CN104686308A - Cultivation method of Dendrobium officinale Kimura et Migo - Google Patents
Cultivation method of Dendrobium officinale Kimura et Migo Download PDFInfo
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- CN104686308A CN104686308A CN201510070379.8A CN201510070379A CN104686308A CN 104686308 A CN104686308 A CN 104686308A CN 201510070379 A CN201510070379 A CN 201510070379A CN 104686308 A CN104686308 A CN 104686308A
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- 241001076416 Dendrobium tosaense Species 0.000 title abstract description 9
- 238000012364 cultivation method Methods 0.000 title abstract 4
- 239000011159 matrix material Substances 0.000 claims abstract description 16
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 6
- 239000005842 Thiophanate-methyl Substances 0.000 claims abstract description 5
- 238000004140 cleaning Methods 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims abstract description 5
- QGHREAKMXXNCOA-UHFFFAOYSA-N thiophanate-methyl Chemical group COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC QGHREAKMXXNCOA-UHFFFAOYSA-N 0.000 claims abstract description 5
- 238000009423 ventilation Methods 0.000 claims abstract description 5
- 241000026010 Dendrobium candidum Species 0.000 claims description 25
- 238000012258 culturing Methods 0.000 claims description 22
- 235000010204 pine bark Nutrition 0.000 claims description 22
- 238000009395 breeding Methods 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 241000196324 Embryophyta Species 0.000 claims description 8
- 239000003337 fertilizer Substances 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 7
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 7
- 241001330002 Bambuseae Species 0.000 claims description 7
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 7
- 239000011425 bamboo Substances 0.000 claims description 7
- 239000003610 charcoal Substances 0.000 claims description 7
- 239000004575 stone Substances 0.000 claims description 7
- 239000007921 spray Substances 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 4
- 241000255789 Bombyx mori Species 0.000 claims description 4
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 4
- 241001494479 Pecora Species 0.000 claims description 4
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 claims description 4
- 235000018660 ammonium molybdate Nutrition 0.000 claims description 4
- 239000011609 ammonium molybdate Substances 0.000 claims description 4
- 229940010552 ammonium molybdate Drugs 0.000 claims description 4
- 229910021538 borax Inorganic materials 0.000 claims description 4
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims description 4
- 210000003608 fece Anatomy 0.000 claims description 4
- 238000000855 fermentation Methods 0.000 claims description 4
- 230000004151 fermentation Effects 0.000 claims description 4
- 229960001781 ferrous sulfate Drugs 0.000 claims description 4
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 4
- 239000011790 ferrous sulphate Substances 0.000 claims description 4
- 238000011081 inoculation Methods 0.000 claims description 4
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 4
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 4
- 230000002262 irrigation Effects 0.000 claims description 4
- 238000003973 irrigation Methods 0.000 claims description 4
- 229940071125 manganese acetate Drugs 0.000 claims description 4
- UOGMEBQRZBEZQT-UHFFFAOYSA-L manganese(2+);diacetate Chemical compound [Mn+2].CC([O-])=O.CC([O-])=O UOGMEBQRZBEZQT-UHFFFAOYSA-L 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 235000015921 sodium selenite Nutrition 0.000 claims description 4
- 239000011781 sodium selenite Substances 0.000 claims description 4
- 229960001471 sodium selenite Drugs 0.000 claims description 4
- 235000010339 sodium tetraborate Nutrition 0.000 claims description 4
- 239000004328 sodium tetraborate Substances 0.000 claims description 4
- 239000000375 suspending agent Substances 0.000 claims description 4
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 4
- 235000009529 zinc sulphate Nutrition 0.000 claims description 4
- 239000011686 zinc sulphate Substances 0.000 claims description 4
- 238000001035 drying Methods 0.000 abstract 1
- 238000002791 soaking Methods 0.000 abstract 1
- 239000000725 suspension Substances 0.000 abstract 1
- 210000001519 tissue Anatomy 0.000 description 18
- 230000000694 effects Effects 0.000 description 5
- 150000004676 glycans Chemical class 0.000 description 4
- 229920001282 polysaccharide Polymers 0.000 description 4
- 239000005017 polysaccharide Substances 0.000 description 4
- 241000607479 Yersinia pestis Species 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 241001523681 Dendrobium Species 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 241000233855 Orchidaceae Species 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000003014 reinforcing effect Effects 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 208000002177 Cataract Diseases 0.000 description 1
- 206010010144 Completed suicide Diseases 0.000 description 1
- 241000938605 Crocodylia Species 0.000 description 1
- 241000237858 Gastropoda Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 201000007100 Pharyngitis Diseases 0.000 description 1
- 241000256626 Pterygota <winged insects> Species 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 229910000746 Structural steel Inorganic materials 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 208000031971 Yin Deficiency Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000002605 anti-dotal effect Effects 0.000 description 1
- 238000013475 authorization Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 201000001881 impotence Diseases 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 206010036067 polydipsia Diseases 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 230000005760 tumorsuppression Effects 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a cultivation method of Dendrobium officinale Kimura et Migo. The cultivation method comprises a step for planting tissue culture seedlings of Dendrobium officinale Kimura et Migo as follows: taking out 5-7 cm long healthy tissue culture seedlings of Dendrobium officinale Kimura et Migo from a tissue culture flask, cleaning the tissue culture seedlings, placing roots of the tissue culture seedlings of Dendrobium officinale Kimura et Migo into 1000 times liquid of 50% thiophanate methyl suspension for disinfection, soaking the roots for 10 minutes, drying the roots in a shade ventilation place, planting the tissue culture seedlings of Dendrobium officinale Kimura et Migo into a matrix of a seedbed after the roots become white completely. The cultivation method of Dendrobium officinale Kimura et Migo is simple in operation and is conducive to the industrial application at least.
Description
Technical field
The present invention relates to the breeding method of a Plants, particularly relate to a kind of breeding method of dendrobium candidum.
Background technology
Dendrobium candidum (Dendrobium officinale Kimura et Migo) is China's traditional Chinese medicine material, be medicinal plant famous and precious in the orchid family (Orchidaceae) Dendrobium (Dendrobium), be mainly distributed in the ground such as Guizhou, Yunnan, Guangxi, Jiangxi, Anhui and Hunan.Record according to Compendium of Material Medica, dendrobium candidum has " cure mainly in wound, tonifying five zang organs consumptive disease, reinforcing yin essence benefit essence, mend in never sufficient, flat stomach Qi, the intelligence development of longue meat is except shying, making light of one's life by commiting suicide effect effect of prolonging life ".The Pharmacopoeia of the People's Republic of China according to version in 2010 is recorded, dendrobium candidum has reinforcing stomach reg fluid and the effect such as nourishing Yin and clearing heat, be usually used in pyreticosis Tianjin wound, dry polydipsia, deficiency of stomach-Yin, food retched less, after being ill abnormal heat do not move back, the treatment of fire excess from yin deficiency, hectic fever due to yin labor heat, the order diseases such as secretly not clear and muscles and bones impotence is soft.Its stem is used as medicine, and all having remarkable result to treatment pharyngolaryngitis, raising body immunity and even Tumor suppression, is the health-care good product had won fame both at home and abroad.
Dendrobium candidum requires strict to growing environment, mostly be distributed in the cool high humidity area of temperature of height above sea level 250-900 rice, affect larger by climatic factors such as the relative moisture in subenvironment, temperature, illumination, owing to needing to have little understanding to its growing environment, artificial cultivation is comparatively difficult, mainly rely on wild resource for a long time, current dendrobium officinale resource is endangered.Dendrobium candidum is protection species and China second class protection endangered plants of " wild plant international trade pact in imminent danger ", is embargo in the world.At present the research of dendrobium candidum water-soluble polysaccharide aspect is mainly comprised to the Structural Identification of the regularity of distribution of polysaccharide in Different Organs, extracting and developing, purifying and key component, and the aspect such as pharmacological effect, result of study shows that the polyoses content of dendrobium candidum under different planting can be variant.
Modern medicine study proves, the polysaccharide in dendrobium candidum can significantly improve human body SOD level, reduces LPO level, can promote that T cell grow, thus plays and strengthen immune and antidotal effect, has significant effect to tonic after old man, cancer patient chemicotherapy.Good, definite curative effect is all had in treatment diabetes, cataract, chronic pharyngitis etc.Authorization Notice No. be CN102172152B patent utilizes illumination, temperature simultaneously, fertilizer sprays, water waters, sound stimulation, and just sprouted time control flower bud in conjunction with bud and cultivation management is carried out to the method for flowering parts manual removal bud, result shows the content that the method can improve polysaccharide in dendrobium candidum, but the method complicated operation, is unfavorable for industrialization production application.
Therefore, simple to operate desirable to provide one, be beneficial to the breeding method of the dendrobium candidum of commercial application.
Summary of the invention
For this reason, the present invention proposes a kind of breeding method of the novel dendrobium candidum at least partially that can solve the problem.
According to an aspect of the present invention, provide a kind of breeding method of dendrobium candidum, comprise
Candidum tissue culturing seedling field planting step: the healthy and strong candidum tissue culturing seedling of long 5-7cm is taken out from tissue culture bottle, after cleaning, the root of described candidum tissue culturing seedling is put into 1000 times of liquid 50% thiophanate-methyl suspending agents carry out disinfection, soak after 10 minutes, dry in the air in ventilation of sheltering from heat or light and put, plant in the matrix in seedbed after the coring color of described candidum tissue culturing seedling bleaches completely.
Alternatively, according to an embodiment of the invention, after described candidum tissue culturing seedling field planting step, comprise the management process after field planting further:
A week is kept not spray water, until the root end of described candidum tissue culturing seedling just starts sprinkling irrigation after starting growth, keep the humid control of booth at 60%-80%, its temperature controls at 22-30 DEG C daytime, night is according to natural temperature, and winter nights minimum temperature remains on subzero more than 3 DEG C.
Alternatively, according to an embodiment of the invention, described matrix comprises stone, pine bark, pine bark and bamboo charcoal,
Wherein, the volume ratio of each component is stone: pine bark: pine bark: bamboo charcoal=1:6:1:2.
Alternatively, according to an embodiment of the invention, the bottom of described matrix is provided with the pine bark that thickness is 13-15 centimetre, and the surface of described matrix is provided with the pine bark that thickness is 1-2cm.
Alternatively, according to an embodiment of the invention, comprise fertilising step further: annual March, August respectively apply a fertilizer to described candidum tissue culturing seedling.
Alternatively, according to an embodiment of the invention, described fertilizer is mixed with oil cake, sheep excrement, rabbit excrement, silkworm faeces borax, sodium selenite, ammonium molybdate, manganese acetate, ferrous sulfate, zinc sulphate, mixes suitable quantity of water, and the loading of inoculation biological bacteria obtains for fermenting tank for fermentation 3-4 month.
The breeding method of dendrobium candidum provided by the invention is at least simple to operate, is conducive to commercial application.
Embodiment
The invention provides many applicable creative concepts, this creative concept can be reflected in a large number of in concrete context.The specific embodiment described in following embodiments of the present invention only as the exemplary illustration of the specific embodiment of the present invention, and does not form limitation of the scope of the invention.
Below in conjunction with concrete embodiment, the invention will be further described.
A kind of breeding method of dendrobium candidum, comprise candidum tissue culturing seedling field planting step: taken out from tissue culture bottle by the healthy and strong candidum tissue culturing seedling of long 5-7cm, after cleaning, the root of described candidum tissue culturing seedling is put into 1000 times of liquid 50% thiophanate-methyl suspending agents carry out disinfection, soak after 10 minutes, dry in the air in ventilation of sheltering from heat or light and put, plant in the matrix in seedbed after the coring color of described candidum tissue culturing seedling bleaches completely.Described matrix comprises stone, pine bark, pine bark and bamboo charcoal, and wherein, the volume ratio of each component is stone: pine bark: pine bark: bamboo charcoal=1:6:1:2.The bottom of described matrix is provided with the pine bark that thickness is 13-15 centimetre, and the surface of described matrix is provided with the pine bark that thickness is 1-2cm.
Management process after comprising field planting further after described candidum tissue culturing seedling field planting step: keep a week not spray water, until the root end of described candidum tissue culturing seedling just starts sprinkling irrigation after starting growth, keep the humid control of booth at 60%-80%, its temperature controls at 22-30 DEG C daytime, night is according to natural temperature, and winter nights minimum temperature remains on subzero more than 3 DEG C.
The breeding method of described dendrobium candidum comprises fertilising step further: annual March, August respectively apply a fertilizer to described candidum tissue culturing seedling.Described fertilizer is mixed with oil cake, sheep excrement, rabbit excrement, silkworm faeces borax, sodium selenite, ammonium molybdate, manganese acetate, ferrous sulfate, zinc sulphate, mixes suitable quantity of water, and the loading of inoculation biological bacteria obtains for fermenting tank for fermentation 3-4 month.
Further the breeding method of dendrobium candidum provided by the invention is explained in detail below:
Preparing nursery bed: build booth, ceiling and surrounding 12 Anti-fogging Film, ceiling covers the shade net of shade density about 80%.
Seedbed is provided with in canopy
1. the specification in seedbed is that 60cm is high, wide 1.5m, long 28m.
2. seedbed frame is angle iron frame, plastic wire bound edge.
3. matrix: bottom is plugged with the pine bark of 3-5cm specification, high-order 14cm, upper strata is the pine bark of 0.5-0.8cm, and thickness is about 1cm.
The raw material of hypothallus makes and fertilizer uses
The stone of matrix formulations: 2-3cm, the pine bark of 3-5cm, the pine bark of 0.5-0.8cm, the bamboo charcoal of 3-5cm, matrix volume ratio is 1:6:1:2, after 120 DEG C of high-temperature sterilizations, enter use.
Borax, sodium selenite, ammonium molybdate, manganese acetate, ferrous sulfate, zinc sulphate are mixed with oil cake, sheep excrement, rabbit excrement, silkworm faeces, mix suitable quantity of water, inoculation biological bacteria loads fermenting tank for fermentation 3-4 month, and 20 grams every clump, annual March, August respectively execute once.
Field planting: by the taking-up gently from tissue culture bottle of the healthy and strong candidum tissue culturing seedling of long 5-7cm, after cleaning, root is put into 1000 times of liquid 50% thiophanate-methyl suspending agents carry out disinfection, soak after 10 minutes, dry in the air to put in ventilation of sheltering from heat or light and plant in seedbed after coring color bleaches completely.
Management after field planting: seed keeps a week not spray water, etc. root end start growth after just start sprinkling irrigation, keep the humid control of booth at 60%-80%, its temperature controls at 22-30 DEG C daytime, night is according to natural temperature, and winter nights minimum temperature remains on subzero more than 3 DEG C.
The fertilising process of seed
4, diseases and pests controlling
(1) insect pest that dendrobium candidum is maximum is the reptiles such as snail, adopts on every pin in seedbed and places plastic ware, add salt in vessel, stops insect pest to enter in seedbed.
(2) control of winged insect, adopts sky sun can trapping lamp, and booth periphery has specification to be the fly net isolation of mesh 0.03cm.
It should be noted that the present invention and each product, raw material etc. be that prior art provides.Its composition is as without special instruction, and those of ordinary skill in the art all can obtain according to routine techniques means.
It should be noted, the present invention will be described instead of limit the invention for above-described embodiment, and those skilled in the art can design alternative embodiment when not departing from the scope of claims.In the claims, any reference symbol between bracket should be configured to limitations on claims.Word " comprises " not to be got rid of existence and does not arrange element in the claims or step.Word "a" or "an" before being positioned at element is not got rid of and be there is multiple such element.Word first, second and third-class use do not represent any order.Can be title by these word explanations.
Claims (6)
1. a breeding method for dendrobium candidum, comprises
Candidum tissue culturing seedling field planting step: the healthy and strong candidum tissue culturing seedling of long 5-7cm is taken out from tissue culture bottle, after cleaning, the root of described candidum tissue culturing seedling is put into 1000 times of liquid 50% thiophanate-methyl suspending agents carry out disinfection, soak after 10 minutes, dry in the air in ventilation of sheltering from heat or light and put, plant in the matrix in seedbed after the coring color of described candidum tissue culturing seedling bleaches completely.
2. the breeding method of dendrobium candidum according to claim 1, comprises the management process after field planting further after described candidum tissue culturing seedling field planting step:
A week is kept not spray water, until the root end of described candidum tissue culturing seedling just starts sprinkling irrigation after starting growth, keep the humid control of booth at 60%-80%, its temperature controls at 22-30 DEG C daytime, night is according to natural temperature, and winter nights minimum temperature remains on subzero more than 3 DEG C.
3. the breeding method of dendrobium candidum according to claim 2, described matrix comprises stone, pine bark, pine bark and bamboo charcoal,
Wherein, the volume ratio of each component is stone: pine bark: pine bark: bamboo charcoal=1:6:1:2.
4. the breeding method of dendrobium candidum according to claim 2, the bottom of described matrix is provided with the pine bark that thickness is 13-15 centimetre, and the surface of described matrix is provided with the pine bark that thickness is 1-2cm.
5. the breeding method of dendrobium candidum according to claim 1, comprises fertilising step further: annual March, August respectively apply a fertilizer to described candidum tissue culturing seedling.
6. the breeding method of dendrobium candidum according to claim 5, described fertilizer is mixed with oil cake, sheep excrement, rabbit excrement, silkworm faeces borax, sodium selenite, ammonium molybdate, manganese acetate, ferrous sulfate, zinc sulphate, mix suitable quantity of water, the loading of inoculation biological bacteria obtains for fermenting tank for fermentation 3-4 month.
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| CN201510070379.8A CN104686308B (en) | 2015-02-11 | 2015-02-11 | A kind of breeding method of dendrobium candidum |
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| CN201510070379.8A CN104686308B (en) | 2015-02-11 | 2015-02-11 | A kind of breeding method of dendrobium candidum |
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| CN104686308B CN104686308B (en) | 2017-10-10 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105248262A (en) * | 2015-10-08 | 2016-01-20 | 浙江省农业科学院 | Pine bark-biochar matrix for culture of Dendrobium officinale |
| CN106035028A (en) * | 2016-06-30 | 2016-10-26 | 上海孙桥农业科技股份有限公司 | Cultivation substrate for dendrobium officinale and preparation method |
| CN106565323A (en) * | 2016-10-27 | 2017-04-19 | 安徽省霍山县祥佑堂中药材开发有限公司 | Special liquid fertilizer for dendrobe in field planting period |
| CN106942031A (en) * | 2016-12-30 | 2017-07-14 | 浙江金石生物科技有限公司 | A kind of high-quality method for planting of dendrobium candidum |
| CN108450179A (en) * | 2018-03-30 | 2018-08-28 | 奥格生物技术（六安）有限公司 | A kind of preparation method of selenium-enriched officinal dendrobium stem flower |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010178644A (en) * | 2009-02-04 | 2010-08-19 | Ueno Engei:Kk | Method for supplying water to plant, and hydroponics system |
| CN103004409A (en) * | 2012-12-07 | 2013-04-03 | 安徽新津铁皮石斛开发有限公司 | Method for natural wild dendrobium officinale Kimura et Migo cultivation management |
| CN103283428A (en) * | 2012-02-29 | 2013-09-11 | 马雄英 | Greenhouse artificial cultivation method of dendrobium |
| CN103688830A (en) * | 2013-12-06 | 2014-04-02 | 海南松青生物科技有限公司 | Method for rapidly rooting and sprouting tissue culture seedlings of dendrobium officinale |
| CN103733958A (en) * | 2013-12-17 | 2014-04-23 | 广西森仙源生物科技有限公司 | Method for transplanting tissue culture seedlings of dendrobium officinale |
| CN103918536A (en) * | 2014-04-30 | 2014-07-16 | 成都润惠农业开发有限公司 | Planting method for Dendrobium officinale |
| CN103999672A (en) * | 2014-05-22 | 2014-08-27 | 普洱市民族传统医药研究所 | Pollution-free method for planting dendrobium nobile lindl |
| CN104025987A (en) * | 2014-06-24 | 2014-09-10 | 韶关车八岭农业科技有限公司 | Dendrobium officinale planting method |
| CN104322257A (en) * | 2014-10-29 | 2015-02-04 | 安徽牧龙山生态旅游开发股份有限公司 | Method for normatively planting dendrobium officinale in simple greenhouse |
-
2015
- 2015-02-11 CN CN201510070379.8A patent/CN104686308B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010178644A (en) * | 2009-02-04 | 2010-08-19 | Ueno Engei:Kk | Method for supplying water to plant, and hydroponics system |
| CN103283428A (en) * | 2012-02-29 | 2013-09-11 | 马雄英 | Greenhouse artificial cultivation method of dendrobium |
| CN103004409A (en) * | 2012-12-07 | 2013-04-03 | 安徽新津铁皮石斛开发有限公司 | Method for natural wild dendrobium officinale Kimura et Migo cultivation management |
| CN103688830A (en) * | 2013-12-06 | 2014-04-02 | 海南松青生物科技有限公司 | Method for rapidly rooting and sprouting tissue culture seedlings of dendrobium officinale |
| CN103733958A (en) * | 2013-12-17 | 2014-04-23 | 广西森仙源生物科技有限公司 | Method for transplanting tissue culture seedlings of dendrobium officinale |
| CN103918536A (en) * | 2014-04-30 | 2014-07-16 | 成都润惠农业开发有限公司 | Planting method for Dendrobium officinale |
| CN103999672A (en) * | 2014-05-22 | 2014-08-27 | 普洱市民族传统医药研究所 | Pollution-free method for planting dendrobium nobile lindl |
| CN104025987A (en) * | 2014-06-24 | 2014-09-10 | 韶关车八岭农业科技有限公司 | Dendrobium officinale planting method |
| CN104322257A (en) * | 2014-10-29 | 2015-02-04 | 安徽牧龙山生态旅游开发股份有限公司 | Method for normatively planting dendrobium officinale in simple greenhouse |
Non-Patent Citations (3)
| Title |
|---|
| 刘建春: "《抗衰老秘籍 认识衰老规律 延长人类生命》", 30 June 2013 * |
| 杨明志等: "《中国药用石斛标准研究与应用》", 31 December 2012 * |
| 林焰: "《园林花木景观应用图册 草本分册》", 31 August 2014 * |
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| CN106565323A (en) * | 2016-10-27 | 2017-04-19 | 安徽省霍山县祥佑堂中药材开发有限公司 | Special liquid fertilizer for dendrobe in field planting period |
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