CN102405768A

CN102405768A - Method for acclimating mother seeds of hypsizygus marmoreus

Info

Publication number: CN102405768A
Application number: CN2011102625352A
Authority: CN
Inventors: 盛岩; 李波; 李凤玲; 张金霞; 张红霞; 荣艳君; 曲文成; 王艳霞; 蔡王涛; 董孝刚
Original assignee: SHANDONG ZHENGHAN BIOTECHNOLOGY GROUP CO Ltd
Current assignee: SHANDONG ZHENGHAN BIOTECHNOLOGY GROUP CO Ltd
Priority date: 2011-08-31
Filing date: 2011-08-31
Publication date: 2012-04-11

Abstract

A method for acclimating mother seeds of hypsizygus marmoreus comprises the following steps of adopting a better mother seed tube to activate; inoculating on 10-15 agar (barren) plates; after breeding, inoculating the strains which are optimally screened on a PDA plate; after breeding, optimally inoculating the plate in a PDA tube; after breeding, optimally inoculating to the PDA tube; and adopting the best one as the mother seed to store. The invention has the following beneficial effects: fast growth speed of the hypha, short growth period, early fruiting time, strong ability for resisting microorganisms and good quality.

Description

The female domesticating method of planting of Hypsizygus marmoreus

Technical field

The present invention relates to the female domesticating method of planting of a kind of Hypsizygus marmoreus.

Background technology

Hypsizygus marmoreus is a kind of nutritious edible medicinal rare famous and precious edible mushroom of holding concurrently, and the hot water of its fruit body and extractive with organic solvent have removes interior free yl, raising body immunity, the old and feeble effect of prevention.At present, the commercialization of Hypsizygus marmoreus plantation is one of important kind of edible fungus industrial production.But because the life cycle of bacterial classification is long, bacterial classification is prone to take place aging, aging mycelial growth point less, vigor a little less than, thereby the Hypsizygus marmoreus of producing the anti-bacterium ability of mixing can take place a little less than, phenomenons such as poor stability, growth vigor reduction, poor quality and output reduction.Strain domestication is meant through the artificial measures and makes edible mushroom progressively adapt to a certain condition, and the method for directive breeding edible mushroom can obtain the bacterial strain with higher tolerance and mobility through domestication.Present most of culturing edible fungus or bacterial strain all are to obtain through the domestication, and the female domesticating method of planting of Hypsizygus marmoreus does not also occur.

Summary of the invention

It is short to the purpose of this invention is to provide a kind of growth cycle, improves the female domesticating method of planting of Hypsizygus marmoreus of bacterial classification quality.

The present invention realizes through following method:

Select a more excellent mother and plant test tube and carry out activation, the cycle is 1～3 day, is connected on about 10～15 water agar (barren) flat board; After cultivating 10 days under 20 ℃～25 ℃ conditions of constant incubator, select no corner disappearance, the flourishing flat board of mycelia to preserve other dull and stereotyped eliminating; Mortality is connected to the bacterial classification that filters out on the PDA flat board more than 80%, after cultivating 10 days under 180～26 ℃ of conditions of constant incubator; Select no corner disappearance, substrate mycelium flat board flourishing, the growth unanimity to preserve, other dull and stereotyped eliminating, mortality is more than 80%; Flat board is connected in the PDA test tube; Under 18 ℃～26 ℃ conditions of constant incubator, cultivated 10 days, select no corner disappearance, flourishing, the consistent test tube of growth of substrate mycelium, get its front end; Be forwarded to again in the PDA test tube, select a wherein best female kind of the conduct preservation of performance.

The invention has the beneficial effects as follows:

1, mycelial growth rate is fast, and growth cycle is short, and the fruiting time, early culture period reduced 10～20 days;

2, high yield is tamed through female the kind, and PR is domesticated strain raising the output 50%～80% not;

3, anti-assorted bacterium power is stronger, when meeting assorted bacterium such as Mucor, green mold can with its competitive growth, and cover the bacterium of mixing;

4, the Hypsizygus marmoreus active component content of output is high, quality better;

5, fruiting is neat.

Embodiment

Below in conjunction with embodiment the present invention is further specified.

Embodiment 1:

Select a more excellent mother and plant test tube and carry out activation, the cycle is 3 days, is connected on about 15 water agar plates; After cultivating 10 days under 20 ℃ of-25 ℃ of conditions of constant incubator; Select no corner disappearance, the flourishing flat board of substrate mycelium to preserve, other dull and stereotyped eliminating, mortality is more than 80%.The bacterial classification that filters out is connected on the PDA flat board, after cultivating 10 days under 20 ℃ of-25 ℃ of conditions of constant incubator, selects no corner disappearance, substrate mycelium flat board flourishing, the growth unanimity to preserve, other dull and stereotyped eliminating, mortality is more than 80%.Be connected in the PDA test tube (barren), under 20 ℃ of-25 ℃ of conditions of constant incubator, cultivated 10 days.Select no corner disappearance, prosperity of substrate mycelium, the consistent test tube of growth, get its front end, be forwarded to again in the PDA test tube, select a wherein best female kind of the conduct preservation of performance.

Embodiment 2:

Selecting a more excellent mother plants test tube and carries out activation; Cycle is 2 days; Be connected on about 12 water agar (barren) flat board, after cultivating 10 days under 20 ℃ of-25 ℃ of conditions of constant incubator, select no corner disappearance, the flourishing flat board of substrate mycelium to preserve; Other dull and stereotyped eliminating, mortality is more than 80%.The bacterial classification that filters out is connected on the more rich PDA flat board of nutrition; After cultivating 10 days under 20 ℃ of-25 ℃ of conditions of constant incubator; Select no corner disappearance, substrate mycelium flat board flourishing, the growth unanimity to preserve, other dull and stereotyped eliminating, mortality is more than 80%.Be connected in the PDA test tube, under 20 ℃ of-25 ℃ of conditions of constant incubator, cultivated 10 days.Get its front end, be forwarded to again in the PDA test tube, select a wherein best female kind of the conduct preservation of performance.

Claims

1. the female domesticating method of planting of a Hypsizygus marmoreus is characterized in that: select a more excellent mother and plant test tube and carry out activation, the cycle is 1～3 day; Be connected on about 10～15 water agar (barren) flat board, after cultivating 10 days under 20 ℃～25 ℃ conditions of constant incubator, select no corner disappearance, the flourishing flat board of mycelia to preserve; Other dull and stereotyped eliminating, mortality is connected to the bacterial classification that filters out on the PDA flat board more than 80%; After cultivating 10 days under 18 ℃～26 ℃ conditions of constant incubator, select no corner disappearance, substrate mycelium flat board flourishing, the growth unanimity to preserve other dull and stereotyped eliminating; Mortality is connected to flat board in the PDA test tube more than 80%, under 18 ℃～26 ℃ conditions of constant incubator, cultivates 10 days; Select no corner disappearance, flourishing, the consistent test tube of growth of substrate mycelium; Get its front end, be forwarded to again in the PDA test tube, select a wherein best female kind of the conduct preservation of performance.