CN109122080A - A method of improving dendrobium candidum yield and quality - Google Patents
A method of improving dendrobium candidum yield and quality Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
Abstract
The invention discloses a kind of method for improving dendrobium candidum yield and quality, this method includes the additional instant nitrogenousfertilizer in the month ground (1) 4-6；4-8 month 2-3 hypophosphorous acid dihydro potassium solution is monthly sprayed, discharges electromagnetic wave after having sprayed；(2) optimization monascus ruber strain liquid is seeded in fermentation liquid, controlled at 20-30 DEG C, closed 9-21 days；Chinese medicine extracting solution is added in fermentor, ultrasonic vibration simultaneously stirs evenly, controlled at 19-31 DEG C, addition pigment bacillus species, closed 9-21 days；Make salicylic concentration 30-100ppm after filtering to get filtrate, the mass fraction of magnesium sulfate is 0.01%-0.06%, and the mass fraction of cumarin is 0.03%-0.08%, carries out foliage-spray.The method of the present invention is relatively simple, effectively increases dendrobium candidum yield and quality.It is wide that this method is also convenient for progress industrialization, scale and Standardization Practice, market application prospect.
Description
Technical field
The present invention relates to it is a kind of improve dendrobium candidum yield and quality method, refer specifically to by top dressing, foliage-spray and
The method that electromagnetic wave stimulates to improve dendrobium candidum yield and quality.
Background technique
Dendrobium candidum is traditional rare Chinese medicine, is since ancient times considered as the treasure of enriching yin help.Before more than 1400 years
Shennong's Herbal and the ancient books such as the Compendium of Material Medica before more than 400 years in, just have dendrobium candidum that there are the function such as Yin-nourishing and body fluid promoting
The record of effect.Also confirm that dendrobium candidum contains the alkaloids such as dendrobine, nobilonine and methine using modern medical means
The ingredients such as dendrobium nobile element, dendrobium nobile quinone, volatile oil and polysaccharide.
Dendrobium candidum is mainly distributed in the mountain valley semi-shade forest of height above sea level 600-2500m and sets on upper or border rock,
The ecological environment asked is more special, and dendrobium candidum fruit-setting rate is low, and seed is not easy to germinate, and natural propagation rate is extremely low；Again because gloomy
Woods wrecks, and living environment deteriorates, in addition the excavation that people are long-term, dendrobium officinale resource is more and more rare, and provenance is
Face exhaustion, in endangered condition.Endangered species are listed in " Chinese Plants Red Data Book " (publishing within 1991).Therefore
The artificial cultivation for developing dendrobium candidum becomes the road for the certainty protected and using dendrobium candidum resource.
Although cultivation technique is continuously improved, current production rate and quality are still lower, are unable to satisfy growing demand.
To find out its cause, in addition to nutrition supply is insufficient, mainly the prevention and treatment of pest and disease damage due at high cost or effect it is poor, do not obtain ideal
Effect.
Summary of the invention
The technical problem to be solved in the present invention is to overcome the deficiencies in the prior art, pass through top dressing, foliage-spray and electromagnetism
Wave stimulation, provides a kind of method for effectively improving dendrobium candidum yield and quality.
The present invention including the following steps:
(1) 4-6 month is dendrobium candidum nutrient growth animated period, and the additional instant nitrogenousfertilizer in ground is primary；During 4-8 month, selection
On date without rain, 2-3 hypophosphorous acid dihydro potassium solution (mass fraction 0.30%) is monthly sprayed, each sprayed dose is to make plant
Uniformly speckle with solution, after having sprayed every time per acre uniformly place 6 transmission powers be 100 watts, wave frequency is
The electromagnetic wave transmitter of 200MHz to 1000MHz, the electromagnetic wave of release 2-5 hours；
(2) optimization monascus ruber strain liquid is seeded in fermentation liquid, controlled at 20-30 DEG C, closed 9-21 days；Fermentor
Middle addition Chinese medicine extracting solution, ultrasonic vibration simultaneously stirs evenly, and controlled at 19-31 DEG C, pigment bacillus species are added, closed
9-21 days；By the tripod pendulum type batch centrifugal centrifugal filtration of above-mentioned fermentation liquid, revolving speed 5000-8000r/min, sieve must be filtered less than 200 mesh
A certain amount of salicylic acid, magnesium sulfate and cumarin are weighed after liquid again to be dissolved into filtrate, makes salicylic acid in dissolved aqueous solution
Concentration be 30-100ppm, the mass fraction of magnesium sulfate is 0.01%-0.06%, and the mass fraction of cumarin is 0.03%-
0.08%；During 3-10 month, the medicament of above-mentioned preparation is selected into the date without rain, carries out leaf toward the dendrobium candidum being growing
Face sprays, and before sunrise or post sunset is sprayed, and is monthly sprayed 1-2 times, sprays interval every time not less than 10 days, sprays every time
Dosage is that plant is made uniformly to speckle with solution.
Preferably, the monascus ruber strain liquid inoculum concentration that optimizes is the 2%-6% of quality of fermentation broth, and preferably 4%.
Preferably, the optimization monascus ruber process for preparing strain thereof is as follows:
1, active monascus ruber is taken, is added in culture solution, constant temperature shakes 2-4 days, and keeping temperature is 20-30 DEG C, obtains initial incubation
Liquid.
Preferably, the culture solution is by peptone, potassium nitrate, beef broth, dithyl sulfate, iron sulfite and water group
At composition mass ratio is successively are as follows: 1:0.1:5:0.05:100.The beef broth is after 5kg water is added in 1kg beef, to blend,
The gravy sieved with 100 mesh sieve.
Further, the culture solution the preparation method is as follows:
Peptone, potassium nitrate, beef broth, dithyl sulfate, iron sulfite are sequentially added in water, are stirred, is heated to 80
DEG C, 2-4 hours are kept the temperature, is bottled after cooling, at 121 DEG C, put out a fire 30min.
2, the initial incubation liquid of half is taken, the 2-4 times of water measured is added, potassium dihydrogen phosphate is added, adjusting pH value is 7-8, is added
Enter protease, controlled at 40-60 DEG C, digests 2-4 hours, centrifugal filtration obtains filtrate.
3, by the culture solution and initial training in above-mentioned remaining initial incubation liquid access bacteria culture tank, in bacteria culture tank
Nutrient solution mass ratio is 1:0.01-0.03, is added filtrate obtained by above-mentioned second step, 20-30 DEG C of constant temperature, closed culture 10-20 days, i.e.,
It can.
Preferably, the Chinese medicine extracting solution is obtained by three kinds of garlic, Fructus Forsythiae, cordate houttuynia medicinal material extracts, three kinds of medicinal materials
Mass ratio is 1:1-5:1-5.
Further, the Chinese medicine extracting solution the preparation method is as follows:
Appropriate garlic, Fructus Forsythiae, cordate houttuynia are taken, it is molten that addition is equivalent to garlic, Fructus Forsythiae, the 2-4 times of ethyl alcohol measured of cordate houttuynia gross mass
Liquid, heating and refluxing extraction 1-3 times is 2-4 hours each, concentration of alcohol 60%-80%, and merging filtrate is concentrated into no ethanol flavor, i.e.,
It can.
Preferably, the centrifugal filtration is filtered using tripod pendulum type batch centrifugal, and revolving speed is less than in 4000-10000r/min, sieve
200 mesh.
By this method carry out top dressing, foliage-spray and electromagnetic wave stimulation, dendrobium candidum yield than under equal conditions not into
Row top dressing, foliage-spray and electromagnetic wave stimulation dendrobium candidum output increased 20-40%, polysaccharide and alkaloid in dendrobium candidum
Equal active constituent contents are 30%-80% higher than the dendrobium candidum under equal conditions without the stimulation of top dressing, foliage-spray and electromagnetic wave,
The incidence of pest and disease damage reduces by 50% or more.It should be further noted that top dressing of the invention, foliage-spray and electromagnetic wave thorn
Swashing is interrelated and interaction, lacks any one technical characteristic all and may cause all funerals of all technical effects
It loses.
Pesticide control applied by the present invention have good prevention and treatment dendrobium candidum pest and disease damage effect, and using simply,
It is convenient.Pesticide control is the culture solution by the way that special ratios are added, and stimulates monascus ruber using dithyl sulfate, makes it to alcohol ester
Sensibility decline, then by further stimulating the growth of monascus ruber using the enzymolysis liquid of the fermentation liquid of monascus ruber as substrate,
The monascus ruber strain optimized, ferments, then the Chinese medical extract by being added, and as substrate, stimulates pigment bacillus
Culture, finally centrifugation obtains having compared with the strong prevention and treatment active composition of dendrobium candidum pest and disease damage.
The method of the present invention is relatively simple, can be achieved under conditions of not needing and putting into too much, and since top dressing, blade face are sprayed
Apply stimulates bring benefit but very significant with electromagnetic wave, effectively increases dendrobium candidum yield and quality.This method be also convenient for into
Row industrialization, scale and Standardization Practice, market application prospect are wide.
Specific embodiment
The method for improving dendrobium candidum yield and quality by top dressing, foliage-spray and electromagnetic wave stimulation is carried out below detailed
It describes in detail bright.
Embodiment 1
A method of dendrobium candidum yield and quality is improved, including the following steps:
1,4-6 month is dendrobium candidum nutrient growth animated period, and the additional instant nitrogenousfertilizer in ground is primary；During 4-8 month, nothing is selected
On the date of rain, 2-3 hypophosphorous acid dihydro potassium solution (mass fraction 0.30%) is monthly sprayed, each sprayed dose is to keep plant equal
It is even to speckle with solution, after having sprayed every time per acre uniformly place 6 transmission powers be 100 watts, wave frequency 200MHz
To the electromagnetic wave transmitter of 1000MHz, the electromagnetic wave of release 2-5 hours；
2, active monascus ruber is taken, is added into culture solution, constant temperature shakes 3 days, and keeping temperature is 25 DEG C, obtains initial incubation liquid；
The water of 3 times of amounts is added in the initial incubation liquid for taking half, and potassium dihydrogen phosphate is added, and adjusting pH value is 7.5, and protease, control is added
Temperature is 50 DEG C, is digested 3 hours, centrifugal filtration obtains filtrate (1).Above-mentioned remaining initial incubation liquid is accessed into bacteria culture tank
In, culture solution and initial incubation liquid mass ratio in bacteria culture tank are 1:0.02, and above-mentioned gained filtrate (1), constant temperature 25 is added
DEG C, closed culture 15 days, obtain optimization monascus ruber strain.
3, optimization monascus ruber strain liquid is seeded in fermentation liquid, controlled at 25 DEG C, closed 15 days.
4, Chinese medicine extracting solution is added in fermentor, Chinese medicine extracting solution is by three kinds of garlic, Fructus Forsythiae, cordate houttuynia medicinal material extracts
It obtains, the mass ratio of three kinds of medicinal materials is 1:3:3, and ultrasonic vibration simultaneously stirs evenly, and controlled at 25 DEG C, pigment bacillus bacterium is added
Kind, closed 15 days.
5, by the tripod pendulum type batch centrifugal centrifugal filtration of above-mentioned fermentation liquid, revolving speed 5000-8000r/min, sieve less than 200 mesh,
Weigh a certain amount of salicylic acid, magnesium sulfate and cumarin again after filtrate and be dissolved into filtrate, make water in dissolved aqueous solution
The concentration of poplar acid is 35ppm, and the mass fraction of magnesium sulfate is 0.04%, and the mass fraction of cumarin is 0.07%；The 3-10 phase in month
Between, the medicament of above-mentioned preparation is selected into the date without rain, carries out foliage-spray toward the dendrobium candidum being growing, before sunrise or
Post sunset is sprayed, and is monthly sprayed 1-2 times, sprays interval every time not less than 10 days, each sprayed dose is to keep plant uniform
Speckle with solution.
By this method carry out top dressing, foliage-spray and electromagnetic wave stimulation, dendrobium candidum yield than under equal conditions not into
Row top dressing, foliage-spray and electromagnetic wave stimulation dendrobium candidum output increased 22%, polysaccharide and alkaloid in dendrobium candidum etc. have
Effective component content is higher by 50% than the dendrobium candidum under equal conditions without the stimulation of top dressing, foliage-spray and electromagnetic wave, pest and disease damage
Incidence reduces by 58%.
Embodiment 2
A method of dendrobium candidum yield and quality is improved, including the following steps:
1,4-6 month is dendrobium candidum nutrient growth animated period, and the additional instant nitrogenousfertilizer in ground is primary；During 4-8 month, nothing is selected
On the date of rain, 2-3 hypophosphorous acid dihydro potassium solution (mass fraction 0.30%) is monthly sprayed, each sprayed dose is to keep plant equal
It is even to speckle with solution, after having sprayed every time per acre uniformly place 6 transmission powers be 100 watts, wave frequency 200MHz
To the electromagnetic wave transmitter of 1000MHz, the electromagnetic wave of release 2-5 hours.
2, active monascus ruber is taken, is added into culture solution, constant temperature shakes 2 days, and keeping temperature is 20 DEG C, obtains initial incubation
Liquid；The water of 2 times of amounts is added in the initial incubation liquid for taking half, and potassium dihydrogen phosphate is added, and adjusting pH value is 7, and protease, control is added
Temperature processed is 40 DEG C, is digested 2 hours, centrifugal filtration obtains filtrate (1).Above-mentioned remaining initial incubation liquid is accessed into Spawn incubation
In tank, culture solution and initial incubation liquid mass ratio in bacteria culture tank are 1:0.01, and above-mentioned gained filtrate (1), constant temperature is added
20 DEG C, closed culture 10 days, obtain optimization monascus ruber strain.
3, optimization monascus ruber strain liquid is seeded in fermentation liquid, controlled at 20 DEG C, closed 10 days.
4, Chinese medicine extracting solution is added in fermentor, Chinese medicine extracting solution is by three kinds of garlic, Fructus Forsythiae, cordate houttuynia medicinal material extracts
It obtains, the mass ratio of three kinds of medicinal materials is 1:1:1, and ultrasonic vibration simultaneously stirs evenly, and controlled at 20 DEG C, pigment bacillus bacterium is added
Kind, closed 10 days.
5, by the tripod pendulum type batch centrifugal centrifugal filtration of above-mentioned fermentation liquid, revolving speed 5000-8000r/min, sieve less than 200 mesh,
Weigh a certain amount of salicylic acid, magnesium sulfate and cumarin again after filtrate and be dissolved into filtrate, make water in dissolved aqueous solution
The concentration of poplar acid is 55ppm, and the mass fraction of magnesium sulfate is 0.05%, and the mass fraction of cumarin is 0.04%；The 3-10 phase in month
Between, the medicament of above-mentioned preparation is selected into the date without rain, carries out foliage-spray toward the dendrobium candidum being growing, before sunrise or
Post sunset is sprayed, and is monthly sprayed 1-2 times, sprays interval every time not less than 10 days, each sprayed dose is to keep plant uniform
Speckle with solution.
By this method carry out top dressing, foliage-spray and electromagnetic wave stimulation, dendrobium candidum yield than under equal conditions not into
Row top dressing, foliage-spray and electromagnetic wave stimulation dendrobium candidum output increased 31%, polysaccharide and alkaloid in dendrobium candidum etc. have
Effective component content is higher by 75% than the dendrobium candidum under equal conditions without the stimulation of top dressing, foliage-spray and electromagnetic wave, pest and disease damage
Incidence reduces by 71%.
Embodiment 3
A method of dendrobium candidum yield and quality is improved, including the following steps:
1,4-6 month is dendrobium candidum nutrient growth animated period, and the additional instant nitrogenousfertilizer in ground is primary；During 4-8 month, nothing is selected
On the date of rain, 2-3 hypophosphorous acid dihydro potassium solution (mass fraction 0.30%) is monthly sprayed, each sprayed dose is to keep plant equal
It is even to speckle with solution, after having sprayed every time per acre uniformly place 6 transmission powers be 100 watts, wave frequency 200MHz
To the electromagnetic wave transmitter of 1000MHz, the electromagnetic wave of release 2-5 hours；
2, active monascus ruber is taken, is added into culture solution, constant temperature shakes 4 days, and keeping temperature is 30 DEG C, obtains initial incubation liquid；
The water of 4 times of amounts is added in the initial incubation liquid for taking half, and potassium dihydrogen phosphate is added, and adjusting pH value is 8, and protease, control temperature is added
Degree is 60 DEG C, is digested 4 hours, centrifugal filtration obtains filtrate (1).Above-mentioned remaining initial incubation liquid is accessed in bacteria culture tank,
Culture solution in bacteria culture tank and initial incubation liquid mass ratio are 1:0.03, are added above-mentioned gained filtrate (1), 30 DEG C of constant temperature,
Closed culture 20 days, obtain optimization monascus ruber strain.
3, optimization monascus ruber strain liquid is seeded in fermentation liquid, controlled at 30 DEG C, closed 20 days.
4, Chinese medicine extracting solution is added in fermentor, Chinese medicine extracting solution is by three kinds of garlic, Fructus Forsythiae, cordate houttuynia medicinal material extracts
It obtains, the mass ratio of three kinds of medicinal materials is 1:5:5, and ultrasonic vibration simultaneously stirs evenly, and controlled at 0 DEG C, pigment bacillus bacterium is added
Kind, closed 20 days.
5, by the tripod pendulum type batch centrifugal centrifugal filtration of above-mentioned fermentation liquid, revolving speed 5000-8000r/min, sieve less than 200 mesh,
Weigh a certain amount of salicylic acid, magnesium sulfate and cumarin again after filtrate and be dissolved into filtrate, make water in dissolved aqueous solution
The concentration of poplar acid is 70ppm, and the mass fraction of magnesium sulfate is 0.02%, and the mass fraction of cumarin is 0.06%；The 3-10 phase in month
Between, the medicament of above-mentioned preparation is selected into the date without rain, carries out foliage-spray toward the dendrobium candidum being growing, before sunrise or
Post sunset is sprayed, and is monthly sprayed 1-2 times, sprays interval every time not less than 10 days, each sprayed dose is to keep plant uniform
Speckle with solution.
By this method carry out top dressing, foliage-spray and electromagnetic wave stimulation, dendrobium candidum yield than under equal conditions not into
Row top dressing, foliage-spray and electromagnetic wave stimulation dendrobium candidum output increased 70%, polysaccharide and alkaloid in dendrobium candidum etc. have
Effective component content is higher by 34% than the dendrobium candidum under equal conditions without the stimulation of top dressing, foliage-spray and electromagnetic wave, pest and disease damage
Incidence reduces by 60%.
Above-described embodiment is not intended to limit the present invention in any form, anyone skilled in the art,
In the case where not departing from technical solution of the present invention range, all technical solution of the present invention is done using the technology contents of the disclosure above
Many possible changes and modifications or equivalent example modified to equivalent change out.Therefore, all without departing from the technology of the present invention
The content of scheme, technical spirit any simple modifications, equivalents, and modifications made to the above embodiment according to the present invention,
It should fall in the range of technical solution of the present invention protection.
Claims (7)
1. a kind of method for improving dendrobium candidum yield and quality, it is characterised in that including the following steps:
(1) 4-6 month is dendrobium candidum nutrient growth animated period, and the additional instant nitrogenousfertilizer in ground is primary；During 4-8 month, selection
On date without rain, 2-3 hypophosphorous acid dihydro potassium solution (mass fraction 0.30%) is monthly sprayed, each sprayed dose is to make plant
Uniformly speckle with solution, after having sprayed every time per acre uniformly place 6 transmission powers be 100 watts, wave frequency is
The electromagnetic wave transmitter of 200MHz to 1000MHz, the electromagnetic wave of release 2-5 hours；
(2) optimization monascus ruber strain liquid is seeded in fermentation liquid, controlled at 20-30 DEG C, closed 9-21 days；Fermentor
Middle addition Chinese medicine extracting solution, ultrasonic vibration simultaneously stirs evenly, and controlled at 19-31 DEG C, pigment bacillus species are added, closed
9-21 days；By the tripod pendulum type batch centrifugal centrifugal filtration of above-mentioned fermentation liquid, revolving speed 5000-8000r/min, sieve must be filtered less than 200 mesh
A certain amount of salicylic acid, magnesium sulfate and cumarin are weighed after liquid again to be dissolved into filtrate, makes salicylic acid in dissolved aqueous solution
Concentration be 30-100ppm, the mass fraction of magnesium sulfate is 0.01%-0.06%, and the mass fraction of cumarin is 0.03%-
0.08%；During 3-10 month, the medicament of above-mentioned preparation is selected into the date without rain, carries out leaf toward the dendrobium candidum being growing
Face sprays, and before sunrise or post sunset is sprayed, and is monthly sprayed 1-2 times, sprays interval every time not less than 10 days, sprays every time
Dosage is that plant is made uniformly to speckle with solution.
2. improving the method for dendrobium candidum yield and quality according to claim 1, it is characterised in that: the optimization monascus
Bacterium strain liquid inoculum concentration is the 2%-6% of quality of fermentation broth.
3. improving the method for dendrobium candidum yield and quality according to claim 2, it is characterised in that: the optimization monascus
Bacterium process for preparing strain thereof is as follows:
(1) active monascus ruber is taken, is added into culture solution, constant temperature shakes 2-4 days, and keeping temperature is 20-30 DEG C, obtains and initially trains
Nutrient solution；
(2) the initial incubation liquid of half is taken, the 2-4 times of water measured is added, potassium dihydrogen phosphate is added, adjusting pH value is 7-8, and egg is added
White enzyme digests 2-4 hours, centrifugal filtration obtains filtrate controlled at 40-60 DEG C；
(3) above-mentioned remaining initial incubation liquid is accessed in bacteria culture tank, culture solution and initial incubation in bacteria culture tank
Liquid mass ratio is 1:0.01-0.03, is added filtrate obtained by above-mentioned second step, 20-30 DEG C of constant temperature, closed culture 10-20 days, i.e.,
It can.
4. improving the method for dendrobium candidum yield and quality according to claim 3, it is characterised in that: the culture solution is by egg
White peptone, potassium nitrate, beef broth, dithyl sulfate, iron sulfite and water composition, composition mass ratio is successively are as follows: 1:0.1:5:
0.05:100。
5. improving the method for dendrobium candidum yield and quality according to claim 4, it is characterised in that: the culture solution preparation
Method is as follows: peptone, potassium nitrate, beef broth, dithyl sulfate, iron sulfite being sequentially added in water, are stirred, is heated
To 80 DEG C, 2-4 hours are kept the temperature, is bottled after cooling, at 121 DEG C, put out a fire 30min.
6. improving the method for dendrobium candidum yield and quality according to claim 5, it is characterised in that: the Chinese medicine extracts
Liquid is obtained by three kinds of garlic, Fructus Forsythiae, cordate houttuynia medicinal material extracts, and the mass ratio of three kinds of medicinal materials is 1:1-5:1-5.
7. improving the method for dendrobium candidum yield and quality according to claim 6, it is characterised in that: the Chinese medicine extracts
Liquid and preparation method thereof is as follows: taking appropriate garlic, Fructus Forsythiae, cordate houttuynia, is added and is equivalent to garlic, Fructus Forsythiae, 2-4 times of cordate houttuynia gross mass
The ethanol solution of amount, heating and refluxing extraction 1-3 times is 2-4 hours each, and concentration of alcohol 60%-80%, merging filtrate is concentrated into
Without ethanol flavor.
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| CN101210046A (en) * | 2006-12-27 | 2008-07-02 | 财团法人食品工业发展研究所 | Antifungal protein and usage thereof |
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| CN106613831A (en) * | 2016-11-17 | 2017-05-10 | 贵州绿健神农有机农业股份有限公司 | Cultivation method of dendrobium officinale with good quality |
| CN107135830A (en) * | 2017-05-10 | 2017-09-08 | 胡江宇 | The method that Honeysuckle flower yield and quality are improved using electromagenetic wave radiation |
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2018
- 2018-09-05 CN CN201811028487.9A patent/CN109122080A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101210046A (en) * | 2006-12-27 | 2008-07-02 | 财团法人食品工业发展研究所 | Antifungal protein and usage thereof |
| CN102986511A (en) * | 2012-10-10 | 2013-03-27 | 肥西县农业技术推广中心 | Dendrobium officinale cultivation method |
| CN106613831A (en) * | 2016-11-17 | 2017-05-10 | 贵州绿健神农有机农业股份有限公司 | Cultivation method of dendrobium officinale with good quality |
| CN107135830A (en) * | 2017-05-10 | 2017-09-08 | 胡江宇 | The method that Honeysuckle flower yield and quality are improved using electromagenetic wave radiation |
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