CN101368158A - Preparation method for birch fuscoporia ferrugineo-fusca teng submerged fermentation culture medium - Google Patents

Preparation method for birch fuscoporia ferrugineo-fusca teng submerged fermentation culture medium Download PDF

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Publication number
CN101368158A
CN101368158A CNA2008101372650A CN200810137265A CN101368158A CN 101368158 A CN101368158 A CN 101368158A CN A2008101372650 A CNA2008101372650 A CN A2008101372650A CN 200810137265 A CN200810137265 A CN 200810137265A CN 101368158 A CN101368158 A CN 101368158A
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wheat bran
submerged fermentation
birch
percent
peptone
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CNA2008101372650A
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马宏达
康可新
许修宏
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马宏达
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Abstract

The invention discloses a method for preparing liquid medium of medicinal fungus inonotus obliquus submerged fermentation mycelia. The liquid medium is made by the raw materials, such as birch sawdust, wheat bran, glucose, maltose, peptone, vitamin B1, yeast extract, KH2PO and MgSO4. The weight percentage of the materials is as follows: 10 to 20 percent of glucose, 0 to 20 percent of maltose, 0 to 5 percent of peptone, 0 to 5 percent of yeast extract, 0 to 0.5 percent of KH2PO, 0 to 0.3 percent of MgSO4, 40 to 80 percent of birch sawdust, and 10 to 30 percent of wheat bran. The formulating method of the invention includes a preparation method of birch sawdust juice, a preparation method of wheat bran juice and a mixing method of various raw materials. The medium prepared from the method is used for inonotus obliquus, each component of the mycelium obtained from submerged fermentation of the inonotus obliquus is essentially consistent with that of the sclerotium of wild inonotus obliquus, and the content of sterols functional component is much higher than that of sclerotium of wild inonotus obliquus.

Description

The preparation method of birch fuscoporia ferrugineo-fusca teng submerged fermentation culture medium
Technical field
The present invention relates to a kind of preparation method of submerged fermentation culture medium of medicinal fungi Phaeopoms obliquus, especially with birch bits, wheat bran, glucose, maltose, peptone, yeast extract paste, KH 2PO, MgSO 4, vitamins B 1Preparation for the liquid nutrient medium of raw material.
Background technology
Phaeopoms obliquus [Inonotus obliguus (Fr) Pilat] is that a kind of application will be used fungi very widely on Russia, Poland, Finland and other places, English name chaga.Nineteen fifty-five, chaga is used for treating tumour, particularly Digestive tract and lung tumors by Russian Medicine Research Inst..16th century, the among the people of some countries of Eastern Europe prevented curing cancers with regard to the sclerotium with this bacterium so far.This fungi of Khanty human of western Leah prevents and treats heart trouble, hepatopathy, stomach trouble and esophagus disease etc.Recent research shows, Phaeopoms obliquus can suppress hiv virus (HIV-1), radioprotective and by the biosynthesizing of arrestin matter, antimitotic and eliminate that mechanism of action such as free radical activity suppresses or the growth and the control 0-157 intestinal bacteria that delay tumour cell poison.
The use of Phaeopoms obliquus is mainly based on wild, wild Phaeopoms obliquus mainly be grown in area, Muscovite siberian, Hokkaido, Japan, Finland, etc. ground.There is wild Phaeopoms obliquus to grow in China Changbai mountain, Jilin area and Zhang Guangcai mountain range, Heilungkiang and area, large and small Xing'an Mountains.
Biological study shows that Phaeopoms obliquus is grown very slow under wild state, wild kind is only grown on the birch that lives and just had very high pharmaceutical use in 10~15 years, and therefore wild Phaeopoms obliquus price is very expensive.The U.S. also classifies it as " special natural goods " following drink as the extraterrestrial.Therefore, the research of Phaeopoms obliquus and Products Development have very wide application prospect.Because rare wild resource does not satisfy the growing market requirement far away, from the Humfeld of U.S. H etc. the submerged fermentation of mushroom is studied, and after proposing to cultivate the mycelium of mushroom with fermentation method, investigators utilize this method to carry out the fermentation of multiple mushroom.Submerged fermentation have the cycle short, output is high, opportunities for contamination is little, not limited by surrounding environment, resource apparatus utilization ratio advantages of higher.Preparing the Phaeopoms obliquus mycelium with fermentation method, to alleviate the deficiency of wild resource be practicable, and do not appear in the newspapers as yet at the mass-producing submerged fermentation of Phaeopoms obliquus.
The use of birch fuscoporia ferrugineo-fusca teng submerged fermentation culture medium of the present invention can make each nutritive ingredient of mycelium of the Phaeopoms obliquus that obtains by submerged fermentation and the nutritive ingredient basically identical in the wild inonotus obliquus sclerotium, and content exceeds much in the sclerotium of the wilder Phaeopoms obliquus of content of sterols functional component, and actual application value is arranged very much.
Summary of the invention
The objective of the invention is to, with selected raw materials of glucose, maltose, peptone, yeast extract paste, KH 2PO, MgSO 4, vitamins B 1With make birch wood chip liquor by special methods and combine in proportion with the wheat bran liquor and make the substratum that is used for birch fuscoporia ferrugineo-fusca teng submerged fermentation.
Substratum of the present invention is achieved in that
Birch bits, wheat bran, glucose, maltose, peptone, yeast extract paste, KH 2PO, MgSO 4, vitamins B 1Its weight percent is respectively: glucose 10~20%, maltose 0~20%, peptone 0~5%, yeast extract paste 0~5%, KH 2PO0~0.5%, MgSO 40~0.3%, birch bits 40~80%, wheat bran 10~30%.
The preparation method of substratum is: with birch bits, wheat bran difference liquor, get filtrate, be sequentially added into glucose, maltose, peptone, yeast extract paste, KH 2PO, MgSO 4, vitamins B 1, to boil the material of adding is fully dissolved, sterilization makes and is used for birch fuscoporia ferrugineo-fusca teng submerged fermentation and cultivates mycelial liquid nutrient medium.The production stage of making substratum is as follows:
1. the preparation of birch bits juice: get do not have go mouldy, the exsiccant birch considered 40~80 mesh sieves to be worth doing, took by weighing in proportion, poured in 4~10 times the boiling water and was stirred to boiling back 30~70min, crossed leaching filtrate.
2. the preparation of wheat bran juice: get do not have go mouldy, dry wheat bran sieve dust removal soil, put into 4~6 times clear water, constantly stir and boil 15~40min.Cross leaching filtrate.
3. the mixing of the nutritive substance of substratum: get 1 and 2 filtrates that make and mix, boil, add glucose, maltose, peptone, yeast extract paste, KH respectively in proportion 2PO, MgSO 4, vitamins B 1, fully stir and boil, nutritive substance can fully be dissolved in the boiling water, form the nutrient solution of stable homogeneous.The nutrient solution that makes is poured in the culture tank into sterilization.Make the substratum that is used for the submerged fermentation of Phaeopoms obliquus mycelia.
Advantage of the present invention is:
The present invention has realized can be used for the preparation of the substratum that wild Phaeopoms obliquus mycelia submerged fermentation cultivates, and the material choice of this substratum is reasonable, and with low cost, be easy to get.Composition basically identical in each composition of mycelium that the liquid nutrient medium fermentation of using the present invention to make makes and the wild inonotus obliquus sclerotium, content exceeds much in the wilder inonotus obliquus sclerotium of sterols functional component content.

Claims (2)

1. one kind is used for the substratum that the submerged fermentation of medicinal fungi Phaeopoms obliquus mycelium is cultivated, and it is characterized in that with birch bits, wheat bran, glucose, maltose, peptone, yeast extract paste, KH 2PO, MgSO 4, vitamins B 1Be raw material, its weight percent is respectively: glucose 10~20%, maltose 0~20%, peptone 0~5%, yeast extract paste 0~5%, KH 2PO 0~0.5%, MgSO 40~0.3%, birch bits 40~80%, wheat bran 10~30%.
2. the preparation method of Phaeopoms obliquus mycelium submerged fermentation culture medium according to claim 1, the making method of birch bits juice, the preparation method of wheat bran juice, glucose, maltose, peptone, yeast extract paste, KH 2PO, MgSO 4, vitamins B 1Each raw material is mixed in proportion sterilization and makes liquid nutrient medium.The step of making submerged fermentation culture medium is as follows:
1) preparation of birch bits juice: get do not have go mouldy, the exsiccant birch considered 40~80 mesh sieves to be worth doing, took by weighing in proportion, poured in 4~10 times the boiling water and was stirred to boiling back 30~70min, crossed leaching filtrate.
2) preparation of wheat bran juice: get do not have go mouldy, dry wheat bran sieve dust removal soil, put into 4~6 times clear water, constantly stir and boil 15~40min.Cross leaching filtrate.
3) mixing of the nutritive substance of substratum: get 1 and 2 filtrates that make and mix, boil, add glucose, maltose, peptone, yeast extract paste, KH respectively in proportion 2PO, MgSO 4, vitamins B 1, fully stir and boil, nutritive substance can fully be dissolved in the boiling water, form the nutrient solution of stable homogeneous.The nutrient solution that makes is poured in the culture tank into sterilization.Make the substratum that is used for the submerged fermentation of Phaeopoms obliquus mycelia.
CNA2008101372650A 2008-10-08 2008-10-08 Preparation method for birch fuscoporia ferrugineo-fusca teng submerged fermentation culture medium Pending CN101368158A (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101622939B (en) * 2009-08-03 2011-04-20 天津科技大学 Inonotus obliquus deep culture method
CN102219590A (en) * 2011-05-13 2011-10-19 三明市三真生物科技有限公司 Culture medium and culture method of Fuscoporia punctata first level strain
CN103146775A (en) * 2011-12-07 2013-06-12 徐向群 Method for preparing epigallocatechin-3-gallate and epigallocatechin gallate by liquid-submerged fermentation
CN105130594A (en) * 2015-08-10 2015-12-09 呼伦贝尔生态产业技术研究院 Preparation method of high quality medium for edible-medicinal fungus first generation production mother culture and medium
TWI601818B (en) * 2016-12-30 2017-10-11 天明製藥股份有限公司 Medium of solid fermentation for inonotus obliquus and preparation method thereof
CN108531402A (en) * 2017-03-03 2018-09-14 天明制药股份有限公司 Liquid culture medium of inonotus obliquus and preparation method thereof
WO2021017449A1 (en) * 2019-07-29 2021-02-04 浙江养生堂天然药物研究所有限公司 Fermented brich juice, production method therefor and use thereof in composition for external application to skin

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101622939B (en) * 2009-08-03 2011-04-20 天津科技大学 Inonotus obliquus deep culture method
CN102219590A (en) * 2011-05-13 2011-10-19 三明市三真生物科技有限公司 Culture medium and culture method of Fuscoporia punctata first level strain
CN103146775A (en) * 2011-12-07 2013-06-12 徐向群 Method for preparing epigallocatechin-3-gallate and epigallocatechin gallate by liquid-submerged fermentation
CN105130594A (en) * 2015-08-10 2015-12-09 呼伦贝尔生态产业技术研究院 Preparation method of high quality medium for edible-medicinal fungus first generation production mother culture and medium
TWI601818B (en) * 2016-12-30 2017-10-11 天明製藥股份有限公司 Medium of solid fermentation for inonotus obliquus and preparation method thereof
CN108531402A (en) * 2017-03-03 2018-09-14 天明制药股份有限公司 Liquid culture medium of inonotus obliquus and preparation method thereof
WO2021017449A1 (en) * 2019-07-29 2021-02-04 浙江养生堂天然药物研究所有限公司 Fermented brich juice, production method therefor and use thereof in composition for external application to skin

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