CN102726294B - In-bottle rooting method for salt tolerance ulmus pumila tissue culture seedling - Google Patents
In-bottle rooting method for salt tolerance ulmus pumila tissue culture seedling Download PDFInfo
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- CN102726294B CN102726294B CN 201210216732 CN201210216732A CN102726294B CN 102726294 B CN102726294 B CN 102726294B CN 201210216732 CN201210216732 CN 201210216732 CN 201210216732 A CN201210216732 A CN 201210216732A CN 102726294 B CN102726294 B CN 102726294B
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- 238000000034 method Methods 0.000 title claims abstract description 18
- 244000058281 Ulmus pumila Species 0.000 title abstract 10
- 235000001547 Ulmus pumila Nutrition 0.000 title abstract 10
- 230000015784 hyperosmotic salinity response Effects 0.000 title abstract 9
- 102000018997 Growth Hormone Human genes 0.000 claims abstract description 13
- 108010051696 Growth Hormone Proteins 0.000 claims abstract description 13
- 239000000122 growth hormone Substances 0.000 claims abstract description 13
- 239000000654 additive Substances 0.000 claims abstract description 12
- 241001149163 Ulmus americana Species 0.000 claims description 60
- 150000003839 salts Chemical class 0.000 claims description 47
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical group C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 claims description 27
- 238000012549 training Methods 0.000 claims description 27
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 19
- 229910052799 carbon Inorganic materials 0.000 claims description 19
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 16
- 229930006000 Sucrose Natural products 0.000 claims description 16
- YGGXZTQSGNFKPJ-UHFFFAOYSA-N methyl 2-naphthalen-1-ylacetate Chemical compound C1=CC=C2C(CC(=O)OC)=CC=CC2=C1 YGGXZTQSGNFKPJ-UHFFFAOYSA-N 0.000 claims description 15
- 238000011081 inoculation Methods 0.000 claims description 14
- 241000196324 Embryophyta Species 0.000 claims description 8
- 230000032823 cell division Effects 0.000 claims description 8
- 235000021552 granulated sugar Nutrition 0.000 claims description 3
- 239000002609 medium Substances 0.000 abstract description 14
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 239000012882 rooting medium Substances 0.000 abstract description 3
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 abstract 1
- 239000004062 cytokinin Substances 0.000 abstract 1
- VGKONPUVOVVNSU-UHFFFAOYSA-N naphthalen-1-yl acetate Chemical compound C1=CC=C2C(OC(=O)C)=CC=CC2=C1 VGKONPUVOVVNSU-UHFFFAOYSA-N 0.000 description 12
- 238000010923 batch production Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 241000218220 Ulmaceae Species 0.000 description 1
- 241001106462 Ulmus Species 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 238000005034 decoration Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000003317 industrial substance Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
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- 239000008107 starch Substances 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The purpose of the present invention is to disclose an in-bottle rooting method for a salt tolerance ulmus pumila tissue culture seedling. The method comprises the following steps: (1) selecting a secondary seedling, wherein a secondary culture salt tolerance ulmus pumila tissue culture seedling is selected, the height of the seedling is 2-3.5 cm, and the robust individual ulmus pumila seedling having extending leaves is selected; and (2) inoculating the selected secondary culture salt tolerance ulmus pumila tissue culture seedling onto a WPM rooting medium, wherein the WPM rooting medium is added with growth hormone, cytokinin and exogenous additives. According to the present invention, a medium formula having a high rooting rate is adopted so as to effectively solve the rooting problem of the salt tolerance ulmus pumila tissue culture seedling, improve the rooting rate of the salt tolerance ulmus pumila tissue culture seedling, reduce the cost, accelerate the factory production of the salt tolerance ulmus pumila, provide high quality salt tolerance ulmus pumila seedlings for nursery stock markets, and achieve the purpose of the present invention, wherein the rooting rate of the salt tolerance ulmus pumila tissue culture seedling can be up to 95%.
Description
Technical field
The present invention relates to a kind of interior rooting method of bottle of training seedling, particularly rooting method in a kind of bottle of salt tolerant American elm group training seedling.
Background technology
American elm also claims elm, and the Ulmaceae deciduous tree is distributed more widely, the main product north temperate zone.American elm happiness light, drought-enduring, cold-resistant, impoverishment tolerant are not selected soil, and strong adaptability is multiplex in afforestation.Wear-resisting, the corrosion resistant of American elm timber is the good timber of shipbuilding, building, interior decoration floor, furniture.Bark fiber is tough, can do synthetic cotton and paper making raw material.Starch-containing and the protein of blade can be made feed.Skin, leaf, fruit can be used as medicine, and seed can extract oil, and is medicine and industrial chemicals.
American elm is generally used seminal propagation, but after the sexual propagation of good kind process, good characteristic can go down, for better keeping the merit of kind, must carry out vegetative propagation, keep the good characteristic of kind by the means of grafting, but cost is higher, and survival rate is lower.
Simultaneously, because American elm has certain Salt And Alkali Tolerance characteristic, the comparatively difficulty of taking root, rooting rate is lower, causes nursery stock market the present situation of supply-less-than-demand salt tolerant American elm seedling to be occurred.
Therefore, need especially a kind of interior rooting method of bottle of salt tolerant American elm group training seedling, solved the problem of above-mentioned existing existence.
Summary of the invention
The object of the present invention is to provide a kind of interior rooting method of bottle of salt tolerant American elm group training seedling, for the deficiencies in the prior art, effectively solved the problem of taking root of salt tolerant American elm group training seedling, improved the rooting rate of salt tolerant American elm group training seedling, reduced cost, accelerated the batch production production of salt tolerant American elm, the salt tolerant American elm seedling of high-quality is provided for nursery stock market.
Technical problem solved by the invention can realize by the following technical solutions:
Rooting method in a kind of bottle of salt tolerant American elm group training seedling is characterized in that it comprises the steps:
(1) the subculture seedling chooses
Choose the salt tolerant American elm group training seedling that subculture is cultivated, the individual plant American elm seedling that selection standard is unfolded for height 2-3.5cm, stalwartness, blade;
(2) the salt tolerant American elm tissue culture plant inoculation of the subculture of choosing being cultivated is to the WPM root media;
Be added with growth hormone, the basic element of cell division and additives in the described WPM root media.
In one embodiment of the invention, described growth hormone is indolebutyric acid (IBA) and methyl α-naphthyl acetate (NAA), described indolebutyric acid (IBA) to final concentration be 0.05-0.3mg/L; Described methyl α-naphthyl acetate (NAA) to final concentration be 0.05-0.2mg/L.
Further, preferably, described indolebutyric acid (IBA) to final concentration be 0.2mg/L, described methyl α-naphthyl acetate (NAA) to final concentration be 0.1mg/L.
In one embodiment of the invention, described additives is active carbon (AC) and white sugar, described active carbon (AC) to final concentration be 0.2-2g/L, described white sugar to final concentration be 20-40g/L.
Further, preferably, described active carbon to final concentration be 0.5g/L, described white granulated sugar to final concentration be 30g/L.
In one embodiment of the invention, the pH value of described WPM root media is 5.8-5.9.
In one embodiment of the invention, described WPM root media is for carrying out the WPM medium of decrement with the macroelement in the WPM minimal medium, and its decrement scope is 0-1/2.
Further, preferably, described WPM root media is the 1/2WPM medium.
Rooting method in the bottle of salt tolerant American elm group training seedling of the present invention, compared with prior art, adopted the culture medium prescription of higher rooting rate, effectively solved the problem of taking root of salt tolerant American elm group training seedling, improved the rooting rate of salt tolerant American elm group training seedling, salt tolerant American elm group training seedling rooting rate reaches as high as 95%, reduced cost, accelerate the batch production production of salt tolerant American elm, for nursery stock market provides the salt tolerant American elm seedling of high-quality, realized purpose of the present invention.
Characteristics of the present invention can be consulted the detailed description of the graphic and following better embodiment of this case and be obtained to be well understood to.
Description of drawings
Fig. 1 is the schematic diagram of the situation of taking root of the seedling of taking root in the observation salt tolerant American elm bottle at the bottom of blake bottle;
Fig. 2 is the schematic diagram of the situation of taking root of seedling of taking root in the right-angle view salt tolerant American elm bottle.
Embodiment
For technological means, creation characteristic that the present invention is realized, reach purpose and effect is easy to understand, below in conjunction with concrete diagram, further set forth the present invention.
Rooting method in the bottle of salt tolerant American elm group training seedling of the present invention, it comprises the steps:
(1) the subculture seedling chooses
Choose the salt tolerant American elm group training seedling that subculture is cultivated, the individual plant American elm seedling that selection standard is unfolded for height 2-3.5cm, stalwartness, blade;
(2) the salt tolerant American elm tissue culture plant inoculation of the subculture of choosing being cultivated is to the WPM root media;
Be added with growth hormone, the basic element of cell division and additives in the described WPM root media.
In the present invention, described growth hormone is indolebutyric acid (IBA) and methyl α-naphthyl acetate (NAA), described indolebutyric acid (IBA) to final concentration be 0.05-0.3mg/L; Described methyl α-naphthyl acetate (NAA) to final concentration be 0.05-0.2mg/L.
Preferably, described indolebutyric acid (IBA) to final concentration be 0.2mg/L, described methyl α-naphthyl acetate (NAA) to final concentration be 0.1mg/L.
In the present invention, described additives is active carbon (AC) and white sugar, described active carbon (AC) to final concentration be 0.2-2g/L, described white sugar to final concentration be 20-40g/L.
Preferably, described active carbon to final concentration be 0.5g/L, described white granulated sugar to final concentration be 30g/L.
In the present invention, the pH value of described WPM root media is 5.8-5.9.
In the present invention, described WPM root media is for carrying out the WPM medium of decrement with the macroelement in the WPM minimal medium, and its decrement scope is 0-3/4.
Preferably, described WPM root media is the 1/2WPM medium.
Embodiment 1
Choose the salt tolerant American elm group training seedling that subculture is cultivated, the individual plant American elm seedling that selection standard is unfolded for height 2-3.5cm, stalwartness, blade;
The salt tolerant American elm tissue culture plant inoculation that the subculture chosen is cultivated is to the WPM root media;
Wherein, described WPM root media is to be the 1/2WPM root media that carries out 1/2 decrement with the macroelement in the WPM minimal medium.
Described growth hormone is indolebutyric acid (IBA), its to final concentration be 0.05mg/L; The described basic element of cell division is methyl α-naphthyl acetate (NAA), its to final concentration be 0.05mg/L; Described additives is active carbon (AC) and white sugar, described active carbon (AC) to final concentration be 0.2g/L, described white sugar to final concentration be 20g/L.
The pH value of described WPM root media is 5.8.
Inoculate after 25 days, the statistics rooting rate, the rooting rate=strain number of taking root/inoculation strain number * 100%, its rooting rate is 80%.
Choose the salt tolerant American elm group training seedling that subculture is cultivated, the individual plant American elm seedling that selection standard is unfolded for height 2-3.5cm, stalwartness, blade;
The salt tolerant American elm tissue culture plant inoculation that the subculture chosen is cultivated is to the WPM root media;
Wherein, described WPM root media is to be the 1/2WPM root media that carries out 1/2 decrement with the macroelement in the WPM minimal medium.
Described growth hormone is indolebutyric acid (IBA), its to final concentration be 0.2mg/L; The described basic element of cell division is methyl α-naphthyl acetate (NAA), its to final concentration be 0.1mg/L; Described additives is active carbon (AC) and white sugar, described active carbon (AC) to final concentration be 0.5g/L, described white sugar to final concentration be 30g/L.
The pH value of described WPM root media is 5.85.
Inoculate after 25 days, the statistics rooting rate, the rooting rate=strain number of taking root/inoculation strain number * 100%, its rooting rate is 95%.
Embodiment 3
Choose the salt tolerant American elm group training seedling that subculture is cultivated, the individual plant American elm seedling that selection standard is unfolded for height 2-3.5cm, stalwartness, blade;
The salt tolerant American elm tissue culture plant inoculation that the subculture chosen is cultivated is to the WPM root media;
Wherein, described WPM root media is to be the 1/2WPM root media that carries out 1/2 decrement with the macroelement in the WPM minimal medium.
Described growth hormone is indolebutyric acid (IBA), its to final concentration be 0.3mg/L; The described basic element of cell division is methyl α-naphthyl acetate (NAA), its to final concentration be 0.2mg/L; Described additives is active carbon (AC) and white sugar, described active carbon (AC) to final concentration be 2g/L, described white sugar to final concentration be 40g/L.
The pH value of described WPM root media is 5.9.
Inoculate after 25 days, the statistics rooting rate, the rooting rate=strain number of taking root/inoculation strain number * 100%, its rooting rate is 85%.
Embodiment 4
Choose the salt tolerant American elm group training seedling that subculture is cultivated, the individual plant American elm seedling that selection standard is unfolded for height 2-3.5cm, stalwartness, blade;
The salt tolerant American elm tissue culture plant inoculation that the subculture chosen is cultivated is to the WPM root media;
Wherein, described WPM root media is to be the 3/4WPM root media that carries out 1/4 decrement with the macroelement in the WPM minimal medium.
Described growth hormone is indolebutyric acid (IBA), its to final concentration be 0.2mg/L; The described basic element of cell division is methyl α-naphthyl acetate (NAA), its to final concentration be 0.1mg/L; Described additives is active carbon (AC) and white sugar, described active carbon (AC) to final concentration be 0.5g/L, described white sugar to final concentration be 30g/L.
The pH value of described WPM root media is 5.8.
Inoculate after 25 days, the statistics rooting rate, the rooting rate=strain number of taking root/inoculation strain number * 100%, its rooting rate is 85%.
Embodiment 5
Choose the salt tolerant American elm group training seedling that subculture is cultivated, the individual plant American elm seedling that selection standard is unfolded for height 2-3.5cm, stalwartness, blade;
The salt tolerant American elm tissue culture plant inoculation that the subculture chosen is cultivated is to the WPM root media;
Wherein, described WPM root media is to be 1WPM root media with in the WPM minimal medium.
Described growth hormone is indolebutyric acid (IBA), its to final concentration be 0.3mg/L; The described basic element of cell division is methyl α-naphthyl acetate (NAA), its to final concentration be 0.15mg/L; Described additives is active carbon (AC) and white sugar, described active carbon (AC) to final concentration be 2g/L, described white sugar to final concentration be 40g/L.
The pH value of described WPM root media is 5.9.
Inoculate after 25 days, the statistics rooting rate, the rooting rate=strain number of taking root/inoculation strain number * 100%, its rooting rate is 80%.
Prescription of rooting medium is as shown in table 1, and medium is the WPM root media of improvement, and growth hormone is indolebutyric acid (IBA), methyl α-naphthyl acetate (NAA).Inoculate after 25 days statistics rooting rate, the rooting rate=strain number of taking root/inoculation strain number * 100%.
The orthogonal experiment of table 1 salt tolerant American elm root media screening
By the screening to salt tolerant American elm root media, finishing screen is selected and is fit to the medium that the salt tolerant American elm takes root, on described WPM root media, salt tolerant American elm rooting rate is higher, reach as high as 95%, and root is sturdy, fibrous root more (referring to Fig. 1 and Fig. 2), in the acclimatization and transplants process, improved the survival rate of American elm group training seedling, the present invention has not only improved the low problem of salt tolerant American elm rooting rate, and greatly reduces production cost, accelerate the progress that the batch production of salt tolerant American elm is produced, brought more wide economic benefit.
More than show and described basic principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; that describes in above-described embodiment and the specification just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications; these changes and improvements all fall in the claimed scope of the invention, and the claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (5)
1. rooting method in the bottle of a salt tolerant American elm group training seedling is characterized in that it comprises the steps:
(1) the subculture seedling chooses
Choose the salt tolerant American elm group training seedling that subculture is cultivated, the individual plant American elm seedling that selection standard is unfolded for height 2-3.5cm, stalwartness, blade;
(2) the salt tolerant American elm tissue culture plant inoculation of the subculture of choosing being cultivated is to the WPM root media;
Be added with growth hormone, the basic element of cell division and additives in the described WPM root media;
Described growth hormone is indolebutyric acid and methyl α-naphthyl acetate, described indolebutyric acid to final concentration be 0.05-0.3mg/L; Described methyl α-naphthyl acetate to final concentration be 0.05-0.2mg/L;
Described additives is active carbon and white sugar, described active carbon to final concentration be 0.2-2g/L, described white sugar to final concentration be 20-40g/L;
Described WPM root media is for carrying out the WPM medium of decrement with the macroelement in the WPM minimal medium, its decrement scope is 0-1/2.
2. rooting method in the bottle of salt tolerant American elm as claimed in claim 1 group training seedling is characterized in that, described indolebutyric acid to final concentration be 0.2mg/L, described methyl α-naphthyl acetate to final concentration be 0.1mg/L.
3. rooting method in the bottle of salt tolerant American elm as claimed in claim 1 group training seedling is characterized in that, described active carbon to final concentration be 0.5g/L, described white granulated sugar to final concentration be 30g/L.
4. rooting method in the bottle of salt tolerant American elm group training seedling as claimed in claim 1 is characterized in that the pH value of described WPM root media is 5.8-5.9.
5. rooting method in the bottle of salt tolerant American elm group training seedling as claimed in claim 1 is characterized in that described WPM root media is the 1/2WPM medium.
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Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102934612B (en) * | 2012-11-23 | 2014-02-26 | 上海杉一植物科技有限公司 | Subculture method of salt-tolerance ulmus pumila tissue culture seedlings |
| CN103734018B (en) * | 2014-01-23 | 2015-03-04 | 西北农林科技大学 | Subculture medium for saline/alkaline-tolerant fast-growing ulmus pumila tissue culture seedling |
| CN103749306B (en) * | 2014-01-23 | 2015-03-25 | 山东省林业科学研究院 | Rapid in-vitro propagation method of saline alkali tolerant fast-growing Ulmus pumila |
| CN104429958B (en) * | 2014-11-26 | 2016-08-24 | 上海杉一植物科技有限公司 | The method of rejuvenation in American elm tissue cultured seedling bottle |
| CN104663448A (en) * | 2015-03-01 | 2015-06-03 | 陈凤佳 | Tissue culture and rapid propagation technology for ulmus pumila Linn. |
| CN105494108B (en) * | 2016-02-29 | 2017-12-12 | 山东省林木种质资源中心 | A kind of tissue culture and rapid propagation method of fast-growing American elm |
| CN106106172A (en) * | 2016-07-26 | 2016-11-16 | 象山宏森源农产品开发有限公司 | A kind of Salt And Alkali Tolerance elm tissue culture propagation technology |
| CN108308023B (en) * | 2018-01-15 | 2020-03-20 | 山东建筑大学 | Saline-alkali-tolerant fast-growing Ulmus pumila clone adventitious bud tissue culture rooting method |
| CN110338064B (en) * | 2019-08-29 | 2021-04-06 | 上海杉一植物科技有限公司 | Rooting culture and seedling raising method for ulmus davidiana tissue culture seedlings |
| CN113207695A (en) * | 2021-06-17 | 2021-08-06 | 江苏省林业科学研究院 | Formula for tissue culture of ulmus parvifolia |
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