CN110915581A

CN110915581A - Planting method of rice rich in selenium and SOD (superoxide dismutase)

Info

Publication number: CN110915581A
Application number: CN201911180877.2A
Authority: CN
Inventors: 向世海; 熊云霞
Original assignee: Zhangjiajie Yuquan Ecological Agricultural Development Co Ltd
Current assignee: Zhangjiajie Yuquan Ecological Agricultural Development Co Ltd
Priority date: 2019-11-27
Filing date: 2019-11-27
Publication date: 2020-03-27

Abstract

The invention belongs to the field of agricultural science and technology, and particularly discloses a planting method of selenium-rich and SOD-rich rice. The method comprises the steps of seed treatment, seedling raising, seedling transplanting, fertilization, field management, pest control and harvesting, wherein the steps of: applying an enhancer in the rice planting process, wherein the enhancer comprises bacillus cereus capable of producing SOD and selenium-containing fertilizer. The invention can simultaneously improve the contents of SOD and trace element selenium in rice grains, and the method is simple and convenient.

Description

Planting method of rice rich in selenium and SOD (superoxide dismutase)

Technical Field

The invention relates to the technical field of rice planting.

Background

Selenium (Se) is one of fifteen essential nutrient elements for people every day. According to the medical test, the selenium has the functions of resisting oxidation, resisting aging, preventing and curing diseases, resisting and preventing cancers and neutralizing the toxicity of heavy metals, and the immunologic function of a human body can be reduced due to the selenium deficiency, so that more than 40 diseases are caused. Therefore, selenium is praised by scientists as a 'magic element of life', 'element dominating life' and 'king of human anti-cancer'. More than 40 countries and regions in the world are lack of selenium, and more than 70% of China is lack of selenium, so the selenium supplement work is concerned widely by countries in the world. The dietetic invigoration is superior to the medicinal invigoration, and the selenium-rich rice has the effects of building body and prolonging life after being eaten for a long time. Therefore, the development of selenium-rich rice is a need for the development of functional agriculture.

Free radicals (also called free radicals) are related to most diseases and human aging, and superoxide dismutase (SOD for short) is a natural enemy of oxygen free radicals and is the first killer of the oxygen free radicals in organisms. Due to modern living pressure, environmental pollution, various radiation and excessive motion, a great amount of oxygen radicals are formed; therefore, the position of SOD in biological antioxidants is becoming more and more important.

The rice is called as 'Asian food', and is also the staple food for people in south China. Many of the prior documents disclose methods for increasing the selenium or SOD content, but the methods are only one of 2, and many of the prior documents achieve the effects by applying special fertilizers in the prior rice cultivation process, so that the fertilizer preparation is complicated and the application frequency is high. The Bacillus cereus producing SOD is an endophytic symbiotic bacterium, has more researches in China university of agriculture, can be popularized in fruit trees, can greatly improve the SOD yield, has expired in the bacterium patent with the preservation number of CGMCC No.137, and is suitable for industrialization.

Disclosure of Invention

The invention mainly solves the technical problem of providing a rice planting method for simply improving the contents of trace elements Se and SOD.

In order to solve the technical problems, the invention adopts the technical scheme that:

a method for planting rice comprises the steps of seed treatment, seedling raising, seedling transplanting, fertilization, field management, pest control and harvesting, wherein the steps of:

applying an enhancer in the rice planting process, wherein the enhancer comprises bacillus cereus capable of producing SOD and selenium-containing fertilizer.

The fertilization can comprise applying base fertilizer and topdressing at the tillering stage, the flowering promoting stage, the flowering maintaining stage, the heading and flowering stage and the grain filling stage of the rice.

Optionally, the bacillus cereus and the selenium-containing fertilizer are applied simultaneously.

Optionally, the selenium-containing fertilizer is an organic selenium fertilizer or an inorganic selenium fertilizer.

Optionally, the selenium in the selenium-containing fertilizer is sodium selenate.

Optionally, the selenium-containing fertilizer is used in an amount of 1-2g selenium per acre.

Optionally, the preservation number of the bacillus cereus is CGMCC No. 137.

Optionally, the amount of the bacillus cereus is 50-150mL of bacillus cereus liquid per mu, and the concentration of the liquid is 1-2 hundred million CFU/mL. Preferably, the dosage of the bacillus cereus is 150mL of bacillus cereus liquid per mu, and the liquid concentration is 1 hundred million CFU/mL.

Optionally, the bacillus cereus and selenium-containing fertilizer are applied by spraying on the foliage and/or stems of the rice.

Optionally, the bacillus cereus and the selenium-containing fertilizer are applied at a flowering stage and/or a heading stage of the rice.

Before the reinforcing agent is sprayed, the reinforcing agent is diluted in multiple mode, the spraying is carried out for 1-3 times, the blade and the stem are uniformly attached by water as much as possible, and the reinforcing agent is sprayed again when raining in 4 hours after the spraying is carried out and the strong wind, the rain fog and the afternoon strong light time are avoided. Typically 10 am or 5 pm later.

The beneficial effects of the invention are mainly as follows:

1) the frequency of applying bacterial manure is less, the operation is simple, and manpower and material resources are saved.

2) Greatly improves the contents of trace elements Se and enzyme SOD in rice grains.

Detailed Description

The technical solutions of the present invention are described in detail below by specific embodiments, and it should be specifically noted that the following embodiments are only examples of the present invention, and do not limit the scope of the present invention, and all equivalent changes made by using the content of the present specification, or direct or indirect applications in other related technical fields are included in the scope of the present invention.

The inventive step of the present invention was explained by performing the synchronization experiment according to the basic principle of the comparative experiment.

Selecting 3 fields with basically the same soil and climate conditions for comparison experiment, one field is called a control field, the other two fields are called an experimental field 1 and an experimental field 2, the conditions are basically the same, including but not limited to the conditions of selenium content, form, soil texture, pH, moisture content and soil organic matter of the soil, the 3 fields are planted by using rice of the same variety, the planting density is the same, the 3 fields are all planted by adopting a conventional field rice planting method, and the method comprises seed treatment, seedling raising, seedling transplanting, fertilization, field management, pest control and harvesting, the fertilization comprises applying base fertilizer and topdressing at a tillering stage, a flowering promoting stage, a flowering preserving stage, a heading and a filling stage of the rice, and the difference is that the experimental field also applies a reinforcing agent in the rice planting process, wherein the reinforcing agent comprises bacillus cereus capable of generating SOD and a selenium-containing fertilizer, the preservation number of the bacillus cereus is CGMCC No.137, the bacillus cereus and the selenium-containing fertilizer are applied simultaneously, the selenium in the selenium-containing fertilizer is sodium selenate, the bacillus cereus and the selenium-containing fertilizer are sprayed and applied to leaf surfaces and stems of rice, and the application time of the bacillus cereus and the selenium-containing fertilizer is the flowering promoting period and the heading period of the rice; and the control field is correspondingly sprayed with the same amount of water instead of the bacillus cereus and the selenium-containing fertilizer.

The experimental field 1 the dosage of bacillus cereus is 50mL bacillus cereus liquid per mu, and the bacterial liquid concentration is 2 hundred million CFU/mL, the dosage of selenium-containing fertilizer is 1g selenium per mu, the experimental field 2 the dosage of bacillus cereus is 150mL bacillus cereus liquid per mu, and the bacterial liquid concentration is 1 hundred million CFU/mL, the dosage of selenium-containing fertilizer is 2g selenium per mu, before the reinforcing agent sprays, multiple dilution is carried out first, and spraying is carried out 1-3 times, and spraying should make blade, stem position all be evenly attached by water as far as possible, pay attention to avoid strong wind, rainy fog day and midday highlight period, need spray again if 4 hours after spraying rainy. Typically 10 am or 5 pm later.

After the rice is mature, selecting a proper node for harvesting, directly measuring and calculating the average SOD content and the selenium content in the rice seeds, detecting the SOD content by adopting the national standard GB/T5009.171-2003, and detecting the selenium by adopting GB/T5009.93-2017, wherein the results are as follows:

TABLE 1

The comparison shows that:

the planting method adopted by the invention can improve the content of SOD active enzyme and the content of trace element Se. Meanwhile, compared with the prior art, the fertilizer application and planting are simple. Among them, the effect of the experimental field 2 is better.

Claims

1. A planting method of rice rich in selenium and SOD is characterized by comprising the steps of seed treatment, seedling raising, seedling transplanting, fertilization, field management, pest control and harvesting, wherein the steps of:

2. The growing method of claim 1, wherein the bacillus cereus and the selenium-containing fertilizer are applied simultaneously.

3. The planting method according to claim 1 or 2, wherein the selenium-containing fertilizer is an organic selenium fertilizer or an inorganic selenium fertilizer.

4. The planting method of claim 1 or 2, wherein the selenium in the selenium-containing fertilizer is sodium selenate.

5. The planting method of claim 4, wherein the selenium-containing fertilizer is applied in an amount of 1-2g selenium per acre.

6. The planting method as claimed in claim 4 or 5, wherein the preservation number of the Bacillus cereus is CGMCC No. 137.

7. The planting method of claim 6, wherein the amount of the bacillus cereus is 50-150mL of bacillus cereus solution per mu, and the concentration of the bacillus cereus solution is 1-2 hundred million CFU/mL.

8. The planting method of claim 7, wherein the amount of the bacillus cereus is 150mL of bacillus cereus solution per mu, and the concentration of the bacillus cereus solution is 1 hundred million CFU/mL.

9. A method of growing as claimed in any preceding claim, wherein the site of application of the bacillus cereus and the selenium-containing fertilizer is the foliage and/or stem of rice.

10. The planting method of claim 9, wherein the bacillus cereus and the selenium-containing fertilizer are applied at the flowering and/or heading stages of rice.