CN109439608A - A kind of duck class excrement decomposition culture medium - Google Patents

A kind of duck class excrement decomposition culture medium Download PDF

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Publication number
CN109439608A
CN109439608A CN201811366246.5A CN201811366246A CN109439608A CN 109439608 A CN109439608 A CN 109439608A CN 201811366246 A CN201811366246 A CN 201811366246A CN 109439608 A CN109439608 A CN 109439608A
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parts
culture medium
decomposes
duck class
bacillus
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CN201811366246.5A
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蒋忠辉
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Anhui Junzhi Environmental Protection Technology Co Ltd
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Anhui Junzhi Environmental Protection Technology Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F11/00—Treatment of sludge; Devices therefor
    • C02F11/02—Biological treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Molecular Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Treatment Of Sludge (AREA)

Abstract

The invention discloses a kind of duck class excrement to decompose culture medium, and raw material includes: 12-16 parts of complex micro organism fungicides, 15-25 parts of wheatfeeds, 10-22 parts of sawdust powders, 8-12 parts of fish meal, 3-7 portions of maltose, 4-8 parts of modified kaolins, 1-2 parts of calcium chloride, 3-5 parts of amino acid, 1-2 parts of dipotassium hydrogen phosphates, 0.3-0.7 parts of dibasic ammonium citrates, 0.5-0.9 parts of ferrous sulfate, 0.5-1.0 parts of gelling agents by weight.Duck class excrement of the invention, which decomposes culture medium, has the function of stronger degradation excrement and deodorization, and the innoxious and recycling treatment of excrement may be implemented, and reduces the pollution to environment.

Description

A kind of duck class excrement decomposition culture medium
Technical field
The present invention relates to microorganisms technical fields, and in particular to a kind of duck class excrement decomposition culture medium.
Background technique
With the scale of livestock and poultry farm, intensive rapid development, the concentration of feces of livestock and poultry and sewage is discharged to ring Border brings huge pollution, feces of livestock and poultry and sewage also contaminated soil, water source, air during stacking, cause around Environment severe exacerbation.Under the major premise of national advocating environment protection, feces of livestock and poultry and sewage and sewage treatment have been urgently to be resolved Environmental problem.
Summary of the invention
Technical problems based on background technology, the invention proposes a kind of duck class excrement decompose culture medium, have compared with The effect of strong degradation excrement and deodorization, realizes the innoxious and recycling treatment of excrement.
The invention proposes a kind of duck class excrement to decompose culture medium, and raw material includes: 12-16 parts of composite microbials by weight Object microbial inoculum, 15-25 part wheatfeed, 10-22 parts of sawdust powders, 8-12 parts of fish meal, 3-7 portions of maltose, 4-8 parts of modified kaolins, 1-2 Part calcium chloride, 3-5 parts of amino acid, 1-2 parts of dipotassium hydrogen phosphates, 0.3-0.7 parts of dibasic ammonium citrates, 0.5-0.9 parts of ferrous sulfate, 0.5-1.0 parts of gelling agents.
Preferably, the complex micro organism fungicide includes bacillus, actinomyces, saccharomycete, Pediococcus acidilactici, thermophilic chain Coccus.
Preferably, the bacillus, actinomyces, saccharomycete, Pediococcus acidilactici, streptococcus thermophilus weight ratio be 7- 11:2-4:8-13:3-5:4-6.
Preferably, the bacillus in bacillus subtilis, bacillus megaterium, bacillus licheniformis at least It is a kind of.
Preferably, the preparation process of the modified kaolin is as follows: kaolin being mixed with water, ultrasonic disperse, room temperature stirs It mixes, stearyl dimethyl benzyl ammonium chloride and silane coupling agent, ultrasonic reaction is added, heating stirring filters, and washs, and it is dry, Grinding, sieving, can be obtained modified kaolin.
Preferably, the kaolin, stearyl dimethyl benzyl ammonium chloride, silane coupling agent weight ratio be 35-38: 1-2:3-5.
Preferably, the temperature of the ultrasonic disperse is 50-60 DEG C, and the time of ultrasonic disperse is 1-2h；Preferably, described normal The time of temperature stirring is 6-10h.
Preferably, the temperature of the ultrasonic reaction is 60-65 DEG C, and the time of ultrasonic reaction is 40-60min；Preferably, institute The temperature for stating heating stirring is 80-85 DEG C, mixing time 2-4h.
Preferably, the temperature of the drying is 100-110 DEG C, drying time 5-8h.
Preferably, the mesh number of the sieving is 200-220 mesh.
Duck class excrement of the present invention decomposes culture medium, has the function of stronger degradation excrement and deodorization, realizes excrement Innoxious and recycling treatment；The present invention is added to wheatfeed, sawdust powder, fish meal, maltose, calcium chloride, amino acid, phosphoric acid hydrogen Dipotassium, dibasic ammonium citrate, ferrous sulfate, enable complex micro organism fungicide quickly to be grown, to realize in excrement The degradation of organic matter；Being added to complex micro organism fungicide includes bacillus, actinomyces, saccharomycete, Pediococcus acidilactici, thermophilic chain The microorganisms such as coccus can effectively decompose the organic substance in duck class excrement and inhibit the pathogenic microorganism in excrement, be divided The growth that energy promotes microorganism as nutriment is converted into after solution；The modified kaolin of addition, can be improved composite microbial The growth rate of object microbial inoculum is conducive to improve the present invention to the degradation rate of excrement organic substance, can also pass through absorption excrement In foul gas reduce the air discharge of odorant, reduce pollution to environment.
Specific embodiment
In the following, technical solution of the present invention is described in detail by specific embodiment.
Embodiment 1
A kind of duck class excrement decomposition culture medium, raw material includes: 16 parts of complex micro organism fungicides, 15 parts of wheat brans by weight Powder, 22 parts of sawdust powders, 8 parts of fish meal, 7 portions of maltose, 4 parts of modified kaolins, 2 parts of calcium chloride, 3 parts of amino acid, 2 parts of phosphoric acid hydrogen two Potassium, 0.3 part of dibasic ammonium citrate, 0.9 part of ferrous sulfate, 0.5 part of gelling agent；
Wherein, the complex micro organism fungicide includes bacillus, actinomyces, saccharomycete, Pediococcus acidilactici, thermophilus Bacterium；The bacillus, actinomyces, saccharomycete, Pediococcus acidilactici, streptococcus thermophilus weight ratio be 11:2:13:3:6；It is described Bacillus selects bacillus subtilis；
The preparation process of the modified kaolin is as follows: kaolin being mixed with water, the ultrasonic disperse 2h at 50 DEG C, then 6h is stirred at normal temperature, and stearyl dimethyl benzyl ammonium chloride and silane coupling agent, the ultrasonic reaction at 65 DEG C is added 40min is then heated at 85 DEG C and stirs 2h, filters, washing, and as 5h dry at 110 DEG C, 220 meshes are crossed in grinding Obtain modified kaolin；
The kaolin, stearyl dimethyl benzyl ammonium chloride, silane coupling agent weight ratio be 35:2:3.
Embodiment 2
A kind of duck class excrement decomposition culture medium, raw material includes: 12 parts of complex micro organism fungicides, 25 parts of wheat brans by weight Powder, 10 parts of sawdust powders, 12 parts of fish meal, 3 portions of maltose, 8 parts of modified kaolins, 1 part of calcium chloride, 5 parts of amino acid, 1 part of phosphoric acid hydrogen Dipotassium, 0.7 part of dibasic ammonium citrate, 0.5 part of ferrous sulfate, 1.0 parts of gelling agents；
Wherein, the complex micro organism fungicide includes bacillus, actinomyces, saccharomycete, Pediococcus acidilactici, thermophilus Bacterium；The bacillus, actinomyces, saccharomycete, Pediococcus acidilactici, streptococcus thermophilus weight ratio be 7:4:8:5:4；The bud Spore bacillus selects bacillus megaterium；
The preparation process of the modified kaolin is as follows: kaolin being mixed with water, the ultrasonic disperse 1h at 60 DEG C, then 10h is stirred at normal temperature, and stearyl dimethyl benzyl ammonium chloride and silane coupling agent, the ultrasonic reaction at 60 DEG C is added 60min is then heated at 80 DEG C and stirs 4h, filters, washing, and as 8h dry at 100 DEG C, 200 meshes are crossed in grinding Obtain modified kaolin；
The kaolin, stearyl dimethyl benzyl ammonium chloride, silane coupling agent weight ratio be 38:1:5.
Embodiment 3
A kind of duck class excrement decomposition culture medium, raw material includes: 14 parts of complex micro organism fungicides, 20 parts of wheat brans by weight Powder, 16 parts of sawdust powders, 10 parts of fish meal, 5 portions of maltose, 6 parts of modified kaolins, 1.5 parts of calcium chloride, 4 parts of amino acid, 2 parts of phosphoric acid Hydrogen dipotassium, 0.5 part of dibasic ammonium citrate, 0.7 part of ferrous sulfate, 0.8 part of gelling agent；
Wherein, the complex micro organism fungicide includes bacillus, actinomyces, saccharomycete, Pediococcus acidilactici, thermophilus Bacterium；The bacillus, actinomyces, saccharomycete, Pediococcus acidilactici, streptococcus thermophilus weight ratio be 9:3:11:4:5；It is described Bacillus selects bacillus subtilis and bacillus licheniformis；
The preparation process of the modified kaolin is as follows: kaolin being mixed with water, the ultrasonic disperse 1.5h at 55 DEG C, so It stirs 8h at normal temperature afterwards, stearyl dimethyl benzyl ammonium chloride and silane coupling agent, the ultrasonic reaction at 62 DEG C is added 50min is then heated at 82 DEG C and stirs 3h, filters, washing, and as 6h dry at 105 DEG C, 200 meshes are crossed in grinding Obtain modified kaolin；
The kaolin, stearyl dimethyl benzyl ammonium chloride, silane coupling agent weight ratio be 36:1.5:4.
Test example 1
The duck class excrement being respectively adopted in 1-3 of the embodiment of the present invention decomposes culture medium and duck class liquid manure by weight 1:300 Mixing carries out fermentation test, and test result is as shown in table 1.
Table 1
Project Organic matter decomposition rate (%) Deodorizing rate (%)
Embodiment 1 94 92
Embodiment 2 95 94
Embodiment 3 96 95
As can be seen from Table 1, duck class excrement of the invention decomposes culture medium, to duck class excrement decomposition excrement with higher Efficiency and deodorizing effect, realize innoxious, the recycling treatment of excrement, do not pollute the environment.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto, Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.

Claims (10)

1. a kind of duck class excrement decomposes culture medium, which is characterized in that its raw material includes: 12-16 parts of complex microorganisms by weight Microbial inoculum, 15-25 part wheatfeed, 10-22 parts of sawdust powders, 8-12 parts of fish meal, 3-7 portions of maltose, 4-8 parts of modified kaolins, 1-2 parts Calcium chloride, 3-5 part amino acid, 1-2 parts of dipotassium hydrogen phosphates, 0.3-0.7 parts of dibasic ammonium citrates, 0.5-0.9 parts of ferrous sulfate, 0.5- 1.0 parts of gelling agents.
2. duck class excrement according to claim 1 decomposes culture medium, which is characterized in that the complex micro organism fungicide includes Bacillus, actinomyces, saccharomycete, Pediococcus acidilactici, streptococcus thermophilus.
3. duck class excrement according to claim 2 decomposes culture medium, which is characterized in that the bacillus, actinomyces, ferment Female bacterium, Pediococcus acidilactici, streptococcus thermophilus weight ratio be 7-11:2-4:8-13:3-5:4-6.
4. duck class excrement according to claim 2 or 3 decomposes culture medium, which is characterized in that the bacillus is selected from withered At least one of careless bacillus, bacillus megaterium, bacillus licheniformis.
5. duck class excrement according to claim 2 decomposes culture medium, which is characterized in that the preparation work of the modified kaolin Skill is as follows: kaolin being mixed, ultrasonic disperse, stirring at normal temperature with water, stearyl dimethyl benzyl ammonium chloride and silane is added Coupling agent, ultrasonic reaction, heating stirring filter, and wash, dry, grind, and modified kaolin can be obtained in sieving.
6. duck class excrement according to claim 5 decomposes culture medium, which is characterized in that the kaolin, octadecyl two Methylbenzyl ammonium chloride, silane coupling agent weight ratio be 35-38:1-2:3-5.
7. duck class excrement according to claim 5 decomposes culture medium, which is characterized in that the temperature of the ultrasonic disperse is 50-60 DEG C, the time of ultrasonic disperse is 1-2h；Preferably, the time of the stirring at normal temperature is 6-10h.
8. duck class excrement according to claim 5 decomposes culture medium, which is characterized in that the temperature of the ultrasonic reaction is 60-65 DEG C, the time of ultrasonic reaction is 40-60min；Preferably, the temperature of the heating stirring is 80-85 DEG C, mixing time For 2-4h.
9. duck class excrement according to claim 5 decomposes culture medium, which is characterized in that the temperature of the drying is 100- 110 DEG C, drying time 5-8h.
10. duck class excrement according to claim 5 decomposes culture medium, which is characterized in that the mesh number of the sieving is 200- 220 mesh.
CN201811366246.5A 2018-11-16 2018-11-16 A kind of duck class excrement decomposition culture medium Pending CN109439608A (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3433123B2 (en) * 1998-11-18 2003-08-04 日清製粉株式会社 Fermentation promoter
CN101591624A (en) * 2009-05-08 2009-12-02 上海华杰生态环境工程有限公司 A kind of preparation method who handles the effectively compound microbial inoculant of human and animal excreta
CN105419317A (en) * 2015-12-25 2016-03-23 南宁学院 Novel nylon 6-polybutylene terephthalate flame retardant alloy material and preparation method thereof
CN106902369A (en) * 2017-02-20 2017-06-30 成都盖尔盖司生物科技有限公司 A kind of microbial treatment method of organic fertilizer raw material
CN107266203A (en) * 2017-07-25 2017-10-20 合肥仙之峰农业科技有限公司 It is a kind of for vegetable cultivation matrix of balcony and preparation method thereof
CN107821163A (en) * 2017-11-07 2018-03-23 安徽同济生生物科技有限公司 A kind of stem of noble dendrobium polyploid special culture media and preparation method thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3433123B2 (en) * 1998-11-18 2003-08-04 日清製粉株式会社 Fermentation promoter
CN101591624A (en) * 2009-05-08 2009-12-02 上海华杰生态环境工程有限公司 A kind of preparation method who handles the effectively compound microbial inoculant of human and animal excreta
CN105419317A (en) * 2015-12-25 2016-03-23 南宁学院 Novel nylon 6-polybutylene terephthalate flame retardant alloy material and preparation method thereof
CN106902369A (en) * 2017-02-20 2017-06-30 成都盖尔盖司生物科技有限公司 A kind of microbial treatment method of organic fertilizer raw material
CN107266203A (en) * 2017-07-25 2017-10-20 合肥仙之峰农业科技有限公司 It is a kind of for vegetable cultivation matrix of balcony and preparation method thereof
CN107821163A (en) * 2017-11-07 2018-03-23 安徽同济生生物科技有限公司 A kind of stem of noble dendrobium polyploid special culture media and preparation method thereof

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Application publication date: 20190308