CN102499038A - Tree tissue culture method of dendrobium candidum - Google Patents
Tree tissue culture method of dendrobium candidum Download PDFInfo
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- CN102499038A CN102499038A CN2011103420542A CN201110342054A CN102499038A CN 102499038 A CN102499038 A CN 102499038A CN 2011103420542 A CN2011103420542 A CN 2011103420542A CN 201110342054 A CN201110342054 A CN 201110342054A CN 102499038 A CN102499038 A CN 102499038A
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Abstract
The invention discloses a tree tissue culture method of dendrobium candidum, which belongs to the agriculture technical field, and aims at solving the technical problem that a tree tissue culture method of the dendrobium candidum is provided, and belongs to the bionics wild cultivation. In order to solve the problems, the tissue culture method of the dendrobium candidum comprises the following steps that: 1) a tree with surface of a tree bark having grains is selected as an epiphytic tree, a dendrobium candidum seedling after being domesticated is selected as an epiphytic seedling, and a hay band is selected as a binding rope; 2) the epiphytic seedling is arranged on the surface of a trunk of the epiphytic tree, and root of the epiphytic seedling is wound by the binding rope to be fixed; and 3) the tree tissue culture humidity is controlled at 70 percent to 85 percent. Due to the adoption of the method, the dendrobium candidum can be planted on the tree with the surface having the grains.
Description
Technical field
The present invention relates to a kind of cultivation method of dendrobium candidum, belong to agricultural technology field.
Background technology
Dendrobium candidum, herbaceous plant grows nonparasitically upon another plant for the orchid family is perennial.Be born in height above sea level and reach on the dark and damp rock in 1600 meters mountain region half, the environment of happiness warm and moist weather and half cloudy half light, cold-resistant.The low temperature of general equal ability-5 ℃.The stem of noble dendrobium can be divided into tens of kinds on yellow grass, Jin Chai, horsewhip etc., and dendrobium candidum is the superfine product of the stem of noble dendrobium, and iron is green gains the name because of epidermis is for it.Dendrobium candidum has unique medical value, is used as medicine the Chinese medicine name with its stem: the stem of noble dendrobium, belong to the yin tonics in the tonic, " Chinese pharmacopoeia: reinforcing stomach reg fluid is nourishing Yin and clearing heat.The tender stem of minority kinds such as dendrobium candidum is twisted into helical form or spring-like, dries, and commodity are called earrings dendrobium, has another name called the maple bucket.
According to the selling price of dendrobium candidum in second half 2010 Zhejiang Province, wild dendrobium candidum fresh goods are 8 times of dendrobium candidum fresh goods of plantation, the wild stem of noble dendrobium resource growing tension that more seems, and supply and demand is more particularly thorny.Therefore, we imitate the cultivation of wild dendrobium candidum, and it is significant, has a extensive future.This be in the agriculture industrialization one advanced, strategic, basic exploration project is arranged, help adapting to better market and guiding market comsupton, drive the benign development of Chinese medicine and health products industry.
Using the most at present, the traditional cultivation method mainly contains land for growing field crops greenhouse cultivation technology.The cost of booth is high in the greenhouse cultivation method of land for growing field crops, and one mu of booth cost is low by then three or five ten thousand, then surplus in the of 20 ten thousand yuan of height, and the input in early stage is too big, is unfavorable for promoting the use of of peasant.In addition, the environment in booth land for growing field crops is prone to endophytic bacteria property and fungoid illness.Because dendrobium candidum happiness is moist, water funk again too much water is many, and poor aeration can be rotted root; Water is few, and overdrying can thirst again, though the stem of noble dendrobium has stronger drought resistance; But long-term lack of water, the too high meeting of surface temperature is roasting dead, wet excessively stem of noble dendrobium base portion; Overlong time, root will be rotted extremely, and the growth animated period is afraid of arid again and is afraid of ponding.The environment that adds the land for growing field crops is easy to generate more damage by disease and insect, and these all are to cause bacillary and the reason fungoid illness.Therefore, the peasant needs higher technology and more rich experience when control, and this also is unfavorable for promoting the use of of peasant.
The land for growing field crops greenhouse cultivation is in the process of plantation, owing to lacked natural environment, the dendrobium candidum that field planting comes out has also lacked the distinctive fragrance of wild dendrobium candidum at the stem of noble dendrobium crude polysaccharides content that is difficult on the quality reach more than 15% on the mouthfeel.In addition, in frying in shallow oil the process of bubble, the dendrobium candidum of field planting can't reach the purpose of frying in shallow oil bubble for several times.In a word, the dendrobium candidum of field planting greatly reduces the effect of the stem of noble dendrobium.
Summary of the invention
The technical problem that the present invention will solve provides a kind of tree culture method of dendrobium candidum, is imitating wild planting.
For addressing the above problem, candidum tissue culturing method of the present invention comprises the following steps:
1) selects the veined seeds in bark surface to be the tree that grows nonparasitically upon another plant, select to be the seedling of growing nonparasitically upon another plant, select straw rope for tying up rope through the dendrobium candidum seedling after the domestication;
2) seedling of will growing nonparasitically upon another plant places the trunk surface of the tree that grows nonparasitically upon another plant, and a few are fixed with tying up the rope winding at the root place of the seedling of growing nonparasitically upon another plant;
3) tree training humidity is controlled at 70-85%.
The tree culture method of described a kind of dendrobium candidum is characterized in that step 2) described in the tree that grows nonparasitically upon another plant be the camphor tree of close grain or cypress.
The tree culture method of described a kind of dendrobium candidum is characterized in that step 2) described in liftoff at least 1 meter of the seedling of growing nonparasitically upon another plant, every interval 30-50cm is provided with the circle seedling of growing nonparasitically upon another plant.
The tree culture method of described a kind of dendrobium candidum is characterized in that step 2) described in tie up rope to twine the number of turns be the 3-5 circle.
The tree culture method of described a kind of dendrobium candidum is characterized in that step 2) described in tie up the rope cross-sectional diameter be 1-2cm.
The tree culture method of described a kind of dendrobium candidum is characterized in that step 2) described in twine with tying up rope at the root place of the seedling of growing nonparasitically upon another plant, its concrete winding position is a coring portion, exposes foundation portion.
The tree culture method of described a kind of dendrobium candidum is characterized in that selecting annual mid-April to being the field planting time by the end of June.
The tree culture method of described a kind of dendrobium candidum is characterized in that the training of tree described in step 3) humidity is controlled at 75%.
Dendrobium candidum belongs to the herbaceous plant that grows nonparasitically upon another plant; Its root mass is gone up growth attached to the limb of other tree; Utilize the humus (residual leaf of rotten deadwood or animal excrements etc.) on rain and dew, airborne steam and limb surface to make a living, because the particularity that dendrobium candidum requires growing environment, so under natural environment; It is considerably less to meet dendrobium candidum growth trees environment, and this also is the wild rare main cause of dendrobium candidum.The inventor utilizes dendrobium candidum this habit of growing nonparasitically upon another plant on other tree, through experiment in a large number, sums up the tree culture method that draws the effective dendrobium candidum of a cover, can be implemented on the veined seeds of all surface through the present invention and plant dendrobium candidum.
The present invention utilizes straw rope will grow nonparasitically upon another plant the seedling field planting on limb; Straw rope can effectively store required moisture of dendrobium candidum seedling growth and the ventilative requirement of root; Straw rope can slowly be degenerated simultaneously, discharges dendrobium candidum and grows needed foster part, in addition through the selection to the tree that grows nonparasitically upon another plant, the seedling of growing nonparasitically upon another plant; The control of humidity, these one-tenth to dendrobium candidum are lived and are all played crucial effects.
Also reduce the loss that causes because of lack of water or ponding through the present invention, guaranteed the needs of dendrobium candidum half dark and damp environment.Simultaneously, reduce the generation of damage by disease and insect, thereby guaranteed the excellence of quality and mouthfeel.Plantation of the present invention need not soil, need not fertile, not medication, and the dendrobium candidum crude polysaccharides content that in such wild environment, plants is more much higher than land for growing field crops greenhouse gardening, and planting cost is lower, helps the plantation of vast farmers.And the sylvan life resource of China can't be utilized mostly, and this method utilizes the sylvan life natural conditions in vast mountain area to be the basis, utilizes science and technology to drive local economic development and is starting point, carry out the dendrobium candidum industrialization and produce, and be the trend of meeting the market requirement.
Description of drawings
Below in conjunction with accompanying drawing and embodiment the present invention is done further detailed explanation.
Fig. 1 be the dendrobium candidum seedling that will tame with straw rope with the state diagram of its field planting on limb.
The dendrobium candidum seedling of Fig. 2 for taming.
Wherein, 1-straw rope, 2-trunk, 3-tree line, 4-grow nonparasitically upon another plant seedling, H1-grow nonparasitically upon another plant seedling spacing, H2-grow nonparasitically upon another plant the seedling ground clearance, that A-ties up the rope winding position is interval.
Embodiment
Embodiment 1:
As depicted in figs. 1 and 2, the tree culture method of dendrobium candidum of the present invention is characterized in that this tree culture method comprises the steps:
1) select the veined seeds in bark surface to be the tree that grows nonparasitically upon another plant; The dendrobium candidum seedling that selection was tamed (refining seedling) through 1 year left and right sides time is the seedling of growing nonparasitically upon another plant; Select straw rope for tying up rope, said straw rope is to be the straw-made articles that raw material is processed through special-purpose straw-rope machine or artificial braiding forms with the rice straw;
2) many seedlings of growing nonparasitically upon another plant are placed the trunk surface of the tree that grows nonparasitically upon another plant, on trunk, are circumferential arrangement, then at the root place of the seedling of growing nonparasitically upon another plant with tying up the rope winding after a few, its binding is fixed on the trunk;
3) grow nonparasitically upon another plant after seedling fixes, will begin in a minute and water, make humidity be controlled at 75%, cultivated through about 2 months time, the seedling of growing nonparasitically upon another plant begins the long shoot that takes root, and seeks connections with on trunk, cultivates through 1 year left and right sides time, and dendrobium candidum is ripe, can gather.Dendrobium candidum need not soil, need not fertile, not medication in whole growth process, mainly is the management of moisture.
For further optimizing such scheme:
The described tree that grows nonparasitically upon another plant is the camphor tree of close grain or cypress, and reason has two: the seeds bark that one of which, texture are thick excessively is aging; The surface overdrying, and be difficult for keeping humidity, not the growth that is beneficial to very much dendrobium candidum; And the seeds bark of close grain can better provide moisture content, and can keep humidity preferably; Its two, the dendrobium candidum root is thinner, is not easy to seek connections with in the thicker bark of texture, and can seeks connections with just in the texture of close grain bark, but do not have the texture seeds, its surface is too smooth, its root can't be sought connections with, and can not grow.Through long term test, wherein, camphor tree or the cypress of trunk diameter between 10-30cm, the growth of optimum dendrobium candidum, but also be not limited thereto.
The said seedling ground clearance H2 that grows nonparasitically upon another plant is 1 meter, and its reason is: in order to prevent the invasion and attack of common damage by disease and insect; The seedling spacing of growing nonparasitically upon another plant H1 is 30-50cm, and its reason is: the height by the dendrobium candidum growth determines that spacing is wide can not to make full use of the trunk resource, and crossing secret meeting influences the dendrobium candidum growth.
Said tie up the rope diameter of section be 1cm; Twining the number of turns is 5 circles; Its reason is: when straw rope can envelope the most of root system of dendrobium candidum; Can pin an amount of moisture again and suitable gas permeability is provided, can satisfy the requirement of dendrobium candidum growth, not so can influence its growth and survival rate moisture and gas permeability.
Twine with tying up rope at said root place at the seedling of growing nonparasitically upon another plant, and its concrete winding position is a coring portion, exposes foundation portion; Its reason is: because foundation portion is the position that the dendrobium candidum sprouting sprouts, the binding position is too high, can cause dendrobium candidum to be difficult for bud and sprout; Influence its growth and survival rate; The binding position is low excessively, can cause dendrobium candidum coring portion can not fully attract to tie up the moisture on the rope, also can influence its growth and survival rate.
The present invention selects annual mid-April to being the field planting time by the end of June, and its reason is: the dendrobium candidum germination period for annual mid-April to June at the bottom of, at this time field planting, the dendrobium candidum survival rate is the highest, growth rate is the fastest.
Through said method tree training dendrobium candidum, its survival rate reaches more than 98%.
Embodiment 2: in embodiment 1, tying up the rope cross-sectional diameter is 2cm, and twining the number of turns is 3 circles, and moisture Control is 70% or 85% o'clock, and dendrobium candidum also can well be grown.
The above is merely preferred embodiment of the present invention, not in order to restriction the present invention, all any modifications of within spirit of the present invention and principle, being done, is equal to and replaces and improvement etc., all is included within protection scope of the present invention.
Claims (8)
1. the tree culture method of a dendrobium candidum is characterized in that this tree culture method comprises the steps:
1) selects the veined seeds in bark surface to be the tree that grows nonparasitically upon another plant, select to be the seedling of growing nonparasitically upon another plant, select straw rope for tying up rope through the dendrobium candidum seedling of taming;
2) seedling of will growing nonparasitically upon another plant places the trunk surface of the tree that grows nonparasitically upon another plant, and a few are fixed with tying up the rope winding at the root place of the seedling of growing nonparasitically upon another plant;
3) tree training humidity is controlled at 70-85%.
2. the tree culture method of a kind of dendrobium candidum according to claim 1 is characterized in that step 2) described in the tree that grows nonparasitically upon another plant be the camphor tree of close grain or cypress.
3. the tree culture method of a kind of dendrobium candidum according to claim 1 is characterized in that step 2) described in liftoff at least 1 meter of the seedling of growing nonparasitically upon another plant, every interval 30-50cm is provided with the circle seedling of growing nonparasitically upon another plant.
4. the tree culture method of a kind of dendrobium candidum according to claim 1 is characterized in that step 2) described in tie up rope to twine the number of turns be the 3-5 circle.
5. the tree culture method of a kind of dendrobium candidum according to claim 1 is characterized in that step 2) described in tie up the rope cross-sectional diameter be 1-2cm.
6. the tree culture method of a kind of dendrobium candidum according to claim 1 is characterized in that step 2) described in twine with tying up rope at the root place of the seedling of growing nonparasitically upon another plant, its concrete winding position is a coring portion, exposes foundation portion.
7. the tree culture method of a kind of dendrobium candidum according to claim 1 is characterized in that selecting annual mid-April to being the field planting time by the end of June.
8. the tree culture method of a kind of dendrobium candidum according to claim 1 is characterized in that the training of tree described in step 3) humidity is controlled at 75%.
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| CN102986509A (en) * | 2012-10-10 | 2013-03-27 | 肥西县农业技术推广中心 | Dendrobium officinale cultivation technique using stone |
| CN103081792A (en) * | 2013-02-01 | 2013-05-08 | 谢金藩 | Dendrobium nobile imitating wild epiphyte cultivation method |
| CN103548652A (en) * | 2013-10-23 | 2014-02-05 | 贵州沿河乌江生物科技发展有限公司 | Rapid tree cultivation method for dendrobium candidum tissue-culture bottle seedlings |
| CN103688832A (en) * | 2013-12-09 | 2014-04-02 | 普洱天磊生物科技有限公司 | Wild imitation planting method of dendrobium officinale |
| CN103718797A (en) * | 2013-12-16 | 2014-04-16 | 莫文油 | High-yield cultivation method for fimbriate dendrobium stems |
| CN103918538A (en) * | 2014-05-04 | 2014-07-16 | 中国热带农业科学院橡胶研究所 | Three-dimensional cultivation method of dendrobium on oil palms |
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| CN102986509A (en) * | 2012-10-10 | 2013-03-27 | 肥西县农业技术推广中心 | Dendrobium officinale cultivation technique using stone |
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| CN103548652A (en) * | 2013-10-23 | 2014-02-05 | 贵州沿河乌江生物科技发展有限公司 | Rapid tree cultivation method for dendrobium candidum tissue-culture bottle seedlings |
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| CN103688832A (en) * | 2013-12-09 | 2014-04-02 | 普洱天磊生物科技有限公司 | Wild imitation planting method of dendrobium officinale |
| CN103688832B (en) * | 2013-12-09 | 2015-11-25 | 普洱天磊生物科技有限公司 | A kind of imitating wild planting process of dendrobium candidum |
| CN103718797A (en) * | 2013-12-16 | 2014-04-16 | 莫文油 | High-yield cultivation method for fimbriate dendrobium stems |
| CN103718797B (en) * | 2013-12-16 | 2015-07-08 | 万潇熠 | High-yield cultivation method for fimbriate dendrobium stems |
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