CN106613831A - Cultivation method of dendrobium officinale with good quality - Google Patents
Cultivation method of dendrobium officinale with good quality Download PDFInfo
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- CN106613831A CN106613831A CN201611012660.7A CN201611012660A CN106613831A CN 106613831 A CN106613831 A CN 106613831A CN 201611012660 A CN201611012660 A CN 201611012660A CN 106613831 A CN106613831 A CN 106613831A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The invention discloses a cultivation method of dendrobium officinale with a good quality. The method comprises the steps of seedbed preparation, seedling breeding, seedling transplanting, and post-transplanting management and harvesting. When the cultivation method is used for cultivating the dendrobium officinale, the output of the dendrobium officinale has good quality, high content of effective components, especially the high content of polysaccharides.
Description
Technical field
The present invention relates to a kind of cultural method of dendrobium candidum, the cultivation of the high dendrobium candidum of particularly a kind of polyoses content
Method.
Background technology
Dendrobium candidum another name iron sheet orchid, bracketplant, Yunnan iron sheet, iron sheet bracketplant, are orchid family Dendrobium herbaceos perennials,
Dendrobium candidum is the top grade of Shihu " medicinal materials, have the laudatory title of " gold in Chinese celestial grass-medicine ", is used as medicine with stem, containing polysaccharide, stone
The active ingredients such as dry measure used in former times alkali, alkaloid, with nourishing Yin and clearing heat, the beneficial stomach that promotes the production of body fluid, wet one's whistle improving eyesight the effects such as, it is antitumor, improve human body
The aspects such as immunity, treatment enterogastric diseases, anti-aging, anti-oxidant, platelet aggregation-against, reduction blood sugar, treatment cataract are
There is good efficacy.
Modern pharmacology research shows that dendrobium candidum has humidification to non-specific and specific immunity, anti-swollen
Knurl, anti-oxidant, the reduction aspect such as blood sugar and cataractous treatment, pharmacological action is notable.Its chemical composition is more complicated, it has been determined that
Type of compounds mainly have：Alkaloids, polysaccharide, luxuriant and rich with fragrance class, bibenzyl parent nucleus class, wherein alkaloids, polysaccharide pharmacology is lived
Property is more notable.Polysaccharide constituents are that medicinal dendrobium plays the important substance basis that immunocompetence is acted on, Dendrobium officinale polysaccharide energy
Peripheral leukocytes are significantly improved, promotes lymphocyte to produce migration inhibition factor, immunity of organisms can be effectively improved.2010 editions
《Pharmacopeia》The quality standard of dendrobium candidum has been recorded first, and assay has been carried out to polysaccharide and mannose.
As the health-care efficacy of dendrobium candidum is widely recognized and received by people, dendrobium officinale far can not expire
The demand of sufficient people.People are had begun to using artificial cultivation dendrobium candidum, and in the cultural method of dendrobium candidum, culture medium is iron
Skin stem of noble dendrobium conditions on which persons or things depend for existence, different culture raw materials and with can be to the growth of dendrobium candidum, yield and active component etc.
Produce important impact.The culture medium of dendrobium candidum and existing cultural method in prior art, it is unfavorable for the iron sheet stone of output
The quality guarantee of dry measure used in former times.Particularly it cannot be guaranteed that in dendrobium candidum polysaccharide content so that polysaccharide in the dendrobium candidum for finally giving
Content is low.
The content of the invention
The purpose of the present invention, is to provide a kind of cultural method of the high dendrobium candidum of polyoses content, the inventive method culture
During dendrobium candidum, the dendrobium candidum quality better of final output, active constituent content is high, particularly the height of the content of polysaccharide.
What the present invention was realized in.
A kind of cultural method of the dendrobium candidum of quality better, including seedbed prepares, seedling breed, seedling replanting, after transplanting
Management and harvesting.
The cultural method of the dendrobium candidum of aforesaid quality better, specifically includes following steps：
(1) seedbed prepares：Width 10-15m, high 4-6m, the booth of long 40-50m is selected to lay in the top of booth 1-1.5m
Sunshade net, from the ground the top laying of 2.5-3.5m is incubated net in booth；
(2) seedling breeds：Dendrobium candidum bud or leaf bud are chosen, in being put into culture medium, sterile propagation culture, seedling is carried out
1-2cm is grown into, is dispensed into blake bottle and is cultivated, 30-45 strain seedling is cultivated in each blake bottle；
(3) transplanting of seedling：Transplanting time is the 4-6 months, and the growth of seedling in blake bottle takes out to during 5～8cm, first with clear
Water cleans the culture medium of seedling root, then with the potassium permanganate dilution immersion root sterilization of 0.3-0.5%, matrix is uniformly filled out to 32
In the hole tray of cave, it is positioned on seedbed, waters, be transplanted in a hole tray from 3～5 plants of compositions one clump of seedling, and matrix is covered
Firmly root system, makes root system contact with matrix；
(4) management after transplanting；
(5) harvest：The stem branch of 3 years raw above is cut, tender stem is left and is allowed its continued growth.
Management after the cultural method step (4) of the dendrobium candidum of aforesaid quality better is transplanted is：
A. water：Using the mode that sprays, irrigation times are 2-3 days 1-3 months 1 time, 1-2 days 3-6 months 1 time, 6-9 months
5-6 days 1 time, 2-3 days 9-12 months 1 time；
B. weeding is topdressed：Weeding is periodically carried out to cultivation base and plays tongue process, 10-15 days after transplanting start to spray potassium nitrate
Or potassium dihydrogen phosphate, sprayed every 5-8 days once, it is every after surviving to spray within 10-15 days a potassium nitrate or potassium dihydrogen phosphate；
C. Temperature and Humidity Control：Ensure that canopy temperature is maintained at 18-22 DEG C daytime, at 15-20 DEG C of night, humid control exists
60%-80%；
D. the prevention and control of plant diseases, pest control：Monthly being sprayed with the thiophanate methyl aqueous solution that concentration is 1-2 ‰ carries out foliar control, insect prevention
Using fly net, can harvesting after 2 years.
During cultural method step (2) seedling of the dendrobium candidum of aforesaid quality better breeds, described culture medium mainly by
Citric acid 1-10 parts, peptone 10-20 parts, potato 50-60 parts, banana 50-60 parts, the agar 20-30 for calculating by weight
Part, hymsleya amabilis 5-15 parts, cymose buckwheat rhizome 10-20 parts, sargentodoxa cuneata 5-15 parts, Japanses beauty-berry 10-20 parts and gunny 1-8 parts are made as follows
It is standby：Hymsleya amabilis, cymose buckwheat rhizome, sargentodoxa cuneata, Japanses beauty-berry and gunny are taken, after being ground into fine grained, plus 8-12 times is measured decocting and boil 2-3h, collected
Decoction liquor, obtains A product；Citric acid and peptone are added at room temperature in A product, stirs complete to dissolving, obtain B product；By potato
Add water after boiling, rub into mashed potato, obtain C product；C product are added in B product, is stirred, obtain D product；Banana adds D product after blending
In, stir, obtain E product；Agar adds at room temperature 3-5 times and measures water, stirs to after being completely dissolved, and obtains F product；F product add E product
In, mix, obtain G product；G product high pressure steam sterilization 20-30 minutes, are obtained final product loaded in tissue culture bottle after cooled and solidified.
Culture medium described in the cultural method of the dendrobium candidum of aforesaid quality better is mainly by the citric acid 5 for calculating by weight
Part, 15 parts of peptone, 55 parts of potato, 55 parts of banana, 25 parts of agar, 10 parts of hymsleya amabilis, 15 parts of cymose buckwheat rhizome, 10 parts of sargentodoxa cuneata, purple
4 parts of 15 parts of pearl and gunny are prepared as follows：Hymsleya amabilis, cymose buckwheat rhizome, sargentodoxa cuneata, Japanses beauty-berry and gunny are taken, thin is ground into
After grain, plus 10 times of amount decoctings boil 2.5h, collect decoction liquor, obtain A product；Citric acid and peptone are added at room temperature in A product, is stirred
Mix complete to dissolving, obtain B product；Potato is added water after boiling, rub into mashed potato, obtain C product；C product, stirring are added in B product
Uniformly, D product are obtained；Banana is added in D product after blending, and is stirred, and obtains E product；Agar adds at room temperature 4 times of amount water, stirs to complete
After CL, F product are obtained；F product are added in E product, are mixed, and obtain G product；G product are loaded in tissue culture bottle, and high pressure steam sterilization 25 minutes is cold
But obtain final product after solidifying.
In the transplanting of cultural method step (3) seedling of the dendrobium candidum of aforesaid quality better, the matrix is mainly by pressing
Peat 50-60 parts of listed as parts by weight, bark residue 30-40 part, wood shavings particle 30-50 parts and Chinese medicine slag 30-40 parts mix.
Matrix described in the cultural method of the dendrobium candidum of aforesaid quality better mainly by 55 parts of the peat for calculating by weight,
35 parts of 35 parts of bark residue, 40 parts of wood shavings particle and Chinese medicine slag are mixed.
Chinese medicine slag described in the cultural method of the dendrobium candidum of aforesaid quality better is mainly by the folium lycii for calculating by weight
1-10 parts, azedarach 1-6 parts, Fructus meliae toosendan 1-4 parts and celastrus orbiculatus 1-12 parts prepare as follows：Take folium lycii, azedarach, river
Chinaberry and celastrus orbiculatus, plus 8-12 times measured decocting and boil 2-3h, decoction liquor is filtered, and takes filter residue, and crushed after being dried is obtained final product into fine grained.
Chinese medicine slag described in the cultural method of the dendrobium candidum of aforesaid quality better is mainly by the folium lycii 5 for calculating by weight
Part, 3 parts of azedarach, 2 parts of Fructus meliae toosendan and 6 parts of celastrus orbiculatus prepare as follows：Take folium lycii, azedarach, Fructus meliae toosendan and southern snake
Rattan, plus 10 times of amount decoctings boil 2.5h, decoction liquor is filtered, and takes filter residue, and crushed after being dried is obtained final product into fine grained.
Applicant has carried out substantial amounts of experimental study to the present invention, and part Experiment is as follows：
The polyoses content of experimental example 1 is investigated.
1 materials and methods
1.1 materials
1.1.1 dendrobium candidum 1：After being cultivated by embodiment 1, harvesting obtains dendrobium candidum 1.
1.1.2 dendrobium candidum 2：During cultivation, hymsleya amabilis, cymose buckwheat rhizome, sargentodoxa cuneata, Japanses beauty-berry and gunny are not contained in culture medium, its
After remaining method is cultivated with embodiment 1, harvesting obtains dendrobium candidum 2.
1.2 instruments and reagent
Dextrose standard sample [glucose of D mono- (+)]：KAYON companies；Methyl alcohol (chromatographically pure)：Close europeanized of Tianjin section
Reagent Co., Ltd；It is pure that remaining reagent is analysis；Phenol：Shanghai Ling Feng chemical reagent Co., Ltd；Sulfuric acid：Insult peaking in Shanghai
Learn reagent Co., Ltd.
2 methods
The preparation of 2.1 need testing solutions
2.1.1 the preparation of need testing solution 1：
Dendrobium candidum of the present invention (dendrobium candidum 1) sample is taken, is put 50 DEG C in baking oven, be dry, pulverize, cross No. three sieves, taken
Medicinal powder after filter, volatilizes solvent, and add water 2000mL, is heated to reflux 2 hours, lets cool, in being transferred to 250mL measuring bottles, with a small quantity
Moisture time washing container, washing lotion is incorporated in same measuring bottle, adds water to scale, is shaken up, and is filtered, and precision measures subsequent filtrate 2mL, puts
In 15mL centrifuge tubes, precision adds 95% ethanol, shakes up, and refrigerates 1 hour, takes out, and is centrifuged 20 minutes, abandoning supernatant, precipitation
Plus hot water dissolving, in being transferred to 25mL measuring bottles, let cool, scale is added water to, shake up, obtain final product need testing solution 1.
2.1.2 the preparation of need testing solution 2
The sample of dendrobium candidum 2 is taken, is put 50 DEG C in baking oven, be dry, pulverize, cross No. three sieves, take medicinal powder after filtration, volatilized
Solvent, add water 2000mL, is heated to reflux 2 hours, lets cool, and in being transferred to 250mL measuring bottles, with a small amount of moisture time washing container, washes
Liquid is incorporated in same measuring bottle, adds water to scale, is shaken up, filtration, and precision measures subsequent filtrate 2mL, and in putting 15mL centrifuge tubes, precision adds
Enter 95% ethanol, shake up, refrigerate 1 hour, take out, be centrifuged 20 minutes, abandoning supernatant, precipitation adds hot water dissolving, is transferred to
In 25mL measuring bottles, let cool, add water to scale, shake up, obtain final product need testing solution 2.
The preparation of 2.2 reference substance solutions
The DEXTROSE ANHYDROUS that precision weighs 105 DEG C of dryings to constant weight is appropriate, adds water and makes 99mg/L solution, standby.
It is prepared by 2.3 calibration curves
Precision measures reference substance solution 0.2,0.4,0.6,0.8,1.0mL and puts in 10mL tool plug test tubes, adds water to 1.0mL,
Precision adds 5% phenol solution 1mL (I is now prepared before use), shakes up, then accurate addition sulfuric acid 5mL, shakes up, in putting boiling water bath
Heating 20 minutes, takes out, and is put into ice-water bath and cools down 5 minutes, with corresponding reagent as blank, determines at 488nm wavelength and inhales
Luminosity, with absorbency Y as ordinate, concentration X (g/L) is abscissa, draws calibration curve, and regression equation is：Y=11.772X-
0.1492, r=0.9995.As a result show that polysaccharide linear relationship in the range of 0.02-0.10g/L is good.
2.4 determination methods
Respectively precision measures need testing solution 1 and each 0.5mL of need testing solution 2, in putting 10mL tool plug test tubes, adds water to
1mL, by the method under the preparation of " 2.3 " calibration curve, from " precision adds 5% phenol solution 1mL ", determines extinction in accordance with the law
Degree, the content of DEXTROSE ANHYDROUS in need testing solution is read from calibration curve, is calculated, and is obtained final product.
2.5 precision tests
Precision draws 6 parts of glucose control product solution, every part of 0.6mL, according to the method mensuration absorbance under " 2.4 " item.
Measurement result, RSD is respectively 1.60% and 1.62%, and precision is good.
2.6 replica tests
6 parts of need testing solutions 1 and need testing solution 2 are prepared as stated above, according to mensuration absorbance as stated above.Determine
As a result, RSD is respectively 2.70% and 2.71%, and repeatability is good.
2.7 stability tests
Take same need testing solution 1 and need testing solution 2 respectively, place at room temperature, respectively at 10min, 30min, 1h,
Mensuration absorbance as stated above after 2h, 4h, 6h.Measurement result, RSD is respectively 2.70% and 2.73%, shows that test sample is molten
Liquid 1 and need testing solution 2 are stable in 6 hours.
2.8 average recoveries are tested
6 parts of 1 powder of dendrobium candidum of known content (12.5%) is weighed, per part of about 1.65g is accurately weighed.Accurately add
Glucose control product solution (concentration is 0.099g/L) 2mL, prepares as stated above need testing solution 1, determines as stated above,
Polyoses content is calculated, as a result average recovery rate is 2.7% for 94.9%, RSD.
6 parts of 2 powder of dendrobium candidum of known content (12.5%) is weighed, per part of about 1.65g is accurately weighed.Accurately add
Glucose control product solution (concentration is 0.099g/L) 2mL, prepares as stated above need testing solution 2, determines as stated above,
Polyoses content is calculated, as a result average recovery rate is 2.6% for 93.9%, RSD.
2.9 sample determinations
By above-mentioned need testing solution 1 and the preparation method and condition determination of need testing solution 2,6 parts of samples of dendrobium candidum 1 are determined
The content of polysaccharide in product and 6 parts of samples of dendrobium candidum 2, records result, and results averaged the results are shown in Table 1.
The determination of polysaccharide result of table 1
As seen from table, the dendrobium candidum that the inventive method cultivation is obtained, the content of polysaccharide in the dendrobium candidum of final harvesting
It is higher.The quality better of dendrobium candidum.
Compared with prior art, during the inventive method culture dendrobium candidum, the dendrobium candidum quality better of final output, effectively
Component content is high, particularly the height of the content of polysaccharide.
Specific embodiment
Embodiment 1.
Culture medium prescription：Citric acid 50kg, peptone 150kg, potato 550kg, banana 550kg, agar 250kg, snow
Courage 100kg, cymose buckwheat rhizome 150kg, sargentodoxa cuneata 100kg, Japanses beauty-berry 150kg and gunny 40kg.
Culture matrix manufacturing：
1st, hymsleya amabilis, cymose buckwheat rhizome, sargentodoxa cuneata, Japanses beauty-berry and gunny are taken, after being ground into fine grained, plus 10 times of amount decoctings are boiled
2.5h, collects decoction liquor, obtains A product；
2nd, citric acid and peptone are added at room temperature in A product, stirs complete to dissolving, obtain B product；
3rd, potato is added water after boiling, rubs into mashed potato, obtain C product；
4th, C product are added in B product, is stirred, obtain D product；
5th, add in D product after banana is blended, stir, obtain E product；
6th, agar adds at room temperature 4 times of amount water, stirs to after being completely dissolved, and obtains F product；
7th, F product are added in E product, are mixed, and obtain G product；
8th, loaded in tissue culture bottle, high pressure steam sterilization 25 minutes is obtained final product G product after cooled and solidified.
It is prepared by Chinese medicine slag：Take folium lycii 50kg, azedarach 30kg, Fructus meliae toosendan 20kg and celastrus orbiculatus 60kg, plus 10 times of amount water
2.5h is decocted, decoction liquor is filtered, and takes filter residue, and crushed after being dried is obtained final product into fine grained.
It is prepared by matrix：Take peat 550kg, bark residue 350kg, wood shavings particle 400kg and Chinese medicine slag 350kg to mix.
The cultural method of dendrobium candidum：
1st, seedbed prepares：Width 12m, high 5m, the booth of long 45m is selected sunshade net to be laid in the top of booth 1.2m, big
From the ground the top laying of 3m is incubated net in canopy；
2nd, seedling breeds：Dendrobium candidum bud or leaf bud are chosen, in being put into culture medium, sterile propagation culture, seedling is carried out
1-2cm is grown into, is dispensed into blake bottle and is cultivated, 30-45 strain seedling is cultivated in each blake bottle；
3rd, the transplanting of seedling：Transplanting time is mid-April, and the growth of seedling in blake bottle takes out to during 5～8cm, first uses
Clear water cleans the culture medium of seedling root, then with 0.4% potassium permanganate dilution immersion root sterilization, matrix is uniformly filled out to 32 caves
In hole tray, it is positioned on seedbed, waters, be transplanted in a hole tray from 3～5 plants of compositions one clump of seedling, and matrix is covered
Root system, makes root system contact with matrix；
4th, the management after transplanting：
A. water：Using the mode that sprays, irrigation times are 1-3 months 2 day 1 time, 3-6 months 2 day 1 time, 6 days 1 6-9 months
It is secondary, 9-12 months 3 day 1 time；
B. weeding is topdressed：Weeding is periodically carried out to cultivation base and plays tongue process, 10-15 days after transplanting start to spray nitric acid
Potassium, sprayed once every 5-8 days, every after surviving to spray within 10-15 days a potassium dihydrogen phosphate；
C. Temperature and Humidity Control：Ensure that canopy temperature is maintained at 18-22 DEG C daytime, at 15-20 DEG C of night, humid control exists
60%-80%；
D. the prevention and control of plant diseases, pest control：Monthly being sprayed with the thiophanate methyl aqueous solution that concentration is 1-2 ‰ carries out foliar control, insect prevention
Using fly net, can harvesting after 2 years；
Harvesting：The stem branch of 3 years raw above is cut, tender stem is left and is allowed its continued growth.
Embodiment 2.
Culture medium prescription：Citric acid 10kg, peptone 20kg, potato 60kg, banana 60kg, agar 30kg, hymsleya amabilis
15kg, cymose buckwheat rhizome 20kg, sargentodoxa cuneata 15kg, Japanses beauty-berry 20kg and gunny 8kg.
Culture matrix manufacturing：
1st, hymsleya amabilis, cymose buckwheat rhizome, sargentodoxa cuneata, Japanses beauty-berry and gunny are taken, after being ground into fine grained, plus 10 times of amount decoctings are boiled
2.5h, collects decoction liquor, obtains A product；
2nd, citric acid and peptone are added at room temperature in A product, stirs complete to dissolving, obtain B product；
3rd, potato is added water after boiling, rubs into mashed potato, obtain C product；
4th, C product are added in B product, is stirred, obtain D product；
5th, add in D product after banana is blended, stir, obtain E product；
6th, agar adds at room temperature 4 times of amount water, stirs to after being completely dissolved, and obtains F product；
7th, F product are added in E product, are mixed, and obtain G product；
8th, loaded in tissue culture bottle, high pressure steam sterilization 25 minutes is obtained final product G product after cooled and solidified.
It is prepared by Chinese medicine slag：Take folium lycii 50kg, azedarach 30kg, Fructus meliae toosendan 20kg and celastrus orbiculatus 60kg, plus 10 times of amount water
2.5h is decocted, decoction liquor is filtered, and takes filter residue, and crushed after being dried is obtained final product into fine grained.
It is prepared by matrix：Take peat 550kg, bark residue 350kg, wood shavings particle 400kg and Chinese medicine slag 350kg to mix.
The cultural method of dendrobium candidum：
1st, seedbed prepares：Width 12m, high 5m, the booth of long 45m is selected sunshade net to be laid in the top of booth 1.2m, big
From the ground the top laying of 3m is incubated net in canopy；
2nd, seedling breeds：Dendrobium candidum bud or leaf bud are chosen, in being put into culture medium, sterile propagation culture, seedling is carried out
1-2cm is grown into, is dispensed into blake bottle and is cultivated, 30-45 strain seedling is cultivated in each blake bottle；
3rd, the transplanting of seedling：Transplanting time is mid-April, and the growth of seedling in blake bottle takes out to during 5～8cm, first uses
Clear water cleans the culture medium of seedling root, then with 0.4% potassium permanganate dilution immersion root sterilization, matrix is uniformly filled out to 32 caves
In hole tray, it is positioned on seedbed, waters, be transplanted in a hole tray from 3～5 plants of compositions one clump of seedling, and matrix is covered
Root system, makes root system contact with matrix；
4th, the management after transplanting：
A. water：Using the mode that sprays, irrigation times are 1-3 months 2 day 1 time, 3-6 months 2 day 1 time, 6 days 1 6-9 months
It is secondary, 9-12 months 3 day 1 time；
B. weeding is topdressed：Weeding is periodically carried out to cultivation base and plays tongue process, 10-15 days after transplanting start to spray nitric acid
Potassium, sprayed once every 5-8 days, every after surviving to spray within 10-15 days a potassium dihydrogen phosphate；
C. Temperature and Humidity Control：Ensure that canopy temperature is maintained at 18-22 DEG C daytime, at 15-20 DEG C of night, humid control exists
60%-80%；
D. the prevention and control of plant diseases, pest control：Monthly being sprayed with the thiophanate methyl aqueous solution that concentration is 1-2 ‰ carries out foliar control, insect prevention
Using fly net, can harvesting after 2 years；
Harvesting：The stem branch of 3 years raw above is cut, tender stem is left and is allowed its continued growth.
Embodiment 3.
Culture medium prescription：Citric acid 1kg, peptone 10kg, potato 50kg, banana 50kg, agar 20kg, hymsleya amabilis 5kg,
Cymose buckwheat rhizome 10kg, sargentodoxa cuneata 5kg, Japanses beauty-berry 10kg and gunny 1kg.
Culture matrix manufacturing：
1st, hymsleya amabilis, cymose buckwheat rhizome, sargentodoxa cuneata, Japanses beauty-berry and gunny are taken, after being ground into fine grained, plus 10 times of amount decoctings are boiled
2.5h, collects decoction liquor, obtains A product；
2nd, citric acid and peptone are added at room temperature in A product, stirs complete to dissolving, obtain B product；
3rd, potato is added water after boiling, rubs into mashed potato, obtain C product；
4th, C product are added in B product, is stirred, obtain D product；
5th, add in D product after banana is blended, stir, obtain E product；
6th, agar adds at room temperature 4 times of amount water, stirs to after being completely dissolved, and obtains F product；
7th, F product are added in E product, are mixed, and obtain G product；
8th, loaded in tissue culture bottle, high pressure steam sterilization 25 minutes is obtained final product G product after cooled and solidified.
It is prepared by Chinese medicine slag：Take folium lycii 50kg, azedarach 30kg, Fructus meliae toosendan 20kg and celastrus orbiculatus 60kg, plus 10 times of amount water
2.5h is decocted, decoction liquor is filtered, and takes filter residue, and crushed after being dried is obtained final product into fine grained.
It is prepared by matrix：Take peat 550kg, bark residue 350kg, wood shavings particle 400kg and Chinese medicine slag 350kg to mix.
The cultural method of dendrobium candidum：
1st, seedbed prepares：Width 12m, high 5m, the booth of long 45m is selected sunshade net to be laid in the top of booth 1.2m, big
From the ground the top laying of 3m is incubated net in canopy；
2nd, seedling breeds：Dendrobium candidum bud or leaf bud are chosen, in being put into culture medium, sterile propagation culture, seedling is carried out
1-2cm is grown into, is dispensed into blake bottle and is cultivated, 30-45 strain seedling is cultivated in each blake bottle；
3rd, the transplanting of seedling：Transplanting time is mid-April, and the growth of seedling in blake bottle takes out to during 5～8cm, first uses
Clear water cleans the culture medium of seedling root, then with 0.4% potassium permanganate dilution immersion root sterilization, matrix is uniformly filled out to 32 caves
In hole tray, it is positioned on seedbed, waters, be transplanted in a hole tray from 3～5 plants of compositions one clump of seedling, and matrix is covered
Root system, makes root system contact with matrix；
4th, the management after transplanting：
A. water：Using the mode that sprays, irrigation times are 1-3 months 2 day 1 time, 3-6 months 2 day 1 time, 6 days 1 6-9 months
It is secondary, 9-12 months 3 day 1 time；
B. weeding is topdressed：Weeding is periodically carried out to cultivation base and plays tongue process, 10-15 days after transplanting start to spray nitric acid
Potassium, sprayed once every 5-8 days, every after surviving to spray within 10-15 days a potassium dihydrogen phosphate；
C. Temperature and Humidity Control：Ensure that canopy temperature is maintained at 18-22 DEG C daytime, at 15-20 DEG C of night, humid control exists
60%-80%；
D. the prevention and control of plant diseases, pest control：Monthly being sprayed with the thiophanate methyl aqueous solution that concentration is 1-2 ‰ carries out foliar control, insect prevention
Using fly net, can harvesting after 2 years；
Harvesting：The stem branch of 3 years raw above is cut, tender stem is left and is allowed its continued growth.
Claims (9)
1. a kind of cultural method of the dendrobium candidum of quality better, it is characterised in that：Breed including seedbed prepares, seedling, seedling moves
Management and harvesting after cultivation, transplanting.
2. the cultural method of the dendrobium candidum of quality better as claimed in claim 1, it is characterised in that：Specifically include following step
Suddenly：
（1）Seedbed prepares：Width 10-15m, high 4-6m, the booth of long 40-50m is selected to lay sunshade in the top of booth 1-1.5m
Net, from the ground the top laying of 2.5-3.5m is incubated net in booth；
（2）Seedling breeds：Dendrobium candidum bud or leaf bud are chosen, in being put into culture medium, sterile propagation culture, growth of seedling is carried out
To 1-2cm, it is dispensed into blake bottle and is cultivated, 30-45 strain seedling is cultivated in each blake bottle；
（3）The transplanting of seedling：Transplanting time is the 4-6 months, and the growth of seedling in blake bottle takes out to during 5～8cm, first washed with clear water
The culture medium of net seedling root, then with the potassium permanganate dilution immersion root sterilization of 0.3-0.5%, matrix is uniformly filled out to 32 cave caves
In disk, it is positioned on seedbed, waters, be transplanted in a hole tray from 3～5 plants of compositions one clump of seedling, and matrix is covered into root
System, makes root system contact with matrix；
（4）Management after transplanting；
（5）Harvesting：The stem branch of 3 years raw above is cut, tender stem is left and is allowed its continued growth.
3. the cultural method of the high dendrobium candidum of polyoses content as claimed in claim 2, it is characterised in that：Step（4）Transplant
Management afterwards is：
A. water：Using the mode that sprays, irrigation times are 2-3 days 1-3 months 1 time, 1-2 days 3-6 months 1 time, 6-9 months 5-6
It 1 time, 2-3 days 9-12 months 1 time；
B. weeding is topdressed：Weeding is periodically carried out to cultivation base and plays tongue process, start within 10-15 days after transplanting to spray potassium nitrate or phosphorus
Acid dihydride potassium, sprayed once every 5-8 days, every after surviving to spray within 10-15 days a potassium nitrate or potassium dihydrogen phosphate；
C. Temperature and Humidity Control：Ensure that canopy temperature is maintained at 18-22 DEG C daytime, at 15-20 DEG C of night, humid control is in 60%-
80%；
D. the prevention and control of plant diseases, pest control：Monthly being sprayed with the thiophanate methyl aqueous solution that concentration is 1-2 ‰ carries out foliar control, and insect prevention is adopted
Fly net, can harvesting after 2 years.
4. the cultural method of the dendrobium candidum of the quality better described in claim 2, it is characterised in that：Step（2）During seedling breeds,
Described culture medium is mainly by citric acid 1-10 parts, peptone 10-20 parts, potato 50-60 parts, the banana for calculating by weight
50-60 parts, agar 20-30 parts, hymsleya amabilis 5-15 parts, cymose buckwheat rhizome 10-20 parts, sargentodoxa cuneata 5-15 parts, Japanses beauty-berry 10-20 parts and gunny
1-8 parts prepare as follows：Hymsleya amabilis, cymose buckwheat rhizome, sargentodoxa cuneata, Japanses beauty-berry and gunny are taken, after being ground into fine grained, plus 8-12 times
Amount decocting boils 2-3h, collects decoction liquor, obtains A product；Citric acid and peptone are added at room temperature in A product, is stirred to having dissolved
Entirely, B product are obtained；Potato is added water after boiling, rub into mashed potato, obtain C product；C product are added in B product, is stirred, obtain D product；
Banana is added in D product after blending, and is stirred, and obtains E product；Agar adds at room temperature 3-5 times and measures water, stirs to after being completely dissolved,
Obtain F product；F product are added in E product, are mixed, and obtain G product；G product are loaded in tissue culture bottle, high pressure steam sterilization 20-30 minutes, cooled and solidified
After obtain final product.
5. the cultural method of the dendrobium candidum of quality better as claimed in claim 4, it is characterised in that：The culture medium mainly by
5 parts of the citric acid for calculating by weight, 15 parts of peptone, 55 parts of potato, 55 parts of banana, 25 parts of agar, 10 parts of hymsleya amabilis, Jin Qiao
4 parts of 15 parts of wheat, 10 parts of sargentodoxa cuneata, 15 parts of Japanses beauty-berry and gunny are prepared as follows：Take hymsleya amabilis, cymose buckwheat rhizome, sargentodoxa cuneata, Japanses beauty-berry
And gunny, after being ground into fine grained, plus 10 times of amount decoctings boil 2.5h, collect decoction liquor, obtain A product；By citric acid and peptone
Add in A product at room temperature, stir complete to dissolving, obtain B product；Potato is added water after boiling, rub into mashed potato, obtain C
Product；C product are added in B product, is stirred, obtain D product；Banana is added in D product after blending, and is stirred, and obtains E product；Agar is in room temperature
Down plus 4 times amount water, stir to after being completely dissolved, obtain F product；F product are added in E product, are mixed, and obtain G product；G product are high loaded in tissue culture bottle
Pressure steam sterilizing 25 minutes, obtains final product after cooled and solidified.
6. the cultural method of the dendrobium candidum of quality better as claimed in claim 2, it is characterised in that：Step（3）The shifting of seedling
In cultivation, the matrix mainly by the peat 50-60 part, bark residue 30-40 part, wood shavings particle 30-50 parts for calculating by weight and
Chinese medicine slag 30-40 parts mix.
7. the cultural method of the dendrobium candidum of quality better as claimed in claim 5, it is characterised in that：The matrix is mainly by pressing
35 parts of 55 parts of the peat of listed as parts by weight, 35 parts of bark residue, 40 parts of wood shavings particle and Chinese medicine slag are mixed.
8. the cultural method of the dendrobium candidum of quality better as claimed in claims 6 or 7, it is characterised in that：The Chinese medicine slag master
Will be by folium lycii 1-10 parts, azedarach 1-6 parts, Fructus meliae toosendan 1-4 parts and the celastrus orbiculatus 1-12 part for calculating by weight by following sides
It is prepared by method：Folium lycii, azedarach, Fructus meliae toosendan and celastrus orbiculatus are taken, plus 8-12 times is measured decocting and boils 2-3h, decoction liquor is filtered, and takes filter residue,
Crushed after being dried is obtained final product into fine grained.
9. the cultural method of dendrobium candidum as claimed in claim 8, it is characterised in that：The Chinese medicine slag is mainly by by weight
6 parts of 5 parts of the folium lycii of calculating, 3 parts of azedarach, 2 parts of Fructus meliae toosendan and celastrus orbiculatus prepare as follows：Take folium lycii, azedarach,
Fructus meliae toosendan and celastrus orbiculatus, plus 10 times of amount decoctings boil 2.5h, decoction liquor is filtered, and takes filter residue, and crushed after being dried is obtained final product into fine grained.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107027606A (en) * | 2017-05-17 | 2017-08-11 | 贵州济生农业科技有限公司 | A kind of cultural method of the high dendrobium candidum of stem of noble dendrobium alkali content |
| CN107114223A (en) * | 2017-05-12 | 2017-09-01 | 贵州济生农业科技有限公司 | A kind of cultural method of dendrobium candidum |
| CN107567971A (en) * | 2017-10-16 | 2018-01-12 | 贵州济生农业科技有限公司 | A kind of pseudo-wild cultivating method of dendrobium candidum |
| CN107616085A (en) * | 2017-10-23 | 2018-01-23 | 贵州济生农业科技有限公司 | A kind of pseudo-wild cultivating method of the high dendrobium candidum of polyoses content |
| CN109006435A (en) * | 2018-08-29 | 2018-12-18 | 靖西市金峰贸易有限公司 | A kind of implantation methods of dendrobium candidum |
| CN109089767A (en) * | 2018-08-29 | 2018-12-28 | 靖西市金峰贸易有限公司 | A kind of method of greenhouse gardening dendrobium candidum |
| CN109122080A (en) * | 2018-09-05 | 2019-01-04 | 湖南中新创生物科技有限公司 | A method of improving dendrobium candidum yield and quality |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107114223A (en) * | 2017-05-12 | 2017-09-01 | 贵州济生农业科技有限公司 | A kind of cultural method of dendrobium candidum |
| CN107027606A (en) * | 2017-05-17 | 2017-08-11 | 贵州济生农业科技有限公司 | A kind of cultural method of the high dendrobium candidum of stem of noble dendrobium alkali content |
| CN107567971A (en) * | 2017-10-16 | 2018-01-12 | 贵州济生农业科技有限公司 | A kind of pseudo-wild cultivating method of dendrobium candidum |
| CN107616085A (en) * | 2017-10-23 | 2018-01-23 | 贵州济生农业科技有限公司 | A kind of pseudo-wild cultivating method of the high dendrobium candidum of polyoses content |
| CN109006435A (en) * | 2018-08-29 | 2018-12-18 | 靖西市金峰贸易有限公司 | A kind of implantation methods of dendrobium candidum |
| CN109089767A (en) * | 2018-08-29 | 2018-12-28 | 靖西市金峰贸易有限公司 | A kind of method of greenhouse gardening dendrobium candidum |
| CN109122080A (en) * | 2018-09-05 | 2019-01-04 | 湖南中新创生物科技有限公司 | A method of improving dendrobium candidum yield and quality |
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