CN104016752A - Nutrient solution for increasing yield of pfaffia paniculata roots and application thereof - Google Patents
Nutrient solution for increasing yield of pfaffia paniculata roots and application thereof Download PDFInfo
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- CN104016752A CN104016752A CN201410254010.8A CN201410254010A CN104016752A CN 104016752 A CN104016752 A CN 104016752A CN 201410254010 A CN201410254010 A CN 201410254010A CN 104016752 A CN104016752 A CN 104016752A
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Abstract
The invention relates to the field of plant cultivation, and particularly relates to a nutrient solution for increasing the yield of pfaffia paniculata roots and an application thereof. The nutrient solution for increasing the yield of pfaffia paniculata roots mainly comprises the following components: 300mg/L-500mg/L of sodium selenite, 30mg/L-50mg/L of chitosan, 250 mg/L-400 mg/L of sodium bisulfite and water serving as a solvent. The nutrient solution for increasing the yield of the pfaffia paniculata roots is simple in a preparation manner, has no toxic or side effects and can be used for obviously increasing the yield of the pfaffia paniculata roots. When the nutrient solution is for spraying pfaffia paniculata so as to increase the yield of the pfaffia paniculata roots, the content of the main component saponin in the nutrient solution is stable.
Description
Technical field
The present invention relates to plant growing field, particularly a kind of for improving nutritive medium and the application thereof of pfaffia root output.
Background technology
Pfaffia (Pfaffia paniculata) also claims enamel luxuriant and rich with fragrance sub-(Pfaffia), for Amaranthaceae (Amaranthaceae) Achyranthes per nnial herb, with root, is used as medicine, and has the effects such as clearing heat and detoxicating, powerful establishing-Yang, relieving inflammation and relaxing pain.Be mainly used in treating section's diseases such as cardiovascular, nervus centralis, reproduction, Digestive tract.The luxuriant and rich with fragrance sub-tropical rain forest area that originates in the states such as South America Brazil of enamel.China has introduced a fine variety at present and has succeeded.The luxuriant and rich with fragrance subgenus sun plant of enamel, is grown in the place that sunlight is comparatively sufficient conventionally.Plant height 150cm-200cm, the common 3-5 bar of root, cylindrical, be yellow.Cane is comprised of some joints, and internode is longer, hollow, and the stem of joint is knee shape expands, and side shoot is to life.The luxuriant and rich with fragrance sub-growth of enamel is unbalanced, and nutritive substance distributes inharmonious, and economic coefficient is low.At vegetative growth phase, the luxuriant and rich with fragrance sub-aerial growth of enamel is rapid, and plant is tall and big; Underground part hypoevolutism, the growth of root is significantly limited, and root/shoot ratio is seriously lacked of proper care (up to 1:12), particularly the prosperous length of over-ground part stem, expended a large amount of nutritive substances, blade usually comes off in a large number, and plant is fragile, meets wind and easily lodges, seriously restricted the development in enamel luxuriant and rich with fragrance sub-photosynthetic " source ", greatly reduce the quantity in " source ", added the nutrient competition in strong " storehouse " of stem, very large on the luxuriant and rich with fragrance sub-root yield impact of enamel.
The inventor has carried out a large amount of research and experiments for the problems referred to above in the process of carrying out pfaffia cultivation, it is unexpected that discovery adopt a kind of nutritive medium of human configuration to spray in the specified phase of pfaffia seedling early growth the generation that plant has not only avoided the problems referred to above, but also improved the output of pfaffia medicinal material.
Summary of the invention
The invention provides a kind ofly for improving the nutritive medium of pfaffia root output, this nutrient solution mode is simple, no side effects, and can significantly improve the output of pfaffia root.
The present invention also provides a kind of preparation method of described nutritive medium.
The present invention also provides a kind of method that improves pfaffia root output.
The technical solution adopted for the present invention to solve the technical problems is:
For improving a nutritive medium for pfaffia root output, described nutritive medium mainly consists of the following composition: Sodium Selenite 300mg/L～500mg/L, chitosan 30mg/L～50mg/L, sodium bisulfite 250mg/L～400mg/L, solvent is water.This nutrient solution mode is simple, no side effects, and can significantly improve the output of pfaffia root.
Described nutritive medium consists of the following composition: Sodium Selenite 350mg/L～450mg/L, chitosan 35mg/L～45mg/L, sodium bisulfite 300mg/L～380mg/L, solvent is water.
Described nutritive medium consists of the following composition: Sodium Selenite 400mg/L, chitosan 45mg/L, sodium bisulfite 350mg/L, solvent is water.
A kind of preparation method of described nutritive medium, the method is specially: weigh respectively sodium bisulfite, Sodium Selenite and chitosan, add three kinds of compositions successively in water, stir while adding dissolving, the addition sequence of three kinds of compositions is sodium bisulfite, Sodium Selenite, chitosan, last constant volume.
The application of a kind of described nutritive medium for improving pfaffia root output.
Improve a method for pfaffia root output, with nutritive medium of the present invention, between April to July, pfaffia plant is evenly sprayed, spray frequency and be 4～20 days once.
Further, with described nutritive medium, the front on the blade face of pfaffia plant and/or the back side, front and/or the back side and root are sprayed.
The invention has the beneficial effects as follows: nutritive medium of the present invention can significantly promote the growth of pfaffia root and improve the output of medicinal material.When using nutritive medium of the present invention to spray pfaffia volume increase, its main component saponin content is stable.
Embodiment
Below by specific embodiment, technical scheme of the present invention is described in further detail.Should be appreciated that enforcement of the present invention is not limited to the following examples, any pro forma accommodation that the present invention is made and/or change all will fall into protection domain of the present invention.
In the present invention, if not refer in particular to, all part, per-cents are weight unit, and the equipment adopting and raw material etc. all can be buied from market or this area is conventional.Method in following embodiment, if no special instructions, is the ordinary method of this area.
What " mg/L " in the present invention was unit " mg/litre " writes a Chinese character in simplified form, and is interpreted as: preparing complete volume is the weight that contains the materials such as Sodium Selenite in one liter of solution; " liter " take the graduated cylinder that volume is 1 liter is standard capacity; What " mg " was unit " milligram " writes a Chinese character in simplified form, the weight of the materials such as Sodium Selenite that weigh with applying electronic balance.
Sodium bisulfite: molecular formula NaHSO
3, molecular weight 104.06, purity>=99.5%;
Sodium Selenite: molecular formula Na
2seO
3, molecular weight 172.94, purity>=98%
Chitosan: claim again chitosan, molecular formula: C
56h
103n
9o
39, molecular weight: 1526.4539, purity>=99%.
Experimental example
Experimental example 1 contrast paclobutrazol, Sodium Selenite, chitosan, sodium bisulfite are used separately the effect that pfaffia is promoted to root growth
Paclobutrazol working concentration in the operation of this experimental example and mode according to for < < paclobutrazol, grown in the luxuriant and rich with fragrance Asia of enamel and root yield affect > >, Ling Zhengzhu etc., time precious traditional Chinese medical science traditional Chinese medicines the 21st the 4th phase of volume in 2010.
1, cuttage is processed
Mid-March selected annual pfaffia stalwartness plant, cut middle part stem section and do cutting, in greenhouse, the cuttage of husky bed, treats that cuttage seeding grows 6 leaves, is transplanted to sample plot by seedling.Transplant first 1～2 day in conjunction with ploughing deeply, use base fertilizer.Test adopts randomized block design, seeding row spacing 40cm * 40cm, every district 4m
2, every district field planting 36 strains.Mid-May, pfaffia starts to enter prosperous long-term, then according to requirement of experiment, pfaffia is carried out to front, blade face and evenly sprays different types of nutritive medium, until drip in blade face, within every four days, sprays once.Then, in October, measure the big and heavy of root.
2, nutritive medium kind and concentration setting thereof
Paclobutrazol: be the experiment product of commercially available purity >=98%; Sodium Selenite: be the experiment product of commercially available purity >=98%; Chitosan: be the experiment product of commercially available purity >=99%; Sodium bisulfite is the experiment product of commercially available purity >=99.5%.
This experiment selects four kinds of additives to be mixed with separately respectively front and the back side that nutritive medium sprays pfaffia leaf, within every four days, sprays once, and the concentration that each additive arranges is as shown in table 1.Meanwhile, take the tap water that does not add any additive is contrast (CK).At the beginning of 10 months, experiment is taken off and washed with the root of plant, then measure respectively fresh weight, then calculate the weight in average of every strain pfaffia root.
The preparation of each nutritive medium:
Compound concentration is the paclobutrazol solution of 300mg/L: by electronic balance accurate measuring 300mg paclobutrazol, in 1L beaker, add about 500ml tap water or pure water, then the 300mg paclobutrazol measuring is added in beaker, stir while adding until all dissolvings are finally all poured this solution in the graduated cylinder that volume is 1L standby after constant volume again.
All the other each solution of additive manner of formulation are identical with the paclobutrazol solution allocation mode of above-mentioned 300mg/L, join and use.
By result in table 1, can be found out, with each additive, spraying behind the blade face of pfaffia separately, root heavily be there is no to obvious promoter action, otherwise, when paclobutrazol concentration reaches 500mg/L, concentration of sodium selenite and reaches 500mg/L, chitosan concentration and reach 50mg/L, the fresh weight of pfaffia root is suppressed on the contrary.Do not find that paclobutrazol plays obvious promoter action to pfaffia root growth when 300mg/L, this < < paclobutrazol of delivering from Ling Zhengzhu etc. grown in the luxuriant and rich with fragrance Asia of enamel and root yield to affect > > research different.In a word, in the applicant's research, find, the additive adding separately in table 1 can not promote pfaffia root growth substantially, does not increase the output of pfaffia medicinal material.
Table 1 impact of additive on pfaffia root growth separately
Experimental example 2 contrast paclobutrazol, Sodium Selenite, chitosan, sodium bisulfite combination promote the effect of root growth to pfaffia
This experimental study is the impact on pfaffia root growth for the array mode of additive, and wherein, kind and the concentration thereof of additive are as shown in table 2.Cuttage seeding, spray mode and number of times, experiment strain number, weight determination and calculate all identical with experimental example 1.
The solution preparation mode that contains additive:
1,2,4 the solution preparation mode of being numbered is without singularity, only need to electronic balance, weigh respectively aequum additive according to concentration requirement, then each additive is taked the mode stirring while adding to pour in the beaker that fills pure water or tap water, until all dissolve, finally this solution is all poured in the graduated cylinder that volume is 1L more standby after constant volume.
Be numbered 3,5,6 solution preparation mode, take and number 3 solution as example: first with electronic balance, take the paclobutrazol of 300mg/L, the mode then paclobutrazol being stirred while adding is poured in the beaker that fills pure water or tap water, until all dissolve; Take again the chitosan of 30mg, stir while adding and add in this solution, until all dissolve, finally this solution is all poured in the graduated cylinder that volume is 1L more standby after constant volume.All the other solution that contain shell sucrose composition all need be according to the mode obtain solution of numbering 3.
By result in table 2, can be found out, using two kinds of additive combination solutions to spray behind the blade face of pfaffia, root heavily be there is no to obvious promoter action.
The impact of two kinds of additive combinations of table 2 on pfaffia root growth
Experimental example 3 contrast paclobutrazol, Sodium Selenite, chitosan, three kinds of composition combinations of sodium bisulfite promote the effect of root growth to pfaffia
Experiment purpose and method: this experimental study is the impact on pfaffia root growth for the array mode of additive, and wherein, kind and the concentration thereof of additive are as shown in table 3.Cuttage seeding, spray mode and number of times, experiment strain number, weight determination and calculate all identical with experimental example 1.
The solution preparation mode that contains additive: be numbered in 2,3,4 solution and all contain chitosan, when obtain solution, chitosan need add after all the other additives dissolve again as shown in experimental example 2, finally uses graduated cylinder constant volume standby.
Data as shown in Table 3 can find out, these four kinds of additives are mixed with solution and spray after pfaffia leaf after every three kinds of combinations, only have the solution of numbering 4 to have the effect that obvious promotion pfaffia root fresh weight increases, and the weightening finish of all the other combination roots is not obvious.
The impact of three kinds of additive combinations of table 3 on pfaffia root growth
The additive combination of experimental example 4 different concns is on the impact on pfaffia root growth
Experiment purpose and method: this experimental study is the impact on pfaffia root growth for the concentration of additive, and wherein, kind and the concentration thereof of additive are as shown in table 4.Cuttage seeding, spray mode and number of times, experiment strain number, weight determination and calculate all identical with experimental example 1.
The solution preparation mode that contains additive: except CK, all contain chitosan in solution, when obtain solution, chitosan need add after all the other additives dissolve again as shown in experimental example 2, finally uses graduated cylinder constant volume standby.
As can be seen from Table 4: three kinds of additive compositions are respectively within the scope of finite concentration and be used in conjunction with and just can play the effect that promotes the weightening finish of pfaffia root and improve output, for example, when concentration of sodium selenite reaches 550mg/L, suppressed on the contrary the weight of pfaffia root; When the concentration of chitosan reaches 60mg/L, this combination nutrient liquid is not remarkable to the weightening finish of pfaffia root.
The additive combination of table 4 different concns is on the impact on pfaffia root growth
Embodiment
Embodiment 1
Mid-March selected annual pfaffia stalwartness plant, cut middle part stem section and do cutting, in greenhouse, the cuttage of husky bed, treats that cuttage seeding grows 6 leaves, is transplanted to sample plot by seedling.Transplant first 1～2 day in conjunction with ploughing deeply, use base fertilizer.Test adopts randomized block design, seeding row spacing 40cm * 40cm, every district 12m
2, every district field planting 100 strains.Mid-June, pfaffia is carried out to the even spray nutritious liquor of blade face pro and con, wherein, the composition of nutritive medium is: Sodium Selenite 300mg/L, sodium bisulfite 400mg/L, chitosan 45mg/L, surplus are water.Identical described in experimental example of the manner of formulation of this nutritive medium.Until drip in blade face, within every 8 days, spray once.Then, the counterpoise of measuring the big and heavy of root and calculating every strain pfaffia root in October is 63.36mg.
Embodiment 2
Mid-March selected annual pfaffia stalwartness plant, cut middle part stem section and do cutting, in greenhouse, the cuttage of husky bed, treats that cuttage seeding grows 6 leaves, is transplanted to sample plot by seedling.Transplant first 1～2 day in conjunction with ploughing deeply, use base fertilizer.Test adopts randomized block design, seeding row spacing 40cm * 40cm, every district 12m
2, every district field planting 100 strains.Mid-April, pfaffia is carried out to blade face pros and cons and root cultivation matrix surface uniform spray nutritious liquor, the composition of nutritive medium is: Sodium Selenite 500mg/L, sodium bisulfite 250mg/L, chitosan 30mg/L, surplus are water.Identical described in experimental example of the manner of formulation of this nutritive medium.Until drip in blade face, within every 12 days, spray once.Then, the counterpoise of measuring the big and heavy of root and calculating every strain pfaffia root in October is 62.35mg.
Embodiment 3
Mid-March selected annual pfaffia stalwartness plant, cut middle part stem section and do cutting, in greenhouse, the cuttage of husky bed, treats that cuttage seeding grows 6 leaves, is transplanted to sample plot by seedling.Transplant first 1～2 day in conjunction with ploughing deeply, use base fertilizer.Test adopts randomized block design, seeding row spacing 40cm * 40cm, every district 12m
2, every district field planting 100 strains.Mid-April, pfaffia is carried out to blade face reverse side and root cultivation matrix surface uniform spray nutritious liquor, the composition of nutritive medium is: Sodium Selenite 350mg/L, sodium bisulfite 380mg/L, chitosan 45mg/L, surplus are water.Identical described in experimental example of the manner of formulation of this nutritive medium.Until drip in blade face, within every 20 days, spray once.Then, in October, measure the big and heavy of root and calculate the counterpoise 62.13mg of every strain pfaffia root.
Embodiment 4
Mid-March selected annual pfaffia stalwartness plant, cut middle part stem section and do cutting, in greenhouse, the cuttage of husky bed, treats that cuttage seeding grows 6 leaves, is transplanted to sample plot by seedling.Transplant first 1～2 day in conjunction with ploughing deeply, use base fertilizer.Test adopts randomized block design, seeding row spacing 40cm * 40cm, every district 12m
2, every district field planting 100 strains.Mid-April, pfaffia is just being carried out to blade face and root cultivation matrix surface uniform spray nutritious liquor, the composition of nutritive medium is: Sodium Selenite 450mg/L, sodium bisulfite 300mg/L, chitosan 35mg/L, surplus are water.Identical described in experimental example of the manner of formulation of this nutritive medium.Until drip in blade face, within every 20 days, spray once.Then, the counterpoise of measuring the big and heavy of root and calculating every strain pfaffia root in October is 65.53mg.
Embodiment 5
Mid-March selected annual pfaffia stalwartness plant, cut middle part stem section and do cutting, in greenhouse, the cuttage of husky bed, treats that cuttage seeding grows 6 leaves, is transplanted to sample plot by seedling.Transplant first 1～2 day in conjunction with ploughing deeply, use base fertilizer.Test adopts randomized block design, seeding row spacing 40cm * 40cm, every district 12m
2, every district field planting 100 strains.Mid-April, only pfaffia is carried out to the positive evenly spray nutritious liquor in blade face, the composition of nutritive medium is: Sodium Selenite 400mg/L, sodium bisulfite 350mg/L, chitosan 45mg/L, surplus are water.Identical described in experimental example of the manner of formulation of this nutritive medium.Until drip in blade face, within every 4 days, spray once.Then, in October, measure the big and heavy of root and calculate the counterpoise 67.55mg of every strain pfaffia root.
Embodiment 6
Choose at random not standby after the pfaffia root in spray nutritious liquor processing and embodiment 5 is dry.
The preparation of 1 solution
Standardized solution is prepared accurately weighed Oleanolic Acid reference substance, adds the solution that methyl alcohol is mixed with 0.29mg/ml, in contrast product solution.
The preparation of need testing solution:
Precision takes the powder 2g that is not dried constant weight through the pfaffia root in spray nutritious liquor processing and embodiment 5, in 250ml flask, add 80% ethanol 50ml, microwave extraction, filter, get subsequent filtrate 25ml, after water bath method, add water 10ml and dissolve, with the n-butanol extraction of water saturation 3 times, 15ml/ time, merge butanol extraction liquid, use again the water washing of the saturated mistake of 15ml propyl carbinol, collect propyl carbinol liquid, fling to after propyl carbinol with dissolve with methanol, put in 50ml volumetric flask, and by methanol constant volume to scale.
2 measure the selection of wavelength
Get the Oleanolic Acid contrast liquid of 0.5ml, in water-bath, be evaporated to dryly, add 5% Vanillin-Glacial acetic acid 0.2ml of new preparation, perchloric acid 0.8ml, close plug, after 70 ℃ of water-baths colour developing 15min, flowing water is cooled to room temperature.Add vinyl acetic monomer 5ml, shake up, scan immediately at ultraviolet spectrophotometer under 400～800nm wavelength, blank solution is made reference simultaneously, at 542nm place, has maximum absorption, determines that thus detecting wavelength is 542nm.
3 linear relationships are investigated
The above-mentioned reference substance solution 0.00,0.10,0.20,0.30 of accurate absorption, 0.40,0.50ml, water-bath is evaporated to dry, adds 5% Vanillin-Glacial acetic acid (facing with newly joining) 0.2ml, perchloric acid 0.8ml, after 70 ℃ of water-baths colour developing 15min, puts and in flowing water, is cooled to room temperature.Add vinyl acetic monomer 5ml, shake up, blank test is done in retinue, measures absorbancy at 542nm place.Calculating regression equation is: A=6.1775C-0.0089, r=0.9990 is good linear relationship within the scope of 0.0058～0.029mg/ml.
4 stability tests
Accurate Oleanolic Acid reference substance solution (0.29mg/ml) 0.2ml that draws is in tool plug test tube, and by calibration curve method colour developing, retinue reagent is done blank, in the timing of 542nm wavelength place, measures, and every 5min, measures 1 absorbance, measures altogether 30min.After reference substance solution colour developing, absorbance is stable in 5～30min, and RSD=0.068%, therefore colorimetric estimation completes impact not quite in 30min.
The accurate Oleanolic Acid contrast liquid 0.20ml that draws of 5 Precision Experiments, by " 6.3 " lower operation 5 times, measures absorbancy.Calculating RSD is 0.051%.
6 samples contgs are measured
Precision measures sample solution 0.4ml, by " 4 stability test " lower operation, carries out assay.
7 results and analysis
The pfaffia root saponin content that sprays processing without overnutrition liquid is that the pfaffia root saponin content that sprays processing through nutritive medium in 6.78%, embodiment 5 is 6.81%.
From the above results, when using nutritive medium of the present invention to spray pfaffia volume increase, its main component saponin content is stable.
Above-described embodiment is a kind of preferably scheme of the present invention, not the present invention is done to any pro forma restriction, also has other variant and remodeling under the prerequisite that does not exceed the technical scheme that claim records.
Claims (7)
1. one kind for improving the nutritive medium of pfaffia root output, it is characterized in that described nutritive medium mainly consists of the following composition: Sodium Selenite 300mg/L～500mg/L, chitosan 30mg/L～50mg/L, sodium bisulfite 250mg/L～400mg/L, solvent is water.
2. nutritive medium according to claim 1, is characterized in that described nutritive medium consists of the following composition: Sodium Selenite 350mg/L～450mg/L, chitosan 35mg/L～45mg/L, sodium bisulfite 300mg/L～380mg/L, solvent is water.
3. nutritive medium according to claim 1, is characterized in that described nutritive medium consists of the following composition: Sodium Selenite 400mg/L, chitosan 45mg/L, sodium bisulfite 350 mg/L, solvent is water.
4. the preparation method of a nutritive medium claimed in claim 1, it is characterized in that the method is specially: weigh respectively sodium bisulfite, Sodium Selenite and chitosan, in water, add three kinds of compositions successively, stir while adding dissolving, the addition sequence of three kinds of compositions is sodium bisulfite, Sodium Selenite, chitosan, last constant volume.
5. a nutritive medium claimed in claim 1 is used for the application that improves pfaffia root output.
6. a method that improves pfaffia root output, is characterized in that: with nutritive medium claimed in claim 1, between April to July, pfaffia plant is evenly sprayed, spray frequency and be 4～20 days once.
7. the method for raising pfaffia root output according to claim 6, is characterized in that: with described nutritive medium, the front on the blade face of pfaffia plant and/or the back side, front and/or the back side and root are sprayed.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111848302A (en) * | 2019-12-17 | 2020-10-30 | 中国农业大学 | Application of chitosan functionalized nano-selenium composite bacterium enzyme system in improvement of yield and quality of Chinese herbal medicine |
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