CN1256864C - Poria attraction cultivating method - Google Patents
Poria attraction cultivating method Download PDFInfo
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- CN1256864C CN1256864C CN 03128016 CN03128016A CN1256864C CN 1256864 C CN1256864 C CN 1256864C CN 03128016 CN03128016 CN 03128016 CN 03128016 A CN03128016 A CN 03128016A CN 1256864 C CN1256864 C CN 1256864C
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- sclerotium
- poria cocos
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- pine
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Abstract
The present invention provides a poria attraction cultivating method which relates to a manual method for poria cultivation. The method comprises the following steps that pine materials are used as cultivation materials on which a pure poria hypha strain which is separated and cultivated manually is inoculated so that the poria hypha in the strain enters the pine materials of which the nutrition is decomposed and converted, the growth is enlarged, and a hypha body is formed. When the hypha body grows and develops the degree close to aggregation and kink, a small fresh young poria sclerotium block with vitality in the same type with that of an original poria inoculating strain is selected and is planted on the cultivation materials; the hypha body is attracted to the position for the positioning, the aggregation and the kink, and a new generation of sclerotium rudiment is formed; furthermore, the sclerotium with a larger individual is formed and developed. The present invention can accelerate the aggregation and the kink of the hypha body so that a sclerotium rudiment stage is completed ahead of schedule, the generated sclerotium has the advantages of large body, high yield, good quality and few pine materials correspondingly used; the yield quantity of processed poria pieces and blocks is large.
Description
Technical field the present invention relates to a kind of method of artificial cultivation Poria cocos.
The background technology Poria cocos is China's tradition conventional Chinese medicine material, is widely used in tcm clinical practice prescription, Chinese patent drug and healthy food material.
Basic former (the former plant) of Poria cocos medicinal material is Polyporaceae fungi Poria cocos Poria cocos (Schw.) Wolf.Its medicinal part is its sclerotium, and it forms medicinal material through peeling, cutting, drying.
The forming process of poria cocos sclerotium is: host's's (composts or fertilisers of cultivating) nutrition is decomposed, transformed to the Poria cocos mycelia under the adapt circumstance condition, propagation forms mycelium.When mycelial growth arrives certain scale (quantity), to grow to the regular period, gathering, knot form the mycelium agglomerate, and then form the sclerotium blank.After the sclerotium blank produces, Poria mycelium therebetween will decompose, transform the nutriment on host's (composts or fertilisers of cultivating), be transported to continuously in the sclerotium blank, accumulate, preserve, and make it to increase gradually, grow, and maturation is a sclerotium.
Storage has the Poria cocos mycelia of a large number of nutrients and some in the ripe poria cocos sclerotium.Under certain condition, the mycelia in the sclerotium can recover vitality, and then decomposes, transforms host's nutrition, forms mycelium, further produces sclerotium, so fresh sclerotium can be made kind of a usefulness.
Ancient times, the method that people plant Poria cocos is to make composts or fertilisers of cultivating with pine, plant with fresh sclerotium, under the artificial condition of creating, impel the Poria cocos mycelia in the sclerotium to recover vitality, enter pine, decompose, transform pine nutrition, enlarge growth, form mycelium, and then grow the formation sclerotium, this method is called " meat draws " cultivation.The seventies, scientific worker's using microbe separate tissue technology is isolated the Poria cocos mycelia from bright poria cocos sclerotium, through screening, expansion, cultivate the Poria cocos pure hypha bacterial, and replace tradition " meat draws " (bright sclerotium) to make kind of a plantation Poria cocos, be called " bacterial classification " cultivation with it.
For a long time,, also be to use " bacterial classification " cultivation Poria cocos, all only noted Poria cocos mycelia surviving and extent of growth in composts or fertilisers of cultivating (pine) no matter be to use " meat draws ", not enough to concern, the research of sclerotium formation and growth.As " with the method for living pine parasitic cultivation Poria cocos " (number of patent application 93102785), be exactly that the Poria cocos bacterial classification is planted cultivation, original seed enlarged culture, cultivated species enlarged culture through purification and rejuvenation, mother, under the living pine root, cultivate then.Their ubiquity mycelial growths are good, and forming sclerotium does not have fixing point, and many, individual little, the most growths of its quantity are relatively poor, have in addition excessive growth, do not tie problems such as sclerotium.Yield rates such as the sheet that individual little sclerotium cutting goes out, piece are low, and by product (fuling peel, broken Poria cocos etc.) is many, thereby the output value is not high.
Summary of the invention the objective of the invention is at above-mentioned present situation, aims to provide a kind of energy and quickens mycelial gathering, knot, impels the sclerotium blank stage to fulfil ahead of schedule, and the Poria cocos that the sclerote of generation is big lures and draws cultivation method.
The implementation of the object of the invention is that Poria cocos lures and draws cultivation method, makes composts or fertilisers of cultivating with pine, inoculate thereon from bright poria cocos sclerotium and separate, cultivate the Poria cocos pure hypha bacterial, make the Poria cocos mycelia in the bacterial classification enter pine, decompose, transform pine nutrition, enlarge growth, form mycelium.When mycelial growth is grown when closing on gathering, knot, select for use with the same strain of former inoculation poria cocos sclerotium fresh, have a tender little poria cocos sclerotium piece of blodynamic children, be implanted on the composts or fertilisers of cultivating, mycelium on the composts or fertilisers of cultivating lured guide to this and position gathering, knot, generate sclerotium blank of new generation, develop into individual bigger new sclerotium again.
The present invention grows when closing on gathering, knot at mycelial growth, generally be behind the cultivating bacterial spawn about 20 days, Poria mycelium by the composts or fertilisers of cultivating apical growth, extend to the other end, outward appearance is even, vigorous, as seen pencil shoestring, during cleaning-less bacteria infection, implant one fresh, have a tender little poria cocos sclerotium piece of blodynamic children, the weight of the tender little poria cocos sclerotium piece of children is 100 ± 10g.Because between the Poria mycelium of different strains antagonism is arranged, therefore, the poria cocos sclerotium piece of implantation should belong to same strain with the poria cocos sclerotium of cultivating bacterial spawn, otherwise, the mycelium of implanting can not merge with the mycelium in the composts or fertilisers of cultivating, and is withered gradually withered, loses effectiveness.
After implanting the tender little poria cocos sclerotium piece of children, the sclerotium piece plays inducing action to the mycelium in the composts or fertilisers of cultivating, the mycelium that has quickened in the composts or fertilisers of cultivating positions gathering, knot around this, impel the sclerotium blank stage to fulfil ahead of schedule, and enter normal growth, the stage of ripeness, compare with traditional cultivation method, Poria cocos knot Siberian cocklebur (sclerotium maturation) time of the present invention will shorten 1 month.
Adopt inductive technology of the present invention, make mycelium gathering, knot around the poria cocos sclerotium piece of implanting in the composts or fertilisers of cultivating, solved that the sclerotium that adopts traditional cultivating bacterial spawn often to occur does not have fixing point, quantity is many, individuality is little, of poor quality, or only long mycelia do not tie the hohlraum phenomenon of Siberian cocklebur (sclerotium), and output is significantly increased.
Adopt inductive technology of the present invention, on composts or fertilisers of cultivating, implant a young tender sclerotium piece, make the interior mycelium of composts or fertilisers of cultivating at this gathering, knot, form a sclerotium, so newly-generated sclerotium individuality is bigger, medicinal material finished product quantity such as the Poria cocos sheet that processes, Poria cocos piece are many, and by product (fuling peel, broken Poria cocos etc.) is few, increase the output value, improved the finished product rate.Mycelium is being implanted the quick fusion of sclerotium, expanding propagation on every side, and generates sclerotium, has quickened the gathering of nutriment in the sclerotium, and the inherent quality of product is effectively improved.
Owing to adopt the present invention to improve the output and the quality of Poria cocos, can reduce consumption accordingly as the pine of composts or fertilisers of cultivating.
The present invention can quicken mycelial gathering, knot, impels the sclerotium blank stage to fulfil ahead of schedule, and newly-generated sclerote is big, output is high, quality is good, few with pine relatively.Poria cocos sheet, the Poria cocos piece finished product quantity of processing are many.
Embodiment
The present invention makes composts or fertilisers of cultivating with pine, inoculates the Poria cocos pure hypha bacterial that separates, cultivates from bright poria cocos sclerotium thereon, makes the Poria cocos mycelia in the Poria cocos bacterial classification enter pine, decomposes, transforms pine nutrition, enlarges growth, forms mycelium.When mycelial growth is grown when closing on gathering, knot, select for use with the same strain of former inoculation poria cocos sclerotium fresh, have a tender little poria cocos sclerotium piece of blodynamic children, be implanted on the composts or fertilisers of cultivating, mycelium on the composts or fertilisers of cultivating lured guide to this and position gathering, knot, generate sclerotium blank of new generation, grow the individual bigger new sclerotium of formation again.The weight of the young tender little poria cocos sclerotium piece of implanting is 100 ± 10g.The present invention can quicken mycelial gathering, knot, impels the sclerotium blank stage to fulfil ahead of schedule, and newly-generated sclerote is big, output is high, quality is good, few with pine relatively.Poria cocos sheet, the Poria cocos piece finished product quantity of processing are many.
Claims (2)
1, Poria cocos lures and draws cultivation method, make composts or fertilisers of cultivating with pine, inoculate thereon from bright poria cocos sclerotium and separate, the Poria cocos pure hypha bacterial of cultivating, make the Poria cocos mycelia in the bacterial classification enter pine, decompose, transform pine nutrition, enlarge growth, form mycelium, it is characterized in that growing and closing on gathering when mycelial growth, during knot, select fresh with the same strain of former inoculation poria cocos sclerotium for use, have the tender little poria cocos sclerotium piece of blodynamic children, be implanted on the composts or fertilisers of cultivating, the mycelium on the composts or fertilisers of cultivating is lured guide to this and position gathering, knot, generate sclerotium blank of new generation, grow the individual bigger new sclerotium of formation again.
2, Poria cocos according to claim 1 lures and draws cultivation method, it is characterized in that the weight of the tender little poria cocos sclerotium piece of children is 100 ± 10g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03128016 CN1256864C (en) | 2003-05-23 | 2003-05-23 | Poria attraction cultivating method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03128016 CN1256864C (en) | 2003-05-23 | 2003-05-23 | Poria attraction cultivating method |
Publications (2)
| Publication Number | Publication Date |
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| CN1451262A CN1451262A (en) | 2003-10-29 |
| CN1256864C true CN1256864C (en) | 2006-05-24 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 03128016 Expired - Fee Related CN1256864C (en) | 2003-05-23 | 2003-05-23 | Poria attraction cultivating method |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103210786A (en) * | 2012-09-27 | 2013-07-24 | 安徽金寨乔康药业有限公司 | Method for planting tuckahoe by mother tuckahoe fixed-point core forming |
| CN102934586B (en) * | 2012-11-22 | 2014-03-19 | 福建省农业科学院食用菌研究所 | Low-carbon and high-yield poria culture method |
| CN102939858A (en) * | 2012-11-23 | 2013-02-27 | 贵州森泰实业有限公司 | Poria cocos compound cultivating method |
| CN104221715A (en) * | 2014-09-23 | 2014-12-24 | 叶成伍 | Poria cocos inducing cultivation method |
| CN104350920A (en) * | 2014-10-31 | 2015-02-18 | 颍上县永祥旱粮研究所 | Method for interplanting sesame among poria cocos |
| CN114503875B (en) * | 2022-01-30 | 2023-03-17 | 陕西森盛菌业科技有限公司 | Directional growth cultivation method for poria cocos |
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2003
- 2003-05-23 CN CN 03128016 patent/CN1256864C/en not_active Expired - Fee Related
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