CN105248262A - Pine bark-biochar matrix for culture of Dendrobium officinale - Google Patents

Pine bark-biochar matrix for culture of Dendrobium officinale Download PDF

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Publication number
CN105248262A
CN105248262A CN201510645725.0A CN201510645725A CN105248262A CN 105248262 A CN105248262 A CN 105248262A CN 201510645725 A CN201510645725 A CN 201510645725A CN 105248262 A CN105248262 A CN 105248262A
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charcoal
pine bark
dendrobium candidum
matrix
dendrobium officinale
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CN105248262B (en
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吕豪豪
杨生茂
刘玉学
汪玉瑛
钟哲科
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Zhejiang Academy of Agricultural Sciences
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Zhejiang Academy of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a pine bark-biochar matrix for culture of Dendrobium officinale. The pine bark-biochar matrix is prepared by mixing the following components in mass percentage: 56-67% of pine bark, 24-28% of peat and 5-20% of biochar. The pine bark-biochar matrix can substantially improve the contents of microelements necessary to a human body such as Zn, Co and the like in the Dendrobium officinale, can remarkably improve the polysaccharide content of the Dendrobium officinale, facilitates adsorption of potassium, and obviously improves the appearance and quality of the cultivated Dendrobium officinale.

Description

A kind of pine bark for officinal dendrobium stem plantation-charcoal matrix
Technical field
The present invention relates to a kind of dendrobium officinale culture medium, particularly a kind of pine bark for officinal dendrobium stem plantation-charcoal matrix.
Background technology
Dendrobium candidum, also known as ribbed hedyotis herb, belonging to the orchid family epiphytic orchid class, is traditional rare traditional Chinese medicine, is among the peoplely described as " first of Chinese nine immortal grass ".Be listed in top grade in Shennong's Herbal, effects such as there is nourishing Yin and clearing heat, the beneficial stomach that promotes the production of body fluid, relieving cough and moistening lung, the improving eyesight that wets one's whistle, improve human immunity, anti-curing oncoma and angiocardiopathy and improving water flood, prevent and treat cataract, promote longevity.Dendrobium candidum to growing environment and weather conditional request very harsh.For many years, dendrobium candidum crude drug source depends on wild resource, long-term excessive collection, makes wild resource exhausted and is difficult to meet the need of market, therefore needing artificial cultivation dendrobium candidum to meet the need of market.
At present, the artificial planting area of China's dendrobium candidum increases gradually, but the market demand is still in continuous growth.Through developmental research for many years, dendrobium candidum obtains many achievements in large-scale planting, but how to improve the problem that dendrobium candidum yield and quality is still the continuous research of needs.In dendrobium candidum plantation, the matrix such as peat, pine bark, stone is obtained extensive use, mud and charcoal worsening shortages is also there is in actual production, pine bark, stone plantation Growth of Dendrobium candidum is slow, matrix noxious material is more, the problem such as ventilative water permeability reduction after substrate degradation corruption, therefore, base material improvement and the exploitation of new cultivation matrix are taken seriously gradually.Charcoal is being improved in soil and Promoting plant growth by people's extensive concern, but the effect of charcoal in soilless culture substrate and influencing each other also is known little about it, and rarely has report especially after adding charcoal to original matrix on the growth of dendrobium candidum and the impact of quality.
Summary of the invention
The object of the present invention is to provide a kind of pine bark for officinal dendrobium stem plantation-charcoal matrix, the content of micro elements needed by human Zn, Co etc. in dendrobium candidum can be significantly improved, and can polysaccharide content of dendrobium candidum be significantly improved, promote the absorption of potassium, make the dendrobium candidum of cultivating all have obvious lifting in outward appearance and quality.
The technical solution adopted for the present invention to solve the technical problems is:
For pine bark-charcoal matrix of officinal dendrobium stem plantation, be mixed by the component of following mass percent: pine bark 56-67%, peat 24-28%, charcoal 5-20%.
The present invention improves dendrobium officinale culture medium, add charcoal and improve formula, charcoal has good air capacity of soils, preserve moisture and fertility, habitat can be provided for growth of microorganism, promote the breeding of microorganism, microorganism amount reproduction, can promote to utilize the decomposition of the nutrient component in pine bark, peat, thus promote Growth of Dendrobium candidum.Add charcoal act on especially be to significantly improve micro elements needed by human Zn in dendrobium candidum, the content of Co etc., promote the absorption of potassium, and polysaccharide content of dendrobium candidum can be significantly improved, thus improve dendrobium candidum quality.
Peat does not decompose the product of accumulation completely as the residual body of limnocryptophyte, and its content of organic matter has exceeded 50% (with dry basis).The pH of peat, about 5.5 ~ 6.5, is rich in humus, nutritious, total porosity comparatively large (79% ~ 98%), the less (0.04 ~ 0.27g/cm of unit weight 3), and there is good water conservation and nutrient preserving capability, metastable physicochemical property.
As preferably, described charcoal is mixed according to the weight proportion of 1:1:3 by bamboo charcoal, bagasse charcoal and pecan shell charcoal.
The present invention combines with the charcoal of particular types, especially the charcoal that the discarded object of this Hangzhou local characteristic of pecan shell charcoal is processed is selected, the combination of these three kinds of charcoals, comprehensive onset, the hole of charcoal formed, specific surface area and contained nutrient component and trace element are more suitable for the production of dendrobium candidum, improve the quality of dendrobium candidum.
As preferably, the preparation method of described bamboo charcoal is: by bamboo in oxygen-free environment, cracking 3 hours at 400 DEG C and obtaining.
As preferably, the preparation method of described bagasse charcoal is: by bagasse in oxygen-free environment, cracking 3 hours at 400 DEG C and obtaining.
As preferably, the preparation method of described pecan shell charcoal is: by pecan shell in oxygen-free environment, cracking 3 hours at 400 DEG C and obtaining.
The charing of bamboo charcoal of the present invention, bagasse charcoal, pecan shell charcoal is carried out in oxygen-free environment, and adopt the preparation of low-temperature carbonization technology, charcoal pH value lower (about 7) under cryogenic, be conducive to the plantation of dendrobium candidum, specific surface area is greater than 80m 2/ g, result of use is good.
As preferably, the particle diameter of described charcoal is 2-5mm.
As preferably, the specification of described pine bark is: particle diameter 12-20mm.Control the specification of pine bark, meet the requirement of Growth of Dendrobium candidum to air capacity of soils, be more suitable for the production of dendrobium candidum.
The invention has the beneficial effects as follows: the content that can significantly improve micro elements needed by human Zn, Co etc. in dendrobium candidum, and can polysaccharide content of dendrobium candidum be significantly improved, promote the absorption of potassium, make the dendrobium candidum of cultivating all have obvious lifting in outward appearance and quality.
Accompanying drawing explanation
Fig. 1 be in matrix different charcoal addition to the effect diagram of polysaccharide content of dendrobium candidum.
Fig. 2 is full potassium content variation diagram in the dendrobium candidum plant corpus after different substrates is cultivated.
Embodiment
Below by specific embodiment, and by reference to the accompanying drawings, technical scheme of the present invention is described in further detail.
In the present invention, if not refer in particular to, the raw material adopted and equipment etc. all can be buied from market or this area is conventional.Method in following embodiment, if no special instructions, is the conventional method of this area.
Embodiment 1:
For pine bark-charcoal matrix of officinal dendrobium stem plantation, be mixed by the component of following mass percent: pine bark (commercially available, particle diameter 12-20mm) 56%, peat 24%, charcoal (particle diameter is 2-5mm) 20%.
Described charcoal is mixed according to the weight proportion of 1:1:3 by bamboo charcoal, bagasse charcoal and pecan shell charcoal.The preparation method of described bamboo charcoal is: by bamboo in oxygen-free environment, cracking 3 hours at 400 DEG C and obtaining.The preparation method of described bagasse charcoal is: by bagasse in oxygen-free environment, cracking 3 hours at 400 DEG C and obtaining.The preparation method of described pecan shell charcoal is: by pecan shell in oxygen-free environment, cracking 3 hours at 400 DEG C and obtaining.
Embodiment 2:
A kind of pine bark for officinal dendrobium stem plantation-charcoal matrix, be mixed by the component of following mass percent: pine bark is (commercially available, particle diameter 12-20mm) 67%, peat (commercially available) 28%, charcoal (particle diameter is 2-5mm) 5%.
Described charcoal is mixed according to the weight proportion of 1:1:3 by bamboo charcoal, bagasse charcoal and pecan shell charcoal.The preparation method of described bamboo charcoal is: by bamboo in oxygen-free environment, cracking 3 hours at 400 DEG C and obtaining.The preparation method of described bagasse charcoal is: by bagasse in oxygen-free environment, cracking 3 hours at 400 DEG C and obtaining.The preparation method of described pecan shell charcoal is: by pecan shell in oxygen-free environment, cracking 3 hours at 400 DEG C and obtaining.
Embodiment 3:
For pine bark-charcoal matrix of officinal dendrobium stem plantation, be mixed by the component of following mass percent: pine bark (commercially available, particle diameter 12-20mm) 63%, peat 27%, charcoal (particle diameter is 2-5mm) 10%.
Described charcoal is mixed according to the weight proportion of 1:1:3 by bamboo charcoal, bagasse charcoal and pecan shell charcoal.The preparation method of described bamboo charcoal is: by bamboo in oxygen-free environment, cracking 3 hours at 400 DEG C and obtaining.The preparation method of described bagasse charcoal is: by bagasse in oxygen-free environment, cracking 3 hours at 400 DEG C and obtaining.The preparation method of described pecan shell charcoal is: by pecan shell in oxygen-free environment, cracking 3 hours at 400 DEG C and obtaining.
The mensuration of dendrobium candidum nutrient and quality
(1) determination of trace element: micro-wave digestion-Inductively coupled plasma atomic emission sperctrometry method (ICP-AES).
(2) dendrobium polysaccharide measures: phend-sulphuric acid.
Test establishes 4 to process (see table 1, CK1 is control group, SNB1-SNB3 is the matrix of embodiment of the present invention 1-3), adopt completely randomized design, use different substrates cultivation of dendrobium officinale, every basin two strain dendrobium candidum seedling, every one, 8 basin process, each process repetition 12 times, cultivates and measures after 280 days.
Process Pine bark (%) Peat (%) Charcoal (%)
CK1 70 30 0
SNB1 67 28 5
SNB2 63 27 10
SNB3 56 24 20
After testing, in the dendrobium candidum respectively organized, micro elements needed by human content is in table 2.As shown in Table 2, in the dendrobium candidum that the matrix (SNB1-SNB3) after interpolation charcoal is cultivated, the content of micro elements needed by human Zn and Co is significantly higher than control group (CK1), and the content of Fe also improves relative to control group.
Table 2 (mg/kg)
Note: in table, the different lowercase of same column represents that P < 0.05 level difference is remarkable, and in table, data are 3 mean values.
Polysaccharide is one of dendrobium candidum main active, has close contacting with the drug effect of dendrobium candidum, is the important indicator weighing dendrobium candidum quality." Chinese Pharmacopoeia " (2010 editions) regulation Dendrobium officinale polysaccharide is with DEXTROSE ANHYDROUS (C 6h 12o 6) meter, must not 25.0% be less than.Can be known by Fig. 1, each process polyoses content, all more than 25.0%, meets national dendrobium candidum quality standard.In SNB3, SNB2 and SNB1, be respectively 36.40%, 35.70% and 35.42%; That content is minimum is CK1, is 32.16%.Compared with CK1, along with the increase of charcoal addition, polysaccharide content of dendrobium candidum increases gradually, and SNB1, SNB2 and SNB3 and CK1 all have significant difference.
As shown in Figure 2, after transplanting 280d, in dendrobium candidum plant corpus, full potassium content is maximum in SNB1, is secondly that lower is CK1 in SNB2 and SNB3.The visible charcoal that adds in pine bark and peat mixed-matrix promotes that dendrobium candidum is to the absorption of matrix potassium, and when addition is 5%, best results.
Above-described embodiment is one of the present invention preferably scheme, not does any pro forma restriction to the present invention, also has other variant and remodeling under the prerequisite not exceeding the technical scheme described in claim.

Claims (7)

1., for pine bark-charcoal matrix of officinal dendrobium stem plantation, it is characterized in that, be mixed by the component of following mass percent: pine bark 56-67%, peat 24-28%, charcoal 5-20%.
2. a kind of pine bark for officinal dendrobium stem plantation-charcoal matrix according to claim 1, is characterized in that: described charcoal is mixed according to the weight proportion of 1:1:3 by bamboo charcoal, bagasse charcoal and pecan shell charcoal.
3. a kind of pine bark for officinal dendrobium stem plantation-charcoal matrix according to claim 2, is characterized in that: the preparation method of described bamboo charcoal is: by bamboo in oxygen-free environment, cracking 3 hours at 400 DEG C and obtaining.
4. a kind of pine bark for officinal dendrobium stem plantation-charcoal matrix according to claim 2, is characterized in that: the preparation method of described bagasse charcoal is: by bagasse in oxygen-free environment, cracking 3 hours at 400 DEG C and obtaining.
5. a kind of pine bark for officinal dendrobium stem plantation-charcoal matrix according to claim 2, is characterized in that: the preparation method of described pecan shell charcoal is: by pecan shell in oxygen-free environment, cracking 3 hours at 400 DEG C and obtaining.
6. a kind of pine bark for officinal dendrobium stem plantation-charcoal matrix according to claim 1 and 2, is characterized in that: the particle diameter of described charcoal is 2-5mm.
7. a kind of pine bark for officinal dendrobium stem plantation-charcoal matrix according to claim 1 and 2, is characterized in that: the specification of described pine bark is: particle diameter 12-20mm.
CN201510645725.0A 2015-10-08 2015-10-08 A kind of pine bark charcoal matrix for officinal dendrobium stem plantation Active CN105248262B (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106035028A (en) * 2016-06-30 2016-10-26 上海孙桥农业科技股份有限公司 Cultivation substrate for dendrobium officinale and preparation method
CN108739246A (en) * 2018-05-05 2018-11-06 安徽霍山斛花郎石斛开发有限公司 A kind of cultivation matrix improving dendrobium candidum quality

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104072305A (en) * 2014-07-18 2014-10-01 时科生物科技(上海)有限公司 Special bamboo charcoal bio-organic fertilizer for dendrobe and production method for bamboo charcoal bio-organic fertilizer
CN104604660A (en) * 2015-02-11 2015-05-13 贵州绿春缘有机农业产业管理有限公司 Imitating wild condition planting method of dendrobium officinale
CN104686308A (en) * 2015-02-11 2015-06-10 贵州绿春缘有机农业产业管理有限公司 Cultivation method of Dendrobium officinale Kimura et Migo

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104072305A (en) * 2014-07-18 2014-10-01 时科生物科技(上海)有限公司 Special bamboo charcoal bio-organic fertilizer for dendrobe and production method for bamboo charcoal bio-organic fertilizer
CN104604660A (en) * 2015-02-11 2015-05-13 贵州绿春缘有机农业产业管理有限公司 Imitating wild condition planting method of dendrobium officinale
CN104686308A (en) * 2015-02-11 2015-06-10 贵州绿春缘有机农业产业管理有限公司 Cultivation method of Dendrobium officinale Kimura et Migo

Non-Patent Citations (1)

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Title
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106035028A (en) * 2016-06-30 2016-10-26 上海孙桥农业科技股份有限公司 Cultivation substrate for dendrobium officinale and preparation method
CN108739246A (en) * 2018-05-05 2018-11-06 安徽霍山斛花郎石斛开发有限公司 A kind of cultivation matrix improving dendrobium candidum quality
CN108739246B (en) * 2018-05-05 2020-09-29 安徽霍山斛花郎石斛开发有限公司 Culture medium for improving quality of dendrobium officinale

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