CN106577220A - Culture medium for cultivating dendrobium ottYcinal K imuraet M igo and manufacturing method thereof - Google Patents
Culture medium for cultivating dendrobium ottYcinal K imuraet M igo and manufacturing method thereof Download PDFInfo
- Publication number
- CN106577220A CN106577220A CN201611009030.4A CN201611009030A CN106577220A CN 106577220 A CN106577220 A CN 106577220A CN 201611009030 A CN201611009030 A CN 201611009030A CN 106577220 A CN106577220 A CN 106577220A
- Authority
- CN
- China
- Prior art keywords
- product
- parts
- culture medium
- dendrobium candidum
- safflower
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Preparation Of Fruits And Vegetables (AREA)
Abstract
The invention discloses a culture medium for cultivating dendrobium ottYcinal K imuraet M igo and a preparation method thereof. The culture medium is mainly composed of citric acid, peptone, potatoes, bananas, agar, radix ophiopogonis, schisandra chinensis, safflower and radix paeoniae rubra. When the dendrobium ottYcinal K imuraet M igo is cultivated with the culture medium disclosed by the invention, tissue culture seedlings can grow well, and the growth value and the proliferation rate are high.
Description
Technical field
The present invention relates to a kind of culture medium for cultivating dendrobium candidum and preparation method thereof, particularly a kind of plantlet in vitro life
Dendrobium officinale culture medium grown and preparation method thereof.
Background technology
Orchid (Orchidaceae) has more than 750 category more than 20000 to plant, except the flower and leaf of numerous species have
Outside higher ornamental value, if some species such as dendrobium have medical value, the stem of noble dendrobium be divided into iron sheet, yellow grass, Jin Chai, ring grass,
Several kinds of Huoshan, horsewhip etc., wherein dendrobium candidum (Dendrobium ottYcinal K imuraet M igo) are the most precious.
Dendrobium candidum, also known as bracketplant, iron sheet orchid, iron sheet bracketplant, Yunnan iron sheet, is orchid family Dendrobium herbaceos perennial, and it is the stem of noble dendrobium
The top grade of class medicinal material, is used as medicine with its stem, containing polysaccharide, dendrobine, the trace element of various needed by human body and amino acid etc. effectively
Composition, in antitumor, raising body immunity, treatment enterogastric diseases, anti-aging, anti-oxidant, platelet aggregation-against, reduction blood
The aspect such as sugar and treatment cataract has good efficacy.
At present, dendrobium candidum is achieved in preserving seed and quick proliferation achieved with certain using tissue culture technology, but
Improved seeds Jing subcultures or because of factors such as Corticosteroids are improper, culture environments for several times, plantlet in vitro growth fraction is slower, the rate of increase
It is low.
The content of the invention
The purpose of the present invention, is to provide a kind of culture medium for cultivating dendrobium candidum and preparation method thereof, with the present invention
During medium culture dendrobium candidum, plantlet in vitro grows, and growth value and the rate of increase are high, and hormone is not contained in culture medium.
What the present invention was realized in.
A kind of culture medium for cultivating dendrobium candidum, calculates by weight, mainly by citric acid 1-10 parts, peptone
10-20 parts, potato 50-60 parts, banana 50-60 parts, agar 20-30 parts, tuber of dwarf lilyturf 5-15 part, fruit of Chinese magnoliavine 10-20 parts, safflower 1-
10 parts are made with radix paeoniae rubrathe 1-10 parts.
It is previously described for cultivating the culture medium of dendrobium candidum, calculates by weight, mainly by citric acid 3-7 parts, peptone
13-17 parts, potato 53-57 parts, banana 53-57 parts, agar 23-27 parts, tuber of dwarf lilyturf 8-12 part, fruit of Chinese magnoliavine 13-17 parts, safflower 3-
7 parts are made with radix paeoniae rubrathe 3-7 parts.
It is previously described for cultivating the culture medium of dendrobium candidum, calculates by weight, mainly by 5 parts of citric acid, peptone 15
5 parts of part, 55 parts of potato, 55 parts of banana, 25 parts of agar, 10 parts of the tuber of dwarf lilyturf, 15 parts of the fruit of Chinese magnoliavine, 5 parts of safflower and the radix paeoniae rubrathe are made.
The preparation method of the culture medium of cultivation dendrobium candidum is previously described for, is comprised the steps：
(1) tuber of dwarf lilyturf, the fruit of Chinese magnoliavine, safflower and the radix paeoniae rubrathe are taken, after being ground into fine grained, plus 8-12 times is measured decocting and boil 2-3h, is collected
Decoction liquor, obtains A product；
(2) citric acid and peptone are added at room temperature in A product, stirs complete to dissolving, obtain B product；
(3) potato is added water after boiling, rubs into mashed potato, obtain C product；
(4) C product are added in B product, is stirred, obtain D product；
(5) add in D product after banana is blended, stir, obtain E product；
(6) agar adds at room temperature 3-5 times and measures water, stirs to after being completely dissolved, and obtains F product；
(7) F product are added in E product, are mixed, and obtain G product；
(8) G product high pressure steam sterilization 20-30 minutes, are obtained final product loaded in tissue culture bottle after cooled and solidified.
It is previously described for the preparation method of the culture medium of cultivation dendrobium candidum：Step (1) is：Take the tuber of dwarf lilyturf, the fruit of Chinese magnoliavine, safflower
And the radix paeoniae rubrathe, after being ground into fine grained, plus 10 times of amount decoctings boil 2.5h, collect decoction liquor, obtain A product.
It is previously described for the preparation method of the culture medium of cultivation dendrobium candidum：Step (6) is：Agar adds at room temperature 4 times
Amount water, stirs to after being completely dissolved, and obtains F product.
It is previously described for the preparation method of the culture medium of cultivation dendrobium candidum：Step (8) is：G product are high loaded in tissue culture bottle
Pressure steam sterilizing 20-30 minutes, obtain final product after cooled and solidified.
Applicant has carried out substantial amounts of experimental study to the present invention, and part Experiment is as follows：
Experimental example.
1 materials and methods
1.1 material
1.1.1 the kinds of dendrobium candidum T 4 (original seed is picked up from county of Guangnan of Yunnan) plantlet in vitro.
1.1.2 culture medium of the present invention：Made by embodiment 1；
1.1.3 culture medium is contrasted：Raw material does not contain the tuber of dwarf lilyturf, the fruit of Chinese magnoliavine, safflower and the radix paeoniae rubrathe, and remaining preparation method is with embodiment 1.
1.2 method
1.2.1 culture experiment
The plantlet in vitro of slight chrysanthemum distortion is taken, after removing root pocket, 6 bottles of each culture medium inoculated, per bottle is inoculated with 15 plants, 50d
After observe, count plantlet in vitro growing state.
1.2.2 condition of culture
The culture of each test is in day temperature (22 scholar 1) DEG C, nocturnal temperature (18 ± 1) DEG C, illumination 1800Lx, illumination
Between cultivate in 14h/d and draughty tissue culture culturing room.
1.2.3 statistical method
Average plant height during average plant height-subculture during growth value (cm)=statistics；
The rate of increase (%)=(seedling sum during one subculture of seedling sum during statistics)/initial seedling sum × 100%.
3 results
The impact of 3.1 pairs of candidum tissue culturing seedling growths
Growing state Jing after 50d cultures is shown in Table 1.
The impact that table 1 grows to plantlet in vitro
| Culture medium | Growth value/cm | The rate of increase/% |
| Contrast culture medium | 1.40 | 225.6 |
| Culture medium of the present invention | 1.75 | 295.5 |
As seen from table, culture medium group growth value of the present invention and appreciation rate are above contrasting culture medium group.
When compared with prior art, with medium culture dendrobium candidum of the present invention, plantlet in vitro grows, growth value and propagation
Rate is high.
Specific embodiment
Embodiment 1.
Formula：Citric acid 50kg, peptone 150kg, potato 550kg, banana 550kg, agar 250kg, the tuber of dwarf lilyturf
100kg, fruit of Chinese magnoliavine 150kg, safflower 50kg and radix paeoniae rubrathe 50kg.
Manufacture craft：
(1) tuber of dwarf lilyturf, the fruit of Chinese magnoliavine, safflower and the radix paeoniae rubrathe are taken, after being ground into fine grained, plus 10 times of amount decoctings boil 2.5h, collect and decoct
Boil liquid, obtains A product；
(2) citric acid and peptone are added at room temperature in A product, stirs complete to dissolving, obtain B product；
(3) potato is added water after boiling, rubs into mashed potato, obtain C product；
(4) C product are added in B product, is stirred, obtain D product；
(5) add in D product after banana is blended, stir, obtain E product；
(6) agar adds at room temperature 4 times of amount water, stirs to after being completely dissolved, and obtains F product；
(7) F product are added in E product, are mixed, and obtain G product；
(8) loaded in tissue culture bottle, high pressure steam sterilization 25 minutes is obtained final product G product after cooled and solidified.
Embodiment 2.
Formula：Citric acid 100kg, peptone 200kg, potato 600kg, banana 600kg, agar 300kg, the tuber of dwarf lilyturf
150kg, fruit of Chinese magnoliavine 200kg, safflower 100kg and radix paeoniae rubrathe 100kg.
Manufacture craft：
(1) tuber of dwarf lilyturf, the fruit of Chinese magnoliavine, safflower and the radix paeoniae rubrathe are taken, after being ground into fine grained, plus 12 times of amount decoctings boil 3h, collect and decoct
Liquid, obtains A product；
(2) citric acid and peptone are added at room temperature in A product, stirs complete to dissolving, obtain B product；
(3) potato is added water after boiling, rubs into mashed potato, obtain C product；
(4) C product are added in B product, is stirred, obtain D product；
(5) add in D product after banana is blended, stir, obtain E product；
(6) agar adds at room temperature 5 times of amount water, stirs to after being completely dissolved, and obtains F product；
(7) F product are added in E product, are mixed, and obtain G product；
(8) loaded in tissue culture bottle, high pressure steam sterilization 30 minutes is obtained final product G product after cooled and solidified.
Embodiment 3.
Formula：Citric acid 10kg, peptone 100kg, potato 500kg, banana 500kg, agar 200kg, tuber of dwarf lilyturf 50kg,
Fruit of Chinese magnoliavine 100kg, safflower 10kg and radix paeoniae rubrathe 10kg.
Manufacture craft：
(1) tuber of dwarf lilyturf, the fruit of Chinese magnoliavine, safflower and the radix paeoniae rubrathe are taken, after being ground into fine grained, plus 8 times of amount decoctings boil 2h, collect and decoct
Liquid, obtains A product；
(2) citric acid and peptone are added at room temperature in A product, stirs complete to dissolving, obtain B product；
(3) potato is added water after boiling, rubs into mashed potato, obtain C product；
(4) C product are added in B product, is stirred, obtain D product；
(5) add in D product after banana is blended, stir, obtain E product；
(6) agar adds at room temperature 3 times of amount water, stirs to after being completely dissolved, and obtains F product；
(7) F product are added in E product, are mixed, and obtain G product；
(8) loaded in tissue culture bottle, high pressure steam sterilization 20 minutes is obtained final product G product after cooled and solidified.
Claims (7)
1. a kind of culture medium for cultivating dendrobium candidum, it is characterised in that：Calculate by weight, mainly by citric acid 1-10
Part, peptone 10-20 parts, potato 50-60 parts, banana 50-60 parts, agar 20-30 parts, tuber of dwarf lilyturf 5-15 part, fruit of Chinese magnoliavine 10-20
Part, safflower 1-10 parts and radix paeoniae rubrathe 1-10 parts are made.
2. it is used to as claimed in claim 1 cultivate the culture medium of dendrobium candidum, it is characterised in that：Calculate by weight, mainly
By citric acid 3-7 parts, peptone 13-17 parts, potato 53-57 parts, banana 53-57 parts, agar 23-27 parts, tuber of dwarf lilyturf 8-12 part,
Fruit of Chinese magnoliavine 13-17 parts, safflower 3-7 parts and radix paeoniae rubrathe 3-7 parts are made.
3. it is used to as claimed in claim 2 cultivate the culture medium of dendrobium candidum, it is characterised in that：Calculate by weight, mainly
By 5 parts of citric acid, 15 parts of peptone, 55 parts of potato, 55 parts of banana, 25 parts of agar, 10 parts of the tuber of dwarf lilyturf, 15 parts of the fruit of Chinese magnoliavine, safflower 5
5 parts of part and the radix paeoniae rubrathe are made.
4. the preparation method for cultivating the culture medium of dendrobium candidum as any one of claim 1-3, its feature exists
In：Comprise the steps：
（1）The tuber of dwarf lilyturf, the fruit of Chinese magnoliavine, safflower and the radix paeoniae rubrathe are taken, after being ground into fine grained, plus 8-12 times is measured decocting and boil 2-3h, is collected and is decocted
Liquid, obtains A product；
（2）Citric acid and peptone are added at room temperature in A product, stirs complete to dissolving, obtain B product；
（3）Potato is added water after boiling, rub into mashed potato, obtain C product；
（4）C product are added in B product, is stirred, obtain D product；
（5）Banana is added in D product after blending, and is stirred, and obtains E product；
（6）Agar adds at room temperature 3-5 times and measures water, stirs to after being completely dissolved, and obtains F product；
（7）F product are added in E product, are mixed, and obtain G product；
（8）G product high pressure steam sterilization 20-30 minutes, are obtained final product loaded in tissue culture bottle after cooled and solidified.
5. the preparation method of the as claimed in claim 4 culture medium for being used to cultivate dendrobium candidum, it is characterised in that：Step（1）
For：The tuber of dwarf lilyturf, the fruit of Chinese magnoliavine, safflower and the radix paeoniae rubrathe are taken, after being ground into fine grained, plus 10 times of amount decoctings boil 2.5h, collect decoction liquor, obtain A
Product.
6. the preparation method of the as claimed in claim 4 culture medium for being used to cultivate dendrobium candidum, it is characterised in that：Step（6）
For：Agar adds at room temperature 4 times of amount water, stirs to after being completely dissolved, and obtains F product.
7. the preparation method of the as claimed in claim 4 culture medium for being used to cultivate dendrobium candidum, it is characterised in that：Step（8）
For：G product high pressure steam sterilization 20-30 minutes, are obtained final product loaded in tissue culture bottle after cooled and solidified.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611009030.4A CN106577220A (en) | 2016-11-16 | 2016-11-16 | Culture medium for cultivating dendrobium ottYcinal K imuraet M igo and manufacturing method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611009030.4A CN106577220A (en) | 2016-11-16 | 2016-11-16 | Culture medium for cultivating dendrobium ottYcinal K imuraet M igo and manufacturing method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106577220A true CN106577220A (en) | 2017-04-26 |
Family
ID=58591103
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611009030.4A Pending CN106577220A (en) | 2016-11-16 | 2016-11-16 | Culture medium for cultivating dendrobium ottYcinal K imuraet M igo and manufacturing method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106577220A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107027606A (en) * | 2017-05-17 | 2017-08-11 | 贵州济生农业科技有限公司 | A kind of cultural method of the high dendrobium candidum of stem of noble dendrobium alkali content |
-
2016
- 2016-11-16 CN CN201611009030.4A patent/CN106577220A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107027606A (en) * | 2017-05-17 | 2017-08-11 | 贵州济生农业科技有限公司 | A kind of cultural method of the high dendrobium candidum of stem of noble dendrobium alkali content |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103416305B (en) | Hormone-free tissue culture and rapid propagation method of anoectochilus formosanus seedlings | |
| CN101455180B (en) | Open type plant tissue culture seedlings-raising method | |
| CN104041273B (en) | A kind of potted plant acclimation method of Chinese crude drug Folium Stevlae Rebaudianae | |
| CN104206137B (en) | The high-yield early-maturing of wild rice stem is transplanted Cultivate administration method | |
| CN102499088B (en) | Method for quickly breeding seedlings of Guangxi anoectochilus roxburghii capsules by utilizing Guangxi anoectochilus roxburghii capsules | |
| CN102884982A (en) | Hormone-free rapid propagation organic production method of anoectochilus formosanus germchit | |
| CN105104190A (en) | Anoectochilus roxburghii tissue culture seedling method | |
| CN107409662A (en) | A kind of alternating method for reducing the tobacco bacterial wilt incidence of disease | |
| CN106613832A (en) | Culture method of dendrobium officinale | |
| CN106613835A (en) | Cutting seedling-raising matrix for pomegranates | |
| CN108651195A (en) | A method of improving the growth of Rhizoma Atractylodis Macrocephalae seedling and drought-resistant ability | |
| CN107629967A (en) | Fluid nutrient medium and rhizoma Gastrodiae implantation methods for the Germination Strain of rhizoma Gastrodiae plantation | |
| KR101433209B1 (en) | Hydroponic Culture Media for Ginseng and Preparation Method Thereof | |
| CN105191803B (en) | A kind of candidum tissue culturing bag seedling production method | |
| CN107548992A (en) | A kind of imitating wild planting process of the good dendrobium candidum of growing way | |
| CN112119913A (en) | Virus-free propagation method for sweet potatoes | |
| CN106576769A (en) | Watermelon seedling cultivation method capable of resisting continuous cropping obstacles | |
| CN106577220A (en) | Culture medium for cultivating dendrobium ottYcinal K imuraet M igo and manufacturing method thereof | |
| CN105906443A (en) | Fertilizer special for non-polluted rice and preparation method thereof | |
| CN112931058B (en) | Separation and purification method of Moganshan wild Dictyophora rubrovalvata strain | |
| CN107821169A (en) | A kind of tissue culture method of P. kingianum seedling | |
| CN109566294B (en) | Method for planting wild dendrobium nobile in rock | |
| CN109874679B (en) | Tissue culture and rapid propagation technical seedling method for solidago decurrens | |
| CN106613964A (en) | Dendrobium officinale culture medium and making method thereof | |
| CN107455134A (en) | A kind of rhizoma atractylodis cuttage and seedling culture method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170426 |