pentoxide, phosphorus
Sour diammonium hydrogen and sodium tetraborate are mixed, and are produced；
(4) transplant：Transplanting time is March, and the deep duck eyes of 2-3cm are dug in the matrix of kind of planting-line, take dendrobium candidum to transplant
Seedling, is diluted after the sterilization of immersion root with 0.3% potassium permanganate, seedling root is put into duck eye, then covered with matrix, planted
Training density is 100-200 clumps/square metre, and every clump 2-5 plants, clump is away from for 5-10cm；
(5) Temperature and Humidity Control：Ensure that canopy temperature is maintained at 18-22 DEG C daytime, at 15-20 DEG C of night, humid control exists
80%-90%, 0.4% aqueous solution of urea is sprayed in 10 days after being transplanted after transplanting；
(6) rich water quality management：Control matrix normal moisture content in 30-40%, air humidity keeps 60%-70%, every 10 days
1 water is sprayed, 1 0.5% potassium dihydrogen phosphate aqueous solution can be sprayed within every 15 days；
(7) prevention and control of plant diseases, pest control：Monthly sprayed with 40% dimethoate emulsifiable concentrate, 1000 times of aqueous solution and carry out foliar control, insect prevention is used
Fly net；
(8) harvest：Harvested between April November to next year, cut and stay small greatly, cut and stay tender always.
Claims (4)
1. a kind of cultural method of the high dendrobium candidum of stem of noble dendrobium alkali content, it is characterised in that：Comprise the following steps：
（1）Select seedling：Selection fleshy stem has 3 ~ 4 internodes, with 4 ~ 5 leaves, and long more than the 3cm of root has 4-5 bar roots, and root skin color is white
Middle band is green, no black root, no deformity, the dendrobium candidum seedling of no variation；
（2）Seedling is handled：Dendrobium candidum seedling root is carried out after cleaning 3-4 times, seedling root is placed in 1000mg/L Yin
Soaked in indolylbutyric acid after 20-30min, the dendrobium candidum seedling after must handling；
（3）Greenhouse is built：Build wide 6-8 meters, long 28-32 meters, 110-120 meters of canopy shoulder height, the high 3-4min of ceiling planting greenhouse,
Running water pipe, greenhouse surrounding and entrance dress are installed in the shade net of the good 70-80% shade densities of ceiling plastic covering fogging-resistance film, canopy
There is the fly net of 30-40 mesh；Overhead kind of furrow bottom planting-line is built in canopy, with framework of the yoke as kind of planting-line, hole is then laid
The plastic board that footpath is 0.3-0.5cm is as matrix support face, and furrow bottom overhead height is 30-50cm, is then laid in furrow face
8-12cm matrix；
（4）Transplant：Transplanting time is March, and the deep duck eyes of 2-3cm are dug in the matrix of kind of planting-line, take dendrobium candidum to transplant seedling,
After 0.3-0.5% potassium permanganate dilution immersion root sterilization, seedling root is put into duck eye, then covered with matrix, is planted
Training density is 100-200 clumps/square metre, and every clump 2-5 plants, clump is away from for 5-10cm；
（5）Temperature and Humidity Control：Ensure that canopy temperature is maintained at 18-22 DEG C daytime, at 15-20 DEG C of night, humid control is in 80%-
90%, spray within 10-15 days 0.4-0.6% aqueous solution of urea after being transplanted after transplanting；
（6）Rich water quality management：Control matrix normal moisture content in 30-40%, air humidity kept 60%-70%, per 10-15 days
1 water is sprayed, 1 0.5%~0.8% potassium dihydrogen phosphate aqueous solution can be sprayed per 15-20 days；
（7）The prevention and control of plant diseases, pest control：Monthly sprayed with 40% dimethoate emulsifiable concentrate, 1000 times of aqueous solution or 1 000 times of the 50% malathion aqueous solution
Carry out foliar control is applied, insect prevention uses fly net；
（8）Harvesting：Harvested between April November to next year, cut and stay small greatly, cut and stay tender always.
2. the cultural method of the high dendrobium candidum of stem of noble dendrobium alkali content as claimed in claim 1, it is characterised in that：The step
（3）In, main 1-5 parts of 1-5 parts of 10-20 parts of mashed potatoes, urea, phosphorus pentoxide, the phosphorus by calculating by weight of the matrix
Sour diammonium hydrogen 1-5 parts, 1-3 parts of sodium tetraborate, 10-20 parts of Chinese cassia tree, 10-20 parts of Morinda officinalis, 20-30 parts of Chinese lobelia, Jiang Xia 15-25
Part, 15-25 parts of the coptis, tuber of dwarf lilyturf 10-20 part, 10-20 parts of the dried rhizome of rehmannia, 15-25 parts of Fructus Corni, 15-25 parts of the red sage root, American Ginseng 10-20
Part, 20-30 parts of peach kernel, 20-30 parts of moutan bark and 20-30 parts of the root of kudzu vine are prepared as follows：Take Chinese cassia tree, Morinda officinalis, Chinese lobelia,
3- is boiled in Jiang Xia, the coptis, the tuber of dwarf lilyturf, the dried rhizome of rehmannia, Fructus Corni, the red sage root, American Ginseng, peach kernel, moutan bark and the root of kudzu vine, plus the 8-12 times of decocting measured
After 5h, filtering takes filter residue, after drying, and is ground into coarse granule, coarse granule and mashed potatoes, urea, phosphorus pentoxide, phosphoric acid hydrogen two
Amine and sodium tetraborate are mixed, and are produced.
3. the cultural method of the high dendrobium candidum of stem of noble dendrobium alkali content as claimed in claim 2, it is characterised in that：The matrix master
Will by calculate by weight 2-4 parts of 2-4 parts of 2-4 parts of 12-18 parts of mashed potatoes, urea, phosphorus pentoxide, diammonium hydrogen phosphate, four
2-3 parts of Boratex, 12-18 parts of Chinese cassia tree, 12-18 parts of Morinda officinalis, 22-28 parts of Chinese lobelia, 17-23 parts of Jiang Xia, 17-23 parts of the coptis,
Tuber of dwarf lilyturf 12-18 part, 12-18 parts of the dried rhizome of rehmannia, 17-23 parts of Fructus Corni, 17-23 parts of the red sage root, 12-18 parts of American Ginseng, 23-27 parts of peach kernel,
23-27 parts of 23-27 parts of moutan bark and the root of kudzu vine are prepared as follows：Take Chinese cassia tree, Morinda officinalis, Chinese lobelia, Jiang Xia, the coptis, the tuber of dwarf lilyturf,
The dried rhizome of rehmannia, Fructus Corni, the red sage root, American Ginseng, peach kernel, moutan bark and the root of kudzu vine, plus the 8-12 times of decocting measured are boiled after 3-5h, filtering, take filter
Slag, after drying, is ground into coarse granule, coarse granule is mixed with mashed potatoes, urea, phosphorus pentoxide, diammonium hydrogen phosphate and sodium tetraborate
It is even, produce.
4. the cultural method of the high dendrobium candidum of stem of noble dendrobium alkali content as claimed in claim 3, it is characterised in that：The matrix master
Will be by 3 parts of 3 parts of 3 parts of 15 parts of mashed potatoes, urea, phosphorus pentoxide, the diammonium hydrogen phosphate, the sodium tetraborate 2.5 that calculate by weight
Part, 15 parts of Chinese cassia tree, 15 parts of Morinda officinalis, 25 parts of Chinese lobelia, 20 parts of Jiang Xia, 20 parts of the coptis, 15 parts of the tuber of dwarf lilyturf, 15 parts of the dried rhizome of rehmannia, Fructus Corni
25 parts of 20 parts, 20 parts of the red sage root, 15 parts of American Ginseng, 25 parts of peach kernel, 25 parts of moutan bark and the root of kudzu vine are prepared as follows：Take Chinese cassia tree,
Morinda officinalis, Chinese lobelia, Jiang Xia, the coptis, the tuber of dwarf lilyturf, the dried rhizome of rehmannia, Fructus Corni, the red sage root, American Ginseng, peach kernel, moutan bark and the root of kudzu vine, plus 8-12
The decocting of amount is boiled after 3-5h again, is filtered, and takes filter residue, after drying, and is ground into coarse granule, and coarse granule is aoxidized with mashed potatoes, urea, five
Two phosphorus, diammonium hydrogen phosphate and sodium tetraborate are mixed, and are produced.
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