CN104686308B - A kind of breeding method of dendrobium candidum - Google Patents
A kind of breeding method of dendrobium candidum Download PDFInfo
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- CN104686308B CN104686308B CN201510070379.8A CN201510070379A CN104686308B CN 104686308 B CN104686308 B CN 104686308B CN 201510070379 A CN201510070379 A CN 201510070379A CN 104686308 B CN104686308 B CN 104686308B
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- 241000026010 Dendrobium candidum Species 0.000 title claims abstract description 25
- 238000009395 breeding Methods 0.000 title claims abstract description 15
- 238000012258 culturing Methods 0.000 claims abstract description 24
- 239000011159 matrix material Substances 0.000 claims abstract description 12
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims abstract description 5
- 239000000375 suspending agent Substances 0.000 claims abstract description 5
- 238000009423 ventilation Methods 0.000 claims abstract description 5
- 239000005842 Thiophanate-methyl Substances 0.000 claims abstract description 4
- QGHREAKMXXNCOA-UHFFFAOYSA-N thiophanate-methyl Chemical compound COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC QGHREAKMXXNCOA-UHFFFAOYSA-N 0.000 claims abstract description 4
- 238000007654 immersion Methods 0.000 claims abstract description 3
- 235000010204 pine bark Nutrition 0.000 claims description 24
- 239000003337 fertilizer Substances 0.000 claims description 10
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 9
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 9
- 241001330002 Bambuseae Species 0.000 claims description 9
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 9
- 239000011425 bamboo Substances 0.000 claims description 9
- 239000003610 charcoal Substances 0.000 claims description 9
- 239000004575 stone Substances 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 210000003608 fece Anatomy 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 238000000855 fermentation Methods 0.000 claims description 5
- 230000004151 fermentation Effects 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 4
- 241000255789 Bombyx mori Species 0.000 claims description 4
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 4
- 241001494479 Pecora Species 0.000 claims description 4
- 229910021538 borax Inorganic materials 0.000 claims description 4
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims description 4
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 4
- 239000011790 ferrous sulphate Substances 0.000 claims description 4
- 230000012010 growth Effects 0.000 claims description 4
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 4
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 4
- 239000010871 livestock manure Substances 0.000 claims description 4
- 229960001471 sodium selenite Drugs 0.000 claims description 4
- 235000015921 sodium selenite Nutrition 0.000 claims description 4
- 239000011781 sodium selenite Substances 0.000 claims description 4
- 239000004328 sodium tetraborate Substances 0.000 claims description 4
- 235000010339 sodium tetraborate Nutrition 0.000 claims description 4
- 239000007921 spray Substances 0.000 claims description 4
- 229960001781 ferrous sulfate Drugs 0.000 claims description 3
- 230000002262 irrigation Effects 0.000 claims description 3
- 238000003973 irrigation Methods 0.000 claims description 3
- 229940071125 manganese acetate Drugs 0.000 claims description 3
- UOGMEBQRZBEZQT-UHFFFAOYSA-L manganese(2+);diacetate Chemical compound [Mn+2].CC([O-])=O.CC([O-])=O UOGMEBQRZBEZQT-UHFFFAOYSA-L 0.000 claims description 3
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 3
- 229960001763 zinc sulfate Drugs 0.000 claims description 3
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 claims 1
- VLAPMBHFAWRUQP-UHFFFAOYSA-L molybdic acid Chemical compound O[Mo](O)(=O)=O VLAPMBHFAWRUQP-UHFFFAOYSA-L 0.000 claims 1
- 238000004140 cleaning Methods 0.000 abstract description 3
- 210000001519 tissue Anatomy 0.000 description 18
- 230000000694 effects Effects 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 150000004676 glycans Chemical class 0.000 description 4
- 229920001282 polysaccharide Polymers 0.000 description 4
- 239000005017 polysaccharide Substances 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 3
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 description 3
- 235000018660 ammonium molybdate Nutrition 0.000 description 3
- 239000011609 ammonium molybdate Substances 0.000 description 3
- 229940010552 ammonium molybdate Drugs 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 210000002784 stomach Anatomy 0.000 description 3
- 241001523681 Dendrobium Species 0.000 description 2
- 241001076416 Dendrobium tosaense Species 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 241000233855 Orchidaceae Species 0.000 description 2
- 238000004061 bleaching Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 208000002177 Cataract Diseases 0.000 description 1
- 206010010144 Completed suicide Diseases 0.000 description 1
- 241000938605 Crocodylia Species 0.000 description 1
- 241000237858 Gastropoda Species 0.000 description 1
- 201000007100 Pharyngitis Diseases 0.000 description 1
- 241000256626 Pterygota <winged insects> Species 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 229910000746 Structural steel Inorganic materials 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 208000031971 Yin Deficiency Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- VQWQYXBWRCCZGX-UHFFFAOYSA-N acetic acid;manganese Chemical compound [Mn].CC(O)=O.CC(O)=O VQWQYXBWRCCZGX-UHFFFAOYSA-N 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 238000013475 authorization Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
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- 238000013461 design Methods 0.000 description 1
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- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 201000001881 impotence Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 229940060184 oil ingredients Drugs 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 206010036067 polydipsia Diseases 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 241000894007 species Species 0.000 description 1
- GOLXNESZZPUPJE-UHFFFAOYSA-N spiromesifen Chemical compound CC1=CC(C)=CC(C)=C1C(C(O1)=O)=C(OC(=O)CC(C)(C)C)C11CCCC1 GOLXNESZZPUPJE-UHFFFAOYSA-N 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- WQSRXNAKUYIVET-UHFFFAOYSA-N sulfuric acid;zinc Chemical compound [Zn].OS(O)(=O)=O WQSRXNAKUYIVET-UHFFFAOYSA-N 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- YFNCATAIYKQPOO-UHFFFAOYSA-N thiophanate Chemical compound CCOC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OCC YFNCATAIYKQPOO-UHFFFAOYSA-N 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a kind of breeding method of dendrobium candidum, including candidum tissue culturing seedling field planting step：Long 5 7cm healthy and strong candidum tissue culturing seedling is taken out from tissue culture bottle, after cleaning, the root of the candidum tissue culturing seedling is put into 1000 times of thiophanate-methyl suspending agents of liquid 50% to carry out disinfection, after immersion 10 minutes, dry in the air and put in ventilation of sheltering from heat or light, planted after the coring color of the candidum tissue culturing seedling bleaches completely in the matrix in seedbed.The breeding method for the dendrobium candidum that the present invention is provided is at least simple to operate, is conducive to commercial application.
Description
Technical field
The present invention relates to a kind of breeding method of plant, more particularly to a kind of breeding method of dendrobium candidum.
Background technology
Dendrobium candidum (Dendrobium officinale Kimura et Migo) is China's traditional Chinese medicine, is orchid family
(Orchidaceae) medicinal plant rare in Dendrobium (Dendrobium), is distributed mainly on Guizhou, Yunnan, Guangxi, river
The ground such as west, Anhui and Hunan.According to《Compendium of Materia Medica》Record, dendrobium candidum have " cure mainly in wound, tonifying five zang organs consumptive disease, strong cloudy strengthening the essence,
In mending never sufficient, flat stomach gas, long muscle intelligence development remove shy, macrobiosis of making light of one's life by commiting suicide the effect of effect ".According to version in 2010《The Chinese people are total to
With state's pharmacopeia》Record, dendrobium candidum has the effects such as reinforcing stomach reg fluid and nourishing Yin and clearing heat, is usually used in pyreticosis Tianjin wound, dry polydipsia, stomach
Asthenic yin, deficiency of food retch, after being ill abnormal heat do not move back, fire excess from yin deficiency, hectic fever due to yin labor heat, mesh secretly fail to understand and the disease such as muscles and bones impotence treatment.
Its stem is used as medicine, and to treatment pharyngolaryngitis, improves body immunity or even suppression tumour all with remarkable result, has won fame both at home and abroad
Health-care good product.
Dendrobium candidum is strict to growing environment requirement, the warm cool high humidity area of 250-900 meters of height above sea level is distributed in mostly, by small
The influence of the climatic factors such as relative humidity, temperature, illumination in environment is larger, due to needing to have little understanding to its growing environment, people
Work cultivation is more difficult, wild resource is relied primarily on for a long time, current dendrobium officinale resource is endangered.Dendrobium candidum
It is《Wild plant international trade pact in imminent danger》Protection species and China second class protection endangered plants, be to forbid trade in the world
Easy.The regularity of distribution including polysaccharide in Different Organs main to the research in terms of dendrobium candidum water-soluble polysaccharide at present, carry
Take, separate, purifying and key component Structural Identification, and in terms of pharmacological effect, result of study shows different planting
The polyoses content of lower dendrobium candidum can be variant.
Modern medicine study proves that the polysaccharide in dendrobium candidum can significantly improve human body SOD levels, reduces LPO levels, energy
Promote T cell growth, so as to play a part of that enhancing is immune and anti-aging, have to tonic after old man, cancer patient chemicotherapy
Significant effect.There is good, definite curative effect in terms for the treatment of diabetes, cataract, chronic pharyngitis.Authorization Notice No.
Patent for CN102172152B using illumination, temperature, fertilizer while sprayed, water is poured, sound stimulation, and just sprouted with reference to bud
Send out time control flower bud and the method to flowering parts manual removal bud carries out cultivation management, as a result show that this method can be improved
The content of polysaccharide in dendrobium candidum, but this method complex operation, are unfavorable for industrialization production application.
Accordingly, it is desirable to provide it is a kind of simple to operate, beneficial to the breeding method of the dendrobium candidum of commercial application.
The content of the invention
Therefore, the present invention proposes a kind of cultivation at least one of new dendrobium candidum that can be solved the above problems
Method.
According to an aspect of the invention, there is provided a kind of breeding method of dendrobium candidum, including
Candidum tissue culturing seedling is colonized step：Long 5-7cm healthy and strong candidum tissue culturing seedling is taken out from tissue culture bottle, washed
After net, the root of the candidum tissue culturing seedling is put into 1000 times of thiophanate-methyl suspending agents of liquid 50% and carried out disinfection, immersion 10
After minute, dry in the air and put in ventilation of sheltering from heat or light, the base into seedbed is planted after the coring color of the candidum tissue culturing seedling bleaches completely
In matter.
Alternatively, according to an embodiment of the invention, further wrapped after the candidum tissue culturing seedling field planting step
Include the management process after field planting：
Kept for a week do not spray water, until the root end of the candidum tissue culturing seedling starts just to start spray after growth
Fill, keep the humid control of greenhouse in 60%-80%, its temperature control on daytime at 22-30 DEG C, night according to natural temperature, and
Winter nights minimum temperature is maintained at subzero more than 3 DEG C.
Alternatively, according to an embodiment of the invention, the matrix includes stone, pine bark, pine bark and bamboo charcoal,
Wherein, the volume ratio of each component is stone：Pine bark:Pine bark:Bamboo charcoal=1:6:1:2.
Alternatively, according to an embodiment of the invention, it is 13-15 centimetres that the bottom of the matrix, which is provided with thickness,
Pine bark, the surface of the matrix is provided with the pine bark that thickness is 1-2cm.
Alternatively, according to an embodiment of the invention, step of applying fertilizer is further comprised：Annual March, August part are each
Apply a fertilizer to the candidum tissue culturing seedling.
Alternatively, according to an embodiment of the invention, the fertilizer is by borax, sodium selenite, ammonium molybdate, acetic acid
Manganese, ferrous sulfate, zinc sulfate are well mixed with oil cake, sheep manure, rabbit excrement, silkworm faeces, mix suitable quantity of water, and inoculation biological bacteria loads fermentation
What pond fermentation was obtained for 3-4 months.
The breeding method for the dendrobium candidum that the present invention is provided is at least simple to operate, is conducive to commercial application.
Embodiment
The invention provides many applicable creative concepts, the creative concept can be reflected in a large number of on specific
Hereinafter.Specific embodiment described in following embodiments of the present invention is only as embodiment of the invention
Exemplary illustration, and be not meant to limit the scope of the invention.
With reference to specific embodiment, the invention will be further described.
A kind of breeding method of dendrobium candidum, including candidum tissue culturing seedling field planting step：By long 5-7cm healthy and strong iron sheet
Dendrobe tissue culture seedling is taken out from tissue culture bottle, and after cleaning, the root of the candidum tissue culturing seedling is put into 1000 times of methyl of liquid 50%
Thiophanate suspending agent carries out disinfection, and after soaking 10 minutes, dries in the air and puts in ventilation of sheltering from heat or light, treat the coring of the candidum tissue culturing seedling
Color is planted after bleaching completely in the matrix in seedbed.The matrix includes stone, pine bark, pine bark and bamboo charcoal, wherein, each group
The volume ratio divided is stone：Pine bark:Pine bark:Bamboo charcoal=1:6:1:2.The bottom of the matrix is provided with thickness for 13-
15 centimetres of pine bark, the surface of the matrix is provided with the pine bark that thickness is 1-2cm.
Further comprise the management process after field planting after the candidum tissue culturing seedling field planting step：Kept for a week do not spray
Water, until the root end of the candidum tissue culturing seedling starts just to start sprinkling irrigation after growth, keeps the humid control of greenhouse to exist
60%-80%, its temperature control on daytime is at 22-30 DEG C, and night is according to natural temperature, and winter nights minimum temperature is maintained at zero
Lower more than 3 DEG C.
The breeding method of the dendrobium candidum further comprises step of applying fertilizer：Annual March, August part are each to the iron sheet
Dendrobe tissue culture seedling applies a fertilizer.The fertilizer is by borax, sodium selenite, ammonium molybdate, manganese acetate, ferrous sulfate, sulfuric acid
Zinc is well mixed with oil cake, sheep manure, rabbit excrement, silkworm faeces, mixes suitable quantity of water, and inoculation biological bacteria loads fermenting tank for fermentation and obtained for 3-4 months
Arrive.
The breeding method of the dendrobium candidum provided further below the present invention is explained in detail：
Preparing nursery bed：Build greenhouse, ceiling and surrounding are with 12 Anti-fogging Films, and ceiling covering shade density 80% or so is sheltered from heat or light
Net.
Seedbed is provided with canopy
1. the specification in seedbed is that 60cm is high, wide 1.5m, long 28m.
2. seedbed frame is angle iron frame, plastic wire bound edge.
3. matrix：Bottom is plugged with the pine bark of 3-5cm specifications, high-order 14cm, and upper strata is 0.5-0.8cm pine bark, thick
Spend for 1cm or so.
The raw material of hypothallus is made and organic fertilizer is used
Matrix formulations：2-3cm stone, 3-5cm pine bark, 0.5-0.8cm pine bark, 3-5cm bamboo charcoal, matrix
Volume ratio is 1:6:1:2, enter after 120 DEG C of high-temperature sterilizations and use.
Borax, sodium selenite, ammonium molybdate, manganese acetate, ferrous sulfate, zinc sulfate and oil cake, sheep manure, rabbit excrement, silkworm faeces are mixed
Close uniform, mix suitable quantity of water, inoculation biological bacteria loads fermenting tank for fermentation 3-4 months, 20 grams every clump, and annual March, August part are each
Apply once.
Field planting：By taking-up of the long 5-7cm healthy and strong candidum tissue culturing seedling from tissue culture bottle gently, after cleaning, root is put
Enter 1000 times of thiophanate-methyl suspending agents of liquid 50% to carry out disinfection, after soaking 10 minutes, dry in the air to put in ventilation of sheltering from heat or light and treat coring face
Color is planted in seedbed after bleaching completely.
Management after field planting：Seed keeps not spraying water in a week, starts just to start sprinkling irrigation after growth etc. root end, keeps
The humid control of greenhouse is in 60%-80%, and its temperature control on daytime is at 22-30 DEG C, and night is according to natural temperature, and winter nights
Minimum temperature is maintained at subzero more than 3 DEG C.
The fertilising process of seed
4th, insect pest preventing and controlling
(1) the maximum insect pest of dendrobium candidum is the reptiles such as snail, using plastic ware is placed on every pin in seedbed, in device
Salt is added in ware, prevents insect pest from entering in seedbed.
(2) preventing and treating of winged insect, using day sun can trapping lamp, greenhouse periphery have specification be mesh 0.03cm fly net every
From.
It is worth noting that, the present invention and its each product, raw material etc. be what prior art was provided.Its composition such as without
Special instruction, those of ordinary skill in the art can obtain according to conventional technical means.
It should be noted that the present invention will be described rather than limits the invention for above-described embodiment, and this
Art personnel can design alternative embodiment without departing from the scope of the appended claims.In claim
In, any reference symbol between bracket should not be configured to limitations on claims.Word "comprising" is not excluded for depositing
In element or step not listed in the claims.Word "a" or "an" before element do not exclude the presence of it is multiple this
The element of sample.The use of word first, second, and third does not indicate that any order.These words can be construed to title.
Claims (3)
1. a kind of breeding method of dendrobium candidum, including：
Candidum tissue culturing seedling is colonized step：Long 5-7cm healthy and strong candidum tissue culturing seedling is taken out from tissue culture bottle, cleaned
Afterwards, the root of the candidum tissue culturing seedling is put into 1000 times of thiophanate-methyl suspending agents of liquid 50% to carry out disinfection, immersion 10
After minute, dry in the air and put in ventilation of sheltering from heat or light, the base into seedbed is planted after the coring color of the candidum tissue culturing seedling bleaches completely
In matter；
Further comprise the management process after field planting after the candidum tissue culturing seedling field planting step：
Kept for a week do not spray water, until the root end of the candidum tissue culturing seedling starts just to start sprinkling irrigation after growth, protected
The humid control of greenhouse is held in 60%-80%, its temperature control on daytime is at 22-30 DEG C, and night is according to natural temperature, and night in winter
Late minimum temperature is maintained at subzero more than 3 DEG C；
The matrix includes stone, the first pine bark, the second pine bark and bamboo charcoal, wherein, the volume ratio of each component is stone：
First pine bark:Second pine bark:Bamboo charcoal=1:6:1:2；
The stone is the stone of 2-3cm specifications, and first pine bark is the pine bark of 3-5cm specifications, second pine
Bark is the pine bark of 0.5-0.8cm specifications, and the bamboo charcoal is the bamboo charcoal of 3-5cm specifications.
2. the breeding method of the dendrobium candidum according to claim 1, further comprises step of applying fertilizer：Annual March,
August is each to a candidum tissue culturing seedling fertilizer of application.
3. the breeding method of the dendrobium candidum according to claim 2, the fertilizer is by borax, sodium selenite, molybdic acid
Ammonium, manganese acetate, ferrous sulfate, zinc sulfate are well mixed with oil cake, sheep manure, rabbit excrement, silkworm faeces, mix suitable quantity of water, are inoculated with biological bacteria
Load what fermenting tank for fermentation was obtained for 3-4 months.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105248262B (en) * | 2015-10-08 | 2018-01-09 | 浙江省农业科学院 | A kind of pine bark charcoal matrix for officinal dendrobium stem plantation |
| CN106035028A (en) * | 2016-06-30 | 2016-10-26 | 上海孙桥农业科技股份有限公司 | Cultivation substrate for dendrobium officinale and preparation method |
| CN106565323A (en) * | 2016-10-27 | 2017-04-19 | 安徽省霍山县祥佑堂中药材开发有限公司 | Special liquid fertilizer for dendrobe in field planting period |
| CN106942031A (en) * | 2016-12-30 | 2017-07-14 | 浙江金石生物科技有限公司 | A kind of high-quality method for planting of dendrobium candidum |
| CN108450179A (en) * | 2018-03-30 | 2018-08-28 | 奥格生物技术（六安）有限公司 | A kind of preparation method of selenium-enriched officinal dendrobium stem flower |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP5246576B2 (en) * | 2009-02-04 | 2013-07-24 | 有限会社上野園芸 | Water supply method for plants and hydroponics system |
| CN103283428A (en) * | 2012-02-29 | 2013-09-11 | 马雄英 | Greenhouse artificial cultivation method of dendrobium |
| CN103004409A (en) * | 2012-12-07 | 2013-04-03 | 安徽新津铁皮石斛开发有限公司 | Method for natural wild dendrobium officinale Kimura et Migo cultivation management |
| CN103688830B (en) * | 2013-12-06 | 2015-11-25 | 海南松青生物科技有限公司 | Method for rapidly rooting and sprouting tissue culture seedlings of dendrobium officinale |
| CN103733958A (en) * | 2013-12-17 | 2014-04-23 | 广西森仙源生物科技有限公司 | Method for transplanting tissue culture seedlings of dendrobium officinale |
| CN103918536B (en) * | 2014-04-30 | 2016-01-13 | 成都润惠农业开发有限公司 | The implantation methods of dendrobium candidum |
| CN103999672B (en) * | 2014-05-22 | 2016-03-23 | 普洱市民族传统医药研究所 | A kind of method of non-polluted planting dendrobium candidum |
| CN104025987A (en) * | 2014-06-24 | 2014-09-10 | 韶关车八岭农业科技有限公司 | Dendrobium officinale planting method |
| CN104322257B (en) * | 2014-10-29 | 2016-08-17 | 安徽牧龙山生态旅游开发股份有限公司 | A kind of method of dendrobium candidum simple plastic greenhouse GAP |
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