CN112979391B - Root-promoting bio-organic fertilizer and preparation method thereof - Google Patents
Root-promoting bio-organic fertilizer and preparation method thereof Download PDFInfo
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- CN112979391B CN112979391B CN202110389549.4A CN202110389549A CN112979391B CN 112979391 B CN112979391 B CN 112979391B CN 202110389549 A CN202110389549 A CN 202110389549A CN 112979391 B CN112979391 B CN 112979391B
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/02—Other organic fertilisers from peat, brown coal, and similar vegetable deposits
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
Abstract
The invention relates to a root-promoting bio-organic fertilizer which comprises a compound microorganism and humate, wherein the compound organism comprises Paenibacillus polymyxa (Paenibacillus polymyxa), Streptomyces microflavus (Streptomyces microflavus), Trichoderma asperellum (Trichoderma asperellum) and Azotobacter chroococcum (Azotobacter chroococcum). The biological organic fertilizer can enhance the activity of plant roots. The invention also relates to a preparation method and application of the biological organic fertilizer.
Description
Technical Field
The invention belongs to the field of special functional fertilizers for agriculture, and particularly relates to a root-promoting bio-organic fertilizer and a preparation method thereof.
Background
The microbial agent refers to a preparation containing a living body of a specific microorganism, which increases the supply of nutrients to plants or promotes the growth of plants, improves the growth of plants, and the like, by the action of the microorganism. Compared with chemical fertilizers, the microbial fertilizer serving as a biological agent has the advantages of being ecological, safe, harmless, free of environmental pollution, free of damage to soil structures, durable in effect, capable of improving yield and quality and the like.
Chinese patent application CN201610717360.2 discloses a seedling homogenizing and stem strengthening agent, which comprises an organic fertilizer, an inorganic fertilizer, a carrier, composite bacteria powder and the like, wherein the composite bacteria powder comprises bacillus amyloliquefaciens, bacillus subtilis, bacillus mucilaginosus, paenibacillus polymyxa, bacillus laterosporus, streptomyces microflavus and streptomyces globisporus. The preparation can homogenize seedling, strengthen stalk, strengthen root, raise corn yield, etc.
CN201910180390.8 discloses a microbial fertilizer, which comprises the following components: 1-3% of microbial agent, 80-90% of carrier and the balance of water. The microbial agent comprises one or more of bacillus subtilis, bacillus amyloliquefaciens, bacillus mucilaginosus, bacillus laterosporus, bacillus licheniformis and bacillus megaterium.
CN201480016765.2 discloses a synergistic composition comprising a bacillus subtilis strain and a biopesticide, which can improve plant health, e.g. improve drought resistance; moreover, crop yield can also be increased.
CN201310175512.7 discloses a rooting agent with disease-resistant and growth-promoting effects for transplanting tobacco crops, the formula of which comprises a compound microbial agent, fulvic acids and alginic acids. The compound microbial agent is streptomycete and trichoderma longibrachiatum.
At present, microbial agents on the market are various in types, but are serious in homogenization phenomenon, different in efficacy and lack of pertinence.
Summary of The Invention
The invention aims to develop a root-promoting bio-organic fertilizer, which can obviously increase the activity of a plant root system through the synergistic action of microbial thalli after being applied to crops. The invention is realized by the following technical scheme.
The invention relates to a biological organic fertilizer for enhancing plant root activity, which comprises the following components in parts by weight:
10-40 parts of compound microorganism;
60-90 parts of humate;
wherein the complex organism comprises Paenibacillus polymyxa (Paenibacillus polymyxa), Streptomyces microflavus (Streptomyces microflavus), Trichoderma asperellum (Trichoderma asperellum).
In a preferred embodiment of the present invention, the weight parts of each component are preferably:
15-30 parts of compound microorganism;
70-85 parts of humate.
In a preferred embodiment of the present invention, wherein the weight ratio of Paenibacillus polymyxa (Paenibacillus polymyxa), Streptomyces microflavus (Streptomyces microflavus) and Trichoderma asperellum (Trichoderma asperellum) in the composite microorganism is 1: 0.5-5: 0.2-2.
In a preferred embodiment of the present invention, wherein the complex microorganism further comprises Azotobacter chroococcum (Azotobacter chroococcum).
In a preferred embodiment of the present invention, wherein the weight ratio of Paenibacillus polymyxa (Paenibacillus polymyxa), Streptomyces microflavus (Streptomyces microflavus), Trichoderma asperellum (Trichoderma asperellum), and Azotobacter chroococci (Azotobacter chroococcum) in the composite microorganism is 1: 0.5-5: 0.2-2: 0.8-2.4.
In a preferred embodiment of the present invention, wherein the humate is selected from one or two of potassium humate and sodium humate.
In a preferred embodiment of the present invention, wherein the humate is selected from potassium humate.
The invention also relates to a preparation method of the biological organic fertilizer, which comprises the following steps:
(1) preparation of microbial cells:
separately performing slant culture, seed culture and fermentation culture on Paenibacillus polymyxa (Paenibacillus polymyxa), Streptomyces microflavus (Streptomyces microflavus), Trichoderma asperellum and Azotobacter chroococcum to obtain fermentation cultures; then, each microbial thallus is obtained through post-treatment;
(2) preparing a biological organic fertilizer:
uniformly mixing the microbial thallus obtained in the formula amount step (1) with the humate in the formula amount to obtain the bio-organic fertilizer.
The invention also relates to application of the biological organic fertilizer in enhancing the activity of plant roots.
In a preferred embodiment of the present invention, wherein the plant is tomato.
Detailed description of the invention:
the invention relates to a biological organic fertilizer for enhancing plant root activity, which comprises the following components in parts by weight:
10-40 parts of compound microorganism;
60-90 parts of humate;
in a preferred embodiment of the invention, the content of each component is preferably 15-30 parts of compound microorganism, 70-85 parts of humate; more preferably 20-25 parts of compound microorganism and 75-80 parts of humate.
In the invention, the complex organism comprises Paenibacillus polymyxa (Paenibacillus polymyxa), Streptomyces microflavus (Streptomyces microflavus) and Trichoderma asperellum (Trichoderma asperellum), and the weight ratio of the microorganisms is 1: 0.5-5: 0.2-2, preferably 1: 1-4: 0.4-1.2, and more preferably 1: 2-3: 0.6-0.8.
In a preferred embodiment of the present invention, wherein the complex microorganism further comprises Azotobacter chroococcum (Azotobacter chroococcum). Wherein the weight ratio of the Paenibacillus polymyxa (Paenibacillus polymyxa), the Streptomyces microflavus (Streptomyces microflavus), the Trichoderma asperellum (Trichoderma asperellum) and the Azotobacter chroococcum (Azotobacter chroococcum) is 1: 0.5-5: 0.2-2: 0.8-2.4, preferably 1: 1-4: 0.4-1.2: 1.4-2.1, more preferably 1: 2-3: 0.6-0.8: 1.6-1.8.
The above microbial species are commercially available. For example, the bacillus polymyxa (Paenibacillus polymyxa) is available from China agricultural microorganism culture collection and management center, and the accession number of the bacillus polymyxa is ACCC 10750; the Streptomyces microflavus has a deposit number of ACCC 40776; the Trichoderma asperellum (Trichoderma asperellum) is deposited with ACCC 31650; the Azotobacter chroococcum (Azotobacter chroococcum) is deposited under the accession number ACCC 10099.
The preparation method of the microbial thallus comprises the steps of respectively subjecting the obtained Paenibacillus polymyxa, Streptomyces microflavus, Trichoderma asperellum and Azotobacter chroococcum to slant culture, seed culture and fermentation culture to obtain fermentation cultures; then, each microbial cell is obtained through post-treatment. The obtained microbial cells have an effective viable count of 2 × 1010-8×1010cfu/g; the effective viable count of Streptomyces microflavus is 3.2 × 107-7.5×107cfu/g; the effective viable count of Trichoderma asperellum (Trichoderma asperellum) is 5.6 × 108-8×108cfu/g; the effective viable count of Azotobacter chroococcum is 2.7 × 108-6×108cfu/g。
The invention also relates to a preparation method of the biological organic fertilizer, which comprises the following steps:
(1) preparation of microbial cells:
separately performing slant culture, seed culture and fermentation culture on Paenibacillus polymyxa (Paenibacillus polymyxa), Streptomyces microflavus (Streptomyces microflavus), Trichoderma asperellum and Azotobacter chroococcum to obtain fermentation cultures; then, each microbial thallus is obtained through post-treatment;
(2) preparing a biological organic fertilizer:
and (2) uniformly mixing the microbial thallus obtained in the step (1) with the humate in a formula amount to obtain the bio-organic fertilizer.
Wherein:
in the preparation of the microbial cells in step (1), the preparation steps of each microbial cell are as follows:
preparation of Paenibacillus polymyxa (Paenibacillus polymyxa) thallus:
A. slant culture: inoculating the strain on slant culture medium, and culturing at 33-38 deg.C for 20-35 h. The slant culture medium is as follows: 3g/L beef extract, 10g/L peptone, 5g/L sodium chloride and 15-20g/L agar, and is prepared by using distilled water.
B. Seed cultivation by shaking table: inoculating the slant culture strain in seed culture medium at an inoculum size of 5-10% (volume fraction), and culturing at 30-35 deg.C under 150-. The seed culture medium is as follows: 3g/L beef extract, 10g/L peptone and 5g/L sodium chloride, and is prepared by using distilled water.
C. Fermentation culture: inoculating 10-15% of strain cultured by shaking table seed into fermentation culture medium, and fermenting at 33-37 deg.C and rotation speed of 200-. The fermentation medium is as follows: 50g/L glucose, 10g/L peptone, 5g/L yeast extract, 0.8g/L magnesium sulfate, 1.2g/L ammonium sulfate, and 1g/L potassium dihydrogen phosphate, and is prepared from distilled water.
D. Preparing a microbial thallus: centrifuging the fermentation culture at 3000-5000rpm for 10-30min, collecting the precipitate, and spray drying to obtain microbial thallus.
Preparation of Streptomyces microflavus (Streptomyces microflavus) thallus:
A. slant culture: inoculating the strain on slant culture medium, and culturing at 25-32 deg.C for 48-72 h. The slant culture medium is as follows: 3g/L beef extract, 10g/L peptone, 5g/L sodium chloride and 15-20g/L agar, and is prepared by using distilled water.
B. Seed cultivation by shaking table: inoculating the slant culture strain in seed culture medium at an inoculum size of 5-10% (volume fraction), and culturing at 28-33 deg.C under 150-. The seed culture medium is as follows: 3g/L beef extract, 10g/L peptone and 5g/L sodium chloride, and is prepared by using distilled water.
C. Fermentation culture: inoculating 10-15% of strain cultured by shaking table seed into fermentation culture medium, and fermenting at 33-37 deg.C and rotation speed of 200-. The fermentation medium is as follows: 50g/L glucose, 10g/L peptone, 5g/L yeast extract, 0.8g/L magnesium sulfate, 1g/L potassium dihydrogen phosphate and 0.5g/L sodium carbonate, and is prepared by using distilled water.
D. Preparing a microbial thallus: centrifuging the fermentation culture at 3000-5000rpm for 10-30min, collecting the precipitate, and spray drying to obtain microbial thallus.
Preparation of Trichoderma asperellum (Trichoderma asperellum) cells:
A. slant culture: inoculating the strain on slant culture medium, and culturing at 25-28 deg.C for 3-5 days. The slant culture medium is a PDA culture medium: 200g/L of potato, 20g/L of glucose and 18g/L of agar are prepared by distilled water.
B. Seed cultivation by shaking table: the slant culture strain is inoculated in seed culture medium under aseptic condition, and cultured at 25-28 deg.C and 180-240rpm for 3-5 d. The seed culture medium is a PD culture medium: 200g/L of potato and 20g/L of glucose, and is prepared by distilled water.
C. Fermentation culture: inoculating the strain cultured by seed in 1-10% (volume fraction) into fermentation culture medium, and fermenting at 25-30 deg.C for 5-6 days to obtain fermentation culture. The fermentation medium is as follows: 50g/L corn flour, 10g/L cane sugar, 0.5g/L magnesium sulfate, 1.8g/L ammonium sulfate, 3.6g/L monopotassium phosphate, 0.005g/L ferrous sulfate heptahydrate, 0.92g/L sodium nitrate, 0.75g/L sodium chloride and 0.008g/L zinc sulfate.
D. Preparing a microbial thallus: and (3) centrifuging the fermentation culture at 4000-6000rpm for 15-30min, collecting the precipitate, and spray drying to obtain the microbial thallus.
Preparation of Azotobacter chroococcum (Azotobacter chroococcum) thallus:
A. slant culture: inoculating the strain on slant culture medium, and culturing at 25-28 deg.C for 48-72 h. The slant culture medium is as follows: 10g/L glucose, 0.2g/L dipotassium hydrogen phosphate, 0.2g/L potassium sulfate, 0.2g/L sodium chloride, 5g/L calcium carbonate, 0.2g/L magnesium sulfate and 18g/L agar, and preparing by using distilled water.
B. Seed cultivation by shaking table: inoculating the slant culture strain in seed culture medium at an inoculum size of 5-10% (volume fraction), and culturing at 26-32 deg.C under 140-190rpm for 48-72 h. The seed culture medium is as follows: 10g/L glucose, 0.2g/L dipotassium hydrogen phosphate, 0.2g/L potassium sulfate, 0.2g/L sodium chloride, 5g/L calcium carbonate, 0.2g/L magnesium sulfate and distilled water.
C. Fermentation culture: inoculating 2-10% of strain cultured by shaking table seed into fermentation culture medium, and fermenting at 28-32 deg.C and 200-220rpm for 3-5d to obtain fermentation culture. The fermentation medium is as follows: 10g/L of sucrose, 5g/L of yeast extract, 0.5g/L of magnesium sulfate, 1g/L of monopotassium phosphate, 0.2g/L of sodium carbonate and 0.01g/L of ferrous sulfate, and is prepared by using distilled water.
D. Preparing a microbial thallus: centrifuging the fermentation culture at 3000-5000rpm for 10-30min, collecting the precipitate, and spray drying to obtain microbial thallus.
In the preparation process of the biological organic fertilizer in the step (2), various microbial thalli obtained in the step (1) can be directly mixed according to the formula amount, and then the microbial thalli mixture and the humate according to the formula amount are uniformly mixed to obtain the biological organic fertilizer. Preferably, the microbial thalli and a proper amount of humate are mixed to obtain a mixture of single microbial thalli and humate, and then the mixture of the microbial thalli and humic acid is mixed with the balance of humate in the formula to obtain the biological organic fertilizer.
In the invention, the humate is selected from one or two of potassium humate and sodium humate, for example, potassium humate, sodium humate or a mixture of potassium humate and sodium humate is selected. Preferably, the humate is selected from potassium humate.
The invention also relates to application of the biological organic fertilizer in enhancing the activity of plant roots. When used, it is applied to the roots of plants, and the specific application method is not particularly limited, and for example, it may be broadcast application, hole application, watered spray application, irrigation application, and the like. The application amount is also not particularly limited and may be adjusted depending on the crops, and is usually, for example, 2 to 20 kg/mu, preferably 5 to 15 kg/mu. The biological organic fertilizer can be used for various plants, such as field crops, fruit trees, vegetables and the like, so as to enhance the activity of plant root systems. The preferred crop for application is tomato.
The biological organic fertilizer can obviously increase the activity of the root system of the plant by the mixed application of the humate and the microbial thallus and the combined synergistic action of a plurality of microbes, which is beneficial to the growth of the plant.
Detailed Description
The invention is further illustrated by the following examples for the understanding of the invention, but is not limited thereto.
Preparation of microbial thallus
The microorganisms used in the embodiment of the invention are all purchased from China agricultural microbial culture collection management center, wherein the preservation number of Paenibacillus polymyxa (Paenibacillus polymyxa) is ACCC 10750; the Streptomyces microflavus has a deposit number of ACCC 40776; the Trichoderma asperellum (Trichoderma asperellum) is deposited with ACCC 31650; the Azotobacter chroococcum (Azotobacter chroococcum) is deposited under the accession number ACCC 10099.
(1) Preparation of Paenibacillus polymyxa (Paenibacillus polymyxa) ACCC10750 cells:
A. slant culture: inoculating the strain on slant culture medium, and culturing at 35 deg.C for 30 h. The slant culture medium is as follows: 3g/L beef extract, 10g/L peptone, 5g/L sodium chloride and 18g/L agar, and is prepared by using distilled water.
B. Seed cultivation by shaking table: the slant culture of the strain was inoculated in 5% (volume fraction) of the seed medium, and cultured at 35 ℃ for 24 hours at 150 rpm. The seed culture medium is as follows: 3g/L beef extract, 10g/L peptone and 5g/L sodium chloride, and is prepared by using distilled water.
C. Fermentation culture: inoculating the strain cultured by the shaking table seed into a fermentation culture medium with the inoculation amount of 10%, and fermenting and culturing for 48h at the temperature of 35 ℃ and the rotation speed of 200rpm to obtain a fermentation culture. The fermentation medium is as follows: 50g/L glucose, 10g/L peptone, 5g/L yeast extract, 0.8g/L magnesium sulfate, 1.2g/L ammonium sulfate, and 1g/L potassium dihydrogen phosphate, and is prepared from distilled water.
D. Preparing a microbial thallus: centrifuging the fermentation culture at 4000rpm for 30min, collecting precipitate, spray drying to obtain microbial thallus, and measuring effective viable count to be 4.8 × 1010cfu/g。
(2) Preparation of Streptomyces microflavus (Streptomyces microflavus) ACCC 40776 bacteria:
A. slant culture: the strain is inoculated on a slant culture medium and cultured for 72h at the temperature of 28 ℃. The slant culture medium is as follows: 3g/L beef extract, 10g/L peptone, 5g/L sodium chloride and 18g/L agar, and is prepared by using distilled water.
B. Seed cultivation by shaking table: the slant culture of the strain was inoculated in 5% (volume fraction) of the seed medium, and cultured at 30 ℃ for 48 hours at 200 rpm. The seed culture medium is as follows: 3g/L beef extract, 10g/L peptone and 5g/L sodium chloride, and is prepared by using distilled water.
C. Fermentation culture: inoculating the strain cultured by the shaking table seed into a fermentation culture medium with the inoculation amount of 10%, and fermenting and culturing for 48h at the temperature of 35 ℃ and the rotating speed of 220rpm to obtain a fermentation culture. The fermentation medium is as follows: 50g/L glucose, 10g/L peptone, 5g/L yeast extract, 0.8g/L magnesium sulfate, 1g/L potassium dihydrogen phosphate and 0.5g/L sodium carbonate, and is prepared by using distilled water.
D. Preparing a microbial thallus: centrifuging the fermentation culture at 4000rpm for 20min, collecting precipitate, spray drying to obtain microbial thallus, and measuringThe effective viable count is 6.5 multiplied by 107cfu/g。
(3) Preparation of Trichoderma asperellum (Trichoderma asperellum) ACCC31650 cells:
A. slant culture: the strain was inoculated on a slant medium and cultured at 28 ℃ for 4 days. The slant culture medium is a PDA culture medium: 200g/L of potato, 20g/L of glucose and 18g/L of agar are prepared by distilled water.
B. Seed cultivation by shaking table: the slant culture strain was inoculated into seed medium under aseptic conditions and cultured at 28 ℃ and 180rpm for 5 days. The seed culture medium is a PD culture medium: 200g/L of potato and 20g/L of glucose, and is prepared by distilled water.
C. Fermentation culture: inoculating the strain cultured by the seeds into a fermentation culture medium in an inoculation amount of 5 percent (volume fraction), and fermenting and culturing for 6 days at the temperature of 30 ℃ to obtain a fermentation culture. The fermentation medium is as follows: 50g/L corn flour, 10g/L cane sugar, 0.5g/L magnesium sulfate, 1.8g/L ammonium sulfate, 3.6g/L monopotassium phosphate, 0.005g/L ferrous sulfate heptahydrate, 0.92g/L sodium nitrate, 0.75g/L sodium chloride and 0.008g/L zinc sulfate.
D. Preparing a microbial thallus: centrifuging the fermentation culture at 4000rpm for 15min, collecting precipitate, spray drying to obtain microbial thallus, and determining effective viable count of 7.8 × 108cfu/g。
(4) Preparation of Azotobacter chroococcum ACCC 10099 bacteria:
A. slant culture: the strain is inoculated on a slant culture medium and cultured for 72h at the temperature of 26 ℃. The slant culture medium is as follows: 10g/L glucose, 0.2g/L dipotassium hydrogen phosphate, 0.2g/L potassium sulfate, 0.2g/L sodium chloride, 5g/L calcium carbonate, 0.2g/L magnesium sulfate and 18g/L agar, and preparing by using distilled water.
B. Seed cultivation by shaking table: the slant culture of the strain was inoculated in 5% (volume fraction) of the seed medium, and cultured at 26 ℃ for 72 hours at 150 rpm. The seed culture medium is as follows: 10g/L glucose, 0.2g/L dipotassium hydrogen phosphate, 0.2g/L potassium sulfate, 0.2g/L sodium chloride, 5g/L calcium carbonate, 0.2g/L magnesium sulfate and distilled water.
C. Fermentation culture: inoculating the strain cultured by shaking table seed in 5% of inoculum size in fermentation culture medium, fermenting and culturing at 30 deg.C and rotation speed of 200rpm for 4d to obtain fermentation culture. The fermentation medium is as follows: 10g/L of sucrose, 5g/L of yeast extract, 0.5g/L of magnesium sulfate, 1g/L of monopotassium phosphate, 0.2g/L of sodium carbonate and 0.01g/L of ferrous sulfate, and is prepared by using distilled water.
D. Preparing a microbial thallus: centrifuging the fermentation culture at 3000rpm for 25min, collecting precipitate, spray drying to obtain microbial thallus, and determining effective viable count of 5.2 × 108cfu/g。
Preparation of biological organic fertilizer
The microbial thalli obtained above is adopted to prepare the following biological organic fertilizer.
Example 2.1
The biological organic fertilizer comprises the following components: 5g of Paenibacillus polymyxa (Paenibacillus polymyxa), 10g of Streptomyces microflavus (Streptomyces microflavus), 4g of Trichoderma asperellum and 81g of potassium humate.
The preparation method comprises the following steps: and respectively and uniformly mixing each microbial thallus with the same amount of potassium humate, and then uniformly mixing each obtained mixture with the rest potassium humate to obtain the biological organic fertilizer.
Example 2.2
The biological organic fertilizer comprises the following components: 5g of Paenibacillus polymyxa (Paenibacillus polymyxa), 15g of Streptomyces microflavus (Streptomyces microflavus), 3g of Trichoderma asperellum and 77g of sodium humate.
The preparation method is the same as example 2.1.
Example 2.3
The biological organic fertilizer comprises the following components: 5g of Paenibacillus polymyxa (Paenibacillus polymyxa), 10g of Streptomyces microflavus (Streptomyces microflavus), 4g of Trichoderma asperellum (Trichoderma asperellum), 8g of Azotobacter chroococcum (Azotobacter chroococcum) and 73g of potassium humate.
The preparation method is the same as example 2.1.
Example 2.4
The biological organic fertilizer comprises the following components: 3.75g of Paenibacillus polymyxa, 11.25g of Streptomyces microflavus, 2.25g of Trichoderma asperellum, 6.75g of Azotobacter chroococcum and 76g of potassium humate.
The preparation method is the same as example 2.1.
Example 2.5
The biological organic fertilizer comprises the following components: 3.75g of Paenibacillus polymyxa, 11.25g of Streptomyces microflavus, 2.25g of Trichoderma asperellum, 6.75g of Azotobacter chroococcum and 76g of sodium humate.
The preparation method is the same as example 2.1.
Example 2.6
The biological organic fertilizer comprises the following components: 6g of Paenibacillus polymyxa (Paenibacillus polymyxa), 6g of Streptomyces microflavus (Streptomyces microflavus), 3g of Trichoderma asperellum (Trichoderma asperellum), 9g of Azotobacter chroococcum (Azotobacter chroococcum) and 76g of potassium humate.
The preparation method is the same as example 2.1.
Example 2.7
The biological organic fertilizer comprises the following components: 3.75g of Paenibacillus polymyxa, 15g of Streptomyces microflavus, 3.75g of Trichoderma asperellum, 7.5g of Azotobacter chroococcum and 70g of potassium humate.
The preparation method is the same as example 2.1.
Comparative example 2.1
The biological organic fertilizer comprises the following components: 3.75g of Paenibacillus polymyxa, 11.25g of Streptomyces microflavus, 2.25g of Trichoderma asperellum, 6.75g of Azotobacter chroococcum and 76g of turfy soil.
The preparation method is the same as example 2.1.
Comparative example 2.2
The biological organic fertilizer comprises the following components: 8.6g of Paenibacillus polymyxa, 15.4g of Azotobacter chroococcum and 76g of potassium humate.
The preparation method is the same as example 2.1.
Comparative example 2.3
The biological organic fertilizer comprises the following components: 20g of Streptomyces microflavus, 4g of Trichoderma asperellum and 76g of potassium humate.
The preparation method is the same as example 2.1.
Comparative example 2.4
The biological organic fertilizer comprises the following components: 3.75g of Paenibacillus polymyxa, 11.25g of Streptomyces microflavus, 2.25g of Trichoderma asperellum and 6.75g of Azotobacter chroococcum.
The preparation method comprises the following steps: and uniformly mixing the microbial thalli to obtain the microbial inoculum.
Third, the activity of the plant root system is determined
Preparing a nutrition pot of 11cm multiplied by 13cm, and filling the nutrition pot with nutrition soil. Then, according to the processing arrangement shown in the following table 1, the bio-organic fertilizer and a proper amount of nutrient soil are uniformly mixed and applied to the periphery of the planting hole, and then tomato seedlings with consistent growth vigor are planted in nutrition pots, and 1 plant is planted in each nutrition pot. Each 10 tomato seedlings were treated. Watering after planting, and then carrying out daily illumination and watering treatment.
TABLE 1 application of the biological organic fertilizer
After the tomato seedlings grow for 1 month, selecting the tomato seedlings with good growth vigor respectively in each treatment, and measuring the root system vigor by adopting a TTC method (refer to main code of Haebin, etc. of plant physiology experiment technology, pages 46-47). The root systems of 3 tomatoes are respectively selected for each treatment, and the average value is obtained after 3 times of measurement. The specific determination method is as follows:
(1) preparation of TTC standard curve: 0.25ml of 0.4% TTC solution is sucked and put into a 10ml volumetric flask, a little Na is added2S2O4Powder, shaking up gives red TTF. Then ethyl acetate is added to the volume to be calibrated and shaken up. Then 0.25ml, 0.50 ml, 1.00 ml, 1.50 ml and 2.00ml of the solution are respectively taken and put into a 10ml volumetric flask, ethyl acetate is used for constant volume to reach the scale, thus obtaining the standard colorimetric series of TTC 25, TTC 50, TTC 100, TTC 150 and TTC 200 mug, and the light absorption value is measured under the wavelength of 485nm to draw a standard curve.
(2) Root sample determination: 0.5g of a root sample was weighed, placed in a petri dish, 10ml of a quantitative mixture of 0.4% TTC solution and phosphate buffer (1/15mol/L, pH 7.0) was added, the root was immersed sufficiently in the solution, and incubated in the dark at 37 ℃ for 1 hour, after which 2ml of 1mol/L sulfuric acid was added to stop the reaction. The blank test was performed by adding sulfuric acid first and then the sample, and the other operations were the same. Thereafter, the roots were taken out, dried by suction, and ground with ethyl acetate 3ml and a small amount of silica sand to extract TTF. Transferring the red extract into a test tube, washing the residue with a small amount of ethyl acetate for 2-3 times, transferring into the test tube, adding ethyl acetate to constant volume to 10ml, performing color comparison with a spectrophotometer at 485nm, reading out the light absorption value with blank as reference, and calculating the TTC reduction amount of the root system through a standard curve. The calculation method comprises the following steps:
root system activity [ μ g TTC/(g · h) ], TTC reduction amount (μ g)/[ root weight (g) × time (h) ]
The measurement results of the respective treatments according to the above methods are shown in table 2 below.
TABLE 2 determination of root activity
| Biological organic fertilizer | Root system activity [ mu g TTC/(g h)] | |
| Process 1 | Example 2.1 | 157.3 |
| Treatment 2 | Example 2.2 | 152.2 |
| Treatment 3 | Example 2.3 | 173.5 |
| Treatment 4 | Example 2.4 | 179.7 |
| Treatment 5 | Example 2.5 | 165.1 |
| Treatment 6 | Example 2.6 | 163.6 |
| Treatment 7 | Example 2.7 | 168.4 |
| Control 1 | Comparative example 2.1 | 132.7 |
| Control 2 | Comparative example 2.2 | 124.8 |
| Control 3 | Comparative example 2.3 | 130.0 |
| Control 4 | Comparative example 2.4 | 117.5 |
| Control 5 | Humic acid potassium salt | 108.4 |
| Blank control | / | 95.1 |
As can be seen from the results in Table 2, the activity of the tomato root system is significantly increased after the bio-organic fertilizer of the present invention is applied. Specifically, the microbial cells of the bio-organic fertilizer and the microbial cells and the potassium humate can generate synergistic effect by mixing, and the effect is obviously superior to the treatment of independently applying the microbial cells and the potassium humate (contrast 4-5), the effect is obviously superior to other microbial cell combinations (contrast 2-3) and the effect is superior to the combination of the microbial cells and the turfy soil (contrast 1). In addition, it is not difficult to see from the above results that, in the bio-organic fertilizer of the present invention, the treatment of potassium humate is superior to the treatment of sodium humate.
The above examples are merely illustrative and not restrictive of the present invention, and the solution obtained by simple modification of the above solution is also within the scope of the present application.
Claims (1)
1. The application of the bio-organic fertilizer for enhancing the activity of plant roots is characterized in that the bio-organic fertilizer comprises the following components in parts by weight: 3.75g of Paenibacillus polymyxa, 11.25g of Streptomyces microflavus, 2.25g of Trichoderma asperellum, 6.75g of Azotobacter chroococcum and 76g of potassium humate;
the plant is tomato;
the preparation method of the biological organic fertilizer comprises the following steps:
(1) preparation of microbial cells:
respectively carrying out slant culture, seed culture and fermentation culture on Paenibacillus polymyxa (Paenibacillus polymyxa), streptomyces microflavus (Streptomyces microflavus), Trichoderma asperellum and azotobacter chroococcum to obtain fermentation cultures; then obtaining microbial thalli through post-treatment;
(2) preparing a biological organic fertilizer:
uniformly mixing the microbial thallus obtained in the formula amount step (1) with the potassium humate in the formula amount to obtain the bio-organic fertilizer.
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