CN107616084A - A kind of pseudo-wild cultivating method of the high dendrobium candidum of stem of noble dendrobium alkali content - Google Patents
A kind of pseudo-wild cultivating method of the high dendrobium candidum of stem of noble dendrobium alkali content Download PDFInfo
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Abstract
The invention discloses a kind of pseudo-wild cultivating method of the high dendrobium candidum of stem of noble dendrobium alkali content, including seed to sprout；Seedling culture；Paste tree planting：Plantation selection strikes off the deep barks of 3 5cm in the tree on spring, fir, by 30 40cm, and the root and stem of certain plants applies slurry mixture, then fill at broken skin or set at lobe ditch for 1 clump by 5 10 plants and be adjacent to bark, then carry out one layer of straw, bundled with thin bamboo strips used for weaving, during binding, root system is only tied up, exposes stem foot；Weeding：Weeding every year 23 times, carried out in the annual Spring Equinox to Clear and Bright, remove weeds and dry branches and fallen leaves；Top dressing：First time top dressing sprays Liquid Fertilizer 57 days before Clear and Bright, is pasted with fertilizer in root within second 57 days after the Beginning of Winter；Watering：After cultivation in 57 days, 23 water are poured daily, bark humid control is 75 85%；Harvesting：It can be harvested after cultivating 12 years.During the inventive method method cultivation of dendrobium officinale, the dendrobium candidum quality better of final output, active constituent content is high, particularly the height of the content of dendrobine.
Description
Technical field
The present invention relates to a kind of pseudo-wild cultivating method of dendrobium candidum, the high iron sheet stone of particularly a kind of stem of noble dendrobium alkali content
The pseudo-wild cultivating method of dry measure used in former times.
Background technology
Dendrobium candidum nickname iron sheet orchid, bracketplant, Yunnan iron sheet, iron sheet bracketplant, it is orchid family Dendrobium herbaceos perennial,
Dendrobium candidum is the top grade of Shihu " medicinal materials, is known as the laudatory title of " gold in Chinese celestial grass-medicine ", is used as medicine with stem, contains polysaccharide, stone
The active ingredients such as dry measure used in former times alkali, alkaloid, with nourishing Yin and clearing heat, the beneficial stomach that promotes the production of body fluid, wet one's whistle improving eyesight and other effects, in antitumor, raising human body
Immunity, treatment enterogastric diseases, anti-aging, anti-oxidant, platelet aggregation-against, reduction blood glucose, treatment cataract etc. are
There is good efficacy.
Modern pharmacology research shows that dendrobium candidum has humidification to non-specific and specific immunity, anti-swollen
Knurl, treatment that is anti-oxidant, reducing blood glucose and cataract etc., pharmacological action is notable.Its chemical composition is more complicated, it has been determined that
Type of compounds mainly have：Alkaloids, polysaccharide, luxuriant and rich with fragrance class, bibenzyl parent nucleus class, wherein alkaloids, polysaccharide pharmacology are lived
Property is more notable.Stem of noble dendrobium alkaline sesquiterpenoids alkaloid is Dendrobium
Plant is peculiar, and wherein dendrobine is to find earliest, the alkaloid of most study；HERBA DENDROBII alkali and non-steroid anti-inflammatory drug
Pharmacological action is similar, has the effects such as immunological regulation, anti-inflammatory, expansion blood vessel.
As the health-care efficacy of dendrobium candidum by people is widely recognized and received, dendrobium officinale far can not expire
The demand of sufficient people.People have begun to use artificial cultivation dendrobium candidum, and in the cultural method of dendrobium candidum, culture medium is iron
Skin stem of noble dendrobium conditions on which persons or things depend for existence, different culture raw materials and with can be to dendrobium candidum growth, yield and active component etc.
Produce important influence.The culture medium of dendrobium candidum and existing cultural method in the prior art, it is unfavorable for the iron sheet stone of output
The quality guarantee of dry measure used in former times.Particularly it cannot be guaranteed that stem of noble dendrobium alkali content in dendrobium candidum so that the stem of noble dendrobium in the dendrobium candidum finally given
Alkali content is low.
The content of the invention
The purpose of the present invention, it is to provide a kind of pseudo-wild cultivating method of the high dendrobium candidum of stem of noble dendrobium alkali content, the inventive method
During method cultivation of dendrobium officinale, the dendrobium candidum quality better of final output, active constituent content is high, particularly the content of dendrobine
Height.
What the present invention was realized in.A kind of pseudo-wild cultivating method of the high dendrobium candidum of stem of noble dendrobium alkali content, including with
Lower step：
（1）Seed is sprouted：Seeds of Dendrobium Candidum is taken, after cleaning, 70-80% ethanol disinfection 5- is used on superclean bench
After 15min, then with aseptic water washing 3-4 times, it is placed on the filter paper of sterilizing after blotting, is inoculated on germination medium, in daytime temperature
For 20-25 DEG C, under the conditions of 18-20 DEG C of night temperature, light culture, until seed is sprouted；
（2）Seedling culture：Treat seed sprout, grow into 3-4cm it is high when, when having 3-5 bar adventitious roots, remove, be positioned in greenhouse
Culture 10-15 days, then outdoor continue culture 7-9 days, obtain dendrobium candidum seedling；
（3）Paste tree planting：Plantation selection is struck off the deep barks of 3-5cm by 30-40cm in the tree on spring, fir, and the root and stem of certain plants applies
Slurry mixture, then by 5-10 strains be 1 clump fill at broken skin or tree lobe ditch at be adjacent to bark, then carry out one layer of straw, use bamboo
The thin bamboo strip bundlees, and during binding, only ties up root system, exposes stem foot；
（4）Weeding：Weeding every year 2-3 times, carried out in the annual Spring Equinox to Clear and Bright, remove weeds and dry branches and fallen leaves；
（5）Top dressing：First time top dressing sprays Liquid Fertilizer 5-7 days before Clear and Bright, is pasted with fertilizer within second 5-7 days after the Beginning of Winter
Root；
（6）Watering：After cultivation in 5-7 days, 2-3 water is poured daily, bark humid control is in 75-85%；
（7）Harvesting：It can be harvested after cultivation 1-2.
In the pseudo-wild cultivating method of the high dendrobium candidum of foregoing stem of noble dendrobium alkali content, the step（1）In, the sprouting
Culture medium mainly by calculate by weight sucrose 3-6 parts, sweet potato mash 10-20 parts, soybean residue 5-15 parts, Polygonum multiflower knotweed 5-15 parts,
Radix bupleuri 10-20 parts, coptis 5-15 parts, subprostrate sophora 5-15 parts, bighead atractylodes rhizome 10-20 parts and Chinese ephedra 10-20 parts are made as follows：Night
After handing over rattan, radix bupleuri, the coptis, subprostrate sophora, the bighead atractylodes rhizome and Chinese ephedra to add the 10-12 times of decocting measured to boil 2-3h, filtering, filter residue is taken, dried, powder
Coarse granule is broken into, coarse granule is mixed with sucrose, sweet potato mash, soybean residue, produced.
In the pseudo-wild cultivating method of the high dendrobium candidum of foregoing stem of noble dendrobium alkali content, the germination medium mainly by by
5 parts of the sucrose of listed as parts by weight, 15 parts of sweet potato mash, 10 parts of soybean residue, 10 parts of Polygonum multiflower knotweed, 15 parts of radix bupleuri, 10 parts of the coptis, subprostrate sophora
15 parts of 10 parts, 15 parts of the bighead atractylodes rhizome and Chinese ephedra are made as follows：Polygonum multiflower knotweed, radix bupleuri, the coptis, subprostrate sophora, the bighead atractylodes rhizome and Chinese ephedra add 11
After 2.5h is boiled in the decocting of amount again, filtering, filter residue is taken, dry, pulverize into coarse granule, coarse granule mixes with sucrose, sweet potato mash, soybean residue
It is even, produce.
In the pseudo-wild cultivating method of the high dendrobium candidum of foregoing stem of noble dendrobium alkali content, step（3）In, the mud mixing
Thing calculates by weight, mainly by wall fern 20-30 parts, 60-70% mud 40-50 parts, chicken manure 20-30 parts, lotus leaf slag 5-15
Part, mulberry leaf slag 5-15 parts, red sage root slag 5-15 parts and motherwort slag 5-15 parts mix, and produce.
In the pseudo-wild cultivating method of the high dendrobium candidum of foregoing stem of noble dendrobium alkali content, the slurry mixture is by weight
Calculate, mainly by 25 parts of the wall fern, 60-70% 10 parts of 10 parts of 25 parts of 45 parts of mud, chicken manure, lotus leaf slag, mulberry leaf slag, red sage root slag
10 parts and the 10 parts of mixings of motherwort slag, are produced.
In the pseudo-wild cultivating method of the high dendrobium candidum of foregoing stem of noble dendrobium alkali content, described lotus leaf slag；It is in lotus leaf
Add 5-6 times to measure decocting and boil 40-50min, decoction liquor filtering, take filter residue, produce；Described mulberry leaf slag；It is to add 5-6 times in mulberry leaf to measure
40-50min is boiled in decocting, decoction liquor filtering, is taken filter residue, is produced；Described red sage root slag；It is to add 5-6 times to measure decocting in the red sage root to boil 40-
50min, decoction liquor filtering, takes filter residue, produces；Described motherwort slag；It is to add 5-6 times to measure decocting in motherwort to boil 40-
50min, decoction liquor filtering, takes filter residue, produces.
In the pseudo-wild cultivating method of the high dendrobium candidum of foregoing stem of noble dendrobium alkali content, step（5）In, described Liquid Fertilizer
Calculate by weight, mainly mixed, produced by cow dung 10-20 parts, urea 0.1-0.5 parts and water 90-100 parts.
In the pseudo-wild cultivating method of the high dendrobium candidum of foregoing stem of noble dendrobium alkali content, described Liquid Fertilizer is by weight
Calculate, mainly by 95 parts of 15 parts of cow dung, 0.3 part of urea and water mixings, produce.
In the pseudo-wild cultivating method of the high dendrobium candidum of foregoing stem of noble dendrobium alkali content, step（5）In, described fertilizer is pressed
Listed as parts by weight, mainly by 40-50% mud 50-60 parts, mashed potatoes 5-15 parts, peptone 0.1-0.5 parts, agar 0.1-0.5
Part, vitamin B1 0.1-0.5 parts, malt 0.1-0.5 parts and tangerine peel powder 1-10 parts mix, and produce.
In the pseudo-wild cultivating method of the high dendrobium candidum of foregoing stem of noble dendrobium alkali content, described fertilizer is by weight
Calculate, mainly by 40-50% 0.3 part of 0.3 part of 0.3 part of 10 parts of 55 parts of mud, mashed potatoes, peptone, agar, vitamin B1, wheat
The 5 parts of mixings of 0.3 part of bud and tangerine peel powder, are produced.
Applicant has carried out substantial amounts of experimental study to the present invention, and part Experiment is as follows：
The stem of noble dendrobium alkali content of experimental example 1 investigates
1 materials and methods
1.1 material
1.1.1 dendrobium candidum 1：Cultivated by embodiment 1, harvesting obtains dendrobium candidum 1.
1.1.2 dendrobium candidum 2：During cultivation, in germination medium without Polygonum multiflower knotweed, radix bupleuri, the coptis, subprostrate sophora, the bighead atractylodes rhizome and
Chinese ephedra, after remaining method is cultivated with embodiment 1, harvesting obtains dendrobium candidum 2.
1.2 instruments and reagent
Dendrobine standard items：Chengdu Man Site Bioisystech Co., Ltd, MUST-12080608.A E240 electronic balances：It is auspicious
Scholar MEITLER；HH-s digital control constant temperature water-baths：Jinhua Wenhua science and education laboratory apparatus factory；The Rotary Evaporators of BM 510：Japan
YAM ATO companies；6890N gas chromatographs：Pacify in the U.S.
Jie Lun companies；UV-2450 ultraviolet specrophotometers：Japanese Shimadzu Corporation；Methanol (chromatographically pure)：Tianjin section is close europeanized
Learn reagent Co., Ltd；Remaining reagent is that analysis is pure；Phenol：Shanghai Ling Feng chemical reagent Co., Ltd；Sulfuric acid：Shanghai Ling Feng
Chemical reagent Co., Ltd.
2 methods
The preparation of 2.1 need testing solutions
2.1.1 the preparation of need testing solution 1：
Take dendrobium candidum of the present invention（Dendrobium candidum 1）Sample, 50 DEG C are put in baking oven, dry, pulverize, No. three sieves are crossed, after taking filtering
Medicinal powder 3.34g, add the ethanol of 200mL 90%, flow back 1 hour, filtering, pour out supernatant, then add 100mL90% ethanol, return
Stream 1 hour, supernatant is poured out, merge above-mentioned 2 supernatants, put Rotary Evaporators concentration, 50 DEG C of water-bath is evaporated, and dry product adds
Methanol dissolves, and is settled to 25m L, produces need testing solution 1.
2.1.2 the preparation of need testing solution 2
The sample of dendrobium candidum 2 is taken, 50 DEG C is put in baking oven, dry, pulverize, No. three sieves is crossed, takes medicinal powder 3.34g after filtering, add
The ethanol of 200mL 90%, flow back 1 hour, filtering, pour out supernatant, then add 100mL90% ethanol, flow back 1 hour, pour out supernatant
Liquid, merge above-mentioned 2 supernatants, put Rotary Evaporators concentration, 50 DEG C of water-bath is evaporated, and dry product adds methanol to dissolve, and is settled to
25m L, produce need testing solution 2.
The preparation of 2.2 reference substance solutions
Precision weighs dendrobine reference substance 50.01lmg into 100mL measuring bottles, adds methanol constant volume to shake up to scale, obtain 500.1m
G/L dendrobine reference substance mother liquors.50.01m g/L solution, it is standby as reference substance solution.
2.3 chromatographic condition
Chromatographic column：D B-1 capillary columns (30mm × 0.25mm, 0.25um)；Temperature programming：Initial temperature is 80 DEG C, with
10 DEG C/min speed is warming up to 250 DEG C, is kept for 5 minutes；Injector temperature is 250 DEG C, and detector temperature is 250 DEG C, is entered
Sample amount 10uL, theoretical cam curve is based on dendrobine, not less than 10000.
It is prepared by 2.4 standard curves
Precision measure reference substance solution 0.2,0.5,0.8,1.0,2.0,4.0m L be respectively placed in 10m L measuring bottles, add first
Alcohol shakes up to scale.Determined by above-mentioned chromatographic condition.Using integrating peak areas as ordinate, reference substance sample size enters for abscissa
Row linear regression, regression equation are A=0.7522C -0.0252, r=0.9997, show dendrobine in 0.10-2.00ug models
There is good linear relationship in enclosing.
2.5 precision test
Precision draws dendrobine reference substance solution l0ul, and continuous 6 sample introductions record peak area, and the RSD of dendrobine peak area is
1.58%, show that instrument precision is good.
2.6 replica test
6 parts of need testing solutions 1 and need testing solution 2 are prepared as stated above, are measured by above-mentioned chromatographic condition.Dendrobine peak
The RSD of area is respectively 2.05% and 2.06%.
2.7 stability test
Take same need testing solution 1, place at room temperature, respectively at 0,2,4,6,8, l2 hour sample introductions, record dendrobine
Peak area, RSD 2.88%.As a result show, need testing solution 1 is stable in 12 hours.
Take same need testing solution 2, place at room temperature, respectively at 0,2,4,6,8, l2 hour sample introductions, record stone
Dry measure used in former times alkali peak area, RSD 2.89%.As a result show, need testing solution 2 is stable in 12 hours.
2.8 recovery test
Take known content 0.46mg/g) 6 parts of dendrobium candidum powder, every part of about 1.65g is accurately weighed.It is accurate to add dendrobine
The mL of reference substance mother liquor 2, prepares need testing solution 1 and need testing solution 2 as stated above, is surveyed under above-mentioned chromatographic condition
It is fixed, calculate the rate of recovery.As a result average recovery rate is 97.1% and 96.8%, RSD are respectively 2.5% and 2.6%.
2.9 sample sizes determine
By the preparation method and condition determination of above-mentioned need testing solution 1 and need testing solution 2, it is divided into 6 parts, determines 6 parts of iron sheet stones
The content of dendrobine in the sample of dry measure used in former times 1 and 6 parts of samples of dendrobium candidum 2, result is recorded, results averaged, the results are shown in Table 1.
As seen from table, the inventive method cultivates obtained dendrobium candidum, and dendrobine contains in the dendrobium candidum finally harvested
Amount is higher.The quality better of dendrobium candidum.
Compared with prior art, during the inventive method cultivation of dendrobium officinale, the dendrobium candidum quality better of final output, effectively
Component content is high, particularly the height of the content of dendrobine.
Embodiment
Embodiment 1.
A kind of pseudo-wild cultivating method of the high dendrobium candidum of stem of noble dendrobium alkali content, specifically includes following steps：
（1）Seed is sprouted：Seeds of Dendrobium Candidum is taken, after cleaning, 70-80% ethanol disinfection 10min is used on superclean bench
Afterwards, then with aseptic water washing 4 times, it is placed on the filter paper of sterilizing after blotting, is inoculated on germination medium, be 20-25 in daytime temperature
DEG C, under the conditions of 18-20 DEG C of night temperature, light culture, until seed is sprouted；
（2）Seedling culture：Treat seed sprout, grow into 3-4cm it is high when, when having 3-5 bar adventitious roots, remove, be positioned in greenhouse
Culture 13 days, then outdoor continue culture 8 days, obtain dendrobium candidum seedling；
（3）Paste tree planting：Plantation selection is struck off the deep barks of 3-5cm by 30-40cm in the tree on spring, fir, and the root and stem of certain plants applies
Slurry mixture, then by 5-10 strains be 1 clump fill at broken skin or tree lobe ditch at be adjacent to bark, then carry out one layer of straw, use bamboo
The thin bamboo strip bundlees, and during binding, only ties up root system, exposes stem foot；
（4）Weeding：Weeding every year 3 times, carried out in the annual Spring Equinox to Clear and Bright, remove weeds and dry branches and fallen leaves；
（5）Top dressing：First time top dressing sprays Liquid Fertilizer 6 days before Clear and Bright, is pasted with fertilizer in root within second 6 days after the Beginning of Winter；
Described fertilizer press by 45% mud 55kg, mashed potatoes 10kg, peptone 0.3kg, agar 0.3kg, vitamin B1 0.3kg,
Malt 0.3kg and tangerine peel powder 5kg is mixed, and is produced；
（6）Watering：After cultivation in 6 days, 3 water are poured daily, bark humid control is 80%；
（7）Harvesting：After cultivation 1 year, harvesting.
The germination medium is by sucrose 5kg, sweet potato mash 15kg, soybean residue 10kg, Polygonum multiflower knotweed 10kg, radix bupleuri 15kg, Huang
Even 10kg, subprostrate sophora 10kg, bighead atractylodes rhizome 15kg and Chinese ephedra 15kg are made as follows：It is Polygonum multiflower knotweed, radix bupleuri, the coptis, subprostrate sophora, white
After art and Chinese ephedra add the decoctings of 11 times of amounts to boil 2.5h, filtering, filter residue is taken, dry, pulverize into coarse granule, it is coarse granule and sucrose, red
Mashed potatoes, soybean residue mix, and produce.
The slurry mixture is by wall fern 25kg, 65% mud 45kg, chicken manure 25kg, lotus leaf slag 10kg, mulberry leaf slag
10kg, red sage root slag 10kg and motherwort slag 10kg are mixed, and are produced.
Described lotus leaf slag；It is to add 6 times of amount decoctings to boil 45min in lotus leaf, decoction liquor filtering, takes filter residue, produce；Described
Mulberry leaf slag；It is to add 5 times of amount decoctings to boil 45min in mulberry leaf, decoction liquor filtering, takes filter residue, produce；Described red sage root slag；It is the red sage root
In plus 5 times of amount decoctings boil 45min, decoction liquor filtering, take filter residue, produce；Described motherwort slag；It is to add 6 times of amounts in motherwort
45min is boiled in decocting, decoction liquor filtering, is taken filter residue, is produced.
Described Liquid Fertilizer is mixed by cow dung 15kg, urea 0.3kg and water 95kg, is produced.
Embodiment 2.
A kind of pseudo-wild cultivating method of the high dendrobium candidum of stem of noble dendrobium alkali content, specifically includes following steps：
（1）Seed is sprouted：Seeds of Dendrobium Candidum is taken, after cleaning, after 80% ethanol disinfection 15min is used on superclean bench,
Aseptic water washing is used again 4 times, is placed on the filter paper of sterilizing after blotting, is inoculated on germination medium, be 20-25 DEG C in daytime temperature,
Under the conditions of 18-20 DEG C of night temperature, light culture, until seed is sprouted；
（2）Seedling culture：Treat seed sprout, grow into 3-4cm it is high when, when having 3-5 bar adventitious roots, remove, be positioned in greenhouse
Culture 15 days, then outdoor continue culture 9 days, obtain dendrobium candidum seedling；
（3）Paste tree planting：Plantation selection is struck off the deep barks of 3-5cm by 30-40cm in the tree on spring, fir, and the root and stem of certain plants applies
Slurry mixture, then by 5-10 strains be 1 clump fill at broken skin or tree lobe ditch at be adjacent to bark, then carry out one layer of straw, use bamboo
The thin bamboo strip bundlees, and during binding, only ties up root system, exposes stem foot；
（4）Weeding：Weeding every year 3 times, carried out in the annual Spring Equinox to Clear and Bright, remove weeds and dry branches and fallen leaves；
（5）Top dressing：First time top dressing sprays Liquid Fertilizer 7 days before Clear and Bright, is pasted with fertilizer in root within second 7 days after the Beginning of Winter；
Described fertilizer press by 50% mud 60kg, mashed potatoes 15kg, peptone 0.5kg, agar 0.5kg, vitamin B1 0.5kg,
Malt 0.5kg and tangerine peel powder 10kg is mixed, and is produced；
（6）Watering：After cultivation in 7 days, 3 water are poured daily, bark humid control is 85%；
（7）Harvesting：Cultivation can harvest after 2 years.
The germination medium hands over 13kg, radix bupleuri 18kg, the coptis by sucrose 5kg, sweet potato mash 17kg, soybean residue 13kg, night
13kg, subprostrate sophora 13kg, bighead atractylodes rhizome 18kg and Chinese ephedra 18kg are made as follows：Polygonum multiflower knotweed, radix bupleuri, the coptis, subprostrate sophora, the bighead atractylodes rhizome
After 3h is boiled in the decoctings measured with Chinese ephedra plus 12 times, filtering, take filter residue, dry, pulverize into coarse granule, coarse granule and sucrose, sweet potato mash,
Soybean residue mixes, and produces.
The slurry mixture is by wall fern 30kg, 70% mud 50kg, chicken manure 30kg, lotus leaf slag 15kg, mulberry leaf slag
15kg, red sage root slag 15kg and motherwort slag 15kg are mixed, and are produced.
Described lotus leaf slag；It is to add 6 times of amount decoctings to boil 50min in lotus leaf, decoction liquor filtering, takes filter residue, produce；Described
Mulberry leaf slag；It is to add 6 times of amount decoctings to boil 50min in mulberry leaf, decoction liquor filtering, takes filter residue, produce；Described red sage root slag；It is the red sage root
In plus 6 times of amount decoctings boil 50min, decoction liquor filtering, take filter residue, produce；Described motherwort slag；It is to add 6 times of amounts in motherwort
50min is boiled in decocting, decoction liquor filtering, is taken filter residue, is produced.
Described Liquid Fertilizer is mixed by cow dung 20kg, urea 0.5kg and water 100kg, is produced.
Embodiment 3.
A kind of pseudo-wild cultivating method of the high dendrobium candidum of stem of noble dendrobium alkali content, specifically includes following steps：
（1）Seed is sprouted：Seeds of Dendrobium Candidum is taken, after cleaning, after 70% ethanol disinfection 5min is used on superclean bench,
Aseptic water washing is used again 3 times, is placed on the filter paper of sterilizing after blotting, is inoculated on germination medium, be 20-25 DEG C in daytime temperature,
Under the conditions of 18-20 DEG C of night temperature, light culture, until seed is sprouted；
（2）Seedling culture：Treat seed sprout, grow into 3-4cm it is high when, when having 3-5 bar adventitious roots, remove, be positioned in greenhouse
Culture 10 days, then outdoor continue culture 7 days, obtain dendrobium candidum seedling；
（3）Paste tree planting：Plantation selection is struck off the deep barks of 3-5cm by 30-40cm in the tree on spring, fir, and the root and stem of certain plants applies
Slurry mixture, then by 5-10 strains be 1 clump fill at broken skin or tree lobe ditch at be adjacent to bark, then carry out one layer of straw, use bamboo
The thin bamboo strip bundlees, and during binding, only ties up root system, exposes stem foot；
（4）Weeding：Weeding every year 2 times, carried out in the annual Spring Equinox to Clear and Bright, remove weeds and dry branches and fallen leaves；
（5）Top dressing：First time top dressing sprays Liquid Fertilizer 5 days before Clear and Bright, is pasted with fertilizer in root within second 5 days after the Beginning of Winter；
Described fertilizer is pressed by 40% mud 50kg, mashed potatoes 5kg, peptone 0.1kg, agar 0.1kg, vitamin B1 0.1kg, wheat
Bud 0.1kg and tangerine peel powder 1kg is mixed, and is produced；
（6）Watering：After cultivation in 5 days, 2 water are poured daily, bark humid control is 75%；
（7）Harvesting：Cultivation can harvest after 1 year.
The germination medium hands over 13kg, radix bupleuri 18kg, the coptis by sucrose 5kg, sweet potato mash 17kg, soybean residue 13kg, night
13kg, subprostrate sophora 13kg, bighead atractylodes rhizome 18kg and Chinese ephedra 18kg are made as follows：Polygonum multiflower knotweed, radix bupleuri, the coptis, subprostrate sophora, the bighead atractylodes rhizome
After 3h is boiled in the decoctings measured with Chinese ephedra plus 12 times, filtering, take filter residue, dry, pulverize into coarse granule, coarse granule and sucrose, sweet potato mash,
Soybean residue mixes, and produces.
The slurry mixture is by wall fern 20kg, 60% mud 40kg, chicken manure 20kg, lotus leaf slag 5kg, mulberry leaf slag
5kg, red sage root slag 5kg and motherwort slag 5kg are mixed, and are produced.
Described lotus leaf slag；It is to add 5 times of amount decoctings to boil 40min in lotus leaf, decoction liquor filtering, takes filter residue, produce；Described
Mulberry leaf slag；It is to add 5 times of amount decoctings to boil 40min in mulberry leaf, decoction liquor filtering, takes filter residue, produce；Described red sage root slag；It is the red sage root
In plus 5 times of amount decoctings boil 40min, decoction liquor filtering, take filter residue, produce；Described motherwort slag；It is to add 5 times of amounts in motherwort
40min is boiled in decocting, decoction liquor filtering, is taken filter residue, is produced.
Described Liquid Fertilizer is mixed by cow dung 10kg, urea 0.1kg and water 90kg, is produced.
Claims (10)
- A kind of 1. pseudo-wild cultivating method of the high dendrobium candidum of stem of noble dendrobium alkali content, it is characterised in that：Comprise the following steps：（1）Seed is sprouted：Seeds of Dendrobium Candidum is taken, after cleaning, 70-80% ethanol disinfection 5- is used on superclean bench After 15min, then with aseptic water washing 3-4 times, it is placed on the filter paper of sterilizing after blotting, is inoculated on germination medium, in daytime temperature For 20-25 DEG C, under the conditions of 18-20 DEG C of night temperature, light culture, until seed is sprouted；（2）Seedling culture：Treat seed sprout, grow into 3-4cm it is high when, when having 3-5 bar adventitious roots, remove, be positioned in greenhouse Culture 10-15 days, then outdoor continue culture 7-9 days, obtain dendrobium candidum seedling；（3）Paste tree planting：Plantation selection is struck off the deep barks of 3-5cm by 30-40cm in the tree on spring, fir, and the root and stem of certain plants applies Slurry mixture, then by 5-10 strains be 1 clump fill at broken skin or tree lobe ditch at be adjacent to bark, then carry out one layer of straw, use bamboo The thin bamboo strip bundlees, and during binding, only ties up root system, exposes stem foot；（4）Weeding：Weeding every year 2-3 times, carried out in the annual Spring Equinox to Clear and Bright, remove weeds and dry branches and fallen leaves；（5）Top dressing：First time top dressing sprays Liquid Fertilizer 5-7 days before Clear and Bright, is pasted with fertilizer within second 5-7 days after the Beginning of Winter Root；（6）Watering：After cultivation in 5-7 days, 2-3 water is poured daily, bark humid control is in 75-85%；（7）Harvesting：It can be harvested after cultivation 1-2.
- 2. the pseudo-wild cultivating method of the high dendrobium candidum of stem of noble dendrobium alkali content as claimed in claim 1, it is characterised in that：It is described Step（1）In, the main sucrose 3-6 part, sweet potato mash 10-20 parts, soybean residue 5- by calculating by weight of the germination medium 15 parts, Polygonum multiflower knotweed 5-15 parts, radix bupleuri 10-20 parts, coptis 5-15 parts, subprostrate sophora 5-15 parts, bighead atractylodes rhizome 10-20 parts and Chinese ephedra 10-20 Part is made as follows：After Polygonum multiflower knotweed, radix bupleuri, the coptis, subprostrate sophora, the bighead atractylodes rhizome and Chinese ephedra add the 10-12 times of decocting measured to boil 2-3h, Filtering, takes filter residue, dry, pulverize into coarse granule, and coarse granule is mixed with sucrose, sweet potato mash, soybean residue, produced.
- 3. the pseudo-wild cultivating method of the high dendrobium candidum of stem of noble dendrobium alkali content as claimed in claim 2, it is characterised in that：It is described Main 5 parts of sucrose, 15 parts of sweet potato mash, 10 parts of soybean residue, 10 parts of Polygonum multiflower knotweed, the radix bupleuri 15 by calculating by weight of germination medium 15 parts of part, 10 parts of the coptis, 10 parts of subprostrate sophora, 15 parts of the bighead atractylodes rhizome and Chinese ephedra are made as follows：Polygonum multiflower knotweed, radix bupleuri, the coptis, mountain beans After root, the bighead atractylodes rhizome and Chinese ephedra add the decocting of 11 times of amounts to boil 2.5h, filtering, filter residue is taken, dry, pulverize into coarse granule, coarse granule and sugarcane Sugar, sweet potato mash, soybean residue mix, and produce.
- 4. the pseudo-wild cultivating method of the high dendrobium candidum of stem of noble dendrobium alkali content as claimed in claim 1, it is characterised in that：Step （3）In, the slurry mixture calculates by weight, mainly by wall fern 20-30 parts, 60-70% mud 40-50 parts, chicken manure 20-30 parts, lotus leaf slag 5-15 parts, mulberry leaf slag 5-15 parts, red sage root slag 5-15 parts and motherwort slag 5-15 parts mix, and produce.
- 5. the pseudo-wild cultivating method of the high dendrobium candidum of stem of noble dendrobium alkali content as claimed in claim 4, it is characterised in that：It is described Slurry mixture calculates by weight, mainly by 25 parts of the wall fern, 60-70% 10 parts of 25 parts of 45 parts of mud, chicken manure, lotus leaf slag, 10 parts of 10 parts of mulberry leaf slag, 10 parts of red sage root slag and motherwort slag mixings, are produced.
- 6. the pseudo-wild cultivating method of the high dendrobium candidum of stem of noble dendrobium alkali content as described in claim 4 or 5, it is characterised in that： Described lotus leaf slag；It is to add 5-6 times to measure decocting in lotus leaf to boil 40-50min, decoction liquor filtering, takes filter residue, produce；Described mulberry Leaf slag；It is to add 5-6 times to measure decocting in mulberry leaf to boil 40-50min, decoction liquor filtering, takes filter residue, produce；Described red sage root slag；It is red In ginseng plus 5-6 times is measured decocting and boils 40-50min, decoction liquor filtering, is taken filter residue, is produced；Described motherwort slag；It is in motherwort Add 5-6 times to measure decocting and boil 40-50min, decoction liquor filtering, take filter residue, produce.
- 7. the pseudo-wild cultivating method of the high dendrobium candidum of stem of noble dendrobium alkali content as claimed in claim 1, it is characterised in that：Step （5）In, described Liquid Fertilizer calculates by weight, is mainly mixed by cow dung 10-20 parts, urea 0.1-0.5 parts and water 90-100 parts It is even, produce.
- 8. the pseudo-wild cultivating method of the high dendrobium candidum of stem of noble dendrobium alkali content as claimed in claim 7, it is characterised in that：It is described Liquid Fertilizer calculate by weight, mainly by 15 parts of cow dung, 0.3 part of urea and the mixing of 95 parts of water, produce.
- 9. the pseudo-wild cultivating method of the high dendrobium candidum of stem of noble dendrobium alkali content as claimed in claim 1, it is characterised in that：Step （5）In, described fertilizer calculates by weight, mainly by 40-50% mud 50-60 parts, mashed potatoes 5-15 parts, peptone 0.1-0.5 parts, agar 0.1-0.5 parts, vitamin B1 0.1-0.5 parts, malt 0.1-0.5 parts and tangerine peel powder 1-10 parts mix, i.e., .
- 10. the pseudo-wild cultivating method of the high dendrobium candidum of stem of noble dendrobium alkali content as claimed in claim 9, it is characterised in that：Institute The fertilizer stated calculates by weight, mainly by 40-50% 0.3 part of 0.3 part of 10 parts of 55 parts of mud, mashed potatoes, peptone, agar, 5 parts of 0.3 part of vitamin B1,0.3 part of malt and tangerine peel powder mixings, are produced.
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