CN103304298B - Hericium erinaceus mother strain culture medium and making method thereof - Google Patents
Hericium erinaceus mother strain culture medium and making method thereof Download PDFInfo
- Publication number
- CN103304298B CN103304298B CN201310237936.1A CN201310237936A CN103304298B CN 103304298 B CN103304298 B CN 103304298B CN 201310237936 A CN201310237936 A CN 201310237936A CN 103304298 B CN103304298 B CN 103304298B
- Authority
- CN
- China
- Prior art keywords
- hericium erinaceus
- mother
- pers
- bull
- culture medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a hericium erinaceus mother strain culture medium. The hericium erinaceus mother strain culture medium is characterized in that melissyl alcohol with certain concentration is added to a PDA (Potato Dextrose Agar) enriched medium. The invention also relates to a making method of the hericium erinaceus mother strain culture medium. Compared with an existing PDA culture medium, the hericium erinaceus mother strain culture medium disclosed by the invention has the advantages that the growth vitality of hericium erinaceus mycelium is enhanced because the melissyl alcohol stimulates the energy metabolism of mycelium, so that the fungi culture time of a mother strain is shortened, the fungi culture time of an original strain is also shortened by making a hericium erinaceus original strain by using the mother strain, therefore, the differentiation phenomenon of a sporocarp during a strain germination period is avoided, the nutrient loss is reduced and the strain quality is improved.
Description
Technical field
Content of the present invention comprises Hericium erinaceus (Bull. Ex Fr.) Pers. Mother culture based formulas, and the making method of this substratum, belongs to edible mashroom cultivating technical field.
Background technology
Hericium erinaceus (Bull. Ex Fr.) Pers., sporophore is round and thick, is covered with needle-like bacterium thorn, the pole of figure like the head of monkey, so gain the name.Hericium erinaceus (Bull. Ex Fr.) Pers. has the title of mountain delicacy, is a kind of food of top grade, the dual-purpose bacterium of medicine.It can cure the diseases such as maldigestion, stomach ulcer and neurasthenia.Recent domestic medical evidence, the malignant tumour of Hericium erinaceus (Bull. Ex Fr.) Pers. to the Digestive tract such as cancer of the stomach, esophagus cancer has good auxiliary curative effect, has monkey mushroom sheet etc. with the good medicine that it is made.
Growing of Hericium erinaceus (Bull. Ex Fr.) Pers., relatively stricter to the requirement of nutritional condition, under different nutritional conditions, the power of mycelial growth, dense, speed difference is very large.Especially the technical difficulty that in Hericium erinaceus (Bull. Ex Fr.) Pers. scale operation, bacterial classification makes is larger, because the quality of bacterial classification is unstable, causes Hericium erinaceus (Bull. Ex Fr.) Pers. output also very unstable.
Make Hericium erinaceus (Bull. Ex Fr.) Pers. mother with the PDA substratum of routine to plant, mycelia often forms irregular pallial siphuncle, the speed of growth is very slow, mycelia quantity is little, need through the cultivation of more than 20d, mycelia just can cover with test tube slant, and now, inclined-plane has defined a lot of goose pimples-fruit body primordium, and namely there is catabiosis in bacterial classification.After planting access hedgehog hydnum original seed with this mother, mycelial growth is sparse, very thin, skewness, and mycelia not yet sends out half full often, and culture material just starts to form former base, causes nutrient to consume in a large number, and spawn activity declines.
Triacontanol price quote is a kind of natural, nontoxic, efficient plant-growth regulator, uses trace level and just can produce appreciable results, can improve multiple enzymic activity, promotes C N metabolism effect.
The present invention adopts PDA enriched medium, adds certain density triacontanol price quote, stimulates mycelium energy metabolism, accelerates the absorption of nutritive substance, conversion, and Hericium erinaceus (Bull. Ex Fr.) Pers. filament length speed is accelerated and dense pure white greatly, no longer forms shoestring and former base.After this bacterial classification access hedgehog hydnum original seed, mycelial growth is vigorous, healthy and strong, and the full bottle time shorten of bacterial classification, improves strain quality.
Summary of the invention
The present invention has following two aspects:
1, a kind of Hericium erinaceus (Bull. Ex Fr.) Pers. mother culture media
2, the making method of substratum
The technical solution adopted in the present invention is:
A kind of Hericium erinaceus (Bull. Ex Fr.) Pers. Mother culture based formulas: PDA enriched medium (potato 200g, glucose 20g, potassium primary phosphate 3g, agar 20g, tap water 1000ml), then add substratum total mass 0.2mg/kg triacontanol price quote.
Triacontanol price quote: be content 0.1% microemulsion, 0.1% triacontanol price quote stoste sterilized water is diluted 100 times, obtains 10mg/kg and dilutes mother liquor, then use membrane filter appts filtration sterilization.
Potassium primary phosphate: be chemically pure reagent.
The making method of substratum, comprises the steps:
1. potato wash clean, peeling, digs up eye, balance takes 200g, be cut into the fritter of 1cm-2cm;
2. the potato cut is poured in aluminum pot, add water 1000ml, and reburn after boiling 15min-20min, not rotten for degree with potato shortcake;
3. use 4 layers of filtered through gauze, get filtrate, put into 20g agar, be heated to agar and dissolve completely, then use 4 layers of filtered through gauze;
4. filtrate adds water to 1000mL, adds glucose 20g, potassium primary phosphate 3g, and heating is dissolved, and stirs;
5. be sub-packed in while hot in the test tube of Φ 20mm × 200mm, often propping up test tube dispensed loading amount is 5ml, then cotton plug beyond the Great Wall;
6. autoclaving, pressure rises to 1.05kg/cm
2, temperature reaches 121 DEG C-126 DEG C, maintains 20min-30min, after taking the dish out of the pot, puts into Bechtop, slightly cool;
7. treat that substratum is cooled to about 70 DEG C, often prop up test tube and add the dilution of the triacontanol price quote after filtration sterilization mother liquor 100 μ l, the liquid-transfering gun crossed by sterilising treatment adds, and then shakes up (substratum not being splashed cotton plug), puts inclined-plane while hot;
8., after inoculation, culture temperature 20 DEG C-22 DEG C, cultivate through 7d-8d, hericium mycelium covers with inclined-plane.
Plant access Primary spawn material with this hedgehog hydnum mother, owing to containing plant hormone triacontanol price quote in Mother culture base-material, have stimulated mycelial growth, mycelial growth is vigorous, the full bottle time shorten of original seed.
A kind of Hericium erinaceus (Bull. Ex Fr.) Pers. mother provided by the invention plants and makes novel method, in PDA enriched medium, add triacontanol price quote, due to the effect of plant hormone, enhance the growth vigor of hericium mycelium, make female kind cultivation time shorten, plant with this mother and make Hericium erinaceus (Bull. Ex Fr.) Pers. original seed, the cultivation time of original seed shortens too, avoids bacteria developing period fruiting body differentiation phenomenon, decrease the loss of nutrient, improve the quality of bacterial classification.
Embodiment
A kind of Hericium erinaceus (Bull. Ex Fr.) Pers. Mother culture based formulas in the present embodiment: potato 200g, glucose 20g, potassium primary phosphate 3g, agar 20g, tap water 1000ml, then add substratum (finished product) total mass 0.2mg/kg triacontanol price quote.
Making method:
1. potato wash clean, peeling, digs up eye, balance takes 200g, be cut into the fritter of 1cm-2cm;
2. the potato cut is poured in aluminum pot, add water 1000ml, and reburn after boiling 20min, not rotten for degree with potato shortcake;
3. use 4 layers of filtered through gauze, get filtrate, put into 20g agar, be heated to agar and dissolve completely, then use 4 layers of filtered through gauze;
4. filtrate adds water to 1000mL, adds glucose 20g, potassium primary phosphate 3g, and heating is dissolved, and stirs;
5. be sub-packed in while hot in the test tube of Φ 20mm × 200mm, often propping up test tube dispensed loading amount is 5ml, then cotton plug beyond the Great Wall;
6. autoclaving, pressure rises to 1.05kg/cm
2, temperature reaches 125 DEG C, maintains 25min, after taking the dish out of the pot, puts into Bechtop, slightly cool;
7. in Bechtop, 0.1% triacontanol price quote stoste sterilized water is diluted 100 times, obtain 10mg/kg and dilute mother liquor, then use membrane filter appts filtration sterilization;
8. treat that substratum is cooled to about 70 DEG C, often prop up test tube and add the dilution of the triacontanol price quote after filtration sterilization mother liquor 100 μ l, the liquid-transfering gun crossed by sterilising treatment adds, and then shakes up (substratum not being splashed cotton plug), puts inclined-plane while hot;
9., after inoculation, culture temperature 22 DEG C, cultivate through 7d, hericium mycelium covers with inclined-plane.
Plant access Primary spawn material with this hedgehog hydnum mother, owing to containing plant hormone triacontanol price quote in Mother culture base-material, have stimulated mycelial growth, mycelial growth is vigorous, and through a bacterium of 15d, mycelia sends out completely full bottle.
Claims (4)
1. a Hericium erinaceus (Bull. Ex Fr.) Pers. mother culture media, is characterized in that containing, for example lower component and quality proportioning: potato 200g, glucose 20g, potassium primary phosphate 3g, agar 20g, tap water 1000ml, then adds substratum total mass 0.2mg/kg triacontanol price quote.
2. Hericium erinaceus (Bull. Ex Fr.) Pers. mother culture media according to claim 1, it is characterized in that described triacontanol price quote prepares gained by the following method: 0.1% triacontanol price quote stoste sterilized water is diluted 100 times, obtain 10mg/kg and dilute mother liquor, then use membrane filter appts filtration sterilization.
3. Hericium erinaceus (Bull. Ex Fr.) Pers. mother culture media according to claim 1, is characterized in that described potassium primary phosphate is chemically pure reagent.
4. a making method for the Hericium erinaceus (Bull. Ex Fr.) Pers. mother culture media according to any one of claim 1-3, is characterized in that:
1. potato wash clean, peeling, digs up eye, balance takes 200g, be cut into the fritter of 1cm-2cm;
2. the potato cut is poured in aluminum pot, add water 1000ml, and reburn after boiling 15min-20min, not rotten for degree with potato shortcake;
3. use 4 layers of filtered through gauze, get filtrate, put into 20g agar, be heated to agar and dissolve completely, then use 4 layers of filtered through gauze;
4. filtrate adds water to 1000mL, adds glucose 20g, potassium primary phosphate 3g, and heating is dissolved, and stirs;
5. be sub-packed in while hot in the test tube of Φ 20mm × 200mm, often propping up test tube dispensed loading amount is 5ml, then cotton plug beyond the Great Wall;
6. autoclaving, pressure rises to 1.05kg/cm
2, temperature reaches 121 DEG C-126 DEG C, maintains 20min-30min, after taking the dish out of the pot, puts into Bechtop, slightly cool;
7. treat that substratum is cooled to about 70 DEG C, often prop up test tube and add the dilution of the triacontanol price quote after filtration sterilization mother liquor 100 μ l, the liquid-transfering gun crossed by sterilising treatment adds, and then shakes up, puts inclined-plane while hot;
8., after inoculation, culture temperature 20 DEG C-22 DEG C, cultivate through 7d-8d, hericium mycelium covers with inclined-plane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310237936.1A CN103304298B (en) | 2013-06-02 | 2013-06-02 | Hericium erinaceus mother strain culture medium and making method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310237936.1A CN103304298B (en) | 2013-06-02 | 2013-06-02 | Hericium erinaceus mother strain culture medium and making method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103304298A CN103304298A (en) | 2013-09-18 |
| CN103304298B true CN103304298B (en) | 2014-12-24 |
Family
ID=49130060
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310237936.1A Expired - Fee Related CN103304298B (en) | 2013-06-02 | 2013-06-02 | Hericium erinaceus mother strain culture medium and making method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103304298B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111084054A (en) * | 2020-02-21 | 2020-05-01 | 铜仁学院 | Method for preparing hericium erinaceus strain from Huangjiang flower fungus material |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104823710A (en) * | 2015-04-18 | 2015-08-12 | 余臣义 | Preparation method of hericium erinaceus mycelium powder |
| CN105130594A (en) * | 2015-08-10 | 2015-12-09 | 呼伦贝尔生态产业技术研究院 | Preparation method of high quality medium for edible-medicinal fungus first generation production mother culture and medium |
| CN105693330A (en) * | 2016-02-04 | 2016-06-22 | 湖南省宇秀生物科技有限公司 | High-transparency PDA (potato dextrose agar) culture medium preparing method |
| CN107691110A (en) * | 2017-11-28 | 2018-02-16 | 南宁市生润科技有限公司 | The breeding method of monkey mushroom bacterium |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03178901A (en) * | 1989-12-06 | 1991-08-02 | Teijin Ltd | Method for improving taste of rice grain |
| CN101683046A (en) * | 2008-09-22 | 2010-03-31 | 赵伟 | Method for preparing female seed of hericium erinaceum |
| CN101971762B (en) * | 2010-02-09 | 2012-01-11 | 中国农业科学院农业资源与农业区划研究所 | Liquid submerged fermentation culture method for hericium erinaceus |
| CN101941868A (en) * | 2010-08-31 | 2011-01-12 | 姚平生 | Edible fungus culture medium |
-
2013
- 2013-06-02 CN CN201310237936.1A patent/CN103304298B/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111084054A (en) * | 2020-02-21 | 2020-05-01 | 铜仁学院 | Method for preparing hericium erinaceus strain from Huangjiang flower fungus material |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103304298A (en) | 2013-09-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102010240B (en) | Plant enzyme foliar fertilizer and preparation method thereof | |
| CN103304298B (en) | Hericium erinaceus mother strain culture medium and making method thereof | |
| CN104350954B (en) | Cornu Cervi Pantotrichum mushroom cultivation method | |
| CN105230430B (en) | A kind of Se accumulation efficient cultivation method of blue or green money willow | |
| CN104987156B (en) | A kind of method of binwang mushroom culture medium and cultivation binwang mushroom using mushroom bran | |
| CN106576871A (en) | Planting method for selenium-rich cucumber | |
| CN105110961A (en) | Liquid fermentation culture medium for shiitake mushrooms and method for producing shiitake mushrooms through the same | |
| CN109337895A (en) | A kind of production method of good quality and high output selenium-enriched hericium erinaceus strain | |
| CN103250554B (en) | Method for cultivating walnut shell powder bag material healthcare needle mushrooms | |
| CN103250556A (en) | Method for cultivating rhodiola rosea bag material healthcare pleurotus nebrodensis | |
| CN104255299A (en) | Method for cultivating cordyceps militaris | |
| CN102498833A (en) | High-yield cultivation method for sweet potato in greenhouse | |
| CN107172909A (en) | A kind of extensive breeding method of pumpkin rice shoot | |
| CN103283486B (en) | Pleurotus eryngii ridge-up bed raw material soil-covering cultivation technology | |
| CN107827529A (en) | A kind of fertilizing method for improving black rice content of physiologically active substance | |
| CN107439300A (en) | A kind of method of Loquat Seeds nursery | |
| CN103250562B (en) | Method for cultivating boxthorn root bag material healthcare mushrooms | |
| CN110150120A (en) | Alpha-aminobutyric acid content and the production method of yield is improved in a kind of enrichment beans bean sprout | |
| CN109105148A (en) | A kind of high yield and high quality cordyceps militaris plantation culture medium and its cultural method | |
| CN109006182A (en) | A kind of culture base-material and preparation method thereof with Lenlinus edodes slag for cultivating oyster mushroom | |
| CN107047067A (en) | A kind of cultural method of edible mushroom | |
| CN106588290A (en) | Culture medium for flammulina velutipes | |
| CN102630480A (en) | Iron-rich black fungus and production method thereof | |
| CN108849227A (en) | A kind of cultural method of dictyophora phalloidea | |
| CN109105180A (en) | A kind of implantation methods of high-yield high-quality crowndaisy chrysanthemum |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141224 Termination date: 20150602 |
|
| EXPY | Termination of patent right or utility model |