CN103250639B - A kind of method of seed culture of dendrobium candidum - Google Patents
A kind of method of seed culture of dendrobium candidum Download PDFInfo
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- CN103250639B CN103250639B CN201310139250.9A CN201310139250A CN103250639B CN 103250639 B CN103250639 B CN 103250639B CN 201310139250 A CN201310139250 A CN 201310139250A CN 103250639 B CN103250639 B CN 103250639B
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- protocorm
- dendrobium candidum
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- 241000026010 Dendrobium candidum Species 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title claims abstract description 16
- 238000011218 seed culture Methods 0.000 title claims abstract description 11
- 230000004069 differentiation Effects 0.000 claims abstract description 7
- 229920001817 Agar Polymers 0.000 claims description 12
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 12
- 229930006000 Sucrose Natural products 0.000 claims description 12
- 239000008272 agar Substances 0.000 claims description 12
- 239000000843 powder Substances 0.000 claims description 12
- 230000001954 sterilising effect Effects 0.000 claims description 12
- 238000004659 sterilization and disinfection Methods 0.000 claims description 12
- 239000005720 sucrose Substances 0.000 claims description 12
- SQGYWYIDSFRISU-UHFFFAOYSA-N 6-benzyl-7h-purin-2-amine Chemical class C=12NC=NC2=NC(N)=NC=1CC1=CC=CC=C1 SQGYWYIDSFRISU-UHFFFAOYSA-N 0.000 claims description 9
- 235000013399 edible fruits Nutrition 0.000 claims description 6
- 230000001939 inductive effect Effects 0.000 claims description 6
- -1 methyl α-naphthyl acetates Chemical class 0.000 claims description 6
- 241000202807 Glycyrrhiza Species 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 206010011224 Cough Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention relates to a kind of method of seed culture of dendrobium candidum, the method comprises: step 1): protocorm is induced; Step 2): Protocorm Multiplication; Step 3): protocorm differentiation; And step 4): take root and strong sprout.The method of the seed culture of dendrobium candidum of the present invention is that the large-scale industrialized production of dendrobium candidum provides technical basis.
Description
Technical field
The present invention relates to agriculture field, specifically, the present invention relates to a kind of method of seed culture of dendrobium candidum.
Background technology
Dendrobium candidum is the one tradition rare Chinese medicine of China, the effect such as have nourishing Yin and clearing heat, the beneficial stomach that promotes the production of body fluid, moisten the lung and relieve the cough, medical value and economic worth high.In recent years, owing to manually excessively excavating the destruction with natural habitat, wild stone solution resource is day by day exhausted, and market supply is in short supply.The seed of dendrobium candidum is very tiny, be difficult to sprout, and traditional division propagation method speed is slow, is difficult to meet the need of market under natural conditions.Therefore, the method for the seed culture finding a kind of dendrobium candidum is needed.
Summary of the invention
The object of the present invention is to provide a kind of method of seed culture of dendrobium candidum.
In order to realize object of the present invention, the invention provides a kind of method of seed culture of dendrobium candidum, the method comprises:
Step 1): protocorm is induced;
Step 2): Protocorm Multiplication;
Step 3): protocorm differentiation; And
Step 4): take root and strong sprout.
Preferably, in described step 1) in, 30 grams of sucrose, 10 grams of agar powders, 12 grams of Radix Glycyrrhizaes and 10 grams of roots of gansui are placed in 1 premium on currency, and adjust ph is 5.4, and at 120 DEG C, heat sterilization 15 minutes, obtains medium; The uncracked mellow fruit pod of dendrobium candidum is cut fruit pod, takes out seed and be inoculated in above-mentioned medium, illumination cultivation 20 days.
Preferably, in described step 2) in, 30 grams of sucrose, 10 grams of agar powders, 5 grams of 6-benzyl aminopurines are placed in 1 premium on currency, and adjust ph is 5.4, and at 120 DEG C, heat sterilization 15 minutes, obtains medium; Choose the protocorm that diameter is 1.0mm, be inoculated in above-mentioned medium and carry out propagation 20 days.
Preferably, in described step 3) in, 30 grams of sucrose, 10 grams of agar powders, 5 grams of 6-benzyl aminopurines, 5 grams of methyl α-naphthyl acetates are placed in 1 premium on currency, and adjust ph is 5.4, and at 120 DEG C, heat sterilization 15 minutes, obtains medium; Protocorm group after propagation is inoculated on above-mentioned medium and carries out induced bundle bud 20 days.
Preferably, in described step 4) in, 30 grams of sucrose, 10 grams of agar powders, 5 grams of 6-benzyl aminopurines, 5 grams of methyl α-naphthyl acetates are placed in 1 premium on currency, and adjust ph is 5.4, and at 120 DEG C, heat sterilization 15 minutes, obtains medium; By differentiation-inducing clump bud cutting and separating simple bud, to be inoculated on above-mentioned medium inducing adventitious root 20 days.
The method of the seed culture of dendrobium candidum of the present invention is that the large-scale industrialized production of dendrobium candidum provides technical basis.
Embodiment
Below by way of the description of embodiment, the invention will be further described, but this is not limitation of the present invention, those skilled in the art are according to basic thought of the present invention, various amendment or improvement can be made, but only otherwise depart from basic thought of the present invention, all within the scope of the present invention.
Embodiment 1
A method for the seed culture of dendrobium candidum, the method comprises:
Step 1): protocorm is induced;
Step 2): Protocorm Multiplication;
Step 3): protocorm differentiation; And
Step 4): take root and strong sprout.
In described step 1) in, 30 grams of sucrose, 10 grams of agar powders, 12 grams of Radix Glycyrrhizaes and 10 grams of roots of gansui are placed in 1 premium on currency, and adjust ph is 5.4, and at 120 DEG C, heat sterilization 15 minutes, obtains medium; The uncracked mellow fruit pod of dendrobium candidum is cut fruit pod, takes out seed and be inoculated in above-mentioned medium, illumination cultivation 20 days.
In described step 2) in, 30 grams of sucrose, 10 grams of agar powders, 5 grams of 6-benzyl aminopurines are placed in 1 premium on currency, and adjust ph is 5.4, and at 120 DEG C, heat sterilization 15 minutes, obtains medium; Choose the protocorm that diameter is 1.0mm, be inoculated in above-mentioned medium and carry out propagation 20 days.
In described step 3) in, 30 grams of sucrose, 10 grams of agar powders, 5 grams of 6-benzyl aminopurines, 5 grams of methyl α-naphthyl acetates are placed in 1 premium on currency, and adjust ph is 5.4, and at 120 DEG C, heat sterilization 15 minutes, obtains medium; Protocorm group after propagation is inoculated on above-mentioned medium and carries out induced bundle bud 20 days.
In described step 4) in, 30 grams of sucrose, 10 grams of agar powders, 5 grams of 6-benzyl aminopurines, 5 grams of methyl α-naphthyl acetates are placed in 1 premium on currency, and adjust ph is 5.4, and at 120 DEG C, heat sterilization 15 minutes, obtains medium; By differentiation-inducing clump bud cutting and separating simple bud, to be inoculated on above-mentioned medium inducing adventitious root 20 days, rooting rate reaches 100%, and average every strain has adventive root 4.5, the long 2.5cm of average root, and the average plant height 5.8cm of seedling, Plant Leaf look dark green, robust growth.
Claims (1)
1. a method for the seed culture of dendrobium candidum, the method comprises:
Step 1): protocorm is induced;
Step 2): Protocorm Multiplication;
Step 3): protocorm differentiation; And
Step 4): take root and strong sprout;
In described step 1) in, 30 grams of sucrose, 10 grams of agar powders, 12 grams of Radix Glycyrrhizaes and 10 grams of roots of gansui are placed in 1 premium on currency, and adjust ph is 5.4, and at 120 DEG C, heat sterilization 15 minutes, obtains medium; The uncracked mellow fruit pod of dendrobium candidum is cut fruit pod, takes out seed and be inoculated in above-mentioned medium, illumination cultivation 20 days;
In described step 2) in, 30 grams of sucrose, 10 grams of agar powders, 5 grams of 6-benzyl aminopurines are placed in 1 premium on currency, and adjust ph is 5.4, and at 120 DEG C, heat sterilization 15 minutes, obtains medium; Choose the protocorm that diameter is 1.0mm, be inoculated in above-mentioned medium and carry out propagation 20 days;
In described step 3) in, 30 grams of sucrose, 10 grams of agar powders, 5 grams of 6-benzyl aminopurines, 5 grams of methyl α-naphthyl acetates are placed in 1 premium on currency, and adjust ph is 5.4, and at 120 DEG C, heat sterilization 15 minutes, obtains medium; Protocorm group after propagation is inoculated on above-mentioned medium and carries out induced bundle bud 20 days;
In described step 4) in, 30 grams of sucrose, 10 grams of agar powders, 5 grams of 6-benzyl aminopurines, 5 grams of methyl α-naphthyl acetates are placed in 1 premium on currency, and adjust ph is 5.4, and at 120 DEG C, heat sterilization 15 minutes, obtains medium; By differentiation-inducing clump bud cutting and separating simple bud, to be inoculated on above-mentioned medium inducing adventitious root 20 days.
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| CN201310139250.9A CN103250639B (en) | 2013-04-19 | 2013-04-19 | A kind of method of seed culture of dendrobium candidum |
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| CN201310139250.9A CN103250639B (en) | 2013-04-19 | 2013-04-19 | A kind of method of seed culture of dendrobium candidum |
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| CN103250639A CN103250639A (en) | 2013-08-21 |
| CN103250639B true CN103250639B (en) | 2015-08-12 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104488705A (en) * | 2014-12-03 | 2015-04-08 | 柳州市泓吉农业科技有限公司 | Method for cultivating tissue culture seedlings of dendrobium nobile lindl |
| CN104542286A (en) * | 2014-12-31 | 2015-04-29 | 贵州润芝霖生态农业科技开发有限公司 | Production method for dendrobium officinale tissue culture seedlings |
| CN107926703A (en) * | 2017-12-04 | 2018-04-20 | 浙江东来天然生物制品有限公司 | A kind of high selenium-enriched officinal dendrobium stem seeding cultivating method of transplanting survival rate |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101564008A (en) * | 2008-04-24 | 2009-10-28 | 中国医学科学院药用植物研究所 | Hormone-free cultivation and rapid propagation method of dendrobium candidum axenic seedlings |
| CN101933456A (en) * | 2009-06-29 | 2011-01-05 | 西双版纳增靓生物科技有限公司 | Method for quickly breeding seedlings of dendrobium officinale capsule |
| CN102422811A (en) * | 2011-10-06 | 2012-04-25 | 红河州巨丰生物科技有限公司 | Dendrobium officinale tissue culture reproduction method |
| CN102948368A (en) * | 2012-11-13 | 2013-03-06 | 安徽新津铁皮石斛开发有限公司 | Tissue culture method of dendrobium candidum |
| CN102960245A (en) * | 2012-11-16 | 2013-03-13 | 江阴振江生物科技有限公司 | Artificial breeding and cultivating method of Dendrobium officinale |
-
2013
- 2013-04-19 CN CN201310139250.9A patent/CN103250639B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101564008A (en) * | 2008-04-24 | 2009-10-28 | 中国医学科学院药用植物研究所 | Hormone-free cultivation and rapid propagation method of dendrobium candidum axenic seedlings |
| CN101933456A (en) * | 2009-06-29 | 2011-01-05 | 西双版纳增靓生物科技有限公司 | Method for quickly breeding seedlings of dendrobium officinale capsule |
| CN102422811A (en) * | 2011-10-06 | 2012-04-25 | 红河州巨丰生物科技有限公司 | Dendrobium officinale tissue culture reproduction method |
| CN102948368A (en) * | 2012-11-13 | 2013-03-06 | 安徽新津铁皮石斛开发有限公司 | Tissue culture method of dendrobium candidum |
| CN102960245A (en) * | 2012-11-16 | 2013-03-13 | 江阴振江生物科技有限公司 | Artificial breeding and cultivating method of Dendrobium officinale |
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Effective date of registration: 20211223 Address after: 315400 No. 303 Ruhu East Road, Simen Town, Yuyao City, Ningbo City, Zhejiang Province Patentee after: Zhejiang jianjiuhe Pharmaceutical Group Co.,Ltd. Address before: 315434 No. 1, xiangquanwan Road, Shangzhuang village, luting Township, Yuyao City, Ningbo City, Zhejiang Province Patentee before: ZHEJIANG YUNZHITANG BIOTECHNOLOGY Co.,Ltd. |
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Denomination of invention: A Method for Seed Culture of Dendrobium officinale Granted publication date: 20150812 Pledgee: Ningbo Yuyao Rural Cooperative Bank Pledgor: Zhejiang jianjiuhe Pharmaceutical Group Co.,Ltd. Registration number: Y2024980015022 |