CN101176429A - Culture medium composition adapting for blueberry test tube plantlet radication as well as method thereof - Google Patents
Culture medium composition adapting for blueberry test tube plantlet radication as well as method thereof Download PDFInfo
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- CN101176429A CN101176429A CNA2007101571946A CN200710157194A CN101176429A CN 101176429 A CN101176429 A CN 101176429A CN A2007101571946 A CNA2007101571946 A CN A2007101571946A CN 200710157194 A CN200710157194 A CN 200710157194A CN 101176429 A CN101176429 A CN 101176429A
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Abstract
The invention relates to a culture medium composition suitable for the rooting of blue berry plantlets and the method. The procedures of the invention are that: first, the preparation of rooting medium and the weight of the rooting medium contained in every liter is that: firstly, a basic medium: 1/2WPM culture medium, wherein white sugar 20g/L, pH5.2 to 5.4; secondly, culture medium supporting substance: perlite, 150 to 200g/L; thirdly, the rooting medium: 1/2WPM+IBA 0.03 to 0.1mg/L. Second, the single plant cultivated for 40 to 50 days in a multiplication medium and having a length of more than 3 to 5 centimeters is inoculated to the rooting medium; after 20 to 25 days, a plurality of roots are grown at the basal of the seedling. Third, the domestication and the transplantation of tissue culture seedlings: the rooted tissue culture seedlings of the second step having a length of more than 3 to 7 centimeters are directly transplanted into a growing media without washing the root section. The invention has the advantages that: the provided blue berry rooting optimized medium has strong pertinence and good applicability; the culture medium supporting substance can be reused; the transplanting survival rate is more than 90%; running water and white sugar are used in the invention to replace distilled water and sucrose, therefore tissue culture cost is saved.
Description
Technical field
The present invention relates to field of plant tissue culture technique, mainly is a kind of culture media composition and method thereof of suitable blueberry rooting of vitro seedling.
Background technology
Blueberry is one of the most potential fruit tree species in the world wide in this century.Its fruit is because of being rich in the health care of anti-oxidant composition tool uniquenesses such as various vitamins, ursin, anthocyanin, SOD, tanning acid and flavonoids.In kind of the fruits and vegetables, the antioxidation activity of blue berry is the highest surplus 40, has anti-eye strain, improves eyesight, eliminates inflammation in the body, effects such as hemangiectasis; And urinary system, alimentary infection, cardiopathy, cerebral thrombus etc. are had tangible curative effect, play effects such as delaying the cerebral nerve aging, strengthen memory, be anticancer.Blue berry processing characteristics is splendid, its fruit soft and succulency, fragrance uniqueness, lovely luster and pigment are stable, suiting to be processed into high-grade products such as fruit juice, jam, fruit wine and natural colouring matter, health food, also is the important source material of antioxidant from natural food and natural drug.Rapid because of developing, the nurseries anxiety, and adopt method for plant tissue culture can breed various plants in a short time fast, reproduction rate height not only, and because it is vegetative propagation, can keep the merit of former stock, on producing, use more and more wider in recent years.But the complexity of different plant utilization method for plant tissue culture breeding seedling is different, have in the document of existing report that the blueberry rooting rate is low, the cycle of taking root is long, the program of taking root complexity (secretly earlier cultivates after 20 days light cultivation 20 days down, and rooting rate is 30%-70% only) and the problems such as survival rate is low of transplanting seedlings, make it can't realize large-scale industrialized production.
Summary of the invention
The present invention will solve the defective of above-mentioned prior art, and a kind of culture media composition and method thereof of suitable blueberry rooting of vitro seedling is provided.
The present invention solves the technical scheme that its technical problem adopts.The culture media composition of this suitable blueberry rooting of vitro seedling, the preparation of root media and every liter of contained weight are:
(1) minimal medium: 1/2WPM medium, white sugar 20g/L wherein, pH5.2～5.4;
(2) culture medium supports: perlite, 150～200g/L;
(3) root media: 1/2WPM+IBA0.03～0.1mg/L.
The tissue culture method of this suitable blueberry rooting of vitro seedling of the present invention, this method is carried out according to the following steps:
1), the preparation of root media and every liter of contained weight are:
(1) minimal medium: 1/2WPM medium, white sugar 20g/L wherein, pH5.2～5.4;
(2) culture medium supports: perlite, 150～200g/L;
(3) root media: 1/2WPM+IBA0.03～0.1mg/L;
2), the individual plant that will reach on 3-5 centimetre in the growth that proliferated culture medium was cultivated 40-50 days is inoculated on the root media, after 20～25 days, the seedling base portion grows several root systems;
3), the domestication and the transplanting of tissue cultivating seedling: with step 2) growth reaches the tissue cultivating seedling of taking root more than 3～7 centimetres, need not wash down root medium direct transplanting to cultivation matrix.
The condition of culture in described culture of rootage stage is, cultivation temperature is 25 ± 2 ℃, and the illumination light intensity is 1500～2500Lx, and light application time is 12h/d.
Described cultivation matrix is by peat: perlite 1: 1 by volume is formulated.
The invention has the beneficial effects as follows:
1), the blueberry that proposes takes root and optimizes that medium is with strong points, applicability good, owing to utilized perlite to be culture medium supports, thereby makes rooting rate reach 100%; Rootage duration foreshortens to 22-25 days, and transplanting survival rate is more than 90%.
2), this method has been owing to utilized perlite to be culture medium supports, culture medium supports can reuse, and need not clean when transplanting and save manually, reduces the damage to root system simultaneously, thereby effectively reduces group training cost, improves transplanting survival rate.
3), this method has been owing to utilized perlite to be culture medium supports, can direct transplanting after uncapping in cultivation matrix, thereby shortened adaptation time, increase work efficiency.
4) utilize running water to replace distilled water, white sugar place of sucrose among the present invention, thus saving group training cost.
Embodiment
The present invention is described in further detail by following examples, but content of the present invention is not limited thereto.
Embodiment 1: the culture media composition of this suitable blueberry rooting of vitro seedling in the present embodiment, and the preparation of root media and every liter of contained weight are:
(1) minimal medium: 1/2WPM medium, white sugar 20g/L wherein, pH5.2;
(2) culture medium supports: perlite, 150g/L;
(3) root media: 1/2WPM+IBA0.03mg/L.
The culture media composition tissue culture method of taking root in this suitable blueberry test-tube plantlet bottle in the present embodiment, carry out as follows:
1), the preparation of root media and every liter of contained weight are:
(1) minimal medium: 1/2WPM medium, white sugar 20g/L wherein, pH5.2;
(2) culture medium supports: perlite, 150g/L;
(3) root media: 1/2WPM+IBA0.03mg/L;
2), the individual plant that will reach on 3-5 centimetre in the growth that proliferated culture medium was cultivated 40-50 days is inoculated on the root media, after 20～25 days, the seedling base portion grows several root systems;
3), the domestication and the transplanting of tissue cultivating seedling: with step 2) growth reaches the tissue cultivating seedling of taking root more than 3～7 centimetres, need not wash down root medium direct transplanting to cultivation matrix.
The condition of culture in described culture of rootage stage is, cultivation temperature is 25 ± 2 ℃, and the illumination light intensity is 1500～2500Lx, and light application time is 12h/d.
Described cultivation matrix is by peat: perlite 1: 1 by volume is formulated.
Respectively organizing the condition of culture in training stage among this embodiment is: cultivation temperature is 25 ± 2 ℃, and the illumination light intensity is 1500～2500Lx, and light application time is 12h/d.
Embodiment 2: the culture media composition of this suitable blueberry rooting of vitro seedling in the present embodiment, and the preparation of root media and every liter of contained weight are:
(1) minimal medium: 1/2WPM medium, white sugar 20g/L wherein, pH5.4;
(2) culture medium supports: perlite, 200g/L;
(3) root media: 1/2WPM+IBA0.1mg/L.
The tissue culture method of the culture media composition of taking root in this suitable blueberry test-tube plantlet bottle in the present embodiment, carry out as follows:
1), the preparation of root media and every liter of contained weight are:
(1) minimal medium: 1/2WPM medium, white sugar 20g/L wherein, pH5.4;
(2) culture medium supports: perlite, 200g/L;
(3) root media: 1/2WPM+IBA0.1mg/L;
2), the individual plant that will reach on 3-5 centimetre in the growth that proliferated culture medium was cultivated 40-50 days is inoculated on the root media, after 20～25 days, the seedling base portion grows several root systems;
3), the domestication and the transplanting of tissue cultivating seedling: with step 2) growth reaches the tissue cultivating seedling of taking root more than 3～7 centimetres, need not wash down root medium direct transplanting to cultivation matrix.
The condition of culture in described culture of rootage stage is, cultivation temperature is 25 ± 2 ℃, and the illumination light intensity is 1500～2500Lx, and light application time is 12h/d.
Described cultivation matrix is by peat: perlite 1: 1 by volume is formulated.
Respectively organizing the condition of culture in training stage among this embodiment is: cultivation temperature is 25 ± 2 ℃, and the illumination light intensity is 1500～2500Lx, and light application time is 12h/d.
In addition to the implementation, the present invention can also have other embodiments.All employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop on the protection domain of requirement of the present invention.
Claims (4)
1. the culture media composition of a suitable blueberry rooting of vitro seedling, it is characterized in that: the preparation of root media and every liter of contained weight are:
(1) minimal medium: 1/2WPM medium, white sugar 20g/L wherein, pH5.2～5.4;
(2) culture medium supports: perlite, 150～200g/L;
(3) root media: 1/2WPM+IBA0.03～0.1mg/L.
2. the tissue culture method of a suitable blueberry rooting of vitro seedling, it is characterized in that: this method is carried out according to the following steps:
1), the preparation of root media and every liter of contained weight are:
(1) minimal medium: 1/2WPM medium, white sugar 20g/L wherein, pH5.2～5.4;
(2) culture medium supports: perlite, 150～200g/L;
(3) root media: 1/2WPM+IBA 0.03～0.1mg/L;
2), the individual plant that will reach on 3-5 centimetre in the growth that proliferated culture medium was cultivated 40-50 days is inoculated on the root media, after 20～25 days, the seedling base portion grows several root systems;
3), the domestication and the transplanting of tissue cultivating seedling: with step 2) growth reaches the tissue cultivating seedling of taking root more than 3～7 centimetres, need not wash down root medium direct transplanting to cultivation matrix.
3. the tissue culture method of suitable blueberry rooting of vitro seedling according to claim 2 is characterized in that: the condition of culture in described culture of rootage stage is, cultivation temperature is 25 ± 2 ℃, and the illumination light intensity is 1500～2500Lx, and light application time is 12h/d.
4. the tissue culture method of suitable blueberry rooting of vitro seedling according to claim 2 is characterized in that: described cultivation matrix is by peat: perlite 1: 1 by volume is formulated.
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| CNA2007101571946A CN101176429A (en) | 2007-11-28 | 2007-11-28 | Culture medium composition adapting for blueberry test tube plantlet radication as well as method thereof |
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Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101305693B (en) * | 2008-07-08 | 2010-11-03 | 山东省果树研究所 | High irrigation blueberry cultured shoots one-step seedling establishment method |
| CN101356888B (en) * | 2008-09-12 | 2010-11-10 | 大连大学 | Highbush blueberries winter-proofing method |
| CN102318559A (en) * | 2011-08-19 | 2012-01-18 | 温州市农业科学研究院 | Culture medium composition for rapid rooting of blueberry tissue culture seedling outside test tube and preparation method |
| CN102461462A (en) * | 2010-11-04 | 2012-05-23 | 湖北正佳微生物工程股份有限公司 | Preparation method of culture medium suitable for rapid seedling growing tissue culture of blueberry |
| CN102577967A (en) * | 2012-03-02 | 2012-07-18 | 湖州师范学院 | High-efficiency rapid rabbit-eye blueberry breeding method through tissue culture |
| CN102823499A (en) * | 2012-09-20 | 2012-12-19 | 重庆文理学院 | Factorized breeding method of blueberry tissue-cultured seedlings |
| CN103404434A (en) * | 2013-08-05 | 2013-11-27 | 浙江省农业科学院 | Prevention technology for multiple blueberry viruses at early stage |
| CN103718938A (en) * | 2013-12-27 | 2014-04-16 | 孟祖钢 | Rooting method using system with upper liquid draining holes, lower liquid draining holes and pressure boosting device |
| CN103875515A (en) * | 2014-02-18 | 2014-06-25 | 杭州佑国生物科技有限公司 | Ex-vitro rooting method for tissue culture blueberry seedlings |
| CN104137776A (en) * | 2014-07-24 | 2014-11-12 | 陕西理工学院 | Blueberry tissue cultured seedling liquid rooting method and special culturing container |
| CN105210874A (en) * | 2015-10-15 | 2016-01-06 | 铜仁市金农绿色农业科技有限公司 | A kind of blueberry seedling-cultivating method |
| CN105660330A (en) * | 2016-01-19 | 2016-06-15 | 黄庆辉 | Culture medium for raising germination rate of ginseng seeds |
| CN105900843A (en) * | 2016-05-10 | 2016-08-31 | 中国科学院植物研究所 | Bog bilberry tissue culture fast breeding method and culture medium thereof |
| CN106613962A (en) * | 2016-11-14 | 2017-05-10 | 宁波市农业科学研究院 | Culture medium for rooting of tissue-cultured blueberry seedling and culturing method using same |
| CN108651276A (en) * | 2017-03-29 | 2018-10-16 | 北京林业大学 | Rapid Rooting cultural method in blueberry tissue bag |
| CN114946625A (en) * | 2022-06-09 | 2022-08-30 | 安徽科技学院 | Method for rooting outside test tube of pomegranate tissue culture seedling |
-
2007
- 2007-11-28 CN CNA2007101571946A patent/CN101176429A/en active Pending
Cited By (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101305693B (en) * | 2008-07-08 | 2010-11-03 | 山东省果树研究所 | High irrigation blueberry cultured shoots one-step seedling establishment method |
| CN101356888B (en) * | 2008-09-12 | 2010-11-10 | 大连大学 | Highbush blueberries winter-proofing method |
| CN102461462A (en) * | 2010-11-04 | 2012-05-23 | 湖北正佳微生物工程股份有限公司 | Preparation method of culture medium suitable for rapid seedling growing tissue culture of blueberry |
| CN102318559A (en) * | 2011-08-19 | 2012-01-18 | 温州市农业科学研究院 | Culture medium composition for rapid rooting of blueberry tissue culture seedling outside test tube and preparation method |
| CN102577967A (en) * | 2012-03-02 | 2012-07-18 | 湖州师范学院 | High-efficiency rapid rabbit-eye blueberry breeding method through tissue culture |
| CN102823499A (en) * | 2012-09-20 | 2012-12-19 | 重庆文理学院 | Factorized breeding method of blueberry tissue-cultured seedlings |
| CN103404434A (en) * | 2013-08-05 | 2013-11-27 | 浙江省农业科学院 | Prevention technology for multiple blueberry viruses at early stage |
| CN103404434B (en) * | 2013-08-05 | 2015-05-06 | 浙江省农业科学院 | Prevention technology for multiple blueberry viruses at early stage |
| CN103718938B (en) * | 2013-12-27 | 2015-03-11 | 孟祖钢 | Rooting method using system with upper liquid draining holes, lower liquid draining holes and pressure boosting device |
| CN103718938A (en) * | 2013-12-27 | 2014-04-16 | 孟祖钢 | Rooting method using system with upper liquid draining holes, lower liquid draining holes and pressure boosting device |
| CN103875515A (en) * | 2014-02-18 | 2014-06-25 | 杭州佑国生物科技有限公司 | Ex-vitro rooting method for tissue culture blueberry seedlings |
| CN103875515B (en) * | 2014-02-18 | 2017-01-04 | 杭州佑国生物科技有限公司 | A kind of blueberry tissue culture outside sprout-cultivating-bottle radication method |
| CN104137776A (en) * | 2014-07-24 | 2014-11-12 | 陕西理工学院 | Blueberry tissue cultured seedling liquid rooting method and special culturing container |
| CN105210874A (en) * | 2015-10-15 | 2016-01-06 | 铜仁市金农绿色农业科技有限公司 | A kind of blueberry seedling-cultivating method |
| CN105660330A (en) * | 2016-01-19 | 2016-06-15 | 黄庆辉 | Culture medium for raising germination rate of ginseng seeds |
| CN105900843A (en) * | 2016-05-10 | 2016-08-31 | 中国科学院植物研究所 | Bog bilberry tissue culture fast breeding method and culture medium thereof |
| CN105900843B (en) * | 2016-05-10 | 2018-01-26 | 中国科学院植物研究所 | A kind of method and its culture medium of great bilberry tissue-culturing rapid propagation |
| CN106613962A (en) * | 2016-11-14 | 2017-05-10 | 宁波市农业科学研究院 | Culture medium for rooting of tissue-cultured blueberry seedling and culturing method using same |
| CN108651276A (en) * | 2017-03-29 | 2018-10-16 | 北京林业大学 | Rapid Rooting cultural method in blueberry tissue bag |
| CN114946625A (en) * | 2022-06-09 | 2022-08-30 | 安徽科技学院 | Method for rooting outside test tube of pomegranate tissue culture seedling |
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Open date: 20080514 |