CN110100668B - Planting method of cereal crops rich in SOD - Google Patents

Planting method of cereal crops rich in SOD Download PDF

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Publication number
CN110100668B
CN110100668B CN201910521310.0A CN201910521310A CN110100668B CN 110100668 B CN110100668 B CN 110100668B CN 201910521310 A CN201910521310 A CN 201910521310A CN 110100668 B CN110100668 B CN 110100668B
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bacillus cereus
stage
bacillus
wheat
sod
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CN110100668A (en
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周秋峰
于沐
曹辉
左红娟
张果果
蒋亚琴
付尧
刘程宏
夏冬
徐贺威
赵建国
黄长志
徐建伟
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Zhengzhou Institute Of Agriculture And Forestry Sciences
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Zhengzhou Institute Of Agriculture And Forestry Sciences
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C21/00—Methods of fertilising, sowing or planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/20—Cereals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Soil Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Wood Science & Technology (AREA)
  • Botany (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Agronomy & Crop Science (AREA)
  • Microbiology (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Dentistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention provides a method for cultivating cereal crops rich in SOD. The cultivation method of the cereal crops rich in SOD comprises the following steps: SOD-producing Bacillus cereus was applied to cereal crops. Compared with the traditional cultivation method, the invention surprisingly realizes the increase of the SOD content of the cereal crops by a plurality of times and also obviously improves the yield by directly applying the SOD-producing bacillus cereus to the cereal crops.

Description

Planting method of cereal crops rich in SOD
Technical Field
The invention relates to a method for planting cereal crops rich in SOD.
Background
At present, cereal crop planting in China is still in a relatively laggard extensive mode, and with the gradual upgrade of consumption level of Chinese people, the consumption demand of green, pollution-free and functional cereal crops is rapidly increased. Superoxide dismutase (SOD) has a good scavenging effect on free radicals generated by body metabolism, so that the SOD content in cereal crops is further increased on the basis of culturing green, environment-friendly and organic cereal crops, and the SOD has a good practical value.
The SOD-producing Bacillus cereus is an endophytic symbiotic bacterium, has more researches at China university of agriculture, is mainly popularized in fruit trees, can greatly improve the SOD yield of hosts, and has unclear specific regulation and control rule of the bacterium on the hosts.
At present, the SOD is improved by regulating the fertilizer, but the mechanism of regulating the SOD of the crop fruits by the fertilizer is not clear enough, the regulating effect is not stable, and the fertilizing operation is too complicated.
Disclosure of Invention
In order to solve the technical problems, the technical scheme adopted by the invention comprises the following steps: SOD-producing Bacillus cereus was applied to cereal crops. Similar to the application of fertilizers for promoting crop growth common in the art, the application of bacillus cereus is an enhancer for increasing the SOD content of wheat, and can be applied to soil, crop surfaces, seeds, as exemplified by wheat, which is a typical representative of northern cereal crops, in at least one of the following planting stages:
a seed pretreatment stage, a sowing stage, a seedling stage, a jointing stage, a flag picking stage, a heading stage and a milk stage;
for example, the Bacillus cereus is applied during the seed pretreatment stage, the flag stage, and the heading stage.
Optionally, applying the bacillus cereus for 1-2 times at each of said planting stages; for example, the application of the Bacillus cereus is performed 1 time in the seed pretreatment stage and/or the heading stage, and repeated 2 times in the heading stage.
Optionally, the operation of applying the bacillus cereus comprises, in a seed pre-treatment stage: the application is achieved by mixing the solution containing the bacillus cereus with the seed or soaking the seed (bacillus concentration may be 1-2 hundred million CFU/mL) for a period of 10-30h, such as 1 day.
Optionally, the operation of administering the bacillus cereus comprises, during the flag stage: spraying a solution containing the Bacillus cereus (Bacillus concentration can be 0.1-0.2 hundred million CFU/mL) on the stem and/or leaf of wheat; the solution containing the bacillus cereus can also contain 1% of urea, 1% of ammonium sulfate, 2% of calcium superphosphate and 0.1% of ammonium molybdate.
Optionally, the operation of applying the bacillus cereus comprises, during the heading phase: spraying a solution containing the bacillus cereus (bacillus concentration can be 0.1-0.2 hundred million CFU/mL) on the ears, stems and/or leaves of the wheat; the solution containing the bacillus cereus can also contain 1% of urea, 1% of ammonium sulfate, 2% of calcium superphosphate, 0.1% of ammonium molybdate and 0.1% of borax.
Optionally, the base fertilizer applied before sowing in winter contains per mu: about 15kg of pure nitrogen; phosphorus as P2O5About 5kg in weight; k is potassium2About 15kg of O.
Optionally, after a green turning period, fertilizing per mu: about 15kg of pure nitrogen; phosphorus as P2O5About 2.5 kg.
Optionally, the bacillus cereus is selected from at least one strain with the preservation number of CGMCC NO.0137, CGMCC NO.0760, CGMCC NO.0761 or CGMCC NO. 0175.
The beneficial effects of the invention include:
through the research of the applicant, compared with the traditional planting method, the SOD is directly applied to the cereal crops to produce the SOD waxy bacilli, and the SOD is detected according to the standard method, so that the invention surprisingly realizes the purpose of improving the SOD content of the cereal crops by multiple times, obviously improves the yield, is very difficult and has great market value.
Detailed Description
The present invention is specifically demonstrated below, and the examples generally refer to the big idea of the prior art for planting, and then detect the SOD content and the like under the same conditions by using the national standard GB/T5009.171-2003, if no specific description is given, all the reagents are available on the market or publicly available, and the big principles and methods of crop growth period, production control and the like also follow the general principles and management methods thereof.
Example 1:
the bacillus cereus with the preservation number of CGMCC NO.0137 is applied in stages to improve the SOD content in wheat seeds:
before sowing in winter, applying nitrogen, phosphorus and potassium base fertilizers to soil, wherein each mu of base fertilizer contains about 15kg of pure nitrogen and phosphorus (P)2O5) About 5kg, potassium (K)2O) about 15 kg;
soaking wheat seeds in the aqueous solution of the bacillus (the concentration of the bacillus is 1-2 hundred million CFU/mL) for 1 day in advance, and then sowing the seeds after the seeds are dried;
after wheat is in the green turning stage, topdressing is carried out, about 15kg of pure nitrogen and phosphorus (P) are added per mu2O5) About 2.5 kg;
after the wheat leaves are picked, spraying bacterial manure water until the wheat leaves are fully wetted, wherein the bacterial manure water contains 0.1-0.2 hundred million CFU/mL of bacillus, 1% of urea, 1% of ammonium sulfate, 2% of calcium superphosphate and 0.1% of ammonium molybdate;
after entering the heading stage, spraying bacterial manure water until the surfaces of wheat ears, stems and leaves are wet, wherein the bacterial manure water contains 0.1-0.2 hundred million CFU/mL of bacillus, 1% of urea, 1% of ammonium sulfate, 2% of calcium superphosphate, 0.1% of ammonium molybdate and 0.1% of borax, and the spraying and fertilizing operations are respectively carried out for 1 time in the initial stage and the later stage;
other growth periods and specific operations are carried out according to the conventional principle in the field, including that after the wheat is matured, a proper node is selected for harvesting, the average SOD content of the wheat seeds is directly measured and calculated, and then the yield per mu is converted. Through measurement and calculation, the average SOD content is 125U/g, and the yield per mu is 602 kg.
Example 2:
the bacillus cereus with the preservation number of CGMCC NO.0760 is applied in stages to improve the SOD content in wheat seeds:
before sowing in winter, applying nitrogen, phosphorus and potassium base fertilizers to soil, wherein each mu of base fertilizer contains about 15kg of pure nitrogen and phosphorus (P)2O5) About 5kg, potassium (K)2O) about 15 kg;
soaking wheat seeds in the aqueous solution of the bacillus (the concentration of the bacillus is 1-2 hundred million CFU/mL) for 1 day in advance, and then sowing the seeds after the seeds are dried;
after wheat is in the green turning stage, topdressing is carried out, about 15kg of pure nitrogen and phosphorus (P) are added per mu2O5) About 2.5 kg;
after the wheat leaves are picked, spraying bacterial manure water until the wheat leaves are fully wetted, wherein the bacterial manure water contains 0.1-0.2 hundred million CFU/mL of bacillus, 1% of urea, 1% of ammonium sulfate, 2% of calcium superphosphate and 0.1% of ammonium molybdate;
after entering the heading stage, spraying bacterial manure water until the surfaces of wheat ears, stems and leaves are wet, wherein the bacterial manure water contains 0.1-0.2 hundred million CFU/mL of bacillus, 1% of urea, 1% of ammonium sulfate, 2% of calcium superphosphate, 0.1% of ammonium molybdate and 0.1% of borax, and the spraying and fertilizing operations are respectively carried out for 1 time in the initial stage and the later stage;
other growth periods and specific operations are carried out according to the conventional principle in the field, including that after the wheat is matured, a proper node is selected for harvesting, the average SOD content of the wheat seeds is directly measured and calculated, and then the yield per mu is converted. Through measurement and calculation, the average SOD content is 105U/g, and the yield per mu is 610 kg.
Example 3:
the bacillus cereus with the preservation number of CGMCC NO.0761 is applied in stages to improve the SOD content in wheat seeds:
before sowing in winter, applying nitrogen, phosphorus and potassium base fertilizers to soil, wherein each mu of base fertilizer contains about 15kg of pure nitrogen and phosphorus (P)2O5) About 5kg, potassium (K)2O) about 15 kg;
soaking wheat seeds in the aqueous solution of the bacillus (the concentration of the bacillus is 1-2 hundred million CFU/mL) for 1 day in advance, and then sowing the seeds after the seeds are dried;
after wheat is in the green turning stage, topdressing is carried out, about 15kg of pure nitrogen and phosphorus (P) are added per mu2O5) About 2.5 kg;
after the wheat leaves are picked, spraying bacterial manure water until the wheat leaves are fully wetted, wherein the bacterial manure water contains 0.1-0.2 hundred million CFU/mL of bacillus, 1% of urea, 1% of ammonium sulfate, 2% of calcium superphosphate and 0.1% of ammonium molybdate;
after entering the heading stage, spraying bacterial manure water until the surfaces of wheat ears, stems and leaves are wet, wherein the bacterial manure water contains 0.1-0.2 hundred million CFU/mL of bacillus, 1% of urea, 1% of ammonium sulfate, 2% of calcium superphosphate, 0.1% of ammonium molybdate and 0.1% of borax, and the spraying and fertilizing operations are respectively carried out for 1 time in the initial stage and the later stage;
other growth periods and specific operations are carried out according to the conventional principle in the field, including that after the wheat is matured, a proper node is selected for harvesting, the average SOD content of the wheat seeds is directly measured and calculated, and then the yield per mu is converted. Through measurement and calculation, the average SOD content is 94U/g, and the yield per mu is 580 kg.
Example 4:
the bacillus cereus with the preservation number of CGMCC NO.0175 is applied in stages to improve the SOD content in wheat seeds:
before sowing in winter, applying nitrogen, phosphorus and potassium base fertilizers to soil, wherein each mu of base fertilizer contains about 15kg of pure nitrogen and phosphorus (P)2O5) About 5kg, potassium (K)2O) about 15 kg;
soaking wheat seeds in the aqueous solution of the bacillus (the concentration of the bacillus is 1-2 hundred million CFU/mL) for 1 day in advance, and then sowing the seeds after the seeds are dried;
after wheat is in the green turning stage, topdressing is carried out, about 15kg of pure nitrogen and phosphorus (P) are added per mu2O5) About 2.5 kg;
after the wheat leaves are picked, spraying bacterial manure water until the wheat leaves are fully wetted, wherein the bacterial manure water contains 0.1-0.2 hundred million CFU/mL of bacillus, 1% of urea, 1% of ammonium sulfate, 2% of calcium superphosphate and 0.1% of ammonium molybdate;
after entering the heading stage, spraying bacterial manure water until the surfaces of wheat ears, stems and leaves are wet, wherein the bacterial manure water contains 0.1-0.2 hundred million CFU/mL of bacillus, 1% of urea, 1% of ammonium sulfate, 2% of calcium superphosphate, 0.1% of ammonium molybdate and 0.1% of borax, and the spraying and fertilizing operations are respectively carried out for 1 time in the initial stage and the later stage;
other growth periods and specific operations are carried out according to the conventional principle in the field, including that after the wheat is matured, a proper node is selected for harvesting, the average SOD content of the wheat seeds is directly measured and calculated, and then the yield per mu is converted. Through measurement and calculation, the average SOD content is 87U/g, and the yield per mu is 570 kg.
Comparative example:
each operation was conducted in accordance with the above examples except that no Bacillus was used in each step, and the average SOD content was 11U/g and the yield per mu was 515kg as measured.

Claims (3)

1. A method for planting wheat rich in SOD comprises applying Bacillus cereus with preservation number of CGMCC NO.0137 to wheat, and applying the Bacillus cereus in a seed pretreatment stage, a flag picking stage and a heading stage; applying the bacillus cereus for 1-2 times in each planting stage; for example, the application of the bacillus cereus is performed 1 time in the seed pretreatment stage and/or the flag picking stage, and repeated 2 times in the heading stage;
the operation of applying the bacillus cereus comprises, in a seed pretreatment phase: the application is achieved by mixing the solution containing the bacillus cereus with the seeds or soaking the seeds (the bacillus concentration can be 1-2 hundred million CFU/mL), and the time for soaking the seeds can be 10-30h, such as 1 day;
a procedure for administering the bacillus cereus, comprising, during the flag stage: spraying a solution containing the Bacillus cereus (Bacillus concentration can be 0.1-0.2 hundred million CFU/mL) on the stem and/or leaf of wheat; a solution containing said bacillus cereus, optionally 1% urea, 1% ammonium sulphate, 2% calcium superphosphate and 0.1% ammonium molybdate;
an operation for applying the bacillus cereus, comprising, during the heading phase: spraying a solution containing the bacillus cereus (bacillus concentration can be 0.1-0.2 hundred million CFU/mL) on the ears, stems and/or leaves of the wheat; the solution containing the bacillus cereus can also contain 1% of urea, 1% of ammonium sulfate, 2% of calcium superphosphate, 0.1% of ammonium molybdate and 0.1% of borax.
2. A method of growing plants according to any preceding claim, wherein the base fertilizer applied before winter sowing comprises per acre: about 15kg of pure nitrogen; phosphorus as P2O5Meter, 5kgLeft and right; k is potassium2About 15kg of O.
3. A method of growing plants according to any preceding claim, wherein, after green return, fertilizers are applied per acre: about 15kg of pure nitrogen; phosphorus as P2O5About 2.5 kg.
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CN110915581A (en) * 2019-11-27 2020-03-27 张家界鱼泉生态农业开发有限公司 Planting method of rice rich in selenium and SOD (superoxide dismutase)
CN115152548A (en) * 2022-07-20 2022-10-11 湖北富民梦农业实业有限公司 SOD (superoxide dismutase) colored wheat and production method thereof

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CN1054398C (en) * 1993-04-21 2000-07-12 北京农业大学 Bacillus capable of producing super oxide dismutase and its production method
CN1807589A (en) * 2006-01-04 2006-07-26 中国农业大学 Method for cultivating superoxide disproportionation enzyme cucumber and its special biological agent
CN102742429A (en) * 2011-04-21 2012-10-24 张艺赢 Superoxide dismutase (SOD) wheat production method
CN106834192A (en) * 2017-03-24 2017-06-13 山东五福生生态工程有限公司 Bacillus cereus solid fermentation method and its tunning and application
CN107593334A (en) * 2017-08-28 2018-01-19 常熟新晟现代农业科技有限公司 A kind of implantation methods of high SOD contents rice

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