CN101288381A - Collective medium for flower tissue culture - Google Patents
Collective medium for flower tissue culture Download PDFInfo
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- CN101288381A CN101288381A CNA2007100571633A CN200710057163A CN101288381A CN 101288381 A CN101288381 A CN 101288381A CN A2007100571633 A CNA2007100571633 A CN A2007100571633A CN 200710057163 A CN200710057163 A CN 200710057163A CN 101288381 A CN101288381 A CN 101288381A
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Abstract
The invention discloses a collective medium of tissue culture of flower, and the collective medium is adopted in the tissue culture of flower for proliferation and subculture of test-tube plantlet. The collective medium avoids the process that the medium is separately made of various breeds in tissue culture of flower, is simple and easy to operate, and can effectively reduce the process cost, thus having extensive application value and prospect.
Description
Technical field
The invention belongs to agricultural biological technical field, relate to the used medium of a kind of flowers tissue culture, especially relate to a kind of flowers tissue culture collective media.
Background technology
Plant tissue culture technique is the new technology that modern age, bioscience grew up, and is the chief component of biotechnology (being biotechnology), is the large-scale production of carrying out in the cultivating chamber that fast numerous detoxification is batch production in the group training chamber of ultra-clean.The totipotency of plant cell was proposed from Harbrant (G.Haberlandt) in 1902, be this cell of each plant or a complete set of gene that sexual cell all has this plant, therefore each cell all can develop into a plant the same with parent under certain condition of culture.Though this notion proposed at the beginning of last century, under technical conditions at that time, in practice, do not accomplish.Through the effort of countless scientists over more than 80 years, tissue culture technique is updated, and it is perfect, ripe that technology is tending towards, and the totipotency of cell not only is proved in theory fully at present, and is that the application of tissue culture in practice laid a good foundation.Plant Tissue Breeding is in full flourish, makes much progress all over the world.
Over nearly 40 years, tissue culture technique has become one of indispensable means in the production of flowers and plants, and aspects such as flower plant rearing new variety, kind detoxification, purification and rejuvenation, fast asexual propagation, the production of expansion seedling have all been produced epoch-making influence.The existing hundreds of above flowers kind tissue culture of China are succeedd.But up to the present, people adopt separately independently medium to the tissue culture of all kinds of flower varieties more, and the collective media that is used for the flowers tissue culture is rarely found.
Summary of the invention
Need distinguish the complex process of making medium separately in order to solve in the flowers tissue culture each flower variety, the invention provides a kind of flowers tissue culture collective media.
The present invention selects suitable hormone and proportioning thereof for use dexterously according to the principle of " advanced, suitable, economy ", provides a kind of flowers tissue culture collective media, to satisfy the technical performance requirement of flowers tissue culture.
A kind of flowers tissue culture of the present invention collective media mainly comprises minimal medium and supplementary element, it is characterized in that: minimal medium adopts the MS medium, and supplementary element is made up of hormone, sugar and curing agent.Wherein hormone comprises two kinds in the basic element of cell division and growth hormone.Adopt BA (6-benzyl aminopurine), KT (kinetin) as the basic element of cell division respectively, its component content is good with 1mg/L all; IBA (indolebutyric acid) is as growth hormone, and its component content is good with 0.5mg/L.Carbohydrate adopts soft white sugar, and its component content is 3%.Curing agent adopts agar powder, and its component content is 0.45%.
Flowers of the present invention are polyploid tawny daylily, yellow people from sky chrysanthemum, inferior chrysanthemum.The present invention is cut apart the bud of growing thickly of polyploid tawny daylily, yellow people from sky chrysanthemum, inferior each flower variety of chrysanthemum by the rip cutting of single bud seedling, place on the collective media and cultivate, and cuts apart subculture once about per 4 weeks, and budlet incessantly in a large number all can be grown thickly.
The invention has the beneficial effects as follows:
1. the test-tube plantlet proliferation and subculture medium of each kind can be general in the flowers tissue culture, and it is easy to reach operation sequence.
2. each flower variety in the flowers tissue culture need be distinguished the complicated production operation of making medium separately and simplify, the technology cost reduces, and economic benefit increases.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail: but the present invention is not limited to following embodiment.
Embodiment 1
Press such scheme, the test-tube plantlet of good luck tawny daylily, yellow day people chrysanthemum, inferior chrysanthemum three kinds is seeded in respectively on the present invention's the collective media, subculture add up after 30 days and is bred the result.And with code name be " 1065
#, 2117
#, 2118
#, " three kinds of non-collective medias compare test-tube plantlet breeding rate on each medium.
Table
1Collective media and non-collective media shoot proliferation effect are relatively
Result of implementation is as shown in table 1:
1. good luck tawny daylily, yellow day people chrysanthemum and the breeding rate of each kind test-tube plantlet of inferior chrysanthemum in the present invention's collective media are respectively 3.84,3.67,2.67.Its test-tube plantlet proliferation and subculture culture effect (this non-collective media of breeding rate " 1065 " in inferior chrysanthemum test-tube plantlet is all outside 2.67) all is better than other non-collective media.
2. " 1065
#, 2117
#, 2118
#" three kinds of non-collective medias show because of flower variety is different in the test-tube plantlet proliferation and subculture of each flower variety and differ, promptly each medium can only be adapted to its corresponding flower variety, can not be general.
Embodiment 2
Press such scheme, " X505-5, X504-6, LX508 " three test-tube plantlet clones of good luck tawny daylily kind are seeded in the present invention's collective media and non-collective media (2117 respectively
#) on.
The implementation result (table 2) that subculture obtained after 30 days is: the test-tube plantlet proliferation and subculture culture effect of the present invention's collective media in three test-tube plantlet clones of good luck tawny daylily " X505-5, X504-6, LX508 " all is better than non-collective media.
Table 2 good luck tawny daylily shoot proliferation culture effect
In sum, the present invention's medium has distinctive feature, the test-tube plantlet proliferation and subculture medium of each kind can be general in the flowers tissue culture, not only its proliferation and subculture effect is remarkable, and simplified operation sequence, reduced cost, practical, have broad application prospects and market potential.
Claims (5)
1, a kind of flowers tissue culture collective media is characterized in that the composition of this collective media is as follows:
Minimal medium: MS medium.
Supplementary element: BA (6-benzyl aminopurine), KT (kinetin), IBA (indolebutyric acid), sugar, agar powder.
2, flowers tissue culture collective media according to claim 1 is characterized in that each component content of described flowers tissue culture collective media is:
MS+BA 1mg/L+KT 1mg/L+IBA0.5mg/L+ soft white sugar 3%+ agar powder 0.45%.
3, the described flowers tissue culture of claim 1 collective media is characterized in that described collective media PH=5.8-6.0.
4, the described flowers tissue culture of claim 1 collective media is characterized in that described collective media is a test-tube plantlet proliferation and subculture collective media.
5, the described flowers tissue culture of claim 4 collective media is characterized in that described flowers are polyploid tawny daylily, yellow people from sky chrysanthemum, inferior chrysanthemum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100571633A CN101288381A (en) | 2007-04-18 | 2007-04-18 | Collective medium for flower tissue culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100571633A CN101288381A (en) | 2007-04-18 | 2007-04-18 | Collective medium for flower tissue culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101288381A true CN101288381A (en) | 2008-10-22 |
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|---|---|---|---|
| CNA2007100571633A Pending CN101288381A (en) | 2007-04-18 | 2007-04-18 | Collective medium for flower tissue culture |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104285811A (en) * | 2014-10-24 | 2015-01-21 | 柳州市天姿园艺有限公司 | Special culture medium for chrysanthemum lavandulifolium |
| CN104285808A (en) * | 2014-10-24 | 2015-01-21 | 柳州市天姿园艺有限公司 | Special culture medium for cut chrysanthemum |
| CN104285770A (en) * | 2014-10-24 | 2015-01-21 | 柳州市天姿园艺有限公司 | Special-purposed culture medium for dimorphotheca sinuata |
| CN104285812A (en) * | 2014-10-24 | 2015-01-21 | 柳州市天姿园艺有限公司 | Special culture medium for sneezeweed |
| CN104304024A (en) * | 2014-10-24 | 2015-01-28 | 柳州市天姿园艺有限公司 | Special culture medium for chrysanthemum zawadskii herbich |
| CN104304022A (en) * | 2014-10-24 | 2015-01-28 | 柳州市天姿园艺有限公司 | Special culture medium for fertilized chrysanthemum |
| CN104304021A (en) * | 2014-10-24 | 2015-01-28 | 柳州市天姿园艺有限公司 | Special culture medium for ise-chrysanthemum |
| CN104304023A (en) * | 2014-10-24 | 2015-01-28 | 柳州市天姿园艺有限公司 | Special culture medium for ground-cover chrysanthemum |
| CN104351049A (en) * | 2014-10-24 | 2015-02-18 | 柳州市天姿园艺有限公司 | Special culture medium for cineraria |
| CN104351050A (en) * | 2014-10-24 | 2015-02-18 | 柳州市天姿园艺有限公司 | Special culture medium for chrysanthemum chanetii |
| CN104396740A (en) * | 2014-10-24 | 2015-03-11 | 柳州市天姿园艺有限公司 | Chrysanthemum nankingense special-purpose medium |
| CN104396739A (en) * | 2014-10-24 | 2015-03-11 | 柳州市天姿园艺有限公司 | Dendranthema vestitum special culture medium |
| CN107455391A (en) * | 2017-08-15 | 2017-12-12 | 河南省农业科学院园艺研究所 | A kind of grafting activator and the method for improving graft survival rate |
| CN109329052A (en) * | 2018-10-09 | 2019-02-15 | 中国中医科学院中药研究所 | Aristolochia contorta tissue-cultured seedling expanding propagation method |
-
2007
- 2007-04-18 CN CNA2007100571633A patent/CN101288381A/en active Pending
Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104285811A (en) * | 2014-10-24 | 2015-01-21 | 柳州市天姿园艺有限公司 | Special culture medium for chrysanthemum lavandulifolium |
| CN104285808A (en) * | 2014-10-24 | 2015-01-21 | 柳州市天姿园艺有限公司 | Special culture medium for cut chrysanthemum |
| CN104285770A (en) * | 2014-10-24 | 2015-01-21 | 柳州市天姿园艺有限公司 | Special-purposed culture medium for dimorphotheca sinuata |
| CN104285812A (en) * | 2014-10-24 | 2015-01-21 | 柳州市天姿园艺有限公司 | Special culture medium for sneezeweed |
| CN104304024A (en) * | 2014-10-24 | 2015-01-28 | 柳州市天姿园艺有限公司 | Special culture medium for chrysanthemum zawadskii herbich |
| CN104304022A (en) * | 2014-10-24 | 2015-01-28 | 柳州市天姿园艺有限公司 | Special culture medium for fertilized chrysanthemum |
| CN104304021A (en) * | 2014-10-24 | 2015-01-28 | 柳州市天姿园艺有限公司 | Special culture medium for ise-chrysanthemum |
| CN104304023A (en) * | 2014-10-24 | 2015-01-28 | 柳州市天姿园艺有限公司 | Special culture medium for ground-cover chrysanthemum |
| CN104351049A (en) * | 2014-10-24 | 2015-02-18 | 柳州市天姿园艺有限公司 | Special culture medium for cineraria |
| CN104351050A (en) * | 2014-10-24 | 2015-02-18 | 柳州市天姿园艺有限公司 | Special culture medium for chrysanthemum chanetii |
| CN104396740A (en) * | 2014-10-24 | 2015-03-11 | 柳州市天姿园艺有限公司 | Chrysanthemum nankingense special-purpose medium |
| CN104396739A (en) * | 2014-10-24 | 2015-03-11 | 柳州市天姿园艺有限公司 | Dendranthema vestitum special culture medium |
| CN104285811B (en) * | 2014-10-24 | 2016-01-13 | 柳州市天姿园艺有限公司 | Camomile special culture media |
| CN104285812B (en) * | 2014-10-24 | 2016-01-20 | 柳州市天姿园艺有限公司 | Sneezeweed special culture media |
| CN104304022B (en) * | 2014-10-24 | 2016-06-15 | 柳州市天姿园艺有限公司 | Chrysanthemum special culture media after fertilizer |
| CN104304024B (en) * | 2014-10-24 | 2016-06-15 | 柳州市天姿园艺有限公司 | Pale reddish brown Herba Dendranthematis indici special culture media |
| CN104285808B (en) * | 2014-10-24 | 2016-06-22 | 柳州市天姿园艺有限公司 | Cut Flower Chrysanthemum Morifolium special culture media |
| CN104351049B (en) * | 2014-10-24 | 2016-08-17 | 柳州市天姿园艺有限公司 | Cineraria special culture media |
| CN104351050B (en) * | 2014-10-24 | 2016-08-24 | 柳州市天姿园艺有限公司 | Little red chrysanthemum special culture media |
| CN104396739B (en) * | 2014-10-24 | 2016-09-07 | 柳州市天姿园艺有限公司 | Mao Huaju special culture media |
| CN107455391A (en) * | 2017-08-15 | 2017-12-12 | 河南省农业科学院园艺研究所 | A kind of grafting activator and the method for improving graft survival rate |
| CN109329052A (en) * | 2018-10-09 | 2019-02-15 | 中国中医科学院中药研究所 | Aristolochia contorta tissue-cultured seedling expanding propagation method |
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Open date: 20081022 |