CN110100668A - The implantation methods of cereal crop rich in SOD - Google Patents

The implantation methods of cereal crop rich in SOD Download PDF

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Publication number
CN110100668A
CN110100668A CN201910521310.0A CN201910521310A CN110100668A CN 110100668 A CN110100668 A CN 110100668A CN 201910521310 A CN201910521310 A CN 201910521310A CN 110100668 A CN110100668 A CN 110100668A
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China
Prior art keywords
bacillus cereus
stage
bacillus
implantation methods
sod
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CN201910521310.0A
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Chinese (zh)
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CN110100668B (en
Inventor
周秋峰
于沐
曹辉
左红娟
张果果
蒋亚琴
付尧
刘程宏
夏冬
徐贺威
赵建国
黄长志
徐建伟
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Zhengzhou Institute Of Agriculture And Forestry Sciences
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Zhengzhou Institute Of Agriculture And Forestry Sciences
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C21/00—Methods of fertilising, sowing or planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/20—Cereals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates

Abstract

The present invention provides the cultural methods of the cereal crop rich in SOD.The cultural method of cereal crop rich in SOD of the invention includes: to apply to produce SOD bacillus cereus to cereal crop.Compared to traditional cultivation method, SOD bacillus cereus is produced by directly applying to cereal crop, the present invention improves with shockingly realizing cereal crop SOD content several times, moreover, yield also significantly improves.

Description

The implantation methods of cereal crop rich in SOD
Technical field
The present invention relates to a kind of implantation methods of cereal crop rich in SOD.
Background technique
The cereal crop plantation in China is still within the extensive mode comparatively fallen behind at present, as compatriots consume water Flat gradually upgrading rapidly increases the consumption demand of green, pollution-free, functional cereal crop.Superoxides discrimination Changing enzyme (SOD) has preferable scavenging effect for the free radical that body metabolism generates, therefore green, environmentally friendly, organic in culture The SOD content further promoted in cereal crop on the basis of cereal crop has good real value.
Production SOD bacillus cereus is interior raw fungal component, and there is more research in China Agricultural University, is mainly extended to fruit tree, It is greatly improved the SOD yield of host, but the specific rule that host regulates and controls is still not clear in bacterium.
There is document report to realize raising SOD by adjusting fertilizer at present, but the mechanism of fertilizers regulation crop and fruit SOD is inadequate Clear, regulating effect is simultaneously unstable, moreover, fertilizing operation is excessively cumbersome.
Summary of the invention
In order to solve the above technical problems, the technical solution used in the present invention includes: to apply to produce SOD wax to cereal crop Sample bacillus.Similar to the common application fertilizer in this field for promoting plant growth, application bacillus be as A kind of reinforcing agent improves the SOD content of wheat, soil, crop surface can be applied in, in seed, with the allusion quotation of northern cereal crop For type represents wheat, applying the bacillus cereus can carry out at least one following plantation stage:
The Seeds preprocess stage, seedling stage, the jointing stage, chooses flag phase, heading stage, milk stage at sowing time；
For example, in the Seeds preprocess stage, choosing flag phase and heading stage and applying the bacillus cereus.
Optionally, in each plantation stage, the operation of the bacillus cereus is applied, is carried out 1-2 times；For example, The operation for applying the bacillus cereus, the Seeds preprocess stage and/or choose the flag phase carry out 1 time, heading stage repeat into Row 2 times.
Optionally, the operation for applying the bacillus cereus, including: in the Seeds preprocess stage will be containing the waxy bud The solution of spore bacillus is realized by way of with seed spice or immersion seed (bacillus concentration can be hundred million CFU/mL of 1-2) The application, the time for impregnating seed can be for 10-30h, and for example 1 day.
Optionally, the operation for applying the bacillus cereus includes: to spill to the stem and/or foliar spray of wheat choosing the flag phase Solution containing the bacillus cereus (bacillus concentration can be hundred million CFU/mL of 0.1-0.2)；Containing the bacillus cereus Solution, also containing 1% urea, 1% ammonium sulfate, 2% calcium superphosphate and 0.1% ammonium molybdate.
Optionally, the operation for applying the bacillus cereus includes: the fringe, stem and/or foliar spray to wheat at heading stage Spill the solution containing the bacillus cereus (bacillus concentration can be hundred million CFU/mL of 0.1-0.2)；Containing the waxy gemma bar The solution of bacterium, also containing 1% urea, 1% ammonium sulfate, 2% calcium superphosphate, 0.1% ammonium molybdate and 0.1% boron Sand.
Optionally, in the base fertilizer applied before winter sowing, contain per acre: purity nitrogen 15kg or so；Phosphorus is with P2O5Meter, 5kg or so； Potassium is with K2O meter, 15kg or so.
Optionally, it into after period of seedling establishment, applies fertilizer per acre: purity nitrogen 15kg or so；Phosphorus is with P2O5Meter, 2.5kg or so.
Optionally, it is CGMCC NO.0137, CGMCC NO.0760, CGMCC that the bacillus cereus, which is selected from deposit number, The bacterial strain of at least one of NO.0761 or CGMCC NO.0175.
The beneficial effect comprise that
It is studied through applicant, compares traditional cultivation method, produce the waxy gemma bar of SOD by directly applying to cereal crop Bacterium, by standard method detection SOD discovery, the present invention improves with shockingly realizing cereal crop SOD content several times, moreover, Yield also significantly improves, this be it is very very difficult, have great market value.
Specific embodiment
The lower mask body demonstration present invention, each embodiment are planted referring generally to the big thinking of the prior art, are then existed Using national standard GB/T5009.171-2003 detection SOD content etc. under the same terms, unless otherwise instructed, agents useful for same is city It sells or open approach is retrievable, the big principle such as crop growth period, production control and method also follow convention and its management-control method.
Embodiment 1:
Application deposit number is the bacillus cereus of CGMCC NO.0137 to improve the SOD content in wheat seed stage by stage:
Before winter sowing, apply nitrogen, phosphorus, potassium base fertilizer to soil, per acre 15kg containing purity nitrogen or so, phosphorus (P in base fertilizer2O5)5kg Left and right, potassium (K2O) 15kg or so；
Wheat seed is impregnated in the aqueous solution of the bacillus (bacillus concentration is hundred million CFU/mL of 1-2) in advance It 1 day, is then sowed after seed is dry；
Wheat enters top dressing after period of seedling establishment, adds purity nitrogen 15kg or so, phosphorus (P per acre2O5) 2.5kg or so；
After choosing the flag phase into wheat, sprinkling bacterial manure water to wheat cauline leaf is sufficiently soaked, and the bacillus is contained in bacterial manure water Hundred million CFU/mL of 0.1-0.2, also containing urea 1%, the calcium superphosphate of ammonium sulfate 1%, 2%, 0.1% ammonium molybdate；
Into after heading stage, sprinkling bacterial manure water to wheatear, stem, leaf surface are soaked, and the bacillus is contained in bacterial manure water Hundred million CFU/mL of 0.1-0.2, also containing urea 1%, the calcium superphosphate of ammonium sulfate 1%, 2%, 0.1% ammonium molybdate, 0.1% borax, Spring application operate in the early stage, the later period respectively does 1 time；
Other growth periods and specific operation, are carried out by this field routine principle, including, suitable node is selected after mature It is gathered in, direct measuring wheat seed is averaged SOD content, and then convert per mu yield.Calculated, average SOD content is 125U/g, per mu yield For 602kg.
Embodiment 2:
Application deposit number is the bacillus cereus of CGMCC NO.0760 to improve the SOD content in wheat seed stage by stage:
Before winter sowing, apply nitrogen, phosphorus, potassium base fertilizer to soil, per acre 15kg containing purity nitrogen or so, phosphorus (P in base fertilizer2O5)5kg Left and right, potassium (K2O) 15kg or so；
Wheat seed is impregnated in the aqueous solution of the bacillus (bacillus concentration is hundred million CFU/mL of 1-2) in advance It 1 day, is then sowed after seed is dry；
Wheat enters top dressing after period of seedling establishment, adds purity nitrogen 15kg or so, phosphorus (P per acre2O5) 2.5kg or so；
After choosing the flag phase into wheat, sprinkling bacterial manure water to wheat cauline leaf is sufficiently soaked, and the bacillus is contained in bacterial manure water Hundred million CFU/mL of 0.1-0.2, also containing urea 1%, the calcium superphosphate of ammonium sulfate 1%, 2%, 0.1% ammonium molybdate；
Into after heading stage, sprinkling bacterial manure water to wheatear, stem, leaf surface are soaked, and the bacillus is contained in bacterial manure water Hundred million CFU/mL of 0.1-0.2, also containing urea 1%, the calcium superphosphate of ammonium sulfate 1%, 2%, 0.1% ammonium molybdate, 0.1% borax, Spring application operate in the early stage, the later period respectively does 1 time；
Other growth periods and specific operation, are carried out by this field routine principle, including, suitable node is selected after mature It is gathered in, direct measuring wheat seed is averaged SOD content, and then convert per mu yield.Calculated, average SOD content is 105U/g, per mu yield For 610kg.
Embodiment 3:
Application deposit number is the bacillus cereus of CGMCC NO.0761 to improve the SOD content in wheat seed stage by stage:
Before winter sowing, apply nitrogen, phosphorus, potassium base fertilizer to soil, per acre 15kg containing purity nitrogen or so, phosphorus (P in base fertilizer2O5)5kg Left and right, potassium (K2O) 15kg or so；
Wheat seed is impregnated in the aqueous solution of the bacillus (bacillus concentration is hundred million CFU/mL of 1-2) in advance It 1 day, is then sowed after seed is dry；
Wheat enters top dressing after period of seedling establishment, adds purity nitrogen 15kg or so, phosphorus (P per acre2O5) 2.5kg or so；
After choosing the flag phase into wheat, sprinkling bacterial manure water to wheat cauline leaf is sufficiently soaked, and the bacillus is contained in bacterial manure water Hundred million CFU/mL of 0.1-0.2, also containing urea 1%, the calcium superphosphate of ammonium sulfate 1%, 2%, 0.1% ammonium molybdate；
Into after heading stage, sprinkling bacterial manure water to wheatear, stem, leaf surface are soaked, and the bacillus is contained in bacterial manure water Hundred million CFU/mL of 0.1-0.2, also containing urea 1%, the calcium superphosphate of ammonium sulfate 1%, 2%, 0.1% ammonium molybdate, 0.1% borax, Spring application operate in the early stage, the later period respectively does 1 time；
Other growth periods and specific operation, are carried out by this field routine principle, including, suitable node is selected after mature It is gathered in, direct measuring wheat seed is averaged SOD content, and then convert per mu yield.Calculated, average SOD content is 94U/g, per mu yield For 580kg.
Embodiment 4:
Application deposit number is the bacillus cereus of CGMCC NO.0175 to improve the SOD content in wheat seed stage by stage:
Before winter sowing, apply nitrogen, phosphorus, potassium base fertilizer to soil, per acre 15kg containing purity nitrogen or so, phosphorus (P in base fertilizer2O5)5kg Left and right, potassium (K2O) 15kg or so；
Wheat seed is impregnated in the aqueous solution of the bacillus (bacillus concentration is hundred million CFU/mL of 1-2) in advance It 1 day, is then sowed after seed is dry；
Wheat enters top dressing after period of seedling establishment, adds purity nitrogen 15kg or so, phosphorus (P per acre2O5) 2.5kg or so；
After choosing the flag phase into wheat, sprinkling bacterial manure water to wheat cauline leaf is sufficiently soaked, and the bacillus is contained in bacterial manure water Hundred million CFU/mL of 0.1-0.2, also containing urea 1%, the calcium superphosphate of ammonium sulfate 1%, 2%, 0.1% ammonium molybdate；
Into after heading stage, sprinkling bacterial manure water to wheatear, stem, leaf surface are soaked, and the bacillus is contained in bacterial manure water Hundred million CFU/mL of 0.1-0.2, also containing urea 1%, the calcium superphosphate of ammonium sulfate 1%, 2%, 0.1% ammonium molybdate, 0.1% borax, Spring application operate in the early stage, the later period respectively does 1 time；
Other growth periods and specific operation, are carried out by this field routine principle, including, suitable node is selected after mature It is gathered in, direct measuring wheat seed is averaged SOD content, and then convert per mu yield.Calculated, average SOD content is 87U/g, per mu yield For 570kg.
Comparative example:
Each operation is only that each step does not apply bacillus, is calculated that averagely SOD contains referring to above-described embodiment, difference Amount is 11U/g, per mu yield 515kg.

Claims (10)

1. the implantation methods of the cereal crop rich in SOD, this method comprises:
It is applied to cereal crop and produces SOD bacillus cereus.
2. implantation methods as described in claim 1, characterized in that the cereal crop is wheat.
3. implantation methods as claimed in claim 1 or 2, characterized in that apply the bacillus cereus be it is following at least What one plantation stage carried out:
The Seeds preprocess stage, seedling stage, the jointing stage, chooses flag phase, heading stage, milk stage at sowing time；
For example, in the Seeds preprocess stage, choosing flag phase and heading stage and applying the bacillus cereus.
4. implantation methods as claimed in claim 3, it is characterized in that: applying the waxy gemma in each plantation stage The operation of bacillus carries out 1-2 times；
For example, applying the operation of the bacillus cereus, in the Seeds preprocess stage and/or flag phase progress 1 time is chosen, is being eared Phase repeats 2 times.
5. implantation methods as described in claim 3 or 4, characterized in that the operation for applying the bacillus cereus, in seed Pretreatment stage include: by the solution containing the bacillus cereus, by with seed spice or impregnate seed (bacillus is dense Degree can be hundred million CFU/mL of 1-2) form, realize the application, impregnate seed time can be 10-30h, for example 1 day.
6. implantation methods as described in claim 3 or 4, characterized in that flag is being chosen in the operation for applying the bacillus cereus Phase includes: to spill stem from the solution containing the bacillus cereus to wheat and/or foliar spray (bacillus concentration can be 0.1-0.2 Hundred million CFU/mL)；Solution containing the bacillus cereus, also containing 1% urea, 1% ammonium sulfate, 2% peroxophosphoric acid Calcium and 0.1% ammonium molybdate.
7. implantation methods as described in claim 3 or 4, characterized in that the operation for applying the bacillus cereus is being eared Phase includes: to spill fringe from the solution containing the bacillus cereus to wheat, stem and/or foliar spray (bacillus concentration can be 0.1- 0.2 hundred million CFU/mL)；Solution containing the bacillus cereus, also containing 1% urea, 1% ammonium sulfate, 2% cross phosphorus Sour calcium, 0.1% ammonium molybdate and 0.1% borax.
8. the implantation methods as described in any first claim, characterized in that in the base fertilizer applied before winter sowing, per acre Contain: purity nitrogen 15kg or so；Phosphorus is with P2O5Meter, 5kg or so；Potassium is with K2O meter, 15kg or so.
9. the implantation methods as described in any first claim, characterized in that after entering period of seedling establishment, apply fertilizer per acre: purity nitrogen 15kg or so；Phosphorus is with P2O5Meter, 2.5kg or so.
10. the implantation methods as described in any first claim, it is characterized in that: the bacillus cereus is selected from deposit number The bacterial strain of at least one of CGMCC NO.0137, CGMCC NO.0760, CGMCC NO.0761 or CGMCC NO.0175.
CN201910521310.0A 2019-06-17 2019-06-17 Planting method of cereal crops rich in SOD Active CN110100668B (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110915581A (en) * 2019-11-27 2020-03-27 张家界鱼泉生态农业开发有限公司 Planting method of rice rich in selenium and SOD (superoxide dismutase)
CN115152548A (en) * 2022-07-20 2022-10-11 湖北富民梦农业实业有限公司 SOD (superoxide dismutase) colored wheat and production method thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1082605A (en) * 1993-04-21 1994-02-23 北京农业大学 A kind of genus bacillus and production method thereof that produces superoxide-dismutase
CN1807589A (en) * 2006-01-04 2006-07-26 中国农业大学 Method for cultivating superoxide disproportionation enzyme cucumber and its special biological agent
CN102742429A (en) * 2011-04-21 2012-10-24 张艺赢 Superoxide dismutase (SOD) wheat production method
CN106834192A (en) * 2017-03-24 2017-06-13 山东五福生生态工程有限公司 Bacillus cereus solid fermentation method and its tunning and application
CN107593334A (en) * 2017-08-28 2018-01-19 常熟新晟现代农业科技有限公司 A kind of implantation methods of high SOD contents rice

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1082605A (en) * 1993-04-21 1994-02-23 北京农业大学 A kind of genus bacillus and production method thereof that produces superoxide-dismutase
CN1807589A (en) * 2006-01-04 2006-07-26 中国农业大学 Method for cultivating superoxide disproportionation enzyme cucumber and its special biological agent
CN102742429A (en) * 2011-04-21 2012-10-24 张艺赢 Superoxide dismutase (SOD) wheat production method
CN106834192A (en) * 2017-03-24 2017-06-13 山东五福生生态工程有限公司 Bacillus cereus solid fermentation method and its tunning and application
CN107593334A (en) * 2017-08-28 2018-01-19 常熟新晟现代农业科技有限公司 A kind of implantation methods of high SOD contents rice

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110915581A (en) * 2019-11-27 2020-03-27 张家界鱼泉生态农业开发有限公司 Planting method of rice rich in selenium and SOD (superoxide dismutase)
CN115152548A (en) * 2022-07-20 2022-10-11 湖北富民梦农业实业有限公司 SOD (superoxide dismutase) colored wheat and production method thereof

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