CN105130594A - Preparation method of high quality medium for edible-medicinal fungus first generation production mother culture and medium - Google Patents
Preparation method of high quality medium for edible-medicinal fungus first generation production mother culture and medium Download PDFInfo
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- CN105130594A CN105130594A CN201510489105.2A CN201510489105A CN105130594A CN 105130594 A CN105130594 A CN 105130594A CN 201510489105 A CN201510489105 A CN 201510489105A CN 105130594 A CN105130594 A CN 105130594A
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Abstract
The invention discloses a preparation method of a high quality medium for an edible-medicinal fungus first generation production mother culture and a medium. The preparation method of the high quality medium for the edible-medicinal fungus first generation production mother culture comprises the steps of: (1) preparation of sawdust liquid; (2) component weighing and preparation; and (3) subpackaging and sterilization. The medium provided by the invention can promote the growth of solid tissue and mycelia and promote the spore germination rate, and has no residual toxicity and growth promoting effect. The medium can remove viruses, prevent sundry fungus growth, does not produce latent viruses and sundry fungi, and can accelerate the hypha feeding speed. The cultured mycelia are pure white, thick and strong, grow densely, have neat edges, and short full pipe time. The primordium differentiation is rapid, the growth of fruiting bodies can be promoted, and then the yield is increased.
Description
Technical field:
The present invention relates to a kind of substratum compound method and substratum, be specifically related to high-quality substratum compound method and substratum that an a kind of edible medicinal fungus generation produces female kind.
Background technology:
Along with edible medicinal fungus science and technology leap development, people and scientific worker's understanding in depth and opening up edible medicinal fungus, the demand of some rare mushrooms is constantly increased, wild mushroom class is fewer and feweri, to the mushroom ear class of those preciousnesses, science and technology domestication must be adopted, carry out artificial creation's condition, meet the ecological condition of mushroom ear class growth and development, implement artificial culture and cultivation.Technology is updated, constantly bring forth new ideas, scientists adopts advanced biofermentation technique to be used for edible medicinal fungus field, liquid submerged fermentation is carried out to rare mushroom ear class, make liquid spawn, and its effective constituent is extracted, be processed into by rare mushroom effective constituent the needs that healthcare products and nutritious prod meet people, increase good fortune location and the economic benefit of people.In the liquid submerged fermentation process of edible medicinal fungus bacterial classification, it is particularly important that the first-generation produces female making of planting, it directly affects the quality of bacterial classification in the subsequent handling of liquid submerged fermentation, and produce for cultivating the first-generation success or failure that female substratum of planting determines the liquid submerged fermentation of edible medicinal fungus bacterial classification especially, therefore, research and develop and be a kind ofly applicable to make the edible medicinal fungus first-generation to produce female substratum of planting very important.
Summary of the invention:
First object of the present invention is to provide an a kind of edible medicinal fungus generation to produce the collocation method of female high-quality substratum of planting.
Second object of the present invention is the substratum providing a kind of collocation method utilizing an edible medicinal fungus generation to produce female high-quality substratum of planting obtained.
First object of the present invention is implemented by following technical scheme: an edible medicinal fungus generation produces female high-quality substratum compound method of planting, the preparation of it comprises the steps: (1) wood chip liquid; (2) component weighs and preparation; (3) packing sterilizing; Wherein,
(1) preparation of wood chip liquid: get wood chip and be soaked in 4-6h in water, the mass ratio of described wood chip and described water is 1:1.2-1.3, is then placed on heating installation and boils 40min, i.e. obtained wood chip juice;
(2) component weighs and preparation: take PAD substratum 12.5-25g, described wood chip juice 37.5-75g, magnesium sulfate heptahydrate 0.5g, ammonium sulfate lg, 0.25ppm myricyl alcohol 250mL, and add water and be configured to the solution of 1000mL;
(3) packing sterilizing: the solution configured is carried out packing sterilizing, the pressure of described sterilizing is 1.0MPa, temperature is 121 DEG C, the time is 20-30min, obtained substratum.
Preferably, described wood chip is for being ground into 80-100 object white birch and toothed oak trees bits, and it is that the ratio of 1:1 mixes that described white birch wood chip and described toothed oak trees are considered to be worth doing with mass ratio.
Second object of the present invention is implemented by following technical scheme: the substratum that the high-quality substratum compound method utilizing an edible medicinal fungus generation to produce female kind is prepared from.
Advantage of the present invention: l, substratum of the present invention can promote mycelial growth, have virus removal effect, prevent miscellaneous bacteria from producing, and can not produce latent virus and miscellaneous bacteria, and then promote the quality and the quality that improve product; The mycelium of 2, turning out pure white sturdy, to grow intensive, neat in edge, full packages time shorter; 3, promote sporophore growth, and then improve its output.
Accompanying drawing illustrates:
Fig. 1 is embodiment 4 experimental group mycelia.
Fig. 2 is embodiment 4 control group mycelia.
Fig. 3 is embodiment 4 experimental group sporophore.
Fig. 4 is embodiment 4 control group sporophore.
Embodiment:
Embodiment 1:
An edible medicinal fungus generation produces female high-quality substratum compound method of planting, the preparation of it comprises the steps: (1) wood chip liquid; (2) component weighs and preparation; (3) packing sterilizing; Wherein,
(1) preparation of wood chip liquid: get wood chip and be soaked in 4h in water, wood chip is for being ground into 80 object white birchs and toothed oak trees bits, it is 1:1 that white birch wood chip and toothed oak trees are considered to be worth doing with mass ratio, white birch growth extends region from root stream gold neck, the growth of toothed oak tree is from A Bend in the River town, Zhalantun north slope, the mass ratio of wood chip and water is 1:1.2, is then placed on heating installation and boils 40min, i.e. obtained wood chip juice;
(2) component weighs and preparation: take PAD substratum 12.5g, wood chip juice 37.5g, magnesium sulfate heptahydrate 0.5g, ammonium sulfate lg, 0.25ppm myricyl alcohol 250mL, and add water and be configured to the solution of 1000mL;
(3) packing sterilizing: the solution configured is carried out packing sterilizing, the pressure of sterilizing is 1.0MPa, temperature is 121 DEG C, the time is 20min, obtained substratum.
Embodiment 2:
An edible medicinal fungus generation produces female high-quality substratum compound method of planting, the preparation of it comprises the steps: (1) wood chip liquid; (2) component weighs and preparation; (3) packing sterilizing; Wherein,
(1) preparation of wood chip liquid: get wood chip and be soaked in 6h in water, wood chip is for being ground into 100 object white birchs and toothed oak trees bits, it is 1:1 that white birch wood chip and toothed oak trees are considered to be worth doing with mass ratio, white birch growth extends region from root stream gold neck, the growth of toothed oak tree is from A Bend in the River town, Zhalantun north slope, the mass ratio of wood chip and water is 1:1.25, is then placed on heating installation and boils 40min, i.e. obtained wood chip juice;
(2) component weighs and preparation: take PAD substratum 20g, wood chip juice 60g, magnesium sulfate heptahydrate 0.5g, ammonium sulfate lg, 0.25ppm myricyl alcohol 250mL, and add water and be configured to the solution of 1000mL;
(3) packing sterilizing: the solution configured is carried out packing sterilizing, the pressure of sterilizing is 1.0MPa, temperature is 121 DEG C, the time is 30min, obtained substratum.
Embodiment 3:
An edible medicinal fungus generation produces female high-quality substratum compound method of planting, the preparation of it comprises the steps: (1) wood chip liquid; (2) component weighs and preparation; (3) packing sterilizing; Wherein,
(1) preparation of wood chip liquid: get wood chip and be soaked in 5h in water, wood chip is for being ground into 90 object white birchs and toothed oak trees bits, it is 1:1 that white birch wood chip and toothed oak trees are considered to be worth doing with mass ratio, white birch growth extends region from root stream gold neck, the growth of toothed oak tree is from A Bend in the River town, Zhalantun north slope, the mass ratio of wood chip and water is 1:1.3, is then placed on heating installation and boils 40min, i.e. obtained wood chip juice;
(2) component weighs and preparation: take PAD substratum 25g, wood chip juice 75g, magnesium sulfate heptahydrate 0.5g, ammonium sulfate lg, 0.25ppm myricyl alcohol 250mL, and add water and be configured to the solution of 1000mL;
(3) packing sterilizing: the solution configured is carried out packing sterilizing, the pressure of sterilizing is 1.0MPa, temperature is 121 DEG C, the time is 20-30min, obtained substratum.
Embodiment 4:
According to the substratum that the method for embodiment 1 or 2 or 3 is prepared from, the test called after experimental group below utilizing the present embodiment substratum to carry out, the test called after control group utilizing PDA substratum to carry out;
Under the same test conditions, utilize the present embodiment substratum and PAD culture medium culturing mycelia, the speed of growth of mycelia in two kinds of substratum is as shown in table 1.
The speed of growth of table 1 mycelia in two kinds of substratum
As shown in Table 1, experimental group Growth rate is 10.78mm/d, control group mycelial growth rate is 8.15mm/d, the strong formula that Patent media of the present invention is described, accelerating the speed of growth to mycelium has promoter action, and then virus and varied bacteria growing can be suppressed, there is anti-latent virus, play bacterial classification detoxification, impel thus and improve product production and quality, accomplishing should new normality, and saving of work and time is good and fast to be increased production again.
Fig. 1 and Fig. 2 is under the same test conditions, utilizes the present embodiment substratum and PAD culture medium culturing mycelia.From Fig. 1 and Fig. 2, the experimental group mycelium of turning out pure white sturdy, to grow intensive, neat in edge, full packages time shorter, contrast with experimental group, control group mycelium color be partially yellow, mycelium is comparatively thin, it is sparse to grow, edge is uneven, the full packages time is longer.
Fig. 3 and Fig. 4 is under the same test conditions, utilizes the sporophore that the present embodiment substratum and PAD culture medium culturing go out respectively.From Fig. 3 and Fig. 4, the sporophore that experimental group is turned out is pure white sturdy, and the sporophore that control group is turned out comparatively experimental group color is partially yellow, sporophore is partially thin, illustrates that the present embodiment can promote the growth of sporophore, and then raising fruiting body yield.
Claims (3)
1. an edible medicinal fungus generation produces female high-quality substratum compound method of planting, and it is characterized in that, the preparation of it comprises the steps: (1) wood chip liquid; (2) component weighs and preparation; (3) packing sterilizing; Wherein,
(1) preparation of wood chip liquid: get wood chip and be soaked in 4-6h in water, the mass ratio of described wood chip and described water is 1:1.2-1.3, is then placed on heating installation and boils 40min, i.e. obtained wood chip juice;
(2) component weighs and preparation: take PDA substratum 12.5-25g, described wood chip juice 37.5-75g, magnesium sulfate heptahydrate 0.5g, ammonium sulfate lg, 0.25ppm myricyl alcohol 250mL, and add water and be configured to the solution of 1000mL;
(3) packing sterilizing: the solution configured is carried out packing sterilizing, sterilization pressure is 1.0MPa, temperature is 121 DEG C, the time is 25-30min, obtained substratum.
2. an edible medicinal fungus generation according to claim 1 produces female high-quality substratum compound method of planting, it is characterized in that, described wood chip is for being ground into 80-100 object white birch wood chip and toothed oak trees bits, and it is that the ratio of 1:1 mixes that described white birch wood chip and described toothed oak trees are considered to be worth doing with mass ratio.
3. the substratum utilizing the female high-quality substratum compound method of planting of the production of the edible medicinal fungus generation described in claim 1 or 2 to be prepared from.
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| CN104285663A (en) * | 2013-07-16 | 2015-01-21 | 曲淑敏 | Black termitomyces cultivation method |
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| CN101368158A (en) * | 2008-10-08 | 2009-02-18 | 马宏达 | Preparation method for birch fuscoporia ferrugineo-fusca teng submerged fermentation culture medium |
| CN101368159A (en) * | 2008-10-08 | 2009-02-18 | 马宏达 | Preparation method for inonotus obliquus mother seed culture medium |
| CN101423800A (en) * | 2008-11-13 | 2009-05-06 | 广东省微生物研究所 | Cultivation method of fungus hexagonia apiaria fruit body |
| CN103304298A (en) * | 2013-06-02 | 2013-09-18 | 邬金飞 | Hericium erinaceus mother strain culture medium and making method thereof |
| CN104285663A (en) * | 2013-07-16 | 2015-01-21 | 曲淑敏 | Black termitomyces cultivation method |
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Application publication date: 20151209 |