CN101984787B - Method for strengthening tissue culture seedlings of rhododendron lapponicum - Google Patents
Method for strengthening tissue culture seedlings of rhododendron lapponicum Download PDFInfo
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- CN101984787B CN101984787B CN2010105377330A CN201010537733A CN101984787B CN 101984787 B CN101984787 B CN 101984787B CN 2010105377330 A CN2010105377330 A CN 2010105377330A CN 201010537733 A CN201010537733 A CN 201010537733A CN 101984787 B CN101984787 B CN 101984787B
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Abstract
The invention relates to the field of rhododendron lapponicum propagation. In the technical scheme adopted by the invention, a method for strengthening tissue culture seedlings of rhododendron lapponicum comprises the following steps of: selecting the tissue culture seedlings having the height of about 5 to 8cm as test materials and culturing the tissue culture seedlings on a special culture medium of AD rhododendron lapponicum without any added hormone for one week; and then inoculating the tissue culture seedlings into a seedling strengthening and rooting culture medium, wherein the culturing temperature is 21+/-2DEG C, the illumination intensity is 2,500Lx, and the illumination time is 16 hours each day; and the seedling strengthening and rooting culture medium is an AD culture medium of which each litre is added with 0.1 to 1.5mg of IBA, 30g of cane sugar, 10 to 30mmol of 2-(N-morpholine) ethanesulfonic acid and 0.1 to 0.6g of active carbon. The method lays a foundation for transplanting the tissue culture seedlings of the rhododendron lapponicum and reduces the production cost at the same time.
Description
Technical field
The present invention relates to the method for propagation field; Particularly alpine rose breeds the field.
Background technology
Alpine rose (Rhododendron lapponicum) Ericaceae cuckoo belongs to, and its inflorescence is big, the four seasons are evergreen, floral leaf all has higher ornamental value, has huge market development space.But the alpine rose breeding is difficulty, mainly is very difficulty of cuttage root-taking; China alpine rose potted flower almost all depends on import.Though at present domestic also have pair alpine rose tissue culture expanding propagation technology to explore; But because the phenomenon that seedling is elongated, the little color of leaf is light green, stem is easy to break appears in alpine rose tissue cultivating seedling easily in reproductive process; Be unfavorable for that tissue cultivating seedling takes root; It is very low to transplant the back survival rate, has greatly limited the batch production production of alpine rose.
Summary of the invention
The present invention provides a kind of alpine rose tissue cultivating seedling method in strong sprout for solving the excessive growth of alpine rose tissue cultivating seedling, the difficulty of taking root and cost high-technology problem, to improve the quality of tissue cultivating seedling, is convenient to the transplanting of tissue cultivating seedling.
The present invention is that the technical scheme of being taked is: the method in alpine rose tissue cultivating seedling strong sprout; Selecting the tissue cultivating seedling of high about 5～8cm is test material, earlier tissue cultivating seedling is cultivated for 1 week in the AD that does not add any hormone (Athanasios and Read) the cuckoo special culture media sky; Be transferred to then on the strengthening seedling and rooting medium; Condition of culture is 21 ± 2 ℃ of temperature, intensity of illumination 2500Lx, and every day, light application time was 16h; Described strengthening seedling and rooting medium is every liter of additional 0.1～1.5mg IBA, sucrose 30g, 10～30mmol 2-(N-morpholine) ethyl sulfonic acid, the AD medium of 0.1～0.6g active carbon.
It is light source that the red LED lamp of 40W is adopted in described illumination.
Advantage and good effect that the present invention has are: adopt the method in strong sprout of alpine rose of the present invention, plant is shorter, and cane is more sturdy; Blade is bottle green, and vein is obvious, and is glossy, and leaf area has increased by 4 times; The adventive root that produces is more, and rooting rate has increased nearly one times.For the transplanting of alpine rose tissue cultivating seedling lays the foundation.Also reduced simultaneously production cost.
Embodiment
The experimental group test method: selecting the tissue cultivating seedling of high about 5～8cm is test material, earlier tissue cultivating seedling is cultivated for 1 week in the AD that does not add any hormone (Athanasios and Read) the cuckoo special culture media sky; Be transferred to then on the strengthening seedling and rooting medium; Condition of culture is 21 ± 2 ℃ of temperature, intensity of illumination 2500Lx, and every day, light application time was 16h; Described strengthening seedling and rooting medium is every liter of additional 0.1～1.5mg IBA, sucrose 30g, 10～30mmol 2-(N-morpholine) ethyl sulfonic acid, the AD medium of 0.1～0.6g active carbon.
Experimental group 1
Adopt said method, described strengthening seedling and rooting medium is every liter of additional 0.1mg IBA, 10mg 2-(N-morpholine) ethyl sulfonic acid, the AD medium of 0.1mg active carbon.
Experimental group 2
Adopt said method, described strengthening seedling and rooting medium is every liter of additional 0.5mg IBA, 20mg 2-(N-morpholine) ethyl sulfonic acid, the AD medium of 0.3mg active carbon.
Experimental group 3
Described strengthening seedling and rooting medium is every liter of additional 1.5mg IBA, 30mg 2-(N-morpholine) ethyl sulfonic acid, the AD medium of 0.6mg active carbon.
Control group: selecting the tissue cultivating seedling of high about 5～8cm is test material, earlier tissue cultivating seedling is cultivated for 1 week in the AD that does not add any hormone (Athanasios and Read) the cuckoo special culture media sky.Be transferred to every liter of additional 0.5mgIBA after empty the cultivation, sucrose 30g is on the strengthening seedling and rooting medium of 0.3g active carbon.It is light source that fluorescent lamp is adopted in illumination, and each group training frame is adorned the fluorescent lamp of 3 40W, its intensity of illumination 2000～3000Lx, illumination every day 16h.Cultivation temperature is 21 ± 2 ℃.
Interpretation of result: the alpine rose happiness is cool, and its cultivation temperature is than low 2～5 ℃ of other plant, and decrease of temperature causes medium osmotic pressure to reduce, and is unfavorable for the growth of tissue cultivating seedling.In this test, we add 10～30mmol 2-(N-morpholine) ethyl sulfonic acid at every liter of strengthening seedling and rooting medium, and back plant leaf is bigger, is bottle green, have reduced osmotic pressure and have changed the influence to the tissue cultivating seedling growth.
Alpine rose happiness light, the growth of its tissue cultivating seedling also needs stronger illumination, has greatly improved the cost of tissue cultivating seedling.This test adopts LED lamp (ruddiness) as light source, and plant is shorter, compares with the culture effect with fluorescent lamp as light source, and the number of blade has reduced but leaf area has increased by 3 times, and blade is bottle green, and vein is obvious, and is glossy.LED (ruddiness) lamp helps the alpine rose tissue cultivating seedling takes root, and rooting rate reaches 31.6%, has increased nearly one times.
Table 1 Different Light is to the influence of alpine rose effect in strong sprout
Claims (2)
1. the method in alpine rose tissue cultivating seedling strong sprout, it is characterized by: the tissue cultivating seedling of selecting high 5～8cm is a test material, earlier tissue cultivating seedling is cultivated for 1 week in that the AD cuckoo special culture media that does not add any hormone is overhead; Be transferred to then on the strengthening seedling and rooting medium; Condition of culture is 21 ± 2 ℃ of temperature, intensity of illumination 2500Lx, and every day, light application time was 16h; Described strengthening seedling and rooting medium is every liter of additional 0.1～1.5mg IBA, sucrose 30g, 10～30mmol 2-(N-morpholine) ethyl sulfonic acid, the AD medium of 0.1～0.6g active carbon.
2. the method in alpine rose tissue cultivating seedling according to claim 1 strong sprout, it is characterized by: it is light source that the red LED lamp of 40W is adopted in described illumination.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105377330A CN101984787B (en) | 2010-11-10 | 2010-11-10 | Method for strengthening tissue culture seedlings of rhododendron lapponicum |
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| CN2010105377330A CN101984787B (en) | 2010-11-10 | 2010-11-10 | Method for strengthening tissue culture seedlings of rhododendron lapponicum |
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| Publication Number | Publication Date |
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| CN101984787A CN101984787A (en) | 2011-03-16 |
| CN101984787B true CN101984787B (en) | 2012-01-04 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103125395B (en) * | 2013-03-18 | 2014-04-02 | 南京斯摩尼生物科技有限公司 | Culture medium for promoting adventive root of woody plant to root and grow and application of culture medium |
| CN103636492B (en) * | 2013-11-14 | 2015-07-08 | 石家庄市农林科学研究院 | Rhododendron hybrides cosmopolitan tissue culture rapid propagation method |
| CN105191766B (en) * | 2015-09-02 | 2017-09-05 | 云南省农业科学院花卉研究所 | A kind of quick hardening off method of alpine rose tissue-cultured seedling |
| CN114258857A (en) * | 2021-12-08 | 2022-04-01 | 天津农学院 | Comprehensive method for preventing and treating aging of tissue culture seedlings and application |
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