CN109197078A - A method of improving bletilla striata Tuber yields and quality - Google Patents
A method of improving bletilla striata Tuber yields and quality Download PDFInfo
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- CN109197078A CN109197078A CN201811032816.7A CN201811032816A CN109197078A CN 109197078 A CN109197078 A CN 109197078A CN 201811032816 A CN201811032816 A CN 201811032816A CN 109197078 A CN109197078 A CN 109197078A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/60—Flowers; Ornamental plants
- A01G22/63—Orchids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D5/00—Fertilisers containing magnesium
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
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Abstract
The invention discloses a kind of method for improving bletilla striata Tuber yields and quality, this method includes the additional instant nitrogenousfertilizer in the month ground (1) 4-6；4-8 month 2-3 hypophosphorous acid dihydro potassium solution is monthly sprayed, discharges electromagnetic wave after having sprayed；(2) optimization monascus ruber strain liquid is seeded in fermentation liquid, controlled at 20-30 DEG C, closed 9-21 days；Chinese medicine extracting solution is added in fermentor, ultrasonic vibration simultaneously stirs evenly, controlled at 19-31 DEG C, addition pigment bacillus species, closed 9-21 days；Make salicylic concentration 30-100ppm after filtering to get filtrate, the mass fraction of magnesium sulfate is 0.01%-0.06%, and the mass fraction of cumarin is 0.03%-0.08%, carries out foliage-spray.The method of the present invention is relatively simple, effectively increases bletilla striata Tuber yields and quality.It is wide that this method is also convenient for progress industrialization, scale and Standardization Practice, market application prospect.
Description
Technical field
The present invention relates to it is a kind of improve bletilla striata Tuber yields and quality method, refer specifically to by top dressing, foliage-spray and
The method that electromagnetic wave stimulates to improve bletilla striata Tuber yields and quality.
Background technique
The bletilla striata is that the orchid family bletilla striata belongs to perennial tuberous plant, flower aubergine or pink, and alias is even and grass, purple are blue, white
Root, Pseudobulbus Bletillae (Rhizoma Bletillae) and horn seven etc. have very high medical value and ornamental value.Bletilla striata dry tuber has astringing to arrest bleeding, disappears
Swollen myogenic and other effects controls wound hemorrhage, scald outside and treats sore, inside controls haematemesis, tuberculosis, hemoptysis, chronic gastric ulcer and tumour etc..
Modern medicine proves that the bletilla striata also has stronger anti-oxidant and anti-aging effects, can be used for health care, skin care and beauty
Beauty treatment.Therefore, bletilla price continuous rise, supply falls short of demand.But this plant growth rate is slower, generally requires 3 years or more
It could excavate, not have stronger self-reproduction ability, in addition excavating reasons, the bletilla striata such as excessive faces the awkward situation of extinction.Therefore
The artificial cultivation for developing the bletilla striata becomes the road for the certainty protected and using bletilla striata stem tuber resource.
Although cultivation technique is continuously improved, current production rate and quality are still lower, are unable to satisfy growing demand.
To find out its cause, in addition to nutrition supply is insufficient, mainly the prevention and treatment of pest and disease damage due at high cost or effect it is poor, do not obtain ideal
Effect.
Summary of the invention
The technical problem to be solved in the present invention is to overcome the deficiencies in the prior art, pass through top dressing, foliage-spray and electromagnetism
Wave stimulation, provides a kind of method for effectively improving bletilla striata Tuber yields and quality.
The present invention including the following steps:
(1) 4-6 month is bletilla striata stem tuber nutrient growth animated period, and the additional instant nitrogenousfertilizer in ground is primary；During 4-8 month, selection
On date without rain, 2-3 hypophosphorous acid dihydro potassium solution (mass fraction 0.28%) is monthly sprayed, each sprayed dose is to make plant
Uniformly speckle with solution, after having sprayed every time per acre uniformly place 6 transmission powers be 100 watts, wave frequency is
The electromagnetic wave transmitter of 200MHz to 1000MHz, the electromagnetic wave of release 2-5 hours；
(2) optimization monascus ruber strain liquid is seeded in fermentation liquid, controlled at 20-30 DEG C, closed 9-21 days；Fermentor
Middle addition Chinese medicine extracting solution, ultrasonic vibration simultaneously stirs evenly, and controlled at 19-31 DEG C, pigment bacillus species are added, closed
9-21 days；By the tripod pendulum type batch centrifugal centrifugal filtration of above-mentioned fermentation liquid, revolving speed 5000-8000r/min, sieve must be filtered less than 200 mesh
A certain amount of salicylic acid, magnesium sulfate and cumarin are weighed after liquid again to be dissolved into filtrate, makes salicylic acid in dissolved aqueous solution
Concentration be 30-100ppm, the mass fraction of magnesium sulfate is 0.01%-0.06%, and the mass fraction of cumarin is 0.03%-
0.08%；During 3-10 month, the medicament of above-mentioned preparation is selected into the date without rain, carries out blade face spray toward the bletilla striata being growing
It applies, before sunrise or post sunset is sprayed, and is monthly sprayed 1-2 times, and interval is sprayed every time to be not less than 10 days, each sprayed dose
To make plant uniformly speckle with solution.
Preferably, the monascus ruber strain liquid inoculum concentration that optimizes is the 2%-6% of quality of fermentation broth, and preferably 4%.
Preferably, the optimization monascus ruber process for preparing strain thereof is as follows:
1, active monascus ruber is taken, is added in culture solution, constant temperature shakes 2-4 days, and keeping temperature is 20-30 DEG C, obtains initial incubation
Liquid.
Preferably, the culture solution is by peptone, potassium nitrate, beef broth, dithyl sulfate, iron sulfite and water group
At composition mass ratio is successively are as follows: 1:0.1:5:0.05:100.The beef broth is after 5kg water is added in 1kg beef, to blend,
The gravy sieved with 100 mesh sieve.
Further, the culture solution the preparation method is as follows:
Peptone, potassium nitrate, beef broth, dithyl sulfate, iron sulfite are sequentially added in water, are stirred, is heated to 80
DEG C, 2-4 hours are kept the temperature, is bottled after cooling, at 121 DEG C, put out a fire 30min.
2, the initial incubation liquid of half is taken, the 2-4 times of water measured is added, potassium dihydrogen phosphate is added, adjusting pH value is 7-8, is added
Enter protease, controlled at 40-60 DEG C, digests 2-4 hours, centrifugal filtration obtains filtrate.
3, by the culture solution and initial training in above-mentioned remaining initial incubation liquid access bacteria culture tank, in bacteria culture tank
Nutrient solution mass ratio is 1:0.01-0.03, is added filtrate obtained by above-mentioned second step, 20-30 DEG C of constant temperature, closed culture 10-20 days, i.e.,
It can.
Preferably, the Chinese medicine extracting solution is obtained by three kinds of garlic, Fructus Forsythiae, cordate houttuynia medicinal material extracts, three kinds of medicinal materials
Mass ratio is 1:1-5:1-5.
Further, the Chinese medicine extracting solution the preparation method is as follows:
Appropriate garlic, Fructus Forsythiae, cordate houttuynia are taken, it is molten that addition is equivalent to garlic, Fructus Forsythiae, the 2-4 times of ethyl alcohol measured of cordate houttuynia gross mass
Liquid, heating and refluxing extraction 1-3 times is 2-4 hours each, concentration of alcohol 60%-80%, and merging filtrate is concentrated into no ethanol flavor, i.e.,
It can.
Preferably, the centrifugal filtration is filtered using tripod pendulum type batch centrifugal, and revolving speed is less than in 4000-10000r/min, sieve
200 mesh.
By this method carry out top dressing, foliage-spray and electromagnetic wave stimulation, bletilla striata Tuber yields than under equal conditions not into
The bletilla striata Tuber yields of row top dressing, foliage-spray and electromagnetic wave stimulation improve 20-35%, polysaccharide and bibenzyl in bletilla striata stem tuber
Equal active constituent contents are 30%-90% higher than the bletilla striata stem tuber under equal conditions without the stimulation of top dressing, foliage-spray and electromagnetic wave,
The incidence of pest and disease damage reduces by 50% or more.It should be further noted that top dressing of the invention, foliage-spray and electromagnetic wave thorn
Swashing is interrelated and interaction, lacks any one technical characteristic all and may cause all funerals of all technical effects
It loses.
Pesticide control applied by the present invention has good prevention and treatment bletilla striata pest and disease damage effect, and using simply, conveniently.
Pesticide control is the culture solution by the way that special ratios are added, and stimulates monascus ruber using dithyl sulfate, keeps it sensitive to alcohol ester
Property decline, then by using the enzymolysis liquid of the fermentation liquid of monascus ruber as substrate, the further growth of stimulation monascus ruber is obtained
The monascus ruber strain of optimization, ferments, then the Chinese medical extract by being added, and as substrate, stimulates the training of pigment bacillus
It supports, finally centrifugation obtains having compared with the strong prevention and treatment active composition of bletilla striata stem tuber pest and disease damage.
The method of the present invention is relatively simple, can be achieved under conditions of not needing and putting into too much, and since top dressing, blade face are sprayed
Apply stimulates bring benefit but very significant with electromagnetic wave, effectively increases bletilla striata Tuber yields and quality.This method be also convenient for into
Row industrialization, scale and Standardization Practice, market application prospect are wide.
Specific embodiment
The method for improving bletilla striata Tuber yields and quality by top dressing, foliage-spray and electromagnetic wave stimulation is carried out in detail below
It describes in detail bright.
Embodiment 1
A method of bletilla striata Tuber yields and quality are improved, including the following steps:
1,4-6 month is bletilla striata stem tuber nutrient growth animated period, and the additional instant nitrogenousfertilizer in ground is primary；During 4-8 month, nothing is selected
On the date of rain, 2-3 hypophosphorous acid dihydro potassium solution (mass fraction 0.28%) is monthly sprayed, each sprayed dose is to keep plant equal
It is even to speckle with solution, after having sprayed every time per acre uniformly place 6 transmission powers be 100 watts, wave frequency 200MHz
To the electromagnetic wave transmitter of 1000MHz, the electromagnetic wave of release 2-5 hours；
2, active monascus ruber is taken, is added into culture solution, constant temperature shakes 3 days, and keeping temperature is 25 DEG C, obtains initial incubation liquid；
The water of 3 times of amounts is added in the initial incubation liquid for taking half, and potassium dihydrogen phosphate is added, and adjusting pH value is 7.5, and protease, control is added
Temperature is 50 DEG C, is digested 3 hours, centrifugal filtration obtains filtrate (1).Above-mentioned remaining initial incubation liquid is accessed into bacteria culture tank
In, culture solution and initial incubation liquid mass ratio in bacteria culture tank are 1:0.02, and above-mentioned gained filtrate (1), constant temperature 25 is added
DEG C, closed culture 15 days, obtain optimization monascus ruber strain.
3, optimization monascus ruber strain liquid is seeded in fermentation liquid, controlled at 25 DEG C, closed 15 days.
4, Chinese medicine extracting solution is added in fermentor, Chinese medicine extracting solution is by three kinds of garlic, Fructus Forsythiae, cordate houttuynia medicinal material extracts
It obtains, the mass ratio of three kinds of medicinal materials is 1:3:3, and ultrasonic vibration simultaneously stirs evenly, and controlled at 25 DEG C, pigment bacillus bacterium is added
Kind, closed 15 days.
5, by the tripod pendulum type batch centrifugal centrifugal filtration of above-mentioned fermentation liquid, revolving speed 5000-8000r/min, sieve less than 200 mesh,
Weigh a certain amount of salicylic acid, magnesium sulfate and cumarin again after filtrate and be dissolved into filtrate, make water in dissolved aqueous solution
The concentration of poplar acid is 35ppm, and the mass fraction of magnesium sulfate is 0.04%, and the mass fraction of cumarin is 0.07%；The 3-10 phase in month
Between, the medicament of above-mentioned preparation is selected into the date without rain, carries out foliage-spray toward the bletilla striata stem tuber being growing, before sunrise or
Post sunset is sprayed, and is monthly sprayed 1-2 times, sprays interval every time not less than 10 days, each sprayed dose is to keep plant uniform
Speckle with solution.
By this method carry out top dressing, foliage-spray and electromagnetic wave stimulation, bletilla striata Tuber yields than under equal conditions not into
The bletilla striata Tuber yields of row top dressing, foliage-spray and electromagnetic wave stimulation improve 23%, and polysaccharide and alkaloid in bletilla striata stem tuber etc. have
Effective component content is higher by 42% than the bletilla striata stem tuber under equal conditions without the stimulation of top dressing, foliage-spray and electromagnetic wave, pest and disease damage
Incidence reduces by 57%.
Embodiment 2
A method of bletilla striata Tuber yields and quality are improved, including the following steps:
1,4-6 month is bletilla striata stem tuber nutrient growth animated period, and the additional instant nitrogenousfertilizer in ground is primary；During 4-8 month, nothing is selected
On the date of rain, 2-3 hypophosphorous acid dihydro potassium solution (mass fraction 0.28%) is monthly sprayed, each sprayed dose is to keep plant equal
It is even to speckle with solution, after having sprayed every time per acre uniformly place 6 transmission powers be 100 watts, wave frequency 200MHz
To the electromagnetic wave transmitter of 1000MHz, the electromagnetic wave of release 2-5 hours.
2, active monascus ruber is taken, is added into culture solution, constant temperature shakes 2 days, and keeping temperature is 20 DEG C, obtains initial incubation
Liquid；The water of 2 times of amounts is added in the initial incubation liquid for taking half, and potassium dihydrogen phosphate is added, and adjusting pH value is 7, and protease, control is added
Temperature processed is 40 DEG C, is digested 2 hours, centrifugal filtration obtains filtrate (1).Above-mentioned remaining initial incubation liquid is accessed into Spawn incubation
In tank, culture solution and initial incubation liquid mass ratio in bacteria culture tank are 1:0.01, and above-mentioned gained filtrate (1), constant temperature is added
20 DEG C, closed culture 10 days, obtain optimization monascus ruber strain.
3, optimization monascus ruber strain liquid is seeded in fermentation liquid, controlled at 20 DEG C, closed 10 days.
4, Chinese medicine extracting solution is added in fermentor, Chinese medicine extracting solution is by three kinds of garlic, Fructus Forsythiae, cordate houttuynia medicinal material extracts
It obtains, the mass ratio of three kinds of medicinal materials is 1:1:1, and ultrasonic vibration simultaneously stirs evenly, and controlled at 20 DEG C, pigment bacillus bacterium is added
Kind, closed 10 days.
5, by the tripod pendulum type batch centrifugal centrifugal filtration of above-mentioned fermentation liquid, revolving speed 5000-8000r/min, sieve less than 200 mesh,
Weigh a certain amount of salicylic acid, magnesium sulfate and cumarin again after filtrate and be dissolved into filtrate, make water in dissolved aqueous solution
The concentration of poplar acid is 55ppm, and the mass fraction of magnesium sulfate is 0.05%, and the mass fraction of cumarin is 0.04%；The 3-10 phase in month
Between, the medicament of above-mentioned preparation is selected into the date without rain, carries out foliage-spray toward the bletilla striata stem tuber being growing, before sunrise or
Post sunset is sprayed, and is monthly sprayed 1-2 times, sprays interval every time not less than 10 days, each sprayed dose is to keep plant uniform
Speckle with solution.
By this method carry out top dressing, foliage-spray and electromagnetic wave stimulation, bletilla striata Tuber yields than under equal conditions not into
The bletilla striata Tuber yields of row top dressing, foliage-spray and electromagnetic wave stimulation improve 29%, and polysaccharide and alkaloid in bletilla striata stem tuber etc. have
Effective component content is higher by 55% than the bletilla striata stem tuber under equal conditions without the stimulation of top dressing, foliage-spray and electromagnetic wave, pest and disease damage
Incidence reduces by 73%.
Embodiment 3
A method of bletilla striata Tuber yields and quality are improved, including the following steps:
1,4-6 month is bletilla striata stem tuber nutrient growth animated period, and the additional instant nitrogenousfertilizer in ground is primary；During 4-8 month, nothing is selected
On the date of rain, 2-3 hypophosphorous acid dihydro potassium solution (mass fraction 0.28%) is monthly sprayed, each sprayed dose is to keep plant equal
It is even to speckle with solution, after having sprayed every time per acre uniformly place 6 transmission powers be 100 watts, wave frequency 200MHz
To the electromagnetic wave transmitter of 1000MHz, the electromagnetic wave of release 2-5 hours；
2, active monascus ruber is taken, is added into culture solution, constant temperature shakes 4 days, and keeping temperature is 30 DEG C, obtains initial incubation liquid；
The water of 4 times of amounts is added in the initial incubation liquid for taking half, and potassium dihydrogen phosphate is added, and adjusting pH value is 8, and protease, control temperature is added
Degree is 60 DEG C, is digested 4 hours, centrifugal filtration obtains filtrate (1).Above-mentioned remaining initial incubation liquid is accessed in bacteria culture tank,
Culture solution in bacteria culture tank and initial incubation liquid mass ratio are 1:0.03, are added above-mentioned gained filtrate (1), 30 DEG C of constant temperature,
Closed culture 20 days, obtain optimization monascus ruber strain.
3, optimization monascus ruber strain liquid is seeded in fermentation liquid, controlled at 30 DEG C, closed 20 days.
4, Chinese medicine extracting solution is added in fermentor, Chinese medicine extracting solution is by three kinds of garlic, Fructus Forsythiae, cordate houttuynia medicinal material extracts
It obtains, the mass ratio of three kinds of medicinal materials is 1:5:5, and ultrasonic vibration simultaneously stirs evenly, and controlled at 0 DEG C, pigment bacillus bacterium is added
Kind, closed 20 days.
5, by the tripod pendulum type batch centrifugal centrifugal filtration of above-mentioned fermentation liquid, revolving speed 5000-8000r/min, sieve less than 200 mesh,
Weigh a certain amount of salicylic acid, magnesium sulfate and cumarin again after filtrate and be dissolved into filtrate, make water in dissolved aqueous solution
The concentration of poplar acid is 70ppm, and the mass fraction of magnesium sulfate is 0.02%, and the mass fraction of cumarin is 0.06%；The 3-10 phase in month
Between, the medicament of above-mentioned preparation is selected into the date without rain, carries out foliage-spray toward the bletilla striata stem tuber being growing, before sunrise or
Post sunset is sprayed, and is monthly sprayed 1-2 times, sprays interval every time not less than 10 days, each sprayed dose is to keep plant uniform
Speckle with solution.
By this method carry out top dressing, foliage-spray and electromagnetic wave stimulation, bletilla striata Tuber yields than under equal conditions not into
The bletilla striata Tuber yields of row top dressing, foliage-spray and electromagnetic wave stimulation improve 33%, and polysaccharide and alkaloid in bletilla striata stem tuber etc. have
Effective component content is higher by 78% than the bletilla striata stem tuber under equal conditions without the stimulation of top dressing, foliage-spray and electromagnetic wave, pest and disease damage
Incidence reduces by 61%.
Above-described embodiment is not intended to limit the present invention in any form, anyone skilled in the art,
In the case where not departing from technical solution of the present invention range, all technical solution of the present invention is done using the technology contents of the disclosure above
Many possible changes and modifications or equivalent example modified to equivalent change out.Therefore, all without departing from the technology of the present invention
The content of scheme, technical spirit any simple modifications, equivalents, and modifications made to the above embodiment according to the present invention,
It should fall in the range of technical solution of the present invention protection.
Claims (7)
1. a kind of method for improving bletilla striata Tuber yields and quality, it is characterised in that including the following steps:
(1) 4-6 month is bletilla striata nutrient growth animated period, and the additional instant nitrogenousfertilizer in ground is primary；During 4-8 month, select without rain
Date, monthly spray 2-3 hypophosphorous acid dihydro potassium solution (mass fraction 0.28%), each sprayed dose is to keep plant uniform
Speckle with solution, after having sprayed every time per acre uniformly place 6 transmission powers be 100 watts, wave frequency be 200MHz extremely
The electromagnetic wave transmitter of 1000MHz, the electromagnetic wave of release 2-5 hours；
(2) optimization monascus ruber strain liquid is seeded in fermentation liquid, controlled at 20-30 DEG C, closed 9-21 days；Fermentor
Middle addition Chinese medicine extracting solution, ultrasonic vibration simultaneously stirs evenly, and controlled at 19-31 DEG C, pigment bacillus species are added, closed
9-21 days；By the tripod pendulum type batch centrifugal centrifugal filtration of above-mentioned fermentation liquid, revolving speed 5000-8000r/min, sieve must be filtered less than 200 mesh
A certain amount of salicylic acid, magnesium sulfate and cumarin are weighed after liquid again to be dissolved into filtrate, makes salicylic acid in dissolved aqueous solution
Concentration be 30-100ppm, the mass fraction of magnesium sulfate is 0.01%-0.06%, and the mass fraction of cumarin is 0.03%-
0.08%；During 3-10 month, the medicament of above-mentioned preparation is selected into the date without rain, carries out blade face spray toward the bletilla striata being growing
It applies, before sunrise or post sunset is sprayed, and is monthly sprayed 1-2 times, and interval is sprayed every time to be not less than 10 days, each sprayed dose
To make plant uniformly speckle with solution.
2. improving the method for bletilla striata Tuber yields and quality according to claim 1, it is characterised in that: the optimization monascus
Bacterium strain liquid inoculum concentration is the 2%-6% of quality of fermentation broth.
3. improving the method for bletilla striata Tuber yields and quality according to claim 2, it is characterised in that: the optimization monascus
Bacterium process for preparing strain thereof is as follows:
(1) active monascus ruber is taken, is added into culture solution, constant temperature shakes 2-4 days, and keeping temperature is 20-30 DEG C, obtains and initially trains
Nutrient solution；
(2) the initial incubation liquid of half is taken, the 2-4 times of water measured is added, potassium dihydrogen phosphate is added, adjusting pH value is 7-8, and egg is added
White enzyme digests 2-4 hours, centrifugal filtration obtains filtrate controlled at 40-60 DEG C；
(3) above-mentioned remaining initial incubation liquid is accessed in bacteria culture tank, culture solution and initial incubation in bacteria culture tank
Liquid mass ratio is 1:0.01-0.03, is added filtrate obtained by above-mentioned second step, 20-30 DEG C of constant temperature, closed culture 10-20 days, i.e.,
It can.
4. improving the method for bletilla striata Tuber yields and quality according to claim 3, it is characterised in that: the culture solution is by egg
White peptone, potassium nitrate, beef broth, dithyl sulfate, iron sulfite and water composition, composition mass ratio is successively are as follows: 1:0.1:5:
0.05:100。
5. improving the method for bletilla striata Tuber yields and quality according to claim 4, it is characterised in that: the culture solution preparation
Method is as follows: peptone, potassium nitrate, beef broth, dithyl sulfate, iron sulfite being sequentially added in water, are stirred, is heated
To 80 DEG C, 2-4 hours are kept the temperature, is bottled after cooling, at 121 DEG C, put out a fire 30min.
6. improving the method for bletilla striata Tuber yields and quality according to claim 5, it is characterised in that: the Chinese medicine extracts
Liquid is obtained by three kinds of garlic, Fructus Forsythiae, cordate houttuynia medicinal material extracts, and the mass ratio of three kinds of medicinal materials is 1:1-5:1-5.
7. improving the method for bletilla striata Tuber yields and quality according to claim 6, it is characterised in that: the Chinese medicine extracts
Liquid and preparation method thereof is as follows: taking appropriate garlic, Fructus Forsythiae, cordate houttuynia, is added and is equivalent to garlic, Fructus Forsythiae, 2-4 times of cordate houttuynia gross mass
The ethanol solution of amount, heating and refluxing extraction 1-3 times is 2-4 hours each, and concentration of alcohol 60%-80%, merging filtrate is concentrated into
Without ethanol flavor.
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| CN101210046A (en) * | 2006-12-27 | 2008-07-02 | 财团法人食品工业发展研究所 | Antifungal protein and usage thereof |
| CN106982633A (en) * | 2017-04-17 | 2017-07-28 | 胡江宇 | The method that Tripterospermum cordatum herb yield and quality are improved using Ultrasonic Radiation |
| CN107135830A (en) * | 2017-05-10 | 2017-09-08 | 胡江宇 | The method that Honeysuckle flower yield and quality are improved using electromagenetic wave radiation |
| CN107285831A (en) * | 2017-06-23 | 2017-10-24 | 宁国市仙之居家庭农场 | The fertilizing method of bletilla striata plantation |
| CN107278595A (en) * | 2017-08-01 | 2017-10-24 | 广西桂平市石龙镇江夏种养专业合作社 | A kind of standardized cultivation method of bletilla striata high yield |
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