CN104025987A - Dendrobium officinale planting method - Google Patents

Dendrobium officinale planting method Download PDF

Info

Publication number
CN104025987A
CN104025987A CN201410284005.1A CN201410284005A CN104025987A CN 104025987 A CN104025987 A CN 104025987A CN 201410284005 A CN201410284005 A CN 201410284005A CN 104025987 A CN104025987 A CN 104025987A
Authority
CN
China
Prior art keywords
seedling
plant
matrix
time
days
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201410284005.1A
Other languages
Chinese (zh)
Inventor
魏炳潮
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shaoguan Che Baling Agricultural Science And Technology Co Ltd
Original Assignee
Shaoguan Che Baling Agricultural Science And Technology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shaoguan Che Baling Agricultural Science And Technology Co Ltd filed Critical Shaoguan Che Baling Agricultural Science And Technology Co Ltd
Priority to CN201410284005.1A priority Critical patent/CN104025987A/en
Publication of CN104025987A publication Critical patent/CN104025987A/en
Pending legal-status Critical Current

Links

Classifications

    • Y02P60/216

Abstract

The invention relates to a dendrobium officinale planting method which includes: building a planting seedbed, selecting and processing substrate, preparing seedlings, performing artificial pollination, harvesting capsules, performing capsule processing and inoculation, performing protocorm value-added culture, performing strong seedling rooting culture, taking strong seedlings out of bottles and planting, performing seedling management on the seedlings, managing the grown-up seedlings again, harvesting, checking and storing, cutting fresh dendrobium officinale into sections after impurity removing and cleaning, drying at low temperature of 60 DEG C to allow the water content of the dendrobium officinale not to exceed 12%, and placing the dried dendrobium officinale at a ventilated and dry place to prevent dampness. The dendrobium officinale planting method is high in economical benefit, low in planting cost and easy to popularize.

Description

Dendrobium candidum implantation methods
Technical field
The invention belongs to agricultural planting technique field, relate to a kind of dendrobium candidum implantation methods.
Background technology
Dendrobium candidum is one of kind the most precious in China's the orchid family Dendrobium Sw.Because having, it strengthens immunity of organisms, antitumor, anti-ageing and effectively alleviate the effects such as hepatic injury; in 2010, in version < < Pharmacopoeia of People's Republic of China > >, classified separately as medicinal material simply; due to the destruction of artificially excessively excavating with ecotope; in addition himself reproduction rate is low; wild dendrobium candidum resource is day by day exhausted, by country, is classified as the rare or endangered species that secondary is laid special stress on protecting.Now, artificial planting dendrobium candidum has become the main path that meets great market demand.
Summary of the invention
In order to overcome the above-mentioned shortcoming of prior art, the invention provides a kind of dendrobium candidum implantation methods, it can improve the survival rate of dendrobium candidum greatly, has saved the time of cultivating, has improved economic benefit and be beneficial to implant mass.
The technical solution adopted for the present invention to solve the technical problems is: a kind of dendrobium candidum implantation methods, and its processing step is:
The first step: build plantation seedbed, adopt angle bar or the wooden bedstead of doing of bamboo, fixing bedside 1m, long 5m; The height of bed can be high definite according to the ridge of canopy, generally should be lower than 20cm not higher than 70cm, and bed surface plastics plain net is as the supporting surface of matrix, and top, seedbed 50 to the 80cm high shower nozzles that spraying is housed, can control spray time;
Second step: the selection of matrix and processing, select pine bark as cultivation matrix, matrix will be sterilized before using, and matrix can be used the method disinfections such as boiling, Exposure to Sunlight and immersion; Water content of substrate is 60% left and right, and matrix is layered on bed surface, shakeouts, and thickness is generally 5 to 8cm;
The 3rd step: seedling is prepared, and the dendrobium candidum seedling of the autonomous seed selection in variety adoption base is cultivated Miao Yuan as group;
The 4th step: artificial pollination, select more than 2 years without damage by disease and insect, and possesses the fine individual plant of this kind characteristic feature, early flowering season is chosen white anther cap with toothpick, to expose yellow pollen, with toothpick, dip, invest gently the recess of maternal plant gynoecium, the relevant informations such as the rear listing mark father of pollination, female parent and pollination time;
The 5th step: capsule is gathered, pollination is by 40 to 60 days, when outstanding achievement appearance is started to become when light yellow from green, fruit maturation and cracking is not gathered in time; After gathering, with clean paper handkerchief, wrap, be placed in 5~10 ℃ of refrigerators stand-by, the holding time is unsuitable long;
The 6th step: capsule is processed and inoculation, the capsule of gathering is placed in 75% alcohol and carries out surface sterilization 30 seconds, with sterile water wash once after, be placed in 0.1% mercuric chloride and sterilize 20 minutes, in disinfecting process, want intermittent oscillation, that sterilization is abundant, after finishing, use aseptic water washing 3~5 times, under aseptic condition, fruit is cut, seed is spread on the MS medium that 5-10 bottle configured equably, and culture environment is: 24~26 ℃ of temperature, relative moisture 60%, illumination is 1000 luxs, and the light application time of every day is 12 hours.Through about 50 days, in bottle, grow protocorm, observed and recorded differential growth situation;
The 7th step: protocorm increment is cultivated, the stalwartness will induction producing, the normal protocorm of growth color carries out cutting to be transferred to breed on MS minimal medium and expands bottle, and medium also needs to add 12% murphy juice and 0.5% methyl α-naphthyl acetate.Putting environment is 24~26 ℃ of temperature, and humidity is 60%, and illumination is 2000 luxs, and every day, light application time was 14 hours, cultivate 50~60 days, and in incubation, observed and recorded upgrowth situation;
The 8th step: strengthening seedling and rooting is cultivated, when the seedling 2~3cm of protocorm differentiation, by the size fractionation of seedling, transplant in MS medium (medium is containing 1% methyl α-naphthyl acetate) and cultivate, transplant 60 strains for every bottle, storing environment is: 24~26 ℃ of temperature, humidity is 60%, illumination is 2500~3000 luxs, illumination every day 18 hours, carries out culture of rootage with this understanding, within 60 days, observes afterwards and records height of seedling, the growing states such as root system number, when seedling reaches 4 more than leaf, more than plant height 3cm, during the above condition of 3 of roots, without carrying out hardening, can carry out outdoor planting;
The 9th step: bottle outlet, medium is taken out gently together with seedling, be placed in large basin and clean up with clear water, suitably classification is placed in 1000 times of liquid of 70% potassium permanganate and soaks seedling 5~10 minutes, is placed in to dry under scattered light to wait to plant to the root deliquescing of turning white for 5~7 hours;
The tenth step: plantation, the weather in Guangdong Province of take is standard, and in annual March~all can transplant by the end of November, best plantation period is spring, Qiu Liangji, and clump is planted, and take 3 to 4 strains as one clump, by 8cm * 10cm line-spacing, plants.During plantation, will handle with care, not break fleshy root as far as possible, planting depth is 2~3cm.Different brackets seedling is plantation separately, convenient management.According to different seedbeds pattern, it is 2.4 ten thousand clumps of left and right that every mu of booth is planted seedling quantity;
The 11 step: seedling management, transplant 7~15 days seedling initial stages, atomized water spray in canopy, keeps the interior humidity of canopy in 80% left and right, available 80 object sunshade net sunshades; Transplant 15~30 days, seedling grows new root, starts to send out sprouting, and the drenched matrix of watering is advisable, and in canopy, relative air humidity is advisable with 70~80%, available 70 order sunshade net sunshades, and note ventilating, now can execute slow-release fertilizer or organic fertilizer that N:P:K is 1:1:1; After 11~30 days, seedling starts to enter fast growing period, can spray composite fertilizer or the organic fertilizer of 1 1:1500 times of liquid in conjunction with watering at every turn, and cup is planted seedling and within general 1~2 day, drenched a water; Bed is planted seedling can drench a water in 2~5 days according to the moistening degree of matrix, can drench matrix top layer 2cm left and right for degree;
The 12 step: become seedling management in age,
During spring in 3~May, outside air temperature gos up gradually, air is more moistening, plant starts growth, basal part of stem is sprouted sprouting, grows new root, and now seedling increases gradually to liquid manure demand, a water of every mornings 7 point~10 spray during fine day, wetting stromal surface, and can be in conjunction with trickle, each pouring executed liquid manure one time, dilute 1000 times of liquid, within 10~15 days, spray a sterilization (worm) agent, full exposure, suitable shading when noon, sunlight was fierce, in canopy, keep ventilated, " going back to Nan Tian ", will carry out mandatory ventilation;
During summer in 6~August, weather is hot and humid, it is dendrobium candidum vigorous growth season, also be cultivation management important period, temperature is many 30 ℃ of left and right, this period is sunny, should avoid sunlight directly to shine, easily there is " sunscald " in carelessness slightly, every day, 11:00-16:00 canopy planted agent started two net shadings under normal circumstances, shading 30-50%, and improve ventilation condition in canopy, in hot weather plant body, water consumption is larger, should supplement in time, every day is except morning normal trickle, afternoon, post sunset increased pouring once, from 5-9 month, every two weeks is executed disposable fertilizer, depending on Changes in weather situation, determine liquid manure consumption, temperature is 35 ℃ of left and right, want discontinuity to sprinkle water earthward, with high humidity, lower the temperature.Note leaf spot and soft rot, discovery diseased plant is removed in time, avoids in high temperature and the fertilising of high light time;
During autumn in 9~November, temperature declines gradually, day and night temperature is about 5~10 ℃, temperature now and temperature range are extremely conducive to effective accumulation of organic principle in plant body, top grows termination leaf during this time, plant is from nourishing and growing toward reproductive growth transition, pseudobulb increases the thick increase stage, now should increase intensity of illumination in canopy, shorten the shading time, sufficient illumination is conducive to generation and the accumulation of nutriment, still likely there is high temperature in weather now, note also according to weather condition adjustment and ventilate, cooling, guaranteeing that matrix is not under the prerequisite of ponding, keep moistening, avoid the long-term overdrying of matrix, otherwise be also unfavorable for plant strain growth, regularly carry out artificial weeding and loose matrix, the insect pest of finding is carried out to artificial capture method,
During winter approached, progress into hypothermic phase 12~February, and now plant strain growth is slowly to stopping growing, and in stem stalk body, content of organics reaches maximum.Flower bud differentiation completes, and part stipes slowly completes bud elongation, inflorescence and flower bud development until bloom, and plant grows and slows down in the time of 10~15 ℃, in the time of≤10 ℃, stops growing, and can resist the microthermal climate of short-term≤5 ℃.Now full exposure in canopy, reduces trickle number of times in the time of≤10 ℃, within 7~10 days, is once advisable, and tries not to pour blade during operation.Within 10~15 days, drench once balanced fertile (2000 times of water liquid);
The 13 step: gather, suitable collecting time is June November to next year, adopts the old new and herb 2 kinds of modes of gathering of staying, carry out adopt old stay new, 20 months acrial part plant in vegetative period above of gathering;
The 14 step: check and accept and storage, after gathering, reject in time diseased plant, according to related standards such as < < Pharmacopoeia of People's Republic of China > >, company standard and purchase orders, detect the projects such as polysaccharide, moisture, agricultural chemicals, heavy-metal residual; Iron-sheet dendrobe fresh product is placed in shady and cool moist place;
The 15 step: fresh goods by removal of impurities, clean after low temperature drying at segment, 60 ℃, water content is no more than 12%, dry product is placed in aeration-drying place, protection against the tide.
In described the 8th step, according to seedling size, be divided into qualified clusters training seedling and high-quality seedling, described qualified clusters training seedling standard: 2 to 5 of roots, 4 leaves, plant height >=2.5cm, stem is thick >=0.2cm; Described high-quality seedling standard: 3 to 5 of roots, 6 leaves, plant height >=4.0cm, stem is thick >=0.3cm.
When described the tenth step, need to use inorganic fertilizer or organic fertilizer to apply fertilizer.
In described the tenth step, should be noted that in canopy and have higher humidity and weak illumination, matrix is not dry does not water, so that inducing rooting, field planting can be sprayed low concentration tpn after 3 days carries out prevention of damage by disease.
The invention has the beneficial effects as follows: the seedling active constituent content of selecting is high and stable, disease resistance is stablized by force, the conduct group of high yield suitable local plantation is cultivated Miao Yuan, guaranteed the survival rate of planting process seedling, there is higher economic benefit, and be conducive to establishing in large scale, have that economic benefit is high, planting cost is low, popularize easy feature.
Embodiment
Below in conjunction with embodiment, the present invention is further described.
A dendrobium candidum implantation methods, its processing step is:
The first step: build plantation seedbed, adopt angle bar or the wooden bedstead of doing of bamboo, fixing bedside 1m, long 5m; The height of bed can be high definite according to the ridge of canopy, generally should be lower than 20cm not higher than 70cm, and bed surface plastics plain net is as the supporting surface of matrix, and top, seedbed 50 to the 80cm high shower nozzles that spraying is housed, can control spray time;
Second step: the selection of matrix and processing, select pine bark as cultivation matrix, matrix will be sterilized before using, and matrix can be used the method disinfections such as boiling, Exposure to Sunlight and immersion; Water content of substrate is 60% left and right, and matrix is layered on bed surface, shakeouts, and thickness is generally 5 to 8cm;
The 3rd step: seedling is prepared, and the dendrobium candidum seedling of the autonomous seed selection in variety adoption base is cultivated Miao Yuan as group;
The 4th step: artificial pollination, select more than 2 years without damage by disease and insect, and possesses the fine individual plant of this kind characteristic feature, early flowering season is chosen white anther cap with toothpick, to expose yellow pollen, with toothpick, dip, invest gently the recess of maternal plant gynoecium, the relevant informations such as the rear listing mark father of pollination, female parent and pollination time;
The 5th step: capsule is gathered, pollination is by 40 to 60 days, when outstanding achievement appearance is started to become when light yellow from green, fruit maturation and cracking is not gathered in time; After gathering, with clean paper handkerchief, wrap, be placed in 5~10 ℃ of refrigerators stand-by, the holding time is unsuitable long;
The 6th step: capsule is processed and inoculation, the capsule of gathering is placed in 75% alcohol and carries out surface sterilization 30 seconds, with sterile water wash once after, be placed in 0.1% mercuric chloride and sterilize 20 minutes, in disinfecting process, want intermittent oscillation, that sterilization is abundant, after finishing, use aseptic water washing 3~5 times, under aseptic condition, fruit is cut, seed is spread on the MS medium that 5-10 bottle configured equably, and culture environment is: 24~26 ℃ of temperature, relative moisture 60%, illumination is 1000 luxs, and the light application time of every day is 12 hours.Through about 50 days, in bottle, grow protocorm, observed and recorded differential growth situation;
The 7th step: protocorm increment is cultivated, the stalwartness will induction producing, the normal protocorm of growth color carries out cutting to be transferred to breed on MS minimal medium and expands bottle, and medium also needs to add 12% murphy juice and 0.5% methyl α-naphthyl acetate.Putting environment is 24~26 ℃ of temperature, and humidity is 60%, and illumination is 2000 luxs, and every day, light application time was 14 hours, cultivate 50~60 days, and in incubation, observed and recorded upgrowth situation;
The 8th step: strengthening seedling and rooting is cultivated, when the seedling 2~3cm of protocorm differentiation, by the size fractionation of seedling, transplant in MS medium (medium is containing 1% methyl α-naphthyl acetate) and cultivate, transplant 60 strains for every bottle, storing environment is: 24~26 ℃ of temperature, humidity is 60%, illumination is 2500~3000 luxs, illumination every day 18 hours, carries out culture of rootage with this understanding, within 60 days, observes afterwards and records height of seedling, the growing states such as root system number, when seedling reaches 4 more than leaf, more than plant height 3cm, during the above condition of 3 of roots, without carrying out hardening, can carry out outdoor planting;
The 9th step: bottle outlet, medium is taken out gently together with seedling, be placed in large basin and clean up with clear water, suitably classification is placed in 1000 times of liquid of 70% potassium permanganate and soaks seedling 5~10 minutes, is placed in to dry under scattered light to wait to plant to the root deliquescing of turning white for 5~7 hours;
The tenth step: plantation, the weather in Guangdong Province of take is standard, and in annual March~all can transplant by the end of November, best plantation period is spring, Qiu Liangji, and clump is planted, and take 3 to 4 strains as one clump, by 8cm * 10cm line-spacing, plants.During plantation, will handle with care, not break fleshy root as far as possible, planting depth is 2~3cm.Different brackets seedling is plantation separately, convenient management.According to different seedbeds pattern, it is 2.4 ten thousand clumps of left and right that every mu of booth is planted seedling quantity;
The 11 step: seedling management, transplant 7~15 days seedling initial stages, atomized water spray in canopy, keeps the interior humidity of canopy in 80% left and right, available 80 object sunshade net sunshades; Transplant 15~30 days, seedling grows new root, starts to send out sprouting, and the drenched matrix of watering is advisable, and in canopy, relative air humidity is advisable with 70~80%, available 70 order sunshade net sunshades, and note ventilating, now can execute slow-release fertilizer or organic fertilizer that N:P:K is 1:1:1; After 11~30 days, seedling starts to enter fast growing period, can spray composite fertilizer or the organic fertilizer of 1 1:1500 times of liquid in conjunction with watering at every turn, and cup is planted seedling and within general 1~2 day, drenched a water; Bed is planted seedling can drench a water in 2~5 days according to the moistening degree of matrix, can drench matrix top layer 2cm left and right for degree;
The 12 step: become seedling management in age
During spring in 3~May, outside air temperature gos up gradually, air is more moistening, plant starts growth, basal part of stem is sprouted sprouting, grows new root, and now seedling increases gradually to liquid manure demand, a water of every mornings 7 point~10 spray during fine day, wetting stromal surface, and can be in conjunction with trickle, each pouring executed liquid manure one time, dilute 1000 times of liquid, within 10~15 days, spray a sterilization (worm) agent, full exposure, suitable shading when noon, sunlight was fierce, in canopy, keep ventilated, " going back to Nan Tian ", will carry out mandatory ventilation;
During summer in 6~August, weather is hot and humid, it is dendrobium candidum vigorous growth season, also be cultivation management important period, temperature is many 30 ℃ of left and right, this period is sunny, should avoid sunlight directly to shine, easily there is " sunscald " in carelessness slightly, every day, 11:00-16:00 canopy planted agent started two net shadings under normal circumstances, shading 30-50%, and improve ventilation condition in canopy, in hot weather plant body, water consumption is larger, should supplement in time, every day is except morning normal trickle, afternoon, post sunset increased pouring once, from 5-9 month, every two weeks is executed disposable fertilizer, depending on Changes in weather situation, determine liquid manure consumption, temperature is 35 ℃ of left and right, want discontinuity to sprinkle water earthward, with high humidity, lower the temperature.Note leaf spot and soft rot, discovery diseased plant is removed in time, avoids in high temperature and the fertilising of high light time;
During autumn in 9~November, temperature declines gradually, day and night temperature is about 5~10 ℃, temperature now and temperature range are extremely conducive to effective accumulation of organic principle in plant body, top grows termination leaf during this time, plant is from nourishing and growing toward reproductive growth transition, pseudobulb increases the thick increase stage, now should increase intensity of illumination in canopy, shorten the shading time, sufficient illumination is conducive to generation and the accumulation of nutriment, still likely there is high temperature in weather now, note also according to weather condition adjustment and ventilate, cooling, guaranteeing that matrix is not under the prerequisite of ponding, keep moistening, avoid the long-term overdrying of matrix, otherwise be also unfavorable for plant strain growth, regularly carry out artificial weeding and loose matrix, the insect pest of finding is carried out to artificial capture method,
During winter approached, progress into hypothermic phase 12~February, and now plant strain growth is slowly to stopping growing, and in stem stalk body, content of organics reaches maximum.Flower bud differentiation completes, and part stipes slowly completes bud elongation, inflorescence and flower bud development until bloom, and plant grows and slows down in the time of 10~15 ℃, in the time of≤10 ℃, stops growing, and can resist the microthermal climate of short-term≤5 ℃.Now full exposure in canopy, reduces trickle number of times in the time of≤10 ℃, within 7~10 days, is once advisable, and tries not to pour blade during operation.Within 10~15 days, drench once balanced fertile (2000 times of water liquid);
The 13 step: gather, suitable collecting time is June November to next year, adopts the old new and herb 2 kinds of modes of gathering of staying, carry out adopt old stay new, 20 months acrial part plant in vegetative period above of gathering;
The 14 step: check and accept and storage, after gathering, reject in time diseased plant, according to related standards such as < < Pharmacopoeia of People's Republic of China > >, company standard and purchase orders, detect the projects such as polysaccharide, moisture, agricultural chemicals, heavy-metal residual; Iron-sheet dendrobe fresh product is placed in shady and cool moist place;
The 15 step: fresh goods by removal of impurities, clean after low temperature drying at segment, 60 ℃, water content is no more than 12%, dry product is placed in aeration-drying place, protection against the tide.
In described the 8th step, according to seedling size, be divided into qualified clusters training seedling and high-quality seedling, described qualified clusters training seedling standard: 2 to 5 of roots, 4 leaves, plant height >=2.5cm, stem is thick >=0.2cm; Described high-quality seedling standard: 3 to 5 of roots, 6 leaves, plant height >=4.0cm, stem is thick >=0.3cm.
When described the tenth step, need to use inorganic fertilizer or organic fertilizer to apply fertilizer.
In described the tenth step, should be noted that in canopy and have higher humidity and weak illumination, matrix is not dry does not water, so that inducing rooting, field planting can be sprayed low concentration tpn after 3 days carries out prevention of damage by disease.
The seedling active constituent content that the present invention selects is high and stable, disease resistance is stablized by force, the conduct group of high yield suitable local plantation is cultivated Miao Yuan, guaranteed the survival rate of planting process seedling, there is higher economic benefit, and be conducive to establishing in large scale, have that economic benefit is high, planting cost is low, popularize easy feature.

Claims (4)

1. a dendrobium candidum implantation methods, is characterized in that processing step is:
The first step: build plantation seedbed, adopt angle bar or the wooden bedstead of doing of bamboo, fixing bedside 1m, long 5m; The height of bed can be high definite according to the ridge of canopy, generally should be lower than 20cm not higher than 70cm, and bed surface plastics plain net is as the supporting surface of matrix, and top, seedbed 50 to the 80cm high shower nozzles that spraying is housed, can control spray time;
Second step: the selection of matrix and processing, select pine bark as cultivation matrix, matrix will be sterilized before using, and matrix can be used the method disinfections such as boiling, Exposure to Sunlight and immersion; Water content of substrate is 60% left and right, and matrix is layered on bed surface, shakeouts, and thickness is generally 5 to 8cm;
The 3rd step: seedling is prepared, and the dendrobium candidum seedling of the autonomous seed selection in variety adoption base is cultivated Miao Yuan as group;
The 4th step: artificial pollination, select more than 2 years without damage by disease and insect, and possesses the fine individual plant of this kind characteristic feature, early flowering season is chosen white anther cap with toothpick, to expose yellow pollen, with toothpick, dip, invest gently the recess of maternal plant gynoecium, the relevant informations such as the rear listing mark father of pollination, female parent and pollination time;
The 5th step: capsule is gathered, pollination is by 40 to 60 days, when outstanding achievement appearance is started to become when light yellow from green, fruit maturation and cracking is not gathered in time; After gathering, with clean paper handkerchief, wrap, be placed in 5~10 ℃ of refrigerators stand-by, the holding time is unsuitable long;
The 6th step: capsule is processed and inoculation, the capsule of gathering is placed in 75% alcohol and carries out surface sterilization 30 seconds, with sterile water wash once after, be placed in 0.1% mercuric chloride and sterilize 20 minutes, in disinfecting process, want intermittent oscillation, that sterilization is abundant, after finishing, use aseptic water washing 3~5 times, under aseptic condition, fruit is cut, seed is spread on the MS medium that 5-10 bottle configured equably, culture environment is: 24~26 ℃ of temperature, relative moisture 60%, illumination is 1000 luxs, the light application time of every day is 12 hours, through about 50 days, in bottle, grow protocorm, observed and recorded differential growth situation,
The 7th step: protocorm increment is cultivated, the stalwartness that induction is produced, growth color normal protocorm carries out cutting to be transferred on MS minimal medium and breed and expand bottle, and medium also needs to add 12% murphy juice and 0.5% methyl α-naphthyl acetate, and storing environment is 24~26 ℃ of temperature, humidity is 60%, illumination is 2000 luxs, and every day, light application time was 14 hours, cultivates 50~60 days, in incubation, observed and recorded upgrowth situation;
The 8th step: strengthening seedling and rooting is cultivated, when the seedling 2~3cm of protocorm differentiation, by the size fractionation of seedling, transplant in MS medium (medium is containing 1% methyl α-naphthyl acetate) and cultivate, transplant 60 strains for every bottle, storing environment is: 24~26 ℃ of temperature, humidity is 60%, illumination is 2500~3000 luxs, illumination every day 18 hours, carries out culture of rootage with this understanding, within 60 days, observes afterwards and records height of seedling, the growing states such as root system number, when seedling reaches 4 more than leaf, more than plant height 3cm, during the above condition of 3 of roots, without carrying out hardening, can carry out outdoor planting;
The 9th step: bottle outlet, medium is taken out gently together with seedling, be placed in large basin and clean up with clear water, suitably classification is placed in 1000 times of liquid of 70% potassium permanganate and soaks seedling 5~10 minutes, is placed in to dry under scattered light to wait to plant to the root deliquescing of turning white for 5~7 hours;
The tenth step: plantation, the weather in Guangdong Province of take is standard, and in annual March~all can transplant by the end of November, best plantation period is spring, Qiu Liangji, clump is planted, take 3 to 4 strains as one clump, by the plantation of 8cm * 10cm line-spacing, during plantation, will handle with care, do not break fleshy root as far as possible, planting depth is 2~3cm, and different brackets seedling is plantation separately, convenient management; According to different seedbeds pattern, it is 2.4 ten thousand clumps of left and right that every mu of booth is planted seedling quantity;
The 11 step: seedling management, transplant 7~15 days seedling initial stages, atomized water spray in canopy, keeps the interior humidity of canopy in 80% left and right, available 80 object sunshade net sunshades; Transplant 15~30 days, seedling grows new root, starts to send out sprouting, and the drenched matrix of watering is advisable, and in canopy, relative air humidity is advisable with 70~80%, available 70 order sunshade net sunshades, and note ventilating, now can execute slow-release fertilizer or organic fertilizer that N:P:K is 1:1:1; After 11~30 days, seedling starts to enter fast growing period, can spray composite fertilizer or the organic fertilizer of 1 1:1500 times of liquid in conjunction with watering at every turn, and cup is planted seedling and within general 1~2 day, drenched a water; Bed is planted seedling can drench a water in 2~5 days according to the moistening degree of matrix, can drench matrix top layer 2cm left and right for degree;
The 12 step: become seedling management in age,
During spring in 3~May, outside air temperature gos up gradually, air is more moistening, plant starts growth, basal part of stem is sprouted sprouting, grows new root, and now seedling increases gradually to liquid manure demand, a water of every mornings 7 point~10 spray during fine day, wetting stromal surface, and can be in conjunction with trickle, each pouring executed liquid manure one time, dilute 1000 times of liquid, within 10~15 days, spray a sterilization (worm) agent, full exposure, suitable shading when noon, sunlight was fierce, in canopy, keep ventilated, " going back to Nan Tian ", will carry out mandatory ventilation;
During summer in 6~August, weather is hot and humid, it is dendrobium candidum vigorous growth season, also be cultivation management important period, temperature is many 30 ℃ of left and right, this period is sunny, should avoid sunlight directly to shine, easily there is " sunscald " in carelessness slightly, every day, 11:00-16:00 canopy planted agent started two net shadings under normal circumstances, shading 30-50%, and improve ventilation condition in canopy, in hot weather plant body, water consumption is larger, should supplement in time, every day is except morning normal trickle, afternoon, post sunset increased pouring once, from 5-9 month, every two weeks is executed disposable fertilizer, depending on Changes in weather situation, determine liquid manure consumption, temperature is 35 ℃ of left and right, want discontinuity to sprinkle water earthward, with high humidity, lower the temperature, note leaf spot and soft rot, diseased plant is removed in time in discovery, avoid in high temperature and the fertilising of high light time,
During autumn in 9~November, temperature declines gradually, day and night temperature is about 5~10 ℃, temperature now and temperature range are extremely conducive to effective accumulation of organic principle in plant body, top grows termination leaf during this time, plant is from nourishing and growing toward reproductive growth transition, pseudobulb increases the thick increase stage, now should increase intensity of illumination in canopy, shorten the shading time, sufficient illumination is conducive to generation and the accumulation of nutriment, still likely there is high temperature in weather now, note also according to weather condition adjustment and ventilate, cooling, guaranteeing that matrix is not under the prerequisite of ponding, keep moistening, avoid the long-term overdrying of matrix, otherwise be also unfavorable for plant strain growth, regularly carry out artificial weeding and loose matrix, the insect pest of finding is carried out to artificial capture method,
12~February is during winter approached, progress into hypothermic phase, now plant strain growth is slowly to stopping growing, in stem stalk body, content of organics reaches maximum, flower bud differentiation completes, and part stipes slowly completes bud elongation, inflorescence and flower bud development until bloom, and plant grows and slows down in the time of 10~15 ℃, in the time of≤10 ℃, stop growing, can resist the microthermal climate of short-term≤5 ℃; Now full exposure in canopy, reduces trickle number of times in the time of≤10 ℃, within 7~10 days, is once advisable, and tries not to pour blade during operation, within 10~15 days, drenches once balanced fertile (2000 times of water liquid);
The 13 step: gather, suitable collecting time is June November to next year, adopts the old new and herb 2 kinds of modes of gathering of staying, carry out adopt old stay new, 20 months acrial part plant in vegetative period above of gathering;
The 14 step: check and accept and storage, after gathering, reject in time diseased plant, according to related standards such as < < Pharmacopoeia of People's Republic of China > >, company standard and purchase orders, detect the projects such as polysaccharide, moisture, agricultural chemicals, heavy-metal residual; Iron-sheet dendrobe fresh product is placed in shady and cool moist place;
The 15 step: fresh goods by removal of impurities, clean after low temperature drying at segment, 60 ℃, water content is no more than 12%, dry product is placed in aeration-drying place, protection against the tide.
2. dendrobium candidum implantation methods as claimed in claim 1, is characterized in that: in described the 8th step, according to seedling size, be divided into qualified clusters training seedling and high-quality seedling, described qualified clusters training seedling standard: 2 to 5 of roots, and 4 leaves, plant height >=2.5cm, stem is thick >=0.2cm; Described high-quality seedling standard: 3 to 5 of roots, 6 leaves, plant height >=4.0cm, stem is thick >=0.3cm.
3. dendrobium candidum implantation methods as claimed in claim 1, is characterized in that: when described the tenth step, need to use inorganic fertilizer or organic fertilizer to apply fertilizer.
4. dendrobium candidum implantation methods as claimed in claim 1, it is characterized in that: in described the tenth step, should be noted that and in canopy, have higher humidity and weak illumination, matrix is not dry does not water, so that inducing rooting, field planting can be sprayed low concentration tpn after 3 days carries out prevention of damage by disease.
CN201410284005.1A 2014-06-24 2014-06-24 Dendrobium officinale planting method Pending CN104025987A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410284005.1A CN104025987A (en) 2014-06-24 2014-06-24 Dendrobium officinale planting method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410284005.1A CN104025987A (en) 2014-06-24 2014-06-24 Dendrobium officinale planting method

Publications (1)

Publication Number Publication Date
CN104025987A true CN104025987A (en) 2014-09-10

Family

ID=51457154

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410284005.1A Pending CN104025987A (en) 2014-06-24 2014-06-24 Dendrobium officinale planting method

Country Status (1)

Country Link
CN (1) CN104025987A (en)

Cited By (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104488706A (en) * 2014-12-03 2015-04-08 柳州市泓吉农业科技有限公司 Dendrobe officinale transplanting method
CN104521469A (en) * 2014-12-03 2015-04-22 柳州市泓吉农业科技有限公司 Dendrobium soft rot controlling methods
CN104686308A (en) * 2015-02-11 2015-06-10 贵州绿春缘有机农业产业管理有限公司 Cultivation method of Dendrobium officinale Kimura et Migo
CN104686207A (en) * 2015-03-25 2015-06-10 浙江铁枫堂科技股份有限公司 Method for cultivating dendrobium officinale
CN104938316A (en) * 2015-06-29 2015-09-30 广西健宝石斛有限责任公司 Cultivation method for dendrobium officinale
CN104938315A (en) * 2015-06-29 2015-09-30 广西健宝石斛有限责任公司 Planting method for dendrobium officinale
CN104996286A (en) * 2015-07-03 2015-10-28 浙江仙人草保健品有限公司 Method for planting dendrobium candidum by adopting multi-layered three-dimensional cultivating rack
CN105010108A (en) * 2015-07-03 2015-11-04 浙江仙人草保健品有限公司 Method for planting dendrobium officinale by using net-structured seedbed
CN105010110A (en) * 2015-07-03 2015-11-04 浙江仙人草保健品有限公司 Method for planting dendrobium officinale by use of inner concave type seedbed
CN105075819A (en) * 2015-06-30 2015-11-25 广西健宝石斛有限责任公司 Household planting method of Dendrobium officinale
CN105075820A (en) * 2015-07-03 2015-11-25 浙江仙人草保健品有限公司 Method for planting dendrobium officinale by adopting tilt-type seedbed
CN105145296A (en) * 2015-07-03 2015-12-16 浙江仙人草保健品有限公司 Method for planting dendrobium candidum by adopting hydrophilic polyurethane foam
CN105248265A (en) * 2015-11-04 2016-01-20 富阳市文曲生态农业开发有限公司 Method of planting dendrobium officinale by using moso bamboo
CN105393741A (en) * 2015-10-26 2016-03-16 安徽霍山县药王谷林农科技有限公司 Breeding method for further optimizing wild improved variety of dendrobium huoshanense
CN106035025A (en) * 2016-06-22 2016-10-26 浙江高鼻子生物科技有限公司 Rock wall cultivation method for Dendrobium officinale
CN106034673A (en) * 2016-06-14 2016-10-26 福建省农业科学院植物保护研究所 Simple pest control method for Dendrobium officinale greenhouse planting
CN106550862A (en) * 2016-11-15 2017-04-05 广西壮族自治区防城金花茶国家级自然保护区管理处 The candidum tissue culturing seedling for exempting from seedling exercising ties up tree new method for cultivating
CN106561204A (en) * 2016-09-30 2017-04-19 南京仙草堂生物科技有限公司 Method for improving alcohol-soluble extractive content of dendrobium officinale kimura et migo
CN106576551A (en) * 2016-12-23 2017-04-26 江苏农林职业技术学院 Fertilizing method for improving polysaccharide content of dendrobium officinale
CN107018903A (en) * 2017-05-09 2017-08-08 贵州济生农业科技有限公司 A kind of implantation methods of dendrobium candidum
CN107517838A (en) * 2017-10-10 2017-12-29 江西轩斛生物科技有限公司 A kind of dendrobium candidum gemmule method for culturing seedlings
CN107950342A (en) * 2017-11-02 2018-04-24 莫玉艳 A kind of stem of noble dendrobium greenhouse gardening method
CN108293863A (en) * 2018-02-11 2018-07-20 绍兴儒林生物科技有限公司 A kind of dendrobium candidum ecosystem is from the implantation methods that germinate
CN108323430A (en) * 2017-12-25 2018-07-27 安徽霍山县松鹤堂中药材有限公司 A kind of wild Dendrodium transplanting mating system
CN109220793A (en) * 2018-09-28 2019-01-18 山东省农业科学院蔬菜花卉研究所 A kind of selection of iris new varieties
CN109997622A (en) * 2019-04-17 2019-07-12 广州市八斗农业科技有限公司 The method for improving dendrobium candidum active constituent content
CN110100737A (en) * 2019-06-21 2019-08-09 台州市椒江草之堂生物科技有限公司 A kind of candidum tissue culturing rapid propagation method
CN110187052A (en) * 2019-03-08 2019-08-30 合肥学院 It is a kind of based on the plant-growth factor of cloud computing to the analysis method of its growth effect
CN112703975A (en) * 2020-12-01 2021-04-27 贵州省利增农业旅游发展有限公司 Artificial planting method for dendrobium officinale
CN114963745A (en) * 2022-05-23 2022-08-30 舟山市食品药品检验检测研究院 Optimized processing technology for dendrobium officinale produced in Zhejiang

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
佚名: "[农广天地]铁皮石斛温室大棚种植技术(20120416)_农家乐_中国网络电视台", 《百度文库HTTP://WENKU.BAIDU.COM/LINK?URL=45DH5LLJOJKEW5_ZSP7ZWLWLZFUG3TE7XWVKPHJFOVR9TQUEUPTWRWCN3T9_3FIX41LXVZJLDYTYOAW4Q6IKQVBKHHPY1ZD24N_J6TENDOE》 *
张志勇: "铁皮石斛人工杂交育种快繁技术", 《福建农业科技》 *
蒙平 等: "广西铁皮石斛规范化生产标准操作规程", 《广东农业科学》 *
郑平 等: "广东地区铁皮石斛的特征特性及繁殖栽培管理", 《广东农业科学》 *

Cited By (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104521469A (en) * 2014-12-03 2015-04-22 柳州市泓吉农业科技有限公司 Dendrobium soft rot controlling methods
CN104488706A (en) * 2014-12-03 2015-04-08 柳州市泓吉农业科技有限公司 Dendrobe officinale transplanting method
CN104686308A (en) * 2015-02-11 2015-06-10 贵州绿春缘有机农业产业管理有限公司 Cultivation method of Dendrobium officinale Kimura et Migo
CN104686207A (en) * 2015-03-25 2015-06-10 浙江铁枫堂科技股份有限公司 Method for cultivating dendrobium officinale
CN104938316A (en) * 2015-06-29 2015-09-30 广西健宝石斛有限责任公司 Cultivation method for dendrobium officinale
CN104938315A (en) * 2015-06-29 2015-09-30 广西健宝石斛有限责任公司 Planting method for dendrobium officinale
CN105075819A (en) * 2015-06-30 2015-11-25 广西健宝石斛有限责任公司 Household planting method of Dendrobium officinale
CN105010110A (en) * 2015-07-03 2015-11-04 浙江仙人草保健品有限公司 Method for planting dendrobium officinale by use of inner concave type seedbed
CN105010108A (en) * 2015-07-03 2015-11-04 浙江仙人草保健品有限公司 Method for planting dendrobium officinale by using net-structured seedbed
CN105075820A (en) * 2015-07-03 2015-11-25 浙江仙人草保健品有限公司 Method for planting dendrobium officinale by adopting tilt-type seedbed
CN105145296A (en) * 2015-07-03 2015-12-16 浙江仙人草保健品有限公司 Method for planting dendrobium candidum by adopting hydrophilic polyurethane foam
CN104996286A (en) * 2015-07-03 2015-10-28 浙江仙人草保健品有限公司 Method for planting dendrobium candidum by adopting multi-layered three-dimensional cultivating rack
CN105393741A (en) * 2015-10-26 2016-03-16 安徽霍山县药王谷林农科技有限公司 Breeding method for further optimizing wild improved variety of dendrobium huoshanense
CN105248265A (en) * 2015-11-04 2016-01-20 富阳市文曲生态农业开发有限公司 Method of planting dendrobium officinale by using moso bamboo
CN106034673B (en) * 2016-06-14 2018-11-30 福建省农业科学院植物保护研究所 A kind of simplification pest controlling method for dendrobium candidum greenhouse gardening
CN106034673A (en) * 2016-06-14 2016-10-26 福建省农业科学院植物保护研究所 Simple pest control method for Dendrobium officinale greenhouse planting
CN106035025A (en) * 2016-06-22 2016-10-26 浙江高鼻子生物科技有限公司 Rock wall cultivation method for Dendrobium officinale
CN106561204A (en) * 2016-09-30 2017-04-19 南京仙草堂生物科技有限公司 Method for improving alcohol-soluble extractive content of dendrobium officinale kimura et migo
CN106550862A (en) * 2016-11-15 2017-04-05 广西壮族自治区防城金花茶国家级自然保护区管理处 The candidum tissue culturing seedling for exempting from seedling exercising ties up tree new method for cultivating
CN106576551A (en) * 2016-12-23 2017-04-26 江苏农林职业技术学院 Fertilizing method for improving polysaccharide content of dendrobium officinale
CN107018903A (en) * 2017-05-09 2017-08-08 贵州济生农业科技有限公司 A kind of implantation methods of dendrobium candidum
CN107517838A (en) * 2017-10-10 2017-12-29 江西轩斛生物科技有限公司 A kind of dendrobium candidum gemmule method for culturing seedlings
CN107950342A (en) * 2017-11-02 2018-04-24 莫玉艳 A kind of stem of noble dendrobium greenhouse gardening method
CN108323430A (en) * 2017-12-25 2018-07-27 安徽霍山县松鹤堂中药材有限公司 A kind of wild Dendrodium transplanting mating system
CN108293863A (en) * 2018-02-11 2018-07-20 绍兴儒林生物科技有限公司 A kind of dendrobium candidum ecosystem is from the implantation methods that germinate
CN109220793A (en) * 2018-09-28 2019-01-18 山东省农业科学院蔬菜花卉研究所 A kind of selection of iris new varieties
CN110187052A (en) * 2019-03-08 2019-08-30 合肥学院 It is a kind of based on the plant-growth factor of cloud computing to the analysis method of its growth effect
CN109997622A (en) * 2019-04-17 2019-07-12 广州市八斗农业科技有限公司 The method for improving dendrobium candidum active constituent content
CN110100737A (en) * 2019-06-21 2019-08-09 台州市椒江草之堂生物科技有限公司 A kind of candidum tissue culturing rapid propagation method
CN112703975A (en) * 2020-12-01 2021-04-27 贵州省利增农业旅游发展有限公司 Artificial planting method for dendrobium officinale
CN114963745A (en) * 2022-05-23 2022-08-30 舟山市食品药品检验检测研究院 Optimized processing technology for dendrobium officinale produced in Zhejiang

Similar Documents

Publication Publication Date Title
CN104025987A (en) Dendrobium officinale planting method
CN102318547B (en) Overhead cultivation method of Dendrobium huoshanense test-tube seedlings
CN103918536B (en) The implantation methods of dendrobium candidum
CN103999672B (en) A kind of method of non-polluted planting dendrobium candidum
CN103004445B (en) Method for directly seedling dendrobe seedlings by using plastic greenhouse
CN103548652B (en) Rapid tree cultivation method for dendrobium candidum tissue-culture bottle seedlings
CN104521715B (en) Sealing water planting cutting propagation method for gardenias with flower buds
CN103766113B (en) Method for planting anoectochilus formosanus in northern area
CN105191763A (en) Vegetable organic soil-less culture method, culture medium and manufacturing method thereof
CN103907467B (en) A kind of breeding method of beech tree high quality seedling
Hernández et al. Cultivation systems
CN102792838A (en) Greenhouse cultivation method for anoectochilus formosanus
CN103798049A (en) Lonicera japonica twig cutting cultivation method
CN104663422B (en) A kind of producing method for seed of hybrid rice
CN103081675A (en) Method of cultivating eustoma grandiflorum flowering plant seedlings
CN103891493A (en) Two-section type high-yield cultivation method for greenhouse asparagus
CN105918051A (en) Greenhouse cultivation method for nectarines
CN103444530A (en) Technology for field return-to-nature ex-situ plantation of wild anoectochilus roxburghii (Wall.) Lindl in Fujian
CN104521705A (en) Cultivating and conserving method for facility strawberries
CN101627729B (en) High-quality corm of colorful calla and method for cultivating same
CN104956869A (en) Cotton seedling nursing and transplanting method
CN103960030A (en) Method for cultivating anoectochilus formosanus in wild imitation mode
CN106069535A (en) A kind of method improving seed of Fructus Vitis viniferae planting percent
CN108157098A (en) A kind of acclimatization and transplants method of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling
CN104303765B (en) The high-yield planting method of the stem of noble dendrobium

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20140910