CN107821163A - A kind of stem of noble dendrobium polyploid special culture media and preparation method thereof - Google Patents
A kind of stem of noble dendrobium polyploid special culture media and preparation method thereof Download PDFInfo
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- CN107821163A CN107821163A CN201711085794.6A CN201711085794A CN107821163A CN 107821163 A CN107821163 A CN 107821163A CN 201711085794 A CN201711085794 A CN 201711085794A CN 107821163 A CN107821163 A CN 107821163A
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- stem
- noble dendrobium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Abstract
The invention discloses a kind of stem of noble dendrobium polyploid special culture media and preparation method thereof, it is related to culture medium technical field.Stem of noble dendrobium polyploid special culture media of the present invention, include the component of following parts by weight：100~160 parts of bog moss, 40~60 parts of mud, 15~30 parts of modified kaolin, 16~30 parts of vegetable material, 10~22 parts of EDETATE SODIUM, 3~8 parts of ferric sulfate, 3~8 parts of ammonium sulfate, 5~12 parts of potassium sulfate, 3~6 parts of epsom salt, 2~5 parts of anhydrous potassium dihydrogenphosphate, 3~7 parts of glucose, 5~10 parts of peptone, 6~16 parts of Tomato juice, 3~8 parts of rose wate, 4~8 parts of agar, 8~15 parts of activated carbon, 20~36 parts of water；Its preparation method includes conventional mixing, crushing, drying.Nutrition arrangement of the present invention is balanced, meets the growth demand of stem of noble dendrobium polyploid so that the growth coefficient of stem of noble dendrobium polyploid is high, the speed of growth is fast, transplanting survival rate is high.
Description
Technical field：
The present invention relates to culture medium technical field, and in particular to a kind of stem of noble dendrobium polyploid special culture media and its preparation side
Method.
Background technology：
Contain the compounds such as dendrobium polysaccharide, alkaloid, chrysotoxene and EN, and 7 kinds of amino needed by human in the stem of noble dendrobium
Acid and various trace elements, there is the functions such as antitumor, anti-aging, strengthen immunity, be to angiocarpy, digestive system, breathing
System and ophthalmology disease have obvious therapeutic action.Stem of noble dendrobium polyploid is mainly used under isolated culture condition at present, and inductor is thin
The mode of born of the same parents' chromosome doubling obtains.By genome tripling, can obtain with polyploid and cenospecies double dominant
Triploid, improve root yield, quality and the resistance of the stem of noble dendrobium.
In recent years, the artificial culturing method of stem of noble dendrobium polyploid is due to no special culture medium, however it remains seeding propagation
The problems such as speed is slow, training tissue culture seedling and transplanting survival rate are low, seriously constrain industrialization, the scale hair of stem of noble dendrobium polyploid
Exhibition.
The content of the invention：
The technical problems to be solved by the invention are to provide a kind of stem of noble dendrobium polyploid special culture media and preparation method thereof,
The culture medium is nutritious, prepares simple, the plant hormone without chemical composition so that the growth coefficient height of stem of noble dendrobium polyploid,
The speed of growth is fast, transplanting survival rate is high.
The technical problems to be solved by the invention are realized using following technical scheme：
A kind of stem of noble dendrobium polyploid special culture media, include the component of following parts by weight：100~160 parts of bog moss, mud 40
~60 parts, 15~30 parts of modified kaolin, 16~30 parts of vegetable material, 10~22 parts of EDETATE SODIUM, 3~8 parts of ferric sulfate, sulfuric acid
3~8 parts of ammonium, 5~12 parts of potassium sulfate, 3~6 parts of epsom salt, 2~5 parts of anhydrous potassium dihydrogenphosphate, 3~7 parts of glucose, egg
White 5~10 parts of peptone, 6~16 parts of Tomato juice, 3~8 parts of rose wate, 4~8 parts of agar, 8~15 parts of activated carbon, water 20~36
Part.
Preferably, the mud is sludge of sewage treatment plant, water eutrophication river bottom mud, water eutrophication bottom mud in lake
One or more combinations in bed mud.
Preferably, the vegetable material is one or more combinations in agricultural crop straw, pine needle, dead twigs and withered leaves.
Preferably, the modified kaolin is 5 that 250~400 parts by weight are soaked in by the kaolin of 20~30 parts by weight
In~8wt% hydrochloric acid solution, after centrifuging 5~10min, filtration drying obtains.
The preparation method of above-mentioned stem of noble dendrobium polyploid special culture media, comprises the following steps：
1) bog moss, modified kaolin, vegetable material are mixed, dries to water content and be less than 5%, be ground into particle diameter and be less than
3mm hybrid particles；
2) hybrid particles, mud are sufficiently mixed uniformly, 120~150 DEG C are dried to porosity and reach 50~80%, nature
It is standby to be down to room temperature；
3) by the mixture of step 2) and EDETATE SODIUM, ferric sulfate, ammonium sulfate, potassium sulfate, epsom salt, anhydrous phosphoric acid
Potassium dihydrogen, glucose, peptone, Tomato juice, rose wate, agar, activated carbon, water are mixed evenly, after ultraviolet sterilization
Obtain the special culture media.
Preferably, mixture made from the step 2) is crushed to 1~3mm of particle diameter.
The beneficial effects of the invention are as follows：1st, stem of noble dendrobium polyploid special culture media of the invention, with rich in trace element, mineral
The bog moss of matter and water storage function admirable is main component, coordinates modified kaolin, vegetable material to be breathed freely more as matrix body
Hole, it is not easy hardened；Coordinate other a variety of N P and K salt, buffer, carbohydrate, protein-based additives, nutrition arrangement is balanced, symbol
Close the growth demand of stem of noble dendrobium polyploid, the plant hormone without chemical composition so that the growth coefficient of stem of noble dendrobium polyploid is high, growth
Speed is fast, transplanting survival rate is high.
2nd, the activated carbon in culture medium of the invention has good absorption property, huge surface area and complicated hole
Structure, colourity, stink, toxic metals can be removed, green matrix environment is provided for the growth of stem of noble dendrobium polyploid；Rose
Rare colored water contains the active ingredients such as multiple proteins, tannin, cyclic peptide, is advantageous to improve bactericidal performance, the activity of culture medium.
Embodiment：
In order that the technical means, the inventive features, the objects and the advantages of the present invention are easy to understand, tie below
Specific embodiment is closed, the present invention is expanded on further.
Embodiment 1
A kind of stem of noble dendrobium polyploid special culture media, include the component of following parts by weight：135 parts of bog moss, sewage treatment plant
45 parts of sludge, 23 parts of modified kaolin, 22 parts of agricultural crop straw, 15 parts of EDETATE SODIUM, 6 parts of ferric sulfate, 5 parts of ammonium sulfate, sulfuric acid
7 parts of potassium, 4 parts of epsom salt, 3 parts of anhydrous potassium dihydrogenphosphate, 5 parts of glucose, 8 parts of peptone, 11 parts of Tomato juice, rose
6 parts of water, 5 parts of agar, 13 parts of activated carbon, 25 parts of water.Wherein, modified kaolin is to be soaked in 286 by the kaolin of 23 parts by weight
In the 6wt% of parts by weight hydrochloric acid solution, after centrifuging 8min, filtration drying obtains.
The preparation method of above-mentioned stem of noble dendrobium polyploid special culture media, comprises the following steps：
1) bog moss, modified kaolin, vegetable material are mixed, dries to water content and be less than 5%, be ground into particle diameter and be less than
3mm hybrid particles；
2) hybrid particles, mud are sufficiently mixed uniformly, 136 DEG C are dried to porosity and reach 65%, are down to room temperature naturally,
It is standby to be crushed to 1~3mm of particle diameter；
3) by the mixture of step 2) and EDETATE SODIUM, ferric sulfate, ammonium sulfate, potassium sulfate, epsom salt, anhydrous phosphoric acid
Potassium dihydrogen, glucose, peptone, Tomato juice, rose wate, agar, activated carbon, water are mixed evenly, after ultraviolet sterilization
Obtain the special culture media.
Special culture media prepared by the embodiment is 99.2% to the seedling percent of stem of noble dendrobium polyploid, and transplanting survival rate is
99.5%, pest and disease damage rate is 1.6%.
Embodiment 2
A kind of stem of noble dendrobium polyploid special culture media, include the component of following parts by weight：145 parts of bog moss, water eutrophication
55 parts of river bottom mud, 24 parts of modified kaolin, 20 parts of pine needle, 18 parts of EDETATE SODIUM, 7 parts of ferric sulfate, 5 parts of ammonium sulfate, potassium sulfate
10 parts, 6 parts of epsom salt, 5 parts of anhydrous potassium dihydrogenphosphate, 7 parts of glucose, 8 parts of peptone, 15 parts of Tomato juice, rose
7 parts of water, 5 parts of agar, 10 parts of activated carbon, 33 parts of water.Wherein, modified kaolin is to be soaked in 360 by the kaolin of 30 parts by weight
In the 7wt% of parts by weight hydrochloric acid solution, after centrifuging 10min, filtration drying obtains.
The preparation method of above-mentioned stem of noble dendrobium polyploid special culture media, comprises the following steps：
1) bog moss, modified kaolin, vegetable material are mixed, dries to water content and be less than 5%, be ground into particle diameter and be less than
3mm hybrid particles；
2) hybrid particles, mud are sufficiently mixed uniformly, 140 DEG C are dried to porosity and reach 70%, are down to room temperature naturally,
It is standby to be crushed to 1~3mm of particle diameter；
3) by the mixture of step 2) and EDETATE SODIUM, ferric sulfate, ammonium sulfate, potassium sulfate, epsom salt, anhydrous phosphoric acid
Potassium dihydrogen, glucose, peptone, Tomato juice, rose wate, agar, activated carbon, water are mixed evenly, after ultraviolet sterilization
Obtain the special culture media.
Special culture media prepared by the embodiment is 98.8% to the seedling percent of stem of noble dendrobium polyploid, and transplanting survival rate is
99.4%, pest and disease damage rate is 1.9%.
Embodiment 3
A kind of stem of noble dendrobium polyploid special culture media, include the component of following parts by weight：160 parts of bog moss, water eutrophication
60 parts of bottom mud in lake, 30 parts of modified kaolin, 23 parts of dead twigs and withered leaves, 18 parts of EDETATE SODIUM, 8 parts of ferric sulfate, 7 parts of ammonium sulfate, sulphur
Sour 12 parts of potassium, 6 parts of epsom salt, 5 parts of anhydrous potassium dihydrogenphosphate, 7 parts of glucose, 9 parts of peptone, 15 parts of Tomato juice, rose
Rare 8 parts of colored water, 7 parts of agar, 12 parts of activated carbon, 36 parts of water.Wherein, modified kaolin is soaked in by the kaolin of 30 parts by weight
In the 5wt% of 400 parts by weight hydrochloric acid solution, after centrifuging 10min, filtration drying obtains.
The preparation method of above-mentioned stem of noble dendrobium polyploid special culture media, comprises the following steps：
1) bog moss, modified kaolin, vegetable material are mixed, dries to water content and be less than 5%, be ground into particle diameter and be less than
3mm hybrid particles；
2) hybrid particles, mud are sufficiently mixed uniformly, 150 DEG C are dried to porosity and reach 80%, are down to room temperature naturally,
It is standby to be crushed to 1~3mm of particle diameter；
3) by the mixture of step 2) and EDETATE SODIUM, ferric sulfate, ammonium sulfate, potassium sulfate, epsom salt, anhydrous phosphoric acid
Potassium dihydrogen, glucose, peptone, Tomato juice, rose wate, agar, activated carbon, water are mixed evenly, after ultraviolet sterilization
Obtain the special culture media.
Special culture media prepared by the embodiment is 98.2% to the seedling percent of stem of noble dendrobium polyploid, and transplanting survival rate is
99.1%, pest and disease damage rate is 2.4%.
Comparative example
A kind of stem of noble dendrobium polyploid special culture media, include the component of following parts by weight：135 parts of bog moss, sewage treatment plant
45 parts of sludge, 22 parts of agricultural crop straw, 15 parts of EDETATE SODIUM, 6 parts of ferric sulfate, 5 parts of ammonium sulfate, 7 parts of potassium sulfate, epsom salt
4 parts, it is 3 parts of anhydrous potassium dihydrogenphosphate, 5 parts of glucose, 8 parts of peptone, 11 parts of Tomato juice, 6 parts of rose wate, 5 parts of agar, living
13 parts of charcoal of property, 25 parts of water.
The preparation method of above-mentioned stem of noble dendrobium polyploid special culture media, comprises the following steps：
1) bog moss, vegetable material are mixed, dries to water content and be less than 5%, be ground into the mixing that particle diameter is less than 3mm
Grain；
2) hybrid particles, mud are sufficiently mixed uniformly, 136 DEG C are dried to porosity and reach 65%, are down to room temperature naturally,
It is standby to be crushed to 1~3mm of particle diameter；
3) by the mixture of step 2) and EDETATE SODIUM, ferric sulfate, ammonium sulfate, potassium sulfate, epsom salt, anhydrous phosphoric acid
Potassium dihydrogen, glucose, peptone, Tomato juice, rose wate, agar, activated carbon, water are mixed evenly, after ultraviolet sterilization
Obtain the special culture media.
Special culture media prepared by the comparative example is 83.3% to the seedling percent of stem of noble dendrobium polyploid, and transplanting survival rate is
91.4%, pest and disease damage rate is 5.4%.
The general principle and principal character and advantages of the present invention of the present invention has been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the simply explanation described in above-described embodiment and specification is originally
The principle of invention, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (6)
1. a kind of stem of noble dendrobium polyploid special culture media, it is characterised in that include the component of following parts by weight：Bog moss 100~160
Part, 40~60 parts of mud, 15~30 parts of modified kaolin, 16~30 parts of vegetable material, 10~22 parts of EDETATE SODIUM, ferric sulfate 3
~8 parts, 3~8 parts of ammonium sulfate, 5~12 parts of potassium sulfate, 3~6 parts of epsom salt, 2~5 parts of anhydrous potassium dihydrogenphosphate, glucose
3~7 parts, 5~10 parts of peptone, 6~16 parts of Tomato juice, 3~8 parts of rose wate, 4~8 parts of agar, 8~15 parts of activated carbon,
20~36 parts of water.
2. stem of noble dendrobium polyploid special culture media according to claim 1, it is characterised in that the mud is sewage treatment plant
One or more combinations in sludge, water eutrophication river bottom mud, water eutrophication bottom mud in lake.
3. stem of noble dendrobium polyploid special culture media according to claim 1, it is characterised in that the vegetable material is crops
One or more combinations in stalk, pine needle, dead twigs and withered leaves.
4. stem of noble dendrobium polyploid special culture media according to claim 1, it is characterised in that the modified kaolin is by 20
The kaolin of~30 parts by weight is soaked in 5~8wt% of 250~400 parts by weight hydrochloric acid solution, centrifuges 5~10min
Afterwards, filtration drying obtains.
5. the preparation method of the stem of noble dendrobium polyploid special culture media according to any one of Claims 1 to 4, it is characterised in that
Comprise the following steps：
1) bog moss, modified kaolin, vegetable material are mixed, dries to water content and be less than 5%, be ground into particle diameter and be less than 3mm
Hybrid particles；
2) hybrid particles, mud are sufficiently mixed uniformly, 120~150 DEG C are dried to porosity and reach 50~80%, are down to naturally
Room temperature is standby；
3) by the mixture of step 2) and EDETATE SODIUM, ferric sulfate, ammonium sulfate, potassium sulfate, epsom salt, anhydrous phosphoric acid dihydro
Potassium, glucose, peptone, Tomato juice, rose wate, agar, activated carbon, water are mixed evenly, and are obtained after ultraviolet sterilization
The special culture media.
6. the preparation method of stem of noble dendrobium polyploid special culture media according to claim 5, it is characterised in that the step 2)
Obtained mixture is crushed to 1~3mm of particle diameter.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108739246A (en) * | 2018-05-05 | 2018-11-06 | 安徽霍山斛花郎石斛开发有限公司 | A kind of cultivation matrix improving dendrobium candidum quality |
| CN109439608A (en) * | 2018-11-16 | 2019-03-08 | 安徽骏旌环保科技有限公司 | A kind of duck class excrement decomposition culture medium |
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| CN107155587A (en) * | 2017-05-27 | 2017-09-15 | 霍山宝信园石斛开发有限公司 | A kind of implantation methods of the stem of noble dendrobium |
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| CN101766113A (en) * | 2010-01-25 | 2010-07-07 | 大连普瑞康生物技术有限公司 | Method for preparing dendrobium officinale culture medium |
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| CN108739246A (en) * | 2018-05-05 | 2018-11-06 | 安徽霍山斛花郎石斛开发有限公司 | A kind of cultivation matrix improving dendrobium candidum quality |
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