CN102960245A - Artificial breeding and cultivating method of Dendrobium officinale - Google Patents
Artificial breeding and cultivating method of Dendrobium officinale Download PDFInfo
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- 238000009395 breeding Methods 0.000 title abstract 6
- 230000001488 breeding effect Effects 0.000 title abstract 6
- 241000238631 Hexapoda Species 0.000 claims abstract description 13
- 239000002609 medium Substances 0.000 claims description 40
- 241000026010 Dendrobium candidum Species 0.000 claims description 39
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 claims description 24
- 229920001817 Agar Polymers 0.000 claims description 24
- 239000008272 agar Substances 0.000 claims description 24
- 244000061456 Solanum tuberosum Species 0.000 claims description 18
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Abstract
The invention discloses an artificial breeding and cultivating method of Dendrobium officinale. The artificial breeding and cultivating method of the Dendrobium officinale comprises nine steps of disinfecting seeds, culturing tissues, domesticating, transplanting and the like. The artificial breeding and cultivating method adopts a tissue culturing method to grow seedlings and components and culturing environments of a culture medium are adjusted in time along the growth phase of the Dendrobium officinale, so that the problems that seeds of the Dendrobium officinale are difficult to germinate and a cultivation variety is degenerated are solved; the artificial breeding and cultivating method adopts suitable domesticating conditions and steps to guarantee that the cultivated seedlings are transplanted after adapting to the natural environment; the cultivated seedlings which are domesticated are cultivated by a two-section method so as to be good for improving the quality of the Dendrobium officinale and the survival rate of the cultivated seedlings; and the method can ensure that the seedling cultivating and breeding speed is high, improves the seedling cultivating, domesticating and transplanting survival rates and can avoid phenomenon of generating weeds and injurious insects in a common process of large-field cultivation.
Description
Technical field
The present invention relates to field of plant growing technology, be specifically related to a kind of dendrobium candidum artificial culture cultivation method.
Background technology
Dendrobium candidum is a kind of extremely valuable ingredients of traditional Chinese medicine, because it has the immunologic function of enhancing, antitumor, the anti-ageing unique effects of waiting for a long time, therefore at home in tcm clinical practice prescription and the Chinese patent drug raw material of industry for export amount constantly increase, demand domestic, the international market is also growing, price soars all the way, and makes dendrobium candidum increasingly seem rare famous and precious.Wild Dendrodium output is rare, and its self-sow is slow in addition, needs that far away can not satisfying the market.Dendrobium candidum is the orchid family Dendrobium Sw, and seed is minimum, without endosperm, need under the natural conditions could sprout with some mycosymbiosis, in the natural situation germination rate only ten thousand/, and there is degradation phenomena in cultivar.
In recent years, some scientific research institutions of China and business unit have carried out a large amount of explorations and test to the training of the group of dendrobium candidum and artificial cultivation technique, have obtained some achievements, also so that in recent years artificial cultivation dendrobium candidum output increase to some extent.But the artificial culture of dendrobium candidum exists still that seeding propagation speed is slow, training tissue culture seedling and transplanting survival rate is low, artificial planting product active constituent content is low problem, has seriously restricted industrialization, the large-scale development of dendrobium candidum.In addition, the general field planting of dendrobium candidum can be followed growth of weeds, and is vulnerable to the insect pests such as snail.
Summary of the invention
The object of the invention is to overcome the defective that exists in the prior art, design a kind of dendrobium candidum artificial culture cultivation method, it is higher to guarantee that group is cultivated seedling and propagating speed, improve training tissue culture seedling and transplanting survival rate, and weeds and the insect phenomenon that can avoid general field production to produce, make the growing environment of dendrobium candidum more near natural environment, thereby guarantee the quality of dendrobium candidum.
For achieving the above object, technical scheme of the present invention provides a kind of dendrobium candidum artificial culture cultivation method, it is characterized in that, described dendrobium candidum artificial culture cultivation method may further comprise the steps:
S1: Seeds of Dendrobium Candidum is carried out the routine disinfection sterilization;
S2: the Seeds of Dendrobium Candidum after will sterilizing is put in the blake bottle that germination medium is housed and is sprouted, under the environment of 24 ℃～26 ℃ of temperature, humidity 65%～75%, illumination 2000～2500lx, cultivate and formed protocorm in 3 months, contain 1/2MS minimal medium, 0.2 mg/L α-naphthaleneacetic acid, 100g/ L Coconut Juice and 4g/ L agar in the described germination medium, described α-naphthaleneacetic acid, Coconut Juice, agar content are with the stereometer of 1/2MS minimal medium;
S3: the protocorm of gained among the S2 changed over to induced bundle is housed sprouts to cultivate in the blake bottle of medium and formed seedling in 3 months, described induced bundle is sprouted and is contained murphy juice and the 4g/ L agar that 1/2MS minimal medium, 0.3mg/L α-naphthaleneacetic acid, 80g/ L potato press in the medium, and described α-naphthaleneacetic acid, potato, agar content are with the stereometer of 1/2MS minimal medium;
S4: change the seedling of gained among the S3 in the blake bottle that the seedling medium is housed cultivation formation in 3 months seedling, contain murphy juice and 4g/ L agar that 1/2MS minimal medium, 0.5mg/L α-naphthaleneacetic acid, 80g/ L potato press in the described seedling medium, described α-naphthaleneacetic acid, potato, agar content are with the stereometer of 1/2MS minimal medium;
S5: change the seedling of gained among the S4 in the blake bottle that the strengthening seedling and rooting medium is housed cultivation formation in 4 months seedlings, contain murphy juice and 4g/ L agar that 1/2MS minimal medium, 0.4mg/L α-naphthaleneacetic acid, 60g/ L potato press in the described strengthening seedling and rooting medium, described α-naphthaleneacetic acid, potato, agar content are with the stereometer of 1/2MS minimal medium;
S6: the airtight blake bottle that gained among the S5 is included seedlings placed on the seedbed in the glass booth 6～8 days, then opened blake bottle bottle cap hardening 2～3 days;
S7: the cultivation seedling after the hardening among the S6 is taken out from blake bottle, clean root, dry and wait to transplant;
S8: the S7 gained is cultivated seedling migrate to and cultivated in the basin of cave 10～13 months, be filled with cultivation matrix in the basin of described cave, the root of described cultivation seedling is planted fully in described cultivation matrix, described cave basin is arranged on the interior implant bed of glass temperature control green house, liftoff 70～the 80cm of described implant bed, temperature in the described glass temperature control green house is 20 ℃～28 ℃, and humidity is 75%～85%;
S9: with the dendrobium candidum transplantation of seedlings of gained among the S8 in the nonwoven planting bag that is filled with cultivation matrix, described nonwoven planting bag is arranged on the seedbed in the monomer booth, be provided with metalling between described seedbed and the nonwoven planting bag bottom, canopy temperature is 10 ℃～35 ℃, and humidity is 75%～85%.
Wherein, the cultivation matrix among described S8 and the S9 forms and comprises sawmilling and bark, and the weight accounting of described bark is 40%～55%.
Wherein, basin top in cave is provided with the insect protected plate among the described S8, and the distance between described insect protected plate and the basin top, described cave is 25～35cm.
Wherein, basin top in cave is provided with equipment of sprinkler irrigation among the described S8, and the distance between the shower nozzle of described equipment of sprinkler irrigation and the basin top, described cave is 140～160cm.
Wherein, in the seedbed area, the density that the nonwoven planting bag is put among the described S9 is 28000～33000 bags/mu.
Wherein, the thickness of described metalling is 6～8cm, can effectively completely cut off in the soil insect such as weeds and snail to the infringement of dendrobium candidum plant.
Advantage of the present invention and beneficial effect are: the present invention adopts the method for tissue culture seedling, and in time adjusts nutrient media components and culture environment with the vegetative stage of dendrobium candidum, solves Seeds of Dendrobium Candidum and sprouts problem difficult and that cultivar degenerates; Adopt suitable hardening condition and step, guarantee to allow the cultivation seedling accept to transplant again behind the reform of nature environment; Cultivation seedling after the hardening adopts the two-stage method cultivation, is conducive to improve the quality of dendrobium candidum and the survival rate of cultivating seedling; It is higher that the method can guarantee that group is cultivated seedling and propagating speed, improves training tissue culture seedling and transplanting survival rate, and weeds and the insect infestation that can avoid general field production to produce.
Embodiment
Below in conjunction with embodiment, the specific embodiment of the present invention is further described.Following examples only are used for technical scheme of the present invention more clearly is described, and can not limit protection scope of the present invention with this.
Embodiment 1
Embodiment is a kind of dendrobium candidum artificial culture cultivation method, and the method may further comprise the steps:
S1: seed disinfection is sterilized;
S2: put in the germination medium of 1/2MS minimal medium+0.2 mg/L α-naphthaleneacetic acid+100g/ L Coconut Juice+4g/ L agar and sprout, α-naphthaleneacetic acid, Coconut Juice, agar content are with the stereometer of 1/2MS minimal medium, 24 ℃～26 ℃ of temperature, humidity 65%～75% was cultivated 3 months under the environment of illumination 2200～2400lx;
S3: the induced bundle that changes the protocorm that forms after 3 months over to the murphy juice that 1/2MS minimal medium+0.3mg/L α-naphthaleneacetic acid+80g/ L potato presses+4g/ L agar is sprouted and is carried out for the second time 3 months by a definite date cultivation in the medium, form after 3 months 1cm less than seedling, α-naphthaleneacetic acid, potato, agar content are with the stereometer of 1/2MS minimal medium;
S4: seedling is changed in the seedling medium of the murphy juice that 1/2MS minimal medium+0.5mg/L α-naphthaleneacetic acid+80g/ L potato presses+4g/ L agar and carry out for the third time 3 months by a definite date cultivation, seedling growing height after 3 months is 3cm～4cm, and α-naphthaleneacetic acid, potato, agar content are with the stereometer of 1/2MS minimal medium;
S5: seedling is put in the strengthening seedling and rooting medium of the murphy juice that 1/2MS minimal medium+0.4mg/L α-naphthaleneacetic acid+60g/ L potato presses+4g/ L agar, carry out the 4th time 4 months by a definite date cultivation, seedlings height after 4 months is about 6cm～7cm, and α-naphthaleneacetic acid, potato, agar content are with the stereometer of 1/2MS minimal medium;
S6: blake bottle is positioned on the seedbed in the glass booth, and 7 days by a definite date, the leaf of seedling to be cultivated began to deepen green, and uncork lid hardening is 2 days again, produced the problems such as poor growth, survival rate are not high thereby hardening can make dendrobium candidum seedling reform of nature environment solve;
S7: at last seedling is taken out, clean the medium of root, will cultivate at last seedling and dry and wait to transplant;
S8: in order to improve the transplanting survival rate of bottle outlet seedling, cultivate seedling and adopt the two-stage method cultivation.The first paragraph cultivation: dendrobium candidum seedling planting matrix is that mass ratio is sawmilling and the bark of 1:1 allotment, matrix is evenly put into the cave basin, the root of seedling is planted fully in matrix, matrix should not be pressed too consolidation, on the seedbed of cave basin as for liftoff 75cm, guaranteed that like this seedling growth has the drainability of ventilating preferably in the basin of cave.The 30cm place hangs the insect protected plate above the basin of cave, the insect of the most of flight of absorption; Equipment of sprinkler irrigation is placed at the 150cm place above the basin of cave, is conducive to evenly irrigate and regulate the humidity of glass greenhouse interior 78%～82%, keeps 23 ℃～25 ℃ of temperature of shed.Automatically regulate the growth conditions such as humidity, temperature, illumination of glass greenhouse by Internet of Things, the survival rate of dendrobium candidum bottle seedling reaches more than 99%;
S9: second segment cultivation: the large transplantation of seedlings of dendrobium candidum after in the glass temperature control green house, growing a year to the monomer booth.Directly adopt the field production pattern in the monomer booth, spread thick stone in the seedbed with isolation soil, culture dish changes larger nonwoven into by the cave basin and cultivates bag, put into identical cultivation matrix in the cultivation bag, seedlings are transplanted into cultivate bag, sack directly is placed on the metalling, the thickness of metalling is 7cm, the density that seedlings are put is about 31000 bags/mu, keeps humidity 78%～82% in the canopy, 20 ℃～25 ℃ of temperature.This cropping pattern has guaranteed the ventilation drainability of seedlings, the weeds and the insect that have also avoided general field production to produce.Above the seedbed, put equally insect protected plate and equipment of sprinkler irrigation.Utilize sunshade net, skylight side window control temperature and illumination.Dendrobium candidum in the monomer booth, growing environment have guaranteed the quality of dendrobium candidum like this closer to natural environment.
Above-mentioned MS minimal medium is that Murashige and Skoog were the tobacco cell Training Design in 1962, be characterized in that mineral salt and ion concentration are higher, more stable ionic equilibrium solution, its nitrate content is high, quantity and the ratio of its nutrient are suitable, can satisfy nutrition and the physiological requirements of plant cell, thereby the scope of application is wider, the most plants tissue-culturing quick-propagation uses it as the minimal medium of medium.Namely on the concentration of component basis of MS minimal medium, macroelement concentration reduces by half the 1/2MS minimal medium, and microelement concentration is constant.
The above only is preferred embodiment of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (6)
1. a dendrobium candidum artificial culture cultivation method is characterized in that, described dendrobium candidum artificial culture cultivation method may further comprise the steps:
S1: Seeds of Dendrobium Candidum is carried out the routine disinfection sterilization;
S2: the Seeds of Dendrobium Candidum after will sterilizing is put in the blake bottle that germination medium is housed and is sprouted, under the environment of 24 ℃～26 ℃ of temperature, humidity 65%～75%, illumination 2000～2500lx, cultivate and formed protocorm in 3 months, contain 1/2MS minimal medium, 0.2 mg/L α-naphthaleneacetic acid, 100g/ L Coconut Juice and 4g/ L agar in the described germination medium, described α-naphthaleneacetic acid, Coconut Juice, agar content are with the stereometer of 1/2MS minimal medium;
S3: the protocorm of gained among the S2 changed over to induced bundle is housed sprouts to cultivate in the blake bottle of medium and formed seedling in 3 months, described induced bundle is sprouted and is contained murphy juice and the 4g/ L agar that 1/2MS minimal medium, 0.3mg/L α-naphthaleneacetic acid, 80g/ L potato press in the medium, and described α-naphthaleneacetic acid, potato, agar content are with the stereometer of 1/2MS minimal medium;
S4: change the seedling of gained among the S3 in the blake bottle that the seedling medium is housed cultivation formation in 3 months seedling, contain murphy juice and 4g/ L agar that 1/2MS minimal medium, 0.5mg/L α-naphthaleneacetic acid, 80g/ L potato press in the described seedling medium, described α-naphthaleneacetic acid, potato, agar content are with the stereometer of 1/2MS minimal medium;
S5: change the seedling of gained among the S4 in the blake bottle that the strengthening seedling and rooting medium is housed cultivation formation in 4 months seedlings, contain murphy juice and 4g/ L agar that 1/2MS minimal medium, 0.4mg/L α-naphthaleneacetic acid, 60g/ L potato press in the described strengthening seedling and rooting medium, described α-naphthaleneacetic acid, potato, agar content are with the stereometer of 1/2MS minimal medium;
S6: the airtight blake bottle that gained among the S5 is included seedlings placed on the seedbed in the glass booth 6～8 days, then opened blake bottle bottle cap hardening 2～3 days;
S7: the cultivation seedling after the hardening among the S6 is taken out from blake bottle, clean root, dry and wait to transplant;
S8: the S7 gained is cultivated seedling migrate to and cultivated in the basin of cave 10～13 months, be filled with cultivation matrix in the basin of described cave, the root of described cultivation seedling is planted fully in described cultivation matrix, described cave basin is arranged on the interior implant bed of glass temperature control green house, liftoff 70～the 80cm of described implant bed, temperature in the described glass temperature control green house is 20 ℃～28 ℃, and humidity is 75%～85%;
S9: with the dendrobium candidum transplantation of seedlings of gained among the S8 in the nonwoven planting bag that is filled with cultivation matrix, described nonwoven planting bag is arranged on the seedbed in the monomer booth, be provided with metalling between described seedbed and the nonwoven planting bag bottom, canopy temperature is 10 ℃～35 ℃, and humidity is 75%～85%.
2. dendrobium candidum artificial culture cultivation method according to claim 1 is characterized in that, the cultivation matrix among described S8 and the S9 forms and comprises sawmilling and bark, and the weight accounting of described bark is 40%～55%.
3. dendrobium candidum artificial culture cultivation method according to claim 1 is characterized in that, basin top in cave is provided with the insect protected plate among the described S8, and the distance between described insect protected plate and the basin top, described cave is 25～35cm.
4. dendrobium candidum artificial culture cultivation method according to claim 1 is characterized in that, basin top in cave is provided with equipment of sprinkler irrigation among the described S8, and the distance between the shower nozzle of described equipment of sprinkler irrigation and the basin top, described cave is 140～160cm.
5. dendrobium candidum artificial culture cultivation method according to claim 1 is characterized in that, in the seedbed area, the density that the nonwoven planting bag is put among the described S9 is 28000～33000 bags/mu.
6. dendrobium candidum artificial culture cultivation method according to claim 1 is characterized in that, the thickness of described metalling is 6～8cm.
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| CN103202234A (en) * | 2013-04-28 | 2013-07-17 | 陕西师范大学 | Rapid propagation method of dendrobium candidum |
| CN103250639A (en) * | 2013-04-19 | 2013-08-21 | 浙江韵芝堂生物科技有限公司 | Dendrobium officinale seed cultivation method |
| CN103477976A (en) * | 2013-07-12 | 2014-01-01 | 广东省农业科学院作物研究所 | Stem tissue culture seedling method of dendrobium candidum |
| CN105284571A (en) * | 2015-11-23 | 2016-02-03 | 铜仁市金农绿色农业科技有限公司 | Planting method for dendrobium officinale |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6168952B1 (en) * | 1998-08-04 | 2001-01-02 | Korea Kumho Petrochemical Co., Ltd. | Method for producing flowering orchids in vitro |
| CN101564008A (en) * | 2008-04-24 | 2009-10-28 | 中国医学科学院药用植物研究所 | Hormone-free cultivation and rapid propagation method of dendrobium candidum axenic seedlings |
| CN101803515A (en) * | 2010-02-05 | 2010-08-18 | 武汉中大瑞丰生物工程有限公司 | Method for rapidly growing and cultivating dendrobium officinale |
| CN102523881A (en) * | 2012-01-16 | 2012-07-04 | 澄思源生物科技（上海）有限公司 | Industrial cultivation method for dendrobium candidum |
| CN102550271A (en) * | 2012-02-28 | 2012-07-11 | 江苏省中国科学院植物研究所 | Method for commercialized cultivation of container seedling of cold-resistant japanese cinnamon germplasm |
-
2012
- 2012-11-16 CN CN201210460306.6A patent/CN102960245B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6168952B1 (en) * | 1998-08-04 | 2001-01-02 | Korea Kumho Petrochemical Co., Ltd. | Method for producing flowering orchids in vitro |
| CN101564008A (en) * | 2008-04-24 | 2009-10-28 | 中国医学科学院药用植物研究所 | Hormone-free cultivation and rapid propagation method of dendrobium candidum axenic seedlings |
| CN101803515A (en) * | 2010-02-05 | 2010-08-18 | 武汉中大瑞丰生物工程有限公司 | Method for rapidly growing and cultivating dendrobium officinale |
| CN102523881A (en) * | 2012-01-16 | 2012-07-04 | 澄思源生物科技（上海）有限公司 | Industrial cultivation method for dendrobium candidum |
| CN102550271A (en) * | 2012-02-28 | 2012-07-11 | 江苏省中国科学院植物研究所 | Method for commercialized cultivation of container seedling of cold-resistant japanese cinnamon germplasm |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103250639A (en) * | 2013-04-19 | 2013-08-21 | 浙江韵芝堂生物科技有限公司 | Dendrobium officinale seed cultivation method |
| CN103250639B (en) * | 2013-04-19 | 2015-08-12 | 浙江韵芝堂生物科技有限公司 | A kind of method of seed culture of dendrobium candidum |
| CN103202234A (en) * | 2013-04-28 | 2013-07-17 | 陕西师范大学 | Rapid propagation method of dendrobium candidum |
| CN103202234B (en) * | 2013-04-28 | 2014-05-14 | 陕西师范大学 | Rapid propagation method of dendrobium candidum |
| CN103477976A (en) * | 2013-07-12 | 2014-01-01 | 广东省农业科学院作物研究所 | Stem tissue culture seedling method of dendrobium candidum |
| CN103477976B (en) * | 2013-07-12 | 2016-07-20 | 广东省农业科学院作物研究所 | A kind of Herba Dendrobii stem section tissue culture method |
| CN105284571A (en) * | 2015-11-23 | 2016-02-03 | 铜仁市金农绿色农业科技有限公司 | Planting method for dendrobium officinale |
| CN106332757A (en) * | 2016-08-25 | 2017-01-18 | 赵孝巍 | Dendrobe seedling culture technology |
| CN107114223A (en) * | 2017-05-12 | 2017-09-01 | 贵州济生农业科技有限公司 | A kind of cultural method of dendrobium candidum |
| CN107278898A (en) * | 2017-07-24 | 2017-10-24 | 南京仙草堂生物科技有限公司 | A kind of Huangshi dry measure used in former times seedling aseptic seeding rapid propagation method |
| CN107432240A (en) * | 2017-08-01 | 2017-12-05 | 霍山县大化坪秀峰茶业精制厂 | A kind of method for culturing seedlings of Dendrobidium huoshanness |
| CN107212142A (en) * | 2017-08-03 | 2017-09-29 | 南京仙草堂生物科技有限公司 | A kind of preparation method of yellow dendrobe health-care tea |
| CN109452100A (en) * | 2018-12-29 | 2019-03-12 | 神农架神林生物科技有限公司 | The implantation methods of dendrobium candidum |
| CN109644871A (en) * | 2019-01-21 | 2019-04-19 | 花垣县精虹生物科技发展有限公司 | A method suitable for mass production of various orchid medicinal plants |
| CN109644871B (en) * | 2019-01-21 | 2022-01-11 | 花垣县精虹生物科技发展有限公司 | Batch production method suitable for various orchidaceous medicinal plants |
| CN110710455A (en) * | 2019-11-27 | 2020-01-21 | 山东钙康生物工程有限公司 | Tissue culture and rapid propagation method for dendrobium officinale |
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