CN110150120A - Alpha-aminobutyric acid content and the production method of yield is improved in a kind of enrichment beans bean sprout - Google Patents
Alpha-aminobutyric acid content and the production method of yield is improved in a kind of enrichment beans bean sprout Download PDFInfo
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- CN110150120A CN110150120A CN201910469648.6A CN201910469648A CN110150120A CN 110150120 A CN110150120 A CN 110150120A CN 201910469648 A CN201910469648 A CN 201910469648A CN 110150120 A CN110150120 A CN 110150120A
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- sodium chloride
- germination
- production method
- aqueous solution
- aminobutyric acid
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Biodiversity & Conservation Biology (AREA)
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Abstract
The invention discloses alpha-aminobutyric acid content in a kind of enrichment beans bean sprout and improve the production method of yield, including using the beans seed for having germinating capacity as raw material, after sterilized immersion, the tall scrawny person rich in γ-aminobutyric acid is made through the joint sodium chloride processing germination of exogenous biological amine again under the conditions of temperature control is protected from light.The features such as present invention has simple process, and production cost is low, and alpha-aminobutyric acid content is high and tall scrawny person's yield is high, is suitable for health-food-level γ-aminobutyric acid tall scrawny person and produces.
Description
Technical field
The invention belongs to beans bean sprout production method technical fields, and in particular to gamma-amino in a kind of enrichment beans bean sprout
Butyric acid content and the production method for improving yield.
Background technique
Bean sprouts vegetables can not only carry out the workshop-based production of traditional-family, but also can carry out modernization industry formula production.Usual bean sprout
Growth course relies primarily on the nutrient that seed is stored, and need not generally apply fertilizer on cultivation management, and production operation is easy, only need to be suitable
Under suitable temperature environment, guarantee its moisture supply, therefore typically now use Soilless Culture Methods, in certain facility condition or interior
It carries out.
γ-aminobutyric acid (GABA) is a kind of nonprotein amino acid, other than numerous healthcare functions, to some diseases
There is apparent therapeutic effect.GABA is widely present in the animals and plants of nature, but its content is extremely low.Although the mankind are from nature
The food of middle acquisition nearly all contains GABA, but GABA is only absorbed from natural food, and content is not able to satisfy human body requirements.It grinds
The growth for studying carefully the increase and age for showing human body stress can all seriously affect the accumulation of GABA in human body, in modern fast
People need the supplement of daily carry out GABA for the demand of health in rhythm life.
Currently, the domestic report in relation to improving GABA content in plant, for example, patent (1072118 52A of publication number CN,
Publication date on 09 29th, 2017) " a kind of production method for being enriched with γ-aminobutyric acid in fructus hordei germinatus " is disclosed, patent is (open
Number CN106922509A, discloses on 07 07th, 2017) it discloses and " alpha-aminobutyric acid content and is mentioned in a kind of enrichment tall scrawny person
The production method of high yield ", patent (publication number CN 103393013B, publication date on 08 13rd, 2014) disclose a kind of " richness
The preparation method and products thereof of the germination wheat containing γ-aminobutyric acid ", and patent (publication number CN102919376A, publication date 2013
February 13) disclose " a kind of high gamma-aminobutyric acid plumule soya-bean milk and its production technology " etc. be all by plants enriched method come
Promote GABA content.GABA content in plant is improved using abiotic stress such as hypoxemia, low temperature and salt stress has become raising
The research hotspot of GABA content, but while GABA content improves, bean sprouts vegetables yield is lower, the need being unable to meet production
It asks.
Summary of the invention
Goal of the invention: in view of the above technical problems, the present invention provides γ-aminobutyric acids in a kind of enrichment beans bean sprout to contain
The production method of yield is measured and improved, is improved although being able to solve GABA content in the vegetables of bean sprouts, stress and bring beans
The technical issues of sprouts of vegetable low output.
Technical solution: in order to achieve the above object of the invention, the technical solution adopted in the present invention is as follows:
Alpha-aminobutyric acid content and the production method of yield is improved in a kind of enrichment beans bean sprout, including to have germination
The beans seed of ability is raw material, after sterilized immersion, is combined at sodium chloride through exogenous biological amine again under the conditions of temperature control is protected from light
Haircut bud, is made the tall scrawny person rich in γ-aminobutyric acid.
As preferred:
The beans seed for having germinating capacity selects the beans seed that full grains, germination percentage are high, rot without disease pest
Grain.
The method that the disinfection is impregnated are as follows: sterilized through aqueous sodium hypochlorite solution, be subsequently placed in pure water and carry out conventional leaching
Bubble processing.
Method under the conditions of temperature control is protected from light again through the joint sodium chloride processing germination of exogenous biological amine, including following step
It is rapid:
Be protected from light germination 2~6 days, be handled as follows during germination: germination the 1st day in 30~35 DEG C of conditions of germination temperature
Under, every 12~the once mixed aqueous solution containing sodium chloride and biogenic amine is sprayed for 24 hours；The 2nd~4 day is germinateed in germination temperature 30
Under the conditions of~35 DEG C, the once mixed aqueous solution containing sodium chloride and biogenic amine is sprayed every 10~18h；Germinate the 5th~6 day
Under the conditions of 25~30 DEG C of germination temperature, the once mixed aqueous solution containing sodium chloride and biogenic amine is sprayed every 3~12h.It is preferred that
It is protected from light germination 5~6 days.
The above different spraying method is standard according to the number of days for being protected from light germination, such as germination number of days is one day, then only presses
According to the 1st day method, the 2-4 days and the 5-6 days methods were not present；If germinate number of days be 2 days, the 2nd day according to
2nd~4 day method sprays, and the 5-6 days methods are not present, and so on.
Further, in terms of 30~200g of beans seed dosage, the dosage and concentration of the mixed aqueous solution sprayed are as follows:
Described 1st day, mixed 200~500ml of aqueous solution is sprayed every time, wherein containing 20~40mmol/L sodium chloride and 0.05
~0.2mmol/L biogenic amine；
Described 2nd~4 day, spray mixed 500~1000mL of aqueous solution every time, wherein containing 30~50mmol/L sodium chloride and
0.2~0.4mmol/L biogenic amine；
Described 5th~6 day, mixed 1000~1500mL of aqueous solution is sprayed every time, wherein 40~60mmol/L sodium chloride and 0.4
~0.6mmol/L biogenic amine.
The biogenic amine is selected from the one or more of spermidine, spermine and putrescine.
Tall scrawny person made from the production method, a length of 25~200mm of single plant bud, single-strain fresh weight are 0.4~2g, gamma-amino
Butyric acid content is 0.8~2mg/g DW
Technical effect: compared with the existing technology, present invention has the advantage that
1) tall scrawny person produced by the invention is rich in γ-aminobutyric acid, significantly improves the nutritive value of product, has good
Healthcare function.
2) present invention regulates and controls sodium chloride processing by exogenous biological amine during beans Grain germination, can be realized tall scrawny person
The yield of tall scrawny person is improved while middle γ-aminobutyric acid efficiently concentrating.
Detailed description of the invention
The content of morphological feature figure (A) and SOD vigor and GABA when Fig. 1 is tall scrawny person 4d and 6d under different disposal
Scheme (B), wherein Na is indicated on the basis of present invention method, is removed the biogenic amine in mixed aqueous solution, is only used sodium chloride
Processing；Spd+Na expression is handled according to present invention method；CK is indicated on the basis of present invention method, removes chlorine
Change sodium and biogenic amine, Pure water preparation is only used, as control；A, b, c indicate significant inspection result, and different lowercases indicate identical
Difference reaches the level of signifiance (p < 0.05) between different disposal under germinating time.
Specific embodiment
Technical solutions according to the invention are further described in detail below by specific embodiment.
Embodiment 1:
Full grains are selected, the mung bean seed of no disease and pests harm sterilizes it with aqueous sodium hypochlorite solution by well known method,
It is subsequently placed in the pure water of 3~5 times of amounts (v/w), soaked 50g mung bean seed is placed in sprouting machine, germination 3 is protected from light
It, is handled as follows during germination, once contains 20mmol/L under the conditions of 30 DEG C of germination temperature, every spraying for 24 hours within the 1st day
The mixed aqueous solution 200ml of sodium chloride and 0.08mmol/L spermidine, the 2nd~3 day under the conditions of 32 DEG C of germination temperature, every
12h sprays the once mixed aqueous solution 800ml containing 35mmol/L sodium chloride and 0.3mmol/L spermidine, be made growing way it is excellent,
The neat tall scrawny person of sprout, a length of 50mm of single plant bud, single-strain fresh weight 0.4g, alpha-aminobutyric acid content is 0.9mg/g DW.
Embodiment 2:
Full grains are selected, the soya bean seed of no disease and pests harm sterilizes it with aqueous sodium hypochlorite solution by well known method,
It is subsequently placed in the pure water of 3~5 times of amounts (v/w), soaked 80g soya bean seed is placed in sprouting machine, germination 4 is protected from light
It, is handled as follows during germination, under the conditions of 31 DEG C of germination temperature, sprays every 20h and once contains 22mmol/L within the 1st day
The mixed aqueous solution 300ml of sodium chloride and 0.1mmol/L spermidine, the 2nd~4 day under the conditions of 35 DEG C of germination temperature, every 15h
The once mixed aqueous solution 600ml containing 42mmol/L sodium chloride and 0.35mmol/L spermidine is sprayed, is made that growing way is excellent, bud
The neat tall scrawny person of body, a length of 60mm of single plant bud, single-strain fresh weight 0.8g, alpha-aminobutyric acid content is 1.0mg/g DW.
Embodiment 3:
Full grains are selected, the pea seed of no disease and pests harm sterilizes it with aqueous sodium hypochlorite solution by well known method,
It is subsequently placed in the pure water of 3~5 times of amounts (v/w), soaked 100g pea seed is placed in sprouting machine, germination 4 is protected from light
It, is handled as follows during germination, under the conditions of 33 DEG C of germination temperature, sprays every 13h and once contains 40mmol/L within the 1st day
The mixed aqueous solution 400ml of sodium chloride and 0.2mmol/L spermidine, the 2nd~4 day under the conditions of 33 DEG C of germination temperature, every 13h
The once mixed aqueous solution 950ml containing 38mmol/L sodium chloride and 0.38mmol/L spermidine is sprayed, is made that growing way is excellent, bud
The neat tall scrawny person of body, a length of 55mm of single plant bud, single-strain fresh weight 1.2g, alpha-aminobutyric acid content is 1.0mg/g DW.
Embodiment 4:
Full grains are selected, the soya bean seed of no disease and pests harm sterilizes it with aqueous sodium hypochlorite solution by well known method,
It is subsequently placed in the pure water of 3~5 times of amounts (v/w), soaked 150g soya bean seed is placed in sprouting machine, germination 5 is protected from light
It, is handled as follows during germination, under the conditions of 34 DEG C of germination temperature, sprays every 14h and once contains 30mmol/L within the 1st day
The mixed aqueous solution 450ml of sodium chloride and 0.15mmol/L spermidine, the 2nd~4 day under the conditions of 32 DEG C of germination temperature, every
17h sprays the once mixed aqueous solution 800ml containing 35mmol/L sodium chloride and 0.4mmol/L spermidine, and the 5th day in germination temperature
Under the conditions of 30 DEG C of degree, the once mixed aqueous solution containing 45mmol/L sodium chloride and 0.47mmol/L spermidine is sprayed every 5h
1300ml, is made growing way is excellent, sprout is neat tall scrawny person, single plant bud a length of 65mm, single-strain fresh weight 1.5g, gamma-amino
Butyric acid content is 1.5mg/g DW.
Embodiment 5:
Full grains are selected, the pea seed of no disease and pests harm sterilizes it with aqueous sodium hypochlorite solution by well known method,
It is subsequently placed in the pure water of 3~5 times of amounts (v/w), soaked 160g pea seed is placed in sprouting machine, germination 6 is protected from light
It, is handled as follows during germination, under the conditions of 31 DEG C of germination temperature, sprays every 12h and once contains 26mmol/L within the 1st day
The mixed aqueous solution 280ml of sodium chloride and 0.12mmol/L spermidine, the 2nd~4 day under the conditions of 33 DEG C of germination temperature, every
11h sprays the once mixed aqueous solution 1000ml containing 34mmol/L sodium chloride and 0.36mmol/L spermidine, the 5th~6 day
Under the conditions of 27 DEG C of germination temperature, the once mixing water containing 43mmol/L sodium chloride and 0.48mmol/L spermidine is sprayed every 8h
The tall scrawny person that growing way is excellent, sprout is neat is made in solution 1500ml.The a length of 80mm of single plant bud in tall scrawny person produced by the present invention,
Single-strain fresh weight is 1.8g, and alpha-aminobutyric acid content is 1.8mg/g DW.
Claims (7)
1. alpha-aminobutyric acid content and improving the production method of yield in a kind of enrichment beans bean sprout, which is characterized in that including with
The beans seed for having germinating capacity is raw material, after sterilized immersion, is combined again through exogenous biological amine under the conditions of temperature control is protected from light
Sodium chloride processing germination, is made the tall scrawny person rich in γ-aminobutyric acid.
2. production method according to claim 1, which is characterized in that the beans seed for having germinating capacity, selection
The beans seed that full grains, germination percentage are high, rot without disease pest.
3. production method according to claim 1, which is characterized in that the method that the disinfection is impregnated are as follows: through sodium hypochlorite
Water-soluble liquid disinfectant is subsequently placed in pure water and carries out conventional immersion treatment.
4. production method according to claim 1, which is characterized in that it is described under the conditions of temperature control is protected from light again through exogenous biological
Amine combines the method for sodium chloride processing germination, comprising the following steps:
It is protected from light germination 2~6 days, is handled as follows during germination: germinateing the 1st day under the conditions of 30~35 DEG C of germination temperature, often
Every 12~the once mixed aqueous solution containing sodium chloride and biogenic amine is sprayed for 24 hours；The 2nd~4 day is germinateed in germination temperature 30~35
Under the conditions of DEG C, the once mixed aqueous solution containing sodium chloride and biogenic amine is sprayed every 10~18h；It germinates the 5th~6 day and is germinateing
Under the conditions of 25~30 DEG C of temperature, the once mixed aqueous solution containing sodium chloride and biogenic amine is sprayed every 3~12h.
5. production method according to claim 4, which is characterized in that in terms of 30~200g of beans seed dosage, spray
The dosage and concentration of mixed aqueous solution are as follows:
Described 1st day, mixed 200~500ml of aqueous solution was sprayed every time, wherein containing 20~40mmol/L sodium chloride and 0.05~
0.2mmol/L biogenic amine；
Described 2nd~4 day, mixed 500~1000mL of aqueous solution is sprayed every time, wherein containing 30~50mmol/L sodium chloride and 0.2
~0.4mmol/L biogenic amine；
Described 5th~6 day, mixed 1000~1500mL of aqueous solution was sprayed every time, wherein 40~60mmol/L sodium chloride and 0.4~
0.6mmol/L biogenic amine.
6. production method according to claim 1, which is characterized in that the biogenic amine is selected from spermidine, spermine and putrescine
One or more.
7. production method according to claim 1, which is characterized in that tall scrawny person made from the production method, single plant bud are long
For 25~200mm, single-strain fresh weight is 0.4~2g, and alpha-aminobutyric acid content is 0.8~2mg/g DW.
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| CN201910469648.6A CN110150120A (en) | 2019-05-31 | 2019-05-31 | Alpha-aminobutyric acid content and the production method of yield is improved in a kind of enrichment beans bean sprout |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112515018A (en) * | 2019-09-18 | 2021-03-19 | 南京农业大学 | Production technology of barley tea beverage rich in phenolic acid |
| CN114846953A (en) * | 2022-05-25 | 2022-08-05 | 扬州大学 | Method for enriching functional substances in soybean sprouts and improving yield |
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| WO2015005562A1 (en) * | 2013-07-12 | 2015-01-15 | 경상대학교 산학협력단 | Therapeutic agent for neurological disorders including, as active ingredients, anthocyanin and gabab receptor agonist |
| CN106922509A (en) * | 2017-03-16 | 2017-07-07 | 扬州大学 | It is a kind of to be enriched with tall scrawny person gamma aminobutyric acid content and improve the production method of yield |
| KR20190020381A (en) * | 2017-08-21 | 2019-03-04 | 경남과학기술대학교 산학협력단 | Preparation method for soybean-fermented composition having enhanced GABA, Conjugated linoleic acid and Aglycone isoflavones, combined probiotics for its fermentation and functional food of anti-diabetic and anti-obesity effects containing the soybean-fermented composition |
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2019
- 2019-05-31 CN CN201910469648.6A patent/CN110150120A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2015005562A1 (en) * | 2013-07-12 | 2015-01-15 | 경상대학교 산학협력단 | Therapeutic agent for neurological disorders including, as active ingredients, anthocyanin and gabab receptor agonist |
| CN106922509A (en) * | 2017-03-16 | 2017-07-07 | 扬州大学 | It is a kind of to be enriched with tall scrawny person gamma aminobutyric acid content and improve the production method of yield |
| KR20190020381A (en) * | 2017-08-21 | 2019-03-04 | 경남과학기술대학교 산학협력단 | Preparation method for soybean-fermented composition having enhanced GABA, Conjugated linoleic acid and Aglycone isoflavones, combined probiotics for its fermentation and functional food of anti-diabetic and anti-obesity effects containing the soybean-fermented composition |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112515018A (en) * | 2019-09-18 | 2021-03-19 | 南京农业大学 | Production technology of barley tea beverage rich in phenolic acid |
| CN112515018B (en) * | 2019-09-18 | 2023-06-06 | 南京农业大学 | Production technology of barley tea beverage rich in phenolic acid |
| CN114846953A (en) * | 2022-05-25 | 2022-08-05 | 扬州大学 | Method for enriching functional substances in soybean sprouts and improving yield |
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Application publication date: 20190823 |