CN107616085A - A kind of pseudo-wild cultivating method of the high dendrobium candidum of polyoses content - Google Patents
A kind of pseudo-wild cultivating method of the high dendrobium candidum of polyoses content Download PDFInfo
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Abstract
The invention discloses a kind of pseudo-wild cultivating method of the high dendrobium candidum of polyoses content, once cultivate；Second incubation；Tame and docile seedling；Plantation：Select the 1600m of height above sea level 800, relative humidity 55% 70%, the 1500mm of annual rainfall 1000, more than 10 DEG C of winter temperature, 18 30 DEG C of season of growth temperature, 15 20 DEG C of rest period temperature, the day and night temperature difference keep 10 15 DEG C of tor and the excessive geomorphic province in mud mountain；In March~April, temperature reaches 20 DEG C~25 DEG C, and relative air humidity is 75%~85%, dendrobium candidum plant is divided into one clump by 3 plants~5 plants, root is wrapped with moss, is then put into the gap between stone recessed joint or stone；Top dressing；Watering；Harvesting.During the inventive method cultivation of dendrobium officinale, the dendrobium candidum quality better of final output, active constituent content is high, particularly the height of the content of polysaccharide.
Description
Technical field
The present invention relates to a kind of pseudo-wild cultivating method of dendrobium candidum, the high dendrobium candidum of particularly a kind of polyoses content
Pseudo-wild cultivating method.
Background technology
Dendrobium candidum nickname iron sheet orchid, bracketplant, Yunnan iron sheet, iron sheet bracketplant, it is orchid family Dendrobium herbaceos perennial,
Dendrobium candidum is the top grade of Shihu " medicinal materials, is known as the laudatory title of " gold in Chinese celestial grass-medicine ", is used as medicine with stem, contains polysaccharide, stone
The active ingredients such as dry measure used in former times alkali, alkaloid, with nourishing Yin and clearing heat, the beneficial stomach that promotes the production of body fluid, wet one's whistle improving eyesight and other effects, in antitumor, raising human body
Immunity, treatment enterogastric diseases, anti-aging, anti-oxidant, platelet aggregation-against, reduction blood glucose, treatment cataract etc. are
There is good efficacy.
Modern pharmacology research shows that dendrobium candidum has humidification to non-specific and specific immunity, anti-swollen
Knurl, treatment that is anti-oxidant, reducing blood glucose and cataract etc., pharmacological action is notable.Its chemical composition is more complicated, it has been determined that
Type of compounds mainly have：Alkaloids, polysaccharide, luxuriant and rich with fragrance class, bibenzyl parent nucleus class, wherein alkaloids, polysaccharide pharmacology are lived
Property is more notable.Polysaccharide constituents are that medicinal dendrobium plays the important substance basis that immunocompetence acts on, Dendrobium officinale polysaccharide energy
Peripheral leukocytes are significantly improved, promotes lymphocyte to produce migration inhibition factor, immunity of organisms can be effectively improved.2010
Version《Pharmacopeia》The quality standard of dendrobium candidum has been recorded first, and assay is carried out to polysaccharide and mannose.
As the health-care efficacy of dendrobium candidum by people is widely recognized and received, dendrobium officinale far can not expire
The demand of sufficient people.People have begun to use artificial cultivation dendrobium candidum, in the pseudo-wild cultivating method of dendrobium candidum, culture
Base is dendrobium candidum conditions on which persons or things depend for existence, different culture raw materials and with can be to dendrobium candidum growth, yield and activity
Composition etc. produces important influence.The culture medium of dendrobium candidum and existing pseudo-wild cultivating method in the prior art, are unfavorable for
The quality guarantee of the dendrobium candidum of output.Particularly it cannot be guaranteed that in dendrobium candidum polysaccharide content so that the iron finally given
Polyoses content is low in the skin stem of noble dendrobium.
The content of the invention
The purpose of the present invention, it is to provide a kind of pseudo-wild cultivating method of the high dendrobium candidum of polyoses content, present invention side
During method cultivation of dendrobium officinale, the dendrobium candidum quality better of final output, active constituent content height, the particularly content of polysaccharide
It is high.
What the present invention was realized in.
A kind of pseudo-wild cultivating method of the high dendrobium candidum of polyoses content, comprises the following steps：
（1）Once cultivate：Dendrobium candidum protocorm is taken, is cultivated in root media, is highly up to growing 1-2 piece true leaves
1.5-2.5cm dendrobium candidum seedling；
（2）Second incubation：Height is taken to be inoculated in seedling culture medium for 1.5-2.5cm, the dendrobium candidum seedling of the true leaf of piece containing 1-2
In, it is 20-25 DEG C in daytime temperature, under the conditions of 18-20 DEG C of night temperature, until growing into the seedling that 3-4cm is high, there is 3-5 bar adventitious roots；
（3）Tame and docile seedling：Seedling obtained by second incubation is positioned over culture 20-30 days in greenhouse, and then culture 7-9 days is continued in outdoor,
Obtain dendrobium candidum plant；
（4）Plantation：Select height above sea level 800-1600m, relative humidity 55%-70%, annual rainfall 1000-1500mm, winter temperature
More than 10 DEG C, 18-30 DEG C of season of growth temperature, 15-20 DEG C of rest period temperature, the stone of 10-15 DEG C of day and night temperature difference holding
Mountain and the excessive geomorphic province in mud mountain；In March~April, temperature reaches 20 DEG C~25 DEG C, and relative air humidity is 75%~
85%, dendrobium candidum plant is divided into one clump by 3 plants~5 plants, root is wrapped with moss, then puts stone recessed joint or stone into
In gap between block；
（5）Top dressing：In annual April~May, 5-10g fertilizer bags are placed in the root of each clump of dendrobium candidum；
（6）Watering：After cultivation in 5-7 days, 2-3 water is poured daily；
（7）The prevention and control of plant diseases, pest control：Monthly sprayed with the thiophanate methyl aqueous solution that concentration is 1-2 ‰ and carry out foliar control, insect prevention is adopted
Use fly net；
（8）Harvesting：It can be harvested after cultivation 1-2.
In the pseudo-wild cultivating method of the high dendrobium candidum of foregoing polyoses content, step（1）In, described culture of rootage
The main barrenwort 5-15 parts, aleppo avens 10-20 parts, honeysuckle 5-15 part, Polygonum multiflower knotweed 10-20 parts, white by calculating by weight of base
Splendid achnatherum 5-15 parts, Poria cocos 5-15 parts, peptone 1-10 parts, potato 1-10 parts, potato 1-5 parts and soya bean 5-15 parts are in the steps below
Prepared：
（1）Barrenwort, aleppo avens, honeysuckle, Polygonum multiflower knotweed, the bletilla striata and Poria cocos are taken, adds 16-18 times to measure after decocting boils 5-6h, filters, filter
Slag discards, and collects filtrate, obtains A product；
（2）Fetch earth beans and potato, add 5-6 times and measure water, break into slurry, obtain B product；
（3）Soya bean is taken, is ground into powder, obtains C product；
（4）A product, the mixing of B product, are heated to 50-60 DEG C, add C product and peptone, after stirring 5-10min, be cooled to 5-10 DEG C,
Produce.
In the pseudo-wild cultivating method of the high dendrobium candidum of foregoing polyoses content, described root media mainly by by
Barrenwort 8-12 parts of listed as parts by weight, aleppo avens 13-17 parts, honeysuckle 8-12 part, Polygonum multiflower knotweed 13-17 parts, bletilla striata 8-12 parts, Fu
Siberian cocklebur 8-12 parts, peptone 3-7 parts, potato 3-7 parts, potato 2-4 parts and soya bean 8-12 parts are prepared in the steps below：
（1）Barrenwort, aleppo avens, honeysuckle, Polygonum multiflower knotweed, the bletilla striata and Poria cocos are taken, adds 16-18 times to measure after decocting boils 5-6h, filters, filter
Slag discards, and collects filtrate, obtains A product；
（2）Fetch earth beans and potato, add 5-6 times and measure water, break into slurry, obtain B product；
（3）Soya bean is taken, is ground into powder, obtains C product；
（4）A product, the mixing of B product, are heated to 50-60 DEG C, add C product and peptone, after stirring 5-10min, be cooled to 5-10 DEG C,
Produce.
In the pseudo-wild cultivating method of the high dendrobium candidum of foregoing polyoses content, described root media mainly by by
15 parts of 10 parts of barrenwort, the aleppo avens of listed as parts by weight, 10 parts of honeysuckle, 15 parts of Polygonum multiflower knotweed, 10 parts of the bletilla striata, 10 parts of Poria cocos, albumen
10 parts of 5 parts of peptone, 5 parts of potato, 3 parts of potato and soya bean are prepared in the steps below：
（1）Barrenwort, aleppo avens, honeysuckle, Polygonum multiflower knotweed, the bletilla striata and Poria cocos are taken, after adding 17 times of amount decoctings to boil 5.5h, filtering, filter residue
Discard, collect filtrate, obtain A product；
（2）Fetch earth beans and potato, add 5.5 times of amount water, break into slurry, obtain B product；
（3）Soya bean is taken, is ground into powder, obtains C product；
（4）A product, the mixing of B product, are heated to 50-60 DEG C, add C product and peptone, after stirring 8min, be cooled to 5-10 DEG C, i.e.,
.
In the pseudo-wild cultivating method of the high dendrobium candidum of foregoing polyoses content, step（2）In, the seedling culture medium
Calculate by weight mainly by cooked maize mud 10-20 parts, Chinese yam mud 10-20 parts, taro paste 10-20 parts, agar 1-5 parts, dried orange peel
Powder 1-5 parts and kudzu-vine root powder 1-5 parts mix, and produce.
In the pseudo-wild cultivating method of the high dendrobium candidum of foregoing polyoses content, the seedling culture medium is by weight
Calculate and mainly mixed by 3 parts of 15 parts of cooked maize mud, 15 parts of Chinese yam mud, 15 parts of taro paste, 3 parts of agar, 3 parts of tangerine peel powder and kudzu-vine root powder,
Produce.
In the pseudo-wild cultivating method of the high dendrobium candidum of foregoing polyoses content, step（5）In, described fertilizer bag is pressed
Listed as parts by weight, mainly by 40-50% mud 50-60 parts, mashed potatoes 5-15 parts, peptone 0.1-0.5 parts, agar 0.1-0.5
Part, vitamin B1 0.1-0.5 parts, malt 0.1-0.5 parts and azedarach powder 1-10 parts mix, and produce.
In the pseudo-wild cultivating method of the high dendrobium candidum of foregoing polyoses content, described fertilizer calculates by weight,
Mainly by 40-50% 0.3 part of 0.3 part of 0.3 part of 10 parts of 55 parts of mud, mashed potatoes, peptone, agar, vitamin B1, malt
0.3 part and the 5 parts of mixings of azedarach powder, are produced.
Applicant has carried out substantial amounts of experimental study to the present invention, and part Experiment is as follows：
The polyoses content of experimental example 1 investigates
1 materials and methods
1.1 material
1.1.1 dendrobium candidum 1：After being cultivated by embodiment 1, harvesting obtains dendrobium candidum 1.
1.1.2 dendrobium candidum 2：During cultivation, without barrenwort, aleppo avens, honeysuckle, Polygonum multiflower knotweed, the bletilla striata in root media
And Poria cocos；After remaining method is cultivated with embodiment 1, harvesting obtains dendrobium candidum 2.
1.2 instruments and reagent
Dextrose standard sample [glucose of D mono- (+) one]：KAYON companies；Methanol (chromatographically pure)：Close europeanized learn of Tianjin section is tried
Agent Co., Ltd；Remaining reagent is that analysis is pure；Phenol：Shanghai Ling Feng chemical reagent Co., Ltd；Sulfuric acid：Shanghai Ling Feng chemistry
Reagent Co., Ltd.
2 methods
The preparation of 2.1 need testing solutions
2.1.1 the preparation of need testing solution 1：
Take dendrobium candidum of the present invention（Dendrobium candidum 1）Sample, 50 DEG C are put in baking oven, dry, pulverize, No. three sieves are crossed, after taking filtering
Medicinal powder, solvent is volatilized, add the mL of water 2000, be heated to reflux 2 hours, let cool, be transferred in 250 mL measuring bottles, with a small amount of
Moisture time washing container, washing lotion are incorporated in same measuring bottle, add water to scale, shake up, and filter, and precision measures the mL of subsequent filtrate 2, put
In 15 mL centrifuge tubes, precision adds 95% ethanol, shakes up, and refrigerates 1 hour, takes out, and centrifuges 20 minutes, abandoning supernatant, sinks
Form sediment and add hot water dissolving, be transferred in 25 mL measuring bottles, let cool, add water to scale, shake up, produce need testing solution 1.
2.1.2 the preparation of need testing solution 2
The sample of dendrobium candidum 2 is taken, 50 DEG C is put in baking oven, dry, pulverize, No. three sieves is crossed, takes medicinal powder after filtering, volatilize molten
Agent, add the mL of water 2000, be heated to reflux 2 hours, let cool, be transferred in 250 mL measuring bottles, with a small amount of moisture time washing container,
Washing lotion is incorporated in same measuring bottle, adds water to scale, is shaken up, and filtration, precision measures the mL of subsequent filtrate 2, put in 15 mL centrifuge tubes, essence
95% ethanol of close addition, shakes up, and refrigerates 1 hour, takes out, and centrifuges 20 minutes, abandoning supernatant, precipitation plus hot water dissolving, turns
Move in 25 mL measuring bottles, let cool, add water to scale, shake up, produce need testing solution 2.
The preparation of 2.2 reference substance solutions
It is appropriate to the DEXTROSE ANHYDROUS of constant weight that precision weighs 105 DEG C of dryings, adds water that 99 mg/L solution are made, standby.
It is prepared by 2.3 standard curves
Precision measures the m L of reference substance solution 0.2,0.4,0.6,0.8,1.0 and put in 10m L tool plug test tubes, adds water to
1.0mL, precision add 5% phenol solution 1mL (I is now prepared before use), shaken up, then the accurate addition m L of sulfuric acid 5, shake up, put
Heated 20 minutes in boiling water bath, take out, be put into ice-water bath and cool down 5 minutes, using corresponding reagent as blank, in 488 nm wavelength
Place's measure absorbance, using absorbency Y as ordinate, concentration X (g/L) is abscissa, draws standard curve, and regression equation is：Y
=11.772X -0.1492, r=0.9995.As a result show that polysaccharide linear relationship in the range of 0.02-0.10 g/L is good
It is good.
2.4 determination method
Precision measures need testing solution 1 and each 0.5 mL of need testing solution 2 respectively, puts in 10 mL tool plug test tubes, adds water to 1mL
, by the method under the preparation of " 2.3 " standard curve, from " precision adds 5% phenol solution 1m L ", determining extinction in accordance with the law
Degree, the content of DEXTROSE ANHYDROUS in need testing solution is read from standard curve, calculate, produce.
2.5 precision test
Precision draws 6 parts, every part of 0.6 m L of glucose control product solution, and absorbance is determined according to the method under " 2.4 " item.Survey
Determine result, RSD is respectively 1.60% and 1.62%, and precision is good.
2.6 replica test
6 parts of need testing solutions 1 and need testing solution 2 are prepared as stated above, according to determining absorbance as stated above.Measure knot
Fruit, RSD are respectively 2.70% and 2.71%, and repeatability is good.
2.7 stability test
Same need testing solution 1 and need testing solution 2 are taken respectively, is placed at room temperature, respectively at 10min, 30min, 1 h, 2
H, absorbance is determined as stated above after 4 h, 6 h.Measurement result, RSD are respectively 2.70% and 2.73%, show that test sample is molten
Liquid 1 and need testing solution 2 are stable in 6 hours.
2.8 average recoveries are tested
6 parts, every part of about 1.65g of 1 powder of dendrobium candidum of known content (12.5%) is weighed, it is accurately weighed.It is accurate to add grape
2 mL of sugared reference substance solution (concentration is 0.099 g/L), prepare need testing solution 1, determine as stated above as stated above,
Polyoses content is calculated, as a result average recovery rate is 94.9%, RSD 2.7%.
6 parts, every part of about 1.65g of 2 powder of dendrobium candidum of known content (12.5%) is weighed, it is accurately weighed.It is accurate to add
Glucose control product solution (concentration is 0.099 g/L) 2 mL, prepare need testing solution 2, survey as stated above as stated above
It is fixed, polyoses content is calculated, as a result average recovery rate is 93.9%, RSD 2.6%.
2.9 samples determine
By the preparation method and condition determination of above-mentioned need testing solution 1 and need testing solution 2, determine 6 parts of samples of dendrobium candidum 1 and
The content of polysaccharide in 6 parts of samples of dendrobium candidum 2, result is recorded, results averaged, the results are shown in Table 1.
As seen from table, the inventive method cultivates obtained dendrobium candidum, the content of polysaccharide in the dendrobium candidum finally harvested
It is higher.The quality better of dendrobium candidum.
Compared with prior art, during the inventive method cultivation of dendrobium officinale, the dendrobium candidum quality better of final output, effectively
Component content is high, particularly the height of the content of polysaccharide.
Embodiment
Embodiment 1.
A kind of pseudo-wild cultivating method of the high dendrobium candidum of polyoses content, comprises the following steps：
（1）Once cultivate：Dendrobium candidum protocorm is taken, is cultivated in root media, is highly up to growing 1-2 piece true leaves
1.5-2.5cm dendrobium candidum seedling；
（2）Second incubation：Height is taken to be inoculated in seedling culture medium for 1.5-2.5cm, the dendrobium candidum seedling of the true leaf of piece containing 1-2
In, it is 20-25 DEG C in daytime temperature, under the conditions of 18-20 DEG C of night temperature, until growing into the seedling that 3-4cm is high, there is 3-5 bar adventitious roots；
（3）Tame and docile seedling：Seedling obtained by second incubation is positioned over culture 25 days in greenhouse, and then culture 8 days is continued in outdoor, obtains iron sheet
Dendrobium plant；
（4）Plantation：Select height above sea level 800-1600m, relative humidity 55%-70%, annual rainfall 1000-1500mm, winter temperature
More than 10 DEG C, 18-30 DEG C of season of growth temperature, 15-20 DEG C of rest period temperature, the stone of 10-15 DEG C of day and night temperature difference holding
Mountain and the excessive geomorphic province in mud mountain；In March~April, temperature reaches 20 DEG C~25 DEG C, and relative air humidity is 75%~
85%, dendrobium candidum plant is divided into one clump by 3 plants~5 plants, root is wrapped with moss, then puts stone recessed joint or stone into
In gap between block；
（5）Top dressing：In annual April~May, 5-10g fertilizer bags are placed in the root of each clump of dendrobium candidum；
（6）Watering：After cultivation in 6 days, 3 water are poured daily；
（7）The prevention and control of plant diseases, pest control：Monthly sprayed with the thiophanate methyl aqueous solution that concentration is 1.5 ‰ and carry out foliar control, insect prevention is adopted
Use fly net；
（8）Harvesting：It can be harvested after cultivation 1-2.
Described root media by barrenwort 10kg, aleppo avens 15kg, honeysuckle 10kg, Polygonum multiflower knotweed 15kg, bletilla striata 10kg,
Poria cocos 10kg, peptone 5kg, potato 5kg, potato 3kg and soya bean 10kg are prepared in the steps below：
（1）Barrenwort, aleppo avens, honeysuckle, Polygonum multiflower knotweed, the bletilla striata and Poria cocos are taken, after adding 17 times of amount decoctings to boil 5.5h, filtering, filter residue
Discard, collect filtrate, obtain A product；
（2）Fetch earth beans and potato, add 5.5 times of amount water, break into slurry, obtain B product；
（3）Soya bean is taken, is ground into powder, obtains C product；
（4）A product, the mixing of B product, are heated to 50-60 DEG C, add C product and peptone, after stirring 8min, be cooled to 5-10 DEG C, i.e.,
.
The seedling culture medium is by cooked maize mud 15kg, Chinese yam mud 15kg, taro paste 15kg, agar 3kg, tangerine peel powder 3kg
Mix, produce with kudzu-vine root powder 3kg.
Described fertilizer bag is by 45% mud 55kg, mashed potatoes 10kg, peptone 0.3kg, agar 0.3kg, vitamin B1
0.3kg, malt 0.3kg and azedarach powder 5kg are mixed, and are produced.
Embodiment 2.
A kind of pseudo-wild cultivating method of the high dendrobium candidum of polyoses content, comprises the following steps：
（1）Once cultivate：Dendrobium candidum protocorm is taken, is cultivated in root media, is highly up to growing 1-2 piece true leaves
1.5-2.5cm dendrobium candidum seedling；
（2）Second incubation：Height is taken to be inoculated in seedling culture medium for 1.5-2.5cm, the dendrobium candidum seedling of the true leaf of piece containing 1-2
In, it is 20-25 DEG C in daytime temperature, under the conditions of 18-20 DEG C of night temperature, until growing into the seedling that 3-4cm is high, there is 3-5 bar adventitious roots；
（3）Tame and docile seedling：Seedling obtained by second incubation is positioned over culture 30 days in greenhouse, and then culture 9 days is continued in outdoor, obtains iron sheet
Dendrobium plant；
（4）Plantation：Select height above sea level 800-1600m, relative humidity 55%-70%, annual rainfall 1000-1500mm, winter temperature
More than 10 DEG C, 18-30 DEG C of season of growth temperature, 15-20 DEG C of rest period temperature, the stone of 10-15 DEG C of day and night temperature difference holding
Mountain and the excessive geomorphic province in mud mountain；In March~April, temperature reaches 20 DEG C~25 DEG C, and relative air humidity is 75%~
85%, dendrobium candidum plant is divided into one clump by 3 plants~5 plants, root is wrapped with moss, then puts stone recessed joint or stone into
In gap between block；
（5）Top dressing：In annual April~May, 5-10g fertilizer bags are placed in the root of each clump of dendrobium candidum；
（6）Watering：After cultivation in 7 days, 2 water are poured daily；
（7）The prevention and control of plant diseases, pest control：Monthly sprayed with the thiophanate methyl aqueous solution that concentration is 1-2 ‰ and carry out foliar control, insect prevention is adopted
Use fly net；
（8）Harvesting：Cultivation can harvest after 2 years.
Described root media by barrenwort 12kg, aleppo avens 17kg, honeysuckle 12kg, Polygonum multiflower knotweed 17kg, bletilla striata 12kg,
Poria cocos 12kg, peptone 7kg, potato 7kg, potato 4kg and soya bean 12kg are prepared in the steps below：
（1）Barrenwort, aleppo avens, honeysuckle, Polygonum multiflower knotweed, the bletilla striata and Poria cocos are taken, after adding 18 times of amount decoctings to boil 6h, filtering, filter residue is abandoned
Go, collect filtrate, obtain A product；
（2）Fetch earth beans and potato, add 6 times of amount water, break into slurry, obtain B product；
（3）Soya bean is taken, is ground into powder, obtains C product；
（4）A product, the mixing of B product, are heated to 50-60 DEG C, add C product and peptone, after stirring 10min, be cooled to 5-10 DEG C, i.e.,
.
The seedling culture medium is by cooked maize mud 20kg, Chinese yam mud 20kg, taro paste 20kg, agar 5kg, tangerine peel powder 5kg
Mix, produce with kudzu-vine root powder 5kg.
Described fertilizer bag is by 50% mud 60kg, mashed potatoes 15kg, peptone 0.5kg, agar 0.5kg, vitamin B1
0.5kg, malt 0.5kg and azedarach powder 10kg are mixed, and are produced.
Embodiment 3.
A kind of pseudo-wild cultivating method of the high dendrobium candidum of polyoses content, comprises the following steps：
（1）Once cultivate：Dendrobium candidum protocorm is taken, is cultivated in root media, is highly up to growing 1-2 piece true leaves
1.5-2.5cm dendrobium candidum seedling；
（2）Second incubation：Height is taken to be inoculated in seedling culture medium for 1.5-2.5cm, the dendrobium candidum seedling of the true leaf of piece containing 1-2
In, it is 20-25 DEG C in daytime temperature, under the conditions of 18-20 DEG C of night temperature, until growing into the seedling that 3-4cm is high, there is 3-5 bar adventitious roots；
（3）Tame and docile seedling：Seedling obtained by second incubation is positioned over culture 20 days in greenhouse, and then culture 7 days is continued in outdoor, obtains iron sheet
Dendrobium plant；
（4）Plantation：Select height above sea level 800-1600m, relative humidity 55%-70%, annual rainfall 1000-1500mm, winter temperature
More than 10 DEG C, 18-30 DEG C of season of growth temperature, 15-20 DEG C of rest period temperature, the stone of 10-15 DEG C of day and night temperature difference holding
Mountain and the excessive geomorphic province in mud mountain；In March~April, temperature reaches 20 DEG C~25 DEG C, and relative air humidity is 75%~
85%, dendrobium candidum plant is divided into one clump by 3 plants~5 plants, root is wrapped with moss, then puts stone recessed joint or stone into
In gap between block；
（5）Top dressing：In annual April~May, 5-10g fertilizer bags are placed in the root of each clump of dendrobium candidum；
（6）Watering：After cultivation in 7 days, 2 water are poured daily；
（7）The prevention and control of plant diseases, pest control：Monthly sprayed with the thiophanate methyl aqueous solution that concentration is 1 ‰ and carry out foliar control, insect prevention uses
Fly net；
（8）Harvesting：Cultivation can harvest after 1 year.
Described root media is by barrenwort 8kg, aleppo avens 13kg, honeysuckle 8kg, Polygonum multiflower knotweed 13kg, the bletilla striata 8kg, Fu
Siberian cocklebur 8kg, peptone 3kg, potato 3kg, potato 2kg and soya bean 8kg are prepared in the steps below：
（1）Barrenwort, aleppo avens, honeysuckle, Polygonum multiflower knotweed, the bletilla striata and Poria cocos are taken, after adding 16 times of amount decoctings to boil 5h, filtering, filter residue is abandoned
Go, collect filtrate, obtain A product；
（2）Fetch earth beans and potato, add 5 times of amount water, break into slurry, obtain B product；
（3）Soya bean is taken, is ground into powder, obtains C product；
（4）A product, the mixing of B product, are heated to 50-60 DEG C, add C product and peptone, after stirring 5min, be cooled to 5-10 DEG C, i.e.,
.
The seedling culture medium is by cooked maize mud 10kg, Chinese yam mud 10kg, taro paste 10kg, agar 1kg, tangerine peel powder 1kg
Mix, produce with kudzu-vine root powder 1kg.
Described fertilizer bag is by 40% mud 50kg, mashed potatoes 5kg, peptone 0.1kg, agar 0.1kg, vitamin B1
0.1kg, malt 0.1kg and azedarach powder 1kg are mixed, and are produced.
Claims (8)
- A kind of 1. pseudo-wild cultivating method of the high dendrobium candidum of polyoses content, it is characterised in that：Comprise the following steps：Once cultivate：Dendrobium candidum protocorm is taken, is cultivated in root media, until it is highly 1.5- to grow 1-2 pieces true leaf 2.5cm dendrobium candidum seedling；（2）Second incubation：Height is taken to be inoculated in seedling culture medium for 1.5-2.5cm, the dendrobium candidum seedling of the true leaf of piece containing 1-2 In, it is 20-25 DEG C in daytime temperature, under the conditions of 18-20 DEG C of night temperature, until growing into the seedling that 3-4cm is high, there is 3-5 bar adventitious roots；（3）Tame and docile seedling：Seedling obtained by second incubation is positioned over culture 20-30 days in greenhouse, and then culture 7-9 days is continued in outdoor, Obtain dendrobium candidum plant；（4）Plantation：Select height above sea level 800-1600m, relative humidity 55%-70%, annual rainfall 1000-1500mm, winter temperature More than 10 DEG C, 18-30 DEG C of season of growth temperature, 15-20 DEG C of rest period temperature, the stone of 10-15 DEG C of day and night temperature difference holding Mountain and the excessive geomorphic province in mud mountain；In March~April, temperature reaches 20 DEG C~25 DEG C, and relative air humidity is 75%~ 85%, dendrobium candidum plant is divided into one clump by 3 plants~5 plants, root is wrapped with moss, then puts stone recessed joint or stone into In gap between block；（5）Top dressing：In annual April~May, 5-10g fertilizer bags are placed in the root of each clump of dendrobium candidum；（6）Watering：After cultivation in 5-7 days, 2-3 water is poured daily；（7）The prevention and control of plant diseases, pest control：Monthly sprayed with the thiophanate methyl aqueous solution that concentration is 1-2 ‰ and carry out foliar control, insect prevention is adopted Use fly net；（8）Harvesting：It can be harvested after cultivation 1-2.
- 2. the pseudo-wild cultivating method of the high dendrobium candidum of polyoses content as claimed in claim 1, it is characterised in that：Step （1）In, the main barrenwort 5-15 part, aleppo avens 10-20 parts, honeysuckle 5-15 by calculating by weight of described root media Part, Polygonum multiflower knotweed 10-20 parts, bletilla striata 5-15 parts, Poria cocos 5-15 parts, peptone 1-10 parts, potato 1-10 parts, potato 1-5 parts and Soya bean 5-15 parts are prepared in the steps below：（1）Barrenwort, aleppo avens, honeysuckle, Polygonum multiflower knotweed, the bletilla striata and Poria cocos are taken, adds 16-18 times to measure after decocting boils 5-6h, filters, filter Slag discards, and collects filtrate, obtains A product；（2）Fetch earth beans and potato, add 5-6 times and measure water, break into slurry, obtain B product；（3）Soya bean is taken, is ground into powder, obtains C product；（4）A product, the mixing of B product, are heated to 50-60 DEG C, add C product and peptone, after stirring 5-10min, be cooled to 5-10 DEG C, Produce.
- 3. the pseudo-wild cultivating method of the high dendrobium candidum of polyoses content as claimed in claim 2, it is characterised in that：Described Root media mainly by calculate by weight barrenwort 8-12 parts, aleppo avens 13-17 parts, honeysuckle 8-12 part, Polygonum multiflower knotweed 13- 17 parts, bletilla striata 8-12 parts, Poria cocos 8-12 parts, peptone 3-7 parts, potato 3-7 parts, potato 2-4 parts and soya bean 8-12 parts are pressed Step is stated to be prepared：（1）Barrenwort, aleppo avens, honeysuckle, Polygonum multiflower knotweed, the bletilla striata and Poria cocos are taken, adds 16-18 times to measure after decocting boils 5-6h, filters, filter Slag discards, and collects filtrate, obtains A product；（2）Fetch earth beans and potato, add 5-6 times and measure water, break into slurry, obtain B product；（3）Soya bean is taken, is ground into powder, obtains C product；（4）A product, the mixing of B product, are heated to 50-60 DEG C, add C product and peptone, after stirring 5-10min, be cooled to 5-10 DEG C, Produce.
- 4. the pseudo-wild cultivating method of the high dendrobium candidum of polyoses content as claimed in claim 3, it is characterised in that：Described Root media mainly by calculate by weight 15 parts of 10 parts of barrenwort, aleppo avens, 10 parts of honeysuckle, 15 parts of Polygonum multiflower knotweed, the bletilla striata 10 parts of 10 parts, 10 parts of Poria cocos, 5 parts of peptone, 5 parts of potato, 3 parts of potato and soya bean are prepared in the steps below：（1）Barrenwort, aleppo avens, honeysuckle, Polygonum multiflower knotweed, the bletilla striata and Poria cocos are taken, after adding 17 times of amount decoctings to boil 5.5h, filtering, filter residue Discard, collect filtrate, obtain A product；（2）Fetch earth beans and potato, add 5.5 times of amount water, break into slurry, obtain B product；（3）Soya bean is taken, is ground into powder, obtains C product；（4）A product, the mixing of B product, are heated to 50-60 DEG C, add C product and peptone, after stirring 8min, be cooled to 5-10 DEG C, i.e., .
- 5. the pseudo-wild cultivating method of the high dendrobium candidum of polyoses content as claimed in claim 1, it is characterised in that：Step （2）In, the seedling culture medium calculates mainly by cooked maize mud 10-20 parts, Chinese yam mud 10-20 parts, taro paste 10- by weight 20 parts, agar 1-5 parts, tangerine peel powder 1-5 parts and kudzu-vine root powder 1-5 parts mix, and produce.
- 6. the pseudo-wild cultivating method of the high dendrobium candidum of polyoses content as claimed in claim 5, it is characterised in that：The children Seedling culture medium calculates mainly by 15 parts of cooked maize mud, 15 parts of Chinese yam mud, 15 parts of taro paste, 3 parts of agar, tangerine peel powder 3 by weight The 3 parts of mixings of part and kudzu-vine root powder, are produced.
- 7. the pseudo-wild cultivating method of the high dendrobium candidum of polyoses content as claimed in claim 1, it is characterised in that：Step （5）In, described fertilizer bag calculates by weight, mainly by 40-50% mud 50-60 parts, mashed potatoes 5-15 parts, peptone 0.1-0.5 parts, agar 0.1-0.5 parts, vitamin B1 0.1-0.5 parts, malt 0.1-0.5 parts and azedarach powder 1-10 parts mix, Produce.
- 8. the pseudo-wild cultivating method of the high dendrobium candidum of polyoses content as claimed in claim 7, it is characterised in that：Described Fertilizer bag calculates by weight, mainly by 40-50% 0.3 part of 0.3 part of 10 parts of 55 parts of mud, mashed potatoes, peptone, agar, dimension 5 parts of raw plain 0.3 part of B1,0.3 part of malt and azedarach powder mixings, are produced.
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