CN104041273B - A kind of potted plant acclimation method of Chinese crude drug Folium Stevlae Rebaudianae - Google Patents
A kind of potted plant acclimation method of Chinese crude drug Folium Stevlae Rebaudianae Download PDFInfo
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- CN104041273B CN104041273B CN201410243957.9A CN201410243957A CN104041273B CN 104041273 B CN104041273 B CN 104041273B CN 201410243957 A CN201410243957 A CN 201410243957A CN 104041273 B CN104041273 B CN 104041273B
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Abstract
The invention discloses the potted plant acclimation method of a kind of Chinese crude drug Folium Stevlae Rebaudianae, go out the steps such as garden including introduction and acclimatization, tissue culture, potted plant management, factorial praluction, finished product.Folium Stevlae Rebaudianae is planted Chinese crude drug and is converted into potted plant flower by the potted plant acclimation method of Chinese crude drug Folium Stevlae Rebaudianae of the present invention well from ground, appreciates for people, and survival rate is high, and operation is simple, low cost, improves the economic worth of Folium Stevlae Rebaudianae, is suitable for large-scale promotion.
Description
Technical field
The present invention relates to the potted plant acclimation method of a kind of Chinese crude drug Folium Stevlae Rebaudianae, belong to field of plant growing technology.
Background technology
Folium Stevlae Rebaudianae is perennial herb, plant height sweet l-1.3 rice.The root tip is loose, 50-60 bar, long up to 25 centimetres.Stem is straight
Vertical, base portion tip lignifying, top is tender, close raw short down.Leaf, to raw or stem top alternate, drapes over one's shoulders profile, and there is a shallow sawtooth at edge, and two
Face is gone out by short down, vein three.Head inflorescence is little, involucre tubular, and phyllary 5-6 layer, limit is isometric；Holder is smooth, bald only；Flower
Crown base portion light violet magenta or white, top white.Achene is linear, the most flat, brown, has pappus.Florescence 7 JIUYUE, really phase 9-11
Month.The happiness of habit Folium Stevlae Rebaudianae grows in the environment of warm and moist, but is also resistant to the low temperature of-5 DEG C, and temperature is at 20-30 DEG C
Time optimum stem and leaf growth.Folium Stevlae Rebaudianae belongs to the short-day plant strong to photoperiod sensitivity, and critical day-length is 12 hours, so
Low latitudes cultivation is bloomed relatively early.Chasmogamous ovule, needs the time of 25-30 days could grow for mature seed, works as kind
After sub-maturation, pappus waves with the wind and propagates everywhere.Folium Stevlae Rebaudianae, as a kind of Chinese crude drug, uses seminal propagation, cottage propagation more, divides
The propagation methods such as tiller breeding, then plant in big Tanaka, are used for potted plant method still about by the domestication of Chinese crude drug Folium Stevlae Rebaudianae at present
Without report.
Summary of the invention
It is an object of the invention to provide the potted plant acclimation method of a kind of Chinese crude drug Folium Stevlae Rebaudianae, Folium Stevlae Rebaudianae is tamed into potted plant
View and admire for people.
For achieving the above object, the technical solution used in the present invention is as follows:
The potted plant acclimation method of a kind of Chinese crude drug Folium Stevlae Rebaudianae, comprises the following steps:
(1) introduction and acclimatization
Carrying out the selection of seedling many generations, or difference little in two places condition difference slightly exceeds the ground of plant subject range
District, by sowing continuously introducing a fine variety area, the plant selecting strong stress resistance carries out introducing a fine variety breeding；
(2) tissue culture
When cutting Folium Stevlae Rebaudianae medical flower material from greenhouse domestication bed, should select healthy and strong without disease pest maternal plant, take that children is tender, divide
The position that raw ability is strong, in order to growth；
Surface sterilizing should be carried out before inoculation: first rinsed for ten a few minutes with tap water, have should brushing away of mud；After rinsing well,
With the sterilization in alcohol-pickled 10-15 second of 70%；Then use aseptic water washing twice, then soak with the clarified bleaching solution of 10%
Sterilization in 20-30 minute, finally uses aseptic water washing 3-4 time, and the operation of above-mentioned surface sterilizing all should be at inoculating hood or superclean bench
Carry out etc. gnotobasis condition；
Through the material of surface sterilizing, with aseptic filter paper, water is sopped up；Desired area, usual several millis are cut again with dissecting knife
Meter great little, cultivates virus-free Seedling and then then should be inoculated on culture bottle by material with dissecting needle or gun-type tweezers below 1 millimeter
Cultivate；Instrument is finished and i.e. should dip in 95% ethanol, or sterilizes by flame disinfection method, it is to avoid instrument carries disease germs and causes intersection dirt
Dye；
(3) potted plant management
By cultured Folium Stevlae Rebaudianae Seedling move into equipped with in the flowerpot of substrate, the preparation method of described substrate is as follows: (1) take with
The raw material of lower weight portion: vegetable garden soil 10-15, mountain mud 22-28, coarse sand 5-10, fish scale slag 12-16, miscellaneous bone meal 8-16, coconut palm bran
14-18, modified stone powder 5-10, turfy soil 45-55；(2) above-mentioned raw materials is got ready, put in proportion with sludging equipment, wait above-mentioned former
After material stirs in machine, stack 25-30 days, fill basin after natural fermentation standby；The preparation side of wherein said modified stone powder
Method is as follows: (1), by weight 5-10:4-6:2-4 sand JISHI, pelelith, galapectite mix homogeneously, calcines 1-2h for 850-900 DEG C,
It is cooled to room temperature, pulverizes, cross 50-100 mesh sieve, stand-by；(2) take and be equivalent to the Sapium sebiferum (L.) Roxb. of stone powder weight 3-5% that step (1) prepares
Leaf, the Radix Phytolaccae of 2-3%, the Flos Rhododendri Mollis of 1.5-2.5%, the leaf of Fructus Persicae of 2.5-4.5%, the Herba Cymbopogonis Citrari mix homogeneously of 2-3%, boiling
Extracting 2 times, add 10-15 times amount water for the first time, second time adds 5-10 times amount water, merges decoction liquor, filters, is concentrated into original volume
1/4-1/3, be subsequently adding and be equivalent to the polyethylene glycol oxide of concentrated solution weight 15-20%, the polyacrylamide of 10-15%, 4-8%
Hydroxypropyl methyl cellulose, 3-5% xanthan gum, the sodium alginate of 4-6%, stir, by equal for the colloid solution obtained
Even be sprayed at step (1) prepare stone powder on, dry, be cooled to room temperature；
After transplanting by pressurized circulation pump by nutritional solution in droping irrigating pipe is input to flowerpot, weekly 1 the morning drip irrigation 20-30 divide
Clock, all 3 the morning drip irrigation 20-30 minute, remaining time uses clear water drip irrigation；The component content of described nutritional solution is: potassium nitrate
0.3-0.5 g/L, calcium nitrate 0.2-0.4g/L, magnesium sulfate 0.1-0.2g/L, ammonium phosphate 0.2-0.3g/L, potassium sulfate 0.25-
0.35g/L, potassium dihydrogen phosphate 0.1-0.2g/L, b diammonium disodium edta 0.01-0.02g/L, ferrous sulfate 0.06-0.08g/
L, boric acid 0.005-0.015g/L, manganese sulfate 0.015-0.025g/L, zinc sulfate 0.006-0.012g/L, copper sulfate 0.2-
0.3mg/L, ammonium molybdate 0.05-0.1mg/L；Nutritional solution accomplishes that nutrient tank wants lucifuge with joining and can not deposit；
(4) factorial praluction
Be placed in warmhouse booth growth by potted plant for Folium Stevlae Rebaudianae, by pinching, pinch, control water, cut root, control plant height,
Reach potted plant and view and admire purpose；
1. pinching: mainly extract the bud point most with apical dominance, make growing tips of the plant change, promote induction lateral bud,
Reach the purpose that plant is plentiful, downgrade；
2. pinch: refer to that easy spindling plant is too fast because of growth, promote plant nutrient to divide by wiping out a part of apical meristem
Dissipate supply；
3. controlling water: when growing vigorous, the plant that makes controlling the water yield artificial is in semiarid state, reaches plant short and small, rich
Full, side shoot prosperity；
4. cut root: cut off main root by artificial instrument, control growth；
(5) finished product goes out garden
The method have the benefit that
Folium Stevlae Rebaudianae is planted Chinese crude drug and is converted into potted plant by the potted plant acclimation method of Chinese crude drug Folium Stevlae Rebaudianae of the present invention well from ground
Flower, appreciates for people, and survival rate is high, and operation is simple, low cost, improves the economic worth of Folium Stevlae Rebaudianae, is suitable for scale
Change and promote.
Detailed description of the invention
The potted plant acclimation method of a kind of Chinese crude drug Folium Stevlae Rebaudianae, comprises the following steps:
(1) introduction and acclimatization
Carrying out the selection of seedling many generations, or difference little in two places condition difference slightly exceeds the ground of plant subject range
District, by sowing continuously introducing a fine variety area, the plant selecting strong stress resistance carries out introducing a fine variety breeding；
(2) tissue culture
When cutting Folium Stevlae Rebaudianae medical flower material from greenhouse domestication bed, should select healthy and strong without disease pest maternal plant, take that children is tender, divide
The position that raw ability is strong, in order to growth；
Surface sterilizing should be carried out before inoculation: first rinsed for ten a few minutes with tap water, have should brushing away of mud；After rinsing well,
With the sterilizations in alcohol-pickled 15 seconds of 70%；Then use aseptic water washing twice, then soak 25 points with the clarified bleaching solution of 10%
Clock sterilizes, and finally uses aseptic water washing 3 times, and the operation of above-mentioned surface sterilizing all should be in the gnotobasis such as inoculating hood or superclean bench
Condition is carried out；
Through the material of surface sterilizing, with aseptic filter paper, water is sopped up；Desired area, usual several millis are cut again with dissecting knife
Meter great little, cultivates virus-free Seedling and then then should be inoculated on culture bottle by material with dissecting needle or gun-type tweezers below 1 millimeter
Cultivate；Instrument is finished and i.e. should dip in 95% ethanol, or sterilizes by flame disinfection method, it is to avoid instrument carries disease germs and causes intersection dirt
Dye；
(3) potted plant management
By cultured Folium Stevlae Rebaudianae Seedling move into equipped with in the flowerpot of substrate, the preparation method of described substrate is as follows: (1) take with
The raw material of lower weight portion: vegetable garden soil 12, mountain mud 26, coarse sand 8, fish scale slag 14, miscellaneous bone meal 10, coconut palm bran 16, modified stone powder 7, the peat composed of rotten mosses
Soil 50；(2) above-mentioned raw materials is got ready, put in proportion with sludging equipment, after waiting above-mentioned raw materials to stir in machine, stack 30
My god, fill basin after natural fermentation standby；The preparation method of wherein said modified stone powder is as follows: (1) amasss by weight 8:5:3 sand
Stone, pelelith, galapectite mix homogeneously, 880 DEG C of calcining 1h, it is cooled to room temperature, pulverizes, cross 80 mesh sieves, stand-by；(2) take quite
In step (1) prepare the leaf of Sapium sebiferum (L.) Roxb. of stone powder weight 4%, the Radix Phytolaccae of 2.5%, the Flos Rhododendri Mollis of 2%, the leaf of Fructus Persicae of 3.5%, the perfume (or spice) of 2.5%
Rhizoma Imperatae mix homogeneously, boiling is extracted 2 times, adds 12 times amount water for the first time, and second time adds 8 times amount water, merges decoction liquor, mistake
Filter, is concentrated into the 1/3 of original volume, is subsequently adding and is equivalent to the polyethylene glycol oxide of concentrated solution weight 16%, the polyacrylamide of 12%
Amine, the hydroxypropyl methyl cellulose of 6%, 4% xanthan gum, the sodium alginate of 5%, stir, by equal for the colloid solution obtained
Even be sprayed at step (1) prepare stone powder on, dry, be cooled to room temperature；
After transplanting by pressurized circulation pump by nutritional solution in droping irrigating pipe is input to flowerpot, weekly 1 the morning drip irrigation 30 minutes,
The morning in week 3 drip irrigation 30 minutes, remaining time uses clear water drip irrigation；The component content of described nutritional solution is: potassium nitrate 0.35 g/
L, calcium nitrate 0.25g/L, magnesium sulfate 0.13g/L, ammonium phosphate 0.22g/L, potassium sulfate 0.31g/L, potassium dihydrogen phosphate 0.15g/L,
B diammonium disodium edta 0.013g/L, ferrous sulfate 0.07g/L, boric acid 0.01g/L, manganese sulfate 0.02g/L, zinc sulfate
0.008g/L, copper sulfate 0.24mg/L, ammonium molybdate 0.08mg/L；Nutritional solution accomplishes that nutrient tank is wanted with joining and can not deposit
Lucifuge；
(4) factorial praluction
Be placed in warmhouse booth growth by potted plant for Folium Stevlae Rebaudianae, by pinching, pinch, control water, cut root, control plant height,
Reach potted plant and view and admire purpose；
1. pinching: mainly extract the bud point most with apical dominance, make growing tips of the plant change, promote induction lateral bud,
Reach the purpose that plant is plentiful, downgrade；
2. pinch: refer to that easy spindling plant is too fast because of growth, promote plant nutrient to divide by wiping out a part of apical meristem
Dissipate supply；
3. controlling water: when growing vigorous, the plant that makes controlling the water yield artificial is in semiarid state, reaches plant short and small, rich
Full, side shoot prosperity；
4. cut root: cut off main root by artificial instrument, control growth；
(6) finished product goes out garden
Tame Folium Stevlae Rebaudianae, survival rate to 97% by above method, can cultivate and also allow people eyeable potted plant.
Claims (1)
1. the potted plant acclimation method of a Chinese crude drug Folium Stevlae Rebaudianae, it is characterised in that comprise the following steps:
(1) introduction and acclimatization carries out the selection of seedling many generations, or difference little in two places condition difference slightly adapts to model beyond plant
The area enclosed, by sowing continuously introducing a fine variety area, the plant selecting strong stress resistance carries out introducing a fine variety breeding；
(2), when tissue culture cuts Folium Stevlae Rebaudianae medical flower material from greenhouse domestication bed, should select healthy and strong without disease pest maternal plant,
Take the position that children is tender, meristematic capacity is strong, in order to growth；Surface sterilizing should be carried out: first rinse ten somewhat with tap water before inoculation
Clock, has should brushing away of mud；After rinsing well, with the sterilization in alcohol-pickled 10-15 second of 70%；Then aseptic water washing is used twice,
Soaking sterilization in 20-30 minute with the clarified bleaching solution of 10% again, finally use aseptic water washing 3-4 time, above-mentioned surface sterilizing operates
All should carry out at inoculating hood or these gnotobasis conditions of superclean bench；Through the material of surface sterilizing, will with aseptic filter paper
Water sops up；Cutting desired area, usual several mm in sizes with dissecting knife again, cultivating virus-free Seedling then should be below 1 millimeter, then
With dissecting needle or gun-type tweezers, material is inoculated on culture bottle cultivation；Instrument is finished and i.e. should dip in 95% ethanol, or uses
Flame disinfection method is sterilized, it is to avoid instrument carries disease germs and causes cross-contamination；
(3) cultured Folium Stevlae Rebaudianae Seedling is moved into equipped with in the flowerpot of substrate by potted plant management, and the preparation method of described substrate is such as
Under: (1) takes the raw material of following weight portion: vegetable garden soil 10-15, mountain mud 22-28, coarse sand 5-10, fish scale slag 12-16, miscellaneous bone meal 8-
16, coconut palm bran 14-18, modified stone powder 5-10, turfy soil 45-55；
(2) above-mentioned raw materials is got ready, put in proportion with sludging equipment, after waiting above-mentioned raw materials to stir in machine, stack 25-30
My god, fill basin after natural fermentation standby；
The preparation method of wherein said modified stone powder is as follows:
(1) by weight 5-10:4-6:2-4 sand JISHI, pelelith, galapectite mix homogeneously, 850-900 DEG C of calcining 1-2h is cold
But to room temperature, pulverize, cross 50-100 mesh sieve, stand-by；
(2) take be equivalent to step (1) prepare the leaf of Sapium sebiferum (L.) Roxb. of stone powder weight 3-5%, the Radix Phytolaccae of 2-3%, the Flos Rhododendri Mollis of 1.5-2.5%,
The leaf of Fructus Persicae of 2.5-4.5%, the Herba Cymbopogonis Citrari mix homogeneously of 2-3%, boiling is extracted 2 times, adds 10-15 times amount water for the first time, the
Secondary adds 5-10 times amount water, merges decoction liquor, filters, is concentrated into the 1/4-1/3 of original volume, is subsequently adding and is equivalent to concentrated solution
The polyethylene glycol oxide of weight 15-20%, the polyacrylamide of 10-15%, the hydroxypropyl methyl cellulose of 4-8%, 3-5% xanthan gum,
The sodium alginate of 4-6%, stirs, and is uniformly sprayed at by the colloid solution obtained on the stone powder that step (1) prepares, dries
Dry, it is cooled to room temperature；After transplanting by pressurized circulation pump by nutritional solution in droping irrigating pipe is input to flowerpot, 1 morning weekly
Drip irrigation 20-30 minute, all 3 the morning drip irrigation 20-30 minute, remaining time uses clear water drip irrigation；The composition of described nutritional solution contains
Amount is: potassium nitrate 0.3-0.5 g/L, calcium nitrate 0.2-0.4g/L, magnesium sulfate 0.1-0.2g/L, ammonium phosphate 0.2-0.3g/L, sulfur
Acid potassium 0.25-0.35g/L, potassium dihydrogen phosphate 0.1-0.2g/L, b diammonium disodium edta 0.01-0.02g/L, ferrous sulfate
0.06-0.08g/L, boric acid 0.005-0.015g/L, manganese sulfate 0.015-0.025g/L, zinc sulfate 0.006-0.012g/L, sulfur
Acid copper 0.2-0.3mg/L, ammonium molybdate 0.05-0.1mg/L；Nutritional solution accomplishes that nutrient tank wants lucifuge with joining and can not deposit；
(4) factorial praluction is placed in warmhouse booth growth by potted plant for Folium Stevlae Rebaudianae, by pinching, pinches, controls water, cuts root, control
Plant height, reaches potted plant and views and admires purpose；
1. pinching: mainly extract the bud point most with apical dominance, make growing tips of the plant change, promote induction lateral bud, reach
The purpose that plant is plentiful, downgrade；
2. pinch: refer to that easy spindling plant is too fast because of growth, promote plant nutrient dispersion to supply by wiping out a part of apical meristem
Give；
3. control water: when growing vigorous, control the artificial plant that makes of the water yield and be in semiarid state, reach that plant is short and small, plentiful, side
Branch is flourishing；
4. cut root: cut off main root by artificial instrument, control growth；
(5) finished product goes out garden.
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| CN104311323A (en) * | 2014-10-20 | 2015-01-28 | 柳州市天姿园艺有限公司 | Nutrient oil for flowers |
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| CN105660150B (en) * | 2016-03-02 | 2018-05-25 | 黄山市芽典生态农业有限公司 | A kind of nutritive cube cultural method of Huangshan Gongju |
| CN106258805A (en) * | 2016-08-13 | 2017-01-04 | 安庆市老杨埠农业科技有限公司 | A kind of method for transplanting of margosa seedling |
| CN106305397A (en) * | 2016-08-25 | 2017-01-11 | 合肥市田然农业科技园有限公司 | Soilless culture method of catharanthus roseus |
| CN106631556A (en) * | 2016-11-03 | 2017-05-10 | 明光市大全甜叶菊专业合作社 | Stevia mother-root medium for temporary planting |
| CN113455304A (en) * | 2021-07-12 | 2021-10-01 | 李辉 | Potted plant domestication method for traditional Chinese medicine saffron |
| CN113455303A (en) * | 2021-07-12 | 2021-10-01 | 李辉 | Potting domestication method for traditional Chinese medicinal material chrysanthemum morifolium |
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