CN106171996A - A kind of method for quickly breeding of wild Betula platyphylla Suk. - Google Patents
A kind of method for quickly breeding of wild Betula platyphylla Suk. Download PDFInfo
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- CN106171996A CN106171996A CN201610576291.8A CN201610576291A CN106171996A CN 106171996 A CN106171996 A CN 106171996A CN 201610576291 A CN201610576291 A CN 201610576291A CN 106171996 A CN106171996 A CN 106171996A
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- wild
- betula platyphylla
- platyphylla suk
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention provides the method for quickly breeding of a kind of wild Betula platyphylla Suk., comprises the steps: 1) acquisition of the outer implant of wild Betula platyphylla Suk. stem section；2) inducing culture of the outer implant of wild Betula platyphylla Suk. stem section；3) enrichment culture of wild Betula platyphylla Suk. Multiple Buds；4) root culture of wild Betula platyphylla Suk.: be divided into 2 stages, the first stage: first cultivated at the first root media by the simple bud that Multiple Buds cuts, be placed in 2800～3000Lux, illumination every day 20h cultivation is until taking root 8～10d；Second stage: cultivate 20～30d in the second root media of transferring again, grow good root system；5) seedling exercising of wild Betula platyphylla Suk. tissue cultured seedling and transplanting: after room temperature seedling exercising 7～10d, is transplanted in substrate cultivation 30～35d, it is thus achieved that the Fast-propagation Seedling of wild Betula platyphylla Suk.；6) transplant, be transplanted to planting site.The present invention solves wild Betula platyphylla Suk. tissue culture and takes root difficulty, problem that rooting rate is low.
Description
Technical field
The present invention relates to the method for quickly breeding of a kind of wild Betula platyphylla Suk..
Background technology
Betula platyphylla Suk. (Bettlla platyphylla suk), belongs to Betulaceae Betula plant, deciduous tree, and height is up to 20-
25m, diameter of a cross-section of a tree trunk 1.3 meters above the ground 50cm, bark white, smooth, paper-like layering is peeled off；The brown bronzing of twig, leaf triangular shape is avette, and tip is tapering, base
Portion cuts shape, and pyrene is flat, both sides film quality wing, blooms the 5-6 month, and August, fruit was ripe.
Betula platyphylla Suk. originates in Manchurian Daxing'an Mountainrange, the Xiaoxinanlin Mountains, Changbai Mountain and alpine region, North China and the A Er of northwest
Mount Taishan；Be distributed in China's northeast Xing'anling mountains, Changbai Mountain, the Shanxi in North China, Hunan, Hubei, the Inner Mongol of northwest, Ningxia,
Shaanxi, Qinghai, Tibet, the Sichuan in southwest, northwestern Yunnan Province.The Betula platyphylla Suk. happiness moistening rich soil of light happiness, resistance to dry lean, grow rapidly,
Life-span is short, and rudiment power is strong, is one of main vanguard tree seed forming scondary forest after forest deterioration, is also temperate zone broad-leaf forest and broad-leaved
The chief species of mixed forest.Betula platyphylla Suk. texture is straight, and structure is thin, in hardness, is good sawing raw material and disposable chopsticks processing
Raw material, it is possible to as pulpwood, glued material, construction timber and general civilian material, bark can extract Betula platyphylla Suk. oil, and Betula platyphylla Suk. juice can be opened
Sending out beverage, leaf is the most pharmaceutically acceptable, is also nectariferous plant.
Betula platyphylla Suk. branch fine and soft sagging, attitude is graceful, smooth pure white, as shade tree and Garden virescence tree, there is uniqueness
Ornamental plantation is worth.Improve the wild Betula platyphylla Suk. breeding shortcut as a fast-developing greening, more come in urban landscaping
More favored by people, improved urban green space landscape in a short time, improve the ecological benefits of urban green space, optimize greenery patches knot
Structure, promote that the aspect such as formation in advance of urban ecological environment plays a positive role.At present, Betula platyphylla Suk. has been listed in two grades of country
Protection plant and the plant protection resource of emphasis, and, Betula platyphylla Suk. is slow mainly by seminal propagation and reproduction speed, it is difficult to meet city
Field demand.Therefore, by studying to expand stock number to Betula platyphylla Suk. Fast-propagation, it is possible to resolve imbalance between supply and demand.
Summary of the invention
In order to solve the problems referred to above, the present invention provides the method for quickly breeding of a kind of wild Betula platyphylla Suk., uses at the technology of the present invention
Managing the breeding of wild Betula platyphylla Suk., can improve wild Betula platyphylla Suk. reproductive survival rate, survival rate can reach more than 98%, and has operation letter
Single, chemical raw material kind and consumption is few, the short and feature of low cost that processes the time.
The technical solution used in the present invention is:
The method for quickly breeding of a kind of wild Betula platyphylla Suk., comprises the steps:
1) acquisition of the outer implant of wild Betula platyphylla Suk. stem section: the stem section that intercepting 6cm length contains bud from wild Betula platyphylla Suk. bearing tree is
Outer implant, after outer implant disinfection, aseptic water washing, suck dry moisture, is cut into the stem section with an axillalry bud by branch section；
2) inducing culture of the outer implant of wild Betula platyphylla Suk. stem section: the outer implant obtained is carried out inducing culture, is 25 in temperature
～28 DEG C, intensity of illumination is 2200～2500Lux, illumination every day 14～16h, and after 40～45d, axillalry bud starts to sprout, and is formed little
Bud；
3) enrichment culture of wild Betula platyphylla Suk. Multiple Buds: the new long axillalry bud Seedling turned out aseptically is cut into only band one
The stem section of individual axillalry bud, access proliferated culture medium be 28～30 DEG C of cultivations in temperature, be placed in 2600～2800Lux illumination every day 18～
20h forms Multiple Buds after cultivating 25～30d；
4) root culture of wild Betula platyphylla Suk.: be divided into 2 stages, the first stage: the simple bud first cut by Multiple Buds is first
Root media is cultivated, and is placed in 2800～3000Lux, and illumination every day 20h cultivates until taking root 8～10d；Second stage: turn again
Connect and the second root media is cultivated 20～30d, grow good root system；
5) seedling exercising of wild Betula platyphylla Suk. tissue cultured seedling and transplanting: after room temperature seedling exercising 7～10d, be transplanted in substrate cultivation 30～
35d, it is thus achieved that the Fast-propagation Seedling of wild Betula platyphylla Suk.；
6) transplant, be transplanted to planting site.
The method for quickly breeding of described a kind of wild Betula platyphylla Suk., step 2) inducing culture based component is in inducing culture: WPM
+ 6-BA 1.5～2.0mg/L+NAA 1.0～2.0mg/L+ sucrose 40g/L+ agar 5g/L, pH5.5～6.5.
The method for quickly breeding of described a kind of wild Betula platyphylla Suk., step 3) in the medium component of proliferated culture medium be: MS+
NAA 1.0～2.0mg/L+IBA 0.05～0.2mg/L+ vitamin B61.5～2.0mg/L+GA31.5～2.0mg/L+ sucrose
40g/L+ agar 5g/L, pH 5.5～6.5.
The method for quickly breeding of described a kind of wild Betula platyphylla Suk., step 4) described in the first root culture based component be: 1/
4MS+IBA 1.0～1.5mg/L+ fishbone powder 2.0～2.5g/L+ vitamin B11.5～2.0mg/L+ sucrose 35g/L+ agar
5g/L, pH 5.5～6.5.
The method for quickly breeding of described a kind of wild Betula platyphylla Suk., step 4) described in the second root culture based component be: 1/
2MS+NAA 0.5～1.0mg/L+ fishbone powder 2.0～2.5g/L+ vitamin B11.5～2.0mg/L+GA31.5～2.0mg/L+
Sucrose 35g/L+ agar 5g/L, pH 5.5～6.5.
The method for quickly breeding of described a kind of wild Betula platyphylla Suk., step 6) transplant, particularly as follows: when seedling lateral root number reaches
8～10, when main root length reaches 15cm, start transplanting and to the big Tanaka of 60 × 60cm line space or plant into height × a diameter of 30
In the container of × 35cm, it is trained the container seedling of height 90～100cm, is transplanted to planting site.
The method have the advantages that
1. inducing culture in the present invention, enrichment culture, the culture medium of root culture preparation, it is thus achieved that seedling take root easily,
Healthy and strong degeneration-resistant, resistant to diseases and insects is strong, and seedling neat and consistent, and quality is high, and cultivation management is easier to.Utilize the group that the present invention obtains
Seedlings cultivating, adaptable to urban environment, solve wild Betula platyphylla Suk. tissue culture and take root difficulty, problem that rooting rate is low.
2. the material processing the use of wild Betula platyphylla Suk. is simple.
3. high treating effect: after process, wild Betula platyphylla Suk. survival rate can reach more than 98%.
4. low cost: whole process technique has only to several frequently seen chemical drugs.
5. recruitment is few: processes wild Betula platyphylla Suk. and has only to 3-5 workman.
6. technique is simple, be easy to grasp.
7. it is easy to mass production: owing to low cost, technique are simple, it is simple to mass production.
8. pollute lighter: process the remaining medicinal liquid of wild Betula platyphylla Suk. every time and can add the use of medicine Posterior circle.
9. provide advanced technical support for the breeding of the wild Betula platyphylla Suk. in areas of Shenyang.
Detailed description of the invention
Embodiment 1, the method for quickly breeding of a kind of wild Betula platyphylla Suk.
(1) method is as follows:
1) acquisition of the outer implant of wild Betula platyphylla Suk. stem section:
Intercepting the 6cm length stem section that contains bud from wild Betula platyphylla Suk. bearing tree is outer implant, by outer implant disinfection,
After aseptic water washing, suck dry moisture, branch section is cut into the stem section with an axillalry bud；
2) inducing culture of the outer implant of wild Betula platyphylla Suk. stem section:
The outer implant obtained being carried out inducing culture, is 25～28 DEG C in temperature, intensity of illumination is 2200～2500Lux,
Illumination every day 14～16h, after 40～45d, axillalry bud starts to sprout, and forms budlet；Described inducing culture based component is: WPM+6-BA
1.5～2.0mg/L+NAA 1.0～2.0mg/L+ sucrose 40g/L+ agar 5g/L, pH5.5～6.5；
3) enrichment culture of wild Betula platyphylla Suk. Multiple Buds:
The new long axillalry bud Seedling turned out aseptically is cut into the stem section only with an axillalry bud, accesses proliferated culture medium
It is 28～30 DEG C of cultivations in temperature, is placed in 2600～2800Lux illumination every day 18～20h, formed after cultivating 25～30d and grow thickly
Bud；Described enrichment culture based component is: MS+NAA 1.0～2.0mg/L+IBA 0.05～0.2mg/L+ vitamin B61.5～
2.0mg/L+GA31.5～2.0mg/L+ sucrose 40g/L+ agar 5g/L, pH 5.5～6.5；
4) root culture of wild Betula platyphylla Suk.: be divided into 2 stages,
First stage: first the simple bud that Multiple Buds cuts is cultivated at the first root media, be placed in 2800～3000Lux,
Illumination every day 20h cultivates until taking root 8～10d；
Second stage: cultivate 20～30d in the second root media of transferring again, grow good root system；Described first is raw
Root medium component is: 1/4MS+IBA 1.0～1.5mg/L+ fishbone powder 2.0～2.5g/L+ vitamin B11.5～2.0mg/L
+ sucrose 35g/L+ agar 5g/L, pH 5.5～6.5；Described second root culture based component is: 1/2MS+NAA 0.5～
1.0mg/L+ fishbone powder 2.0～2.5g/L+ vitamin B11.5～2.0mg/L+GA31.5～2.0mg/L+ sucrose 35g/L+ fine jades
Fat 5g/L, pH 5.5～6.5；
5) seedling exercising of wild Betula platyphylla Suk. tissue cultured seedling and transplanting:
After room temperature seedling exercising 7～10d, it is transplanted in substrate cultivation 30～35d, it is thus achieved that the Fast-propagation Seedling of wild Betula platyphylla Suk.；
6) transplant
When seedling lateral root number reaches 8～10, when main root length reaches 15cm, start to transplant to 60 × 60cm line space
Big Tanaka or plant the container of height × a diameter of 30 × 35cm, be trained the container seedling of height 90～100cm, be transplanted to plant
Plant ground.
Embodiment 2 contrast experiment
1, material selection:
The stem section that intercepting 6cm length contained bud from the wild Betula platyphylla Suk. bearing tree of 8～10 years is outer implant, outer implant is carried out
Disinfect, after aseptic water washing, suck dry moisture, branch section be cut into the stem section 80 with an axillalry bud, be randomly divided into 4 groups.
Group 1: the method using embodiment 1.
Group 2: identical with embodiment 1 method, difference is: the 2nd) in step, do not use the wild Betula platyphylla Suk. of the present invention
Method for quickly breeding, and use MS+6-BA 0.5～1.0mg/L+NAA 0.5～1.0mg/L+ sucrose 40g/L+ agar 5g/
L, pH5.5～6.5；
Group 3: identical with embodiment 1 method, difference is: the 3rd) in step, do not use the wild Betula platyphylla Suk. of the present invention
Method for quickly breeding, and use MS+NAA1.0～2.0mg/L+IBA0.05～0.2mg/L+ sucrose 40g/L+ agar 5g/L,
PH5.5～6.5；
Group 4: identical with embodiment 1 method, difference is: the 4th) in step, do not use the wild Betula platyphylla Suk. of the present invention
Method for quickly breeding described in the first root culture based component, and use: 1/4MS+IBA 1.0～1.5mg/L+ sucrose 35g/L+
Agar 5g/L, pH 5.5～6.5；
Group 5: identical with embodiment 1 method, difference is: the 4th) in step, do not use the wild Betula platyphylla Suk. of the present invention
Method for quickly breeding described in the second root culture based component, and use: 1/2MS+NAA 0.5～1.0mg/L+ fishbone powder 2.0
～2.5g/L+ vitamin B11.5～2.0mg/L+ sucrose 35g/L+ agar 5g/L, pH 5.5～6.5；
2, result
March of First Year transplants, and second and June of the 3rd year observe growing state, result such as table 1.
Table 1
From table 1,1. use the method wild Betula platyphylla Suk. reproductive survival rate of the present invention to reach 98%, can averagely improve survival rate
Reach more than 70%.High standard, high-quality for Landscaping of Northern City complete to provide guarantee.2. the raising of the present invention is wild
The Fast-propagation of Betula platyphylla Suk., can make the well developed root system of nursery stock, and side radical is many, and volume increase income is big.3. the raising wild white of the present invention
The method for quickly breeding Water-saving effect of birch is good, raw material is easy to get, small investment, instant effect, water saving saving of labor application.
Claims (6)
1. the method for quickly breeding of a wild Betula platyphylla Suk., it is characterised in that comprise the steps:
1) acquisition of the outer implant of wild Betula platyphylla Suk. stem section: the stem section that intercepting 6cm length contains bud from wild Betula platyphylla Suk. bearing tree is outer planting
Body, after outer implant disinfection, aseptic water washing, suck dry moisture, is cut into the stem section with an axillalry bud by branch section；
2) inducing culture of the outer implant of wild Betula platyphylla Suk. stem section: the outer implant obtained is carried out inducing culture, is 25～28 in temperature
DEG C, intensity of illumination is 2200～2500Lux, illumination every day 14～16h, and after 40～45d, axillalry bud starts to sprout, and forms budlet；
3) enrichment culture of wild Betula platyphylla Suk. Multiple Buds: the new long axillalry bud Seedling turned out aseptically is cut into only one axil of band
The stem section of bud, accessing proliferated culture medium is 28～30 DEG C of cultivations in temperature, is placed in 2600～2800Lux illumination every day 18～20h
Multiple Buds is formed after cultivating 25～30d；
4) root culture of wild Betula platyphylla Suk.: be divided into 2 stages, the first stage: first the simple bud that Multiple Buds cuts is taken root first
Culture medium culturing, is placed in 2800～3000Lux, and illumination every day 20h cultivates until taking root 8～10d；Second stage: transfer again
Two root medias are cultivated 20～30d, grows good root system；
5) seedling exercising of wild Betula platyphylla Suk. tissue cultured seedling and transplanting: after room temperature seedling exercising 7～10d, is transplanted in substrate cultivation 30～35d, obtains
Obtain the Fast-propagation Seedling of wild Betula platyphylla Suk.；
6) transplant, be transplanted to planting site.
The method for quickly breeding of a kind of wild Betula platyphylla Suk. the most as claimed in claim 1, it is characterised in that step 2) in inducing culture
Inducing culture based component is: WPM+6-BA 1.5～2.0mg/L+NAA 1.0～2.0mg/L+ sucrose 40g/L+ agar 5g/L,
PH5.5～6.5.
The method for quickly breeding of a kind of wild Betula platyphylla Suk. the most as claimed in claim 1, it is characterised in that step 3) in enrichment culture
The medium component of base is: MS+NAA 1.0～2.0mg/L+IBA 0.05～0.2mg/L+ vitamin B61.5～2.0mg/L+
GA31.5～2.0mg/L+ sucrose 40g/L+ agar 5g/L, pH 5.5～6.5.
The method for quickly breeding of a kind of wild Betula platyphylla Suk. the most as claimed in claim 1, it is characterised in that step 4) described in first
Root culture based component is: 1/4MS+IBA 1.0～1.5mg/L+ fishbone powder 2.0～2.5g/L+ vitamin B11.5～
2.0mg/L+ sucrose 35g/L+ agar 5g/L, pH 5.5～6.5.
The method for quickly breeding of a kind of wild Betula platyphylla Suk. the most as claimed in claim 1, it is characterised in that step 4) described in second
Root culture based component is: 1/2MS+NAA 0.5～1.0mg/L+ fishbone powder 2.0～2.5g/L+ vitamin B11.5～
2.0mg/L+GA31.5～2.0mg/L+ sucrose 35g/L+ agar 5g/L, pH 5.5～6.5.
The method for quickly breeding of a kind of wild Betula platyphylla Suk. the most as claimed in claim 1, it is characterised in that step 6) transplant, specifically
For: when seedling lateral root number reaches 8～10, when main root length reaches 15cm, start to transplant to 60 × 60cm line space is big
Tanaka or plant the container of height × a diameter of 30 × 35cm, is trained the container seedling of height 90～100cm, is transplanted to plantation
Ground.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610576291.8A CN106171996B (en) | 2016-07-21 | 2016-07-21 | A kind of rapid propagation method of wild white birch |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610576291.8A CN106171996B (en) | 2016-07-21 | 2016-07-21 | A kind of rapid propagation method of wild white birch |
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| CN106171996A true CN106171996A (en) | 2016-12-07 |
| CN106171996B CN106171996B (en) | 2018-02-09 |
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ID=57491041
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| CN201610576291.8A Active CN106171996B (en) | 2016-07-21 | 2016-07-21 | A kind of rapid propagation method of wild white birch |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108633737A (en) * | 2018-05-15 | 2018-10-12 | 句容市茂润苗木有限公司 | A kind of method for culturing seedlings of cherry tree |
| CN108703071A (en) * | 2018-05-18 | 2018-10-26 | 句容市茂润苗木有限公司 | A kind of white poplar fast seedling-cultivating method |
| CN112189564A (en) * | 2020-11-03 | 2021-01-08 | 毕节市林业科学研究所 | Method for propagating betula luminifera aseptic seedlings by tissue culture technology |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06303868A (en) * | 1993-04-20 | 1994-11-01 | Sumitomo Ringyo Kk | Method for mass proliferation of betula platyphylla sukatchev var. japonica hara |
| CN101502237A (en) * | 2009-01-24 | 2009-08-12 | 东北林业大学 | Micro-propagation method for white birch with purple leaf |
| RU2009143124A (en) * | 2009-11-24 | 2011-05-27 | Российская Федерация в лице Федерального агентства по науке и инновациям (RU) | BINARY VECTOR pBI-4CL, BINARY VECTOR pBI-Xeg, BINARY VECTOR pGS, METHOD FOR PRODUCING TRANSGENIC PLANTS CONTAINING A REDUCED NUMBER OF LIGNIN, GREEN CRANBERRY |
| CN103125399A (en) * | 2013-03-20 | 2013-06-05 | 东北林业大学 | Rapid issue culture propagation method for white birch tetraploid |
| CN105684904A (en) * | 2016-02-03 | 2016-06-22 | 东北林业大学 | Method for preparing haploid regenerated plants from betulaplatyphylla anther by virtue of isolated culture |
-
2016
- 2016-07-21 CN CN201610576291.8A patent/CN106171996B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06303868A (en) * | 1993-04-20 | 1994-11-01 | Sumitomo Ringyo Kk | Method for mass proliferation of betula platyphylla sukatchev var. japonica hara |
| CN101502237A (en) * | 2009-01-24 | 2009-08-12 | 东北林业大学 | Micro-propagation method for white birch with purple leaf |
| RU2009143124A (en) * | 2009-11-24 | 2011-05-27 | Российская Федерация в лице Федерального агентства по науке и инновациям (RU) | BINARY VECTOR pBI-4CL, BINARY VECTOR pBI-Xeg, BINARY VECTOR pGS, METHOD FOR PRODUCING TRANSGENIC PLANTS CONTAINING A REDUCED NUMBER OF LIGNIN, GREEN CRANBERRY |
| CN103125399A (en) * | 2013-03-20 | 2013-06-05 | 东北林业大学 | Rapid issue culture propagation method for white birch tetraploid |
| CN105684904A (en) * | 2016-02-03 | 2016-06-22 | 东北林业大学 | Method for preparing haploid regenerated plants from betulaplatyphylla anther by virtue of isolated culture |
Non-Patent Citations (1)
| Title |
|---|
| 张学英等: ""白桦组织培养技术研究"", 《北方园艺》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108633737A (en) * | 2018-05-15 | 2018-10-12 | 句容市茂润苗木有限公司 | A kind of method for culturing seedlings of cherry tree |
| CN108703071A (en) * | 2018-05-18 | 2018-10-26 | 句容市茂润苗木有限公司 | A kind of white poplar fast seedling-cultivating method |
| CN112189564A (en) * | 2020-11-03 | 2021-01-08 | 毕节市林业科学研究所 | Method for propagating betula luminifera aseptic seedlings by tissue culture technology |
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| Publication number | Publication date |
|---|---|
| CN106171996B (en) | 2018-02-09 |
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