CN103553828B - Liquid compound biological fertilizer with long shelf life as well as preparation method and application thereof - Google Patents
Liquid compound biological fertilizer with long shelf life as well as preparation method and application thereof Download PDFInfo
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- CN103553828B CN103553828B CN201310561591.5A CN201310561591A CN103553828B CN 103553828 B CN103553828 B CN 103553828B CN 201310561591 A CN201310561591 A CN 201310561591A CN 103553828 B CN103553828 B CN 103553828B
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Abstract
The invention relates to a liquid compound biological fertilizer with long shelf life as well as a preparation method and application thereof. The liquid compound biological bacterial fertilizer comprises the following components in parts by weight: 2-5 parts of microbial thalli, 80-130 parts of compound nutrients, 10-30 parts of thalli protective agents and 0.5-3.0 parts of additives, wherein the microbial thalli is metered in terms of the wet weight of deposition obtained after fermentation liquor is centrifugalized, the microbial thalli include bacillus thuringiensis, bacillus megaterium, bacillus mucilaginosus and bacillus amyloliquefaciens, and the total viable count achieves 5*10<8>-5*10<9>/mL. The liquid compound biological bacterial fertilizer disclosed by the invention can be used as a biological pesticide, can be directly applied to the spraying of plant surfaces or applied to seed soaking, root irrigation, and the like, has the effects of insect resistance, phosphate solubilization, potassium solubilization, organic matter solubilization, pest resistance, and the like and is long in shelf life.
Description
Technical field
The invention belongs to composite microbic bacterial fertilizer field, be specifically related to liquid composite bio-fertilizer of a kind of long quality guaranteed period and its preparation method and application.
Background technology
Conservation culture is the trend in the present age, and the development of agricultural is closely bound up with ecotope, healthy ecotope can provide strong support to ensure to agricultural, and can be important foundation and the guarantee of agricultural development, but owing to using chemical fertilizer or organic fertilizer for a long time, and Fertilization Structure the unreasonable pollution that causes China to be faced with agroecological environment and destruction, soil physical chemistry degradation situation and cause many serious problems.Become today of world agriculture developing direction in green agriculture, we should advocate energetically, developed and use microbial fertilizer, adhere to the road walking Sustainable development.
Micro organism composite fertilizer is based on beneficial microorganism, is equipped with a kind of novel green fertilizer of organic and inorganic composition.The vital movement of microorganism in composite fertilizer, can nutrient in activating soil, thus improve the supply of plant nutrient, plant growth substance is produced by the vital movement of microorganism, stimulating plant grows, and promotes that crop is to the absorption of nutritive element, some pathogenic microorganism of antagonism, alleviate crop pest, and increase yield.
Bacillus thuringiensis (Bacillus thuringiensis), can produce specific killing action to insect, have activity respectively to various insects such as lepidopteran, Diptera, Coleoptera, Hymenoptera, Homoptera, Orthoptera, Mallophagas.Bacillus megaterium (Bacillusmegaterium), can decompose insoluble phosphorus compound, and has stronger nitrite degradation ability.Colloid bacillus cereus (Bacillus mucilaginosus), the ecosystem mineral of the aluminum silicates such as decomposable asymmetric choice net feldspar, mica, make insoluble K, P, Si etc. in soil change solubility into and utilize for plant-growth.The growth of the biologically active substance such as growth hormone, phytokinin stimulating plant can also be produced.It is reported that colloid bacillus cereus has the effects such as volume increase, oil recovery enhancement, raising crop anti-adversity ability to various crop.In pure culture, also find that silicate bacteria can produce multiple amino acids, peptides simultaneously, and the physiologically active substance such as growth hormone, Plant hormones regulators,gibberellins, there is obvious restraining effect to phytopathogens such as cotton wilt, tobacco gray mold, wheat scabs, the generation of certain plants disease particularly some soil-borne diseases can be alleviated to a certain extent.Bacillus amyloliquefaciens (Bacillus amyloliquefaciens); organic matter decomposition ability is strong; meta-bolites enriches; producing multiplely has inhibiting material to plant pathogenic fungi; simultaneously higher to the restraint of spoilage organism and pathogenic bacteria, reproduction speed is fast, forms poly-Vetsin; form soil protection film, moisture retention is strong.
The composite microbic bacterial fertilizer sold in the market gets more and more, but most bacterial manure effect is comparatively single, and price is high, and most of liquid bacterial manure quality guaranteed period is short, thalline easy in inactivation.Liquid composite biological fertilizer of the present invention, the mode of production of low cost is adopted to produce, selecting function stem to make, product integrates pest-resistant, phosphorus decomposing, potassium decomposing, solution are organic thus reach the effect of crop yield, and with the addition of function salt, can be used as trace element and provide nutrition for plant, product also can be made to reach the system of high osmotic pressure, contribute to the yield, the stability that improve gemma, improve cortex construction, and increase its heat resist power.Meanwhile, with the addition of food sanitas, the vegetative activity of thalline in inhibition system, reaches the longer quality guaranteed period.
In marine alga, specific Sargassum polysaccharides, alginic acid, highly unsaturated fatty acids and multiple natural phant growth regulator, have very high biological activity, can the generation of the nonspecific activity factor in stimulating plant body, regulates Endogenous hormone balance.In marine alga containing natural phant growth regulator as: growth hormone, cytokinin-like substance and Plant hormones regulators,gibberellins etc., have very high biological activity, and in the ratio of various material and terrestrial plant, each hormone ratio is close.Lalgine in marine alga can reduce the surface tension of water, thin film is formed at plant surface, enlarge active surface, water-soluble substances ratio is made to be easier to enter vegetable cell through cauline leaf superficial cell film, make plant absorb nutritive ingredient in seaweed extracted liquor most effectively, if therefore liquid seaweed manure and sterilant, sterilant and chemical fertilizer used in combination, effect is better, sprinkling expense can be reduced, to agricultural chemicals and chemical fertilizer, there is synergism.Marine alga directly can make soil or by plant, soil be increased organic, activates the various beneficial microorganisms in soil, and these biologies can play a part catalyzer in plant-microbe metabolite circulation, and the biopotency of soil is increased.The metabolite of plants and soil microorganism can be plant provides more nutrient.Marine alga can be worked harmoniously with plant-soil ecosystem.Natural compounds such as the Sodium Alginate that it contains is natural soil amendment, the formation of soil aggregate can be promoted, improve soil internal void space, coordinate solid, liquid, gas proportions in soil, recover due to soil that over-burden and chemical pollution and the lac balance that loses, increase soil organisms vigor, promote the release of available nutrient, be conducive to root growth, improve the resistance of crop.
Summary of the invention
The object of this invention is to provide a kind ofly have that pest-resistant, phosphorus decomposing, potassium decomposing, solution are organic, insect pest effect and the liquid composite biological fertilizer of long quality-guarantee period and production method thereof.
To achieve these goals, the present invention adopts following technical scheme:
The composition of described liquid composite biological fertilizer comprises microbial cells, compound nutrients, bacteria protectant and additive; Its parts by weight consist of, microbial cells 2-5, compound nutrients 80-130, bacteria protectant 10-30, additive 0.5-3.0, wherein microbial cells be centrifugal in fermented liquid after precipitation weight in wet base.
The composition of described microbial cells comprises: bacillus thuringiensis (Bacillus thuringiensis), bacillus megaterium (Bacillusmegaterium), colloid bacillus cereus (Bacillus mucilaginosus), bacillus amyloliquefaciens (Bacillusamylolquefaciens), and wherein total viable count reaches 5 × 10
8-5 × 10
9individual/mL.
Preferably, in microbial cells, four kinds of bacterium viable count ratios are as follows: bacillus thuringiensis: bacillus megaterium: colloid bacillus cereus: bacillus amyloliquefaciens=1-2:2-3:2-3:1-2.
The parts by weight of described compound nutrients consist of:
Potassium primary phosphate 50-150,
EDTA-Ca25-30，
EDTA-Mg or EDTA-Mg and MgSO
47H
2both O mixture 50-80,
EDTA-Zn or EDTA-Zn and ZnSO
47H
2o or the two mixture 0.15-0.18,
EDTA-Mn or EDTA-Mn and MnSO
4h
2o or the two mixture 0.3-0.5,
EDTA-Fe0.1-0.3，
EDTA-Cu0.06-0.08，
Boric acid 0.1-0.3,
Humic acids 5-15,
Soybean protein 20-100,
Aminoacids complex 50,
Seaweed powder 20-100;
The parts by weight of described aminoacids complex consist of: L-Ala 6.0-6.5, arginine 6.5-6.8, aspartic acid 6.8-7.2, Gelucystine 2.2-2.5, glycine 3.2-3.5, Histidine 2.2-2.5, Methionin 6.4-6.5, methionine(Met) 3.0-3.2, phenylalanine 5-5.5, proline-4-4.2, Serine 8.4-8.5, Threonine 4.8-5.0, tryptophane 1.5-1.8, trorsine 14 .2-4.5, α-amino-isovaleric acid 7.4-7.5;
Described bacteria protectant is one or more in cell immobilization carrier, includes but not limited to diatomite, kaolin, talcum powder etc.;
Described additive is one or more in organic acid, includes but not limited to oxysuccinic acid, citric acid, lactic acid etc.
The production method of described liquid composite biological fertilizer is as follows:
(1) actication of culture and seed liquor preparation
Bacillus thuringiensis, bacillus megaterium, colloid bacillus cereus, Bacillus amyloliquefaciens strain carried out respectively activate and cultivate obtained seed liquor;
(2) fermentation culture
The seed liquor of cultured bacillus thuringiensis, bacillus megaterium, bacillus amyloliquefaciens is seeded to 5L fermentor tank with the inoculum size of 8%-10% respectively, fermention medium is No. 3 substratum, 37 DEG C ~ 40 DEG C, rotating speed 300rpm, ventilating ratio 1:1, collects fermented liquid after fermentation 15-18h;
The seed liquor of cultured colloid bacillus cereus is seeded to 5L fermentor tank with the inoculum size of 8%-10%, and fermention medium is No. 4 substratum, 37 DEG C ~ 40 DEG C, rotating speed 400rpm, ventilating ratio 1:1, collects fermented liquid after fermentation 15-18h;
Described No. 3 substratum composition: by percentage to the quality, 1.0%-1.2% glucose, 0.5%-0.6%K
2hPO
43H
2o, 0.3%-0.5%KH
2pO
4, 0.1%-0.2% urea, 0.4%-0.6% yeast powder, 0.35%-0.45 ‰ MgSO
47H
2o, all the other are water, pH7.0-7.2;
Described No. 4 substratum: by percentage to the quality, 1% sucrose, 0.5%K
2hPO
43H
2o, 0.5 ‰ yeast powders, 0.02 ‰ FeCl
36H
2o, 0.4 ‰ CaCO
3, 0.2 ‰ MgSO
47H
2o, all the other are water, pH7.0;
(3) microorganism collection is with fixing
Four kinds of fermented liquid mixing that step (2) is collected, 4 DEG C, 3000rpm-5000rpm, 8min-10min are centrifugal, supernatant discarded, collect thalline, the thalline sterilized water Eddy diffusion of mixed fermentation liquid cumulative volume 1/5-1/10, adds mixed fermentation liquid 5%-15%(W/V) bacteria protectant, magnetic stirring apparatus carries out absorption 30min-40min, or manually stir evenly, leave standstill 10-15min;
(4) the adding of compound nutrients and additive
Add compound nutrients and additive in the bacterium liquid obtained after step (3) is collected and fixed, be settled to centrifugal front mixed fermentation liquid and amass.
(5) filling
The liquid composite biological fertilizer of step (4) gained mycetome, nutrient substance, bacteria protectant and additive is added in the Plastic Bottle of uv sterilisation, pastes Product labelling, obtain liquid composite bio-fertilizer of the present invention.
Liquid composite biological fertilizer of the present invention can be used as biological pesticide, can directly apply to plant surface and spray, or is applied to seed soaking, filling root etc.
Application process: by liquid composite biological fertilizer dilution 200-300 of the present invention doubly, leave standstill 30min, then stir evenly, directly spray in kind of plant blade face; Or be directly used in seed soaking by after liquid composite biological fertilizer dilution 150-250 times of the present invention; Also can be used for filling with root by after liquid composite biological fertilizer dilution 100-200 times of the present invention, every mu of ground applies composite biological fertilizer 200mL-400mL.
Beneficial effect:
(1) the present invention adopts bacillus thuringiensis, bacillus megaterium, colloid bacillus cereus, bacillus amyloliquefaciens as the production bacterial strain of composite microbiological fertilizer, is equipped with the production technique of optimization, makes contained each function yeast viable count in finished product all reach 10
8individual/more than mL, is 10-15 times that common commercially available prod bacterium is dense, has high efficiency; The comprehensive functions such as bacterial strain can reach pest-resistant, phosphorus decomposing potassium, decomposition organic matter, adjustment soil pH.
(2) bacterial manure of the present invention is simultaneously composite with Organic-inorganic composite function nutrition element, for increasing soil fertility, improving crop growth environment, improvement soil and Promoting plant growth and serving active effect; Composite nutritive salt causes height to ooze system, and adds the additive such as organic acid and food preservatives, safety non-toxic, reduces system pH environment, strengthens the dormant state of microorganism, jointly reaches the vital movement suppressing thalline, the effect extended the shelf life; Liquid composite biological fertilizer of the present invention can preservation at least 6 months under the condition of room temperature lucifuge.
(2) in the present invention, bacterial manure is composite with Organic-inorganic composite function nutrition element, adds the trace element of Chelating state, plays between element the effect mutually coordinated, rely on and restrict.Due to the singularity of chelating salt structure added, electric charge in composite ion is made to be tending towards neutral, in soil, avoid cationic exchange and crop root surface can be arrived fast, more easily by Root Absorption, spray, on blade face, there is good mobility, easily absorbed by pore, can participate in the albumen synthesis of body in without the need to photosynthetic situation directly, its fertilizer efficiency is greater than the inorganic nitrogenous fertilizer adding separately inorganic salt or equivalent.It is reported, the fertilizer efficiency of the micro-fertilizer of Chelating state is 2 ~ 5 times of inorganic micro-fertilizer.
(3) bacterial manure of the present invention adds organic acid, safety non-toxic, reduces system pH environment, strengthens the dormant state of microorganism, jointly reaches the vital movement suppressing thalline, the effect extended the shelf life; Liquid composite biological fertilizer of the present invention can preservation at least 6 months under the condition of room temperature lucifuge.
(4) the present invention adopts cheap raw material as the substratum of thalline liquid state fermentation, adopts efficient fermentation process, reduces production cost, provide a kind of method preparing bio-bacterial manure of high efficiency low cost; The substratum of optimization of the present invention has the effect of fast fast-growing spore, makes thalline cultivate the gemma production rate that 12-15h just can reach 90%.
(4) composite biological fertilizer provided by the present invention, find in the planting experiment of the plants such as bracketplant, Plantula Brassicae chinensis, caraway, fertilising group is all obviously better than blank group in leaf length, root length, plant height, fresh weight, output etc., simultaneously can insect pest, regulates the pH of alkaline soil;
(5) contained by bacterial manure of the present invention, four kinds of production bacterial strains are gemma bacterioid, growth and breeding ability is vigorous, to the strong adaptability of environment, be easy to grow soil is decided at the higher level but not officially announced, when the zymotechnique that the present invention adopts and substratum make fermentable reach terminal, spore forming rate all reaches 90%-95%.
(6) the present invention with the addition of bacteria protectant as diatomite, kaolin etc., and make thalline in this material, be in dormant state between the shelf lives, Cell protection tolerates the high osmotic pressure of bacterial manure system better.When it is used, bacteria protectant can be dissolved very soon, and thalline Fast Stripping is rejuvenated, and thalline just can be made to depart from stripping unlike the compact structure such as rhombspar or the calcium carbonate material demand long period.
(7) in the present invention seaweed powder of adding containing organic substances such as indolylacetic acid, plant-growth kinetin, marine alga phenol, after coordinating function yeast and nutrient substance for more excellent drought resisting, anti-saline and alkaline infiltration can be played in plant husbandry, resist cold, sterilization and growth promoting function.
Embodiment
The present invention is described below by specific embodiment.Unless stated otherwise, technique means used in the present invention is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention only limited by claims.To those skilled in the art, under the prerequisite not deviating from essence of the present invention and scope, the various change carry out the material component in these embodiments and consumption or change also belong to protection scope of the present invention.
Embodiment 1
(1) preparation of substratum
No. 1 substratum: by percentage to the quality, 0.8%-1.2% peptone, 0.5%-1% yeast powder, 0.3%-1.0%NaCl, all the other are water, pH7.2-7.4.
No. 2 substratum: by percentage to the quality, 1%-2% N.F,USP MANNITOL or sucrose, 0.1 ‰-0.12 ‰ CaSO
42H
2o, 0.18 ‰-0.25 ‰ K
2hPO
4, 0.5%-0.6%CaCO
3, 0.15 ‰-0.2 ‰ MgSO
47H
2o, 0.2 ‰-0.25 ‰ NaCl, 1.4%-2% agar, all the other are water.
No. 3 substratum: by percentage to the quality, 1.0%-1.2% glucose, 0.5%-0.6%K
2hPO
43H
2o, 0.3%-0.5%KH
2pO
4, 0.1%-0.2% urea, 0.4%-0.6% yeast powder, 0.35%-0.45 ‰ MgSO
47H
2o, all the other are water, pH7.0-7.2.
No. 4 substratum: by percentage to the quality, 1% sucrose, 0.5%K
2hPO
43H
2o, 0.5 ‰ yeast powders, 0.02 ‰ FeCl
36H
2o, 0.4 ‰ CaCO
3, 0.2 ‰ MgSO
47H
2o, all the other are water, pH7.0.
(2) slant activation
Bacillus thuringiensis, bacillus megaterium, bacillus amyloliquefaciens are inoculated in No. 1 plate culture medium activation culture, by colloid bacillus cereus streak inoculation in No. 2 substratum activation culture.
(3) seed liquor preparation
The bacterium colony of picking bacillus thuringiensis, bacillus megaterium, bacillus amyloliquefaciens activation, is inoculated in No. 3 substratum respectively, preparation seed liquor; The bacterium colony of picking colloid bacillus cereus activation, is inoculated in No. 4 substratum, preparation seed liquor.
(4) fermentation culture
The seed liquor of cultured four bacterium is seeded to respective 5L fermentor tank, liquid amount 3L with the inoculum size of 10% respectively.Bacillus thuringiensis, bacillus megaterium, bacillus amyloliquefaciens, No. 3 substratum, 40 DEG C, rotating speed 300rpm, ventilating ratio 1:1; Colloid bacillus cereus, No. 4 substratum, 40 DEG C, rotating speed 400rpm, ventilating ratio 1:1; All collect fermented liquid after fermentation 15-18h.
After fermentation ends, after testing, each fermented liquid bacteria concentration all reaches 10
8individual/more than mL.
(5) four kinds of fermented liquid mixing of step (4) being collected, 4 DEG C, 5000rpm, 8min is centrifugal, supernatant discarded, collects thalline, the total fermented liquid 1/10(V/V of thalline) sterilized water Eddy diffusion, add total fermented liquid 5%(W/V) talcum powder, magnetic stirring apparatus carries out absorption 40min, leave standstill 15min;
(6) the adding of compound nutrients and additive
Collect in (5) and add compound nutrients and additive in the bacterium liquid fixedly obtained, being settled to centrifugal front mixed fermentation liquid and amassing.
The parts by weight of compound nutrients consist of: potassium primary phosphate 100, EDTA-Ca25, EDTA-Mg80, EDTA-Zn0.18, EDTA-Mn0.3, EDTA-Fe0.1, EDTA-Cu0.08, boric acid 0.3, humic acids 15, soybean protein 100, aminoacids complex 50, seaweed powder 20.
The parts by weight of aminoacids complex consist of: L-Ala 6.5, arginine 6.8, aspartic acid 6.8, Gelucystine 2.5, glycine 3.2, Histidine 2.2, Methionin 6.5, methionine(Met) 3.2, phenylalanine 5.5, proline-4, Serine 8.5, Threonine 4.8, tryptophane 1.8, trorsine 14 .5, α-amino-isovaleric acid 7.5.
Described additive is oxysuccinic acid.
(8) filling
Add in the Plastic Bottle of uv sterilisation by above-mentioned mixing liquid, aluminium foil sealing is covered plastic lid again, pastes Product labelling, obtains liquid composite microbiological fertilizer of the present invention.
Described in the present embodiment, the composition of liquid composite biological fertilizer comprises; Parts by weight consist of, microbial cells 2, compound nutrients 80, bacteria protectant 20, additive 0.5, wherein microbial cells be centrifugal in fermented liquid after precipitation weight in wet base.
Embodiment 2
(1) preparation of substratum
No. 1 substratum: by percentage to the quality, 1% peptone, 0.5% yeast powder, 0.3%NaCl, all the other are water, pH7.2;
No. 2 substratum: by percentage to the quality, 1% N.F,USP MANNITOL, 0.1 ‰ CaSO
42H
2o, 0.2 ‰ K
2hPO
4, 0.5%CaCO
3, 0.2 ‰ MgSO
47H
2o, 0.2 ‰ NaCl, 1.4% agar, all the other are water;
No. 3 substratum: by percentage to the quality, 1.0% glucose, 0.6%K
2hPO
4, 0.3%KH
2pO
4, 0.15% urea, 0.5% yeast powder, 0.4 ‰ MgSO
47H
2o, all the other are water, pH7.2
No. 4 substratum: by percentage to the quality, 1% sucrose, 0.5%K
2hPO
43H
2o, 0.5 ‰ yeast powders, 0.02 ‰ FeCl
36H
2o, 0.4 ‰ CaCO
3, 0.25 ‰ MgSO
47H
2o, all the other are water, pH7.0,
(2) slant activation
The each bacteria strain activation that 4 DEG C are preserved, after by bacillus thuringiensis, bacillus megaterium, bacillus amyloliquefaciens respectively streak inoculation on No. 1 plate culture medium, cultivate 1d for 30 DEG C-37 DEG C; By colloid bacillus cereus streak inoculation in No. 2 substratum, cultivate 1-2d for 25 DEG C-30 DEG C.
(3) seed liquor preparation
The bacterium colony of picking bacillus thuringiensis, bacillus megaterium, bacillus amyloliquefaciens activation, is inoculated in No. 3 substratum respectively, 37 DEG C, 200rpm, cultivates 16h;
The bacterium colony of picking colloid bacillus cereus activation, is inoculated in No. 4 substratum, 37 DEG C, 200rpm, cultivates 16h; Now the dense bacterium of the bacterium of four bacterium all reaches 10
8individual/mL.
(4) fermentation culture
The seed liquor of cultured four bacterium is seeded to respective 5L fermentor tank, liquid amount 3L with the inoculum size of 10% respectively.Bacillus thuringiensis, bacillus megaterium, bacillus amyloliquefaciens, No. 3 substratum, 37 DEG C, rotating speed 300rpm, ventilating ratio 1:1; Colloid bacillus cereus, No. 4 substratum, 37 DEG C, rotating speed 400rpm, ventilating ratio 1:1; All collect fermented liquid after fermentation 15-18h.
After fermentation ends, after testing, each fermented liquid bacteria concentration is: bacillus megaterium 8 × 10
8individual/mL, bacillus thuringiensis 1 × 10
9individual/mL, bacillus amyloliquefaciens 5 × 10
8individual/mL, colloid bacillus cereus 6 × 10
8individual/mL, each bacterium brood cell production rate all reaches 95%.
(5) microorganism collection is with fixing
Four kinds of fermented liquids step (3) collected mix with volume ratio 3:2:3:4,4 DEG C, 4000rpm, 10min is centrifugal, supernatant discarded, collects thalline, the total fermented liquid 1/5(V/V of thalline) sterilized water Eddy diffusion, add total fermented liquid 10%(W/V) diatomite, magnetic stirring apparatus carries out absorption 30min, leave standstill 10min;
(6) the adding of compound nutrients and additive
Collect in (5) and add compound nutrients and additive in the bacterium liquid fixedly obtained, being settled to centrifugal front mixed fermentation liquid and amassing.
The parts by weight of described compound nutrients consist of: potassium primary phosphate 150, EDTA-Ca28, MgSO
47H
2o+EDTA-Mg60, EDTA-Zn+ZnSO
47H
2o0.16, EDTA-Mn+MnSO
4h
2o0.4, EDTA-Fe0.2, EDTA-Cu0.07, boric acid 0.13, humic acids 10, soybean protein 40, aminoacids complex 50, seaweed powder 50.
Wherein the consisting of of aminoacids complex: mass fraction is, L-Ala 6.1, arginine 6.6, aspartic acid 7.0, Gelucystine 2.3, glycine 3.5, Histidine 2.4, Methionin 6.4, methionine(Met) 3.1, phenylalanine 5.0, proline-4 .2, Serine 8.4, Threonine 5.0, tryptophane 1.6, trorsine 14 .3, α-amino-isovaleric acid 7.4.
Described additive is oxysuccinic acid and citric acid 1:2 volume mixture.
(8) filling
Add in the Plastic Bottle of uv sterilisation by above-mentioned mixing liquid, aluminium foil sealing is covered plastic lid again, pastes Product labelling, obtains liquid composite microbiological fertilizer of the present invention.
Described in the present embodiment, the composition of liquid composite biological fertilizer comprises; Parts by weight consist of, microbial cells 5, compound nutrients 110, bacteria protectant 30, additive 3.0, wherein microbial cells be centrifugal in fermented liquid after precipitation weight in wet base.
The total viable count of liquid composite biological fertilizer of the present invention is 6.9 × 10
8individual/mL, pH4.0, ionic strength 80ms/cm.
By standing in the room temperature not higher than 30 DEG C for the liquid composite biological fertilizer preparing gained, lucifuge preservation, the total viable count of results of regular determination product, the total viable count of product did not have obvious decline within 3 months, do not decline to a great extent in 6 months, during preservation to 12 month, still required by compound national standard, be not less than 0.5 × 10
8individual/mL.
Total viable count of table 1 liquid composite biological fertilizer different storage time
Above-mentioned bacterial classification is bought certainly: bacillus thuringiensis (Bacillus thuringiensis): Guangdong Province's Culture Collection (5th floor, No. 100, Xianlie Middle Road, Guangzhou City Institute of Micro-biology of province laboratory building, postcode 510070), numbering GIM1.151; Bacillus megaterium (Bacillus megatherium): China General Microbiological culture presevation administrative center (No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, postcode 100101), numbering CGMCC1.223; Bacillus amyloliquefaciens: Chinese industrial Microbiological Culture Collection administrative center (No. 24, Road, Jiuxianqiao, Chaoyang District, Beijing City institute No. 6 building, postcode 100015), numbering CICC23845; Colloid bacillus cereus (Bacillus mucilaginosu): Chinese industrial Microbiological Culture Collection administrative center, numbering CICC23575.
Embodiment 3
The fermented liquid of four kinds of bacterium described in embodiment 2 is prepared according to the described method in (1) in embodiment 2-(4), by four kinds of fermented liquid equal-volume mixing of collecting, 4 DEG C, 3000rpm, 8min are centrifugal, supernatant discarded, collect thalline, the total fermented liquid 1/8(V/V of thalline) sterilized water Eddy diffusion, add total fermented liquid 15%(m/V) kaolin, magnetic stirring apparatus carries out absorption 30min, leaves standstill 10min; Add compound nutrients and additive, be settled to centrifugal front mixed fermentation liquid and amass.By the filling preservation of (8) described method in embodiment 1, meanwhile, not add the product of bacteria protectant and additive for contrasting.Its total viable count of results of regular determination.
The parts by weight of described compound nutrients consist of: potassium primary phosphate 50, EDTA-Ca30, MgSO
47H
2o+EDTA-Mg50, EDTA-Zn0.15, EDTA-Mn+MnSO
4h
2o0.5, EDTA-Fe0.3, EDTA-Cu0.06, boric acid 0.2, humic acids 5, soybean protein 20, aminoacids complex 50, seaweed powder 100.
Wherein aminoacids complex composition is with embodiment 2.
Described additive consists of lactic acid and citric acid 1:1 volume mixture.
Described in the present embodiment, the composition of liquid composite biological fertilizer comprises; Parts by weight consist of, microbial cells 3, compound nutrients 130, bacteria protectant 20, additive 1.5, wherein microbial cells be centrifugal in fermented liquid after precipitation weight in wet base.
The liquid bacterial manure of table 2 is at total viable count (individual/mL) of different times
After the present invention with the addition of bacteria protectant and additive, in 6 months, keep bacterium number significantly not reduce, and thalline is energetic, meets total viable count in 3 months that GB NY/T798-2004 require and be not less than 0.5 hundred million/mL.
Embodiment 4
Liquid composite biological fertilizer is prepared according to method described in embodiment 2, this bacterial manure is diluted 200 times, get the solution 200mL after dilution to apply and 300g soil is housed, is loaded on the flowerpot of 12 × 10cm in (punching at the bottom of basin), soak into after soil until solution, by the bracketplant plant young (unrooted suitable for growing way, 4 leaves, the long 6cm of leaf, average plant height 6cm, fresh weight 1g) insert in soil and cultivate, every day waters, and every 7d topdresses once, arrange blank group, when experimental group is applied fertilizer, control group applies equivalent tap water simultaneously.Routine observation two groups of plant growing ways, measure soil pH, plant is observed during 45d, measure the parameter such as plant height, leaf length, root length, fresh weight, all demonstrate clear superiority than blank: the well developed root system of fertilising plant, Ye Changyou rises appreciably, and newborn blade is also more than blank, and fresh weight gaining rate reaches 400%.Meanwhile, after fertilising, the soil is porous, and soil pH has obvious reduction than unmanured soil pH, and this is that this has good regulating effect to the pH of the saline-alkali soil of north of China because thalline has secreted acid in soil in process of growth.
Plant parameter during table 3 bracketplant plantation 45d
Table 4 fertilized soil pH compares with non-fertilized soil pH
Embodiment 4
Prepare liquid composite biological fertilizer according to method described in embodiment 2, product is diluted 250 times, spray the blade surface in Chinese cabbage, Plantula Brassicae chinensis, wild cabbage, broccoli, sprayed once every 7 days, obviously can reduce lepidoptera pest harm, as small cabbage moth.
Embodiment 5
Liquid composite biological fertilizer is prepared according to method described in embodiment 3, product is imposed on the crops such as tomato, capsicum, eggplant, cucumber, dilute 150 times and fill with root, every mu of former solution 200mL, after this impose once every 7d, dilute 250 times, after terminating in crop cycle, each crop yield all can improve more than 30%.
Claims (7)
1. a liquid composite biological fertilizer, the composition of described liquid composite biological fertilizer comprises microbial cells, compound nutrients, bacteria protectant and additive; Its parts by weight consist of, microbial cells 2-5, compound nutrients 80-130, bacteria protectant 10-30, additive 0.5-3.0, wherein microbial cells be centrifugal in fermented liquid after precipitation weight in wet base;
The composition of described microbial cells comprises: bacillus thuringiensis (Bacillus thuringiensis), bacillus megaterium (Bacillus megaterium), colloid bacillus cereus (Bacillus mucilaginosus), bacillus amyloliquefaciens (Bacillus amylolquefaciens), and wherein total viable count reaches 5 × 10
8-5 × 10
9individual/mL; In described microbial cells, four kinds of bacterium viable count ratios are as follows: bacillus thuringiensis: bacillus megaterium: colloid bacillus cereus: bacillus amyloliquefaciens=1-2:2-3:2-3:1-2;
The parts by weight of described compound nutrients consist of: potassium primary phosphate 50-150, EDTA-Ca25-30, EDTA-Mg or EDTA-Mg and MgSO
47H
2both O mixture 50-80, EDTA-Zn or EDTA-Zn and ZnSO
47H
2o or the two mixture 0.15-0.18, EDTA-Mn or EDTA-Mn and MnSO
4h
2o or the two mixture 0.3-0.5, EDTA-Fe0.1-0.3, EDTA-Cu0.06-0.08, boric acid 0.1-0.3, humic acids 5-15, soybean protein 20-100, aminoacids complex 50, seaweed powder 20-100;
Described bacteria protectant is one or more in cell immobilization carrier;
Described additive is one or more in organic acid;
The production method of described liquid composite biological fertilizer is as follows:
(1) actication of culture and seed liquor preparation: bacillus thuringiensis, bacillus megaterium, colloid bacillus cereus, Bacillus amyloliquefaciens strain carried out respectively activate and cultivate obtained seed liquor;
(2) fermentation culture: the seed liquor of cultured bacillus thuringiensis, bacillus megaterium, bacillus amyloliquefaciens is seeded to 5L fermentor tank with the inoculum size of 8%-10% respectively, fermention medium is No. 3 substratum, 37 DEG C ~ 40 DEG C, rotating speed 300rpm, ventilating ratio 1:1, collects fermented liquid after fermentation 15-18h; The seed liquor of cultured colloid bacillus cereus is seeded to 5L fermentor tank with the inoculum size of 8%-10%, and fermention medium is No. 4 substratum, 37 DEG C ~ 40 DEG C, rotating speed 400rpm, ventilating ratio 1:1, collects fermented liquid after fermentation 15-18h;
Described No. 3 substratum composition: by percentage to the quality, 1.0%-1.2% glucose, 0.5%-0.6%K
2hPO
43H
2o, 0.3%-0.5%KH
2pO
4, 0.1%-0.2% urea, 0.4%-0.6% yeast powder, 0.35%-0.45 ‰ MgSO
47H
2o, all the other are water, pH7.0-7.2;
Described No. 4 substratum: by percentage to the quality, 1% sucrose, 0.5%K
2hPO
43H
2o, 0.5 ‰ yeast powders, 0.02 ‰ FeCl
36H
2o, 0.4 ‰ CaCO
3, 0.2 ‰ MgSO
47H
2o, all the other are water, pH7.0;
(3) microorganism collection is with fixing: four kinds of fermented liquids step (2) collected mix, 4 DEG C, 3000rpm-5000rpm, 8min-10min are centrifugal, supernatant discarded, collect thalline, the thalline sterilized water Eddy diffusion of mixed fermentation liquid cumulative volume 1/5-1/10, adds the bacteria protectant of mixed fermentation liquid 5%-15% (W/V), magnetic stirring apparatus carries out absorption 30min-40min, or manually stir evenly, leave standstill 10-15min;
(4) the adding of compound nutrients and additive: add compound nutrients and additive in the bacterium liquid obtained after step (3) is collected and fixed, be settled to centrifugal before mixed fermentation liquid amass;
(5) filling: the liquid composite biological fertilizer of step (4) gained mycetome, nutrient substance, bacteria protectant and additive is added in the Plastic Bottle of uv sterilisation, paste Product labelling, obtain described liquid composite bio-fertilizer.
2. liquid composite biological fertilizer according to claim 1, is characterized in that, the parts by weight of described aminoacids complex consist of: L-Ala 6.0-6.5, arginine 6.5-6.8, aspartic acid 6.8-7.2, Gelucystine 2.2-2.5, glycine 3.2-3.5, Histidine 2.2-2.5, Methionin 6.4-6.5, methionine(Met) 3.0-3.2, phenylalanine 5-5.5, proline-4-4.2, Serine 8.4-8.5, Threonine 4.8-5.0, tryptophane 1.5-1.8, trorsine 14 .2-4.5, α-amino-isovaleric acid 7.4-7.5.
3. liquid composite biological fertilizer according to claim 1, is characterized in that, described bacteria protectant is the mixture of a kind of in diatomite, kaolin, talcum powder or at least two kinds.
4. liquid composite biological fertilizer according to claim 1, is characterized in that, described additive is the mixture of a kind of in oxysuccinic acid, lactic acid, citric acid or at least two kinds.
5., according to the production method of the arbitrary described liquid composite biological fertilizer of claim 1-4, comprise the steps:
(1) actication of culture and seed liquor preparation
Bacillus thuringiensis, bacillus megaterium, colloid bacillus cereus, Bacillus amyloliquefaciens strain carried out respectively activate and cultivate obtained seed liquor;
(2) fermentation culture
The seed liquor of cultured bacillus thuringiensis, bacillus megaterium, bacillus amyloliquefaciens is seeded to 5L fermentor tank with the inoculum size of 8%-10% respectively, fermention medium is No. 3 substratum, 37 DEG C ~ 40 DEG C, rotating speed 300rpm, ventilating ratio 1:1, collects fermented liquid after fermentation 15-18h;
The seed liquor of cultured colloid bacillus cereus is seeded to 5L fermentor tank with the inoculum size of 8%-10%, and fermention medium is No. 4 substratum, 37 DEG C ~ 40 DEG C, rotating speed 400rpm, ventilating ratio 1:1, collects fermented liquid after fermentation 15-18h;
Described No. 3 substratum composition: by percentage to the quality, 1.0%-1.2% glucose, 0.5%-0.6%K
2hPO
43H
2o, 0.3%-0.5%KH
2pO
4, 0.1%-0.2% urea, 0.4%-0.6% yeast powder, 0.35%-0.45 ‰ MgSO
47H
2o, all the other are water, pH7.0-7.2;
Described No. 4 substratum: by percentage to the quality, 1% sucrose, 0.5%K
2hPO
43H
2o, 0.5 ‰ yeast powders, 0.02 ‰ FeCl
36H
2o, 0.4 ‰ CaCO
3, 0.2 ‰ MgSO
47H
2o, all the other are water, pH7.0;
(3) microorganism collection is with fixing
Four kinds of fermented liquid mixing that step (2) is collected, 4 DEG C, 3000rpm-5000rpm, 8min-10min are centrifugal, supernatant discarded, collect thalline, the thalline sterilized water Eddy diffusion of mixed fermentation liquid cumulative volume 1/5-1/10, adds the bacteria protectant of mixed fermentation liquid 5%-15% (W/V), magnetic stirring apparatus carries out absorption 30min-40min, or manually stir evenly, leave standstill 10-15min;
(4) the adding of compound nutrients and additive
In the bacterium liquid that step (3) obtains, add nutrient substance and additive, be settled to centrifugal front mixed fermentation liquid and amass;
(5) filling
The liquid composite biological fertilizer of step (4) gained mycetome, nutrient substance, bacteria protectant and additive is added in the Plastic Bottle of uv sterilisation, pastes Product labelling, obtain described liquid composite bio-fertilizer.
6. the production method of liquid composite biological fertilizer according to claim 5, is characterized in that, comprise the steps:
(1) actication of culture and seed liquor preparation
Bacillus thuringiensis, bacillus megaterium, colloid bacillus cereus, Bacillus amyloliquefaciens strain carried out respectively activate and cultivate obtained seed liquor;
(2) fermentation culture
The seed liquor of cultured four bacterium is seeded to respective 5L fermentor tank, liquid amount 3L, bacillus thuringiensis, bacillus megaterium, bacillus amyloliquefaciens with the inoculum size of 10% respectively, No. 3 substratum, 37 DEG C, rotating speed 300rpm, ventilating ratio 1:1; Colloid bacillus cereus, No. 4 substratum, 37 DEG C, rotating speed 400rpm, ventilating ratio 1:1; All collect fermented liquid after fermentation 15-18h;
No. 3 substratum: by percentage to the quality, 1.0% glucose, 0.6%K
2hPO
4, 0.3%KH
2pO
4, 0.15% urea, 0.5% yeast powder, 0.4 ‰ MgSO
47H
2o, all the other are water, pH7.2;
No. 4 substratum: by percentage to the quality, 1% sucrose, 0.5%K
2hPO
43H
2o, 0.5 ‰ yeast powders, 0.02 ‰ FeCl
36H
2o, 0.4 ‰ CaCO
3, 0.25 ‰ MgSO
47H
2o, all the other are water, pH7.0;
After fermentation ends, after testing, each fermented liquid bacteria concentration is: bacillus megaterium 8 × 10
8individual/mL, bacillus thuringiensis 1 × 10
9individual/mL, bacillus amyloliquefaciens 5 × 10
8individual/mL, colloid bacillus cereus 6 × 10
8individual/mL, each bacterium brood cell production rate all reaches 95%;
(3) microorganism collection is with fixing
Four kinds of fermented liquids step (2) collected mix with volume ratio 3:2:3:4,4 DEG C, 4000rpm, 10min is centrifugal, supernatant discarded, collects thalline, the thalline sterilized water Eddy diffusion of total fermented liquid 1/5 (V/V), add the diatomite of total fermented liquid 10% (W/V), magnetic stirring apparatus carries out absorption 30min, leave standstill 10min;
(4) the adding of compound nutrients and additive
Collect in (3) and add compound nutrients and additive in the bacterium liquid fixedly obtained, being settled to centrifugal front mixed fermentation liquid and amassing;
The parts by weight of described compound nutrients consist of: potassium primary phosphate 150, EDTA-Ca28, MgSO
47H
2o+EDTA-Mg60, EDTA-Zn+ZnSO
47H
2o0.16, EDTA-Mn+MnSO
4h
2o0.4, EDTA-Fe0.2, EDTA-Cu0.07, boric acid 0.13, humic acids 10, soybean protein 40, aminoacids complex 50, seaweed powder 50;
Wherein the consisting of of aminoacids complex: mass fraction is, L-Ala 6.1, arginine 6.6, aspartic acid 7.0, Gelucystine 2.3, glycine 3.5, Histidine 2.4, Methionin 6.4, methionine(Met) 3.1, phenylalanine 5.0, proline-4 .2, Serine 8.4, Threonine 5.0, tryptophane 1.6, trorsine 14 .3, α-amino-isovaleric acid 7.4;
Described additive is oxysuccinic acid and citric acid 1:2 volume mixture;
(8) filling
Add in the Plastic Bottle of uv sterilisation by above-mentioned mixing liquid, aluminium foil sealing is covered plastic lid again, pastes Product labelling, obtains described liquid composite microbiological fertilizer;
The parts by weight of described liquid composite biological fertilizer consist of, microbial cells 5, compound nutrients 110, bacteria protectant 30, additive 3.0, wherein microbial cells be centrifugal in fermented liquid after precipitation weight in wet base.
7. the arbitrary described liquid composite biological fertilizer of claim 1-4 is as the purposes of biological pesticide.
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Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20100032855A (en) * | 2007-06-20 | 2010-03-26 | 울트라 바이오테크 리미티드 | Microbial formulation and method of using the same promote plant growth |
| KR20090057539A (en) * | 2007-12-03 | 2009-06-08 | (주)큐젠바이오텍 | Method for manufacturing of a liquid fertilizer using a see algae, ulva pertusa kjellman |
| CN103204732B (en) * | 2012-12-31 | 2015-02-25 | 天津北洋百川生物技术有限公司 | Composite microbial fertilizer containing polyglutamate high-producing strain, preparation method and application thereof |
| CN103030441B (en) * | 2012-12-31 | 2014-06-25 | 天津北洋百川生物技术有限公司 | Novel pest-resistant liquid compound microbial fertilizer |
| CN103172437B (en) * | 2012-12-31 | 2015-01-28 | 天津北洋百川生物技术有限公司 | Liquid compound microbial fertilizer containing polyglutamic acid, and preparation method and application thereof |
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