CN104285812A - Special culture medium for sneezeweed - Google Patents
Special culture medium for sneezeweed Download PDFInfo
- Publication number
- CN104285812A CN104285812A CN201410574227.7A CN201410574227A CN104285812A CN 104285812 A CN104285812 A CN 104285812A CN 201410574227 A CN201410574227 A CN 201410574227A CN 104285812 A CN104285812 A CN 104285812A
- Authority
- CN
- China
- Prior art keywords
- parts
- sneezeweed
- culture medium
- special culture
- acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000006900 Helenium autumnale Nutrition 0.000 title claims abstract description 16
- 235000004833 smallhead sneezeweed Nutrition 0.000 title claims abstract description 16
- 235000006891 sneezeweed Nutrition 0.000 title claims abstract description 16
- 239000001963 growth medium Substances 0.000 title claims abstract description 15
- 241000610694 Hymenoxys hoopesii Species 0.000 title 1
- 244000013628 Achillea ptarmica Species 0.000 claims abstract description 16
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims abstract description 8
- RZCJYMOBWVJQGV-UHFFFAOYSA-N 2-naphthyloxyacetic acid Chemical compound C1=CC=CC2=CC(OCC(=O)O)=CC=C21 RZCJYMOBWVJQGV-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229920001817 Agar Polymers 0.000 claims abstract description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 7
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 claims abstract description 7
- 240000008042 Zea mays Species 0.000 claims abstract description 7
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 7
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 7
- 239000008272 agar Substances 0.000 claims abstract description 7
- 235000020197 coconut milk Nutrition 0.000 claims abstract description 7
- 235000005822 corn Nutrition 0.000 claims abstract description 7
- 239000008103 glucose Substances 0.000 claims abstract description 7
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 claims abstract description 7
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims abstract description 7
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229960001669 kinetin Drugs 0.000 claims abstract description 7
- 239000000843 powder Substances 0.000 claims abstract description 7
- 235000015193 tomato juice Nutrition 0.000 claims abstract description 7
- UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 claims abstract description 7
- UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229940023877 zeatin Drugs 0.000 claims abstract description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 5
- 229930006000 Sucrose Natural products 0.000 claims description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 6
- 239000000413 hydrolysate Substances 0.000 claims description 6
- 230000001939 inductive effect Effects 0.000 claims description 6
- 229910000358 iron sulfate Inorganic materials 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- YGGXZTQSGNFKPJ-UHFFFAOYSA-N methyl 2-naphthalen-1-ylacetate Chemical compound C1=CC=C2C(CC(=O)OC)=CC=CC2=C1 YGGXZTQSGNFKPJ-UHFFFAOYSA-N 0.000 claims description 6
- 239000006870 ms-medium Substances 0.000 claims description 6
- 239000005720 sucrose Substances 0.000 claims description 6
- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 claims description 6
- 229910052799 carbon Inorganic materials 0.000 claims description 3
- OKTJSMMVPCPJKN-YPZZEJLDSA-N carbon-10 atom Chemical group [10C] OKTJSMMVPCPJKN-YPZZEJLDSA-N 0.000 claims description 3
- 230000004083 survival effect Effects 0.000 abstract description 2
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 abstract 1
- LXAHHHIGZXPRKQ-UHFFFAOYSA-N 5-fluoro-2-methylpyridine Chemical compound CC1=CC=C(F)C=N1 LXAHHHIGZXPRKQ-UHFFFAOYSA-N 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 abstract 1
- 108010009736 Protein Hydrolysates Proteins 0.000 abstract 1
- 240000000111 Saccharum officinarum Species 0.000 abstract 1
- 235000007201 Saccharum officinarum Nutrition 0.000 abstract 1
- 235000003891 ferrous sulphate Nutrition 0.000 abstract 1
- 239000011790 ferrous sulphate Substances 0.000 abstract 1
- 239000003617 indole-3-acetic acid Substances 0.000 abstract 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 abstract 1
- 235000016709 nutrition Nutrition 0.000 abstract 1
- 230000035764 nutrition Effects 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 241000131317 Capitulum Species 0.000 description 1
- 235000005979 Citrus limon Nutrition 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a special culture medium for sneezeweed. The special culture medium is prepared from the following substances in parts by weight: 70-80 parts of an MS culture medium, 15-20 parts of ferrous sulfate, 8-10 parts of EDTA disodium, 6-8 parts of sugarcane, 6-8 parts of glucose, 8-10 parts of lactoalbumin hydrolysate, 8-12 parts of corn endosperm, 6-12 parts of tomato juice, 3-12 parts of coconut milk, 3-5 parts of indoleacetic acid, 3-6 parts of indolebutyric acid, 2-5 parts of naphthylacetic acid, 3-6 parts of naphthoxyacetic acid, 1-5 parts of ABT rooting powder, 1-4 parts of zeatin, 1-4 parts of kinetin, 20-30 parts of agar, 5-15 parts of activated carbon and 40-50 parts of water. The special culture medium for the sneezeweed is prepared according to the plant characteristics of the sneezeweed, is rich in nutrition, is capable of effectively improving the survival rate of the sneezeweed and has excellent market prospect.
Description
Technical field
The present invention relates to sneezeweed special culture media.
Background technology
Sneezeweed, the wealthy lanceolar of leaf, capitulum is born in stem top, ligulate flower lemon yellow, and petal is wealthy, and tip is incised, tubular flower yellow green.7 to October of florescence, fructescence September.It requires higher to growing environment, cultivates and survives difficulty, be difficult to meeting the market requirement.
Summary of the invention
Object of the present invention is exactly for above-mentioned defect of the prior art, provides sneezeweed special culture media.
To achieve these goals, technical scheme provided by the invention is: sneezeweed special culture media, prepared by the following material according to parts by weight: MS medium 70-80 part, iron sulfate 15-20 part, EDETATE SODIUM 8-10 part, sucrose 6-8 part, glucose 6-8 part, lactoalbumin hydrolysate 8-10 part, corn embryosperm 8-12 part, Tomato juice 6-12 part, coconut milk 3-12 part, heteroauxin 3-5 part, indolebutyric acid 3-6 part, methyl α-naphthyl acetate 2-5 part, naphthoxyacetic acid 3-6 part, ABT root-inducing powder 1-5 part, zeatin 1-4 part, kinetin 1-4 part, agar 20-30 part, active carbon 5-15 part, water 40-50 part.
Further, above-mentioned sneezeweed special culture media, is prepared by the following material according to parts by weight: MS medium 75 parts, 18 parts, iron sulfate, EDETATE SODIUM 9 parts, sucrose 7 parts, glucose 7 parts, lactoalbumin hydrolysate 9 parts, corn embryosperm 10 parts, Tomato juice 9 parts, coconut milk 8 parts, heteroauxin 4 parts, indolebutyric acid 5 parts, methyl α-naphthyl acetate 4 parts, naphthoxyacetic acid 5 parts, ABT root-inducing powder 3 parts, zeatin 2 parts, kinetin 2 parts, 25 parts, agar, active carbon 10 parts, 45 parts, water.
Beneficial effect of the present invention is: sneezeweed special culture media provided by the invention, nutritious, for the preparation of sneezeweed plant characteristic, can effectively improve its survival rate, good market prospects.
Embodiment
embodiment 1:
Sneezeweed special culture media, is prepared by the following material according to parts by weight: MS medium 70-80 part, iron sulfate 15-20 part, EDETATE SODIUM 8-10 part, sucrose 6-8 part, glucose 6-8 part, lactoalbumin hydrolysate 8-10 part, corn embryosperm 8-12 part, Tomato juice 6-12 part, coconut milk 3-12 part, heteroauxin 3-5 part, indolebutyric acid 3-6 part, methyl α-naphthyl acetate 2-5 part, naphthoxyacetic acid 3-6 part, ABT root-inducing powder 1-5 part, zeatin 1-4 part, kinetin 1-4 part, agar 20-30 part, active carbon 5-15 part, water 40-50 part.
embodiment 2:
Sneezeweed special culture media, is prepared by the following material according to parts by weight: MS medium 75 parts, 18 parts, iron sulfate, EDETATE SODIUM 9 parts, sucrose 7 parts, glucose 7 parts, lactoalbumin hydrolysate 9 parts, corn embryosperm 10 parts, Tomato juice 9 parts, coconut milk 8 parts, heteroauxin 4 parts, indolebutyric acid 5 parts, methyl α-naphthyl acetate 4 parts, naphthoxyacetic acid 5 parts, ABT root-inducing powder 3 parts, zeatin 2 parts, kinetin 2 parts, 25 parts, agar, active carbon 10 parts, 45 parts, water.
Last it is noted that the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment to invention has been detailed description, for a person skilled in the art, it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (2)
1. sneezeweed special culture media, it is characterized in that, prepared by the following material according to parts by weight: MS medium 70-80 part, iron sulfate 15-20 part, EDETATE SODIUM 8-10 part, sucrose 6-8 part, glucose 6-8 part, lactoalbumin hydrolysate 8-10 part, corn embryosperm 8-12 part, Tomato juice 6-12 part, coconut milk 3-12 part, heteroauxin 3-5 part, indolebutyric acid 3-6 part, methyl α-naphthyl acetate 2-5 part, naphthoxyacetic acid 3-6 part, ABT root-inducing powder 1-5 part, zeatin 1-4 part, kinetin 1-4 part, agar 20-30 part, active carbon 5-15 part, water 40-50 part.
2. sneezeweed special culture media according to claim 1, it is characterized in that, prepared by the following material according to parts by weight: MS medium 75 parts, 18 parts, iron sulfate, EDETATE SODIUM 9 parts, sucrose 7 parts, glucose 7 parts, lactoalbumin hydrolysate 9 parts, corn embryosperm 10 parts, Tomato juice 9 parts, coconut milk 8 parts, heteroauxin 4 parts, indolebutyric acid 5 parts, methyl α-naphthyl acetate 4 parts, naphthoxyacetic acid 5 parts, ABT root-inducing powder 3 parts, zeatin 2 parts, kinetin 2 parts, 25 parts, agar, active carbon 10 parts, 45 parts, water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410574227.7A CN104285812B (en) | 2014-10-24 | 2014-10-24 | Sneezeweed special culture media |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410574227.7A CN104285812B (en) | 2014-10-24 | 2014-10-24 | Sneezeweed special culture media |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104285812A true CN104285812A (en) | 2015-01-21 |
| CN104285812B CN104285812B (en) | 2016-01-20 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410574227.7A Expired - Fee Related CN104285812B (en) | 2014-10-24 | 2014-10-24 | Sneezeweed special culture media |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104285812B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1915001A (en) * | 2006-08-31 | 2007-02-21 | 中国药科大学 | Technique for breeding pyrethrum of white flower |
| CN101288381A (en) * | 2007-04-18 | 2008-10-22 | 天津市园林绿化研究所 | Collective medium for flower tissue culture |
| CN102301952A (en) * | 2011-07-19 | 2012-01-04 | 北京林业大学 | Method for breeding chamomile |
| CN102939901A (en) * | 2012-11-13 | 2013-02-27 | 巴中市光雾山植物研究所 | Chrysanthemum one-step tissue culture quick breeding method |
-
2014
- 2014-10-24 CN CN201410574227.7A patent/CN104285812B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1915001A (en) * | 2006-08-31 | 2007-02-21 | 中国药科大学 | Technique for breeding pyrethrum of white flower |
| CN101288381A (en) * | 2007-04-18 | 2008-10-22 | 天津市园林绿化研究所 | Collective medium for flower tissue culture |
| CN102301952A (en) * | 2011-07-19 | 2012-01-04 | 北京林业大学 | Method for breeding chamomile |
| CN102939901A (en) * | 2012-11-13 | 2013-02-27 | 巴中市光雾山植物研究所 | Chrysanthemum one-step tissue culture quick breeding method |
Non-Patent Citations (2)
| Title |
|---|
| 彭玉华等: "野生植物资源甘菊的研究进展", 《中国园艺文摘》 * |
| 杨英军等: "《园艺植物生物技术与原理与方法》", 31 December 2007, 农业出版社 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104285812B (en) | 2016-01-20 |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20170925 Address after: Calculation of business R & D center eleven layer research room 530029 Nanning city the Guangxi Zhuang Autonomous Region Qingxiu District Bauhinia Road No. 1 Nanning international internet dedicated channel and cloud Patentee after: GUANGXI YIMU AGRICULTURAL TECHNOLOGY Co.,Ltd. Address before: 545002, the Guangxi Zhuang Autonomous Region, Liuzhou North Long Pond flower demonstration Office Patentee before: LIUZHOU TIANZI HORTICULTURE Co.,Ltd. |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160120 |