CN107567971A - A kind of pseudo-wild cultivating method of dendrobium candidum - Google Patents
A kind of pseudo-wild cultivating method of dendrobium candidum Download PDFInfo
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- CN107567971A CN107567971A CN201710960226.XA CN201710960226A CN107567971A CN 107567971 A CN107567971 A CN 107567971A CN 201710960226 A CN201710960226 A CN 201710960226A CN 107567971 A CN107567971 A CN 107567971A
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Abstract
The invention discloses a kind of pseudo-wild cultivating method of dendrobium candidum, including germination seed to sprout：Take Seeds of Dendrobium Candidum, after cleaning, after 70 80% 15min of ethanol disinfection 5 is used on superclean bench, aseptic water washing is used again 34 times, be placed on the filter paper of sterilizing after blotting, be inoculated on germination medium, it it is 20 25 DEG C in daytime temperature, under the conditions of 18 20 DEG C of night temperature, light culture, until seed is sprouted；Seedling culture：Treat that seed is sprouted, grow into 3 4cm it is high when, when having 35 adventitious roots, remove, be positioned over culture 10 15 days in greenhouse, then culture 79 days is continued in outdoor, obtains dendrobium candidum seedling；Pseudo-wild cultivating：Select canopy density for 0.6 0.7 the ecological forest, using trunk as " ", one Cong Cong dendrobium candidums seedling is tied up in tree trunk from top to down with wide 8 10cm environmentally friendly numb net, numb rope, pasture and water or moss, every plant of tree binding 5O 100 clumps, automatic Fine spray shower nozzle is installed, relative air humidity is controlled in 6O 8O%；After transplanting 15 18 months, harvesting.
Description
Technical field
The present invention relates to a kind of cultural method of dendrobium candidum, the extraction of the high dendrobium candidum of particularly a kind of polyoses content
Method.
Background technology
Dendrobium candidum nickname iron sheet orchid, bracketplant, Yunnan iron sheet, iron sheet bracketplant, it is orchid family Dendrobium herbaceos perennial,
Dendrobium candidum is the top grade of Shihu " medicinal materials, is known as the laudatory title of " gold in Chinese celestial grass-medicine ", is used as medicine with stem, contains polysaccharide, stone
The active ingredients such as dry measure used in former times alkali, alkaloid, with nourishing Yin and clearing heat, the beneficial stomach that promotes the production of body fluid, wet one's whistle improving eyesight and other effects, in antitumor, raising human body
Immunity, treatment enterogastric diseases, anti-aging, anti-oxidant, platelet aggregation-against, reduction blood glucose, treatment cataract etc. are
There is good efficacy.
Modern pharmacology research shows that dendrobium candidum has humidification to non-specific and specific immunity, anti-swollen
Knurl, treatment that is anti-oxidant, reducing blood glucose and cataract etc., pharmacological action is notable.Its chemical composition is more complicated, it has been determined that
Type of compounds mainly have：Alkaloids, polysaccharide, luxuriant and rich with fragrance class, bibenzyl parent nucleus class, wherein alkaloids, polysaccharide pharmacology are lived
Property is more notable.Polysaccharide constituents are that medicinal dendrobium plays the important substance basis that immunocompetence acts on, Dendrobium officinale polysaccharide energy
Peripheral leukocytes are significantly improved, promotes lymphocyte to produce migration inhibition factor, immunity of organisms can be effectively improved.2010 editions
《Pharmacopeia》The quality standard of dendrobium candidum has been recorded first, and assay is carried out to polysaccharide and mannose.
As the health-care efficacy of dendrobium candidum by people is widely recognized and received, dendrobium officinale far can not expire
The demand of sufficient people.At present, the dendrobium candidum of in the market circulation is substantially obtained by artificial growth, as greenhouse facility is planted
The curing and popularization and application of training technology, the cultivated area and yield of dendrobium candidum also expand rapidly, but planting household is quickening iron sheet
The speed of growth and increase yield of the stem of noble dendrobium, apply a large amount of fertilizer and pesticides, the dendrobium candidum that this mode is planted in planting process
Often have that poor quality, active ingredient is relatively low, particularly the polyoses content in dendrobium candidum is low.
The content of the invention
The purpose of the present invention, it is to provide a kind of pseudo-wild cultivating method of dendrobium candidum, when the inventive method plants dendrobium candidum,
The dendrobium candidum quality better finally plucked, active constituent content is high, particularly the height of the content of polysaccharide.
What the present invention was realized in.A kind of pseudo-wild cultivating method of dendrobium candidum, including tissue culture propagating and imitative
Wild cultivating.
In the pseudo-wild cultivating method of foregoing dendrobium candidum, following steps are specifically included：
（1）Seed is sprouted：Seeds of Dendrobium Candidum is taken, after cleaning, 70-80% ethanol disinfection 5- is used on superclean bench
After 15min, then with aseptic water washing 3-4 times, it is placed on the filter paper of sterilizing after blotting, is inoculated on germination medium, in daytime temperature
For 20-25 DEG C, under the conditions of 18-20 DEG C of night temperature, light culture, until seed is sprouted；
（2）Seedling culture：Treat seed sprout, grow into 3-4cm it is high when, when having 3-5 bar adventitious roots, remove, be positioned in greenhouse
Culture 10-15 days, then outdoor continue culture 7-9 days, obtain dendrobium candidum seedling；
（3）Pseudo-wild cultivating：Select canopy density for 0.6-0.7 the ecological forest, using trunk as " ", by a Cong Cong dendrobium candidums
Seedling is tied up in tree trunk from top to down with wide 8-10cm environmentally friendly numb net, numb rope, pasture and water or moss, every plant of tree binding 5O-
100 clumps, automatic Fine spray shower nozzle is installed, relative air humidity is controlled in 6O-8O%；After transplanting 15-18 months, harvesting.
In the pseudo-wild cultivating method of foregoing dendrobium candidum, the step（1）In, the germination medium mainly by by
Sucrose 3-6 parts of listed as parts by weight, sweet potato mash 10-20 parts, soybean residue 5-15 parts, Polygonum multiflower knotweed 5-15 parts, radix bupleuri 10-20 parts, Huang
Even 5-15 parts, subprostrate sophora 5-15 parts, bighead atractylodes rhizome 10-20 parts and Chinese ephedra 10-20 parts are made as follows：Polygonum multiflower knotweed, radix bupleuri, Huang
After company, subprostrate sophora, the bighead atractylodes rhizome and Chinese ephedra add the 10-12 times of decocting measured to boil 2-3h, filtering, filter residue is taken, dry, pulverize into coarse granule,
Coarse granule mixes with sucrose, sweet potato mash, soybean residue, produces.
In the pseudo-wild cultivating method of foregoing dendrobium candidum, the main sugarcane by calculating by weight of the germination medium
Sugared 4-5 parts, sweet potato mash 13-17 parts, soybean residue 7-13 parts, night hand over 7-13 parts, radix bupleuri 12-18 parts, coptis 7-13 parts, subprostrate sophora 7-
13 parts, bighead atractylodes rhizome 12-18 parts and Chinese ephedra 12-18 parts are made as follows：Polygonum multiflower knotweed, radix bupleuri, the coptis, subprostrate sophora, the bighead atractylodes rhizome and fiber crops
After 2-3h is boiled in Huang plus the 10-12 times of decocting measured, filtering, filter residue is taken, dry, pulverize into coarse granule, coarse granule and sucrose, sweet potato
Mud, soybean residue mix, and produce.
In the pseudo-wild cultivating method of foregoing dendrobium candidum, the main sugarcane by calculating by weight of the germination medium
5 parts of sugar, 15 parts of sweet potato mash, 10 parts of soybean residue, 10 parts of Polygonum multiflower knotweed, 15 parts of radix bupleuri, 10 parts of the coptis, 10 parts of subprostrate sophora, 15 parts of the bighead atractylodes rhizome
15 parts are made as follows with Chinese ephedra：Polygonum multiflower knotweed, radix bupleuri, the coptis, subprostrate sophora, the bighead atractylodes rhizome and Chinese ephedra add the decocting of 11 times of amounts to boil
After 2.5h, filtering, filter residue is taken, dry, pulverize into coarse granule, coarse granule is mixed with sucrose, sweet potato mash, soybean residue, produced.
Applicant has carried out substantial amounts of experimental study to the present invention, and part Experiment is as follows：
The polyoses content of experimental example 1 investigates
1 materials and methods
1.1 material
1.1.1 dendrobium candidum 1：Planted by embodiment 1, pluck, produce.
Dendrobium candidum 2：Planted by embodiment 1, be free of the red sage root and the tuber of dwarf lilyturf in germination medium, in germination medium not
Containing Polygonum multiflower knotweed, radix bupleuri, the coptis, subprostrate sophora, the bighead atractylodes rhizome and Chinese ephedra, remaining method is the same as embodiment 1.
Instrument and reagent
Dextrose standard sample [glucose of D mono- (+) one]：KAYON companies；Methanol (chromatographically pure)：Close europeanized learn of Tianjin section is tried
Agent Co., Ltd；Remaining reagent is that analysis is pure；Phenol：Shanghai Ling Feng chemical reagent Co., Ltd；Sulfuric acid：Shanghai Ling Feng chemistry
Reagent Co., Ltd.
Method
The preparation of 2.1 need testing solutions
2.1.1 the preparation of need testing solution 1：
Take dendrobium candidum of the present invention（Dendrobium candidum 1）Sample, 50 DEG C are put in baking oven, dry, pulverize, No. three sieves are crossed, after taking filtering
Medicinal powder, solvent is volatilized, add the mL of water 2000, be heated to reflux 2 hours, let cool, be transferred in 250 mL measuring bottles, with a small amount of
Moisture time washing container, washing lotion are incorporated in same measuring bottle, add water to scale, shake up, and filter, and precision measures the mL of subsequent filtrate 2, put
In 15 mL centrifuge tubes, precision adds 95% ethanol, shakes up, and refrigerates 1 hour, takes out, and centrifuges 20 minutes, abandoning supernatant, sinks
Form sediment and add hot water dissolving, be transferred in 25 mL measuring bottles, let cool, add water to scale, shake up, produce need testing solution 1.
The preparation of need testing solution 2
The sample of dendrobium candidum 2 is taken, 50 DEG C is put in baking oven, dry, pulverize, No. three sieves is crossed, takes medicinal powder after filtering, volatilize molten
Agent, add the mL of water 2000, be heated to reflux 2 hours, let cool, be transferred in 250 mL measuring bottles, with a small amount of moisture time washing container,
Washing lotion is incorporated in same measuring bottle, adds water to scale, is shaken up, and filtration, precision measures the mL of subsequent filtrate 2, put in 15 mL centrifuge tubes, essence
95% ethanol of close addition, shakes up, and refrigerates 1 hour, takes out, and centrifuges 20 minutes, abandoning supernatant, precipitation plus hot water dissolving, turns
Move in 25 mL measuring bottles, let cool, add water to scale, shake up, produce need testing solution 2.
The preparation of reference substance solution
It is appropriate to the DEXTROSE ANHYDROUS of constant weight that precision weighs 105 DEG C of dryings, adds water that 99 mg/L solution are made, standby.
It is prepared by standard curve
Precision measures the m L of reference substance solution 0.2,0.4,0.6,0.8,1.0 and put in 10m L tool plug test tubes, adds water to
1.0mL, precision add 5% phenol solution 1mL (I is now prepared before use), shaken up, then the accurate addition m L of sulfuric acid 5, shake up, put
Heated 20 minutes in boiling water bath, take out, be put into ice-water bath and cool down 5 minutes, using corresponding reagent as blank, in 488 nm wavelength
Place's measure absorbance, using absorbency Y as ordinate, concentration X (g/L) is abscissa, draws standard curve, and regression equation is：Y
=11.772X -0.1492, r=0.9995.As a result show that polysaccharide linear relationship in the range of 0.02-0.10 g/L is good
It is good.
Determination method
Precision measures need testing solution 1 and each 0.5 mL of need testing solution 2 respectively, puts in 10 mL tool plug test tubes, adds water to 1mL
, by the method under the preparation of " 2.3 " standard curve, from " precision adds 5% phenol solution 1m L ", determining extinction in accordance with the law
Degree, the content of DEXTROSE ANHYDROUS in need testing solution is read from standard curve, calculate, produce.
Precision test
Precision draws 6 parts, every part of 0.6 m L of glucose control product solution, and absorbance is determined according to the method under " 2.4 " item.Survey
Determine result, RSD is respectively 1.60% and 1.62%, and precision is good.
Replica test
6 parts of need testing solutions 1 and need testing solution 2 are prepared as stated above, according to determining absorbance as stated above.Measure knot
Fruit, RSD are respectively 2.70% and 2.71%, and repeatability is good.
Stability test
Same need testing solution 1 and need testing solution 2 are taken respectively, is placed at room temperature, respectively at 10min, 30min, 1 h, 2
H, absorbance is determined as stated above after 4 h, 6 h.Measurement result, RSD are respectively 2.70% and 2.73%, show that test sample is molten
Liquid 1 and need testing solution 2 are stable in 6 hours.
Average recovery is tested
6 parts, every part of about 1.65g of 1 powder of dendrobium candidum of known content (12.5%) is weighed, it is accurately weighed.It is accurate to add grape
2 mL of sugared reference substance solution (concentration is 0.099 g/L), prepare need testing solution 1, determine as stated above as stated above,
Polyoses content is calculated, as a result average recovery rate is 94.9%, RSD 2.7%.
6 parts, every part of about 1.65g of 2 powder of dendrobium candidum of known content (12.5%) is weighed, it is accurately weighed.It is accurate to add
Glucose control product solution (concentration is 0.099 g/L) 2 mL, prepare need testing solution 2, survey as stated above as stated above
It is fixed, polyoses content is calculated, as a result average recovery rate is 93.9%, RSD 2.6%.
Sample determines
By the preparation method and condition determination of above-mentioned need testing solution 1 and need testing solution 2, determine 6 parts of samples of dendrobium candidum 1 and
The content of polysaccharide in 6 parts of samples of dendrobium candidum 2, result is recorded, results averaged, the results are shown in Table 1.
As seen from table, the inventive method cultivates obtained dendrobium candidum, the content of polysaccharide in the dendrobium candidum finally harvested
It is higher.The quality better of dendrobium candidum.
Compared with prior art, during the inventive method plantation dendrobium candidum, the dendrobium candidum quality better finally plucked, effectively
Component content is high, particularly the height of the content of polysaccharide.
Embodiment
Embodiment 1.
A kind of pseudo-wild cultivating method of dendrobium candidum, specifically includes following steps：
（1）Seed is sprouted：Seeds of Dendrobium Candidum is taken, after cleaning, after 75% ethanol disinfection 10min is used on superclean bench,
Aseptic water washing is used again 3 times, is placed on the filter paper of sterilizing after blotting, is inoculated on germination medium, be 20-25 DEG C in daytime temperature,
Under the conditions of 18-20 DEG C of night temperature, light culture, until seed is sprouted；
（2）Seedling culture：Treat seed sprout, grow into 3-4cm it is high when, when having 3-5 bar adventitious roots, remove, be positioned in greenhouse
Culture 12 days, then outdoor continue culture 8 days, obtain dendrobium candidum seedling；
（3）Pseudo-wild cultivating：Select canopy density for 0.6-0.7 the ecological forest, using trunk as " ", by a Cong Cong dendrobium candidums
Seedling is tied up in tree trunk from top to down with wide 8-10cm environmentally friendly numb net, numb rope, pasture and water or moss, every plant of tree binding 5O-
100 clumps, automatic Fine spray shower nozzle is installed, relative air humidity is controlled in 6O-8O%；After plant 17 months, harvesting.
The germination medium is by sucrose 5kg, sweet potato mash 15kg, soybean residue 10kg, Polygonum multiflower knotweed 10kg, radix bupleuri 15kg, Huang
Even 10kg, subprostrate sophora 10kg, bighead atractylodes rhizome 15kg and Chinese ephedra 15kg are made as follows：It is Polygonum multiflower knotweed, radix bupleuri, the coptis, subprostrate sophora, white
After art and Chinese ephedra add the decoctings of 11 times of amounts to boil 2.5h, filtering, filter residue is taken, dry, pulverize into coarse granule, it is coarse granule and sucrose, red
Mashed potatoes, soybean residue mix, and produce.
Embodiment 2.
A kind of pseudo-wild cultivating method of dendrobium candidum, specifically includes following steps：
（1）Seed is sprouted：Seeds of Dendrobium Candidum is taken, after cleaning, after 80% ethanol disinfection 15min is used on superclean bench,
Aseptic water washing is used again 4 times, is placed on the filter paper of sterilizing after blotting, is inoculated on germination medium, be 20-25 DEG C in daytime temperature,
Under the conditions of 18-20 DEG C of night temperature, light culture, until seed is sprouted；
（2）Seedling culture：Treat seed sprout, grow into 3-4cm it is high when, when having 3-5 bar adventitious roots, remove, be positioned in greenhouse
Culture 15 days, then outdoor continue culture 9 days, obtain dendrobium candidum seedling；
（3）Pseudo-wild cultivating：Select canopy density for 0.6-0.7 the ecological forest, using trunk as " ", by a Cong Cong dendrobium candidums
Seedling is tied up in tree trunk from top to down with wide 8-10cm environmentally friendly numb net, numb rope, pasture and water or moss, every plant of tree binding 5O-
100 clumps, automatic Fine spray shower nozzle is installed, relative air humidity is controlled in 6O-8O%；After plant 18 months, harvesting.
The germination medium hands over 13kg, radix bupleuri 18kg, the coptis by sucrose 5kg, sweet potato mash 17kg, soybean residue 13kg, night
13kg, subprostrate sophora 13kg, bighead atractylodes rhizome 18kg and Chinese ephedra 18kg are made as follows：Polygonum multiflower knotweed, radix bupleuri, the coptis, subprostrate sophora, the bighead atractylodes rhizome
After 3h is boiled in the decoctings measured with Chinese ephedra plus 12 times, filtering, take filter residue, dry, pulverize into coarse granule, coarse granule and sucrose, sweet potato mash,
Soybean residue mixes, and produces.
Embodiment 3.
A kind of pseudo-wild cultivating method of dendrobium candidum, specifically includes following steps：
（1）Seed is sprouted：Seeds of Dendrobium Candidum is taken, after cleaning, after 70% ethanol disinfection 5min is used on superclean bench,
Aseptic water washing is used again 3 times, is placed on the filter paper of sterilizing after blotting, is inoculated on germination medium, be 20-25 DEG C in daytime temperature,
Under the conditions of 18-20 DEG C of night temperature, light culture, until seed is sprouted；
（2）Seedling culture：Treat seed sprout, grow into 3-4cm it is high when, when having 3-5 bar adventitious roots, remove, be positioned in greenhouse
Culture 10 days, then outdoor continue culture 7 days, obtain dendrobium candidum seedling；
（3）Pseudo-wild cultivating：Select canopy density for 0.6-0.7 the ecological forest, using trunk as " ", by a Cong Cong dendrobium candidums
Seedling is tied up in tree trunk from top to down with wide 8-10cm environmentally friendly numb net, numb rope, pasture and water or moss, every plant of tree binding 5O-
100 clumps, automatic Fine spray shower nozzle is installed, relative air humidity is controlled in 6O-8O%；After plant 15 months, harvesting.
The germination medium hands over 7kg, radix bupleuri 12kg, the coptis by sucrose 4kg, sweet potato mash 13kg, soybean residue 7kg, night
7kg, subprostrate sophora 7kg, bighead atractylodes rhizome 12kg and Chinese ephedra 12kg are made as follows：Polygonum multiflower knotweed, radix bupleuri, the coptis, subprostrate sophora, the bighead atractylodes rhizome and
After Chinese ephedra adds the decocting of 10 times of amounts to boil 2h, filtering, filter residue is taken, dry, pulverize into coarse granule, coarse granule and sucrose, sweet potato mash, Huang
Bean dregs mix, and produce.
Claims (5)
- A kind of 1. pseudo-wild cultivating method of dendrobium candidum, it is characterised in that：Including tissue culture propagating and pseudo-wild cultivating.
- 2. the pseudo-wild cultivating method of dendrobium candidum as claimed in claim 1, it is characterised in that：Specifically include following steps：Seed is sprouted：Seeds of Dendrobium Candidum is taken, after cleaning, 70-80% ethanol disinfection 5-15min is used on superclean bench Afterwards, then with aseptic water washing 3-4 times, it is placed on the filter paper of sterilizing after blotting, is inoculated on germination medium, be 20- in daytime temperature 25 DEG C, under the conditions of 18-20 DEG C of night temperature, light culture, until seed is sprouted；Seedling culture：Treat that seed is sprouted, grow into 3-4cm it is high when, when having 3-5 bar adventitious roots, remove, be positioned in greenhouse and cultivate 10-15 days, then outdoor continue culture 7-9 days, obtain dendrobium candidum seedling；（3）Pseudo-wild cultivating：Select canopy density for 0.6-0.7 the ecological forest, using trunk as " ", by a Cong Cong dendrobium candidums Seedling is tied up in tree trunk from top to down with wide 8-10cm environmentally friendly numb net, numb rope, pasture and water or moss, every plant of tree binding 5O- 100 clumps, automatic Fine spray shower nozzle is installed, relative air humidity is controlled in 6O-8O%；After transplanting 15-18 months, harvesting.
- 3. the pseudo-wild cultivating method of dendrobium candidum as claimed in claim 2, it is characterised in that：The step（1）In, it is described Germination medium mainly by calculate by weight sucrose 3-6 parts, sweet potato mash 10-20 parts, soybean residue 5-15 parts, Polygonum multiflower knotweed 5-15 Part, radix bupleuri 10-20 parts, coptis 5-15 parts, subprostrate sophora 5-15 parts, bighead atractylodes rhizome 10-20 parts and Chinese ephedra 10-20 parts are made as follows Into：After Polygonum multiflower knotweed, radix bupleuri, the coptis, subprostrate sophora, the bighead atractylodes rhizome and Chinese ephedra add the 10-12 times of decocting measured to boil 2-3h, filtering, filter residue is taken, done It is dry, coarse granule is ground into, coarse granule is mixed with sucrose, sweet potato mash, soybean residue, produced.
- 4. the pseudo-wild cultivating method of dendrobium candidum as claimed in claim 3, it is characterised in that：The germination medium is main By sucrose 4-5 parts, sweet potato mash 13-17 parts, soybean residue 7-13 parts, night for calculating by weight hand over 7-13 parts, radix bupleuri 12-18 parts, Coptis 7-13 parts, subprostrate sophora 7-13 parts, bighead atractylodes rhizome 12-18 parts and Chinese ephedra 12-18 parts are made as follows：Polygonum multiflower knotweed, radix bupleuri, Huang After company, subprostrate sophora, the bighead atractylodes rhizome and Chinese ephedra add the 10-12 times of decocting measured to boil 2-3h, filtering, filter residue is taken, dry, pulverize into coarse granule, Coarse granule mixes with sucrose, sweet potato mash, soybean residue, produces.
- 5. the pseudo-wild cultivating method of dendrobium candidum as claimed in claim 4, it is characterised in that：The germination medium is main By calculate by weight 5 parts of sucrose, 15 parts of sweet potato mash, 10 parts of soybean residue, 10 parts of Polygonum multiflower knotweed, 15 parts of radix bupleuri, 10 parts of the coptis, mountain 15 parts of 10 parts of beans root, 15 parts of the bighead atractylodes rhizome and Chinese ephedra are made as follows：Polygonum multiflower knotweed, radix bupleuri, the coptis, subprostrate sophora, the bighead atractylodes rhizome and Chinese ephedra After adding the decocting of 11 times of amounts to boil 2.5h, filtering, filter residue is taken, dry, pulverize into coarse granule, coarse granule and sucrose, sweet potato mash, soya bean Slag mixes, and produces.
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| CN108079198A (en) * | 2018-01-18 | 2018-05-29 | 贵州独秀峰药业有限公司 | A kind of extraction process of dendrobium candidum |
| CN108782199A (en) * | 2018-05-19 | 2018-11-13 | 安徽省霍山县霍鼎仙石斛开发有限公司 | A kind of implantation methods of growing nonparasitically upon another plant of the stem of noble dendrobium |
| CN108782199B (en) * | 2018-05-19 | 2021-06-15 | 安徽省霍山县霍鼎仙石斛开发有限公司 | Epiphytic planting method for dendrobium |
| CN110272505A (en) * | 2019-07-22 | 2019-09-24 | 云南农业大学 | A kind of dendrobium candidum Thick many candies extracting method and polyoses content detection method |
| CN117099665A (en) * | 2023-08-16 | 2023-11-24 | 广西壮族自治区农业科学院 | Method for cultivating dendrobium candidum by ecologically returning rock wall |
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