CN103733958A - Method for transplanting tissue culture seedlings of dendrobium officinale - Google Patents
Method for transplanting tissue culture seedlings of dendrobium officinale Download PDFInfo
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- CN103733958A CN103733958A CN201310691007.8A CN201310691007A CN103733958A CN 103733958 A CN103733958 A CN 103733958A CN 201310691007 A CN201310691007 A CN 201310691007A CN 103733958 A CN103733958 A CN 103733958A
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Abstract
The invention discloses a method for transplanting tissue culture seedlings of dendrobium officinale. The method includes the steps that the tissue culture seedlings of dendrobium officinale are taken out, rinsed, disinfected and then transplanted to a soiless substrate, three to four days after the tissue culture seedlings are field-planted, a vitality element and a nutritional agent are sprayed to leaf surfaces in turn within a month respectively for eight to ten times, the vitality element or the nutritional agent is sprayed every day, and then conventional plantation management is executed. The method for transplanting the tissue culture seedlings of dendrobium officinale has the advantages that the vitality element and the nutritional agent are sprayed to the leaf surfaces of the tissue culture seedlings in turn, vitality of the seedlings is enhanced, the growth vigor of the seedlings is vigorous and robust, leaf color is strong green and glossy, the average height of the seedlings is 15cm after the seedlings are transplanted for half a year, and the average thickness of rootstocks is 0.5cm; disease resistance of the seedlings is improved, and the seedlings are barely diseased; the transplanting survival rate is greatly increased and can reach more than 98%.
Description
Technical field
The present invention relates to agricultural planting field, be specifically related to the trasplanting method of candidum tissue culturing seedling.
Background technology
Dendrobium candidum (formal name used at school: Dendrobium officinale Kimura et Migo), have another name called: ribbed hedyotis herb, Yunnan iron sheet.Belong to micro-specific item, the perennial herbaceous plant that grows nonparasitically upon another plant of the orchid family.Stem is upright, cylindrical, long 9-35 centimetre, and thick 2-4 millimeter, sepal and petal yellow green, closely similar, oval shape lanceolar, is about 1.8cm, wide 4-5mm, the florescence 3-6 month.Mainly be distributed in the ground such as Chinese Anhui, Zhejiang, Fujian.Its stem can be used as medicine, and belongs to the yin tonics in tonic, can reinforcing stomach reg fluid, and nourishing Yin and clearing heat.
The transplanting of candidum tissue culturing seedling at present all adopts soilless substrate grafting.Concrete grammar is: the group training seedling of bottle outlet is after rinsing, sterilization, be transplanted in loose (sawdust or broken bark etc.) matrix, by artificial regulatory illumination, moisture content and nutrient, and the extermination of disease and insect pest, make candidum tissue culturing seedling normal growth, this technology is routine and manages and protects method.
The shortcoming of prior art is: a little less than dendrobium candidum seedling growing way, transplant after half a year only 6cm of seedling mean height, the thick 0.3cm of average diameter; Resistance is poor, normal large area morbidity; Transplanting success is low, is only 10-60%.
Summary of the invention
Object of the present invention is exactly for above-mentioned defect of the prior art, provides a kind of transplanting success high, the trasplanting method of the candidum tissue culturing seedling that plant growing way is vigorous.
To achieve these goals, technical scheme provided by the invention is: the trasplanting method of candidum tissue culturing seedling, that the group training seedling of dendrobium candidum is taken out, after rinsing, sterilization, migrate to soilless substrate, after field planting 3-4 days, in one month, blade face is sprayed to Huolisu and nutritional agents each 8-10 time in turn, spray a kind of every day, then proceed to general planting management.
Further, the trasplanting method of above-mentioned candidum tissue culturing seedling, comprises the following steps:
1) candidum tissue culturing seedling is taken out from vial, be placed in clean plastic basin;
2) candidum tissue culturing seedling step 1) being taken out is placed in the clean residual medium of its root of clear water rinsing, obtains clean group training seedling;
3) by step 2) the cleaning group that obtains training seedling is placed in sterilization in plastic basin, and sterilization method is for adopting 1000 times of thiophanate methyl dilute solutions or carbendazim dilute solution to soak 10 minutes, then group training seedling is dried to root and turned white, and after being sterilized, group is trained seedling;
4) after sterilization step 3) being obtained, organize training seedling and transplant on soilless substrate, every 2-3 bud seedling is one clump, and clump spacing specification is 5 * 5cm;
5) until the group training seedling in step 4), migrate on soilless substrate after field planting 3-4 days, since the 5th day every day, group is trained to seedling blade face and spray in turn Huolisu and nutritional agents; The working concentration of medicament is 800-1000 times of dilute solution, and spray a kind of every day, and every kind of medicament sprays 8-10 time, spray moistening to blade face till, after 1 month, can proceed to general planting management.
Proceed to after general planting management, the working concentration of medicament is 4000 times.
Further, the trasplanting method of above-mentioned candidum tissue culturing seedling, the preparation method of described soilless substrate is: the broken bark of the base starting material fully fermenting or sawdust are layered on seedbed, base starting material laying depth is 10-18cm, first with clear water, drench, with 800 times of microbicide solutions, irrigate sterilization again, obtain soilless substrate after being filtered dry the moisture in base starting material.
Further, the trasplanting method of above-mentioned candidum tissue culturing seedling, the fermentation process of the broken bark of described base starting material or sawdust is: broken bark or sawdust are divided on the ground, and the thick 20-30cm of raw material, is sprayed to raw material with the aqueous solution of urea that mass percentage concentration is 0.5% drenched, then bunch, heap height is 1-1.2m, covers thin film stack retting, the turning after month of fermenting, again cover film ferments one month again, obtains the base starting material that becomes thoroughly decomposed.
Beneficial effect of the present invention is: the trasplanting method of candidum tissue culturing seedling provided by the invention, by group training seedling blade face is sprayed to Huolisu and nutritional agents in turn, has reached following effect:
1, plant vigor is strengthened, growing way is vigorous and healthy and strong, and leaf look strong green and glossy is transplanted seedling mean height 15cm after half a year, the thick 0.5cm of average diameter;
2, plant disease resistance improves, seldom morbidity;
3, transplanting success increases substantially, and can reach more than 98%.
Accompanying drawing explanation
Fig. 1 sprays the schematic flow sheet of Huolisu and nutritional agents after the field planting of candidum tissue culturing bottle seedling.
Embodiment
The preparation source of using is:
Huolisu: leaves of plants despot's (Weifang Jian Pai biotechnology research institute) 500 times of dilutions and 500 times of dilution equal-volumes of emerald green skin of bamboo B-1 Huolisu (Guangdong crop diseases and pest observes and predicts Zhan Nong section and serves society) mix.
Nutritional agents preparation: accurately take urea 5.4kg, ammonium sulfate 0.45kg, ammonium dihydrogen phosphate (ADP) 1.25kg, potassium nitrate 1.8kg, potassium dihydrogen phosphate 0.45kg, magnesium sulfate 0.9kg, micro-fertilizer (STEM) 0.1kg.If raw material is bulk or particle, need to pulverize, then various compositions are fully mixed and obtain nutritional agents.
Thiophanate methyl: Yun Fa chemical industry (Shanghai) Co., Ltd..
Carbendazim: agricultural chemicals Co., Ltd of Zibo Hang Seng.
Bactericide (sterilization soilless substrate is used): tpn (Guangdong Zhongxun Agricultural Technology Co., Ltd.).
embodiment 1:
(1) group training seedling bottle outlet: with long handle tweezers, candidum tissue culturing seedling is taken out in vial, be placed in clean plastic basin;
(2) rinsing: group training seedling is placed in to clear water, the medium rinsing that remains in root is clean;
(3) sterilization: a bottle seedling of cleaning is placed in plastic basin, with 1000 times of thiophanate methyls or carbendazim immersion 10 minutes, then pulls In Shade drying to root out and turn white, wait to transplant;
(4) transplant: the broken bark fully fermenting or sawdust are layered on seedbed, stromal thickness 10～18cm, first with clear water, drench, with 800 times of bactericide, irrigate sterilization again, after matrix is filtered dry water, the bottle seedling disinfecting is implanted in matrix, and general 2～3 buds are a little Cong, and the plantation specification between Cong Yucong is 5 * 5cm;
(5) spray Huolisu and nutritional agents: as shown in Figure 1, bottle seedling field planting is in seedbed after 3～4 days, within the 5th day, start every day to foliage-spray Huolisu and nutritional agents, the concentration that these two kinds of medicaments are used is 800～1000 times, within one day, only spray a kind of, spray in turn, every kind of medicament sprays 8～10 times, spray at every turn take blade face moistening as degree, megathermal period autumn in summer, should spray at 9～10 o'clock of the morning, winter in spring, should spray at warm noon, pay special attention to, overcast and rainy or sombre (temperature is lower than 15 ℃) weather should not spray;
(6) Routine Management: tissue-culture container seedling field planting is after spraying Huolisu and nutritional agents, and its growing way is vigorous, survival rate, up to more than 98%, proceeds to Routine Management subsequently, and dendrobium candidum Routine Management is as follows:
1, temperature is controlled: regulate ventilation and the sunshade net of booth, keep temperature of shed in 12-35 ℃, with 25-30 ℃ ideal, guarantee winter more than 0 ℃;
2, illumination control and management: dendrobium candidum is liked half cloudy half light, nice and cool environment, and morning and evening or cloudy day should be opened sunshade net, during fine day high temperature, suitable cover net sunshade;
3, humidity (moisture content) is controlled: dendrobium candidum optimum growth humidity is in 80% left and right, and matrix is micro-to wet as well, if matrix overdrying is unfavorable for Growth of Dendrobium candidum, even can dewater and death; Soil humidity is excessive, can cause rhizome to rot, and dendrobium candidum humidity is controlled experience: air humidity is suitable greatly, root system humidity should be little; Megathermal period autumn in summer, should be in the morning or sprinkling irrigation at dusk, avoid middle afternoon to spray during high temperature; Winter in spring, should be in warm sprinkling irrigation at noon;
4, fertilizing management: temperature is when more than 20 ℃, and fertilising in good time, promotes plant strain growth;
Foliage fertilizer: be chosen in the morning (before 10 o'clock) of sunlight, spray one time foliage fertilizer every 1-2 days, foliage fertilizer is selected the high nitrogen of orchid or on average fertile, 4000 times of working concentrations;
It is fertile that root is filled with: within every 7-10 days, root is filled with once, selects orchid high nitrogen fertilizer or on average fertile spring and summer, selects the average fertilizer of orchid or high phosphorus potash fertilizer autumn, and doubly, sombre weather in winter (15 ℃ following) should not apply fertilizer working concentration 2500-3000; When dendrobium candidum seedling grows tall to 15-20 centimeters, fertilising should be take organic manure as main, suitably joins and executes inorganic fertilizer, can improve quality and improve output;
5, extermination of disease and insect pest principle: as far as possible reduce agricultural chemicals access times and dosage, to reduce the pollution to medicinal material and environment, when necessary applying pesticides, should be in strict accordance with the regulation of the agricultural chemicals management rules > > of the < < People's Republic of China (PRC), select efficient, low toxicity, less-persistent pesticide, ban use of severe toxicity, high residual hazard agricultural chemicals.
Finally it should be noted that: the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although the present invention is had been described in detail with reference to previous embodiment, for a person skilled in the art, its technical scheme that still can record aforementioned each embodiment is modified, or part technical characterictic is wherein equal to replacement.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (4)
1. the trasplanting method of candidum tissue culturing seedling, it is characterized in that, that the group training seedling of dendrobium candidum is taken out, after rinsing, sterilization, migrate to soilless substrate, after field planting 3-4 days, in one month, blade face is sprayed to Huolisu and nutritional agents each 8-10 time in turn, spray a kind of every day, then proceed to general planting management.
2. the trasplanting method of candidum tissue culturing seedling according to claim 1, is characterized in that, comprises the following steps:
1) candidum tissue culturing seedling is taken out from vial, be placed in clean plastic basin;
2) candidum tissue culturing seedling step 1) being taken out is placed in the clean residual medium of its root of clear water rinsing, obtains clean group training seedling;
3) by step 2) the cleaning group that obtains training seedling is placed in sterilization in plastic basin, and sterilization method is for adopting 1000 times of thiophanate methyl dilute solutions or carbendazim dilute solution to soak 10 minutes, then group training seedling is dried to root and turned white, and after being sterilized, group is trained seedling;
4) after sterilization step 3) being obtained, organize training seedling and transplant on soilless substrate, every 2-3 bud seedling is one clump, and clump spacing specification is 5 * 5cm;
5) until the group training seedling in step 4), migrate on soilless substrate after field planting 3-4 days, since the 5th day every day, group is trained to seedling blade face and spray in turn Huolisu and nutritional agents; The working concentration of medicament is 800-1000 times of dilute solution, and spray a kind of every day, and every kind of medicament sprays 8-10 time, spray moistening to blade face till, after 1 month, can proceed to general planting management.
3. the trasplanting method of candidum tissue culturing seedling according to claim 2, it is characterized in that, the preparation method of described soilless substrate is: the broken bark of the base starting material fully fermenting or sawdust are layered on seedbed, base starting material laying depth is 10-18cm, first with clear water, drench, with 800 times of microbicide solutions, irrigate sterilization again, obtain soilless substrate after being filtered dry the moisture in base starting material.
4. the trasplanting method of candidum tissue culturing seedling according to claim 3, it is characterized in that, the fermentation process of the broken bark of described base starting material or sawdust is: broken bark or sawdust are divided on the ground, and the thick 20-30cm of raw material, is sprayed to raw material with the aqueous solution of urea that mass percentage concentration is 0.5% drenched, then bunch, heap height is 1-1.2m, covers thin film stack retting, the turning after month of fermenting, again cover film ferments one month again, obtains the base starting material that becomes thoroughly decomposed.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104686308A (en) * | 2015-02-11 | 2015-06-10 | 贵州绿春缘有机农业产业管理有限公司 | Cultivation method of Dendrobium officinale Kimura et Migo |
| CN104770277A (en) * | 2015-03-11 | 2015-07-15 | 顺德职业技术学院 | Zero-emission dendrobium officinale planting method |
| CN104838998A (en) * | 2015-06-03 | 2015-08-19 | 饶平茂丰源生物科技有限公司 | Under-forest dendrobium candidum soilless stereoscopic planting method |
| CN105000931A (en) * | 2015-07-07 | 2015-10-28 | 大新县科学技术情报研究所 | Dendrobium candidum matrix manufacture method |
| CN107396782A (en) * | 2016-05-19 | 2017-11-28 | 深圳市天之宝生物科技股份有限公司 | A kind of implantation methods prevented and treated the stem of noble dendrobium and be harmful to molluscan method and the stem of noble dendrobium |
| CN108157120A (en) * | 2017-12-27 | 2018-06-15 | 康美药业（文山）药材种植管理有限公司 | A kind of Dendrobium Chrysotoxum Lindl culture substrate and preparation method thereof |
| CN108513986A (en) * | 2018-02-08 | 2018-09-11 | 湛江市东方园艺有限公司 | A kind of fairy bamboo cultivation agent |
| CN111955332A (en) * | 2020-08-25 | 2020-11-20 | 中雁石斛技术股份有限公司 | Planting method of dendrobium officinale |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101142880A (en) * | 2007-10-12 | 2008-03-19 | 云南大学 | All light radiation artificial cultivating method for officinal dendrobium |
| CN101444181A (en) * | 2008-12-19 | 2009-06-03 | 查应洪 | Method for growing dendrobium |
| CN101766113A (en) * | 2010-01-25 | 2010-07-07 | 大连普瑞康生物技术有限公司 | Method for preparing dendrobium officinale culture medium |
| WO2011014663A1 (en) * | 2009-07-29 | 2011-02-03 | Syngenta Participations Ag | Process of improving transplant plants |
| CN102282998A (en) * | 2011-06-28 | 2011-12-21 | 云南红土生源药用生物科技开发有限公司 | Dendrobium offcinale tissue cultured seedling training and domesticating method |
| CN103210846A (en) * | 2013-04-27 | 2013-07-24 | 凌云县长生仙草生物科技开发有限公司 | Method for cultivating dendrobium candidum tissue-culture infected seedlings |
-
2013
- 2013-12-17 CN CN201310691007.8A patent/CN103733958A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101142880A (en) * | 2007-10-12 | 2008-03-19 | 云南大学 | All light radiation artificial cultivating method for officinal dendrobium |
| CN101444181A (en) * | 2008-12-19 | 2009-06-03 | 查应洪 | Method for growing dendrobium |
| WO2011014663A1 (en) * | 2009-07-29 | 2011-02-03 | Syngenta Participations Ag | Process of improving transplant plants |
| CN101766113A (en) * | 2010-01-25 | 2010-07-07 | 大连普瑞康生物技术有限公司 | Method for preparing dendrobium officinale culture medium |
| CN102282998A (en) * | 2011-06-28 | 2011-12-21 | 云南红土生源药用生物科技开发有限公司 | Dendrobium offcinale tissue cultured seedling training and domesticating method |
| CN103210846A (en) * | 2013-04-27 | 2013-07-24 | 凌云县长生仙草生物科技开发有限公司 | Method for cultivating dendrobium candidum tissue-culture infected seedlings |
Non-Patent Citations (2)
| Title |
|---|
| 张真 等编著: "《"浙八味"中药材规范化生产技术》", 31 December 2007, 科学普及出版社 * |
| 杨明志 等主编: "《中国药用石斛标准研究与应用》", 31 December 2012, 四川科学技术出版社 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104686308A (en) * | 2015-02-11 | 2015-06-10 | 贵州绿春缘有机农业产业管理有限公司 | Cultivation method of Dendrobium officinale Kimura et Migo |
| CN104770277A (en) * | 2015-03-11 | 2015-07-15 | 顺德职业技术学院 | Zero-emission dendrobium officinale planting method |
| CN104838998A (en) * | 2015-06-03 | 2015-08-19 | 饶平茂丰源生物科技有限公司 | Under-forest dendrobium candidum soilless stereoscopic planting method |
| CN105000931A (en) * | 2015-07-07 | 2015-10-28 | 大新县科学技术情报研究所 | Dendrobium candidum matrix manufacture method |
| CN107396782A (en) * | 2016-05-19 | 2017-11-28 | 深圳市天之宝生物科技股份有限公司 | A kind of implantation methods prevented and treated the stem of noble dendrobium and be harmful to molluscan method and the stem of noble dendrobium |
| CN108157120A (en) * | 2017-12-27 | 2018-06-15 | 康美药业（文山）药材种植管理有限公司 | A kind of Dendrobium Chrysotoxum Lindl culture substrate and preparation method thereof |
| CN108513986A (en) * | 2018-02-08 | 2018-09-11 | 湛江市东方园艺有限公司 | A kind of fairy bamboo cultivation agent |
| CN111955332A (en) * | 2020-08-25 | 2020-11-20 | 中雁石斛技术股份有限公司 | Planting method of dendrobium officinale |
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