CN106305106A - Method for tissue-culture seedling exercising of callicarpa bodinieri - Google Patents
Method for tissue-culture seedling exercising of callicarpa bodinieri Download PDFInfo
- Publication number
- CN106305106A CN106305106A CN201610716690.XA CN201610716690A CN106305106A CN 106305106 A CN106305106 A CN 106305106A CN 201610716690 A CN201610716690 A CN 201610716690A CN 106305106 A CN106305106 A CN 106305106A
- Authority
- CN
- China
- Prior art keywords
- seedling
- transplanting
- illumination
- tissue
- tissue cultured
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
Landscapes
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Organic Chemistry (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention relates to the technical field of tissue-culture seedling culture, and particularly discloses a method for tissue-culture seedling exercising of callicarpa bodinieri. The method comprises the following steps of selecting a tissue-culture container seedling of the callicarpa bodinieri meeting the special requirement, and sequentially performing container closing domesticating and container opening domesticating under the special environment conditions; after domesticating, transplanting into a seedling bed, and exercising the seedling; transplanting into a seedling culture bag, and managing. The method has the advantages that the multi-aspect control and management are performed in the tissue-culture seedling exercising process of the callicarpa bodinieri, and the obtained callicarpa bodinieri seedling has good growth condition, thick stem, and high survival rate; the reasonable substrate formula is selected, the loosening, air ventilating and water-accumulation preventing effects are realized, the moisture required by the growth is guaranteed, the tissue-culture seedling can favorably grow with new root, and the survival rate is improved; the rooting of the tissue-culture seedling is good, the survival rate of seedling exercising is high, the survival rate of transplanting is high, the features and characteristics of the excellent variety are well maintained, the method can be directly applied to production, and the economic benefit, social benefit and ecological benefit are better.
Description
Technical field
The present invention relates to tissue cultivating and seedling technical field, specifically, a kind of method relating to Folium Callicarpae Formosanae training tissue culture seedling.
Background technology
Folium Callicarpae Formosanae has another name called Callicarpa dichotoma, Folium Callicarpae Formosanae, for Verenaceae Callicarpa machaka, and plant height 1.2 meters to about 2 meters,
Sprig is smooth, slightly aubergine, has a small amount of stellate hair, and single leaf is to life, blade obovate to ellipse, length 7 centimetres to 15 lis
Rice, tip is tapering, and raw serration is dredged at edge.Cyme axil is raw, the total stalk of tool, spends majority, alabastrum purple or pink, and flower has
White, pink colour, pale purple isochrome, 6 to July is open.Fruit is spherical, in purple after 9 to October maturation, glossy, do not fall through the winter.Purple
Pearl happiness is warm, it is wet to like, aversion to wind, fearness drought, preferable in shady and cool ambient growth.It is many that Folium Callicarpae Formosanae sprouts bar, and root system is the most flourishing, for shallow root tree
Kind.Folium Callicarpae Formosanae strain shape is beautiful, and pattern is gorgeous, and fruit is bright in luster, elegant and polished, just as the Margarita of many purples, be one both
Considerable flower can appreciate again the excellent flower variety of fruit, is usually used in afforestation or garden plantation, it is possible to potted plant view and admire.Its fruit ear also may be used
Cut bottle insert or make cut-flower material.Folium Callicarpae Formosanae root or Herb can be used as medicine, and have the effect such as hemostasis, antibacterial, heat-clearing and toxic substances removing.Therefore Folium Callicarpae Formosanae
There is the highest gardening ornamental value and pharmaceutical value.
Folium Callicarpae Formosanae breeding can be bred by tissue culture mode, and its technology is the most ripe, can carry out large-scale production, be trained by group
Mode can obtain substantial amounts of Folium Callicarpae Formosanae tissue cultured seedling.Folium Callicarpae Formosanae tissue cultured seedling grows into certain altitude in group training room and just to transplant,
Tissue culture bottle moves on to outdoor again to booth from group training room, tissue cultured seedling from tissue culture bottle to seedbed or in container, grows into from culture medium
Smoothly transitting to grow inside substrate, whole process is called for short training tissue culture seedling.The purpose of seedling exercising is to improve tissue cultured seedling to external world
The adaptability of environmental condition, improves its photosynthetic ability, promotes tissue cultured seedling healthy and strong, is finally reached raising tissue cultured seedling and transplants into
The purpose of motility rate.
Also there is not concrete Folium Callicarpae Formosanae hardening off method at present, be unfavorable for the large-scale production of Folium Callicarpae Formosanae tissue cultured seedling.Therefore, one it is badly in need of
Plant the method being effectively improved Folium Callicarpae Formosanae seedling exercising survival rate.
Summary of the invention
In order to solve problems of the prior art, it is an object of the invention to provide the side of a kind of Folium Callicarpae Formosanae training tissue culture seedling
Method.
In order to realize the object of the invention, technical scheme is as follows:
A kind of method that the invention provides Folium Callicarpae Formosanae training tissue culture seedling, comprises the steps:
S1, selection plant height 6～8cm, blade >=5 slice, root length >=3cm, the Folium Callicarpae Formosanae tissue cultured seedling bottle Seedling of radical >=5 piece；
S2, close bottle domestication:
S21, tissue cultured seedling bottle Seedling is moved to the outdoor placement 2d of intensity of illumination 1000～2000lux, temperature 20～28 DEG C；Mesh
Be: the environment allow tissue cultured seedling be transitioned into outdoor from group training room environment aseptic, constant temperature and have bacterium, having the temperature difference, outdoor has
Shelter from heat or light the condition ventilated, be the process of progressively transition；
S22, again tissue cultured seedling bottle Seedling is moved to intensity of illumination 2000～3000lux, temperature 20～28 DEG C booth place 3d；
S3, corkage domestication:
Pine lid 2d, then part is uncapped 1d, and last uncapping completely places 1～2d, booth illumination control 2000～
3000lux, temperature is at 20～28 DEG C, and air humidity controls more than 70%.
As preferably, in S21, tissue cultured seedling bottle Seedling is moved to intensity of illumination 1800lux, daylight temperature 25～28 DEG C, night
Temperature 20～the outdoor of 22 DEG C.
As preferably, in S22, tissue cultured seedling bottle Seedling is moved to intensity of illumination 2500lux, daylight temperature 25～28 DEG C, night
Temperature 20～the booth of 22 DEG C.
Carry out under these conditions closing bottle domestication, it is possible to allow Folium Callicarpae Formosanae tissue cultured seedling progressively adapt to outdoor environment, it may be observed that purple
Pearl tissue cultured seedling has grown, and leaf color deepens, and stem stalk is thicker, and root system is elongated, and plant is more healthy and stronger.
As preferably, in S3, booth illumination controls at 2500lux, and daylight temperature controls at 25～28 DEG C, nocturnal temperature control
System is at 20～22 DEG C.Folium Callicarpae Formosanae tissue cultured seedling is allowed in progressively adapting to booth, to have the environment of bacterium, alternating temperature, high light, it may be observed that tissue cultured seedling is more
Add healthy and strong tall and straight, root system is more flourishing, has the most possessed the ability transplanting seedbed oneself growth.
Further, described method also comprises the steps:
S4, by complete corkage domestication tissue cultured seedling clean up after be transplanted to seedling exercising in seedbed, seedling exercising substrate is equal-volume
Than fertile soil and the perlite of mixing, seedling exercising soil humidity controls 50～60%；
S5, the seedling of the plant height >=15cm survived by seedling exercising are transplanted in non-woven fabrics Seedling bag, transplanting medium by fertile soil,
Vermiculitum and perlite mix according to volume ratio 2:1:1, and transplanting medium humid control is 50～60%.
Folium Callicarpae Formosanae seedling for transplanting should meet plant height and reach more than 10cm, and root growth is good, has young leaves to grow, the number of blade
Increasing leaf color light green, stem is sturdy.
Select non-woven fabrics Seedling bag as transplanting container, fill rear diameter 10cm, high 12cm, non-woven fabrics have ventilative, can
The feature of degraded, little seedling rooting and later stage carrying after being beneficial to transplant, lower.
Further, in S4, during transplanting, seedbed air humidity controls more than 70%, and air temperature control is 20～28
DEG C, intensity of illumination controls 2000～3000lux；Controlling air humidity after transplanting in 7d is 85～95%, and illumination controls
2000～3000lux；After transplanting, 8～14d control air humiditys are 80～85%, and illumination controls 3000～4000lux；Transplant
Rear 15～20d control air humiditys are 70～80%, and illumination controls 4000～5000lux；After transplanting, 25～34d illumination control
5000～7000lux, humidity is natural humidity；After transplanting, 35～40d illumination control 7000～9000lux, and humidity is certainly
So humidity；Nursery bed temperature remains at 22～30 DEG C.
Further, S4 comprises the steps: during transplanting
S41, clean up tissue cultured seedling root, in 700～900 times of carbendazols of dilution, soak 4～6min；
S42, according to line-spacing 6cm, spacing in the rows 5cm is transplanted, and first digs 2～4cm deep duck eyes during transplanting, be then placed in group training
Seedling, unfolds root system, and the degree of depth is advisable so that blade not contacting substrate and root-stem transition zone are equal with substrate face, waters, then after transplanting
Spray 1000 times of carbendazols of dilution.
Further, after transplanting 7d, 0.1% carbamide and antibacterial (carbendazim or Mancozeb etc.), 5d are sprayed
After spray 0.1% dipotassium hydrogen phosphate, alternately spray 0.2% carbamide and 0.2% dipotassium hydrogen phosphate every 5d later.Use after about 20d
0.2% compound fertilizer's aqueous solution interval 7d waters.
Further, the ground that S5 transplants should draining good, soil water-retaining and not hardening, there is no weeds.For transplant
Folium Callicarpae Formosanae seedling should meet plant height and reach more than 15cm, and root growth is flourishing, and leaf color is dark green, and stem is sturdy, well-grown.Transplanting should be selected
Overcast and rainy, suitably shelter from heat or light in 10d after transplanting, it is to avoid high light directly shines, carry out pest management, water in time and hoe up weeds.
Can apply fertilizer after transplanting 10d, when fertilization principle is for growing vigorous, execute N P and K and other elements more, enter branch
Hardening period, phosphorus was auxiliary based on potassium, promoted children's stem lignifying, in order to survive the winter.
The beneficial effects of the present invention is:
The present invention by carrying out many-sided control and management to Folium Callicarpae Formosanae training tissue culture seedling process, and gained Folium Callicarpae Formosanae seedling growth is good
Good, stem stalk is sturdy, survival rate is high.
The reasonable matrix formulations that the present invention selects, i.e. loose ventilative not hydrops, can guarantee that again growth required moisture, beneficially group
Seedlings cultivating length makes new advances root, improves survival rate.
Hardening off method tissue cultured seedling of the present invention is taken root well, seedling exercising survival rate high, transplanting survival rate is high, can be good
Retain the feature of excellent strain, may be directly applied to produce, have preferable economic benefit, social benefit and Ecological Effect
Benefit.
Accompanying drawing explanation
Fig. 1 is the Folium Callicarpae Formosanae tissue cultured seedling in the embodiment of the present invention 1 for seedling exercising.
Fig. 2 is the Folium Callicarpae Formosanae tissue cultured seedling preparing in the embodiment of the present invention 1 to transplant.
Fig. 3 is the Folium Callicarpae Formosanae tissue cultured seedling being grown in seedbed in the embodiment of the present invention 1.
Detailed description of the invention
Below in conjunction with embodiment, the preferred embodiment of the present invention is described in detail.It will be appreciated that following reality
Execute providing merely to play descriptive purpose of example, be not used to the scope of the present invention is limited.The skill of this area
Art personnel, in the case of without departing substantially from spirit of the invention and spirit, can carry out various amendment and replacement to the present invention.
Experimental technique used in following embodiment if no special instructions, is conventional method.
Material used in following embodiment, reagent etc., if no special instructions, the most commercially obtain.
Embodiment 1
(1) hot house and the requirement in seedbed
1, hot house should possess inside and outside sunshade net, fan and pad, automatic spraying system and control system.Have below seedbed
Drainage system, about the width 1-1.2 rice of seedbed, convenient operation.
(2) tissue cultured seedling seedbed seedling exercising
1, seedling exercising tissue cultured seedling prescription
Plant height reaches 6-8cm, more than 5, blade, and more than root length 3cm, radical more than 5, well developed root system, leaf color is dark green, children
Seedling stalwartness is tall and straight.Get rid of the undesirable tissue cultured seedlinies such as thin and weak Seedling, etiolated seedling, aging Seedling and deformity Seedling.
2, the selection of seedling exercising substrate and process
Selecting fertile soil and perlite to mix as seedling exercising substrate according to volume ratio 1:1 ratio, be laid on seedbed, thickness is
12-14cm, with antibacterial sufficiently steriliseds such as 800 times of carbendazim, places 3d and uses.Soil humidity controls at 50-60%
3, domestication before tissue cultured seedling is transplanted
(1) close bottle domestication: when tissue cultured seedling grows to meet seedling exercising requirement, tissue culture bottle is moved on to group training outdoor ventilation and shelters from heat or light place
Closing a bottle seedling exercising, intensity of illumination controls at 1000-2000lux, and temperature, at 20-28 DEG C, is put into outdoor about 2d, then is transported to booth and puts
Putting about 3d, booth intensity of illumination controls at 2000-3000lux, and whole process progressively strengthens illumination, it is to avoid high light directly shines or prominent
So strengthen, observe growth of seedling situation at any time.
(2) open domestication: after closing bottle domestication, bottleneck lid progressively opened and carries out corkage domestication, i.e. first pine lid 2d,
Then part is uncapped 1d, and last uncapping completely places 1-2d, and booth illumination controls at 2000-3000lux, temperature at 20-28 DEG C,
Air humidity controls more than 70%.Taming successful standard is that tissue cultured seedling grows up, and stem and leaf color burn, root system is more sent out
Reaching, seedling is the most healthy and the strongest tall and straight, just transplants after having tamed.
4, the transplanting of tissue cultured seedling
(1) seedling exercising is preferably carried out in the 4-8 month, transplants and is preferably selected in the afternoon or carries out at dusk, and during transplanting, seedbed air humidity should
Controlling more than 70%, temperature controls at 20-28 DEG C, and intensity of illumination controls at 2000-3000lux.
(2) to beat easily when taking out seedling from bottle and put down gently, be careful not to lose root system and stem and leaf.With tweezers, seedling is taken out
Put into the culture medium cleaning root attachment in clear water, then tissue cultured seedling root is put into immersion 5min in 800 times of carbendazols.
(3) according to line-spacing 6cm, spacing in the rows 5cm is transplanted, and first digs the deep duck eye of 2-4cm, be then placed in tissue cultured seedling during transplanting,
Unfolding root system, the degree of depth is advisable so that blade not contacting substrate and root-stem transition zone are equal with substrate face.Transplant while covering base
Matter, is compacted the most gently, sprays water, and injection flow rate wants suitable.1000 times of carbendazim medicinal liquids are sprayed after transplanting.
5, seedbed management
(1) humidity
The present invention ensures air humidity by full-automatic spraying system, ensures that air humidity is that 8-14d is wet after transplanting in 7d
Degree controls at 80-85%, and 15-20d humid control is at 70-80%, and medium of seedling bed humid control is at 50-60%.Observe children at any time
Seedling growing state, after having observed that new root length goes out, gradually reduces air humidity, adapts to booth humidity.Observe substrate at any time wet
Degree, controls the irrigation amount of whole process well.
(2) temperature
Nursery bed temperature is allowed to be maintained at 22-30 DEG C by sheltering from heat or light, ventilate and regulate the measures such as humidity.
(3) illumination
Transplant illumination in 7d and control at 2000-3000lux, 8-14d be 3000-4000lux, 15-20d be 4000-
5000lux, 25-34d be 5000-7000lux, 34-40d be 7000-9000lux.
(4) apply fertilizer
After transplanting after transplanting 7d, spray 0.1% carbamide, after 5d, spray 0.1% dipotassium hydrogen phosphate, interval 5d alternately spray
Water with 0.2% compound fertilizer's aqueous solution interval 7d after executing 0.2% carbamide or 0.2% dipotassium hydrogen phosphate, about 20d.
(5) the prevention and control of plant diseases, pest control
Pest and disease damage is to put prevention first.After tissue cultured seedling transplants 1 week, every 7d sprays a sterilization such as carbendazim or Mancozeb
Agent.Observe seedling at any time to encroach on or without disease pest, process in time.Remove seedbed undergrowth, dead tissue cultured seedling and weeds, keep
Seedbed neat and tidy.
It is 85% through statistics Folium Callicarpae Formosanae training tissue culture seedling survival rate.
(3) little transplantation of seedlings and management are survived
1, Folium Callicarpae Formosanae survives the standard of little transplantation of seedlings
Plant height reaches more than 15cm, and root growth is flourishing, and leaf color is dark green, and stem is sturdy, well-grown.
2, the management behind lower ground
Transplanting should be selected in overcast and rainy, suitably shelters from heat or light after transplanting in 10d, it is to avoid high light directly shines, and carries out pest management, in time
Water and hoe up weeds.Can apply fertilizer after transplanting 10d, when fertilization principle is for growing vigorous, execute N P and K and other elements more, enter
Branch hardening period, phosphorus was auxiliary based on potassium, promoted children's stem lignifying, in order to survive the winter.
Through statistics lower ground, survival rate is 90%.
Comparative example 1
(1) hot house and the requirement in seedbed
1, hot house should possess inside and outside sunshade net, fan and pad, automatic spraying system and control system.Have below seedbed
Drainage system, about the width 1-1.2 rice of seedbed, convenient operation.
(2) tissue cultured seedling seedbed seedling exercising
1, seedling exercising tissue cultured seedling prescription
Plant height reaches 6-8cm, more than 5, blade, and more than root length 3cm, radical more than 5, well developed root system, leaf color is dark green, children
Seedling stalwartness is tall and straight.Get rid of the undesirable tissue cultured seedlinies such as thin and weak Seedling, etiolated seedling, aging Seedling and deformity Seedling.
2, the selection of seedling exercising substrate and process
Selecting fertile soil and perlite to mix as seedling exercising substrate according to volume ratio 1:1 ratio, be laid on seedbed, thickness is
12-14cm, with antibacterial sufficiently steriliseds such as 800 times of carbendazim, places 3d and uses.Soil humidity controls at 50-60%
3, domestication before tissue cultured seedling is transplanted
(1) close bottle domestication: when tissue cultured seedling grows to meet seedling exercising requirement, tissue culture bottle is moved on to group training outdoor ventilation and shelters from heat or light place
Closing a bottle seedling exercising, intensity of illumination controls at 1000-2000lux, and temperature, at 20-28 DEG C, is put into outdoor about 2d, then is transported to booth and puts
Putting about 3d, booth intensity of illumination controls at 2000-3000lux, and whole process progressively strengthens illumination, it is to avoid high light directly shines or prominent
So strengthen, observe growth of seedling situation at any time.
(2) open domestication: after closing bottle domestication, bottleneck lid progressively opened and carries out corkage domestication, i.e. first pine lid 2d,
Then part is uncapped 1d, and last uncapping completely places 1-2d, and booth illumination controls at 2000-3000lux, temperature at 20-28 DEG C,
Air humidity controls more than 70%.Taming successful standard is that tissue cultured seedling grows up, and stem and leaf color burn, root system is more sent out
Reaching, seedling is the most healthy and the strongest tall and straight, just transplants after having tamed.
4, the transplanting of tissue cultured seedling
(1) seedling exercising is preferably carried out in the 4-8 month, transplants and is preferably selected in the afternoon or carries out at dusk, and during transplanting, seedbed air humidity should
Controlling more than 70%, temperature controls at 20-28 DEG C, and intensity of illumination controls at 2000-3000lux.
(2) to beat easily when taking out seedling from bottle and put down gently, be careful not to lose root system and stem and leaf.With tweezers, seedling is taken out
Put into the culture medium cleaning root attachment in clear water, then tissue cultured seedling root is put into immersion 5min in 800 times of carbendazols.
(3) according to line-spacing 6cm, spacing in the rows 5cm is transplanted, and first digs the deep duck eye of 2-4cm, be then placed in tissue cultured seedling during transplanting,
Unfolding root system, the degree of depth is advisable so that blade not contacting substrate and root-stem transition zone are equal with substrate face.Transplant while covering base
Matter, is compacted the most gently, sprays water, and injection flow rate wants suitable.1000 times of carbendazim medicinal liquids are sprayed after transplanting.
5, seedbed management
(1) humidity
The present invention ensures air humidity by the method taking Small plastic shed on seedbed, ensures that air humidity is after transplanting in 7d
8-14d humid control is at 80-85%, 15-20d humid control at 70-80%, and medium of seedling bed humid control is at 50-60%.At any time
Observe growth of seedling situation, after having observed that new root length goes out, gradually reduce air humidity, adapt to booth humidity.Observe at any time
Soil humidity, controls the irrigation amount of whole process well.
(2) temperature
Nursery bed temperature is allowed to be maintained at 22-30 DEG C by sheltering from heat or light, ventilate and regulate the measures such as humidity.
(3) illumination
Transplant illumination in 7d and control at 2000-3000lux, 8-14d be 3000-4000lux, 15-20d be 4000-
5000lux, 25-34d be 5000-7000lux, 34-40d be 7000-9000lux.
(4) apply fertilizer
After transplanting after transplanting 7d, spray 0.1% carbamide, after 5d, spray 0.1% dipotassium hydrogen phosphate, interval 5d alternately spray
Water with 0.2% compound fertilizer's aqueous solution interval 7d after executing 0.2% carbamide or dipotassium hydrogen phosphate, about 20d.
(5) the prevention and control of plant diseases, pest control
Pest and disease damage is to put prevention first.After tissue cultured seedling transplants 1 week, every 7d sprays a sterilization such as carbendazim or Mancozeb
Agent.Observe seedling at any time to encroach on or without disease pest, process in time.Remove seedbed undergrowth, dead tissue cultured seedling and weeds, keep
Seedbed neat and tidy.
It is 60% through statistics Folium Callicarpae Formosanae training tissue culture seedling survival rate.
(3) little transplantation of seedlings and management are survived
1, Folium Callicarpae Formosanae survives the standard of little transplantation of seedlings
Plant height reaches more than 15cm, and root growth is flourishing, and leaf color is dark green, and stem is sturdy, well-grown.
2, the management behind lower ground
Transplanting should be selected in overcast and rainy, suitably shelters from heat or light after transplanting in 10d, it is to avoid high light directly shines, and carries out pest management, in time
Water and hoe up weeds.Can apply fertilizer after transplanting 10d, when fertilization principle is for growing vigorous, execute N P and K and other elements more, enter
Branch hardening period, phosphorus was auxiliary based on potassium, promoted children's stem lignifying, in order to survive the winter.
Through statistics lower ground, survival rate is 88%.
Comparative example 1 is from the difference of embodiment 1: the mode keeping seedbed humidity is different, uses full-automatic spraying system
Controlling humidity and can effectively adjust seedbed humidity, Folium Callicarpae Formosanae tissue cultured seedling survival rate is higher.
Comparative example 2
(1) hot house and the requirement in seedbed
1, hot house should possess inside and outside sunshade net, fan and pad, automatic spraying system and control system.Have below seedbed
Drainage system, about the width 1-1.2 rice of seedbed, convenient operation.
(2) tissue cultured seedling seedbed seedling exercising
1, seedling exercising tissue cultured seedling prescription
Plant height reaches 6-8cm, more than 5, blade, and more than root length 3cm, radical more than 5, well developed root system, leaf color is dark green, children
Seedling stalwartness is tall and straight.Get rid of the undesirable tissue cultured seedlinies such as thin and weak Seedling, etiolated seedling, aging Seedling and deformity Seedling.
2, the selection of seedling exercising substrate and process
Selecting Vermiculitum and perlite to mix as seedling exercising substrate according to volume ratio 1:1 ratio, be laid on seedbed, thickness is 12-
14cm, with antibacterial sufficiently steriliseds such as 800 times of carbendazim, places 3d and uses.Soil humidity controls at 50-60%
3, domestication before tissue cultured seedling is transplanted
(1) close bottle domestication: when tissue cultured seedling grows to meet seedling exercising requirement, tissue culture bottle is moved on to group training outdoor ventilation and shelters from heat or light place
Closing a bottle seedling exercising, intensity of illumination controls at 1000-2000lux, and temperature, at 20-28 DEG C, is put into outdoor about 2d, then is transported to booth and puts
Putting about 3d, booth intensity of illumination controls at 2000-3000lux, and whole process progressively strengthens illumination, it is to avoid high light directly shines or prominent
So strengthen, observe growth of seedling situation at any time.
(2) open domestication: after closing bottle domestication, bottleneck lid progressively opened and carries out corkage domestication, i.e. first pine lid 2d,
Then part is uncapped 1d, and last uncapping completely places 1-2d, and booth illumination controls at 2000-3000lux, temperature at 20-28 DEG C,
Air humidity controls more than 70%.Taming successful standard is that tissue cultured seedling grows up, and stem and leaf color burn, root system is more sent out
Reaching, seedling is the most healthy and the strongest tall and straight, just transplants after having tamed.
4, the transplanting of tissue cultured seedling
(1) seedling exercising is preferably carried out in the 4-8 month, transplants and is preferably selected in the afternoon or carries out at dusk, and during transplanting, seedbed air humidity should
Controlling more than 70%, temperature controls at 20-28 DEG C, and intensity of illumination controls at 2000-3000lux.
(2) to beat easily when taking out seedling from bottle and put down gently, be careful not to lose root system and stem and leaf.With tweezers, seedling is taken out
Put into the culture medium cleaning root attachment in clear water, then tissue cultured seedling root is put into immersion 5min in 800 times of carbendazols.
(3) according to line-spacing 6cm, spacing in the rows 5cm is transplanted, and first digs the deep duck eye of 2-4cm, be then placed in tissue cultured seedling during transplanting,
Unfolding root system, the degree of depth is advisable so that blade not contacting substrate and root-stem transition zone are equal with substrate face.Transplant while covering base
Matter, is compacted the most gently, sprays water, and injection flow rate wants suitable.1000 times of carbendazim medicinal liquids are sprayed after transplanting.
5, seedbed management
(1) humidity
The present invention ensures air humidity by the method taking Small plastic shed on seedbed, ensures that air humidity is after transplanting in 7d
8-14d humid control is at 80-85%, 15-20d humid control at 70-80%, and medium of seedling bed humid control is at 50-60%.At any time
Observe growth of seedling situation, after having observed that new root length goes out, gradually reduce air humidity, adapt to booth humidity.Observe at any time
Soil humidity, controls the irrigation amount of whole process well.
(2) temperature
Nursery bed temperature is allowed to be maintained at 22-30 DEG C by sheltering from heat or light, ventilate and regulate the measures such as humidity.
(3) illumination
Transplant illumination in 7d and control at 2000-3000lux, 8-14d be 3000-4000lux, 15-20d be 4000-
5000lux, 25-34d be 5000-7000lux, 34-40d be 7000-9000lux.
(4) apply fertilizer
After transplanting after transplanting 7d, spray 0.1% carbamide, after 5d, spray 0.1% dipotassium hydrogen phosphate, interval 5d alternately spray
Water with 0.2% compound fertilizer's aqueous solution interval 7d after executing 0.2% carbamide or dipotassium hydrogen phosphate, about 20d.
(5) the prevention and control of plant diseases, pest control
Pest and disease damage is to put prevention first.After tissue cultured seedling transplants 1 week, every 7d sprays a sterilization such as carbendazim or Mancozeb
Agent.Observe seedling at any time to encroach on or without disease pest, process in time.Remove seedbed undergrowth, dead tissue cultured seedling and weeds, keep
Seedbed neat and tidy.
It is 70% through statistics Folium Callicarpae Formosanae training tissue culture seedling survival rate.
(3) little transplantation of seedlings and management are survived
1, Folium Callicarpae Formosanae survives the standard of little transplantation of seedlings
Plant height reaches more than 15cm, and root growth is flourishing, and leaf color is dark green, and stem is sturdy, well-grown.
2, the management behind lower ground
Transplanting should be selected in overcast and rainy, suitably shelters from heat or light after transplanting in 10d, it is to avoid high light directly shines, and carries out pest management, in time
Water and hoe up weeds.Can apply fertilizer after transplanting 10d, when fertilization principle is for growing vigorous, execute N P and K and other elements more, enter
Branch hardening period, phosphorus was auxiliary based on potassium, promoted children's stem lignifying, in order to survive the winter.
Through statistics lower ground, survival rate is 91%.
Comparative example 2 is with the difference of embodiment 1: the substrate of seedling exercising is different, and fertile soil and perlite are according to volume ratio
1:1 mixing ratio Vermiculitum and perlite to be got well according to volume ratio 1:1 effect.
Comparative example 3
(1) hot house and the requirement in seedbed
1, hot house should possess inside and outside sunshade net, fan and pad, automatic spraying system and control system.Have below seedbed
Drainage system, about the width 1-1.2 rice of seedbed, convenient operation.
(2) tissue cultured seedling seedbed seedling exercising
1, seedling exercising tissue cultured seedling prescription
Below plant height 6cm, radical less than 5, leaf color is dark green, and seedling stalwartness is tall and straight.Get rid of thin and weak Seedling, etiolated seedling, aging
The undesirable tissue cultured seedlinies such as Seedling and deformity Seedling.
2, the selection of seedling exercising substrate and process
Selecting fertile soil and perlite to mix as seedling exercising substrate according to volume ratio 1:1 ratio, be laid on seedbed, thickness is
12-14cm, with antibacterial sufficiently steriliseds such as 800 times of carbendazim, places 3d and uses.Soil humidity controls at 50-60%
3, domestication before tissue cultured seedling is transplanted
(1) close bottle domestication: when tissue cultured seedling grows to meet seedling exercising requirement, tissue culture bottle is moved on to group training outdoor ventilation and shelters from heat or light place
Closing a bottle seedling exercising, intensity of illumination controls at 1000-2000lux, and temperature, at 20-28 DEG C, is put into outdoor about 2d, then is transported to booth and puts
Putting about 3d, booth intensity of illumination controls at 2000-3000lux, and whole process progressively strengthens illumination, it is to avoid high light directly shines or prominent
So strengthen, observe growth of seedling situation at any time.
(2) open domestication: after closing bottle domestication, bottleneck lid progressively opened and carries out corkage domestication, i.e. first pine lid 2d,
Then part is uncapped 1d, and last uncapping completely places 1-2d, and booth illumination controls at 2000-3000lux, temperature at 20-28 DEG C,
Air humidity controls more than 70%.Taming successful standard is that tissue cultured seedling grows up, and stem and leaf color burn, root system is more sent out
Reaching, seedling is the most healthy and the strongest tall and straight, just transplants after having tamed.
4, the transplanting of tissue cultured seedling
(1) seedling exercising is preferably carried out in the 4-8 month, transplants and is preferably selected in the afternoon or carries out at dusk, and during transplanting, seedbed air humidity should
Controlling more than 70%, temperature controls at 20-28 DEG C, and intensity of illumination controls at 2000-3000lux.
(2) to beat easily when taking out seedling from bottle and put down gently, be careful not to lose root system and stem and leaf.With tweezers, seedling is taken out
Put into the culture medium cleaning root attachment in clear water, then tissue cultured seedling root is put into immersion 5min in 800 times of carbendazols.
(3) according to line-spacing 6cm, spacing in the rows 5cm is transplanted, and first digs the deep duck eye of 2-4cm, be then placed in tissue cultured seedling during transplanting,
Unfolding root system, the degree of depth is advisable so that blade not contacting substrate and root-stem transition zone are equal with substrate face.Transplant while covering base
Matter, is compacted the most gently, sprays water, and injection flow rate wants suitable.1000 times of carbendazim medicinal liquids are sprayed after transplanting.
5, seedbed management
(1) humidity
The present invention ensures air humidity by full-automatic spraying system, ensures that air humidity is that 8-14d is wet after transplanting in 7d
Degree controls at 80-85%, and 15-20d humid control is at 70-80%, and medium of seedling bed humid control is at 50-60%.Observe children at any time
Seedling growing state, after having observed that new root length goes out, gradually reduces air humidity, adapts to booth humidity.Observe substrate at any time wet
Degree, controls the irrigation amount of whole process well.
(2) temperature
Nursery bed temperature is allowed to be maintained at 22-30 DEG C by sheltering from heat or light, ventilate and regulate the measures such as humidity.
(3) illumination
Transplant illumination in 7d and control at 2000-3000lux, 8-14d be 3000-4000lux, 15-20d be 4000-
5000lux, 25-34d be 5000-7000lux, 34-40d be 7000-9000lux.
(4) apply fertilizer
After transplanting after transplanting 7d, spray 0.1% carbamide, after 5d, spray 0.1% dipotassium hydrogen phosphate, interval 5d alternately spray
Water with 0.2% compound fertilizer's aqueous solution interval 7d after executing 0.2% carbamide or dipotassium hydrogen phosphate, about 20d.
(5) the prevention and control of plant diseases, pest control
Pest and disease damage is to put prevention first.After tissue cultured seedling transplants 1 week, every 7d sprays a sterilization such as carbendazim or Mancozeb
Agent.Observe seedling at any time to encroach on or without disease pest, process in time.Remove seedbed undergrowth, dead tissue cultured seedling and weeds, keep
Seedbed neat and tidy.
It is 60% through statistics Folium Callicarpae Formosanae training tissue culture seedling survival rate.
(3) little transplantation of seedlings and management are survived
1, Folium Callicarpae Formosanae survives the standard of little transplantation of seedlings
Plant height reaches more than 15cm, and root growth is flourishing, and leaf color is dark green, and stem is sturdy, well-grown.
2, the management behind lower ground
Transplanting should be selected in overcast and rainy, suitably shelters from heat or light after transplanting in 10d, it is to avoid high light directly shines, and carries out pest management, in time
Water and hoe up weeds.Can apply fertilizer after transplanting 10d, when fertilization principle is for growing vigorous, execute N P and K and other elements more, enter
Branch hardening period, phosphorus was auxiliary based on potassium, promoted children's stem lignifying, in order to survive the winter.
Through statistics lower ground, survival rate is 86%.
Comparative example 3 is with the difference of embodiment 1: the prescription of tissue cultured seedling is different, and plant height reaches 6-8cm, 5, blade
Above, more than root length 3cm, more than 5 survival rates of radical are higher.
Although, the present invention is described in detail the most with a general description of the specific embodiments, but
On the basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to the scope of protection of present invention.
Claims (10)
1. the method for a Folium Callicarpae Formosanae training tissue culture seedling, it is characterised in that comprise the steps:
S1, selection plant height 6～8cm, blade >=5 slice, root length >=3cm, the Folium Callicarpae Formosanae tissue cultured seedling bottle Seedling of radical >=5 piece；
S2, close bottle domestication:
S21, tissue cultured seedling bottle Seedling is moved to the outdoor placement 2d of intensity of illumination 1000～2000lux, temperature 20～28 DEG C；
S22, again tissue cultured seedling bottle Seedling is moved to intensity of illumination 2000～3000lux, temperature 20～28 DEG C booth place 3d；
S3, corkage domestication:
Pine lid 2d, then partly uncap 1d, and last uncapping completely places 1～2d, and booth illumination controls 2000～3000lux,
Temperature controls at 20～28 DEG C, and air humidity controls more than 70%.
Method the most according to claim 1, it is characterised in that in S21, moves to intensity of illumination by tissue cultured seedling bottle Seedling
1800lux, daylight temperature 25～28 DEG C, nocturnal temperature 20～the outdoor of 22 DEG C.
Method the most according to claim 1, it is characterised in that in S22, moves to intensity of illumination by tissue cultured seedling bottle Seedling
2500lux, daylight temperature 25～28 DEG C, nocturnal temperature 20～the booth of 22 DEG C.
Method the most according to claim 1, it is characterised in that in S3, booth illumination controls at 2500lux, daylight temperature
Controlling at 25～28 DEG C, nocturnal temperature controls at 20～22 DEG C.
5. according to the method described in any one of Claims 1 to 4, it is characterised in that described method also comprises the steps:
S4, the tissue cultured seedling completing corkage domestication is cleaned up after be transplanted to seedling exercising in seedbed, seedling exercising substrate is equal-volume than mixed
The fertile soil closed and perlite, seedling exercising soil humidity controls 50～60%；
S5, the seedling of the plant height >=15cm survived by seedling exercising are transplanted in cloth Seedling bag, and transplanting medium is by fertile soil, Vermiculitum and treasure
Zhu Yan mixes according to volume ratio 2:1:1, and transplanting medium humid control is 50～60%.
Method the most according to claim 5, it is characterised in that in S4, during transplanting seedbed air humidity control 70% with
On, air temperature control is at 20～28 DEG C, and intensity of illumination controls 2000～3000lux；Air humidity is controlled in 7d after transplanting
Being 85～95%, illumination controls 2000～3000lux；After transplanting, 8～14d control air humiditys are 80～85%, illumination control
System is 3000～4000lux；After transplanting, 15～20d to control air humiditys be 70～80%, illumination control 4000～
5000lux；After transplanting, 25～34d illumination control 5000～7000lux, and humidity is natural humidity；35～40d illumination after transplanting
Controlling 7000～9000lux, humidity is natural humidity；Nursery bed temperature is maintained at 22～30 DEG C.
Method the most according to claim 6, it is characterised in that S4 comprises the steps:
S41, clean up tissue cultured seedling root, in 700～900 times of carbendazols of dilution, soak 4～6min；
S42, according to line-spacing 6cm, spacing in the rows 5cm is transplanted, and first digs 2～4cm deep duck eyes, be then placed in tissue cultured seedling during transplanting,
Unfolding root system, the degree of depth is advisable so that blade not contacting substrate and root-stem transition zone are equal with substrate face, waters, then spray after transplanting
Dilute 1000 times of carbendazols.
8. according to the method described in claim 6 or 7, it is characterised in that after transplanting 7d, spray 0.1% carbamide and sterilization
Agent, sprays 0.1% dipotassium hydrogen phosphate, alternately sprays 0.2% carbamide and 0.2% dipotassium hydrogen phosphate every 5d, after 20d later after 5d
Water with 0.2% compound fertilizer's aqueous solution interval 7d.
Method the most according to claim 5, it is characterised in that in S5: select that draining is good, soil water-retaining and do not harden,
There is no the ground of weeds as transplanting ground, suitably shelter from heat or light in 10d after transplanting, it is to avoid high light directly shines, carry out pest management, water in time
Water and hoeing up weeds.
Method the most according to claim 9, it is characterised in that can apply fertilizer after transplanting 10d, fertilization principle is growth
Executing N P and K and other elements time vigorous, entrance branch hardening period, phosphorus was auxiliary based on potassium more.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610716690.XA CN106305106A (en) | 2016-08-24 | 2016-08-24 | Method for tissue-culture seedling exercising of callicarpa bodinieri |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610716690.XA CN106305106A (en) | 2016-08-24 | 2016-08-24 | Method for tissue-culture seedling exercising of callicarpa bodinieri |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106305106A true CN106305106A (en) | 2017-01-11 |
Family
ID=57790359
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610716690.XA Pending CN106305106A (en) | 2016-08-24 | 2016-08-24 | Method for tissue-culture seedling exercising of callicarpa bodinieri |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106305106A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108770623A (en) * | 2018-06-23 | 2018-11-09 | 云南省农业科学院农业环境资源研究所 | The overwintering no suspend mode hardening off method of bletilla tissue culture seedlings duplicature |
| CN109496657A (en) * | 2018-12-10 | 2019-03-22 | 怀化正好制药有限公司 | The method of callicarpa kwangtungensis Chun nursery |
| CN113455319A (en) * | 2021-06-21 | 2021-10-01 | 百色学院 | Transplanting method of cassava tissue culture contaminated seedlings |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102668988A (en) * | 2012-06-08 | 2012-09-19 | 江苏九久环境科技有限公司 | Fast seedling breeding method for tissue cultivation of callicarpa bodinieri and method for transplanting rooting seedlings of callicarpa bodinieri |
| CN102870678A (en) * | 2012-10-16 | 2013-01-16 | 海南省农业科学院粮食作物研究所 | Tissue culture rapid propagation method of callicarpa nudiflora |
| CN104221860A (en) * | 2014-09-04 | 2014-12-24 | 四川胜泽源农业集团有限公司 | Rapid propagation method for tissue culture of folium callicarpae pedunculalae |
| CN104663191A (en) * | 2014-09-24 | 2015-06-03 | 江西井冈山天朴药物研究院有限公司 | Method for cultivating callicarpa nudiflora |
| CN105519421A (en) * | 2016-01-11 | 2016-04-27 | 四川七彩林业开发有限公司 | Red-leaf pistacia tissue culture seedling hardening media and seedling hardening method |
-
2016
- 2016-08-24 CN CN201610716690.XA patent/CN106305106A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102668988A (en) * | 2012-06-08 | 2012-09-19 | 江苏九久环境科技有限公司 | Fast seedling breeding method for tissue cultivation of callicarpa bodinieri and method for transplanting rooting seedlings of callicarpa bodinieri |
| CN102870678A (en) * | 2012-10-16 | 2013-01-16 | 海南省农业科学院粮食作物研究所 | Tissue culture rapid propagation method of callicarpa nudiflora |
| CN104221860A (en) * | 2014-09-04 | 2014-12-24 | 四川胜泽源农业集团有限公司 | Rapid propagation method for tissue culture of folium callicarpae pedunculalae |
| CN104663191A (en) * | 2014-09-24 | 2015-06-03 | 江西井冈山天朴药物研究院有限公司 | Method for cultivating callicarpa nudiflora |
| CN105519421A (en) * | 2016-01-11 | 2016-04-27 | 四川七彩林业开发有限公司 | Red-leaf pistacia tissue culture seedling hardening media and seedling hardening method |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108770623A (en) * | 2018-06-23 | 2018-11-09 | 云南省农业科学院农业环境资源研究所 | The overwintering no suspend mode hardening off method of bletilla tissue culture seedlings duplicature |
| CN109496657A (en) * | 2018-12-10 | 2019-03-22 | 怀化正好制药有限公司 | The method of callicarpa kwangtungensis Chun nursery |
| CN109496657B (en) * | 2018-12-10 | 2021-04-06 | 怀化正好制药有限公司 | Callicarpa kwangtungensis seedling culture method |
| CN113455319A (en) * | 2021-06-21 | 2021-10-01 | 百色学院 | Transplanting method of cassava tissue culture contaminated seedlings |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104521579B (en) | Cherry dense and dwarf planting method | |
| CN106106004B (en) | A kind of rapid cuttage propagation method of crape myrtle single plant germplasm | |
| CN100415077C (en) | Cuttage breeding method for photinia frasery | |
| CN105409564B (en) | A kind of cottage method of tea shoot | |
| CN105519421B (en) | A kind of red autumnal leaves Chinese pistache training tissue culture seedling matrix and its hardening off method | |
| CN102511258B (en) | Container seedling-raising method for greenhouse of Chinese wolfberry | |
| CN105557362B (en) | A kind of preparation method of sweet potato potted landscape | |
| CN105309207A (en) | Colorful rose and Chinese rose bonsai cultivation method | |
| CN105940941A (en) | Watermelon planting management method | |
| CN103340070A (en) | Standardized planting technology of polygonatum | |
| CN104303921A (en) | Cultivation method of organic potted blueberry | |
| CN105875128A (en) | Planting method of Chinese prickly ash | |
| CN106342517A (en) | Melaleuca bracteata tissue culture seedling hardening method | |
| CN102172190A (en) | Cultivation method of apple bonsai realizing current-year fruiting and market-selling in Spring Festival | |
| CN104855090A (en) | Cultivation method for autumn flowering peony | |
| CN109275556A (en) | A kind of implantation methods of carnation potted flower | |
| CN104969747A (en) | Method for rapidly cultivating spindle trees indoors | |
| CN1245065C (en) | Potted loquat and its cultivation method | |
| CN105794584A (en) | Potted fruit tree, soft-seed pomegranate and chrysanthemum three-crop interplanting method in greenhouse planting | |
| CN105766299B (en) | Ripe cultural method during a kind of potting large cherry Spring Festival | |
| CN107409670A (en) | A kind of tobacco floating seed rearing pulls out method for transplanting under plant film | |
| CN106258592A (en) | A kind of grafting-cuttage propagation method of Chinese rose | |
| CN105325211A (en) | Method for preparing high-quality late-autumn pineapple bonsai | |
| CN104798682A (en) | Breeding and cultivation method for bitter gourds | |
| CN106305106A (en) | Method for tissue-culture seedling exercising of callicarpa bodinieri |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: 636600 Tissue Culture Workshop Building of Rare and Colorful Leaf Plant Varieties in Guangwu Mountain, Sanshe, Changtan Village, Zhengzheng Town, Nanjiang County, Bazhong City, Sichuan Province Applicant after: Sichuan Qicai Forestry Co., Ltd. Address before: 621000 Huaishu Village Group 6, Dongyu Town, Nanjiang County, Bazhong City, Sichuan Province Applicant before: SICHUAN QICAI FORESTRY INDUSTRY DEVELOPMENT CO., LTD. |
|
| CB02 | Change of applicant information | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170111 |
|
| RJ01 | Rejection of invention patent application after publication |