CN107593334A - A kind of implantation methods of high SOD contents rice - Google Patents
A kind of implantation methods of high SOD contents rice Download PDFInfo
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Abstract
The invention discloses a kind of implantation methods of high SOD contents rice, comprise the following steps:Seed soaking, vernalization, nursery, rice transplanting, the superoxide dismutase modified using water and lauroyl chloride are configured to the first superoxide dismutase solution seed soaking;Spray water and the superoxide dismutase of lauroyl chloride modification are configured to the second superoxide dismutase solution during vernalization;Bacillus is sprayed during the nursery and water is configured to the first bacillus solution;After rice transplanting, the second bacillus solution is sprayed twice in jointing stage and blooming stage;The 3rd bacillus solution, the preserving number CGMCC No.0175 of bacillus are sprayed in the watery stage.The inventive method, which has, improves disease and insect resistance, effect resistant to lodging, yield increase by more than 10%, and SOD contents increase by more than 10 times compared with general rice in rice.
Description
Technical field
The present invention relates to a kind of paddy rice planting method, more particularly to a kind of implantation methods of high SOD contents rice, belongs to
Proportion of crop planting technical field.
Background technology
Superoxide dismutase Orgotein (Superoxide Dismutase, SOD), alias orgotein, abbreviation:SOD.
SOD is a kind of active material for coming from life entity, can eliminate organism caused harmful substance in metabolic processes.To people
Body, which constantly supplements SOD, has the special-effect of anti-aging.
China Patent No. ZL93103774.3 discloses a kind of bacillus for producing superoxide dismutase and its production
Method, bacillus is selected containing new strains high SOD through mutagenic treatment, strain expands culture, zymocyte liquid, separates, extraction
Technique.Bacterium cell of the present invention is also crushed in addition to through lysozyme and ultrasonic disruption with osmotic pressure, and with 30% and 90% sulfuric acid
Ammonium precipitation, SephadexG-25 desalinations obtain SOD products.Produce the Bacillus numbering of superoxide dismutase:M-22,
Preserving number CGMCC No.0175.
Chinese patent CN104737733A discloses one kind and is rich in SOD milk melons and its production method.Specific method is:
Muskmelon seedses are first soaked seed 30 minutes with 600-800 times of liquid of superoxide dismutase preparation prior to seeding, dry rear seeding and seedling raising;
5 days after muskmelon transplanting, a certain amount of fresh milk is taken, the milk dilution for adding water to convert into 200 times, then by weight addition
0.1%~0.3% superoxide dismutase preparation, milk enzyme compound formulation is configured to, is then uniformly sprayed at sprayer
On the blade and vines of muskmelon, every spray in 15-20 days once, spray 3-5 times altogether.SOD milk sweet teas are rich in using produced by the invention
Melon, which is tasted, has one dense milk fragrant smell, and mouthfeel is extremely unique, and SOD contents significantly improve.But muskmelon is general
Eaten as fruit, SOD supplementary results are limited.
Rice is one kind of draft Oryza, and main most long one kind in Oryza as grain.Rice is again
The important staple food grain in China, but the rice commonly planted can not almost detect SOD activity, and if can increase in rice
SOD contents activity, directly taken in by staple food and supplement SOD, it is highly beneficial to human body, while compared to directly edible SOD
Tonic is more easy to be received by people, and cost is also lower.
Chinese patent CN105961104A has carried out the trial of this respect, and it discloses a kind of kind of high SOD contents rice
Plant method, while also disclose a kind of high SOD contents Rice Cropping fertilizer.The implantation methods of the high SOD contents rice, use
Silicon-calcium-magnesium fertilizer is rice terrace base fertilizer, and rice plant of tillering stage fertilizer is sprayed in rice plant of tillering stage, and promoting Spraying in the flowering stage rice in rice promotees flower
Phase fertilizer, protect Spraying in the flowering stage rice in rice and protect florescence fertilizer, Rice Heading florescence fertilizer is sprayed in Rice Heading florescence,
Grain Filling fertilizer is sprayed in Grain Filling.The implantation methods of high SOD contents rice provided by the invention, it is each according to rice
The growth characteristic in individual growth period, by applying different fertilizer, to promote SOD, CAT, POD, GSH-Px during paddy growth
The synthesis of four kinds of enzymes, and movement is transferred in rice grain, the final rice products for obtaining four kinds of enzymes such as high content SOD.It is led
Multiple amino acid complex is carried out after rice transplanting to rice field and the fertilizer of EDTA complex compounds sprays, by various micro
Element is supplemented to increase the generation of a variety of enzymes such as plant itself SOD.Tillering stage of this method after rice transplanting to rice field, promote
Florescence, full heading time, pustulation period spray respectively heterogeneity proportioning fertilizer, the used various complexity of fertilizer components, no
Implementation easy to operation.
The content of the invention
For above-mentioned the deficiencies in the prior art, it is an object of the invention to provide a kind of implantation methods of high SOD contents rice,
SOD active quantities in rice are improved using simple operation easy to implement.
It is the technical scheme is that such:A kind of implantation methods of high SOD contents rice, comprise the following steps:Leaching
Kind, vernalization, nursery, rice transplanting, the seed soaking are than superoxide dismutase that 7~7.2kg Shui ︰ 1g lauroyl chlorides are modified by weight
The first superoxide dismutase solution is configured to, and it is more molten than 1kg Zhong ︰ the first superoxide dismutases of 2~2.5kg according to weight
Liquid is soaked seed;Weight is configured to second than the superoxide dismutase that 100~200g Shui ︰ 1g lauroyl chlorides are modified during the vernalization
Superoxide dismutase solution, then by weight 1kg Zhong ︰ 25~50g the second superoxide dismutase solution, to the vernalization phase
Seed sprays the second superoxide dismutase solution;Weight is configured to than 50~55g Ya spore Gan Jun ︰ 25kg water during the nursery
First bacillus solution, correspond to Yang Miao ︰ 25kg the first bacillus solution by weight 7~7.5kg seeds and rice shoot is sprayed
First bacillus solution;After the rice transplanting, weight is configured to the second bacillus than 35~40g Ya spore Gan Jun ︰ 25kg water
Solution, 20~22kg the second bacillus solution is sprayed by every mu twice in jointing stage and blooming stage;By weight than 55~60g buds
Spore Gan Jun ︰ 25kg water is configured to the 3rd bacillus solution, and every mu sprays the bacillus solution of 20~22kg the 3rd in the watery stage,
The preserving number CGMCC No.0175 of the bacillus.
Further, it is described vernalization phase seed is sprayed the second superoxide dismutase solution be show money or valuables one carries unintentionally respectively in seed and
The second superoxide dismutase solution is sprayed during 3~5cm of seed sprouting twice, every time by weight 25~50g of 1kg Zhong ︰ the
Two superoxide dismutase spray solutions.
Further, during the first superoxide dismutase solution seed soaking, seed soaking time 40~50 hours, dried in the air after seed soaking
Shine 20~25 hours.
Further, the rice shoot that every mu of rice field plant 7~7.5kg seed is cultivated during the rice transplanting.
Further, the first bacillus solution is sprayed during the nursery when rice shoot length is 8~10cm.
Further, SOD activity in the superoxide dismutase of the lauroyl chloride modification>6000u/mg.
The beneficial effect of technical scheme is sprayed by the seed soaking and vernalization of superoxide dismutase solution,
By seed germ and root absorption superoxide dismutase solution, pass through root, stem, leaf, flower, each portion of fruit with growing process
Metabolism promotes the synthesis of various enzymes, accesses protein building-up process, promotes the SOD from soil to be preserved with protein form
Again in seed.Bacillus solution is sprayed during being additionally incorporated within after nursery, rice transplanting, foliage dressing is carried out, inhales plant
Bacillus solution is received, bacillus enters the content and activity of superoxide dismutase in increase plant in plant.The present invention
The plantation for the rice that method is carried out, have and improve disease and insect resistance, effect resistant to lodging, yield increases by more than 10%, and SOD contains in rice
Amount increases by more than 10 times compared with general rice.
Embodiment
With reference to embodiment, the invention will be further described, but not as a limitation of the invention.
Embodiment 1
The implantation methods of high SOD contents rice, comprise the following steps:Seed soaking, vernalization, nursery, rice transplanting.It is big using southern round-grained rice 46
Rice seed, ratio the first superoxide dismutase of preparation for the superoxide dismutase modified in 7kg water and 1g lauroyl chlorides are molten
Liquid, and according to 1kg Zhong ︰ the first superoxide dismutases of 2kg solution seed soaking 48 hours and dry 24 hours.Seed is put down after drying
It is laid on plastic sheeting and carries out vernalization.When seed shows money or valuables one carries unintentionally, by 140g water and the superoxide dismutase of 1g lauroyl chlorides modification
Ratio prepare the second superoxide dismutase solution.According to 1kg Zhong ︰ 40g the second superoxide dismutase solution to showing money or valuables one carries unintentionally
Seed carry out spray the second superoxide dismutase solution.Prepare the first superoxide dismutase solution and the second superoxides
It is disproportionated SOD activity in the superoxide dismutase of lauroyl chloride modification used in enzyme solutions>6000u/mg.
Seed after vernalization is moved into seedbed and carries out nursery, the ratio that 25kg water is closed in 55g bacillus prepares the first bud
Spore bacillus solution.Yang Miao ︰ 25kg the first bacillus solution is corresponded to seedling according to 7kg seeds when rice shoot average length is 10cm
Seedling sprays the first bacillus solution;After shoot transplanting equipment is entered rice field by the rice shoot cultivated with every mu of rice field plant 7kg seed, point
The second bacillus solution and 55g bacillus and 25kg water are not prepared according to the ratio of 35g bacillus and 25kg water
Ratio prepares the 3rd bacillus solution.20kg the second bacillus solution is sprayed in jointing stage and blooming stage by every mu twice;
Every mu sprays the bacillus solution of 20kg the 3rd in the watery stage.Prepare the first bacillus solution, the second bacillus solution and
The preserving number CGMCC No.0175 of bacillus used by 3rd bacillus solution.
Embodiment 2
The implantation methods of high SOD contents rice, comprise the following steps:Seed soaking, vernalization, nursery, rice transplanting.It is big using southern round-grained rice 46
Rice seed, the ratio for the superoxide dismutase modified in 7.2kg water and 1g lauroyl chlorides prepare the first superoxide dismutase
Solution, and according to 1kg Zhong ︰ the first superoxide dismutases of 2.2kg solution seed soaking 40 hours and dry 20 hours.Planted after drying
Son, which is laid on plastic sheeting, carries out vernalization.When seed shows money or valuables one carries unintentionally, by 100g water and the superoxides discrimination of 1g lauroyl chlorides modification
The ratio for changing enzyme prepares the second superoxide dismutase solution.According to 1kg Zhong ︰ 25g the second superoxide dismutase solution pair
The seed to show money or valuables one carries unintentionally carries out spraying the second superoxide dismutase solution.Prepare the first superoxide dismutase solution and the second super oxygen
SOD activity in the superoxide dismutase of lauroyl chloride modification used in compound disproportionation enzyme solutions>6000u/mg.
Seed after vernalization is moved into seedbed and carries out nursery, the ratio that 25kg water is closed in 50g bacillus prepares the first bud
Spore bacillus solution.When rice shoot average length is 8cm Yang Miao ︰ 25kg the first bacillus solution pair is corresponded to according to 7.2kg seeds
Rice shoot sprays the first bacillus solution;Shoot transplanting equipment is entered by rice field with the rice shoot that every mu of rice field plant 7.2kg seed is cultivated
Afterwards, respectively according to the ratio of 38g bacillus and 25kg water prepare the second bacillus solution and 57.5g bacillus and
The ratio of 25kg water prepares the 3rd bacillus solution.22kg the second gemma bars are sprayed in jointing stage and blooming stage by every mu twice
Bacterium solution;Every mu sprays the bacillus solution of 20kg the 3rd in the watery stage.Prepare the first bacillus solution, the second bacillus
The preserving number CGMCC No.0175 of bacillus used by solution and the 3rd bacillus solution.
Embodiment 3
The implantation methods of high SOD contents rice, comprise the following steps:Seed soaking, vernalization, nursery, rice transplanting.It is big using southern round-grained rice 46
Rice seed, ratio the first superoxide dismutase of preparation for the superoxide dismutase modified in 7kg water and 1g lauroyl chlorides are molten
Liquid, and according to 1kg Zhong ︰ the first superoxide dismutases of 2.5kg solution seed soaking 50 hours and dry 25 hours.Seed after drying
It is laid on plastic sheeting and carries out vernalization.When seed shows money or valuables one carries unintentionally, by 200g water and the superoxide dismutase of 1g lauroyl chlorides modification
The ratio of enzyme prepares the second superoxide dismutase solution.According to 1kg Zhong ︰ 50g the second superoxide dismutase solution to dew
White seed carries out spraying the second superoxide dismutase solution.Prepare the first superoxide dismutase solution and the second super oxygen
SOD activity in the superoxide dismutase of lauroyl chloride modification used in thing disproportionation enzyme solutions>6000u/mg.
Seed after vernalization is moved into seedbed and carries out nursery, the ratio that 25kg water is closed in 57.5g bacillus prepares first
Bacillus solution.When rice shoot average length is 9cm Yang Miao ︰ 25kg the first bacillus solution pair is corresponded to according to 7kg seeds
Rice shoot sprays the first bacillus solution;After shoot transplanting equipment is entered rice field by the rice shoot cultivated with every mu of rice field plant 7kg seed,
Respectively the second bacillus solution and 60g bacillus and 25kg water are prepared according to the ratio of 40g bacillus and 25kg water
Ratio prepare the 3rd bacillus solution.It is molten that the bacillus of 20kg second is sprayed in jointing stage and blooming stage by every mu twice
Liquid;Every mu sprays the bacillus solution of 22kg the 3rd in the watery stage.Prepare the first bacillus solution, the second bacillus solution
With the preserving number CGMCC No.0175 of bacillus used by the 3rd bacillus solution.
Embodiment 4
The implantation methods of high SOD contents rice, comprise the following steps:Seed soaking, vernalization, nursery, rice transplanting.It is big using southern round-grained rice 46
Rice seed, ratio the first superoxide dismutase of preparation for the superoxide dismutase modified in 7kg water and 1g lauroyl chlorides are molten
Liquid, and according to 1kg Zhong ︰ the first superoxide dismutases of 2kg solution seed soaking 48 hours and dry 24 hours.Seed is put down after drying
It is laid on plastic sheeting and carries out vernalization.When seed shows money or valuables one carries unintentionally, by 140g water and the superoxide dismutase of 1g lauroyl chlorides modification
Ratio prepare the second superoxide dismutase solution.According to 1kg Zhong ︰ 40g the second superoxide dismutase solution to showing money or valuables one carries unintentionally
Seed carry out spray the second superoxide dismutase solution.And in seed sprouting 3.5cm according to 1kg Zhong ︰ 40g second
Superoxide dismutase solution carries out spraying the second superoxide dismutase solution to the seed of emergence.Prepare the first superoxides
SOD in the superoxide dismutase of lauroyl chloride modification used in enzyme solutions and the second superoxide dismutase solution is disproportionated to live
Property>6000u/mg.
Seed after vernalization is moved into seedbed and carries out nursery, the ratio that 25kg water is closed in 55g bacillus prepares the first bud
Spore bacillus solution.Yang Miao ︰ 25kg the first bacillus solution is corresponded to seedling according to 7kg seeds when rice shoot average length is 10cm
Seedling sprays the first bacillus solution;After shoot transplanting equipment is entered rice field by the rice shoot cultivated with every mu of rice field plant 7kg seed, point
The second bacillus solution and 55g bacillus and 25kg water are not prepared according to the ratio of 35g bacillus and 25kg water
Ratio prepares the 3rd bacillus solution.20kg the second bacillus solution is sprayed in jointing stage and blooming stage by every mu twice;
Every mu sprays the bacillus solution of 20kg the 3rd in the watery stage.Prepare the first bacillus solution, the second bacillus solution and
The preserving number CGMCC No.0175 of bacillus used by 3rd bacillus solution.
Embodiment 5
The implantation methods of high SOD contents rice, comprise the following steps:Seed soaking, vernalization, nursery, rice transplanting.It is big using southern round-grained rice 46
Rice seed, the ratio for the superoxide dismutase modified in 7.2kg water and 1g lauroyl chlorides prepare the first superoxide dismutase
Solution, and according to 1kg Zhong ︰ the first superoxide dismutases of 2.2kg solution seed soaking 40 hours and dry 20 hours.Planted after drying
Son, which is laid on plastic sheeting, carries out vernalization.When seed shows money or valuables one carries unintentionally, by 100g water and the superoxides discrimination of 1g lauroyl chlorides modification
The ratio for changing enzyme prepares the second superoxide dismutase solution.According to 1kg Zhong ︰ 25g the second superoxide dismutase solution pair
The seed to show money or valuables one carries unintentionally carries out spraying the second superoxide dismutase solution.And in seed sprouting 4.5cm according to 1kg Zhong ︰ 40g
Second superoxide dismutase solution carries out spraying the second superoxide dismutase solution to the seed of emergence.Prepare the first super oxygen
Compound is disproportionated in the superoxide dismutase of lauroyl chloride modification used in enzyme solutions and the second superoxide dismutase solution
SOD activity>6000u/mg.
Seed after vernalization is moved into seedbed and carries out nursery, the ratio that 25kg water is closed in 50g bacillus prepares the first bud
Spore bacillus solution.When rice shoot average length is 8cm Yang Miao ︰ 25kg the first bacillus solution pair is corresponded to according to 7.2kg seeds
Rice shoot sprays the first bacillus solution;Shoot transplanting equipment is entered by rice field with the rice shoot that every mu of rice field plant 7.2kg seed is cultivated
Afterwards, respectively according to the ratio of 38g bacillus and 25kg water prepare the second bacillus solution and 57.5g bacillus and
The ratio of 25kg water prepares the 3rd bacillus solution.22kg the second gemma bars are sprayed in jointing stage and blooming stage by every mu twice
Bacterium solution;Every mu sprays the bacillus solution of 20kg the 3rd in the watery stage.Prepare the first bacillus solution, the second bacillus
The preserving number CGMCC No.0175 of bacillus used by solution and the 3rd bacillus solution.
Comparative example 1
The implantation methods of rice, comprise the following steps:Seed soaking, vernalization, nursery, rice transplanting.Using the southern rice seed of round-grained rice 46, press
Ratio the first superoxide dismutase solution of preparation of 7kg water and the superoxide dismutase of 1g lauroyl chlorides modification, and according to
1kg Zhong ︰ the first superoxide dismutases of 2kg solution seed soaking 48 hours simultaneously dries 24 hours.Prepare the first superoxide dismutase
SOD activity in the superoxide dismutase that lauroyl chloride used in enzyme solutions is modified>6000u/mg.Remaining step is compared with skill
Art.
Comparative example 2
The implantation methods of rice, comprise the following steps:Seed soaking, vernalization, nursery, rice transplanting.Only when seed shows money or valuables one carries unintentionally, by 140g
The ratio of water and the superoxide dismutase of 1g lauroyl chlorides modification prepares the second superoxide dismutase solution.According to 1kg kinds
Sub- ︰ 40g the second superoxide dismutase solution carries out spraying the second superoxide dismutase solution to the seed to show money or valuables one carries unintentionally.Prepare the
SOD activity in the superoxide dismutase that lauroyl chloride used in two superoxide dismutase solution is modified>6000u/mg.Its
Remaining step is compared with technology.
Comparative example 3
The implantation methods of rice, comprise the following steps:Seed soaking, vernalization, nursery, rice transplanting., will using the southern rice seed of round-grained rice 46
Seed after vernalization moves into seedbed and carries out nursery, and the ratio that 25kg water is closed in 55g bacillus prepares the first bacillus solution.
Yang Miao ︰ 25kg the first bacillus solution is corresponded to rice shoot sprinkling first according to 7kg seeds when rice shoot average length is 10cm
Bacillus solution;After shoot transplanting equipment is entered rice field by the rice shoot cultivated with every mu of rice field plant 7kg seed, respectively according to 35g
The ratio of bacillus and 25kg water prepares the second bacillus solution and the ratio of 55g bacillus and 25kg water and prepares the
Three bacillus solution.20kg the second bacillus solution is sprayed in jointing stage and blooming stage by every mu twice;It is every in the watery stage
Mu sprays the bacillus solution of 20kg the 3rd.Prepare the first bacillus solution, the second bacillus solution and the 3rd gemma bar
The preserving number CGMCC No.0175 of bacillus used by bacterium solution.Remaining step is compared with technology.
Comparative example 4
Used seed is southern round-grained rice 505, and specific implementation step is the same as embodiment 1.
Comparative example 5
Used seed is southern round-grained rice 505, and specific implementation step is the same as embodiment 4.
The yield of the various embodiments described above and comparative example is counted, and each embodiment, comparative example and commercially available Northeastern S OD rice are pressed
SOD active quantities are detected according to national standard GB/T5009.171.2003, as a result such as following table.
From upper table result, pass through being used respectively to seed soaking, vernalization, nursery, rice transplanting different times joint for the present invention
The superoxide dismutase solution and bacillus solution of the superoxide dismutase configuration of lauroyl chloride modification can greatly improve
SOD active quantities in rice yield and raising rice, more single progress superoxide dismutase solution seed soaking, vernalization or use
Bacillus spray solution rice shoot, plant have larger yield and SOD active quantities to improve.
Claims (6)
1. a kind of implantation methods of high SOD contents rice, comprise the following steps:Seed soaking, vernalization, nursery, rice transplanting, its feature exist
In the seed soaking is that weight is configured into the first super oxygen than the superoxide dismutase that 7~7.2kg Shui ︰ 1g lauroyl chlorides are modified
Compound is disproportionated enzyme solutions, and according to weight ︰ 2~2.5kg than 1kg kind the first superoxide dismutase solution seed soakings;It is described to urge
Weight is configured to the second superoxide dismutase than the superoxide dismutase that 100~200g Shui ︰ 1g lauroyl chlorides are modified during bud
Enzyme solutions, then by weight 1kg Zhong ︰ 25~50g the second superoxide dismutase solution, to vernalization phase seed sprinkling second
Superoxide dismutase solution;Weight is configured to the first bacillus than 50~55g Ya spore Gan Jun ︰ 25kg water during the nursery
Solution, correspond to Yang Miao ︰ 25kg the first bacillus solution by weight 7~7.5kg seeds and the first bacillus is sprayed to rice shoot
Solution;After the rice transplanting, weight is configured to the second bacillus solution than 35~40g Ya spore Gan Jun ︰ 25kg water, in the jointing stage
20~22kg the second bacillus solution is sprayed with blooming stage by every mu twice;By weight than 55~60g Ya spore Gan Jun ︰ 25kg water
The 3rd bacillus solution is configured to, every mu sprays the bacillus solution of 20~22kg the 3rd, the bacillus in the watery stage
Preserving number CGMCC No.0175.
2. the implantation methods of high SOD contents rice according to claim 1, it is characterised in that described to vernalization phase seed
It is to spray the second super oxygen twice when seed shows money or valuables one carries unintentionally with 3~5cm of seed sprouting respectively to spray the second superoxide dismutase solution
Compound is disproportionated enzyme solutions, every time by weight sub- the second superoxide dismutases of 25~50g of the ︰ spray solution of 1kg kinds.
3. the implantation methods of high SOD contents rice according to claim 1, it is characterised in that first superoxides
During mutase solution seed soaking, seed soaking time 40~50 hours, dried 20~25 hours after seed soaking.
4. the implantation methods of high SOD contents rice according to claim 1, it is characterised in that every mu of rice during the rice transplanting
The rice shoot that field plant 7~7.5kg seeds are cultivated.
5. the implantation methods of high SOD contents rice according to claim 1, it is characterised in that in rice shoot during the nursery
Length sprays the first bacillus solution when being 8~10cm.
6. the implantation methods of high SOD contents rice according to claim 1, it is characterised in that the lauroyl chloride modification
Superoxide dismutase in SOD activity>6000u/mg.
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CN113331009B (en) * | 2018-01-30 | 2021-12-03 | 五月阳光生物科技(浙江)有限公司 | Planting method of rice with high trace elements and high SOD (superoxide dismutase) |
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CN110012819A (en) * | 2019-04-03 | 2019-07-16 | 孙彦升 | A kind of fruits and vegetables and its yield-increasing planting technique and application rich in SOD |
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CN110100668A (en) * | 2019-06-17 | 2019-08-09 | 郑州市农林科学研究所 | The implantation methods of cereal crop rich in SOD |
CN110342979A (en) * | 2019-07-11 | 2019-10-18 | 中国农业科学院作物科学研究所 | A kind of rice fertilizer synergist and preparation method thereof and application method |
CN114831235A (en) * | 2022-06-01 | 2022-08-02 | 五月阳光生物科技(浙江)有限公司 | Preparation process of SOD (superoxide dismutase) stock solution |
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