CN108770623A - The overwintering no suspend mode hardening off method of bletilla tissue culture seedlings duplicature - Google Patents
The overwintering no suspend mode hardening off method of bletilla tissue culture seedlings duplicature Download PDFInfo
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- CN108770623A CN108770623A CN201810655789.2A CN201810655789A CN108770623A CN 108770623 A CN108770623 A CN 108770623A CN 201810655789 A CN201810655789 A CN 201810655789A CN 108770623 A CN108770623 A CN 108770623A
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- China
- Prior art keywords
- seedling
- tissue culture
- bletilla
- overwintering
- hardening
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Abstract
The present invention discloses a kind of overwintering no suspend mode hardening off method of bletilla tissue culture seedlings duplicature, specifically includes following steps：The building method of the condition of culture during hardening and plastics Small plastic shed is described in detail in the child care of tissue-cultured seedling, preparing seedbed, transplanting breeding；The present invention does not have hibernation period when realizing bletilla tissue culture seedlings hardening, shortens tissue-cultured seedling planting time, improves the planting percent and quality of bletilla tissue culture seedlings, simplifies hardening flow, facilitates management, cost-effective.
Description
Technical field
The invention belongs to Chinese herbal medicine seedling cultivation fields, more particularly to a kind of overwintering nothing of bletilla tissue culture seedlings duplicature
Suspend mode hardening off method.
Background technology
The medicinal plant bletilla striata (scientific name：Bletilla striata), it is a kind of herbaceos perennial that the orchid family bletilla striata belongs to,
Underground shape stem, triangular shape, rich in sticky bletilla mannan, can hyoscine, have the benefits of hemostasis tonifying lung, myogenic pain relieving；With city
The increase of field demand, people excessively excavate wild bletilla striata, cause wild resource exhausted, the research of the artificial cultivation bletilla striata at present is
It is increasingly ripe, the demand of tissue-cultured seedling is gradually increased, expands breeding seedling using artificial tissue-cultured seedling and supplies market, improve bletilla striata yield
And quality, to meet the market demand.
During training tissue culture seedling, with the reduction of winter temperature, the bletilla striata is fallen leaves to stop growing into rest period, stops
During dormancy, some smaller seedlings, in stem tuber storage nutrient be consumed when overwintering it is idle to the greatest extent, cause dead seedling, affect planting percent；
So there is an urgent need for studying a kind of overwintering hardening cultural method of bletilla tissue culture seedlings, the bletilla striata can also be grown in winter for breaking dormancy, improves
Planting percent reduces cultivation management cost, and is conducive to manual operation and improves production efficiency.
Invention content
The purpose of the present invention is to provide a kind of overwintering no suspend mode hardening off method of bletilla tissue culture seedlings duplicature, to realize the bletilla striata
There is no hibernation period during training tissue culture seedling, shortens tissue-cultured seedling planting time, improve the planting percent of tissue-cultured seedling, reduce cultivation
Train cost.
The invention discloses the overwintering no suspend mode hardening off method of bletilla tissue culture seedlings duplicature, specifically include following steps：
Step 1：Using the bottled seedling of taking root of the bletilla striata of bletilla striata stem tip tissue culture, the culture medium of root is washed away, is then planted
It is implanted in pearlite interstitial substance, strain, line-spacing are 2cm × 3cm, and conventional fertilizer and water management, child care 30~40 days are carried out in greenhouse；
Step 2：When seedling grows to 5cm~10cm high, transplants and cultivated on the seedbed put in order in greenhouse；
Step 3：Bletilla striata seedling is cultivated in the fall, and sprout tumble does not occur in covered plastic Small plastic shed, entire winter bletilla striata seedling,
Underground stem tuber not suspend mode, continued growth are overwintering；Growth time extends 3 months, cultivate the seedling stem tuber diameter of half a year up to 2~
2.5cm；10~15cm of height of seedling, 5~8cm of root long, root of hair number 5~10 can be transplanted or be sold；
Further, the bottled 3~4cm of seedling height of seedling of taking root of step 1 bletilla striata, stem tuber diameter 0.5cm, root long 2~
3cm, root of hair number 3~6；Perlite moisture content is maintained at 60%~70% during child care, and the relative humidity of air is 80%~
90%, for temperature at 15 DEG C~30 DEG C, illumination light transmittance is 20%~30%, and mass concentration of every 7 days sprays is 0.2%
Compound solution, each component content ratio is N in compound solution:P2O5:K2O=12:8:12；
Further, the specific method of preparing seedbed is when transplanting greenhouse in the step 2：First soil deeptillage, per acre
It applies 4000kg organic fertilizers and makees base fertilizer, rotary tillage 1 time is sealed 15~20 days, then rotary tillage 1 time, is made loosing soil, smooth by site preparation, is pressed
1.2m manages moisture in the soil, and the high 20-25cm of moisture in the soil opens the seed furrow of 30cm；
Further, when greenhouse is cultivated in the step 2, soil moisture is maintained at 40%~50%, air it is relatively wet
Degree is 70%~90%, and temperature is at 15 DEG C~32 DEG C, and illumination light transmittance is 30%~40%, 10~15 days one inferior qualities of spray
A concentration of 0.2% compound solution is cooked top dressing, and each component content ratio is N in compound solution:P2O5:K2O=12:8:12；
Further, the method for construction of the step 3 plastics Small plastic shed is as follows：Bamboo chip, fine steel rib or mulberry are used in moisture surface
Item builds the plastics Small plastic shed of one layer of 60~80cm high on the face of seedbed ridge.
The beneficial effects of the invention are as follows：1. facility source used in hardening off method of the present invention is extensive, easy to operate, at low cost；
2. the present invention increases photo-thermal using bilayer film canopy absorbs space, winter temperature of shed, humidity are improved, tissue-cultured seedling is improved
Planting percent and quality；3. the present invention makes tissue-cultured seedling not suspend mode in winter, continued growth shorten tissue culture using bilayer film canopy
The cultivation period of seedling makes hardening process simplification, facilitates management, cost-effective.
Description of the drawings
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technology description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
Obtain other attached drawings according to these attached drawings.
Fig. 1 is double membrane structure figure；
Wherein 1. greenhouses, 2. plastics Small plastic sheds.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
The invention discloses a kind of overwintering no suspend mode hardening off method of bletilla tissue culture seedlings duplicature, and this method specifically includes following
Step：
Step 1：Using the bottled seedling of taking root of the bletilla striata of bletilla striata stem tip tissue culture, the culture medium of root is washed away, is then planted
It is implanted in pearlite interstitial substance, strain, line-spacing are 2cm × 3cm, and conventional fertilizer and water management, child care 30~40 days are carried out in greenhouse 1；
The wherein bottled 3~4cm of seedling height of seedling of taking root of the bletilla striata, stem tuber diameter 0.5cm, 2~3cm of root long, root of hair number 3~6,
Perlite moisture content is maintained at 60%~70% during child care, and the relative humidity of air is 80%~90%, and temperature is 15 DEG C~30
DEG C, illumination light transmittance is 20%~30%, the compound solution that mass concentration of every 7 days sprays is 0.2%, each in compound solution
Constituent content ratio is N:P2O5:K2O=12:8:12；
Step 2：When seedling grows to 5cm~10cm high, transplants and cultivated on the seedbed put in order in greenhouse 1；
First soil deeptillage, applies 4000kg organic fertilizers and makees base fertilizer, rotary tillage 1 time is sealed 15~20 days, then revolved per acre before transplanting
It ploughs 1 time, by careful site preparation, makes loosing soil, smooth, by 1.2m reason moisture in the soils, the high 20~25cm of moisture in the soil opens the seed furrow of 30cm, with
Just cultivation management；
Seedbed after transplanting：Soil moisture is maintained at 40%~50%, and the relative humidity of air is 70%~90%, and temperature exists
15 DEG C~32 DEG C, illumination light transmittance is 30%~40%, the compound solution that mass concentration of 10~15 days sprays is 0.2%
Top dressing is done, each component content ratio is N in compound solution:P2O5:K2O=12:8:12；
Step 3：Bletilla striata seedling is cultivated in the fall, and covered plastic Small plastic shed 2, entire winter bletilla striata seedling does not fall
Seedling, underground stem tuber not suspend mode, continued growth are overwintering；Growth time extends 3 months, cultivate the seedling stem tuber diameter of half a year up to 2~
2.5cm；10~15cm of height of seedling, 5~8cm of root long, root of hair number 5~10 can be transplanted or be sold.
The structure chart of duplicature is as shown in Figure 1, the method for construction of plastics Small plastic shed 2 is as follows：In upper bamboo chip, thin steel above
Muscle or mulberry shoot build the plastics Small plastic shed 2 of one layer of 60~80cm high on the face of seedbed ridge, and 2 raw material sources of plastics Small plastic shed are extensive,
It makes simply, is suitable for the overwintering nursery application in various seedbeds.
During training tissue culture seedling, as unit of moisture in the soil, to establish plastics Small plastic shed 2 in greenhouse, allow heat into it is how scattered
It is few, sunlight energy is made full use of, temperature, moisture stable in plastics Small plastic shed 2 are kept, good ring is created for tissue-cultured seedling growth
Border allows tissue-cultured seedling in winter without rest period, can normal growth, to improve the bletilla tissue culture seedlings speed of growth and planting percent,
The cultivation time for shortening bletilla tissue culture seedlings, improve the yield and quality of bletilla tissue culture seedlings.
Embodiment
The training of root is washed away using the bottled seedling of the bletilla striata that takes root of bletilla striata stem tip tissue culture in Plant Tissue Breeding room
Support base, then in seedlings nursing plate, the row with perlite into seeding row spacing 2cm × 3cm is close, carried out in greenhouse conventional fertilizer and water management into
Row child care；Child care 30~40 days is transplanted on the seedbed put in order in greenhouse and is cultivated when height of seedling is 5cm~10cm；Training
The plastics Small plastic shed for being capped the present invention in the fall is educated, winter child care is carried out, makes seedling winter not sprout tumble, can continue to give birth to
It is long, improve planting percent；
The present invention has carried out contrast test in academy of agricultural sciences of Yunnan Province proving ground；Carried out in greenhouse A of the present invention with often
Rule do not take hut hardening off method B cultivation contrast test, experimental field, greenhouse experiment it is completely the same；
1, trial crops
The bletilla striata, the tissue-cultured seedling of child care 30~40 days are transplanted on cultivation matrix and carry out when seedling grows to 5cm~10cm high
Culture experiment；
2, test method
Experiment is unified by each processing plot area 1m × 10m=10m2, cultivation row, strain spacing are 10cm, each to locate
Manage cell and three repeat regions be set, be colonized 1000 plants altogether, A groups of the present invention with do not take routinely it is normal during hut B group hardenings
It is consistent to advise management measure；On June 20th, 2016 transplants, two total A groups, B groups processing, 6 regions；
A groups of the present invention take hut on November 20th, 2016, carry out the overwintering child care of duplicature, withdraw on 2 20th, 2017 small
Canopy, child care 3 months；Routine does not take hut B groups and does not build hut during on November 20th, 2016 on 2 20th, 2017,
His breeding condition is identical as A groups of the present invention, cultivates seedling taking on June 20 2017 1 anniversary and is investigated；
3, investigation method：
Sampling survey biological characteristics：It cultivated for 1 anniversary, respectively takes 100 young plants to investigate from 6 regions on June 20th, 2017
Planting percent and biological characteristics；Fertilizer, pesticide and the water rate that the statistics anniversary uses per acre, which amount to, calculates cost, by seedling
The bletilla striata seedling of growing way A groups of the present invention has just reached emergence standard in 25 days~May 5 April in 2017, plant height up to 15~20cm,
Stem tuber diameter is up to the strong sprout standard of 1.5~2cm, seedling time advance 40~45 days；
4, test result refers to table 1, table 2：
1 field trial growth of cereal crop seedlings investigation result statistical form 2017.6.20 of table
2 field trial cost management result investigation statistics table of table
Planting percent：I, the planting percent average value of tri- repeat regions of II, III；Cost：What fertilizer, pesticide and water rate were put into
It amounts to；Management is artificial：Ponding is handled, the artificial number of rotten seedling and chemical control is removed；
The overwintering no suspend mode hardening off method of bletilla tissue culture seedlings duplicature is compared with common hardening off method, bletilla tissue culture seedlings seedling
Rate improves 31.1%, and cost reduces 35%, and management manually reduces 50%, and death rate reduces 54%, seedling time advance
40~45 days, the hardening time of the bletilla striata is shortened, improves production efficiency, the planting percent of the bletilla striata and quality.
Each embodiment in this specification is all made of relevant mode and describes, identical similar portion between each embodiment
Point just to refer each other, and each embodiment focuses on the differences from other embodiments.Especially for system reality
For applying example, since it is substantially similar to the method embodiment, so description is fairly simple, related place is referring to embodiment of the method
Part explanation.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the scope of the present invention.It is all
Any modification, equivalent replacement, improvement and so within the spirit and principles in the present invention, are all contained in protection scope of the present invention
It is interior.
Claims (5)
1. the overwintering no suspend mode hardening off method of bletilla tissue culture seedlings duplicature, which is characterized in that this method specifically includes following steps：
Step 1：Using the bottled seedling of taking root of the bletilla striata of bletilla striata stem tip tissue culture, the culture medium of root is washed away, is then planted in
In pearlite interstitial substance, strain, line-spacing are 2cm × 3cm, and conventional fertilizer and water management, child care 30~40 days are carried out in greenhouse；
Step 2：When seedling grows to 5cm~10cm high, transplants and cultivated on the seedbed put in order in greenhouse；
Step 3：Bletilla striata seedling is cultivated in the fall, and sprout tumble does not occur in covered plastic Small plastic shed (2), entire winter bletilla striata seedling,
Underground stem tuber not suspend mode, continued growth are overwintering；Growth time extends 3 months, cultivate the seedling stem tuber diameter of half a year up to 2~
2.5cm；10~15cm of height of seedling, 5~8cm of root long, root of hair number 5~10 can be transplanted or be sold.
2. the overwintering no suspend mode hardening off method of bletilla tissue culture seedlings duplicature according to claim 1, which is characterized in that the step
Bottled 3~the 4cm of seedling height of seedling of taking root of rapid 1 bletilla striata, stem tuber diameter 0.5cm, 2~3cm of root long, root of hair number 3~6；During child care
Perlite moisture content is maintained at 60%~70%, and the relative humidity of air is 80%~90%, and for temperature at 15 DEG C~30 DEG C, illumination is saturating
Luminosity is 20~30%, the compound solution that mass concentration of every 7 days sprays is 0.2%, each component content ratio in compound solution
For N:P2O5:K2O=12:8:12.
3. the overwintering no suspend mode hardening off method of bletilla tissue culture seedlings duplicature according to claim 1, which is characterized in that the step
The specific method of preparing seedbed is when transplanting greenhouse in rapid 2：First soil deeptillage applies 4000kg organic fertilizers and makees base fertilizer per acre, rotation
It ploughs 1 time, seals 15~20 days, then rotary tillage 1 time, make loosing soil, smooth by site preparation, by 1.2m reason moisture in the soils, the high 20~25cm of moisture in the soil is opened
The seed furrow of 30cm.
4. the overwintering no suspend mode hardening off method of bletilla tissue culture seedlings duplicature according to claim 1, which is characterized in that the step
When greenhouse (1) is cultivated in rapid 2, soil moisture is maintained at 40%~50%, and the relative humidity of air is 70%~90%, and temperature exists
15 DEG C~32 DEG C, illumination light transmittance is 30%~40%, and the compound solution of mass concentration 0.2% of 10~15 days sprays chases after
Fertilizer, each component content ratio is N in compound solution:P2O5:K2O=12:8:12.
5. the overwintering no suspend mode hardening off method of bletilla tissue culture seedlings duplicature according to claim 1, which is characterized in that the step
The method of construction of rapid 3 plastics Small plastic shed (2) is as follows：In moisture surface one is built on the face of seedbed ridge with bamboo chip, fine steel rib or mulberry shoot
The plastics Small plastic shed (2) of 60~80cm high of layer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810655789.2A CN108770623B (en) | 2018-06-23 | 2018-06-23 | Bletilla striata tissue culture seedling double-layer film overwintering non-dormancy seedling hardening method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810655789.2A CN108770623B (en) | 2018-06-23 | 2018-06-23 | Bletilla striata tissue culture seedling double-layer film overwintering non-dormancy seedling hardening method |
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| Publication Number | Publication Date |
|---|---|
| CN108770623A true CN108770623A (en) | 2018-11-09 |
| CN108770623B CN108770623B (en) | 2021-01-29 |
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|---|---|---|---|
| CN201810655789.2A Expired - Fee Related CN108770623B (en) | 2018-06-23 | 2018-06-23 | Bletilla striata tissue culture seedling double-layer film overwintering non-dormancy seedling hardening method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109744116A (en) * | 2019-03-25 | 2019-05-14 | 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) | A kind of overwintering method of keeping a full stand of seedings of sweet potato seedling |
| CN111758467A (en) * | 2019-08-07 | 2020-10-13 | 东北林业大学 | Method for relieving non-physiological dormancy in tissue culture seedling transplanting process of fraxinus mandshurica |
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| CN106305106A (en) * | 2016-08-24 | 2017-01-11 | 四川七彩林业开发有限公司 | Method for tissue-culture seedling exercising of callicarpa bodinieri |
| CN106342517A (en) * | 2016-08-24 | 2017-01-25 | 四川七彩林业开发有限公司 | Melaleuca bracteata tissue culture seedling hardening method |
| CN107173222A (en) * | 2017-03-27 | 2017-09-19 | 河池乐康生态农业科技有限公司 | A kind of wild bletilla striata quick breeding method for tissue culture |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109744116A (en) * | 2019-03-25 | 2019-05-14 | 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) | A kind of overwintering method of keeping a full stand of seedings of sweet potato seedling |
| CN111758467A (en) * | 2019-08-07 | 2020-10-13 | 东北林业大学 | Method for relieving non-physiological dormancy in tissue culture seedling transplanting process of fraxinus mandshurica |
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| CN108770623B (en) | 2021-01-29 |
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