CN108739246B - Culture medium for improving quality of dendrobium officinale - Google Patents
Culture medium for improving quality of dendrobium officinale Download PDFInfo
- Publication number
- CN108739246B CN108739246B CN201810422777.5A CN201810422777A CN108739246B CN 108739246 B CN108739246 B CN 108739246B CN 201810422777 A CN201810422777 A CN 201810422777A CN 108739246 B CN108739246 B CN 108739246B
- Authority
- CN
- China
- Prior art keywords
- parts
- dendrobium officinale
- mixture
- hours
- weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241001076416 Dendrobium tosaense Species 0.000 title claims abstract description 38
- 239000001963 growth medium Substances 0.000 title claims description 9
- 239000002131 composite material Substances 0.000 claims abstract description 37
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims abstract description 36
- 235000002566 Capsicum Nutrition 0.000 claims abstract description 30
- 239000006002 Pepper Substances 0.000 claims abstract description 30
- 235000016761 Piper aduncum Nutrition 0.000 claims abstract description 30
- 235000017804 Piper guineense Nutrition 0.000 claims abstract description 30
- 235000008184 Piper nigrum Nutrition 0.000 claims abstract description 30
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical class O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 claims abstract description 28
- 239000000463 material Substances 0.000 claims abstract description 22
- 229910000019 calcium carbonate Inorganic materials 0.000 claims abstract description 18
- 239000002994 raw material Substances 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 238000000855 fermentation Methods 0.000 claims abstract description 7
- 230000004151 fermentation Effects 0.000 claims abstract description 7
- 235000013162 Cocos nucifera Nutrition 0.000 claims abstract description 6
- 244000060011 Cocos nucifera Species 0.000 claims abstract description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000010903 husk Substances 0.000 claims abstract description 6
- 239000003610 charcoal Substances 0.000 claims abstract 2
- 239000000203 mixture Substances 0.000 claims description 35
- 241000722363 Piper Species 0.000 claims description 29
- 238000002360 preparation method Methods 0.000 claims description 19
- 241000255789 Bombyx mori Species 0.000 claims description 18
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 15
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- 239000002245 particle Substances 0.000 claims description 15
- 239000005995 Aluminium silicate Substances 0.000 claims description 14
- 235000012211 aluminium silicate Nutrition 0.000 claims description 14
- 238000010438 heat treatment Methods 0.000 claims description 12
- 239000002893 slag Substances 0.000 claims description 11
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 10
- 229910052782 aluminium Inorganic materials 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 10
- 238000001035 drying Methods 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 9
- 239000000758 substrate Substances 0.000 claims description 9
- 235000014676 Phragmites communis Nutrition 0.000 claims description 6
- 238000010521 absorption reaction Methods 0.000 claims description 6
- 244000195896 dadap Species 0.000 claims description 6
- 239000011435 rock Substances 0.000 claims description 6
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 5
- 239000005944 Chlorpyrifos Substances 0.000 claims description 5
- 240000007594 Oryza sativa Species 0.000 claims description 5
- 235000007164 Oryza sativa Nutrition 0.000 claims description 5
- 238000001354 calcination Methods 0.000 claims description 5
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 5
- 239000006013 carbendazim Substances 0.000 claims description 5
- SBPBAQFWLVIOKP-UHFFFAOYSA-N chlorpyrifos Chemical compound CCOP(=S)(OCC)OC1=NC(Cl)=C(Cl)C=C1Cl SBPBAQFWLVIOKP-UHFFFAOYSA-N 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- ONCZQWJXONKSMM-UHFFFAOYSA-N dialuminum;disodium;oxygen(2-);silicon(4+);hydrate Chemical compound O.[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[Na+].[Na+].[Al+3].[Al+3].[Si+4].[Si+4].[Si+4].[Si+4] ONCZQWJXONKSMM-UHFFFAOYSA-N 0.000 claims description 5
- 239000000835 fiber Substances 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 238000000227 grinding Methods 0.000 claims description 5
- 229920005610 lignin Polymers 0.000 claims description 5
- 239000011259 mixed solution Substances 0.000 claims description 5
- 239000002985 plastic film Substances 0.000 claims description 5
- 229920006255 plastic film Polymers 0.000 claims description 5
- 239000011148 porous material Substances 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 238000000197 pyrolysis Methods 0.000 claims description 5
- 235000009566 rice Nutrition 0.000 claims description 5
- 238000005070 sampling Methods 0.000 claims description 5
- 238000002791 soaking Methods 0.000 claims description 5
- 229940080314 sodium bentonite Drugs 0.000 claims description 5
- 229910000280 sodium bentonite Inorganic materials 0.000 claims description 5
- 239000000243 solution Substances 0.000 claims description 5
- 230000007480 spreading Effects 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 239000010902 straw Substances 0.000 claims description 5
- 239000010409 thin film Substances 0.000 claims 1
- 238000009423 ventilation Methods 0.000 claims 1
- 239000011159 matrix material Substances 0.000 abstract description 16
- 150000004676 glycans Chemical class 0.000 abstract description 15
- 229920001282 polysaccharide Polymers 0.000 abstract description 15
- 239000005017 polysaccharide Substances 0.000 abstract description 15
- 235000015097 nutrients Nutrition 0.000 abstract description 3
- 238000009825 accumulation Methods 0.000 abstract description 2
- 230000035558 fertility Effects 0.000 abstract description 2
- 239000003337 fertilizer Substances 0.000 abstract description 2
- 230000014759 maintenance of location Effects 0.000 abstract description 2
- 239000002366 mineral element Substances 0.000 abstract description 2
- 244000203593 Piper nigrum Species 0.000 abstract 1
- 230000002195 synergetic effect Effects 0.000 abstract 1
- 230000006872 improvement Effects 0.000 description 5
- 238000000034 method Methods 0.000 description 3
- 235000010204 pine bark Nutrition 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 239000003674 animal food additive Substances 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- YNPNZTXNASCQKK-UHFFFAOYSA-N phenanthrene Chemical compound C1=CC=C2C3=CC=CC=C3C=CC2=C1 YNPNZTXNASCQKK-UHFFFAOYSA-N 0.000 description 2
- QWUWMCYKGHVNAV-UHFFFAOYSA-N 1,2-dihydrostilbene Chemical group C=1C=CC=CC=1CCC1=CC=CC=C1 QWUWMCYKGHVNAV-UHFFFAOYSA-N 0.000 description 1
- 241001523681 Dendrobium Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- -1 fluorenone compound Chemical class 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 229960001031 glucose Drugs 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Soil Sciences (AREA)
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to the technical field of dendrobium officinale cultivation, and particularly relates to a cultivation medium for improving the quality of dendrobium officinale, which comprises the following raw materials in parts by weight: 18-26 parts of pepper branch fermentation material, 4-8 parts of coconut husk, 12-16 parts of composite biochar, 3-5 parts of nano calcium carbonate, 1-3 parts of modified kaolin and 2-6 parts of diatomite. Compared with the prior art, the invention has the following advantages: the composite biochar prepared by the invention is matched with other raw materials, so that the water and fertilizer retention of the matrix are better, the loss of nutrient components in the matrix is reduced, the root system distribution of the culture matrix is influenced, the seeds are easy to germinate in the obtained culture matrix, and the crop root system can effectively absorb water and mineral elements in the matrix; the composite biochar and the modified kaolin can improve the matrix fertility; the nano calcium carbonate and the composite charcoal have a synergistic effect, so that the accumulation of total polysaccharide components in the dendrobium officinale is improved, and the obtained dendrobium officinale has higher top-grade performance and use value.
Description
Technical Field
The invention belongs to the technical field of dendrobium officinale cultivation, and particularly relates to a cultivation medium for improving the quality of dendrobium officinale.
Background
The dendrobium officinale has aerial roots and can absorb nutrients and moisture in the air of a supermarket, and the symbiotic fungi at the roots can fix nitrogen in the air, the dendrobium officinale has stronger barren and drought resistance, and has the functions of reducing blood pressure and improving the immunity of organisms along with the continuous research of people on the dendrobium officinale, the dendrobium officinale contains various components such as alkaloid, bibenzyl, polysaccharide, glycoside, phenanthrene, amino acid, fluorenone compound and the like, wherein the main essential component is polysaccharide which has the functions of enhancing immunity, resisting tumor and the like, can clear redundant free radicals of organisms and delay senility, the dendrobium officinale contains more kinds of polysaccharide, the total polysaccharide content of the common dendrobium officinale is between 30 and 45.89 percent, the best content of the water-soluble polysaccharide and the total polysaccharide is found out that the dendrobium officinale has different harvesting time and different polysaccharide content along with the continuous and deep research; the content of dendrobium officinale polysaccharide is different for different varieties and different growth years; the total polysaccharide content of the artificially cultivated dendrobium officinale is about 23.31 percent, and the total polysaccharide content of the wild dendrobium officinale is similar and is 29.38 percent; the content of polysaccharide in the dendrobium officinale planted on rocks and trees under natural conditions is not much different from that in greenhouse artificially planted by 27.26%, 26.74% and 27.20%, but has a certain difference from wild dendrobium.
Disclosure of Invention
The invention aims to provide a culture medium for improving the quality of dendrobium officinale aiming at the existing problems.
The invention is realized by the following technical scheme: a culture medium for improving quality of dendrobium officinale comprises the following raw materials in parts by weight: 18-26 parts of pepper branch fermentation material, 4-8 parts of coconut husk, 12-16 parts of composite biochar, 3-5 parts of nano calcium carbonate, 1-3 parts of modified kaolin and 2-6 parts of diatomite;
the preparation method of the composite biochar comprises the following steps: mixing silkworm excrement, rice straw, reed powder slag and volcanic rock in a weight ratio of 12-16:6-8:2-4:1-2, putting the mixture into a muffle furnace with a program temperature control for sampling pyrolysis, heating to 350 ℃ by a program of 5 ℃/min, preserving heat for 1.5-2.5 hours, continuing heating to 600 ℃ by a program of 10 ℃/min after the completion, preserving heat for 40-60 minutes, naturally cooling to room temperature, taking out, and grinding until the particle size is 0.8-2 mm;
the preparation method of the pepper branch fermented material comprises the following steps: crushing pepper branches into particles with the particle size of 8-10mm, adding fine sawdust accounting for 10-14% of the weight of the pepper branches and erythrina bark accounting for 3-5% of the weight of the pepper branches, uniformly mixing, soaking the mixture for 2-4 hours by using an acetic acid solution with the mass concentration of 2-4%, stacking the mixture in a greenhouse, covering the mixture by using a plastic film, fermenting the mixture for 12-16 hours at the temperature of 35-40 ℃, and naturally spreading and drying the mixture for 4-6 hours; and (3) before transplanting, sterilizing the fermented material by using a mixed solution of 50% of carbendazim and 45% of chlorpyrifos to obtain the pepper branch fermented material.
As a further improvement to the above scheme, the preparation method of the modified kaolin comprises the following steps: calcining kaolin at the temperature of 700-800 ℃ for 2-3 hours at high temperature, stirring the kaolin with 8-12% of sodium hydroxide, 3-5% of sodium bentonite and 6-8% of lignin fiber which are 1.2 times of the kaolin by weight at the temperature of 40-45 ℃ for 3-4 hours, filtering the mixture after the stirring is finished, and drying the aluminum slag at the temperature of 90-95 ℃ to obtain the aluminum-containing composite material.
As a further improvement of the scheme, the volume weight of the composite biochar is 0.1-0.2g/cm for carrying out heavy harvest.
As a further improvement on the above scheme, the content of calcium carbonate in the nano calcium carbonate is > 97%, the specific surface area is 46-50 m/g, and the oil absorption value is < 35Gdop/100gCaCO 3.
As a further improvement of the scheme, the silkworm excrement raw material of the composite biochar is silkworm excrement of three ages or silkworm excrement of four ages.
As a further improvement to the above scheme, the culture substrate has a pH value of 5.7-5.9, an EC of 6.85-8.24mS/cm, a bulk weight of 0.30-0.34g/cm, and is produced by the method with a water holding capacity of 176-80%, a total porosity of 75-80% and a vent porosity of 58-60%.
The heating temperature and the raw material ratio of the composite biochar are strictly limited in the preparation method of the composite biochar, and if the heating temperature is changed, the physicochemical properties of the composite biochar are influenced.
Compared with the prior art, the invention has the following advantages: the composite biochar prepared by the invention has rich pore structures, is matched with other raw materials, increases the porosity of the matrix, reduces the volume weight, reduces the tensile strength, ensures that the matrix has better water retention and fertilizer retention, reduces the loss of nutrient components in the matrix, thereby influencing the root system distribution of the culture matrix, ensures that seeds in the obtained culture matrix easily germinate, and ensures that the root systems of crops can effectively absorb water and mineral elements in the matrix; the composite biochar and the modified kaolin have rich functional groups, have higher CEC, can improve the total charge of the matrix, have an effect of improving the microbial activity in the matrix, and further can improve the fertility of the matrix so as to promote the growth of crops; the nano calcium carbonate and the composite biochar act in a matched manner, so that the absorption of harmful substances after organic matters are rotten can be enhanced, the pH value of a matrix can be increased, the contents of potassium, phosphorus and organic matters can be increased, the cultivation yield of the dendrobium officinale is increased, the accumulation of total polysaccharide components in the dendrobium officinale is increased, and the obtained dendrobium officinale has higher top grade and use value.
Detailed Description
Example 1
A culture medium for improving quality of dendrobium officinale comprises the following raw materials in parts by weight: 22 parts of pepper branch fermentation material, 6 parts of coconut husk, 14 parts of composite biochar, 4 parts of nano calcium carbonate, 2 parts of modified kaolin and 4 parts of diatomite;
the preparation method of the composite biochar comprises the following steps: mixing silkworm excrement, rice straw, reed powder slag and volcanic rock according to a weight ratio of 14:7:3:1.5, putting the mixture into a muffle furnace with a programmed temperature control for sampling and pyrolysis, heating the mixture to 350 ℃ by a program of 5 ℃/min, preserving the temperature for 2 hours, continuing heating the mixture to 600 ℃ by a program of 10 ℃/min after the completion, preserving the temperature for 50 minutes, naturally cooling the mixture to room temperature, taking out the mixture, and grinding the mixture until the particle size is 0.8-2mm to obtain the feed additive;
the preparation method of the pepper branch fermented material comprises the following steps: crushing pepper branches into particles with the particle size of 8-10mm, adding 12% of fine sawdust and 4% of erythrina bark by weight, uniformly mixing, soaking for 3 hours by using an acetic acid solution with the mass concentration of 3%, stacking in a greenhouse, covering by using a plastic film, fermenting for 14 hours at the temperature of 38 ℃, and naturally spreading and airing for 5 hours; and (3) before transplanting, sterilizing the fermented material by using a mixed solution of 50% of carbendazim and 45% of chlorpyrifos to obtain the pepper branch fermented material.
The preparation method of the modified kaolin comprises the following steps: calcining kaolin at 750 ℃ for 2.5 hours at high temperature, stirring the kaolin with 10% sodium hydroxide, 4% sodium bentonite and 7% lignin fiber which are 1.2 times of the kaolin by weight at 42 ℃ for 3.5 hours, filtering the mixture after the stirring is finished, and drying the aluminum slag at 92 ℃ to obtain the aluminum-containing composite material; the volume weight of the composite biochar is 0.1-0.2g/cm for high yield; the content of calcium carbonate in the nano calcium carbonate is more than 97 percent, the specific surface area is 46-50 m/g, and the oil absorption value is less than 35Gdop/100gCaCO 3; the silkworm excrement raw material of the composite biochar is silkworm excrement of three ages.
The culture substrate has a pH value of 5.7-5.9, an EC of 6.85-8.24mS/cm, a bulk density of 0.30-0.34g/cm, a water holding capacity of 176-182%, a total porosity of 75-80% and a vent pore volume of 58-60%.
Example 2
A culture medium for improving quality of dendrobium officinale comprises the following raw materials in parts by weight: 18 parts of pepper branch fermentation material, 8 parts of coconut husk, 12 parts of composite biochar, 5 parts of nano calcium carbonate, 3 parts of modified kaolin and 2 parts of diatomite;
the preparation method of the composite biochar comprises the following steps: mixing silkworm excrement, rice straw, reed powder slag and volcanic rock according to a weight ratio of 16:6:4:1, putting the mixture into a muffle furnace with a program temperature control for sampling pyrolysis, heating the mixture to 350 ℃ with a program of 5 ℃/min, preserving the heat for 2.5 hours, continuing heating the mixture to 600 ℃ with a program of 10 ℃/min after the completion, preserving the heat for 60 minutes, naturally cooling the mixture to room temperature, taking out the mixture, and grinding the mixture until the particle size is 0.8-2mm to obtain the feed additive;
the preparation method of the pepper branch fermented material comprises the following steps: crushing pepper branches into particles with the particle size of 8-10mm, adding fine sawdust accounting for 10% of the weight of the pepper branches and 5% of erythrina bark, uniformly mixing, soaking for 4 hours by using an acetic acid solution with the mass concentration of 2%, stacking in a greenhouse, covering by using a plastic film, fermenting for 16 hours at the temperature of 40 ℃, and naturally spreading and drying for 4 hours; and (3) before transplanting, sterilizing the fermented material by using a mixed solution of 50% of carbendazim and 45% of chlorpyrifos to obtain the pepper branch fermented material.
The preparation method of the modified kaolin comprises the following steps: calcining kaolin at 800 ℃ for 3 hours to complete stirring treatment of 12% sodium hydroxide, 5% sodium bentonite and 6% lignin fiber which are 1.2 times of the kaolin in weight at 45 ℃ for 4 hours, filtering, and drying aluminum slag at 95 ℃ to obtain the aluminum-containing slag; the volume weight of the composite biochar is 0.1-0.2g/cm for high yield; the content of calcium carbonate in the nano calcium carbonate is more than 97 percent, the specific surface area is 46-50 m/g, and the oil absorption value is less than 35Gdop/100gCaCO 3; the silkworm excrement raw material of the composite biochar is silkworm excrement of four ages.
The culture substrate has a pH value of 5.7-5.9, an EC of 6.85-8.24mS/cm, a bulk density of 0.30-0.34g/cm, a water holding capacity of 176-182%, a total porosity of 75-80% and a vent pore volume of 58-60%.
Example 3
A culture medium for improving quality of dendrobium officinale comprises the following raw materials in parts by weight: 26 parts of pepper branch fermentation material, 4 parts of coconut husk, 16 parts of composite biochar, 3 parts of nano calcium carbonate, 1 part of modified kaolin and 6 parts of diatomite;
the preparation method of the composite biochar comprises the following steps: mixing silkworm excrement, rice straw, reed powder slag and volcanic rock in a weight ratio of 12:8:2:2, putting the mixture into a muffle furnace with a program temperature control for sampling pyrolysis, heating to 350 ℃ by a program of 5 ℃/min, preserving heat for 1.5 hours, continuing to heat to 600 ℃ by a program of 10 ℃/min after finishing, preserving heat for 40 minutes, naturally cooling to room temperature, taking out, and grinding until the particle size is 0.8-2 mm;
the preparation method of the pepper branch fermented material comprises the following steps: crushing pepper branches into particles with the particle size of 8-10mm, adding fine sawdust accounting for 14% of the weight of the pepper branches and erythrina bark accounting for 3% of the weight of the pepper branches, uniformly mixing, soaking the mixture for 2 hours by using an acetic acid solution with the mass concentration of 4%, stacking the mixture in a greenhouse, covering the mixture by using a plastic film, fermenting the mixture for 12 hours at the temperature of 35 ℃, and naturally spreading and drying the mixture for 6 hours; and (3) before transplanting, sterilizing the fermented material by using a mixed solution of 50% of carbendazim and 45% of chlorpyrifos to obtain the pepper branch fermented material.
The preparation method of the modified kaolin comprises the following steps: calcining kaolin at a high temperature of 700 ℃ for 2 hours, stirring the kaolin with 8% sodium hydroxide, 6% sodium bentonite and 8% lignin fiber which are 1.2 times of the kaolin in weight at a temperature of 40 ℃ for 3 hours, filtering the mixture after the stirring, and drying the aluminum slag at a temperature of 90 ℃ to obtain the aluminum-containing composite material; the volume weight of the composite biochar is 0.1-0.2g/cm for high yield; the content of calcium carbonate in the nano calcium carbonate is more than 97 percent, the specific surface area is 46-50 m/g, and the oil absorption value is less than 35Gdop/100gCaCO 3; the silkworm excrement raw material of the composite biochar is silkworm excrement of three ages or silkworm excrement of four ages.
The culture substrate has a pH value of 5.7-5.9, an EC of 6.85-8.24mS/cm, a bulk density of 0.30-0.34g/cm, a water holding capacity of 176-182%, a total porosity of 75-80% and a vent pore volume of 58-60%.
Setting a control group 1, replacing the mixture of the pepper branches, the fine sawdust and the erythrina bark in the embodiment 1 with pine bark with equal weight, and keeping the rest contents unchanged; setting a control group 2, removing the reed residue in the preparation process of the composite biochar in the embodiment 1, and keeping the rest contents unchanged; setting a control group 3, heating the composite biochar in the preparation process of the composite biochar in the embodiment 1 in stages to 600 ℃ at a speed of 8 ℃/min, keeping for 2 hours and 50 minutes, and keeping the rest of the contents unchanged; setting a comparison group 4, and removing the modified kaolin in the example 1 to ensure that the rest contents are unchanged;
the method comprises the following steps of taking dendrobium officinale seedlings provided by a local laboratory as an experimental surface, transplanting the dendrobium officinale seedlings in 2016, 4 and 15 days, and detecting the content of water-soluble polysaccharides in the dendrobium officinale after the dendrobium officinale seedlings are transplanted for 280 days (the content of the dendrobium officinale polysaccharides is not less than 25.0% in terms of anhydrous glucose according to the regulation of Chinese pharmacopoeia 2010 edition), so as to obtain the following results:
TABLE 1
As can be seen from the data in the table 1, compared with the prior art, the method provided by the invention is obviously improved, which indicates that the plant has better medicinal value, and meanwhile, the reasonable preparation of the pepper branch fermentation material can effectively replace pine bark and alleviate the problem of insufficient raw material of the pine bark.
Claims (6)
1. A culture medium for improving the quality of dendrobium officinale is characterized by comprising the following raw materials in parts by weight: 18-26 parts of pepper branch fermentation material, 4-8 parts of coconut husk, 12-16 parts of composite biochar, 3-5 parts of nano calcium carbonate, 1-3 parts of modified kaolin and 2-6 parts of diatomite;
the preparation method of the composite biochar comprises the following steps: mixing silkworm excrement, rice straw, reed powder slag and volcanic rock in a weight ratio of 12-16:6-8:2-4:1-2, putting the mixture into a muffle furnace with a program temperature control for sampling pyrolysis, heating to 350 ℃ by a program of 5 ℃/min, preserving heat for 1.5-2.5 hours, continuing heating to 600 ℃ by a program of 10 ℃/min after the completion, preserving heat for 40-60 minutes, naturally cooling to room temperature, taking out, and grinding until the particle size is 0.8-2 mm;
the preparation method of the pepper branch fermented material comprises the following steps: crushing pepper branches into particles with the particle size of 8-10mm, adding fine sawdust accounting for 10-14% of the weight of the pepper branches and erythrina bark accounting for 3-5% of the weight of the pepper branches, uniformly mixing, soaking the mixture for 2-4 hours by using an acetic acid solution with the mass concentration of 2-4%, stacking the mixture in a greenhouse, covering the mixture by using a plastic film, fermenting the mixture for 12-16 hours at the temperature of 35-40 ℃, and naturally spreading and drying the mixture for 4-6 hours; and (3) before transplanting, sterilizing the fermented material by using a mixed solution of 50% of carbendazim and 45% of chlorpyrifos to obtain the pepper branch fermented material.
2. The cultivation substrate for improving the quality of dendrobium officinale of claim 1, wherein the preparation method of the modified kaolin comprises the following steps: calcining kaolin at the temperature of 700-800 ℃ for 2-3 hours at high temperature, stirring the kaolin with 8-12% of sodium hydroxide, 3-5% of sodium bentonite and 6-8% of lignin fiber which are 1.2 times of the kaolin by weight at the temperature of 40-45 ℃ for 3-4 hours, filtering the mixture after the stirring is finished, and drying the aluminum slag at the temperature of 90-95 ℃ to obtain the aluminum-containing composite material.
3. The culture medium for improving the quality of dendrobium officinale according to claim 1, wherein the composite biochar is subjected to thin film planting at a volume weight of 0.1-0.2 g/cm.
4. The culture substrate for improving the quality of dendrobium officinale according to claim 1, wherein the content of calcium carbonate in the nano calcium carbonate is > 97%, the specific surface area is 46-50 m/g, and the oil absorption value is < 35Gdop/100g caco 3.
5. The cultivation substrate for improving the quality of dendrobium officinale according to claim 1, wherein the silkworm excrement raw material of the composite charcoal is three-year-old silkworm excrement or four-year-old silkworm excrement.
6. The cultivation substrate for improving the quality of Dendrobium officinale as claimed in claim 1, wherein the cultivation substrate has a pH of 5.7-5.9, an EC of 6.85-8.24mS/cm, a bulk density of 0.30-0.34g/cm, a water holding capacity of 176-182%, a total porosity of 75-80%, and a ventilation pore of 58-60%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810422777.5A CN108739246B (en) | 2018-05-05 | 2018-05-05 | Culture medium for improving quality of dendrobium officinale |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810422777.5A CN108739246B (en) | 2018-05-05 | 2018-05-05 | Culture medium for improving quality of dendrobium officinale |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108739246A CN108739246A (en) | 2018-11-06 |
| CN108739246B true CN108739246B (en) | 2020-09-29 |
Family
ID=64009193
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810422777.5A Active CN108739246B (en) | 2018-05-05 | 2018-05-05 | Culture medium for improving quality of dendrobium officinale |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108739246B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109348968A (en) * | 2018-12-04 | 2019-02-19 | 李凤燕 | A kind of mulberry tree dwarfing planting method |
| CN109937845A (en) * | 2019-02-22 | 2019-06-28 | 霍山岳衡茧丝绸有限公司 | A kind of production method improving Dendrobidium huoshanness alkaloid matrix |
| CN112136646A (en) * | 2020-09-29 | 2020-12-29 | 磐安县方正珍稀药材开发有限公司 | Dendrobium officinale facility cultivation medium and preparation method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105028159A (en) * | 2015-09-01 | 2015-11-11 | 宣威市农硕农特产品开发有限公司 | Imitation wild planting substrate for dendrobium officinale and preparing method |
| CN105248262A (en) * | 2015-10-08 | 2016-01-20 | 浙江省农业科学院 | Pine bark-biochar matrix for culture of Dendrobium officinale |
| CN105766591A (en) * | 2016-04-25 | 2016-07-20 | 广西智宝科技有限公司 | Dendrobium officinale culture medium and preparation method thereof |
| CN105850684A (en) * | 2016-04-27 | 2016-08-17 | 凌云县长生仙草生物科技开发有限公司 | Dendrobium candidum planting substrate containing modified biochar and preparation method of Dendrobium candidum planting substrate |
| CN106069322A (en) * | 2016-06-17 | 2016-11-09 | 枞阳县白云生态园林有限责任公司 | A kind of high-quality Fructus actinidiae chinensis planting matrix |
| CN107821163A (en) * | 2017-11-07 | 2018-03-23 | 安徽同济生生物科技有限公司 | A kind of stem of noble dendrobium polyploid special culture media and preparation method thereof |
| CN107864823A (en) * | 2017-09-30 | 2018-04-03 | 安徽紫瑞园林有限公司 | A kind of dendrobium candidum planting matrix |
-
2018
- 2018-05-05 CN CN201810422777.5A patent/CN108739246B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105028159A (en) * | 2015-09-01 | 2015-11-11 | 宣威市农硕农特产品开发有限公司 | Imitation wild planting substrate for dendrobium officinale and preparing method |
| CN105248262A (en) * | 2015-10-08 | 2016-01-20 | 浙江省农业科学院 | Pine bark-biochar matrix for culture of Dendrobium officinale |
| CN105766591A (en) * | 2016-04-25 | 2016-07-20 | 广西智宝科技有限公司 | Dendrobium officinale culture medium and preparation method thereof |
| CN105850684A (en) * | 2016-04-27 | 2016-08-17 | 凌云县长生仙草生物科技开发有限公司 | Dendrobium candidum planting substrate containing modified biochar and preparation method of Dendrobium candidum planting substrate |
| CN106069322A (en) * | 2016-06-17 | 2016-11-09 | 枞阳县白云生态园林有限责任公司 | A kind of high-quality Fructus actinidiae chinensis planting matrix |
| CN107864823A (en) * | 2017-09-30 | 2018-04-03 | 安徽紫瑞园林有限公司 | A kind of dendrobium candidum planting matrix |
| CN107821163A (en) * | 2017-11-07 | 2018-03-23 | 安徽同济生生物科技有限公司 | A kind of stem of noble dendrobium polyploid special culture media and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 生物炭替代泥炭栽培基质对铁皮石解生长的影响;陈庆飞 等;《中国农学通报》;20150531;第31卷(第13期);30-35 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108739246A (en) | 2018-11-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102919018B (en) | Cultivation method for high-quality oil-tea large seedlings | |
| CN108739246B (en) | Culture medium for improving quality of dendrobium officinale | |
| CN109400372B (en) | Biochar soil improvement organic fertilizer and preparation method thereof | |
| CN105123330A (en) | Seedling culturing substrate containing Chinese medicine residues and application of seedling culturing substrate in salt golden cypress bonsai seedling culturing | |
| CN106234178B (en) | Special substrate for camellia oleifera seedling culture | |
| CN106416799A (en) | Vegetable culture medium | |
| CN107318616A (en) | A kind of Camless valve train method for culturing seedlings of grape | |
| CN107568012A (en) | A kind of coal mine waste rock area mycorhiza auxiliary ecological restoring method based on lavender | |
| CN107500948A (en) | A kind of soilless culture planting matrix and preparation method thereof | |
| CN109511482B (en) | Compound management method for planting lemongrass in leaf moringa oleifera forest | |
| CN109328959B (en) | Method for cultivating new variety of rhododendron | |
| CN111615993B (en) | Preparation and maintenance method of mushroom sticks of short-age mushrooms sowed in winter and spring | |
| KR101905676B1 (en) | Organic liquid fertilizer for Orchidaceae and manufacturing method thereof | |
| CN105052703A (en) | Neofinetia falcata cultivating method | |
| CN110946054A (en) | Tea tree culture medium and preparation method thereof | |
| CN104285654A (en) | High-quality flue-cured tobacco root cultivation method | |
| CN114904900A (en) | Method for promoting sweet sorghum to repair heavy metal contaminated soil by using fungi and biochar | |
| CN110012769B (en) | Teak mycorrhizal light-matrix container seedling raising method | |
| CN107739222A (en) | A kind of strawberry organic soilless cultivation composite interstitial substance | |
| CN114149808A (en) | Soil conditioner for preventing and treating tobacco bacterial wilt and improving proportion of middle-grade and high-grade tobacco leaves and preparation method thereof | |
| CN105766282A (en) | Method for gastrodia elata sexual propagation through bamboo wood | |
| Tokman | Optimization of elements of cultivation technology of ornamentals in the Nort-eastery part of forest Steppe of Ukraine | |
| CZ305666B6 (en) | Process for preparing biochar-based formulation for plant growth support | |
| CN108467297A (en) | A kind of passion fruit fertilizer | |
| CN111226745A (en) | Bed soil composition easy to root and used for flowers and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20231107 Address after: Taiping fan Xiang Wang Jia Dian Cun, Huoshan County, Lu'an City, Anhui Province Patentee after: ANHUI HUOSHAN NONGBOLE DEVELOPMENT CO.,LTD. Address before: Taiping fan Xiang Wang Jia Dian Cun, Huoshan County, Lu'an City, Anhui Province Patentee before: ANHUI HUOSHAN HUHUALANG DENDROBE DEVELOPMENT Co.,Ltd. |